Standard name
Human Ortholog
Description Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.08 0.08 0.14 – 0.12 0.08 0.09 0 0.05 0.11 0.15 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.16 0 – 0.06 0.05 0.08 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.87 0.91 0.91 0.94 0.87 0.71 0.74 – 0.65 0.75 0.66 0.64 0.88 0.82 0.8 0.85 0.87 0.79 0.73 0.88 0.73 0.7 0.67 0.68
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.35 0.16 0.13 0.14 0.1 0.33 0.21 – 0.33 0.37 0.45 0.59 0.2 0.13 0.11 0.19 0.15 0.2 0.19 0.07 0.17 0.23 0.26 0.22
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 – 1 1 0 0 0 0 0 1 0 0 3 5 1 2 2 7
Bud Neck 0 0 0 0 0 1 0 – 2 1 0 1 0 0 0 1 1 0 0 1 0 0 0 1
Bud Site 2 2 0 0 0 2 0 – 4 4 5 3 0 0 1 0 0 3 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0
Cytoplasm 7 10 0 5 11 13 34 – 21 14 11 4 25 102 129 6 5 13 0 0 0 0 1 2
Endoplasmic Reticulum 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 1
Endosome 2 1 1 0 0 0 0 – 0 0 0 0 0 4 3 1 0 0 7 1 3 3 3 4
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 4 0 0 1 0 0
Mitochondria 9 18 1 1 1 25 7 – 10 9 10 9 2 0 0 8 9 14 3 2 1 0 1 2
Nucleus 383 450 63 130 127 110 178 – 111 138 84 77 430 748 686 240 244 204 355 455 87 339 471 466
Nuclear Periphery 1 1 0 1 2 1 1 – 2 1 0 0 0 1 4 2 3 3 0 0 0 0 0 1
Nucleolus 153 81 9 19 14 51 52 – 56 69 57 71 100 117 95 55 43 51 92 37 20 114 184 153
Peroxisomes 1 1 0 0 0 0 0 – 0 0 0 0 0 5 7 0 0 0 0 0 0 0 0 0
SpindlePole 3 0 1 2 1 0 1 – 2 1 0 1 5 14 14 5 1 3 3 1 0 5 7 10
Vac/Vac Membrane 2 4 0 0 0 0 5 – 0 1 0 0 1 4 4 3 6 5 10 10 2 18 24 27
Unique Cell Count 439 497 69 139 146 154 242 170 185 127 121 490 909 862 284 279 257 485 519 121 488 699 685
Labelled Cell Count 564 568 75 158 157 204 278 209 239 167 166 563 996 945 322 312 299 485 519 121 488 699 685


