Standard name
Human Ortholog
Description Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0.13 0.06 0.05 0.1 0.08 0.12 0.11
Bud 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 – 0.12 0.23 0 0 0 0.05 0.08 0.09
Cytoplasm 0.53 0.56 0.62 0.46 0.55 0.48 – 0.44 0.43 0.43 0.4 0.36 0.77 0.77 0.89 – 0.74 0.55 0.06 0.18 0 0.12 0.1 0.13
Endoplasmic Reticulum 0.4 0.39 0.08 0.14 0 0.09 – 0 0.05 0.05 0.08 0.07 0.18 0.19 0.06 – 0 0 0.7 0.53 0.67 0.43 0.41 0.37
Endosome 0 0 0.12 0.11 0.16 0 – 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.08 0.13 0.33 0.18 0.5 – 0.56 0.55 0.61 0.67 0.62 0 0 0 – 0.07 0.08 0.09 0.14 0.12 0.22 0.2 0.19
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0.06 0.15 0.07 – 0.06 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 8 0 1 0 0 – 0 0 3 2 4 4 5 4 – 5 13 13 22 29 16 44 37
Bud 0 4 1 5 2 4 – 15 17 15 21 12 1 1 0 – 1 0 1 5 1 1 1 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 2 1 2 – 7 1 3 8 2 2 7 7
Bud Site 0 1 0 0 0 0 – 1 2 3 1 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 3 8 6 11 5 6 – 11 10 10 13 9 7 7 1 – 22 23 2 8 6 9 31 30
Cytoplasm 90 199 136 105 125 132 – 257 173 195 164 140 130 130 133 – 131 56 15 78 8 24 37 46
Endoplasmic Reticulum 69 139 17 31 9 24 – 20 21 21 33 28 30 32 9 – 7 3 163 234 197 88 157 128
Endosome 2 0 26 24 36 6 – 27 11 13 2 9 3 1 1 – 5 2 5 2 2 4 7 6
Golgi 1 0 0 2 0 0 – 0 0 0 0 0 0 0 0 – 0 0 2 2 1 0 2 3
Mitochondria 9 30 28 76 41 137 – 331 220 275 279 239 0 0 0 – 13 8 21 60 36 45 77 68
Nucleus 0 0 0 0 2 1 – 0 1 2 3 2 2 2 1 – 3 2 0 0 0 0 1 0
Nuclear Periphery 0 1 1 1 0 1 – 1 1 1 1 2 0 0 0 – 0 0 0 3 1 2 1 0
Nucleolus 0 0 0 0 0 0 – 0 1 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 7 3 8 0 – 12 1 5 0 12 0 0 0 – 6 1 0 0 1 0 1 0
SpindlePole 1 1 1 1 1 1 – 8 0 3 1 3 1 2 0 – 6 5 0 2 0 0 0 1
Vac/Vac Membrane 3 5 14 14 34 20 – 34 23 12 8 15 1 1 2 – 5 1 4 8 2 5 8 10
Unique Cell Count 171 356 219 228 227 275 589 403 452 414 385 168 169 150 177 101 234 443 297 206 385 352
Labelled Cell Count 188 396 237 274 263 332 717 481 559 529 475 181 182 153 211 115 234 443 297 206 385 352


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.8 5.2 5.7 5.2 4.6 – 4.3 4.1 3.9 3.9 4.1 6.0 6.0 6.2 4.7 5.4 6.6 6.5 6.5 6.9
Std Deviation (1e-4) 0.8 0.6 1.8 2.3 1.4 2.2 – 1.1 1.2 0.9 1.1 0.9 0.8 0.8 1.4 0.8 0.9 1.6 1.2 1.3 1.9
Intensity Change (Log2) – – – 0.14 0.01 -0.18 – -0.28 -0.33 -0.43 -0.41 -0.35 0.21 0.21 0.26 -0.16 0.05 0.34 0.31 0.31 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – -1.7 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5891 3.2965 2.9085 3.1661 1.9719 2.4123 5.0946 4.7781 5.1835 4.8769 4.48 4.7019 5.4816 6.0501 5.8648 5.7186 5.3936 5.5197
Actin 0.0182 0.0249 0.0258 0.0148 0.1449 0.0078 0.0985 0.0047 0.005 0.0016 0.0484 0.0026 0.0101 0.005 0.0075 0.002 0.0018 0.0035
