Standard name
Human Ortholog
Description Lysine methyltransferase; involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm


Mutation Strain ID Temperature Mean Single Mutant Fitness Single Mutant Fitness SD Penetrance Results Specific Phenotype Results
efm1Δ DMA2070 30 1.0252 0.0667
compartment (FP-tagged protein)# cells# replicatesmean penetrancepenetrance SDpenetrance mutantimage
Actin patch (Sac6)244114.8no
Cortical patch (Sla1)258114.0no
Late endosome (Snf7)120134.2no
Vacuole (Vph1)78125.6no
phenotypemeanSDSPMdiagram
Actin patchActin patch
Actin patch (Sac6)aggregate0no
Actin patch (Sac6)bright patches1.6no
Actin patch (Sac6)decreased patch number4.5no
Actin patch (Sac6)depolarized patches8.6no
Actin patch (Sac6)WT85.3NANA
Cortical patchCortical patch
Cortical patch (Sla1)aggregate2.7no
Cortical patch (Sla1)decreased patch number7.8no
Cortical patch (Sla1)depolarized patches2.3no
Cortical patch (Sla1)increased patch number0.8no
Cortical patch (Sla1)WT86.0NANA
Late endosomeLate endosome
Late endosome (Snf7)fragmented1.7no
Late endosome (Snf7)condensed23.3no
Late endosome (Snf7)membrane **5.0no
Late endosome (Snf7)WT65.8NANA
VacuoleVacuole
Vacuole (Vph1)class E3.8no
Vacuole (Vph1)enlarged2.6no
Vacuole (Vph1)fragmented9.0no
Vacuole (Vph1)multilobed5.1no
Vacuole (Vph1)class G2.6no
Vacuole (Vph1)V-ATPase defect2.6no
Vacuole (Vph1)WT74.3NANA
penetrance = percentage of the population with an aberrant phenotype

FP = fluorescent protein

SD = standard deviation

* temperature-sentitive strains imaged at room temperature (RT) were analyzed but not scored

specific phenotype mutant (SPM)
  • yes+ = stringent SPM

** membrane = localization of Snf7 to the vacuolar membrane

NA = not applicable

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.95 0.93 0.87 0.83 0.79 0.62 0.7 0.58 0.6 0.99 0.97 0.91 0.92 0.92 0.89 0.95 0.63 0.87 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.06 0.06 0.24 0.25 0.43 0.36 0 0 0 0 0 0 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.1 0.11 0.19 0.13 0.07 0.1 0 0 0.07 0.06 0.08 0 0 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 3 1 1 3 1 0 1 3 0 0 2 0 2 2 0 0 0 0 0
Bud 0 0 0 0 0 2 0 3 2 3 2 0 0 0 0 0 1 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 3 4 7 4 5 7 3 4 1 7 2 3 17 20 20 0 0 0 0 0 0
Cytoplasm 161 233 103 246 211 237 271 146 167 113 166 67 139 310 330 316 155 226 32 20 61 84
Endoplasmic Reticulum 2 3 0 3 11 12 17 3 0 6 4 0 1 10 14 13 1 1 2 0 0 1
Endosome 1 0 1 2 1 4 6 4 5 0 2 0 0 1 1 3 1 1 0 0 0 1
Golgi 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0
Mitochondria 3 0 0 2 14 18 21 57 60 85 100 0 0 0 3 3 3 0 5 0 0 0
Nucleus 2 0 1 2 4 2 6 5 5 3 5 1 3 3 2 2 1 2 2 0 0 1
Nuclear Periphery 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 0 1 1 1 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 2 3 8 11 29 36 44 31 14 28 0 1 25 23 27 1 3 4 0 0 0
Unique Cell Count 169 237 108 264 243 285 341 237 240 196 276 68 143 340 359 344 174 239 52 23 67 93
Labelled Cell Count 171 241 113 274 257 311 369 266 275 229 318 70 147 369 394 389 174 239 52 23 67 93


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 10.7 9.4 9.3 7.5 7.3 7.4 6.1 6.0 5.5 5.5 8.7 8.5 12.6 12.9 11.8 8.1 10.0
Std Deviation (1e-4) 1.2 1.6 1.6 1.5 1.3 1.7 1.6 1.3 1.0 1.1 1.0 1.7 1.3 2.4 2.4 2.3 1.4 1.7
Intensity Change (Log2)

RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015HU120HU160051015rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7275 3.6258 3.3064 2.812 2.7118 3.022 2.5843 4.4851 3.685 3.1365 2.7241 3.9638 1.6508 2.7723 2.4848 2.1489 1.8355 2.2828
Actin 0.0139 0.0077 0.0039 0.0085 0.0114 0.0063 0.0095 0.0033 0.0023 0.0204 0.0336 0.0024 0.0363 0.0004 0.0033 0.0538 0.0115 0.0021
Bud 0.0074 0.0151 0.001 0.0176 0.0003 0.0041 0.0002 0.0003 0.0004 0.0025 0.0022 0.0002 0.0005 0.0003 0.0029 0.0008 0.0015 0.0002
Bud Neck 0.0015 0.0005 0.001 0.0005 0.0014 0.002 0.0015 0.0004 0.0003 0.0018 0.0011 0.0012 0.0026 0.0001 0.0008 0.007 0.0011 0.0006
Bud Periphery 0.0077 0.0047 0.001 0.0116 0.0002 0.0021 0.0002 0.0001 0.0005 0.001 0.0037 0.0001 0.0004 0.0001 0.0013 0.0008 0.0021 0.0002
Bud Site 0.0073 0.0172 0.014 0.0052 0.0005 0.0012 0.0038 0.0064 0.0005 0.0008 0.0034 0.0001 0.0024 0.0004 0.0021 0.0061 0.0038 0.0003
Cell Periphery 0.0006 0.0006 0.0004 0.0006 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0
Cytoplasm 0.6762 0.8538 0.8696 0.7818 0.7253 0.8679 0.8396 0.9305 0.9135 0.8689 0.5766 0.8817 0.7931 0.9396 0.9008 0.8472 0.8459 0.9059
Cytoplasmic Foci 0.0282 0.0155 0.0235 0.0141 0.032 0.0249 0.0269 0.0095 0.0203 0.0396 0.0606 0.0237 0.0282 0.0076 0.0137 0.0225 0.0188 0.0174
Eisosomes 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0002 0 0.0004 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.0128 0.0041 0.0048 0.0103 0.011 0.0063 0.0075 0.0016 0.0038 0.0036 0.0014 0.0045 0.0076 0.0017 0.0038 0.0055 0.0053 0.0026
Endosome 0.0767 0.0125 0.0158 0.016 0.087 0.0223 0.0382 0.0031 0.0319 0.0162 0.0887 0.0314 0.025 0.006 0.0143 0.0221 0.0334 0.0183
Golgi 0.0132 0.0021 0.0015 0.002 0.0063 0.0031 0.0046 0.0008 0.0037 0.0101 0.0371 0.0177 0.0036 0.0008 0.0014 0.0054 0.0052 0.0028
Lipid Particles 0.0144 0.0011 0.001 0.0003 0.0072 0.0015 0.0079 0.0001 0.0017 0.0001 0.0169 0.0038 0.0086 0 0.0005 0.0025 0.0041 0.0075
Mitochondria 0.0073 0.0025 0.0061 0.0263 0.0023 0.0015 0.0011 0.0004 0.0014 0.0056 0.0476 0.0196 0.0021 0.0007 0.0078 0.0014 0.0023 0.0012
None 0.0856 0.0529 0.0446 0.0708 0.0813 0.0469 0.0343 0.0409 0.0116 0.0048 0.0012 0.0054 0.0549 0.0392 0.0362 0.0113 0.0343 0.0151
Nuclear Periphery 0.0147 0.0013 0.001 0.0088 0.0129 0.0021 0.0019 0.0003 0.0008 0.0012 0.0012 0.0004 0.0104 0.0004 0.0013 0.0028 0.0062 0.0058
Nucleolus 0.0008 0.0003 0.0002 0.001 0.0005 0.0001 0.0006 0 0 0 0.0003 0 0.0009 0 0.0009 0.0002 0.0013 0.0006
Nucleus 0.006 0.0027 0.0018 0.0139 0.0052 0.003 0.0016 0.0007 0.0008 0.0018 0.0013 0.0008 0.0045 0.0007 0.0036 0.002 0.0057 0.0024
Peroxisomes 0.0042 0.0005 0.0016 0.0014 0.0015 0.0005 0.0043 0.0003 0.0033 0.0189 0.0893 0.0045 0.0059 0.0001 0.0006 0.0021 0.0017 0.0119
Punctate Nuclear 0.0058 0.0017 0.0026 0.0042 0.007 0.0011 0.0083 0.0001 0.0008 0.0003 0.0008 0.0002 0.0085 0.0002 0.0021 0.0027 0.0115 0.0028
Vacuole 0.0105 0.0028 0.0039 0.0035 0.0045 0.0024 0.0068 0.0008 0.0016 0.0019 0.0234 0.0014 0.0032 0.0014 0.0018 0.0027 0.0031 0.0014
Vacuole Periphery 0.0048 0.0004 0.0007 0.0015 0.0021 0.0007 0.0008 0.0001 0.0006 0.0005 0.0091 0.0008 0.0008 0.0002 0.0004 0.0007 0.0009 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.0362 25.8096 29.9768 32.9873 32.9578 37.0107 22.6922 20.6762 22.585 32.4679
Translational Efficiency 0.7992 1.0062 0.9301 0.7172 0.7903 0.8103 0.8776 0.9226 0.7778 0.7752

