Standard name | |
---|---|
Human Ortholog | |
Description | Lanosterol 14-alpha-demethylase; catalyzes C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in ergosterol biosynthesis pathway; transcriptionally down-regulated when ergosterol is in excess; member of cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p; human CYP51A1 functionally complements the lethality of the erg11 null mutation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 11 | 19 | 17 | 9 | 17 | 31 | 35 | – | 63 | 34 | 36 | 8 | 8 | 4 | 16 | 15 | 14 | 2 | 5 | 8 | 2 | 7 | 7 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 9 | – | 6 | 2 | 1 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 6 | 1 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 32 | 52 | 21 | 23 | 19 | 15 | 35 | – | 53 | 46 | 51 | 41 | 41 | 67 | 96 | 68 | 69 | 0 | 3 | 15 | 6 | 13 | 17 |
Cytoplasm | 12 | 56 | 22 | 18 | 27 | 28 | 34 | 87 | – | 71 | 41 | 47 | 13 | 13 | 29 | 63 | 63 | 61 | 5 | 22 | 4 | 4 | 3 | 4 |
Endoplasmic Reticulum | 122 | 397 | 385 | 236 | 237 | 344 | 317 | 530 | – | 360 | 252 | 219 | 205 | 205 | 298 | 3 | 9 | 4 | 173 | 449 | 407 | 215 | 333 | 316 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 |
Golgi | 4 | 4 | 60 | 42 | 26 | 4 | 10 | 10 | – | 6 | 1 | 4 | 1 | 1 | 2 | 42 | 72 | 75 | 2 | 0 | 1 | 2 | 5 | 6 |
Mitochondria | 1 | 0 | 0 | 11 | 1 | 18 | 39 | 29 | – | 51 | 51 | 40 | 0 | 0 | 0 | 24 | 17 | 12 | 2 | 2 | 2 | 9 | 9 | 9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | – | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 9 | 3 | 6 | 12 | 14 | 29 | – | 15 | 8 | 18 | 0 | 0 | 4 | 3 | 1 | 2 | 2 | 1 | 1 | 3 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 7 | 7 | 6 | 2 | 17 | 7 | 26 | 26 | – | 24 | 7 | 19 | 0 | 1 | 8 | 13 | 25 | 20 | 2 | 4 | 1 | 6 | 8 | 7 |
Unique Cell Count | 143 | 457 | 436 | 275 | 285 | 389 | 400 | 673 | 511 | 352 | 334 | 235 | 233 | 345 | 230 | 229 | 217 | 198 | 502 | 448 | 256 | 388 | 375 | |
Labelled Cell Count | 151 | 508 | 554 | 350 | 346 | 449 | 486 | 795 | 650 | 442 | 435 | 269 | 270 | 414 | 266 | 275 | 262 | 198 | 502 | 448 | 256 | 388 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 28.9 | 31.6 | 30.4 | 29.8 | 25.4 | 20.2 | 19.6 | 20.1 | – | 18.6 | 18.1 | 18.6 | 24.3 | 24.3 | 20.5 | 20.4 | 25.5 | 28.2 | 30.9 | 28.9 | 29.0 |
Std Deviation (1e-4) | 6.9 | 8.1 | 6.8 | 5.4 | 4.7 | 3.6 | 3.5 | 3.9 | – | 3.9 | 3.7 | 4.3 | 3.9 | 3.9 | 3.5 | 5.5 | 8.1 | 8.5 | 6.7 | 5.5 | 5.6 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.26 | -0.59 | -0.63 | -0.6 | – | -0.71 | -0.75 | -0.71 | -0.33 | -0.33 | -0.57 | -0.58 | -0.26 | -0.11 | 0.02 | -0.08 | -0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.1 | -0.8 | 0 | 2.1 | 0.6 | – | 4.3 | 3.0 | 3.4 | -0.6 | -0.6 | -2.6 | 1.4 | 1.2 | 1.2 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.8 | -1.7 | -3.6 | -4.3 | -4.1 | – | -0.8 | 0.5 | 1.4 | 2.0 | 2.0 | 2.9 | 8.8 | 5.7 | 6.2 |