Bud 0.0003 0.0001 0.0004 0.0016 0.0035 0.0002 0.0009 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0003 0 0 0.0013
Bud Neck 0.0193 0.0025 0.0013 0.0056 0.0041 0.0144 0.0046 0.0004 0.0016 0.0055 0.0016 0.0011 0.0003 0.0004 0.0029 0.0011 0.0009 0.0039
Bud Periphery 0.0004 0.0001 0.0005 0.0028 0.0035 0.0004 0.0016 0.0006 0.0003 0.0016 0.0004 0.0002 0.0003 0.001 0.0005 0.0002 0.0001 0.0017
Bud Site 0.01 0.0019 0.0022 0.0034 0.0577 0.0002 0.0063 0.0011 0.0037 0.0003 0.0013 0.0001 0.0011 0.0032 0.0034 0.0002 0.0002 0.0003
Cell Periphery 0.0031 0.004 0.002 0.0028 0.0024 0.0024 0.0045 0.0136 0.0083 0.0084 0.0031 0.002 0.0084 0.0045 0.0074 0.0064 0.0027 0.0039
Cytoplasm 0.0791 0.1051 0.0987 0.2 0.0391 0.1139 0.061 0.1276 0.1349 0.1096 0.1239 0.0901 0.0578 0.1144 0.098 0.0294 0.0459 0.135
Cytoplasmic Foci 0.0828 0.0158 0.0175 0.027 0.0109 0.0064 0.0242 0.0045 0.014 0.0219 0.0153 0.0069 0.0072 0.0095 0.0079 0.0094 0.0067 0.01
Eisosomes 0.0003 0.0003 0.0002 0.0001 0.0011 0.0003 0.0009 0.0006 0.0003 0.0004 0.0005 0.0005 0.0005 0.0003 0.0004 0.0002 0.0002 0.0003
Endoplasmic Reticulum 0.5751 0.7442 0.7322 0.6162 0.6476 0.7576 0.6094 0.7629 0.7485 0.7668 0.6759 0.8214 0.8458 0.7707 0.7759 0.8894 0.865 0.7612
Endosome 0.0567 0.0167 0.03 0.0172 0.017 0.0234 0.0254 0.0067 0.0155 0.0083 0.0279 0.0131 0.0113 0.0187 0.0206 0.0155 0.0208 0.0192
Golgi 0.0132 0.0084 0.0139 0.0113 0.0097 0.0094 0.0135 0.0045 0.0043 0.0027 0.0146 0.0019 0.0055 0.0054 0.0094 0.0069 0.006 0.0057
Lipid Particles 0.0597 0.0518 0.037 0.008 0.0122 0.0117 0.0246 0.015 0.0234 0.0163 0.0591 0.0233 0.0238 0.0362 0.0255 0.0252 0.0344 0.0172
Mitochondria 0.0012 0.0014 0.0038 0.0505 0.0074 0.0018 0.0063 0.0003 0.001 0.0014 0.0024 0.0004 0.0004 0.0031 0.0052 0.0011 0.0019 0.0055
None 0.0146 0.0022 0.0026 0.0208 0.0028 0.0349 0.0345 0.044 0.0159 0.0412 0.0135 0.0267 0.0134 0.0074 0.0168 0.002 0.0015 0.0209
Nuclear Periphery 0.0102 0.0096 0.0107 0.0051 0.0105 0.0081 0.0262 0.0063 0.0086 0.0056 0.0047 0.0048 0.0066 0.0097 0.0092 0.0055 0.0055 0.0048
Nucleolus 0.0005 0.0001 0.0002 0.0003 0.0009 0.0001 0.0023 0.0002 0.0002 0.0002 0.0001 0 0 0.0001 0.0001 0 0.0001 0.0001
Nucleus 0.0016 0.0006 0.0009 0.0015 0.003 0.0009 0.0164 0.001 0.0012 0.0007 0.0008 0.0005 0.0004 0.0034 0.0009 0.0004 0.0006 0.0008
Peroxisomes 0.0258 0.005 0.0131 0.0031 0.0088 0.0004 0.0086 0.0001 0.0048 0.0006 0.0018 0.0001 0.0026 0.0003 0.0004 0.0004 0.0002 0.0003
Punctate Nuclear 0.0222 0.0007 0.0013 0.002 0.0088 0.0005 0.0219 0.0004 0.0013 0.0012 0.0007 0.0003 0.0005 0.0005 0.0005 0.0003 0.0018 0.0004
Vacuole 0.0048 0.004 0.0048 0.004 0.0034 0.0035 0.0055 0.0052 0.0063 0.0053 0.0037 0.0036 0.0037 0.0053 0.0062 0.0037 0.0032 0.0035
Vacuole Periphery 0.0009 0.0005 0.0008 0.0019 0.0007 0.0018 0.0028 0.0003 0.0006 0.0005 0.0004 0.0003 0.0004 0.0006 0.001 0.0005 0.0005 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 236.6512 202.8993 138.6822 151.7402 216.1605 209.434 198.3712 159.2771 151.7965 212.6515
