Standard name | |
---|---|
Human Ortholog | |
Description | Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 6 | 13 | 2 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 3 | 6 | 3 | – | 0 | 2 | 0 | 3 | 3 | 0 | 3 | 1 |
Bud | 3 | 1 | 0 | 7 | 3 | 4 | 9 | 12 | 7 | 12 | 7 | 7 | 2 | 3 | 1 | – | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 6 | 1 | 1 | 3 | 2 | 0 | 0 | 0 | – | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 3 | 5 | 4 | 13 | 4 | 3 | 2 | 3 | 4 | 2 | 3 | 1 | 4 | 4 | 6 | – | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 5 | 3 | 7 | 9 | 9 | 11 | 13 | 13 | 8 | 8 | 7 | 10 | 7 | 14 | – | 6 | 8 | 0 | 0 | 0 | 1 | 1 | 1 |
Endoplasmic Reticulum | 3 | 1 | 0 | 2 | 1 | 3 | 3 | 1 | 3 | 1 | 3 | 4 | 1 | 3 | 3 | – | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 7 | 13 | 12 | 14 | 21 | 30 | 37 | 63 | 15 | 11 | 0 | 18 | 10 | 3 | 23 | – | 6 | 23 | 12 | 19 | 9 | 4 | 15 | 12 |
Golgi | 2 | 7 | 2 | 36 | 32 | 4 | 3 | 3 | 0 | 2 | 0 | 5 | 20 | 20 | 33 | – | 6 | 6 | 6 | 13 | 1 | 1 | 19 | 8 |
Mitochondria | 188 | 156 | 66 | 174 | 128 | 235 | 277 | 334 | 245 | 297 | 204 | 311 | 74 | 59 | 58 | – | 1 | 8 | 162 | 142 | 101 | 33 | 137 | 137 |
Nucleus | 0 | 0 | 0 | 1 | 3 | 7 | 10 | 5 | 2 | 9 | 6 | 7 | 3 | 1 | 2 | – | 1 | 4 | 0 | 1 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 10 | 3 | 0 | 2 | 1 | 16 | 20 | 10 | 13 | 8 | 9 | 18 | 0 | 1 | 0 | – | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 7 | 3 | 0 | 2 | 2 | 6 | 10 | 18 | 8 | 11 | 8 | 6 | 2 | 1 | 0 | – | 2 | 1 | 0 | 1 | 1 | 0 | 1 | 1 |
Peroxisomes | 7 | 9 | 7 | 7 | 13 | 11 | 10 | 23 | 5 | 16 | 1 | 3 | 5 | 2 | 20 | – | 5 | 20 | 14 | 9 | 12 | 3 | 14 | 6 |
SpindlePole | 6 | 6 | 10 | 14 | 13 | 16 | 25 | 38 | 12 | 13 | 6 | 15 | 4 | 5 | 15 | – | 6 | 16 | 0 | 4 | 1 | 1 | 2 | 3 |
Vac/Vac Membrane | 16 | 48 | 22 | 5 | 21 | 32 | 24 | 26 | 19 | 28 | 13 | 18 | 11 | 7 | 13 | – | 6 | 19 | 2 | 19 | 6 | 1 | 10 | 6 |
Unique Cell Count | 199 | 192 | 97 | 222 | 192 | 306 | 361 | 440 | 284 | 343 | 228 | 360 | 113 | 88 | 134 | 36 | 91 | 206 | 222 | 141 | 54 | 215 | 190 | |
