ORF
Human Ortholog
Description Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.21 0.11 0.1 0.1 0.25 0.15 0.13 0.15 0.12 0.06 0.1 0.13 0.18 0.16 0.05 0.07 0.05 0 0.06 0 0.06 0 0
Bud 0.08 0.08 0 0 0.07 0 0.05 0.05 0.06 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.71 0.56 0.64 0.64 0.53 0.53 0.48 0.36 0.38 0.2 0.32 0.74 0.83 0.78 0.85 0.85 0.84 0.79 0.55 0.75 0.8 0.9 0.85
Cytoplasm 0.35 0.55 0.57 0.59 0.41 0.55 0.54 0.59 0.54 0.56 0.64 0.43 0.35 0.37 0.15 0.14 0.18 0.05 0.21 0.07 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.05 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.15 0.14 0.1 0.3 0.34 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 53 51 34 36 124 69 72 58 41 23 52 26 45 49 25 24 18 12 29 9 6 4 9
Bud 20 34 13 6 33 14 26 18 21 42 23 8 4 12 6 3 2 2 2 0 0 0 3
Bud Neck 0 0 1 1 3 1 4 2 1 0 2 0 0 0 0 0 0 11 44 30 4 5 7
Bud Site 0 0 2 1 0 0 0 0 0 2 0 0 0 0 0 0 0
Cell Periphery 181 253 230 236 269 249 262 139 132 74 169 149 205 239 436 276 321 245 288 342 84 222 263
Cytoplasm 88 247 202 217 207 256 292 230 191 211 333 87 87 114 76 46 68 16 107 31 4 2 7
Endoplasmic Reticulum 12 19 2 7 12 16 24 9 3 1 11 9 9 17 2 4 5 9 26 14 0 4 5
Endosome 0 0 1 1 1 0 0 0 0 0 0 0 0 1 0 0 0 1 3 1 0 0 2
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 0 3 2 1 1 1 1 0 0 0
Mitochondria 2 4 2 10 23 69 78 37 105 127 92 1 1 0 9 2 3 4 9 10 1 3 3
Nucleus 0 1 4 2 2 2 3 5 4 6 4 2 2 2 22 5 19 0 0 0 0 0 0
Nuclear Periphery 2 0 0 0 1 0 0 2 0 7 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 2 6 0 0 0 0 0 0 0 0 0
SpindlePole 4 2 5 6 9 3 3 4 1 5 7 0 0 0 1 2 4 0 0 0 0 0 0
Vac/Vac Membrane 0 0 6 2 6 0 2 4 0 9 10 0 0 2 8 1 5 1 5 7 0 1 1
Unique Cell Count 254 448 357 369 503 466 545 387 351 379 521 201 247 307 511 324 382 311 522 457 106 246 310
Labelled Cell Count 363 611 503 525 690 679 766 508 500 508 705 283 355 442 588 365 446 311 522 457 106 246 310


