Standard name
Human Ortholog
Description Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.42 0.51 0.46 0.31 0.19 0.2 0.16 0.13 0.12 0.07 0.1 0.45 0.38 0.27 0.08 0.06 0.07 0.29 0.19 0.19 0.39 0.3 0.33
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Endosome 0.19 0.36 0.06 0.1 0.11 0.05 0 0.06 0 0 0 0 0.34 0.45 0.56 0.45 0.34 0.26 0.26 0.35 0.29 0.21 0.25 0.19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.11 0.17 0.15 0.32 0.58 0.66 0.61 0.71 0.77 0.75 0.8 0.05 0.06 0 0.14 0.1 0.07 0.11 0.09 0.13 0.08 0.12 0.1
Nucleus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0.06 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.1 0.09 0.12 0.16 0.18 0.21 0.3 0 0.06 0.07 0.26 0.28 0.34 0 0 0 0 0 0
Peroxisomes 0.12 0.17 0 0.13 0.14 0.1 0.09 0.11 0 0 0.06 0 0.18 0.29 0.35 0.31 0.27 0.28 0 0.05 0 0 0.06 0
SpindlePole 0.36 0.23 0.29 0.29 0.29 0.26 0.18 0.16 0.1 0.12 0.15 0.07 0.24 0.23 0.26 0.59 0.56 0.49 0.14 0.21 0.25 0.19 0.19 0.19
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 8 6 5 8 8
Bud 4 3 12 13 6 17 18 21 13 14 4 3 6 2 0 0 0 1 4 11 4 2 5 8
Bud Neck 2 2 13 2 1 2 5 0 1 1 0 0 1 2 0 1 0 2 1 2 1 0 3 5
Bud Site 0 1 1 1 0 5 11 9 7 8 1 5 0 0 0 1 0 3 – – – – – –
Cell Periphery 2 4 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Cytoplasm 38 175 158 95 58 73 81 82 39 38 8 16 274 176 139 26 20 15 73 88 61 101 130 125
Endoplasmic Reticulum 0 2 0 1 3 1 0 0 0 0 0 0 0 0 1 4 4 5 13 0 3 0 2 1
Endosome 22 148 18 20 21 20 16 30 9 5 3 2 208 211 283 150 116 58 64 167 92 55 106 73
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 5 1 1 1 1
Mitochondria 15 44 53 31 60 225 264 318 217 244 82 130 31 28 19 46 33 15 27 42 42 21 49 40
Nucleus 6 0 0 0 1 2 6 8 5 1 2 3 1 3 2 3 5 3 0 0 0 1 0 1
Nuclear Periphery 0 3 1 0 0 2 6 15 18 5 7 12 6 3 4 0 6 0 0 2 0 0 0 0
Nucleolus 4 5 8 3 4 38 37 64 50 57 23 48 25 29 37 87 94 75 0 2 1 0 1 1
Peroxisomes 14 69 13 26 26 39 37 55 13 14 6 4 110 136 180 102 92 62 6 21 11 8 26 10
SpindlePole 42 97 89 60 55 100 71 83 31 37 16 11 149 107 134 197 189 108 34 101 82 48 80 71
Vac/Vac Membrane 1 16 0 0 3 3 6 1 6 5 3 3 8 15 15 11 16 9 8 8 5 3 4 21
Unique Cell Count 116 415 309 205 190 390 402 523 304 318 109 162 610 465 508 333 340 219 253 476 322 262 430 385
Labelled Cell Count 150 569 367 252 238 527 559 686 409 429 155 237 820 712 814 628 576 357 253 476 322 262 430 385


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.6 3.5 3.3 3.6 3.5 3.3 3.8 4.0 4.0 4.5 4.5 4.7 4.8 5.3 7.7 9.6 8.8 3.7 3.7 4.6
Std Deviation (1e-4) 1.0 1.2 1.2 0.7 1.2 1.2 1.2 1.3 1.6 1.5 1.5 1.6 1.3 1.5 2.1 3.0 3.3 3.0 1.1 1.0 1.2
Intensity Change (Log2) – – – -0.12 0.03 -0.03 -0.12 0.11 0.17 0.17 0.33 0.34 0.4 0.45 0.58 1.13 1.43 1.32 0.05 0.08 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 3.2 3.9 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4779 3.8718 3.4236 3.7128 3.4433 3.7953 2.4962 2.4626 2.3782 1.4923 2.2872 1.9853 3.6108 2.7692 2.9841 3.4348 4.5131 2.9904
