Standard name | |
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Human Ortholog | |
Description | Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 35 | 2 | 53 | 65 | 74 | 48 | 63 | 55 | 29 | 33 | 21 | 25 | 23 | 47 | 35 | 19 | 1 | 11 | 28 | 7 | 27 | 16 | 46 | 51 |
Bud | 14 | 0 | 34 | 51 | 66 | 42 | 43 | 48 | 41 | 46 | 33 | 35 | 15 | 24 | 29 | 19 | 4 | 9 | 26 | 9 | 37 | 50 | 76 | 93 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 12 | 34 | 15 | 28 | 19 |
Bud Site | 2 | 0 | 5 | 6 | 3 | 9 | 6 | 7 | 4 | 8 | 4 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 24 | 1 | 36 | 47 | 45 | 36 | 64 | 54 | 49 | 62 | 52 | 67 | 46 | 71 | 90 | 68 | 35 | 60 | 19 | 2 | 12 | 1 | 0 | 4 |
Cytoplasm | 84 | 17 | 143 | 238 | 372 | 382 | 499 | 452 | 251 | 316 | 237 | 300 | 71 | 115 | 152 | 153 | 71 | 132 | 75 | 24 | 138 | 21 | 44 | 84 |
Endoplasmic Reticulum | 20 | 2 | 15 | 23 | 41 | 31 | 56 | 39 | 15 | 16 | 28 | 26 | 17 | 14 | 5 | 12 | 2 | 6 | 5 | 0 | 12 | 1 | 4 | 12 |
Endosome | 0 | 0 | 1 | 3 | 6 | 0 | 5 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 |
Golgi | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 1 | 1 | 23 | 20 | 89 | 103 | 119 | 137 | 152 | 132 | 167 | 1 | 0 | 5 | 7 | 0 | 3 | 1 | 0 | 6 | 0 | 1 | 2 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 7 | 1 | 4 | 2 | 1 | 0 | 0 | 1 | 11 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 3 | 1 | 0 | 5 | 7 | 2 | 15 | 45 | 9 | 10 | 15 | 21 | 0 | 0 | 1 | 5 | 2 | 9 | 0 | 0 | 5 | 0 | 1 | 3 |
Unique Cell Count | 156 | 21 | 228 | 356 | 524 | 526 | 703 | 697 | 414 | 492 | 407 | 493 | 134 | 198 | 228 | 223 | 95 | 181 | 176 | 61 | 279 | 114 | 213 | 282 |
Labelled Cell Count | 187 | 24 | 289 | 464 | 638 | 640 | 857 | 832 | 538 | 651 | 526 | 648 | 174 | 273 | 318 | 301 | 117 | 234 | 176 | 61 | 279 | 114 | 213 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.5 | 9.5 | 8.0 | 7.8 | 7.6 | 6.6 | 6.3 | 6.3 | 5.5 | 5.5 | 5.5 | 5.3 | 11.8 | 11.5 | 9.7 | 12.3 | 14.0 | 14.7 | 10.8 | 11.5 | 12.6 |
Std Deviation (1e-4) | 1.9 | 1.1 | 1.1 | 1.1 | 1.0 | 1.2 | 1.2 | 1.0 | 0.9 | 0.8 | 1.0 | 1.0 | 1.8 | 2.0 | 2.0 | 2.4 | 2.8 | 2.7 | 2.1 | 2.1 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.07 | -0.27 | -0.35 | -0.34 | -0.54 | -0.54 | -0.54 | -0.59 | 0.57 | 0.53 | 0.29 | 0.63 | 0.81 | 0.88 | 0.44 | 0.54 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.5 | -3.1 | -5.2 | -5.7 | -6.3 | -5.9 | -6.4 | -6.8 | -7.3 | -1.4 | 0.1 | -2.1 | -4.3 | -4.9 | -4.7 |
Bud | -0.2 | -0.9 | -2.9 | -4.2 | -3.7 | -1.9 | -2.2 | -2.7 | -3.3 | -1.0 | -0.8 | -0.7 | -2.1 | -2.7 | -3.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.9 | -2.9 | -3.8 | -2.8 | -3.6 | -1.4 | -1.2 | -1.1 | -0.8 | 4.1 | 4.8 | 5.7 | 3.7 | 4.2 | 4.1 |
Cytoplasm | 1.0 | 2.2 | 2.7 | 2.3 | 0.6 | -0.5 | 0.4 | -1.1 | -0.5 | -1.8 | -1.0 | 0.9 | 1.3 | 2.1 | 2.2 |