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 8.2 7.6 7.1 7.4 6.0 6.0 6.4 5.3 5.0 5.8 8.3 8.3 8.2 15.2 15.2 14.6 7.4 7.2 7.7
Std Deviation (1e-4) 1.1 1.3 0.8 0.9 1.0 0.9 0.7 0.8 0.8 0.9 0.8 1.1 1.4 1.0 2.6 2.7 2.4 1.3 1.1 1.3
Intensity Change (Log2) -0.08 -0.04 -0.33 -0.33 -0.24 -0.5 -0.59 -0.38 0.12 0.14 0.11 1.01 1.01 0.95 -0.03 -0.07 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.1 5.6 2.3 1.7 2.3 0.9 -1.8 0.2 1.2 3.1 2.5 -2.7 -1.0 -2.6
Bud -1.7 1.9 -0.5 0.8 0.7 1.5 3.8 0.6 0.2 -1.5 0.2 -2.4 -2.3 -3.0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -3.2 -3.2 -4.8 -7.8 -7.1 -12.4 -9.4 2.4 5.0 3.8 7.2 6.2 6.1
Cytoplasm 0.6 -4.5 -0.5 -0.9 0.8 -0.6 -0.2 2.2 -3.0 -5.2 -5.0 -13.0 -11.5 -10.9
Endoplasmic Reticulum 0 2.1 2.8 3.4 2.0 0 0 1.9 0 0 3.8 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.3 3.5 7.2 7.1 5.5 10.9 11.8 8.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 2.7 0 3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.2 0.4 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0.7 0.3 0 0 0 -0.1 0 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4152 3.5633 3.3763 2.9743 2.733 3.5304 5.0699 5.4276 5.2286 4.4858 3.9967 5.0332 6.223 5.4919 5.4244 5.3459 5.1532 5.4263
Actin 0.0569 0.0134 0.0292 0.0073 0.066 0.0178 0.0144 0.0006 0.0046 0.0115 0.0342 0.0036 0.0094 0.0068 0.0028 0.0172 0.0002 0.0025
Bud 0.0011 0.0011 0.0026 0.0023 0.0008 0.0011 0.0007 0.0002 0.0007 0.0005 0.0021 0.0004 0.0003 0.0007 0.0005 0.0006 0.0004 0.0004
Bud Neck 0.0141 0.0044 0.0217 0.0141 0.0373 0.0426 0.0032 0.0021 0.0041 0.0067 0.0233 0.0245 0.0079 0.0041 0.0092 0.0106 0.0055 0.0269
Bud Periphery 0.0066 0.0066 0.0192 0.0283 0.0087 0.0121 0.0034 0.0013 0.0059 0.0055 0.0131 0.0079 0.0017 0.0032 0.0082 0.0087 0.0056 0.007
Bud Site 0.0179 0.0938 0.057 0.0032 0.0026 0.0033 0.0054 0.0643 0.0177 0.0064 0.0076 0.0008 0.0056 0.0868 0.0227 0.0027 0.0107 0.0012
Cell Periphery 0.5454 0.387 0.4248 0.572 0.5023 0.5281 0.6927 0.6616 0.7047 0.6846 0.5331 0.7538 0.6989 0.535 0.6486 0.6567 0.7683 0.7054
Cytoplasm 0.2181 0.4498 0.381 0.3461 0.2637 0.344 0.1864 0.2573 0.2283 0.1814 0.1616 0.1826 0.2199 0.3179 0.282 0.2491 0.1826 0.2349
Cytoplasmic Foci 0.0085 0.0018 0.0048 0.0003 0.0089 0.0024 0.0096 0.0001 0.0032 0.0098 0.0108 0.0009 0.0012 0.0008 0.0003 0.0012 0.0001 0.0009
Eisosomes 0.0059 0.0019 0.0032 0.0012 0.0023 0.001 0.0184 0.0014 0.001 0.015 0.0063 0.0011 0.0077 0.0014 0.0018 0.0015 0.0004 0.0005
Endoplasmic Reticulum 0.0241 0.0141 0.0178 0.0166 0.0295 0.018 0.0119 0.008 0.0153 0.0246 0.0253 0.0125 0.0232 0.0282 0.0159 0.0244 0.0205 0.0115
Endosome 0.022 0.0005 0.0014 0.0001 0.0241 0.0045 0.0181 0 0.0024 0.0025 0.0457 0.0003 0.001 0.0029 0 0.0008 0 0.0007
Golgi 0.0153 0.0011 0.0029 0.0004 0.0111 0.0067 0.0064 0.0001 0.0025 0.0043 0.0308 0.0008 0.0012 0.0012 0.0003 0.0019 0.0001 0.0004
Lipid Particles 0.0081 0.0018 0.0104 0.0011 0.0133 0.0034 0.0096 0.0005 0.002 0.0142 0.0069 0.0012 0.0024 0.004 0.0008 0.0033 0.0009 0.001
Mitochondria 0.0055 0.0001 0.0015 0 0.0025 0.002 0.0091 0 0.0014 0.0091 0.052 0.0047 0.0034 0.0001 0 0.0113 0 0.0004
None 0.0153 0.0178 0.0146 0.0016 0.0061 0.0043 0.0015 0.0003 0.0007 0.002 0.0012 0.0003 0.0052 0.0013 0.0038 0.0051 0.0005 0.0011
Nuclear Periphery 0.0133 0.001 0.0008 0.0006 0.0061 0.0026 0.002 0.0004 0.0005 0.0009 0.0022 0.0003 0.0009 0.0011 0.0005 0.0006 0.0005 0.0018
Nucleolus 0.0007 0.0002 0.0002 0.0001 0.0004 0.0004 0.0002 0 0.0001 0.0002 0.0002 0 0.0002 0.0001 0 0.0001 0 0.0001
Nucleus 0.0083 0.0006 0.0006 0.0004 0.0011 0.001 0.0004 0.0002 0.0002 0.0003 0.0007 0.0002 0.0004 0.0005 0.0002 0.0003 0.0002 0.0005
Peroxisomes 0.0034 0.0001 0.0016 0 0.0041 0.0003 0.0026 0 0.0024 0.0167 0.0269 0.0016 0.0062 0 0 0.0007 0 0
Punctate Nuclear 0.0046 0.0002 0.0002 0 0.0053 0.0012 0.0009 0 0.0002 0.0002 0.0003 0 0.0003 0.0001 0 0.0001 0 0.0002
Vacuole 0.004 0.0028 0.0042 0.004 0.0029 0.0029 0.0028 0.0014 0.0018 0.0034 0.0085 0.0024 0.0029 0.0037 0.0024 0.0028 0.0036 0.0028
Vacuole Periphery 0.001 0.0001 0.0001 0.0001 0.0007 0.0003 0.0004 0 0.0001 0.0002 0.0073 0.0001 0.0002 0.0001 0 0.0002 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.4587 22.0131 22.5905 27.8424 22.0773 21.4694 27.8218 29.2455 29.4474 29.1363
Translational Efficiency 0.904 1.2233 1.2229 1.08 1.0835 1.0952 1.1656 1.0049 0.9161 0.8541