Actin 0.0068 0.0157 0.0126 0.002 0.0011 0.0005 0.0341 0.034 0.0005 0.0687 0.0012 0.0003 0.0196 0.0003 0.0143 0.0038 0.015 0.0032
Bud 0.0036 0.0018 0.001 0.0032 0.0003 0.0002 0.0004 0.0002 0.0005 0.0463 0.0001 0 0.0002 0.0015 0.0007 0.0009 0.0002 0.0001
Bud Neck 0.0018 0.003 0.0009 0.0015 0.0016 0.0028 0.0007 0.0034 0.0007 0.0002 0.0003 0.0006 0.0004 0.0014 0.0027 0.0018 0.0003 0.0012
Bud Periphery 0.0046 0.002 0.0007 0.0037 0.001 0.0002 0.0004 0.0002 0.0006 0.0916 0 0 0.0004 0.0014 0.001 0.0015 0.0004 0.0002
Bud Site 0.0108 0.0164 0.0141 0.0077 0.001 0.0004 0.0013 0.0089 0.0015 0.0028 0.0001 0.0001 0.0004 0.0102 0.0075 0.0028 0.0006 0.0004
Cell Periphery 0.0005 0.0007 0.0002 0.0004 0.0007 0.0006 0.0002 0.0003 0.0003 0.0005 0 0 0.0001 0.0005 0.0003 0.0029 0.0001 0.0001
Cytoplasm 0.0212 0.019 0.0067 0.0069 0.0071 0.0364 0.0163 0.0194 0.0316 0.0072 0.0009 0.0365 0.024 0.0271 0.0157 0.0205 0.0081 0.0248
Cytoplasmic Foci 0.275 0.239 0.2087 0.3077 0.2744 0.2057 0.3359 0.3156 0.1865 0.2881 0.3537 0.2484 0.1794 0.2707 0.1946 0.1414 0.246 0.2399
Eisosomes 0.0002 0.0007 0.0003 0.0001 0.0002 0 0.0004 0.0003 0.0002 0.0002 0 0 0.0005 0.0001 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0021 0.0021 0.0011 0.0003 0.0013 0.0009 0.0049 0.0023 0.0021 0.0012 0.0003 0.0024 0.003 0.0017 0.0014 0.0025 0.0018 0.0005
Endosome 0.0662 0.0813 0.0718 0.0425 0.1306 0.0592 0.1328 0.088 0.0745 0.1073 0.0837 0.1023 0.0724 0.1079 0.0683 0.1405 0.0439 0.0535
Golgi 0.0255 0.0114 0.0233 0.0091 0.0158 0.0115 0.0706 0.0211 0.0163 0.026 0.1331 0.0063 0.0197 0.0172 0.0185 0.0306 0.0097 0.0109
Lipid Particles 0.34 0.3117 0.287 0.3327 0.334 0.4666 0.1608 0.192 0.2871 0.1872 0.366 0.3865 0.4307 0.299 0.3528 0.2285 0.2704 0.4185
Mitochondria 0.0126 0.0041 0.0099 0.0424 0.0371 0.0087 0.0063 0.0021 0.0107 0.0043 0.0034 0.0003 0.0179 0.0064 0.0037 0.0189 0.0483 0.002
None 0.0541 0.1138 0.1461 0.1122 0.1097 0.0779 0.008 0.1699 0.2585 0.1377 0.0001 0.0968 0.0874 0.0817 0.1624 0.2896 0.2248 0.132
Nuclear Periphery 0.0054 0.0056 0.002 0.0004 0.0041 0.0013 0.0273 0.0022 0.0023 0.0011 0.0001 0.0021 0.0355 0.0027 0.0018 0.0044 0.0257 0.0013
Nucleolus 0.0153 0.0228 0.0211 0.0067 0.0039 0.0135 0.0024 0.0305 0.0104 0.0004 0.0001 0.0167 0.001 0.029 0.04 0.0017 0.0005 0.0073
Nucleus 0.0154 0.0076 0.0035 0.0007 0.0018 0.0014 0.1067 0.0028 0.0057 0.0003 0 0.0011 0.0167 0.0093 0.0025 0.0015 0.0187 0.0018
Peroxisomes 0.0806 0.0508 0.1108 0.095 0.0402 0.0587 0.0209 0.0689 0.0432 0.0176 0.0548 0.0652 0.0467 0.0516 0.0701 0.0543 0.0388 0.0476
Punctate Nuclear 0.048 0.0543 0.074 0.0213 0.0138 0.0406 0.065 0.0321 0.0595 0.0094 0.0013 0.0299 0.0371 0.0484 0.0375 0.0063 0.044 0.0506
Vacuole 0.0081 0.0313 0.0033 0.0027 0.0127 0.011 0.0028 0.0049 0.0064 0.0015 0.0004 0.0039 0.003 0.0292 0.0031 0.0409 0.0017 0.0034