Endoplasmic Reticulum | -0.1 | 0.6 | -0.4 | 0.7 | -0.5 | -1.7 | -2.0 | 0.1 | -0.7 | 2.0 | 0.2 | -2.3 | -0.5 | -1.6 | -1.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.6 | 2.6 | 6.4 | 5.9 | 6.5 | 9.6 | 9.3 | 9.5 | 9.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.9 | 0 | 0 | 2.9 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.9757 | 8.571 | 8.0018 | 7.7366 | 7.039 | 8.4161 | 8.2468 | 10.788 | 9.9278 | 10.1286 | 9.1261 | 10.3438 | 7.0997 | 7.9451 | 7.6627 | 7.3758 | 7.0965 | 7.6918 |
Actin | 0.0325 | 0.0065 | 0.0275 | 0.0327 | 0.0517 | 0.0205 | 0.0273 | 0.0123 | 0.0228 | 0.0104 | 0.0442 | 0.0106 | 0.0373 | 0.0256 | 0.0289 | 0.017 | 0.0326 | 0.0367 |
Bud | 0.0324 | 0.0381 | 0.199 | 0.1275 | 0.063 | 0.0094 | 0.0389 | 0.0398 | 0.239 | 0.1784 | 0.1238 | 0.0126 | 0.0454 | 0.0285 | 0.2215 | 0.1331 | 0.1098 | 0.0163 |
Bud Neck | 0.0217 | 0.0071 | 0.0093 | 0.003 | 0.0284 | 0.2541 | 0.0295 | 0.0045 | 0.0054 | 0.0336 | 0.0052 | 0.3009 | 0.0166 | 0.0054 | 0.0049 | 0.0174 | 0.0354 | 0.2093 |
Bud Periphery | 0.1049 | 0.0607 | 0.4297 | 0.4843 | 0.3073 | 0.0441 | 0.0988 | 0.044 | 0.3443 | 0.492 | 0.4043 | 0.0419 | 0.1114 | 0.0464 | 0.4044 | 0.4619 | 0.4465 | 0.0505 |
Bud Site | 0.1497 | 0.2413 | 0.0424 | 0.006 | 0.0091 | 0.0101 | 0.1316 | 0.2046 | 0.0339 | 0.0066 | 0.0232 | 0.0125 | 0.1271 | 0.306 | 0.0483 | 0.0133 | 0.0152 | 0.0266 |
Cell Periphery | 0.0958 | 0.0313 | 0.0186 | 0.0346 | 0.0672 | 0.0352 | 0.0569 | 0.0162 | 0.0113 | 0.0233 | 0.031 | 0.0229 | 0.1166 | 0.0145 | 0.0146 | 0.0286 | 0.0442 | 0.0309 |
Cytoplasm | 0.4399 | 0.5762 | 0.2448 | 0.1892 | 0.2978 | 0.5812 | 0.4601 | 0.6524 | 0.2936 | 0.2335 | 0.2966 | 0.5491 | 0.4821 | 0.5511 | 0.2633 | 0.2898 | 0.3075 | 0.5934 |
Cytoplasmic Foci | 0.0141 | 0.0032 | 0.0018 | 0.0021 | 0.0056 | 0.0073 | 0.0132 | 0.007 | 0.0053 | 0.0013 | 0.0031 | 0.0099 | 0.0082 | 0.0028 | 0.0014 | 0.0008 | 0.0009 | 0.0072 |
Eisosomes | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0011 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0.0014 |
Endoplasmic Reticulum | 0.0093 | 0.0048 | 0.0023 | 0.0058 | 0.0124 | 0.0078 | 0.0214 | 0.0017 | 0.0017 | 0.0017 | 0.0046 | 0.0063 | 0.0144 | 0.0029 | 0.0019 | 0.0014 | 0.0014 | 0.0057 |
Endosome | 0.0168 | 0.0025 | 0.006 | 0.0083 | 0.0576 | 0.0074 | 0.0302 | 0.0028 | 0.0174 | 0.0007 | 0.0289 | 0.0091 | 0.0075 | 0.0015 | 0.0016 | 0.0026 | 0.0005 | 0.0035 |
Golgi | 0.0072 | 0.0015 | 0.0034 | 0.0079 | 0.0095 | 0.0034 | 0.0119 | 0.001 | 0.007 | 0.0002 | 0.005 | 0.0054 | 0.0038 | 0.001 | 0.0008 | 0.0004 | 0.0005 | 0.0047 |
Lipid Particles | 0.0096 | 0.0006 | 0.0005 | 0.0035 | 0.0025 | 0.0006 | 0.0189 | 0.0002 | 0.001 | 0.0001 | 0.0005 | 0.0019 | 0.0028 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0011 |