Labelled Cell Count | 257 | 259 | 133 | 297 | 253 | 377 | 444 | 559 | 348 | 420 | 271 | 423 | 149 | 122 | 191 | 41 | 118 | 206 | 222 | 141 | 54 | 215 | 190 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.7 | 6.3 | 6.5 | 7.1 | 5.9 | 5.7 | 6.0 | 5.2 | 5.3 | 5.0 | 5.1 | 6.4 | 6.7 | 6.7 | – | 8.1 | 7.6 | 5.8 | 6.8 | 7.0 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.4 | 1.2 | 1.4 | 1.4 | 1.1 | 1.4 | 1.1 | 1.2 | 1.4 | 0.9 | 0.9 | 1.0 | 1.3 | – | 2.0 | 1.9 | 1.2 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.16 | -0.11 | -0.16 | -0.07 | -0.3 | -0.27 | -0.35 | -0.31 | 0.01 | 0.07 | 0.09 | – | 0.35 | 0.25 | -0.12 | 0.11 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 2.1 | – | 0 | 1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endosome | -1.8 | -0.4 | -0.7 | -0.6 | 0.5 | -2.3 | -3.6 | 0 | -2.6 | -0.8 | -2.2 | 1.0 | – | 0 | 2.3 |
Golgi | 3.6 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 4.3 | 4.7 | – | 0 | 0 |
Mitochondria | 2.0 | -0.2 | 1.7 | 1.8 | 1.6 | 4.0 | 4.2 | 4.7 | 4.2 | -0.4 | -0.1 | -3.7 | – | -6.7 | -8.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | -0.1 | 0 | 0 | -0.8 | 0 | -1.0 | 0 | 0 | -0.9 | 0 | 1.8 | – | 0 | 2.9 |
SpindlePole | -1.2 | -1.0 | -1.8 | -1.1 | -0.5 | -2.2 | -2.5 | 0 | -2.4 | -2.0 | -1.2 | 0.2 | – | 0 | 1.4 |
Vacuole | -6.0 | -2.6 | -3.1 | -4.7 | -5.2 | -4.4 | -4.0 | -4.5 | -5.5 | -2.6 | -2.8 | -2.7 | – | -0.8 | -0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.6973 | 1.7376 | 1.4497 | 1.5807 | 0.8629 | 1.4444 | 1.6706 | 2.2876 | 2.0498 | 1.8676 | 1.2956 | 1.8662 | 1.2866 | 1.6396 | 1.5121 | 1.2897 | 1.4355 | 1.4794 |
Actin | 0.0331 | 0.0285 | 0.0133 | 0.0058 | 0.0318 | 0.0041 | 0.0127 | 0.0091 | 0.0077 | 0.0228 | 0.0053 | 0.0184 | 0.0418 | 0.0204 | 0.0125 | 0.0016 | 0.0754 | 0.0056 |
Bud | 0.0007 | 0.0017 | 0.0068 | 0.0003 | 0.0004 | 0.0003 | 0.0005 | 0.0005 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0007 | 0.0057 | 0.0018 | 0.005 | 0.0012 | 0.0002 |
Bud Neck | 0.0009 | 0.0006 | 0.0004 | 0.0002 | 0.0058 | 0.0005 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0011 | 0.0007 | 0.0011 | 0.0099 | 0.0003 | 0.0046 | 0.0003 |
Bud Periphery | 0.0012 | 0.0013 | 0.0045 | 0.0008 | 0.0008 | 0.0005 | 0.001 | 0.0007 | 0.001 | 0.0008 | 0.0005 | 0.0008 | 0.0011 | 0.0094 | 0.0022 | 0.0034 | 0.0017 | 0.0004 |
Bud Site | 0.0042 | 0.0145 | 0.0103 | 0.0004 | 0.0017 | 0.0006 | 0.0026 | 0.0085 | 0.001 | 0.0008 | 0.0006 | 0.0005 | 0.0019 | 0.0184 | 0.0081 | 0.0044 | 0.0032 | 0.0003 |
Cell Periphery | 0.0004 | 0.0004 | 0.0013 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0007 | 0.0004 | 0.0068 | 0.0004 | 0.0003 |