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1854 945 1435 1583 1018 1644 1092 2486 2872 2589 2527 4069

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 798.36 920.79 1147.92 1157.79 911.12 989.68 1151.11 1199.97 838.33 964.53 1149.30 1183.56
Standard Deviation 106.62 170.81 161.19 156.74 128.32 151.37 138.82 156.56 126.82 162.17 151.93 157.97
Intensity Change Log 2 0.205833 0.523911 0.536262 0.119321 0.337313 0.397285 0.160370 0.427468 0.463859

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000312 0.001057 0.000410 0.000563 0.000544 0.000469 0.000372 0.001099 0.000394 0.000683 0.000394 0.000890
Bud Neck 0.019092 0.007757 0.001796 0.002494 0.005632 0.005701 0.001958 0.003096 0.014321 0.006451 0.001866 0.002862
Bud Site 0.039261 0.075302 0.010491 0.032306 0.032359 0.041759 0.007179 0.019415 0.036815 0.054002 0.009060 0.024430
Cell Periphery 0.000109 0.000095 0.000082 0.000097 0.000143 0.000072 0.000078 0.000038 0.000121 0.000081 0.000080 0.000061
Cytoplasm 0.457702 0.551504 0.808662 0.784762 0.489038 0.517137 0.694298 0.782414 0.468810 0.529682 0.759241 0.783327
Cytoplasmic Foci 0.275835 0.197190 0.024106 0.047593 0.326372 0.235642 0.052599 0.031270 0.293748 0.221606 0.036419 0.037620
Eisosomes 0.000054 0.000028 0.000007 0.000007 0.000049 0.000023 0.000017 0.000008 0.000052 0.000025 0.000011 0.000008
Endoplasmic Reticulum 0.002715 0.001132 0.001275 0.000267 0.003027 0.001508 0.001696 0.000298 0.002826 0.001371 0.001457 0.000286
Endosome 0.037067 0.031186 0.002782 0.004503 0.031230 0.033244 0.008660 0.004739 0.034998 0.032493 0.005322 0.004647
Golgi 0.019155 0.010898 0.000636 0.005782 0.018104 0.016474 0.001465 0.004335 0.018782 0.014439 0.000994 0.004898
Lipid Particles 0.003512 0.001656 0.000747 0.000848 0.003126 0.001388 0.001552 0.000484 0.003375 0.001486 0.001095 0.000626
Mitochondria 0.017184 0.004874 0.000190 0.001301 0.002707 0.007080 0.000181 0.004263 0.012052 0.006275 0.000186 0.003111
Mitotic Spindle 0.004786 0.006038 0.007699 0.020513 0.002547 0.015111 0.006815 0.037738 0.003993 0.011799 0.007317 0.031037
None 0.002733 0.000660 0.002596 0.002094 0.000749 0.001003 0.002287 0.001330 0.002030 0.000878 0.002463 0.001627
Nuclear Periphery 0.000444 0.000134 0.000315 0.000131 0.000112 0.000116 0.000538 0.000166 0.000327 0.000123 0.000411 0.000152
Nuclear Periphery Foci 0.000503 0.001325 0.003345 0.001169 0.001198 0.000506 0.004300 0.000497 0.000750 0.000805 0.003758 0.000759
Nucleolus 0.000932 0.000396 0.000171 0.000192 0.000301 0.000468 0.000399 0.000290 0.000708 0.000441 0.000269 0.000252
Nucleus 0.022821 0.009819 0.100882 0.022481 0.015638 0.007273 0.057614 0.029992 0.020275 0.008202 0.082185 0.027070
Peroxisomes 0.005321 0.007629 0.000435 0.001260 0.003739 0.005216 0.000579 0.002342 0.004760 0.006097 0.000497 0.001921
Vacuole 0.078729 0.083216 0.032959 0.068509 0.056237 0.100529 0.155660 0.072404 0.070757 0.094210 0.085982 0.070889
Vacuole Periphery 0.011732 0.008105 0.000412 0.003127 0.007149 0.009280 0.001754 0.003781 0.010108 0.008851 0.000992 0.003527