Mitochondria | 0.0176 | 0.0004 | 0.0011 | 0.048 | 0.0442 | 0.0062 | 0.012 | 0.0002 | 0.0042 | 0.0002 | 0.0039 | 0.0029 | 0.0033 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0.0008 |
None | 0.0078 | 0.0007 | 0.0005 | 0.0005 | 0.0073 | 0.0006 | 0.0057 | 0.0007 | 0.0007 | 0.0071 | 0.0091 | 0.0007 | 0.0027 | 0.0013 | 0.0007 | 0.0003 | 0.0004 | 0.0012 |
Nuclear Periphery | 0.0039 | 0.0008 | 0.001 | 0.0011 | 0.0027 | 0.0004 | 0.0084 | 0.0005 | 0.0007 | 0.0009 | 0.0013 | 0.001 | 0.0023 | 0.0006 | 0.0005 | 0.0006 | 0.0003 | 0.0003 |
Nucleolus | 0.0013 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0014 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.004 | 0.0022 | 0.0014 | 0.0011 | 0.0019 | 0.001 | 0.0053 | 0.0022 | 0.0014 | 0.0016 | 0.0016 | 0.0014 | 0.0014 | 0.0016 | 0.0009 | 0.0017 | 0.0005 | 0.0009 |
Peroxisomes | 0.004 | 0.0001 | 0.0004 | 0.0012 | 0.0007 | 0.0004 | 0.0056 | 0.0002 | 0.0008 | 0 | 0.0002 | 0.0015 | 0.0021 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0009 |
Punctate Nuclear | 0.0035 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0043 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0009 | 0.0025 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 |
Vacuole | 0.0217 | 0.02 | 0.0089 | 0.0399 | 0.0268 | 0.0089 | 0.0159 | 0.0088 | 0.008 | 0.0076 | 0.0115 | 0.0069 | 0.0115 | 0.0093 | 0.0053 | 0.0277 | 0.0032 | 0.0074 |
Vacuole Periphery | 0.0019 | 0.0011 | 0.001 | 0.0026 | 0.0027 | 0.0008 | 0.0021 | 0.0005 | 0.0011 | 0.0007 | 0.0017 | 0.0008 | 0.0006 | 0.0005 | 0.0004 | 0.0027 | 0.0002 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.3677 | 27.9823 | 32.4439 | 33.9753 | 24.6045 | 21.1173 | 34.924 | 31.508 | 30.7757 | 35.6967 |
Translational Efficiency | 1.2335 | 1.2037 | 1.0063 | 0.9634 | 1.2666 | 1.7081 | 0.947 | 1.0387 | 1.0193 | 0.8584 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
329 | 1243 | 989 | 470 | 1440 | 1935 | 859 | 1391 | 1769 | 3178 | 1848 | 1861 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1026.30 | 1129.93 | 1504.84 | 1345.41 | 933.39 | 1101.61 | 1407.57 | 1355.07 | 950.67 | 1112.69 | 1459.63 | 1352.63 |
Standard Deviation | 127.74 | 190.50 | 230.05 | 198.29 | 157.21 | 159.37 | 171.86 | 190.63 | 156.40 | 172.77 | 210.73 | 192.64 |
Intensity Change Log 2 | — | 0.138781 | 0.552158 | 0.390593 | — | 0.239062 | 0.592655 | 0.537815 | — | 0.187414 | 0.571588 | 0.462590 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004834 | 0.003166 | 0.017629 | 0.008800 | 0.002471 | 0.003797 | 0.008470 | 0.007515 | 0.002910 | 0.003550 | 0.013372 | 0.007839 |
Bud Neck | 0.024375 | 0.016384 | 0.018855 | 0.007612 | 0.014919 | 0.023708 | 0.010795 | 0.005611 | 0.016678 | 0.020843 | 0.015108 | 0.006117 |
