Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable pantetheine-phosphate adenylyltransferase (PPAT); PPAT catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT) and by human COASY |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 |
Bud | 0 | 1 | 0 | 0 | 1 | 0 | 0 | – | 1 | 2 | 8 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 4 | 4 | 1 | 1 | 4 | – | 1 | 3 | 6 | 10 | 1 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 225 | 351 | 264 | 188 | 194 | 136 | 213 | – | 111 | 149 | 398 | 468 | 541 | 555 | 689 | 239 | 197 | 225 | 227 | 342 | 254 | 476 | 440 | 369 |
Endoplasmic Reticulum | 0 | 4 | 1 | 0 | 1 | 2 | 2 | – | 0 | 2 | 3 | 6 | 1 | 0 | 0 | 11 | 6 | 5 | 0 | 1 | 1 | 1 | 3 | 0 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 3 | 1 | 2 | 3 | 3 |
Golgi | 2 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 0 | 6 | 0 | 0 | 7 | 9 | 19 | – | 65 | 56 | 159 | 174 | 0 | 0 | 0 | 7 | 3 | 2 | 0 | 8 | 2 | 1 | 1 | 2 |
Nucleus | 0 | 2 | 0 | 2 | 4 | 4 | 7 | – | 6 | 4 | 13 | 17 | 0 | 2 | 2 | 0 | 4 | 11 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 |
Vac/Vac Membrane | 4 | 1 | 1 | 1 | 0 | 2 | 15 | – | 6 | 7 | 26 | 26 | 1 | 1 | 5 | 4 | 9 | 9 | 2 | 2 | 0 | 2 | 1 | 2 |
Unique Cell Count | 231 | 359 | 267 | 189 | 198 | 143 | 241 | 162 | 200 | 540 | 630 | 544 | 557 | 693 | 246 | 206 | 232 | 237 | 364 | 267 | 496 | 460 | 395 | |
Labelled Cell Count | 232 | 368 | 272 | 195 | 209 | 154 | 260 | 191 | 227 | 614 | 713 | 545 | 561 | 697 | 264 | 221 | 255 | 237 | 364 | 267 | 496 | 460 | 395 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 5.3 | 6.9 | 6.4 | 5.4 | 5.2 | 5.7 | – | 4.7 | 5.2 | 5.0 | 5.2 | 7.0 | 6.9 | 6.4 | 6.4 | 8.0 | 7.3 | 7.4 | 7.4 | 7.5 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.0 | 1.0 | 1.2 | 0.7 | 1.5 | – | 1.1 | 1.2 | 0.9 | 0.9 | 0.9 | 1.2 | 1.1 | 1.4 | 1.8 | 1.6 | 1.3 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.34 | -0.41 | -0.26 | – | -0.54 | -0.41 | -0.46 | -0.41 | 0.03 | -0.0 | -0.1 | -0.11 | 0.22 | 0.08 | 0.11 | 0.11 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | -0.8 | -2.4 | -4.9 | – | -9.2 | -8.2 | -8.8 | -8.7 | 0.9 | 1.3 | 0.9 | -1.4 | -2.2 | -1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.7 | – | 0 | 9.2 | 9.9 | 9.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 3.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.8 | – | 0 | 0 | 3.3 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.781 | 6.2482 | 5.8006 | 5.4413 | 5.4715 | 5.9423 | 6.786 | 6.4911 | 6.2431 | 5.9673 | 7.0581 | 6.5121 | 3.217 | 3.5847 | 3.4029 | 3.4245 | 3.3846 | 3.3166 |