Vacuole Periphery 0.0021 0.0048 0.001 0.0008 0.0077 0.0021 0.0018 0.0008 0.0011 0.0004 0.0004 0.0005 0.0037 0.003 0.0008 0.0045 0.0011 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.1378 25.1535 16.8874 23.8047 16.938 18.0786 22.7077 27.3828 24.4271 26.2147
Translational Efficiency 0.5951 0.5866 0.82 0.5023 0.6729 0.9137 0.6876 0.4833 0.7453 0.5754

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2056 1552 296 1453 1210 1942 479 976 3266 3494 775 2429

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 627.13 702.55 981.53 831.67 665.81 694.92 903.67 1147.45 641.46 698.31 933.41 958.55
Standard Deviation 64.59 90.04 178.22 147.57 73.52 94.69 131.09 193.71 70.55 92.73 155.51 228.19
Intensity Change Log 2 — 0.163836 0.646268 0.407247 — 0.061736 0.440685 0.785249 — 0.112162 0.544062 0.614204

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000300 0.000581 0.004502 0.009095 0.000567 0.000228 0.003077 0.002258 0.000399 0.000385 0.003621 0.006348
Bud Neck 0.032796 0.027940 0.027462 0.016765 0.021241 0.045353 0.007540 0.025085 0.028515 0.037618 0.015149 0.020108
Bud Site 0.006464 0.013799 0.011974 0.046279 0.004046 0.014630 0.008693 0.037501 0.005568 0.014261 0.009946 0.042752
Cell Periphery 0.000231 0.000110 0.000161 0.000169 0.000341 0.000149 0.000121 0.000104 0.000272 0.000132 0.000136 0.000143
Cytoplasm 0.324270* 0.217573* 0.002945 0.056570 0.263718* 0.203740* 0.018617 0.085930 0.301836* 0.209884* 0.012631 0.068368
Cytoplasmic Foci 0.338045* 0.320224* 0.193462* 0.165300 0.264045* 0.289223* 0.146487 0.148796 0.310629* 0.302993* 0.164429 0.158668
Eisosomes 0.000309 0.000115 0.000375 0.000066 0.000204 0.000124 0.000088 0.000028 0.000270 0.000120 0.000197 0.000051
Endoplasmic Reticulum 0.000801 0.001158 0.001321 0.001161 0.001213 0.000747 0.001626 0.000332 0.000953 0.000929 0.001510 0.000828
Endosome 0.040186 0.091287 0.088334 0.131718 0.051728 0.121665 0.127668 0.159606 0.044462 0.108172 0.112645 0.142923
Golgi 0.013294 0.022754 0.018175 0.026707 0.009737 0.018554 0.011658 0.023601 0.011976 0.020420 0.014147 0.025459
Lipid Particles 0.046539 0.032924 0.247660* 0.048137 0.101950* 0.036648 0.169832* 0.021659 0.067068 0.034994 0.199557* 0.037498
Mitochondria 0.009454 0.024093 0.002089 0.010343 0.009580 0.030419 0.001754 0.027545 0.009501 0.027609 0.001882 0.017255
Mitotic Spindle 0.004441 0.043705 0.170241* 0.300471* 0.012938 0.035022 0.259389* 0.333513* 0.007589 0.038879 0.225340* 0.313748*
None 0.007923 0.002830 0.000224 0.000731 0.008822 0.004122 0.001573 0.002085 0.008256 0.003548 0.001057 0.001275
Nuclear Periphery 0.000409 0.000835 0.006555 0.002904 0.001314 0.001054 0.003992 0.001273 0.000744 0.000956 0.004971 0.002249
Nuclear Periphery Foci 0.001715 0.003113 0.090200 0.012499 0.004614 0.003481 0.063984 0.005129 0.002789 0.003318 0.073997 0.009538
Nucleolus 0.004124 0.004887 0.011904 0.002990 0.011182 0.009415 0.014132 0.004117 0.006739 0.007404 0.013281 0.003443
