Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 11 | 3 | 6 | 0 | 6 | 3 | 9 | 8 | 3 | 5 | 6 | 0 | 1 | 3 | 0 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 38 | 85 | 70 | 84 | 114 | 135 | 157 | 186 | 108 | 162 | 54 | 121 | 116 | 235 | 348 | 96 | 108 | 174 | 56 | 90 | 77 | 56 | 196 | 210 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 9 | 6 | 14 | 4 | 9 | 0 | 0 | 0 | 2 | 5 | 7 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 1 |
Unique Cell Count | 38 | 88 | 71 | 84 | 114 | 139 | 164 | 201 | 118 | 180 | 61 | 139 | 116 | 235 | 348 | 96 | 108 | 175 | 61 | 94 | 80 | 59 | 208 | 222 |
Labelled Cell Count | 42 | 97 | 73 | 90 | 114 | 143 | 167 | 206 | 122 | 184 | 64 | 139 | 116 | 236 | 351 | 98 | 114 | 188 | 61 | 94 | 80 | 59 | 208 | 222 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 432.5 | 440.5 | 412.9 | 408.0 | 336.7 | 228.7 | 175.4 | 155.0 | 133.3 | 129.2 | 120.8 | 119.7 | 369.8 | 356.9 | 339.9 | 250.1 | 345.2 | 415.0 | 359.8 | 422.0 | 414.8 |
Std Deviation (1e-4) | 73.0 | 68.8 | 75.0 | 87.9 | 71.5 | 64.0 | 51.5 | 42.1 | 35.8 | 34.0 | 31.2 | 35.2 | 107.2 | 75.7 | 74.2 | 69.1 | 70.9 | 98.8 | 107.6 | 109.6 | 149.0 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.29 | -0.85 | -1.24 | -1.41 | -1.63 | -1.68 | -1.77 | -1.79 | -0.16 | -0.21 | -0.28 | -0.72 | -0.26 | 0.01 | -0.2 | 0.03 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.1 | 1.3 | -0.7 | -1.1 | -1.9 | -2.0 | -2.3 | -2.4 | -2.8 | 1.3 | 1.8 | 2.2 | 1.2 | 1.2 | 0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.002 | 0.0019 | 0.0005 | 0.0004 | 0.0414 | 0.0068 | 0.0038 | 0.0012 | 0.0015 | 0.0007 | 0.1283 | 0.0008 | 0.0298 | 0.0011 | 0.0065 | 0.0535 | 0.0005 | 0.016 |
Bud | 0.0022 | 0.0024 | 0.0024 | 0.0013 | 0.0039 | 0.0074 | 0.0053 | 0.0047 | 0.0059 | 0.0066 | 0.0065 | 0.0041 | 0.0056 | 0.005 | 0.0077 | 0.0034 | 0.0045 | 0.0084 |
Bud Neck | 0.0014 | 0.0011 | 0.0013 | 0.0012 | 0.0312 | 0.0064 | 0.0009 | 0.0008 | 0.0012 | 0.0011 | 0.0018 | 0.0031 | 0.0099 | 0.0005 | 0.0016 | 0.0033 | 0.0018 | 0.002 |
Bud Periphery | 0.0041 | 0.001 | 0.0019 | 0.0007 | 0.0066 | 0.0079 | 0.0052 | 0.0031 | 0.0019 | 0.0019 | 0.0053 | 0.0021 | 0.0048 | 0.0012 | 0.0026 | 0.0014 | 0.0008 | 0.0041 |
Bud Site | 0.0012 | 0.002 | 0.0008 | 0.0004 | 0.0305 | 0.0011 | 0.0033 | 0.0017 | 0.0019 | 0.0007 | 0.0027 | 0.0004 | 0.0149 | 0.0016 | 0.0069 | 0.0031 | 0.0002 | 0.001 |