Translational Efficiency 0.7644 0.7889 0.7755 0.7201 0.7145 0.8297 0.6899 0.6605 0.6838 0.6514

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
948 1161 1500 1821 2187 1473 2186 1264 3135 2634 3686 3085

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 887.69 770.52 895.46 1021.04 754.27 797.30 922.69 954.93 794.62 785.50 911.61 993.95
Standard Deviation 116.17 102.03 109.95 149.42 82.98 100.29 149.34 133.84 112.43 101.93 135.37 146.89
Intensity Change Log 2 — -0.204224 0.012573 0.201912 — 0.080042 0.290765 0.340314 — -0.066659 0.147052 0.267142

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001228 0.000757 0.000312 0.004208 0.000188 0.000473 0.001139 0.006611 0.000502 0.000599 0.000802 0.005192
Bud Neck 0.005158 0.007747 0.003863 0.006693 0.003572 0.007224 0.004723 0.011070 0.004051 0.007454 0.004373 0.008487
Bud Site 0.021876 0.003844 0.003579 0.015919 0.001928 0.004350 0.006520 0.034584 0.007960 0.004127 0.005323 0.023567
Cell Periphery 0.000672 0.000474 0.000323 0.002931 0.000612 0.000542 0.000992 0.001531 0.000630 0.000512 0.000720 0.002358
Cytoplasm 0.227938* 0.319609* 0.325206* 0.125419* 0.374353* 0.294540* 0.224008* 0.219074* 0.330078* 0.305590* 0.265190* 0.163792*
Cytoplasmic Foci 0.059831 0.177572 0.025526 0.010416 0.117534 0.145737 0.018562 0.039129 0.100085 0.159769 0.021396 0.022180
Eisosomes 0.000380 0.000652 0.000071 0.000970 0.000940 0.000799 0.000254 0.000769 0.000771 0.000734 0.000179 0.000888
Endoplasmic Reticulum 0.206060* 0.172982* 0.093866 0.693037* 0.197262* 0.220463* 0.481858* 0.367607* 0.199922* 0.199534* 0.323966* 0.559700*
Endosome 0.020051 0.020354 0.009155 0.006601 0.009603 0.017515 0.007083 0.013119 0.012762 0.018766 0.007926 0.009272
Golgi 0.006115 0.011433 0.000191 0.002985 0.014902 0.018754 0.001068 0.003876 0.012245 0.015527 0.000711 0.003350
Lipid Particles 0.052383 0.108473* 0.012310 0.019326 0.126021* 0.146787* 0.016501 0.036420 0.103754* 0.129899* 0.014796 0.026330
Mitochondria 0.003569 0.005631 0.001398 0.002115 0.007276 0.012644 0.001413 0.004445 0.006155 0.009553 0.001407 0.003070
Mitotic Spindle 0.010288 0.000783 0.000770 0.001291 0.000499 0.001940 0.000692 0.003557 0.003459 0.001430 0.000724 0.002220
None 0.002340 0.004280 0.002582 0.001261 0.003705 0.002666 0.003848 0.000674 0.003292 0.003377 0.003333 0.001021
Nuclear Periphery 0.035405 0.006400 0.086005 0.001953 0.006674 0.006556 0.031189 0.006122 0.015362 0.006487 0.053496 0.003662
Nuclear Periphery Foci 0.058102 0.010405 0.065630 0.017043 0.005157 0.007301 0.055362 0.039108 0.021167 0.008669 0.059540 0.026084
Nucleolus 0.000455 0.001113 0.001132 0.000175 0.000622 0.000713 0.000648 0.000285 0.000572 0.000889 0.000845 0.000220
Nucleus 0.069644 0.081970 0.300187* 0.036655 0.098764 0.062194 0.108647 0.082794 0.089959 0.070911 0.186593* 0.055559