Nucleus 0.101787 0.074538 0.009763 0.016497 0.160513* 0.068090 0.031277 0.007142 0.123544 0.070954 0.023060 0.012738
Peroxisomes 0.030753 0.048814 0.095809 0.096484 0.044706 0.037011 0.097555 0.070714 0.035922 0.042254 0.096888 0.086129
Vacuole 0.035192 0.065392 0.016028 0.051343 0.026226 0.075524 0.029667 0.039791 0.031870 0.071024 0.024458 0.046701
Vacuole Periphery 0.000968 0.003327 0.000816 0.003770 0.001315 0.004802 0.001272 0.003791 0.001097 0.004147 0.001098 0.003778

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.40 -2.49 -7.16 -6.86 -1.41 0.90 -3.31 -2.13 -3.03 0.96 0.07 -4.08 -7.39 -7.57 -1.73
Bud Neck 1.99 1.42 6.06 3.77 2.41 -7.58 4.64 -2.95 4.45 -8.14 -4.39 6.10 2.49 6.93 -3.90
Bud Site -5.54 -3.04 -12.79 -9.83 -8.71 -6.45 -3.68 -11.53 -7.16 -7.15 -8.15 -4.42 -16.90 -12.00 -11.31
Cell Periphery 4.78 1.59 2.13 -3.84 0.29 4.98 5.37 6.71 2.83 2.72 6.33 5.04 5.85 -0.77 0.85
Cytoplasm 11.77 51.83 30.57 15.96 -18.19 5.89 24.76 14.58 10.56 -8.94 13.98 48.67 32.69 19.36 -15.05
Cytoplasmic Foci 2.59 12.80 27.76 24.16 3.89 -2.29 13.22 15.76 21.21 0.50 1.87 20.92 31.66 31.99 2.65
Eisosomes 6.33 -0.95 7.69 7.46 4.52 7.02 6.99 18.29 15.34 8.14 7.57 1.76 10.99 14.45 5.81
Endoplasmic Reticulum -1.96 -3.50 -5.87 -2.74 0.07 1.37 -2.57 2.48 4.99 6.88 0.06 -4.81 -1.67 -2.37 4.15
Endosome -11.50 -5.99 -14.58 -3.42 -2.51 -12.91 -7.66 -12.77 -2.94 -2.52 -18.54 -10.31 -19.44 -3.48 -2.53
Golgi -4.56 -2.02 -3.19 1.18 -0.79 -5.00 -0.08 -5.06 -0.66 -5.16 -6.27 -0.61 -5.43 0.31 -4.53
Lipid Particles 4.01 -11.21 2.25 -1.51 11.74 10.65 -3.35 13.56 5.83 11.63 10.14 -11.50 10.97 1.92 15.77
Mitochondria -6.65 7.49 0.79 6.67 -4.68 -8.16 4.64 -4.57 1.69 -8.08 -11.06 8.83 -3.22 6.46 -9.32
Mitotic Spindle -11.13 -10.57 -30.17* -24.12* -5.89 -6.20 -15.04 -27.65* -25.23* -4.58 -13.10 -18.56 -40.87 -34.89* -6.43
None 4.63 7.36 6.55 4.86 -5.71 4.09 5.04 5.93 3.39 0.53 5.75 7.59 8.72 5.89 0.36
Nuclear Periphery -4.36 -7.30 -6.11 -5.28 2.91 1.20 -5.14 -2.45 -3.60 3.67 -2.47 -9.21 -6.67 -6.06 3.71
Nuclear Periphery Foci -2.49 -9.06 -7.90 -6.35 7.86 1.04 -8.31 0.23 -0.79 8.33 -1.19 -12.32 -7.18 -5.88 11.06
Nucleolus -1.22 -3.49 0.02 1.06 3.45 1.52 -1.79 4.01 3.33 3.77 -0.55 -4.06 3.14 3.79 5.09
Nucleus 5.15 15.01 17.02 9.37 -3.21 12.84 11.39 21.74 15.51 4.73 13.02 15.14 27.39 15.80 2.10
Peroxisomes -5.60 -7.01 -9.93 -5.68 1.84 2.18 -4.90 -2.88 -4.76 2.78 -2.81 -8.70 -9.68 -8.03 2.87
Vacuole -9.54 5.45 -13.33 -6.42 -15.54 -14.72 -4.44 -8.19 2.35 -1.09 -16.66 -2.35 -15.44 -4.07 -8.78
Vacuole Periphery -7.65 1.29 -4.24 0.63 -4.48 -7.53 0.22 -3.77 2.63 -3.83 -11.77 0.13 -5.75 2.55 -5.17
Standard name
Human Ortholog
Description Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Tgl1

Tgl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tgl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available