Cell Periphery | 0.0914 | 0.0188 | 0.0647 | 0.047 | 0.0456 | 0.0564 | 0.0264 | 0.0123 | 0.0132 | 0.0105 | 0.0021 | 0.0044 | 0.0351 | 0.0066 | 0.0116 | 0.0023 | 0.0008 | 0.0028 |
Cytoplasm | 0.4172 | 0.8265 | 0.6422 | 0.5177 | 0.2331 | 0.4356 | 0.6456 | 0.8925 | 0.8105 | 0.6936 | 0.5721 | 0.7887 | 0.5356 | 0.8836 | 0.7708 | 0.4325 | 0.8867 | 0.351 |
Cytoplasmic Foci | 0.0059 | 0.0018 | 0.0073 | 0.0007 | 0.007 | 0.0016 | 0.0013 | 0.0004 | 0.0043 | 0.0012 | 0.0064 | 0.0007 | 0.0118 | 0.0005 | 0.0022 | 0.0622 | 0.0004 | 0.0011 |
Eisosomes | 0.0009 | 0.0006 | 0.0002 | 0 | 0.0051 | 0.0009 | 0.0009 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0019 | 0.0001 | 0.0004 | 0.0053 | 0 | 0.0007 |
Endoplasmic Reticulum | 0.0341 | 0.0032 | 0.0071 | 0.0025 | 0.0094 | 0.0178 | 0.0108 | 0.001 | 0.0035 | 0.0006 | 0.0085 | 0.0023 | 0.0061 | 0.0005 | 0.001 | 0.0087 | 0.0008 | 0.0052 |
Endosome | 0.0087 | 0.0027 | 0.002 | 0.0021 | 0.006 | 0.0061 | 0.0066 | 0.0004 | 0.0006 | 0.0013 | 0.0407 | 0.0016 | 0.0026 | 0.0002 | 0.0019 | 0.0434 | 0.0006 | 0.0026 |
Golgi | 0.0012 | 0.0009 | 0.0002 | 0.0001 | 0.003 | 0.0012 | 0.0025 | 0.0001 | 0.0001 | 0.0001 | 0.0339 | 0.0001 | 0.003 | 0.0001 | 0.0014 | 0.0211 | 0 | 0.0013 |
Lipid Particles | 0.0078 | 0.0018 | 0.0021 | 0.0013 | 0.0056 | 0.003 | 0.001 | 0.0001 | 0.0006 | 0.0003 | 0.0084 | 0.0001 | 0.003 | 0.0001 | 0.0029 | 0.0537 | 0 | 0.0003 |
Mitochondria | 0.0263 | 0.0099 | 0.0087 | 0.0017 | 0.0484 | 0.0411 | 0.0393 | 0.0035 | 0.0226 | 0.0038 | 0.0123 | 0.003 | 0.0846 | 0.0185 | 0.051 | 0.039 | 0.0016 | 0.3674 |
None | 0.009 | 0.0016 | 0.002 | 0.0006 | 0.0114 | 0.003 | 0.0033 | 0.0006 | 0.0054 | 0.0015 | 0.0012 | 0.0007 | 0.0111 | 0.0013 | 0.0035 | 0.0623 | 0.0003 | 0.0047 |
Nuclear Periphery | 0.0876 | 0.0162 | 0.024 | 0.0208 | 0.0503 | 0.0694 | 0.0422 | 0.0073 | 0.0158 | 0.0166 | 0.011 | 0.0256 | 0.0513 | 0.0077 | 0.0195 | 0.0332 | 0.0094 | 0.1137 |
Nucleolus | 0.0276 | 0.0046 | 0.0164 | 0.0177 | 0.1764 | 0.0053 | 0.0061 | 0.0007 | 0.0259 | 0.0053 | 0.0006 | 0.0016 | 0.0175 | 0.0025 | 0.0131 | 0.0567 | 0.0002 | 0.003 |
Nucleus | 0.0304 | 0.0168 | 0.0196 | 0.025 | 0.066 | 0.0382 | 0.0615 | 0.0177 | 0.0398 | 0.0684 | 0.015 | 0.0476 | 0.0984 | 0.051 | 0.0693 | 0.0515 | 0.0417 | 0.0631 |
Peroxisomes | 0.0015 | 0.0005 | 0.0003 | 0.0001 | 0.0036 | 0.0006 | 0.0006 | 0.0002 | 0.0007 | 0.0003 | 0.0012 | 0.0002 | 0.0077 | 0.0004 | 0.003 | 0.0248 | 0.0001 | 0.0018 |
Punctate Nuclear | 0.0024 | 0.0002 | 0.0003 | 0.0001 | 0.0021 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0014 | 0.0001 | 0.0006 | 0.0189 | 0 | 0.0004 |