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 8.0 8.5 8.0 9.0 6.0 8.3 – 7.5 7.8 7.6 7.2 8.8 8.7 9.2 10.9 11.4 10.3 7.2 8.1 8.4
Std Deviation (1e-4) 2.3 1.7 1.9 1.5 2.1 1.7 2.3 – 2.2 2.7 2.0 1.8 2.1 2.3 2.6 3.3 3.5 3.8 1.7 2.0 2.0
Intensity Change (Log2) – – – -0.09 0.08 -0.49 -0.03 – -0.19 -0.13 -0.17 -0.25 0.04 0.03 0.11 0.36 0.42 0.27 -0.25 -0.06 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 3.3 – 3.1 0 0 0 0 2.9 3.5 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.2 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0.6 -0.9 -3.3 -3.1 – -4.1 -2.9 -3.9 -4.2 -0.9 -1.9 -2.4 -1.5 -0.9 -2.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 -0.8 3.1 1.6 – 3.1 3.7 4.5 6.1 1.4 0 -0.5 1.2 0.5 1.3
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4537 1.6685 1.6916 1.2909 1.4036 1.8506 4.1431 3.4679 3.2483 3.3712 3.2523 3.6789 6.7734 6.4203 6.5278 7.007 6.0779 6.3892
Actin 0.009 0 0.0002 0 0.0005 0.0005 0.023 0.0004 0.0098 0.0278 0.0057 0.0003 0.0159 0 0.0009 0.0001 0.0068 0.0001
Bud 0.0001 0 0 0 0.0001 0 0.0002 0.0001 0.0002 0.0007 0.0004 0.0001 0.0005 0 0.0006 0.0001 0.0005 0
Bud Neck 0.0007 0 0.0001 0.0001 0.0007 0.0013 0.001 0.0002 0.0002 0.0006 0.0004 0.0004 0.0053 0.0001 0.0005 0.0006 0.0007 0.0005
Bud Periphery 0.0003 0 0.0001 0.0001 0.0001 0 0.0004 0.0003 0.0002 0.0018 0.001 0.0002 0.0011 0 0.0009 0.0001 0.0007 0
Bud Site 0.0003 0 0.0001 0.0001 0.0003 0.0001 0.0015 0.0023 0.001 0.0025 0.0007 0.0001 0.0019 0 0.0015 0.0001 0.003 0
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0.0001 0.0005 0.0001 0.0001 0.0002 0 0.0001 0 0.0002 0
Cytoplasm 0.0011 0.0001 0.0009 0 0.0221 0.0003 0.0023 0 0.0012 0.0002 0.0004 0.0002 0.0037 0 0.0094 0.0171 0.0004 0.0001
Cytoplasmic Foci 0.0132 0 0.0004 0 0.001 0.0005 0.0097 0 0.0042 0.0025 0.0035 0.0002 0.0044 0 0.0064 0.0001 0.0036 0.0015
Eisosomes 0.0001 0 0 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0011 0 0.0003 0 0.0018 0.0003 0.004 0 0.0013 0.0006 0.002 0.0006 0.0029 0 0.0008 0.0007 0.0009 0.0002
Endosome 0.0057 0 0.0012 0 0.0026 0.0032 0.0106 0.0001 0.0054 0.0128 0.0262 0.0028 0.013 0 0.0036 0.0002 0.0042 0.0007
Golgi 0.0018 0 0.0001 0 0.0001 0.0004 0.0056 0.0001 0.0032 0.0047 0.0111 0.0006 0.0034 0 0.0004 0 0.0021 0.0001
Lipid Particles 0.0134 0 0.0002 0 0.0001 0.0006 0.0092 0.0001 0.0036 0.008 0.0068 0.0028 0.0133 0 0.0016 0 0.0076 0.0005
Mitochondria 0.0019 0 0.0004 0.0002 0.0008 0.0005 0.0026 0.0037 0.0012 0.0092 0.0527 0.0008 0.0072 0 0.0021 0.0001 0.0021 0.0003
None 0.0011 0 0.0003 0 0.002 0.0002 0.004 0 0.0011 0.0002 0.0002 0.0003 0.0018 0 0.0014 0.02 0.0009 0
Nuclear Periphery 0.0132 0.0007 0.0036 0.0003 0.0054 0.0026 0.0231 0.0004 0.0029 0.0016 0.0096 0.0035 0.0176 0.0003 0.0039 0.0019 0.0036 0.0029
Nucleolus 0.0339 0.0258 0.0193 0.0417 0.1291 0.073 0.02 0.0257 0.0181 0.0221 0.1444 0.0741 0.0438 0.0322 0.0252 0.0466 0.1594 0.058
Nucleus 0.876 0.9682 0.9673 0.9561 0.7937 0.913 0.8688 0.9585 0.934 0.8898 0.705 0.911 0.8441 0.9662 0.9324 0.9053 0.7952 0.9282
Peroxisomes 0.0103 0 0.0001 0 0.0001 0.0001 0.0038 0.0001 0.0074 0.0038 0.0005 0 0.0019 0 0.0007 0 0.0011 0.0001
Punctate Nuclear 0.0146 0.0051 0.0048 0.0011 0.0385 0.0028 0.0071 0.0072 0.0033 0.002 0.026 0.0008 0.0143 0.0011 0.0062 0.0066 0.0051 0.0066
Vacuole 0.0011 0 0.0003 0.0001 0.0005 0.0002 0.0017 0.0003 0.0013 0.0062 0.0012 0.0006 0.0024 0 0.0012 0.0003 0.0011 0.0002
Vacuole Periphery 0.0009 0 0.0002 0 0.0003 0.0002 0.0012 0.0001 0.0004 0.0022 0.0017 0.0005 0.0012 0 0.0004 0 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.0818 31.2144 38.6041 35.8671 45.2725 34.8985 28.4048 27.6919 40.7704 39.2321
Translational Efficiency 3.4712 2.7409 1.888 2.2523 2.5192 3.4113 2.2164 2.8647 1.6635 2.576