Peroxisomes 0.003274 0.005549 0.000594 0.001406 0.004343 0.006734 0.001241 0.002114 0.004020 0.006212 0.000978 0.001696
Vacuole 0.212844* 0.058011 0.066869 0.049160 0.025008 0.039461 0.033974 0.125875 0.081808 0.047637 0.047360 0.080592
Vacuole Periphery 0.002389 0.001961 0.000431 0.000435 0.001037 0.002606 0.000277 0.001234 0.001446 0.002322 0.000340 0.000762

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.28 4.38 -6.11 -6.38 -8.29 -3.18 -6.35 -4.77 -4.55 -3.99 -0.61 -3.12 -7.76 -7.45 -7.15
Bud Neck -2.30 1.22 -2.64 0.16 -3.82 -4.46 -1.93 -7.12 -3.23 -5.30 -5.17 -1.14 -7.76 -2.01 -6.14
Bud Site 5.31 5.29 0.92 -7.29 -7.26 -2.63 -5.26 -9.91 -8.98 -8.22 2.94 1.54 -8.76 -11.47 -10.56
Cell Periphery 1.81 3.90 -7.98 -8.66 -9.21 1.09 -3.02 -4.80 -5.21 -2.26 2.09 -1.48 -9.29 -9.95 -8.17
Cytoplasm -9.06 -9.39 10.43 22.42 23.49 10.21 21.05 18.41 8.15 0.49 3.71 10.86 27.70 22.78 16.86
Cytoplasmic Foci -19.75 10.04 15.46 31.15 9.85 -5.54 31.23 21.77 22.34 -8.61 -15.04 31.98 31.08 39.22 -0.70
Eisosomes -4.08 5.76 -5.64 -2.81 -9.13 1.59 8.23 1.58 0.51 -4.42 0.46 9.95 -1.23 -1.74 -9.23
Endoplasmic Reticulum 0.24 8.71 -38.64 -41.70* -60.03* -2.45 -29.34 -11.41 -8.73 11.33 -1.18 -17.25 -38.32 -35.92 -22.27
Endosome -1.21 6.84 8.89 11.28 4.55 -7.70 3.20 -2.83 3.97 -5.38 -7.40 6.39 4.33 11.71 -1.70
Golgi -3.85 3.46 1.55 9.64 -7.40 -2.99 20.40 12.76 12.28 -3.93 -3.72 17.48 12.37 15.88 -7.25
Lipid Particles -12.52 12.11 8.65 20.62 -5.54 -3.82 32.76* 24.04 23.41 -8.87 -7.05 34.12 27.70 31.92 -9.29
Mitochondria -2.40 1.61 0.97 5.93 -1.42 -3.34 7.55 2.43 4.80 -2.23 -3.47 7.24 4.03 6.97 -2.76
Mitotic Spindle 3.70 3.69 3.18 -1.70 -1.72 -1.77 -1.10 -3.34 -1.59 -3.01 2.06 3.03 0.58 -1.87 -3.36
None -1.77 -0.48 1.06 3.18 3.38 1.39 -0.14 5.87 3.64 4.50 -0.23 -0.12 4.92 4.50 5.06
Nuclear Periphery 8.70 -10.61 10.44 7.51 20.23 0.16 -15.25 1.06 0.82 14.86 7.46 -17.94 10.80 5.49 25.11
Nuclear Periphery Foci 12.62* -2.82 9.95 -5.32 14.47 -2.71 -22.02 -15.33 -13.76 5.61 8.97 -18.93 -4.42 -14.05 15.41
Nucleolus -4.26 -7.08 5.53 6.11 10.15 -0.66 -0.36 3.78 3.62 2.54 -3.00 -2.93 5.64 7.13 6.77
Nucleus -2.40 -32.73 6.82 10.49 39.98 11.13 -2.87 2.75 -5.76 4.81 7.08 -23.96 10.43 3.80 31.19
Peroxisomes -3.79 5.06 3.31 9.86 -3.74 -4.46 7.52 5.57 10.43 -2.55 -5.51 9.44 7.15 14.56 -3.42
Vacuole 21.84 19.93 19.95 -2.70 1.01 -7.00 -6.48 -21.58 -18.56 -19.31 13.27 12.19 -1.62 -15.78 -14.68
Vacuole Periphery 0.10 2.71 2.71 9.12 0.09 -5.81 13.09 -0.23 3.89 -2.92 -4.06 5.45 2.91 7.94 -2.87
Standard name
Human Ortholog
Description Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication
Localization
Cell Percentages ER (89%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Pmt2

Pmt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pmt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available