Vacuole | 0.1692 | 0.0636 | 0.1558 | 0.2874 | 0.1135 | 0.1645 | 0.0835 | 0.0408 | 0.0324 | 0.1208 | 0.1041 | 0.069 | 0.0473 | 0.0107 | 0.0137 | 0.0095 | 0.0362 | 0.008 |
Vacuole Periphery | 0.0678 | 0.0217 | 0.0402 | 0.0713 | 0.1 | 0.1254 | 0.0498 | 0.0108 | 0.012 | 0.0646 | 0.0365 | 0.0438 | 0.0166 | 0.0068 | 0.0089 | 0.01 | 0.0133 | 0.0415 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1237.9311 | 1422.6333 | 1856.8901 | 1425.7014 | 1639.359 | 1504.7197 | 1323.9443 | 1264.1856 | 1468.1287 | 1226.3579 |
Translational Efficiency | 1.4235 | 1.4813 | 1.3362 | 1.6374 | 1.4616 | 1.1897 | 1.5118 | 1.6909 | 1.4182 | 1.6646 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
150 | 212 | 2305 | 1523 | 1459 | 1854 | 2361 | 1401 | 1609 | 2066 | 4666 | 2924 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 16361.91 | 25653.59 | 35900.86 | 26302.05 | 21764.27 | 26347.59 | 33135.41 | 29479.48 | 21260.63 | 26276.38 | 34501.54 | 27824.48 |
Standard Deviation | 4565.01 | 6050.62 | 6318.89 | 7999.01 | 4344.73 | 5466.73 | 6823.14 | 7924.95 | 4639.70 | 5533.49 | 6722.59 | 8120.27 |
Intensity Change Log 2 | — | 0.648820 | 1.133677 | 0.684834 | — | 0.275709 | 0.606412 | 0.437749 | — | 0.447762 | 0.856573 | 0.549006 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000237 | 0.000112 | 0.000065 | 0.000198 | 0.000037 | 0.000047 | 0.000070 | 0.000161 | 0.000056 | 0.000054 | 0.000068 | 0.000180 |
Bud Neck | 0.000778 | 0.001206 | 0.000286 | 0.002298 | 0.000267 | 0.000263 | 0.000421 | 0.001203 | 0.000314 | 0.000359 | 0.000355 | 0.001773 |
Bud Site | 0.006021 | 0.009554 | 0.000875 | 0.004162 | 0.001013 | 0.000984 | 0.001375 | 0.006429 | 0.001480 | 0.001864 | 0.001128 | 0.005248 |
Cell Periphery | 0.049518 | 0.094767 | 0.095257 | 0.017490 | 0.017739 | 0.012271 | 0.028759 | 0.039907 | 0.020701 | 0.020736 | 0.061609 | 0.028231 |
Cytoplasm | 0.253401* | 0.420478* | 0.398466* | 0.682630* | 0.685176* | 0.790957* | 0.685123* | 0.569625* | 0.644924* | 0.752941* | 0.543515* | 0.628485* |
Cytoplasmic Foci | 0.011909 | 0.013046 | 0.001196 | 0.006696 | 0.008498 | 0.006428 | 0.001781 | 0.003610 | 0.008816 | 0.007107 | 0.001492 | 0.005217 |
Eisosomes | 0.000006 | 0.000005 | 0.000003 | 0.000004 | 0.000002 | 0.000001 | 0.000002 | 0.000003 | 0.000002 | 0.000001 | 0.000002 | 0.000003 |