Cytoplasm | 0.0022 | 0.0119 | 0.0045 | 0.0002 | 0.0007 | 0.0003 | 0.0016 | 0.0004 | 0.0014 | 0.0008 | 0.0004 | 0.0002 | 0.002 | 0.001 | 0.0054 | 0.002 | 0.0042 | 0.0005 |
Cytoplasmic Foci | 0.0487 | 0.0304 | 0.0129 | 0.007 | 0.0276 | 0.0104 | 0.0253 | 0.0204 | 0.0192 | 0.0361 | 0.0118 | 0.0088 | 0.0288 | 0.0235 | 0.0244 | 0.0273 | 0.0123 | 0.0277 |
Eisosomes | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0005 | 0.0004 | 0.0003 | 0.0007 | 0.0005 |
Endoplasmic Reticulum | 0.0023 | 0.0005 | 0.0011 | 0.0001 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0038 | 0.0002 | 0.002 | 0.0003 | 0.0028 | 0.0002 |
Endosome | 0.0444 | 0.0647 | 0.0372 | 0.0253 | 0.0365 | 0.0363 | 0.053 | 0.0214 | 0.032 | 0.0805 | 0.0245 | 0.0247 | 0.0677 | 0.0342 | 0.0289 | 0.029 | 0.0609 | 0.0261 |
Golgi | 0.0545 | 0.0706 | 0.0328 | 0.0369 | 0.0817 | 0.0307 | 0.0472 | 0.0342 | 0.0352 | 0.054 | 0.0236 | 0.0457 | 0.0482 | 0.0507 | 0.0206 | 0.0238 | 0.0743 | 0.0366 |
Lipid Particles | 0.0499 | 0.036 | 0.0109 | 0.0093 | 0.0175 | 0.0238 | 0.05 | 0.0187 | 0.0167 | 0.039 | 0.024 | 0.0252 | 0.058 | 0.039 | 0.0123 | 0.0155 | 0.0229 | 0.0061 |
Mitochondria | 0.7052 | 0.6885 | 0.805 | 0.8959 | 0.6685 | 0.8612 | 0.7102 | 0.8268 | 0.82 | 0.7323 | 0.8469 | 0.8045 | 0.675 | 0.7352 | 0.7996 | 0.8246 | 0.6681 | 0.881 |
None | 0.0014 | 0.0019 | 0.0062 | 0.0001 | 0.0006 | 0.0001 | 0.0061 | 0.0001 | 0.0006 | 0.0005 | 0.0004 | 0.0001 | 0.0018 | 0.0004 | 0.0071 | 0.0074 | 0.0008 | 0.0006 |
Nuclear Periphery | 0.0027 | 0.0003 | 0.0034 | 0.0001 | 0 | 0.0001 | 0.0052 | 0.0001 | 0.0004 | 0.0004 | 0.0009 | 0.0003 | 0.0022 | 0.0001 | 0.0012 | 0.0002 | 0.0043 | 0.0003 |
Nucleolus | 0.0006 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0004 | 0.0012 | 0.0002 | 0.0005 | 0.0006 | 0.0018 | 0.0014 | 0.0012 | 0.0002 |
Nucleus | 0.0006 | 0.0003 | 0.0043 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0005 | 0.0003 | 0.0008 | 0.0005 | 0.0024 | 0.0001 |
Peroxisomes | 0.0366 | 0.031 | 0.0296 | 0.0142 | 0.1232 | 0.0228 | 0.0697 | 0.0555 | 0.0593 | 0.0243 | 0.0404 | 0.0634 | 0.0497 | 0.0453 | 0.0519 | 0.0394 | 0.0499 | 0.0095 |
Punctate Nuclear | 0.0035 | 0.0004 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0038 | 0.0001 | 0.0002 | 0.0015 | 0.0082 | 0.0002 | 0.0023 | 0.0001 | 0.0008 | 0.0011 | 0.0033 | 0.0002 |