Bud Site | 0.520554* | 0.442354* | 0.451627* | 0.389129* | 0.349487* | 0.518916* | 0.572933* | 0.287337* | 0.381302* | 0.488971* | 0.508014* | 0.313045* |
Cell Periphery | 0.021139 | 0.003776 | 0.026235 | 0.005989 | 0.005119 | 0.003784 | 0.003489 | 0.004407 | 0.008098 | 0.003781 | 0.015662 | 0.004807 |
Cytoplasm | 0.386132* | 0.471961* | 0.431473* | 0.532912* | 0.539037* | 0.411480* | 0.343296* | 0.659729* | 0.510600* | 0.435136* | 0.390486* | 0.627701* |
Cytoplasmic Foci | 0.026885 | 0.036456 | 0.012181 | 0.012777 | 0.051833 | 0.019735 | 0.021122 | 0.007457 | 0.047193 | 0.026275 | 0.016337 | 0.008800 |
Eisosomes | 0.000342 | 0.000042 | 0.000178 | 0.000064 | 0.000081 | 0.000069 | 0.000053 | 0.000049 | 0.000130 | 0.000059 | 0.000120 | 0.000053 |
Endoplasmic Reticulum | 0.001355 | 0.001301 | 0.001287 | 0.000181 | 0.002704 | 0.000548 | 0.000710 | 0.000208 | 0.002453 | 0.000842 | 0.001019 | 0.000201 |
Endosome | 0.001368 | 0.004156 | 0.001474 | 0.001616 | 0.006022 | 0.002113 | 0.001557 | 0.000774 | 0.005157 | 0.002912 | 0.001513 | 0.000986 |
Golgi | 0.002905 | 0.003750 | 0.002544 | 0.001203 | 0.005386 | 0.004032 | 0.000406 | 0.001025 | 0.004925 | 0.003922 | 0.001550 | 0.001070 |
Lipid Particles | 0.000537 | 0.000620 | 0.008844 | 0.002642 | 0.000929 | 0.000454 | 0.001527 | 0.002397 | 0.000857 | 0.000519 | 0.005443 | 0.002459 |
Mitochondria | 0.000334 | 0.000197 | 0.000123 | 0.003206 | 0.002056 | 0.001540 | 0.000206 | 0.000387 | 0.001736 | 0.001015 | 0.000161 | 0.001099 |
Mitotic Spindle | 0.000102 | 0.000473 | 0.000623 | 0.000308 | 0.000514 | 0.000668 | 0.000292 | 0.001239 | 0.000437 | 0.000591 | 0.000469 | 0.001004 |
None | 0.000163 | 0.000182 | 0.000308 | 0.000040 | 0.000554 | 0.000297 | 0.000060 | 0.000071 | 0.000481 | 0.000252 | 0.000193 | 0.000064 |
Nuclear Periphery | 0.000005 | 0.000012 | 0.000030 | 0.000041 | 0.000019 | 0.000019 | 0.000015 | 0.000010 | 0.000016 | 0.000016 | 0.000023 | 0.000018 |
Nuclear Periphery Foci | 0.000174 | 0.000559 | 0.002453 | 0.000804 | 0.000450 | 0.000412 | 0.001311 | 0.000546 | 0.000398 | 0.000469 | 0.001922 | 0.000611 |
Nucleolus | 0.000021 | 0.000074 | 0.000029 | 0.000052 | 0.000430 | 0.000047 | 0.000021 | 0.000017 | 0.000354 | 0.000058 | 0.000025 | 0.000025 |
Nucleus | 0.001444 | 0.001206 | 0.005244 | 0.001498 | 0.002297 | 0.000970 | 0.002414 | 0.001800 | 0.002139 | 0.001062 | 0.003928 | 0.001724 |
Peroxisomes | 0.000743 | 0.001193 | 0.000744 | 0.001371 | 0.001135 | 0.001162 | 0.001124 | 0.001162 | 0.001062 | 0.001174 | 0.000921 | 0.001214 |
Vacuole | 0.006032 | 0.010687 | 0.017570 | 0.027267 | 0.011511 | 0.005043 | 0.029730 | 0.017496 | 0.010492 | 0.007250 | 0.023222 | 0.019964 |
Vacuole Periphery | 0.000556 | 0.001451 | 0.000550 | 0.002489 | 0.003045 | 0.001206 | 0.000467 | 0.000763 | 0.002582 | 0.001302 | 0.000511 | 0.001199 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.59 | -9.44 | -3.02 | -4.62 | 4.99 | -2.85 | -8.92 | -6.49 | -4.37 | 0.68 | -1.89 | -13.89 | -7.40 | -6.24 | 5.49 |