Actin | 0.0547 | 0.0062 | 0.0166 | 0.0733 | 0.0795 | 0.0054 | 0.041 | 0.0061 | 0.0133 | 0.0028 | 0.0345 | 0.0085 | 0.0395 | 0.0013 | 0.0126 | 0.0111 | 0.0614 | 0.0098 |
Bud | 0.0012 | 0.0002 | 0.0032 | 0.0003 | 0.0008 | 0.002 | 0.0008 | 0.006 | 0.0026 | 0.0069 | 0.0051 | 0.0007 | 0.0012 | 0.0084 | 0.0016 | 0.0064 | 0.003 | 0.0061 |
Bud Neck | 0.0149 | 0.0001 | 0.0006 | 0.0002 | 0.0004 | 0.0018 | 0.0052 | 0.0007 | 0.0035 | 0.0283 | 0.001 | 0.003 | 0.0163 | 0.0003 | 0.0103 | 0.0342 | 0.0115 | 0.0012 |
Bud Periphery | 0.0018 | 0.0003 | 0.0009 | 0.0002 | 0.0016 | 0.0007 | 0.0011 | 0.0075 | 0.002 | 0.0111 | 0.0069 | 0.0012 | 0.0018 | 0.0018 | 0.0034 | 0.0066 | 0.004 | 0.003 |
Bud Site | 0.005 | 0.0012 | 0.0026 | 0.0003 | 0.0006 | 0.0012 | 0.0054 | 0.0174 | 0.0112 | 0.0061 | 0.0097 | 0.0004 | 0.0081 | 0.0137 | 0.0154 | 0.0161 | 0.0186 | 0.0004 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0004 | 0.0004 | 0.0001 |
Cytoplasm | 0.5944 | 0.7511 | 0.7062 | 0.615 | 0.5679 | 0.7806 | 0.5623 | 0.7631 | 0.6615 | 0.6288 | 0.6132 | 0.7457 | 0.4679 | 0.7328 | 0.6142 | 0.72 | 0.4785 | 0.7156 |
Cytoplasmic Foci | 0.0203 | 0.0101 | 0.0136 | 0.0065 | 0.0144 | 0.0096 | 0.015 | 0.0051 | 0.0095 | 0.0254 | 0.0086 | 0.0086 | 0.0531 | 0.0056 | 0.0211 | 0.0072 | 0.0577 | 0.0135 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0004 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0041 | 0.0017 | 0.0018 | 0.0019 | 0.0026 | 0.0018 | 0.0143 | 0.0022 | 0.0046 | 0.0025 | 0.0019 | 0.0032 | 0.0069 | 0.0017 | 0.0045 | 0.0025 | 0.0014 | 0.0014 |
Endosome | 0.0198 | 0.0014 | 0.0054 | 0.0026 | 0.0145 | 0.0052 | 0.0223 | 0.0013 | 0.0082 | 0.0026 | 0.0048 | 0.0071 | 0.0332 | 0.0017 | 0.0093 | 0.0109 | 0.012 | 0.0048 |
Golgi | 0.0062 | 0.0003 | 0.0029 | 0.0017 | 0.0093 | 0.0014 | 0.0047 | 0.0003 | 0.005 | 0.0006 | 0.0026 | 0.0025 | 0.0114 | 0.0003 | 0.0053 | 0.0022 | 0.0073 | 0.0025 |
Lipid Particles | 0.0055 | 0.0003 | 0.0023 | 0.0002 | 0.0054 | 0.0017 | 0.0056 | 0.0001 | 0.002 | 0.0003 | 0.0008 | 0.0006 | 0.0143 | 0.0001 | 0.0028 | 0.0014 | 0.0043 | 0.0022 |
Mitochondria | 0.0049 | 0.0002 | 0.0014 | 0.0004 | 0.0088 | 0.0041 | 0.0037 | 0.0003 | 0.0029 | 0.0003 | 0.0019 | 0.0021 | 0.0052 | 0.0004 | 0.0038 | 0.0231 | 0.0014 | 0.0013 |
None | 0.2282 | 0.2214 | 0.2266 | 0.2932 | 0.2359 | 0.1664 | 0.2291 | 0.1794 | 0.2594 | 0.2727 | 0.2833 | 0.2042 | 0.2621 | 0.2248 | 0.2691 | 0.1324 | 0.283 | 0.2191 |
Nuclear Periphery | 0.012 | 0.0006 | 0.001 | 0.0006 | 0.0023 | 0.001 | 0.0369 | 0.0015 | 0.003 | 0.0011 | 0.0027 | 0.0054 | 0.0175 | 0.0008 | 0.0036 | 0.0013 | 0.0011 | 0.001 |