Cytoplasm | 0.8 | 2.3 | 1.3 | 2.0 | 4.3 | – | 4.6 | 3.4 | 4.3 | 0.3 | 0.3 | 1.9 | 8.2 | 8.2 | 8.3 |
Endoplasmic Reticulum | -1.0 | -2.0 | 0.1 | -3.6 | -4.1 | – | -6.7 | -5.9 | -7.6 | -0.4 | -0.1 | -0.8 | -21.6 | -21.0 | -21.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.6 | -1.9 | -6.8 | -5.9 | -8.2 | – | -7.6 | -7.0 | -6.3 | -5.7 | -5.7 | -6.8 | 1.5 | 5.4 | 6.2 |
Mitochondria | 0 | 0 | 4.5 | 6.7 | 4.4 | – | 6.8 | 8.2 | 7.4 | 0 | 0 | 0 | 6.9 | 5.8 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0.9 | 1.3 | 2.0 | – | 0.9 | 0.2 | 2.5 | 0 | 0 | -1.0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.4 | 0.5 | 3.9 | 2.4 | – | 2.9 | 0.7 | 3.3 | 0 | 0 | 1.0 | 3.2 | 5.5 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 30.8618 | 26.9057 | 27.0583 | 27.1531 | 25.0291 | 27.4694 | 32.1926 | 28.5701 | 30.3703 | 29.2481 | 26.2161 | 27.3584 | 34.7827 | 28.9868 | 29.5821 | 28.9988 | 26.058 | 29.8109 |
Actin | 0.0002 | 0.0009 | 0.0014 | 0.0013 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0.0011 | 0.0001 | 0.0005 | 0.0005 | 0.0005 |
Bud | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0002 | 0.001 | 0.0007 | 0.0042 | 0.002 | 0.001 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0013 | 0.0009 |
Bud Periphery | 0.0001 | 0.0004 | 0.0005 | 0.0024 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0 | 0.0018 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0048 | 0.0279 | 0.0207 | 0.0238 | 0.0104 | 0.0043 | 0.0022 | 0.0043 | 0.0024 | 0.0007 | 0.0013 | 0.0026 | 0.008 | 0.0185 | 0.0054 | 0.0048 | 0.0085 | 0.0038 |
Cytoplasm | 0.0276 | 0.0704 | 0.0344 | 0.0773 | 0.0174 | 0.0152 | 0.0018 | 0.0057 | 0.0031 | 0.0002 | 0.0054 | 0.0032 | 0.013 | 0.0378 | 0.0074 | 0.0062 | 0.0035 | 0.0099 |
Cytoplasmic Foci | 0.0001 | 0.001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.0002 | 0 | 0 | 0.0003 |
Eisosomes | 0 | 0.0008 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0.0017 | 0 | 0.0003 | 0.001 | 0 |
Endoplasmic Reticulum | 0.9126 | 0.8121 | 0.9051 | 0.8307 | 0.886 | 0.9468 | 0.9824 | 0.9737 | 0.9879 | 0.9966 | 0.9871 | 0.9891 | 0.895 | 0.9031 | 0.9724 | 0.9555 | 0.9704 | 0.9495 |
Endosome | 0.0006 | 0.0019 | 0.0015 | 0.0013 | 0.0011 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.001 | 0.0004 | 0.0003 | 0.0003 | 0.0008 |
Golgi | 0.0002 | 0.0017 | 0.0029 | 0.0009 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0016 | 0.0015 | 0.0002 | 0.0006 | 0.0012 | 0.0003 |