Endoplasmic Reticulum | 0.001179 | 0.000526 | 0.001985 | 0.000313 | 0.000625 | 0.000401 | 0.000646 | 0.000712 | 0.000677 | 0.000414 | 0.001308 | 0.000504 |
Endosome | 0.001183 | 0.000547 | 0.000157 | 0.000538 | 0.000227 | 0.000207 | 0.000123 | 0.000328 | 0.000316 | 0.000242 | 0.000140 | 0.000438 |
Golgi | 0.140827* | 0.071224 | 0.005559 | 0.003949 | 0.052051 | 0.036623 | 0.005896 | 0.010042 | 0.060327 | 0.040173 | 0.005730 | 0.006869 |
Lipid Particles | 0.035186 | 0.032745 | 0.014165 | 0.007474 | 0.008239 | 0.006130 | 0.008133 | 0.010357 | 0.010751 | 0.008861 | 0.011113 | 0.008855 |
Mitochondria | 0.000801 | 0.000138 | 0.000232 | 0.000818 | 0.000186 | 0.000286 | 0.000242 | 0.000267 | 0.000243 | 0.000271 | 0.000237 | 0.000554 |
Mitotic Spindle | 0.001573 | 0.000991 | 0.000119 | 0.002909 | 0.000421 | 0.000402 | 0.000135 | 0.001092 | 0.000528 | 0.000462 | 0.000127 | 0.002038 |
None | 0.002192 | 0.002265 | 0.000461 | 0.007656 | 0.002696 | 0.004753 | 0.002087 | 0.003981 | 0.002649 | 0.004498 | 0.001284 | 0.005895 |
Nuclear Periphery | 0.003538 | 0.001316 | 0.000860 | 0.000655 | 0.001160 | 0.000675 | 0.000444 | 0.000611 | 0.001381 | 0.000740 | 0.000650 | 0.000634 |
Nuclear Periphery Foci | 0.016153 | 0.011657 | 0.003000 | 0.005677 | 0.012111 | 0.008166 | 0.003760 | 0.005134 | 0.012488 | 0.008524 | 0.003385 | 0.005417 |
Nucleolus | 0.000425 | 0.000123 | 0.000100 | 0.000674 | 0.000070 | 0.000055 | 0.000129 | 0.000244 | 0.000103 | 0.000062 | 0.000115 | 0.000468 |
Nucleus | 0.012653 | 0.017064 | 0.003387 | 0.076396 | 0.011886 | 0.009925 | 0.014428 | 0.027722 | 0.011957 | 0.010657 | 0.008973 | 0.053074 |
Peroxisomes | 0.000041 | 0.000035 | 0.000296 | 0.000075 | 0.000008 | 0.000007 | 0.000068 | 0.000055 | 0.000011 | 0.000010 | 0.000181 | 0.000065 |
Vacuole | 0.215489* | 0.215597* | 0.442172* | 0.168641 | 0.085700 | 0.065069 | 0.225902* | 0.286430* | 0.097799 | 0.080515 | 0.332739* | 0.225078* |
Vacuole Periphery | 0.246891* | 0.106604* | 0.031358 | 0.010748 | 0.111891* | 0.056350 | 0.020476 | 0.032086 | 0.124477* | 0.061507 | 0.025852 | 0.020972 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.25 | 1.69 | 0.34 | -3.50 | -6.05 | -1.36 | -5.44 | -7.57 | -6.35 | -5.08 | 0.15 | -1.11 | -7.37 | -8.36 | -7.84 |
Bud Neck | -2.01 | 1.89 | -10.80 | -9.62 | -11.47 | -0.96 | -6.45 | -6.19 | -5.96 | -5.44 | -1.70 | -2.96 | -13.07 | -12.59 | -12.49 |
Bud Site | -0.77 | 3.33 | 0.02 | 0.82 | -8.86 | 0.07 | -1.80 | -6.79 | -6.86 | -6.34 | -0.76 | 1.26 | -9.04 | -7.03 | -10.22 |