Vacuole | 0.003 | 0.009 | 0.0102 | 0.0013 | 0.0005 | 0.0037 | 0.0044 | 0.0009 | 0.002 | 0.0026 | 0.0067 | 0.002 | 0.0046 | 0.0044 | 0.0044 | 0.003 | 0.0027 | 0.001 |
Vacuole Periphery | 0.0033 | 0.0067 | 0.0043 | 0.0014 | 0.0008 | 0.0035 | 0.0028 | 0.0013 | 0.0017 | 0.0017 | 0.0031 | 0.0027 | 0.0081 | 0.0089 | 0.0035 | 0.0027 | 0.003 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.1203 | 3.6475 | 8.1197 | 9.7927 | 6.0459 | 5.8256 | 10.9321 | 8.0007 | 7.2389 | 10.2574 |
Translational Efficiency | 0.6404 | 0.938 | 0.7019 | 0.5917 | 0.8155 | 0.8233 | 0.4132 | 0.6331 | 0.7897 | 0.5359 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
174 | 434 | 148 | 523 | 2084 | 1555 | 265 | 185 | 2258 | 1989 | 413 | 708 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 653.20 | 819.09 | 960.30 | 935.62 | 816.24 | 774.91 | 1041.13 | 1048.56 | 803.68 | 784.55 | 1012.16 | 965.13 |
Standard Deviation | 80.72 | 147.67 | 130.37 | 134.96 | 105.88 | 111.94 | 117.44 | 130.22 | 112.86 | 122.02 | 128.23 | 142.65 |
Intensity Change Log 2 | — | 0.326497 | 0.555960 | 0.518398 | — | -0.074965 | 0.351085 | 0.361344 | — | 0.117385 | 0.445765 | 0.433276 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000581 | 0.006493 | 0.009483 | 0.029235 | 0.000981 | 0.001128 | 0.009025 | 0.013427 | 0.000950 | 0.002299 | 0.009189 | 0.025105 |
Bud Neck | 0.013959 | 0.004622 | 0.024864 | 0.004345 | 0.000889 | 0.004509 | 0.007759 | 0.010675 | 0.001897 | 0.004534 | 0.013888 | 0.005999 |
Bud Site | 0.012937 | 0.014844 | 0.045089 | 0.048470 | 0.003089 | 0.004895 | 0.028190 | 0.076064 | 0.003848 | 0.007066 | 0.034246 | 0.055680 |
Cell Periphery | 0.003043 | 0.000460 | 0.000328 | 0.001000 | 0.000168 | 0.000222 | 0.000279 | 0.000569 | 0.000390 | 0.000274 | 0.000297 | 0.000887 |
Cytoplasm | 0.004464 | 0.002491 | 0.004565 | 0.009151 | 0.000681 | 0.002764 | 0.005390 | 0.008106 | 0.000972 | 0.002704 | 0.005094 | 0.008878 |
Cytoplasmic Foci | 0.025112 | 0.045770 | 0.115438 | 0.043560 | 0.028859 | 0.037757 | 0.119406 | 0.080201 | 0.028570 | 0.039505 | 0.117984 | 0.053134 |
Eisosomes | 0.000095 | 0.000118 | 0.000173 | 0.000181 | 0.000048 | 0.000057 | 0.000119 | 0.000138 | 0.000052 | 0.000071 | 0.000138 | 0.000170 |
Endoplasmic Reticulum | 0.000690 | 0.000493 | 0.005370 | 0.001159 | 0.000193 | 0.000288 | 0.001349 | 0.001472 | 0.000231 | 0.000333 | 0.002790 | 0.001241 |