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1843 538 118 817 1840 1369 159 141 3683 1907 277 958

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 813.50 1015.98 1288.08 1305.70 918.04 991.22 1310.63 1359.22 865.73 998.21 1301.02 1313.58
Standard Deviation 93.10 143.19 146.56 218.00 122.09 140.58 166.12 192.11 120.49 141.76 158.48 215.22
Intensity Change Log 2 0.320658 0.663008 0.682609 0.110648 0.513632 0.566150 0.213129 0.585740 0.622037

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007601 0.010187 0.054045 0.065553 0.007342 0.016529 0.062047 0.068590 0.007472 0.014740 0.058639 0.066000
Bud Neck 0.071903 0.036077 0.058226 0.063279 0.047017 0.059086 0.045262 0.044699 0.059470 0.052595 0.050784 0.060544
Bud Site 0.053082 0.113877* 0.205924* 0.234912* 0.063062 0.096167 0.192874* 0.272483* 0.058068 0.101164* 0.198433* 0.240442*
Cell Periphery 0.184592* 0.399865* 0.166223* 0.219159* 0.244223* 0.346218* 0.157769* 0.194333* 0.214383* 0.361353* 0.161370* 0.215505*
Cytoplasm 0.535663* 0.382786* 0.115300* 0.154104* 0.552256* 0.397859* 0.123301* 0.146152* 0.543953* 0.393607* 0.119893* 0.152934*
Cytoplasmic Foci 0.014954 0.005725 0.029093 0.004574 0.008543 0.011067 0.026722 0.005458 0.011751 0.009560 0.027732 0.004704
Eisosomes 0.050330 0.009590 0.009306 0.046872 0.025904 0.023309 0.023688 0.033167 0.038127 0.019439 0.017561 0.044855
Endoplasmic Reticulum 0.009040 0.013171 0.115303* 0.017544 0.009619 0.007600 0.051795 0.021321 0.009329 0.009171 0.078849 0.018100
Endosome 0.001474 0.001157 0.008244 0.002704 0.001138 0.002010 0.004312 0.000830 0.001306 0.001770 0.005987 0.002428
Golgi 0.005778 0.003562 0.005319 0.003680 0.004180 0.005532 0.001273 0.000339 0.004980 0.004976 0.002997 0.003189
Lipid Particles 0.005077 0.002029 0.021762 0.009009 0.003421 0.002369 0.009400 0.003807 0.004249 0.002273 0.014666 0.008244
Mitochondria 0.003585 0.001124 0.000270 0.000967 0.001518 0.001639 0.000124 0.000151 0.002552 0.001494 0.000186 0.000847
Mitotic Spindle 0.000283 0.000264 0.000441 0.002206 0.000284 0.000580 0.000271 0.000145 0.000283 0.000491 0.000343 0.001903
None 0.001896 0.000080 0.000049 0.000300 0.000507 0.000120 0.000130 0.002103 0.001202 0.000108 0.000095 0.000565
Nuclear Periphery 0.000148 0.000138 0.000328 0.000217 0.000146 0.000120 0.000277 0.000157 0.000147 0.000125 0.000299 0.000208
Nuclear Periphery Foci 0.000695 0.000917 0.009343 0.001052 0.000697 0.000609 0.007001 0.001573 0.000696 0.000696 0.007999 0.001129
Nucleolus 0.000165 0.000040 0.000039 0.000028 0.000072 0.000080 0.000046 0.000016 0.000119 0.000069 0.000043 0.000026
Nucleus 0.029791 0.003223 0.026065 0.054071 0.010801 0.006435 0.032422 0.037643 0.020304 0.005528 0.029714 0.051653
Peroxisomes 0.006860 0.003397 0.000728 0.000477 0.004260 0.003339 0.000499 0.000308 0.005561 0.003355 0.000597 0.000452
Vacuole 0.016753 0.012407 0.173754 0.117716 0.014727 0.018922 0.260704* 0.164162 0.015741 0.017084 0.223664* 0.124552
Vacuole Periphery 0.000330 0.000384 0.000239 0.001574 0.000282 0.000412 0.000084 0.002562 0.000306 0.000404 0.000150 0.001720