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.98 -4.62 -2.57 4.52 0.96 1.12 1.63 -0.98 -1.46 -1.74 -5.08 -2.03 -2.18 0.77 -1.29
Bud Neck 8.38 13.57 13.42 7.60 -0.70 -0.25 6.80 6.09 6.54 -1.16 8.89 14.57 14.27 9.86 -1.32
Bud Site -7.74 11.98 6.39 11.57 -4.89 -3.03 11.23 8.36 12.84 -5.67 -6.68 16.42 11.89 16.78 -5.97
Cell Periphery 1.87 1.16 1.69 0.53 0.95 6.42 7.17 12.84 5.33 9.94 5.97 5.36 10.45 3.97 6.32
Cytoplasm -7.68 -36.26 -30.08 -15.99 4.95 -2.41 -13.86 -24.20 -24.39 -8.03 -7.41 -34.94 -39.98 -29.10 -1.75
Cytoplasmic Foci 9.83 47.42 43.48 23.62 -5.99 10.44 36.41 40.74 37.40 8.75 12.70 58.70 60.43 43.95 2.44
Eisosomes 7.53 15.41 15.86 10.99 1.17 9.21 10.67 15.22 11.98 8.62 12.02 18.19 21.02 16.26 7.29
Endoplasmic Reticulum 2.96 2.35 4.75 6.84 7.83 2.25 2.77 5.46 2.63 10.87 3.04 3.35 6.75 3.68 12.23
Endosome 2.42 21.33 21.61 12.32 0.10 -0.65 11.33 13.87 19.95 6.97 1.73 23.57 25.65 23.17 5.11
Golgi 6.15 18.39 12.74 6.02 -3.07 1.37 14.06 13.25 12.14 -1.50 4.48 22.77 18.79 13.40 -3.45
Lipid Particles 4.65 9.89 9.74 2.21 -0.13 5.22 5.04 8.72 4.24 9.58 7.67 10.76 13.56 4.49 7.19
Mitochondria 6.70 11.62 11.09 3.35 -2.02 -3.23 8.43 0.74 3.33 -3.09 4.50 12.39 10.02 4.51 -3.48
Mitotic Spindle -0.72 -2.37 -4.41 -3.50 -2.58 -5.36 -3.38 -8.05 -2.44 -5.73 -5.03 -3.84 -9.05 -3.67 -6.12
None 3.93 0.49 1.50 -1.54 1.52 -0.35 -1.79 -5.48 -0.68 0.84 2.30 -0.46 1.41 -1.34 2.11
Nuclear Periphery 1.15 0.13 0.80 -2.51 5.33 -0.02 -11.05 -5.04 -6.11 8.53 1.18 -1.13 0.40 -6.25 9.65
Nuclear Periphery Foci -4.27 -10.87 -7.46 0.55 8.21 4.88 -6.21 5.18 -0.87 7.38 -0.86 -11.60 -1.72 -0.25 11.29
Nucleolus 5.79 9.91 9.40 2.70 -2.20 -1.12 -1.32 -0.30 1.04 1.13 2.45 8.08 8.21 1.62 -0.48
Nucleus 8.95 -24.76 -7.36 -16.99 20.57 7.67 -17.53 -19.07 -30.72 6.39 12.59 -29.22 -17.30 -34.67 19.99
Peroxisomes -3.25 14.87 13.84 10.68 -3.08 -2.78 8.07 5.21 8.54 -3.43 -3.22 16.87 12.63 13.45 -4.32
Vacuole -1.81 7.77 -9.96 -7.05 -15.73 -11.21 -17.37 -17.73 -5.76 6.54 -8.27 -8.77 -18.05 -8.76 -5.58
Vacuole Periphery 3.63 17.02 12.35 6.35 -2.76 -1.74 6.85 5.99 8.12 -1.16 1.93 17.45 13.92 10.27 -3.06
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Componentsactinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Lysine methyltransferase; involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Efm1

Efm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Efm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available