Lipid Particles | 0.005 | 0.0227 | 0.011 | 0.0031 | 0.0022 | 0.0029 | 0.0011 | 0.0069 | 0.0017 | 0.0001 | 0.0005 | 0.0013 | 0.001 | 0.0079 | 0.0019 | 0.0004 | 0.0006 | 0.0022 |
Mitochondria | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0067 | 0.0027 | 0 | 0.0001 | 0 | 0.0001 |
None | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0009 | 0 | 0.0002 | 0.0001 | 0.0004 |
Nuclear Periphery | 0.0359 | 0.0291 | 0.0102 | 0.035 | 0.0522 | 0.0208 | 0.0102 | 0.0043 | 0.0031 | 0.002 | 0.0027 | 0.0022 | 0.0592 | 0.0133 | 0.009 | 0.0265 | 0.0107 | 0.0257 |
Nucleolus | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0059 | 0.0079 | 0.001 | 0.0038 | 0.0112 | 0.0032 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0041 | 0.0016 | 0.0002 | 0.0023 | 0.0003 | 0.0019 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0006 |
Vacuole | 0.0057 | 0.0182 | 0.0087 | 0.0132 | 0.0159 | 0.004 | 0.0015 | 0.0039 | 0.001 | 0.0003 | 0.0019 | 0.0012 | 0.0066 | 0.0049 | 0.0023 | 0.0013 | 0.0012 | 0.0027 |
Vacuole Periphery | 0.0006 | 0.0007 | 0.0005 | 0.0012 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0013 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 289.8774 | 364.7053 | 366.1966 | 430.9374 | 414.9044 | 224.297 | 347.7799 | 355.7894 | 349.0505 | 432.7325 |
Translational Efficiency | 0.8658 | 1.0356 | 1.0824 | 0.889 | 0.9218 | 0.862 | 0.9679 | 0.8091 | 1.0027 | 0.9181 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
181 | 1355 | 99 | 1090 | 1394 | 1146 | 417 | 1469 | 1575 | 2501 | 516 | 2559 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1690.72 | 2325.80 | 3815.89 | 3360.68 | 1574.52 | 2118.87 | 3620.67 | 3808.09 | 1587.87 | 2230.98 | 3658.12 | 3617.52 |
Standard Deviation | 555.08 | 520.91 | 754.82 | 1075.76 | 336.26 | 463.27 | 584.62 | 829.12 | 369.95 | 505.95 | 625.64 | 967.73 |
Intensity Change Log 2 | — | 0.460089 | 1.174382 | 0.991115 | — | 0.428383 | 1.201345 | 1.274155 | — | 0.444887 | 1.187446 | 1.134537 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005517 | 0.000975 | 0.000518 | 0.003345 | 0.002440 | 0.001377 | 0.000083 | 0.000771 | 0.002794 | 0.001159 | 0.000166 | 0.001867 |
Bud Neck | 0.006787 | 0.000256 | 0.016353 | 0.014213 | 0.000335 | 0.000347 | 0.001886 | 0.003449 | 0.001077 | 0.000298 | 0.004661 | 0.008034 |
Bud Site | 0.043865 | 0.001274 | 0.002738 | 0.044451 | 0.003262 | 0.002926 | 0.000904 | 0.007098 | 0.007928 | 0.002031 | 0.001255 | 0.023009 |
Cell Periphery | 0.036806 | 0.004575 | 0.057843 | 0.028646 | 0.013559 | 0.005710 | 0.022470 | 0.035729 | 0.016231 | 0.005095 | 0.029256 | 0.032712 |