Nucleolus | 0.0002 | 0.0001 | 0.0008 | 0 | 0.0001 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0008 | 0.0001 | 0.0011 | 0.0004 | 0.0011 | 0.0001 |
Nucleus | 0.0018 | 0.0019 | 0.0033 | 0.0012 | 0.0014 | 0.0044 | 0.0334 | 0.0047 | 0.0042 | 0.0041 | 0.0122 | 0.0034 | 0.0095 | 0.0035 | 0.0049 | 0.0096 | 0.0033 | 0.0044 |
Peroxisomes | 0.017 | 0.0003 | 0.006 | 0.001 | 0.0501 | 0.0081 | 0.0046 | 0.0004 | 0.0028 | 0.0018 | 0.0024 | 0.0004 | 0.0251 | 0.0002 | 0.0102 | 0.0031 | 0.0387 | 0.0102 |
Punctate Nuclear | 0.0039 | 0.0018 | 0.0037 | 0.0006 | 0.0029 | 0.0032 | 0.0054 | 0.0024 | 0.0024 | 0.0028 | 0.0061 | 0.0015 | 0.0205 | 0.0013 | 0.0038 | 0.0008 | 0.0095 | 0.0022 |
Vacuole | 0.0016 | 0.0006 | 0.0007 | 0.0004 | 0.0007 | 0.0007 | 0.0045 | 0.0008 | 0.0011 | 0.0011 | 0.0012 | 0.0009 | 0.0032 | 0.0009 | 0.0017 | 0.0088 | 0.0015 | 0.0007 |
Vacuole Periphery | 0.0017 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.0027 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0004 | 0.0015 | 0.0001 | 0.0004 | 0.0011 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.8324 | 11.8193 | 19.9328 | 26.1326 | 25.7136 | 19.2208 | 20.3702 | 20.1645 | 24.6875 | 25.561 |
Translational Efficiency | 1.5628 | 1.863 | 1.1508 | 1.0805 | 1.1075 | 1.7689 | 1.1299 | 1.2248 | 1.1698 | 1.3331 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1680 | 1511 | 2927 | 2086 | 2281 | 196 | 2465 | 2066 | 3961 | 1707 | 5392 | 4152 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 670.87 | 840.71 | 921.58 | 964.13 | 750.89 | 928.51 | 919.84 | 1025.01 | 716.95 | 850.79 | 920.78 | 994.42 |
Standard Deviation | 113.43 | 104.57 | 110.22 | 133.32 | 84.97 | 113.84 | 112.40 | 501.17 | 105.73 | 109.32 | 111.22 | 367.20 |
Intensity Change Log 2 | — | 0.325575 | 0.458076 | 0.523194 | — | 0.306316 | 0.292781 | 0.448965 | — | 0.315436 | 0.373141 | 0.484467 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000083 | 0.000317 | 0.000106 | 0.000459 | 0.000064 | 0.000294 | 0.000058 | 0.000518 | 0.000072 | 0.000314 | 0.000084 | 0.000488 |
Bud Neck | 0.005173 | 0.006115 | 0.005024 | 0.005522 | 0.005307 | 0.015203 | 0.002364 | 0.004945 | 0.005250 | 0.007159 | 0.003808 | 0.005235 |
Bud Site | 0.003081 | 0.010160 | 0.010577 | 0.023102 | 0.003121 | 0.012366 | 0.004583 | 0.039537 | 0.003104 | 0.010414 | 0.007837 | 0.031280 |
Cell Periphery | 0.000112 | 0.000086 | 0.000078 | 0.000108 | 0.000055 | 0.000255 | 0.000036 | 0.000093 | 0.000079 | 0.000105 | 0.000059 | 0.000101 |
Cytoplasm | 0.576046* | 0.678487* | 0.576795* | 0.634904* | 0.663245* | 0.484645* | 0.745976* | 0.669156* | 0.626261* | 0.656230* | 0.654138* | 0.651948* |