Endosome | 0.041803 | 0.105199 | 0.127858 | 0.080703 | 0.065851 | 0.101770 | 0.170735 | 0.100329 | 0.063998 | 0.102518 | 0.155370 | 0.085831 |
Golgi | 0.030415 | 0.066867 | 0.080836 | 0.069545 | 0.061843 | 0.075311 | 0.107718 | 0.141592* | 0.059422 | 0.073469 | 0.098085 | 0.088371 |
Lipid Particles | 0.021017 | 0.004791 | 0.013965 | 0.002286 | 0.004764 | 0.007125 | 0.006341 | 0.001176 | 0.006017 | 0.006616 | 0.009073 | 0.001996 |
Mitochondria | 0.775181* | 0.606553* | 0.180231* | 0.561647* | 0.779590* | 0.681947* | 0.266417* | 0.403388* | 0.779250* | 0.665496* | 0.235532* | 0.520294* |
Mitotic Spindle | 0.001994 | 0.019627 | 0.179128* | 0.039999 | 0.006605 | 0.010061 | 0.111352* | 0.039098 | 0.006250 | 0.012149 | 0.135640* | 0.039764 |
None | 0.000499 | 0.000319 | 0.000256 | 0.000254 | 0.000115 | 0.000416 | 0.000165 | 0.000203 | 0.000145 | 0.000395 | 0.000198 | 0.000240 |
Nuclear Periphery | 0.000106 | 0.000210 | 0.003125 | 0.000360 | 0.000323 | 0.000223 | 0.000605 | 0.000104 | 0.000306 | 0.000220 | 0.001508 | 0.000293 |
Nuclear Periphery Foci | 0.000169 | 0.000447 | 0.002722 | 0.000549 | 0.000436 | 0.000442 | 0.001657 | 0.000252 | 0.000416 | 0.000443 | 0.002039 | 0.000471 |
Nucleolus | 0.000578 | 0.001190 | 0.004810 | 0.000074 | 0.000217 | 0.001650 | 0.002122 | 0.000085 | 0.000245 | 0.001549 | 0.003085 | 0.000077 |
Nucleus | 0.006771 | 0.000550 | 0.011414 | 0.000157 | 0.000172 | 0.001271 | 0.004134 | 0.000239 | 0.000681 | 0.001114 | 0.006743 | 0.000178 |
Peroxisomes | 0.037881 | 0.102339* | 0.118715* | 0.074862 | 0.032016 | 0.040573 | 0.091681 | 0.080273 | 0.032468 | 0.054050 | 0.101369* | 0.076276 |
Vacuole | 0.009483 | 0.009181 | 0.057715 | 0.018259 | 0.005087 | 0.014156 | 0.043255 | 0.029762 | 0.005425 | 0.013071 | 0.048437 | 0.021265 |
Vacuole Periphery | 0.013221 | 0.007434 | 0.013914 | 0.014206 | 0.008073 | 0.013435 | 0.022302 | 0.012847 | 0.008470 | 0.012126 | 0.019296 | 0.013851 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.46 | -3.18 | -6.45 | -4.79 | -3.76 | -0.31 | -3.52 | -3.89 | -3.82 | -1.12 | -2.50 | -4.64 | -7.11 | -6.65 | -4.16 |
Bud Neck | 2.26 | -1.82 | 2.36 | 0.22 | 4.49 | -4.58 | -4.51 | -3.24 | -1.98 | -0.85 | -3.61 | -6.13 | -4.07 | -1.27 | 3.68 |
Bud Site | -0.36 | -3.69 | -5.04 | -5.41 | -0.32 | -2.14 | -6.06 | -6.35 | -6.19 | -3.95 | -3.12 | -7.92 | -10.22 | -9.53 | -3.38 |
