Standard name
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0.07
Bud 0 0.05 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.05 0.06 0.06 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0 0.09 0 0.06 0.09 0.08
Mitochondria 0.78 0.79 0.82 0.83 0.9 0.9 0.91 0.87 0.89 0.93 0.91 0.95 0.85 0.75 0.68 0.1 0.09 0.71 0.66 0.65 0.63 0.55 0.49
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0 0.06 0 0.06 0.16 0.17 0.21 0.11 0.21 0.11 0.16 0 0 0 0.1 0.12 0 0 0 0 0 0
Peroxisomes 0.16 0.16 0.15 0.23 0.06 0 0 0 0 0 0 0 0.16 0.23 0.34 0.42 0.28 0.07 0 0.09 0.14 0.13 0.16
SpindlePole 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.19 0 0 0 0 0.06 0.07
Vac/Vac Membrane 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 0 1 0 1 1 2 2 3 1 1 5 6 7 6 12 29
Bud 7 6 4 5 4 3 0 6 3 0 3 3 6 15 13 2 3 3 1 1 3 4 10
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 3
Bud Site 4 0 1 2 0 0 1 0 0 0 2 0 1 1 0 4 1
Cell Periphery 7 2 1 0 0 2 1 0 1 0 1 0 6 1 6 5 2 0 0 0 0 0 0
Cytoplasm 2 1 2 1 1 8 7 7 10 6 6 8 7 6 9 4 3 1 0 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 2 3 0 0 0 1 0 0 0 3 0 1 3 0 0 1 0 0 0
Endosome 11 3 1 0 0 0 0 0 0 1 1 0 10 16 10 4 8 13 8 6 5 15 22
Golgi 0 4 0 0 0 0 1 1 0 0 1 0 3 6 8 1 18 7 12 3 14 33 31
Mitochondria 211 102 65 59 86 157 186 165 143 210 139 240 334 353 377 6 7 191 89 67 144 200 199
Nucleus 1 1 0 0 0 0 1 2 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0
Nuclear Periphery 11 0 2 0 2 2 7 8 7 4 5 3 2 2 2 2 1 1 0 0 1 3 2
Nucleolus 33 5 5 3 6 27 34 39 18 47 17 40 11 15 14 6 9 0 1 1 1 6 2
Peroxisomes 44 20 12 16 6 1 2 0 0 0 2 1 63 110 188 26 22 19 5 9 31 45 66
SpindlePole 20 4 3 3 2 0 2 1 0 0 0 0 8 21 20 5 15 10 2 1 9 20 28
Vac/Vac Membrane 13 13 2 1 2 6 5 7 2 6 1 4 21 17 22 5 8 1 5 1 6 8 6
Unique Cell Count 269 129 79 71 96 174 204 190 161 225 153 252 395 469 552 62 78 270 136 104 231 362 410
Labelled Cell Count 364 162 99 90 111 209 248 236 185 275 179 300 474 568 672 74 101 270 136 104 231 362 410


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 12.9 10.0 10.4 22.0 26.4 27.1 28.3 25.4 28.1 24.4 26.0 16.6 17.4 18.4 15.6 20.1 11.0 11.2 12.3
Std Deviation (1e-4) 2.6 4.4 3.5 3.1 8.1 10.3 11.1 10.9 9.9 12.6 12.6 10.6 5.9 6.2 5.8 7.1 10.3 3.8 3.6 3.8
Intensity Change (Log2) 0.06 1.14 1.4 1.44 1.5 1.35 1.49 1.29 1.38 0.73 0.8 0.88 0.65 1.01 0.13 0.17 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 4.5
Mitochondria 0.1 1.4 1.8 2.1 1.0 1.4 2.9 1.9 3.7 0.5 -1.4 -2.5 -8.6 -9.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.0 2.3 2.9 1.2 3.0 1.2 2.2 0 0 0 0 1.1
Peroxisomes 1.2 -1.9 0 0 0 0 0 0 0 0.2 1.6 3.4 3.6 2.0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available