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1749 1076 1626 87 1577 1869 96 840 3326 2945 1722 927

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 748.78 964.02 1085.72 1338.94 832.58 944.50 1254.31 1147.89 788.51 951.63 1095.12 1165.82
Standard Deviation 121.82 171.02 173.40 285.26 125.45 149.27 242.94 214.35 130.44 157.85 182.15 228.86
Intensity Change Log 2 — 0.364521 0.536038 0.838478 — 0.181962 0.591233 0.463324 — 0.271288 0.565361 0.653142

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000498 0.000821 0.000769 0.001247 0.000390 0.000558 0.000620 0.001081 0.000447 0.000654 0.000761 0.001096
Bud Neck 0.006422 0.011897 0.024637 0.009338 0.007299 0.014042 0.011355 0.024147 0.006838 0.013258 0.023897 0.022758
Bud Site 0.001342 0.002118 0.001319 0.001592 0.000610 0.001692 0.000640 0.003189 0.000995 0.001848 0.001281 0.003039
Cell Periphery 0.000193 0.000098 0.000044 0.000106 0.000078 0.000102 0.000033 0.000106 0.000139 0.000100 0.000044 0.000106
Cytoplasm 0.003333 0.000937 0.000207 0.000637 0.000371 0.000916 0.000285 0.001368 0.001929 0.000924 0.000211 0.001300
Cytoplasmic Foci 0.000132 0.000686 0.000016 0.000295 0.000051 0.000447 0.000060 0.001548 0.000094 0.000534 0.000019 0.001430
Eisosomes 0.000029 0.000056 0.000058 0.000083 0.000035 0.000057 0.000042 0.000054 0.000032 0.000057 0.000057 0.000057
Endoplasmic Reticulum 0.003372 0.002618 0.001316 0.001314 0.002400 0.002463 0.000832 0.001748 0.002912 0.002520 0.001289 0.001707
Endosome 0.000255 0.000790 0.000061 0.000073 0.000122 0.001137 0.000312 0.002693 0.000192 0.001010 0.000075 0.002447
Golgi 0.000183 0.000330 0.000108 0.000145 0.000098 0.000434 0.000161 0.002311 0.000142 0.000396 0.000111 0.002107
Lipid Particles 0.000121 0.000121 0.000017 0.000037 0.000119 0.000380 0.000030 0.000389 0.000120 0.000285 0.000017 0.000356
Mitochondria 0.001018 0.002755 0.001669 0.002309 0.000673 0.001616 0.001387 0.004188 0.000854 0.002033 0.001653 0.004012
Mitotic Spindle 0.000666 0.001519 0.000848 0.001170 0.001072 0.005580 0.000269 0.009627 0.000859 0.004097 0.000816 0.008833
None 0.011730 0.004528 0.001122 0.001670 0.001539 0.002903 0.001078 0.003270 0.006898 0.003497 0.001119 0.003120
Nuclear Periphery 0.000266 0.000157 0.000167 0.000218 0.000365 0.000349 0.000606 0.001116 0.000313 0.000279 0.000191 0.001032
Nuclear Periphery Foci 0.000760 0.000370 0.000036 0.000034 0.000581 0.000305 0.001043 0.001027 0.000675 0.000329 0.000092 0.000934
Nucleolus 0.067872 0.121103 0.063157 0.059744 0.077179 0.100943 0.061125 0.074337 0.072285 0.108309 0.063044 0.072967
Nucleus 0.900737* 0.845885* 0.902935* 0.913994* 0.905994* 0.862426* 0.917497* 0.861881* 0.903230* 0.856382* 0.903747* 0.866771*
Peroxisomes 0.000451 0.001653 0.001030 0.005081 0.000562 0.001108 0.001278 0.001352 0.000504 0.001307 0.001044 0.001702
Vacuole 0.000293 0.000806 0.000295 0.000685 0.000229 0.001247 0.000714 0.002800 0.000263 0.001086 0.000318 0.002601
Vacuole Periphery 0.000326 0.000750 0.000187 0.000227 0.000234 0.001294 0.000632 0.001769 0.000282 0.001096 0.000212 0.001624