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.59 -7.10 -17.90 -16.67 -2.10 -6.84 -6.31 -7.19 -6.02 -0.96 -7.24 -9.07 -19.38 -16.30 -2.09
Bud Neck 8.40 1.68 2.50 -5.89 -0.62 -3.35 1.11 1.68 4.09 0.20 2.38 2.36 0.31 -1.72 -2.04
Bud Site -7.65 -8.84 -22.18 -10.95 -1.71 -6.63 -7.20 -10.37 -8.46 -3.19 -10.44 -11.18 -24.19 -16.73 -3.51
Cell Periphery -15.08 1.62 -2.78 12.54 -2.85 -10.62 6.67 4.63 9.72 -1.01 -18.91 5.82 1.67 16.69 -4.32
Cytoplasm 11.89 28.58 48.64 17.42 -2.57 17.60 32.68 23.53 14.12 -1.38 21.48 43.47 57.93 29.81 -3.10
Cytoplasmic Foci 7.25 -3.65 7.71 0.61 5.93 -1.81 -4.88 1.43 2.48 5.16 1.95 -5.92 7.07 3.58 7.98
Eisosomes 21.82* 21.25 1.85 -13.66 -13.63 1.69 1.86 -0.36 -0.97 -1.40 14.41 11.96 -1.86 -9.99 -10.03
Endoplasmic Reticulum -1.98 -7.25 -5.81 -1.99 6.62 1.71 -6.49 -3.26 -3.86 4.04 0.15 -9.17 -6.77 -6.30 7.84
Endosome 1.00 -6.75 -1.29 -1.67 4.54 -2.59 -5.45 1.01 2.94 5.58 -1.82 -8.44 -1.40 -0.71 4.32
Golgi 2.09 0.95 1.84 0.10 1.15 -1.52 6.83 9.07 6.60 4.22 0.00 4.95 1.89 1.68 -0.17
Lipid Particles 5.90 -5.90 -3.27 -5.63 3.94 2.40 -6.06 -0.99 -2.65 4.85 5.72 -7.59 -3.92 -5.70 3.38
Mitochondria 5.35 9.05 5.72 0.48 -2.23 -0.43 9.19 9.05 6.22 -0.90 3.88 11.93 5.47 2.17 -2.53
Mitotic Spindle 0.34 -2.25 -1.75 -1.76 -1.61 -1.13 -1.10 2.20 1.59 1.50 -1.11 -1.41 -1.72 -1.44 -1.40
None 8.99 9.16 7.74 -4.79 -5.63 5.78 4.28 -0.77 -1.02 -0.99 10.17 9.88 2.77 -1.72 -1.68
Nuclear Periphery 0.52 -5.34 -5.75 -3.58 3.15 2.31 -4.51 -1.30 -2.68 3.73 2.27 -6.79 -6.08 -6.54 3.96
Nuclear Periphery Foci -0.83 -7.99 -4.41 -0.81 7.55 1.09 -7.86 -3.03 -3.23 6.20 -0.05 -11.17 -5.48 -4.09 10.28
Nucleolus 6.36 6.75 7.55 1.56 2.66 -0.31 1.83 15.84 1.93 2.12 1.83 6.13 9.91 1.81 2.11
Nucleus 20.46 0.62 -9.48 -19.64 -7.14 6.53 -6.74 -7.51 -8.50 -1.66 19.30 -4.42 -14.13 -20.41 -7.13
Peroxisomes 6.12 14.19 15.16 7.62 2.50 1.78 9.96 10.64 8.35 2.39 5.58 17.28 18.09 10.28 2.31
Vacuole 2.49 -9.17 -23.15 -23.02 3.40 -3.44 -13.72 -10.96 -10.71 3.32 -1.42 -16.11 -25.07 -24.45 7.53
Vacuole Periphery -0.59 1.61 -1.27 -1.21 -1.38 -1.62 5.08 -0.88 -0.81 -0.98 -1.53 4.49 -1.52 -1.39 -1.72
ORF
Human Ortholog
Description Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cell periphery (57%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

YGR266W

YGR266W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr266w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available