Cytoplasmic Foci | 0.147258 | 0.121315 | 0.004080 | 0.013908 | 0.113477 | 0.269503* | 0.005775 | 0.010243 | 0.127805 | 0.138330 | 0.004855 | 0.012084 |
Eisosomes | 0.000076 | 0.000043 | 0.000010 | 0.000023 | 0.000042 | 0.000212 | 0.000007 | 0.000017 | 0.000056 | 0.000063 | 0.000009 | 0.000020 |
Endoplasmic Reticulum | 0.001017 | 0.000717 | 0.001226 | 0.001187 | 0.000620 | 0.000491 | 0.000294 | 0.000554 | 0.000788 | 0.000691 | 0.000800 | 0.000872 |
Endosome | 0.009753 | 0.008100 | 0.000546 | 0.003587 | 0.003993 | 0.023322 | 0.000287 | 0.003076 | 0.006436 | 0.009848 | 0.000427 | 0.003333 |
Golgi | 0.001173 | 0.001742 | 0.000065 | 0.001002 | 0.000778 | 0.003657 | 0.000046 | 0.001696 | 0.000946 | 0.001962 | 0.000056 | 0.001347 |
Lipid Particles | 0.005525 | 0.001854 | 0.000273 | 0.000783 | 0.002149 | 0.003632 | 0.000194 | 0.000466 | 0.003581 | 0.002058 | 0.000237 | 0.000625 |
Mitochondria | 0.002737 | 0.001473 | 0.000632 | 0.001998 | 0.000853 | 0.013636 | 0.000266 | 0.001368 | 0.001652 | 0.002870 | 0.000465 | 0.001684 |
Mitotic Spindle | 0.002130 | 0.002922 | 0.002775 | 0.053718 | 0.001071 | 0.000673 | 0.003629 | 0.050721 | 0.001520 | 0.002664 | 0.003165 | 0.052227 |
None | 0.012253 | 0.003782 | 0.005285 | 0.005580 | 0.003861 | 0.000885 | 0.005181 | 0.010301 | 0.007420 | 0.003450 | 0.005238 | 0.007929 |
Nuclear Periphery | 0.000485 | 0.000202 | 0.000903 | 0.001243 | 0.000198 | 0.000268 | 0.000489 | 0.000551 | 0.000320 | 0.000209 | 0.000714 | 0.000899 |
Nuclear Periphery Foci | 0.001065 | 0.000926 | 0.000342 | 0.001887 | 0.000382 | 0.000800 | 0.000235 | 0.000953 | 0.000672 | 0.000911 | 0.000293 | 0.001423 |
Nucleolus | 0.002240 | 0.000840 | 0.000128 | 0.000465 | 0.000446 | 0.000516 | 0.000089 | 0.000298 | 0.001207 | 0.000803 | 0.000110 | 0.000382 |
Nucleus | 0.162081* | 0.095457 | 0.366439* | 0.128519 | 0.153180* | 0.086870 | 0.197396* | 0.119597 | 0.156955* | 0.094471 | 0.289159* | 0.124080 |
Peroxisomes | 0.001549 | 0.003040 | 0.000085 | 0.001092 | 0.000972 | 0.005589 | 0.000174 | 0.000524 | 0.001217 | 0.003333 | 0.000125 | 0.000809 |
Vacuole | 0.065259 | 0.061467 | 0.024402 | 0.120271 | 0.045769 | 0.074395 | 0.032881 | 0.083684 | 0.054035 | 0.062952 | 0.028278 | 0.102066 |
Vacuole Periphery | 0.000903 | 0.000955 | 0.000231 | 0.000644 | 0.000419 | 0.002787 | 0.000040 | 0.001700 | 0.000624 | 0.001166 | 0.000144 | 0.001170 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.73 | -3.08 | -5.90 | 0.99 | -4.09 | -3.94 | -2.46 | -4.91 | 0.03 | -4.06 | -4.31 | -4.28 | -7.46 | 0.86 | -5.61 |
Bud Neck | -1.43 | 0.64 | 3.38 | 3.96 | 1.88 | -2.58 | 5.21 | 3.40 | 3.04 | -2.39 | -2.65 | 3.43 | 4.72 | 5.11 | 0.43 |