Cytoplasm | 0.028340 | 0.000607 | 0.000196 | 0.027878 | 0.003979 | 0.001098 | 0.000144 | 0.007680 | 0.006779 | 0.000832 | 0.000154 | 0.016284 |
Cytoplasmic Foci | 0.010313 | 0.000434 | 0.000131 | 0.011399 | 0.001730 | 0.001216 | 0.000140 | 0.001995 | 0.002717 | 0.000792 | 0.000138 | 0.006001 |
Eisosomes | 0.000465 | 0.000104 | 0.001685 | 0.000228 | 0.000454 | 0.000098 | 0.001224 | 0.000396 | 0.000456 | 0.000101 | 0.001312 | 0.000325 |
Endoplasmic Reticulum | 0.646774* | 0.972153* | 0.699314* | 0.467385* | 0.936992* | 0.965666* | 0.910312* | 0.813189* | 0.903640* | 0.969181* | 0.869830* | 0.665894* |
Endosome | 0.023808 | 0.003659 | 0.011734 | 0.033828 | 0.004096 | 0.005472 | 0.003518 | 0.010858 | 0.006362 | 0.004490 | 0.005094 | 0.020642 |
Golgi | 0.017160 | 0.003495 | 0.009558 | 0.042991 | 0.006534 | 0.002698 | 0.011050 | 0.016563 | 0.007755 | 0.003130 | 0.010764 | 0.027820 |
Lipid Particles | 0.055756 | 0.003261 | 0.054749 | 0.137790* | 0.002512 | 0.002944 | 0.009880 | 0.038715 | 0.008631 | 0.003116 | 0.018488 | 0.080916 |
Mitochondria | 0.004205 | 0.000602 | 0.000334 | 0.004023 | 0.007015 | 0.001563 | 0.000194 | 0.001197 | 0.006692 | 0.001043 | 0.000221 | 0.002401 |
Mitotic Spindle | 0.009580 | 0.000096 | 0.000221 | 0.014109 | 0.000222 | 0.000482 | 0.000087 | 0.001520 | 0.001297 | 0.000273 | 0.000113 | 0.006882 |
None | 0.000096 | 0.000562 | 0.000019 | 0.005217 | 0.000682 | 0.000254 | 0.000076 | 0.001398 | 0.000614 | 0.000421 | 0.000065 | 0.003025 |
Nuclear Periphery | 0.001955 | 0.000774 | 0.034383 | 0.007070 | 0.001153 | 0.000927 | 0.007906 | 0.005869 | 0.001245 | 0.000844 | 0.012986 | 0.006381 |
Nuclear Periphery Foci | 0.021163 | 0.001471 | 0.022473 | 0.063414 | 0.001512 | 0.002664 | 0.003228 | 0.016105 | 0.003771 | 0.002017 | 0.006921 | 0.036256 |
Nucleolus | 0.000058 | 0.000223 | 0.000025 | 0.000158 | 0.000089 | 0.000049 | 0.000015 | 0.000036 | 0.000085 | 0.000143 | 0.000017 | 0.000088 |
Nucleus | 0.001685 | 0.000504 | 0.000288 | 0.002209 | 0.001386 | 0.000121 | 0.000069 | 0.000602 | 0.001420 | 0.000328 | 0.000111 | 0.001286 |
Peroxisomes | 0.000382 | 0.000115 | 0.000006 | 0.000577 | 0.000105 | 0.000152 | 0.000008 | 0.000085 | 0.000137 | 0.000132 | 0.000008 | 0.000294 |
Vacuole | 0.069161 | 0.003344 | 0.082087 | 0.080945 | 0.004072 | 0.002574 | 0.021191 | 0.031419 | 0.011552 | 0.002991 | 0.032875 | 0.052514 |
Vacuole Periphery | 0.016122 | 0.001516 | 0.005345 | 0.010124 | 0.007870 | 0.001661 | 0.005616 | 0.005327 | 0.008819 | 0.001582 | 0.005564 | 0.007371 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.32 | 2.59 | 0.97 | -2.81 | -3.57 | 1.20 | 3.32 | 2.20 | 0.86 | -4.10 | 2.18 | 3.92 | 1.12 | -1.63 | -5.11 |