Cell Periphery | 1.48 | 1.55 | 1.16 | -2.64 | -3.35 | -1.87 | -3.46 | -3.04 | -2.61 | -2.16 | 0.84 | 0.67 | -2.47 | -4.08 | -3.92 |
Cytoplasm | 0.80 | -0.14 | -1.73 | -3.87 | -2.50 | -3.18 | -3.28 | -4.87 | -3.32 | -1.45 | -3.09 | -4.23 | -6.26 | -4.57 | -2.41 |
Cytoplasmic Foci | -2.93 | -6.88 | -2.61 | 0.50 | 5.86 | -3.87 | -9.98 | -6.10 | -4.97 | 3.22 | -5.09 | -12.36 | -6.45 | -3.39 | 8.17 |
Eisosomes | -1.25 | -3.59 | -3.61 | -2.90 | -0.21 | -2.23 | -6.43 | -6.89 | -6.29 | -1.08 | -4.12 | -9.02 | -8.15 | -6.86 | -1.78 |
Endoplasmic Reticulum | 0.67 | -3.66 | -1.08 | -1.77 | 3.27 | -1.51 | -4.09 | -2.30 | -2.12 | -0.16 | -1.61 | -5.08 | -3.39 | -3.02 | 2.80 |
Endosome | -6.40 | -6.86 | -4.34 | 2.54 | 3.78 | -7.18 | -9.15 | -3.65 | 0.17 | 4.94 | -8.58 | -10.64 | -3.77 | 2.68 | 7.21 |
Golgi | -4.67 | -4.37 | -4.99 | -0.38 | 0.94 | -3.16 | -5.35 | -6.07 | -4.97 | -2.24 | -3.72 | -5.65 | -5.03 | -2.49 | 1.13 |
Lipid Particles | 2.09 | 0.72 | 2.48 | 1.47 | 2.01 | -1.78 | -0.84 | 3.47 | 4.34 | 2.68 | -0.51 | -1.23 | 3.85 | 4.16 | 2.91 |
Mitochondria | 5.88 | 18.71 | 7.61 | 1.82 | -13.63 | 9.02 | 24.67 | 13.12 | 9.54 | -3.96 | 11.34 | 33.16 | 16.29 | 8.76 | -13.54 |
Mitotic Spindle | -4.82 | -7.74 | -8.56 | -3.56 | 5.97 | -2.49 | -8.81 | -6.04 | -5.32 | 5.55 | -4.19 | -11.44 | -9.24 | -7.35 | 8.10 |
None | 1.05 | 1.85 | 1.92 | 0.72 | 0.07 | -3.63 | -2.32 | -4.57 | 2.57 | -1.39 | -3.65 | -2.38 | -4.34 | 2.27 | -1.60 |
Nuclear Periphery | -1.14 | -1.32 | -2.54 | -1.19 | 1.21 | 0.51 | -1.15 | 1.16 | 1.97 | 3.10 | 0.48 | -1.43 | 0.06 | -0.87 | 1.47 |
Nuclear Periphery Foci | -2.00 | -3.48 | -2.71 | -0.50 | 2.93 | -0.04 | -2.40 | 0.77 | 2.19 | 3.05 | -0.14 | -3.66 | -0.25 | -0.22 | 3.87 |
Nucleolus | -1.01 | -2.98 | 2.41 | 1.63 | 3.37 | -3.13 | -1.34 | 1.65 | 3.43 | 1.43 | -3.37 | -2.72 | 2.32 | 3.82 | 2.89 |
Nucleus | 1.41 | -1.01 | 1.51 | 1.35 | 4.33 | -3.08 | -1.51 | -0.84 | 2.89 | 1.48 | -1.08 | -3.10 | 1.43 | 3.26 | 3.39 |
Peroxisomes | -5.78 | -4.85 | -3.80 | 2.37 | 2.58 | -2.33 | -5.44 | -3.67 | -2.99 | 0.68 | -5.74 | -7.57 | -6.52 | -3.14 | 2.32 |
Vacuole | 0.08 | -5.15 | -2.14 | -3.16 | 4.36 | -7.93 | -6.65 | -4.01 | -2.57 | 1.56 | -7.58 | -8.81 | -6.38 | -3.24 | 5.05 |
Vacuole Periphery | 1.60 | -0.14 | -0.25 | -2.41 | -0.14 | -4.93 | -4.17 | -1.77 | 0.23 | 2.22 | -3.69 | -4.53 | -2.75 | -0.85 | 1.80 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|