Bud Site | -5.72 | -4.37 | -5.32 | 0.36 | -0.83 | -3.09 | -0.46 | -7.61 | -0.90 | -7.06 | -6.48 | -4.08 | -9.26 | -1.36 | -5.39 |
Cell Periphery | 3.71 | 3.84 | 5.37 | 2.64 | 0.44 | -6.04 | 5.68 | -2.46 | 5.76 | -6.57 | -4.42 | 4.06 | 3.17 | 6.44 | -1.53 |
Cytoplasm | -10.79 | 6.29 | 7.06 | 18.10 | 1.60 | 9.18 | 1.36 | 16.12 | -3.03 | 14.91 | -3.39 | 8.05 | 17.10 | 16.28 | 10.29 |
Cytoplasmic Foci | 4.59 | 36.56 | 34.89 | 28.31 | -8.98 | -10.63 | 33.95 | 33.27 | 18.23 | -3.27 | -2.32 | 49.59 | 47.77 | 32.27 | -9.16 |
Eisosomes | 6.72 | 16.32 | 12.95 | 5.70 | -13.32 | -5.84 | 13.58 | 9.23 | 6.55 | -10.88 | -1.40 | 20.89 | 15.55 | 8.81 | -16.88 |
Endoplasmic Reticulum | 2.31 | -2.25 | -1.14 | -4.34 | 1.62 | 1.07 | 1.17 | -1.91 | -3.01 | -6.17 | 0.97 | -2.21 | -2.40 | -4.14 | 0.72 |
Endosome | 1.81 | 13.33 | 11.62 | 10.06 | -4.87 | -4.88 | 12.94 | 7.74 | 5.57 | -4.72 | -4.24 | 17.68 | 13.86 | 11.40 | -6.69 |
Golgi | -1.54 | 6.40 | 4.50 | 4.24 | -2.65 | -2.43 | 11.30 | 1.53 | 2.69 | -1.79 | -3.05 | 10.78 | 4.08 | 4.66 | -2.66 |
Lipid Particles | 8.52 | 13.34 | 11.95 | 5.15 | -12.46 | -2.60 | 4.63 | 3.64 | 6.65 | -11.92 | 4.59 | 11.58 | 9.94 | 7.22 | -16.96 |
Mitochondria | 2.05 | 3.73 | 3.19 | 1.73 | -1.99 | -1.93 | 6.33 | 0.97 | 1.95 | -3.50 | -1.42 | 4.70 | 3.27 | 2.52 | -3.57 |
Mitotic Spindle | -0.56 | -1.60 | -8.57 | -8.04 | -8.21 | 0.68 | -3.36 | -9.64 | -9.98 | -7.98 | -1.07 | -3.75 | -12.96 | -11.20 | -11.49 |
None | 5.60 | 4.93 | 4.67 | -2.74 | -0.78 | 7.21 | -5.26 | -5.98 | -10.68 | -3.59 | 4.77 | 2.97 | 0.84 | -5.14 | -3.57 |
Nuclear Periphery | 7.35 | -15.96 | -15.14 | -20.56 | -5.90 | -1.39 | -21.36 | -28.49 | -18.73 | -9.66 | 5.06 | -27.56 | -27.39 | -31.26 | -9.78 |
Nuclear Periphery Foci | 0.56 | 6.29 | -0.36 | -0.66 | -3.12 | -2.53 | 0.99 | -7.40 | -1.00 | -8.81 | -3.16 | 5.28 | -3.24 | -0.68 | -5.71 |
Nucleolus | 5.22 | 10.72 | 9.65 | 3.40 | -5.46 | -1.13 | 8.73 | 2.88 | 3.14 | -6.12 | 2.00 | 12.24 | 9.91 | 3.63 | -8.07 |
Nucleus | 12.29 | -37.50 | -4.19 | -19.10 | 36.63 | 7.63 | -19.50 | -8.51 | -11.93 | 9.62 | 16.40 | -42.50 | -9.09 | -24.75 | 33.65 |
Peroxisomes | -3.61 | 12.60 | 7.49 | 6.61 | -2.20 | -4.57 | 7.54 | 7.57 | 5.36 | -0.80 | -5.63 | 14.26 | 9.97 | 8.22 | -2.38 |
Vacuole | 0.26 | 8.25 | -27.72 | -27.51 | -33.27 | -4.12 | -8.60 | -27.60 | -13.26 | -21.04 | -4.18 | -0.50 | -39.51 | -32.48 | -38.85 |
Vacuole Periphery | -0.27 | 6.87 | 8.58 | 5.26 | -0.46 | -1.94 | 12.21 | -0.13 | 1.90 | -2.37 | -2.84 | 9.28 | 3.14 | 4.07 | -2.23 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|