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.48 -10.71 -6.24 -4.02 -3.89 -3.91 -3.90 -11.20 -8.72 -4.62 -6.12 -14.24 -11.99 -8.49 -5.80
Bud Neck -7.17 -21.61 -3.87 -0.44 8.32 -7.70 -3.09 -11.15 -6.88 -5.02 -10.47 -22.15 -11.83 -7.47 0.68
Bud Site -1.60 -1.25 -1.83 -0.66 -1.49 -3.85 -1.56 -5.06 -3.26 -4.10 -3.08 -3.80 -4.68 -3.04 -3.69
Cell Periphery 2.40 4.39 1.34 -0.62 -2.05 -0.50 0.96 -0.61 -0.21 -4.08 1.28 3.42 1.05 -0.41 -4.88
Cytoplasm 3.12 4.91 3.91 1.25 -1.68 -1.75 0.60 -3.25 -1.79 -3.61 1.98 4.95 -0.16 -1.78 -3.92
Cytoplasmic Foci -1.34 4.16 -1.70 0.57 -2.39 -2.16 0.06 -2.38 -1.41 -2.39 -2.35 4.27 -2.40 -1.21 -2.61
Eisosomes -9.74 -17.01 -6.44 -3.54 -3.28 -6.58 -2.47 -8.97 0.44 -1.14 -10.81 -16.01 -13.05 -0.78 -0.07
Endoplasmic Reticulum 3.16 11.45 2.47 1.16 -1.10 -0.41 8.08 1.98 2.30 -4.29 2.57 13.31 4.46 2.79 -2.80
Endosome -1.38 6.28 3.91 1.75 -1.25 -2.43 -0.54 -2.24 -0.97 -2.02 -2.74 4.46 -2.16 -1.04 -2.30
Golgi -1.91 1.31 -0.96 1.30 -2.50 -1.83 -0.84 -1.68 -1.33 -1.61 -2.18 0.62 -1.65 -1.35 -1.67
Lipid Particles -0.02 2.10 0.73 0.63 -2.11 -1.53 1.80 -0.99 0.56 -2.13 -1.61 2.77 -1.22 0.14 -2.25
Mitochondria -1.73 -5.14 -6.14 0.14 -2.68 -3.63 -3.84 -4.59 -3.09 -3.11 -2.93 -7.99 -4.57 -2.38 -2.93
Mitotic Spindle -1.33 -1.83 -1.62 -1.14 -1.23 -2.94 1.38 -4.23 -2.12 -4.81 -3.42 -0.94 -4.63 -2.86 -4.43
None 4.07 8.89 8.00 2.05 -1.64 -2.95 1.21 -3.05 -0.53 -3.84 4.03 8.77 4.76 0.57 -3.95
Nuclear Periphery 2.26 0.78 -1.17 -2.59 -1.73 0.04 -0.34 -1.62 -1.83 -0.84 0.27 0.72 -1.93 -2.08 -2.22
Nuclear Periphery Foci 2.10 5.36 5.22 3.41 -0.94 0.68 -0.13 -0.38 -1.35 -0.09 1.42 3.17 -0.28 -1.33 -2.50
Nucleolus -12.34 0.09 0.43 5.01 0.40 -6.44 1.09 -0.59 4.02 -1.29 -13.05 1.61 -1.43 6.38 -2.33
Nucleus 10.07 1.73 0.10 -3.81 -0.43 9.32 -0.41 7.37 1.37 4.15 13.24 2.35 7.16 0.01 5.63
Peroxisomes -8.27 -6.69 -4.33 -3.24 -3.73 -1.66 -2.32 -5.10 -0.73 0.23 -3.73 -5.73 -7.90 -1.73 -3.95
Vacuole -2.25 -1.63 -2.09 -1.31 -1.88 -3.26 -1.19 -3.06 -2.03 -2.38 -3.94 -1.88 -3.22 -2.27 -3.02
Vacuole Periphery -1.85 1.89 0.79 2.17 -2.11 -2.79 -0.77 -2.55 -0.22 -1.36 -3.18 1.38 -2.45 -0.45 -2.60
Standard name
Human Ortholog
Description Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rtt102

Rtt102


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtt102-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available