Bud Neck | 5.46 | -1.98 | -5.35 | -12.16 | 0.08 | -0.16 | -2.57 | -6.56 | -6.48 | -2.41 | 4.79 | -3.36 | -12.10 | -13.56 | -3.49 |
Bud Site | 4.72 | 4.53 | -0.00 | -11.57 | -10.69 | 0.26 | 2.59 | -3.58 | -3.48 | -5.56 | 4.47 | 4.94 | -7.25 | -11.91 | -12.22 |
Cell Periphery | 4.83 | -2.01 | 1.10 | -11.01 | 3.23 | 4.28 | -2.99 | -8.15 | -11.98 | -3.71 | 6.78 | -4.17 | -7.49 | -16.39 | -0.95 |
Cytoplasm | 3.96 | 4.00 | 0.26 | -10.88 | -11.01 | 2.16 | 3.00 | -2.59 | -5.65 | -6.61 | 4.34 | 4.81 | -5.28 | -12.15 | -12.71 |
Cytoplasmic Foci | 3.64 | 3.76 | -0.40 | -10.21 | -10.57 | 0.87 | 4.55 | -0.68 | -1.41 | -5.08 | 3.91 | 5.82 | -5.04 | -9.45 | -11.48 |
Eisosomes | 2.92 | -3.53 | 1.88 | -2.63 | 4.46 | 2.97 | -1.56 | 0.49 | -5.35 | 1.73 | 3.35 | -2.11 | 1.24 | -6.02 | 2.53 |
Endoplasmic Reticulum | -11.95 | -1.45 | 6.05 | 43.58* | 7.26 | -5.01 | 2.89 | 15.26 | 19.04 | 8.80 | -11.16 | 3.01 | 26.84 | 40.46* | 16.94 |
Endosome | 6.21 | 3.00 | -2.82 | -21.03 | -8.85 | -1.99 | 1.09 | -9.15 | -5.89 | -9.20 | 3.16 | 1.54 | -16.22 | -19.51 | -16.93 |
Golgi | 2.69 | 1.42 | -4.31 | -12.31 | -9.85 | 4.75 | -2.77 | -7.48 | -11.84 | -2.93 | 4.96 | -2.10 | -11.45 | -16.04 | -8.53 |
Lipid Particles | 5.24 | -0.00 | -6.89 | -20.24 | -4.70 | -0.59 | -2.68 | -12.18 | -11.96 | -7.40 | 3.84 | -2.49 | -19.89 | -22.60 | -12.14 |
Mitochondria | 1.32 | 1.43 | 0.00 | -4.35 | -4.78 | 4.07 | 5.92 | 4.86 | 0.44 | -3.59 | 5.06 | 6.07 | 3.76 | -2.83 | -5.96 |
Mitotic Spindle | 1.77 | 1.75 | -0.81 | -6.92 | -6.86 | -1.09 | 1.56 | -3.59 | -2.63 | -3.99 | 1.64 | 1.89 | -5.17 | -7.37 | -7.57 |
None | -2.21 | 2.74 | -4.59 | -4.11 | -4.68 | 4.14 | 6.34 | -2.05 | -3.27 | -3.76 | 1.42 | 6.52 | -4.64 | -4.97 | -5.78 |
Nuclear Periphery | 3.15 | -5.52 | -6.67 | -8.80 | 4.59 | 0.86 | -7.85 | -14.55 | -12.49 | 2.24 | 2.47 | -8.41 | -14.33 | -14.74 | 4.55 |
Nuclear Periphery Foci | 4.47 | -0.21 | -7.12 | -15.81 | -6.18 | -1.32 | -1.88 | -8.73 | -7.73 | -7.02 | 2.51 | -2.28 | -15.76 | -17.22 | -12.53 |
Nucleolus | -0.98 | 0.93 | -2.30 | 0.35 | -5.81 | 0.81 | 1.87 | 1.26 | 0.41 | -3.55 | -0.62 | 1.96 | -0.22 | 0.57 | -6.81 |
Nucleus | 2.55 | 3.02 | -0.77 | -5.45 | -7.42 | 1.79 | 1.85 | 0.84 | -3.05 | -3.31 | 1.81 | 2.20 | -0.35 | -5.53 | -8.26 |
Peroxisomes | 2.40 | 3.76 | -0.66 | -1.75 | -2.23 | -0.71 | 8.24 | 1.37 | 1.00 | -7.65 | 0.03 | 8.31 | -1.39 | -1.32 | -2.65 |
Vacuole | 6.49 | -0.57 | -1.24 | -21.53 | -0.43 | 1.81 | -5.93 | -12.79 | -13.85 | -3.13 | 5.87 | -5.84 | -17.10 | -24.66 | -5.70 |
Vacuole Periphery | 2.76 | 1.99 | 1.10 | -10.93 | -3.62 | 5.88 | 1.29 | 2.39 | -5.17 | 0.26 | 6.89 | 2.08 | 1.30 | -11.27 | -1.43 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|