Cell Periphery | -4.11 | -7.31 | 6.65 | 9.02 | 32.59* | 4.15 | -5.87 | -6.92 | -9.83 | -2.63 | 0.10 | -22.11 | -0.98 | -1.02 | 21.31 |
Cytoplasm | -4.58 | -5.41 | -13.46 | -7.52 | -20.07 | -9.26 | 0.49 | 9.76 | 20.10 | 10.52 | -9.27 | 9.93 | 5.16 | 17.42 | -5.96 |
Cytoplasmic Foci | -0.28 | 4.43 | -1.92 | -1.66 | -9.93 | 2.46 | 9.58 | 1.78 | -0.03 | -5.09 | 2.01 | 11.77 | -3.34 | -4.87 | -11.06 |
Eisosomes | 0.41 | 1.79 | 0.79 | 0.52 | -3.09 | 2.33 | -0.46 | -5.95 | -8.18 | -5.03 | 1.74 | -1.54 | -5.93 | -8.08 | -5.25 |
Endoplasmic Reticulum | 2.00 | -2.22 | 2.94 | 2.27 | 9.93 | 1.33 | 0.03 | -0.02 | -2.26 | -0.06 | 1.70 | -3.53 | 1.62 | -0.25 | 8.02 |
Endosome | 3.58 | 6.14 | 2.51 | -0.31 | -2.67 | 0.60 | 5.93 | -4.62 | -4.80 | -7.97 | 2.43 | 7.58 | -2.05 | -2.86 | -4.10 |
Golgi | 5.24 | 11.24 | 11.38 | 11.01 | 4.30 | 6.11 | 23.10 | 20.57 | 16.06 | -4.87 | 7.49 | 24.67 | 24.13 | 21.01 | -2.00 |
Lipid Particles | 0.42 | 4.19 | 5.62 | 5.94 | 7.06 | 2.70 | 0.11 | -1.50 | -3.91 | -1.70 | 1.95 | -0.49 | 2.47 | 0.53 | 3.93 |
Mitochondria | 2.08 | 1.80 | -0.08 | -7.39 | -6.22 | -1.56 | -1.73 | -3.42 | -0.38 | -1.03 | -0.42 | -0.08 | -5.43 | -4.30 | -6.06 |
Mitotic Spindle | 1.92 | 5.33 | -4.62 | -5.14 | -5.83 | -0.04 | 3.71 | -2.68 | -2.68 | -3.08 | 0.45 | 5.23 | -5.98 | -6.05 | -6.57 |
None | 0.04 | 4.12 | -4.26 | -4.51 | -6.51 | -2.43 | 1.57 | -1.21 | 0.81 | -1.79 | -2.42 | 5.79 | -3.94 | -0.73 | -5.86 |
Nuclear Periphery | 0.92 | 1.12 | 0.84 | -1.11 | -5.24 | 5.62 | 9.06 | 4.73 | -0.60 | -3.57 | 2.66 | 3.08 | 1.00 | -4.56 | -6.40 |
Nuclear Periphery Foci | 2.00 | 6.69 | 4.34 | 3.01 | -5.24 | 4.21 | 9.30 | 6.93 | 3.88 | -2.37 | 4.54 | 11.61 | 7.05 | 3.55 | -5.54 |
Nucleolus | 1.17 | 1.26 | -4.26 | -9.62 | -9.78 | 1.45 | -3.00 | -4.38 | -4.44 | -3.85 | 1.47 | -0.60 | -9.97 | -10.53 | -10.01 |
Nucleus | -0.63 | 1.46 | -14.38 | -18.78 | -24.83 | 1.07 | -3.40 | -9.92 | -10.72 | -8.08 | 0.62 | 1.14 | -22.95 | -24.01 | -24.56 |
Peroxisomes | 0.38 | -8.35 | -2.89 | -4.00 | 6.38 | 0.11 | -7.44 | -6.74 | -6.75 | 0.33 | 0.22 | -12.13 | -8.21 | -8.33 | 5.94 |
Vacuole | -0.20 | -11.55 | 3.08 | 3.70 | 34.21* | 3.85 | -20.68 | -22.32 | -25.73 | -5.41 | 2.97 | -38.87 | -18.41 | -22.44 | 17.99 |
Vacuole Periphery | 6.58 | 11.10 | 12.33 | 10.26 | 18.85* | 10.72 | 20.13 | 17.54 | 9.22 | -4.66 | 12.11 | 21.46 | 23.02 | 16.29 | 7.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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