Bud Neck | 2.19 | 1.61 | 4.70 | 5.28 | 6.54 | -4.90 | 3.43 | 8.16 | 11.73 | 5.16 | -2.82 | 1.48 | 9.20 | 13.18 | 9.64 |
Bud Site | 3.53 | 3.41 | 6.40 | 4.45 | 4.47 | -14.38 | -12.51 | 5.83 | 21.86* | 17.83* | -10.37 | -9.16 | 7.43 | 20.18 | 17.45* |
Cell Periphery | 4.62 | -1.06 | 4.07 | -1.86 | 9.20 | 1.75 | 1.93 | 1.00 | -1.11 | -1.38 | 4.56 | -4.95 | 3.55 | -1.95 | 8.96 |
Cytoplasm | -4.03 | -2.35 | -6.57 | -4.02 | -5.90 | 10.98 | 12.85 | -10.56 | -23.12 | -22.76 | 7.44 | 9.82 | -11.42 | -21.33 | -22.40 |
Cytoplasmic Foci | -2.15 | 3.53 | 2.44 | 6.19 | -1.28 | 10.66 | 7.79 | 15.44 | 7.39 | 7.39 | 7.92 | 11.07 | 15.00 | 10.67 | 6.11 |
Eisosomes | 4.41 | 2.39 | 4.04 | -2.96 | 6.23 | 1.13 | 3.94 | 4.47 | 2.16 | 0.70 | 4.80 | 0.71 | 5.38 | 0.83 | 6.91 |
Endoplasmic Reticulum | 0.07 | 0.17 | 2.77 | 2.46 | 5.41 | 3.90 | 3.22 | 4.66 | 2.11 | 1.54 | 3.32 | 3.03 | 5.09 | 3.13 | 4.29 |
Endosome | -3.90 | -0.58 | -0.46 | 3.16 | -0.15 | 4.92 | 5.53 | 6.94 | 3.62 | 3.73 | 3.28 | 5.72 | 6.68 | 5.12 | 3.05 |
Golgi | -1.42 | 0.76 | 2.96 | 3.95 | 2.52 | 1.68 | 10.14 | 7.80 | 4.34 | -2.08 | 1.69 | 7.73 | 8.28 | 5.87 | 1.76 |
Lipid Particles | -0.54 | -9.29 | -4.33 | -4.08 | 5.34 | 2.23 | -3.20 | -4.17 | -5.06 | -1.94 | 2.05 | -9.33 | -5.50 | -6.30 | 4.49 |
Mitochondria | 1.44 | 2.13 | -1.43 | -1.51 | -1.55 | 0.63 | 2.76 | 2.42 | 2.21 | -0.66 | 1.18 | 2.91 | 1.03 | 0.03 | -1.74 |
Mitotic Spindle | -3.72 | -3.14 | -2.00 | 0.79 | 1.50 | -0.43 | 0.51 | -0.95 | -0.74 | -1.23 | -0.59 | -0.42 | -1.00 | -0.74 | -0.87 |
None | -0.44 | -1.26 | 3.57 | 3.65 | 2.25 | 1.09 | 3.37 | 3.32 | 1.25 | -0.24 | 1.41 | 2.17 | 3.50 | 1.65 | 2.06 |
Nuclear Periphery | -1.44 | -14.34 | -1.87 | -1.43 | -0.51 | -0.01 | -0.72 | 1.46 | 0.60 | 3.79 | -0.01 | -2.61 | -0.39 | -0.25 | 1.61 |
Nuclear Periphery Foci | -1.69 | -7.22 | -5.10 | -1.55 | 4.11 | 0.16 | -4.31 | -1.25 | -0.85 | 3.94 | -0.45 | -7.59 | -2.99 | -1.35 | 6.41 |
Nucleolus | -1.21 | -1.45 | -0.87 | 0.53 | -0.57 | 1.36 | 1.45 | 1.47 | 1.68 | 1.47 | 1.28 | 1.43 | 1.44 | 1.50 | 0.19 |
Nucleus | 1.05 | -9.36 | -2.19 | -3.01 | 6.34 | 3.25 | -2.06 | 0.65 | -5.74 | 4.19 | 3.26 | -6.06 | 0.10 | -6.63 | 8.67 |
Peroxisomes | -2.55 | 0.12 | -2.80 | -0.66 | -3.01 | -0.09 | -0.10 | -0.22 | -0.07 | -0.16 | -0.51 | 1.17 | -0.84 | -0.22 | -1.62 |
Vacuole | -3.43 | -9.16 | -7.60 | -6.46 | -4.68 | 6.70 | -8.49 | -5.56 | -11.37 | 5.83 | 3.55 | -10.13 | -8.92 | -11.37 | 1.68 |
Vacuole Periphery | -3.16 | 0.00 | -1.48 | -0.66 | -1.48 | 3.74 | 5.44 | 3.86 | 0.95 | -0.59 | 3.25 | 5.71 | 2.69 | 0.42 | -1.38 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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