Standard name
Human Ortholog
Description Alpha subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0.07 0.11
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.11 0.45 0.42 0 0.1 0.08 0.07 0.05 0.1
Mitochondria 0.93 0.96 0.95 0.94 0.89 0.91 0.88 0.85 0.93 0.94 0.94 0.92 0.98 0.89 0.92 0.17 0.17 0.12 0.87 0.74 0.79 0.8 0.7 0.62
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.05 0.06 0.06 0.12 0.08 0.07 0.07 0.05 0 0 0.06 0 0.08 0 0 0 0 0 0
Nucleolus 0.09 0.06 0.05 0.07 0.11 0.14 0.13 0.14 0.19 0.11 0.09 0.11 0.06 0 0 0.17 0.14 0.1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.25 0.07 0 0 0 0.06 0.08 0.11 0.09
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.14 0.1 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 2 5 0 1 1 1 2 0 2 1 1 4 2 1 2 3 7 4 5 2 14 37
Bud 2 0 0 1 6 6 7 8 8 8 4 9 1 0 3 2 0 2 1 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 2 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 0 2 3 2 2 1 1 2 1 0 1 3 3 0 4 3 0 1 0 0 0 0
Cytoplasm 2 0 1 7 12 11 13 15 8 4 5 8 0 6 4 5 1 1 0 0 0 0 0 0
Endoplasmic Reticulum 0 2 2 0 1 1 0 1 3 0 1 1 0 1 0 2 2 0 0 0 0 0 0 1
Endosome 0 0 0 0 6 2 2 3 1 1 0 0 0 0 0 0 0 1 0 1 1 0 5 2
Golgi 1 0 2 6 4 1 1 0 1 0 0 2 0 9 16 4 19 22 11 10 15 5 9 31
Mitochondria 241 96 171 189 242 257 359 406 275 364 301 312 64 145 217 6 7 6 228 77 144 70 149 203
Nucleus 0 0 0 0 0 1 3 2 1 1 1 1 0 0 1 0 0 1 0 0 0 0 0 0
Nuclear Periphery 7 4 8 5 7 13 25 31 36 29 22 24 3 5 7 2 1 4 1 0 0 0 0 1
Nucleolus 22 6 9 14 30 40 51 67 56 41 30 37 4 7 7 6 6 5 1 2 0 0 1 1
Peroxisomes 4 0 2 6 2 4 10 11 2 7 3 2 1 4 7 9 3 2 8 1 11 6 23 29
SpindlePole 0 0 0 1 2 2 3 6 0 5 0 3 0 0 0 0 0 1 0 0 0 0 1 0
Vac/Vac Membrane 16 7 7 5 8 1 12 11 8 8 6 9 3 11 14 5 4 8 1 3 2 0 1 6
Unique Cell Count 258 100 180 201 273 281 407 479 297 386 321 338 65 163 236 36 42 52 262 104 184 88 212 326
Labelled Cell Count 298 118 204 241 323 342 489 564 402 472 376 412 78 195 281 42 49 59 262 104 184 88 212 326


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.9 16.3 15.0 17.7 17.1 14.4 14.0 12.9 12.6 11.9 11.3 12.4 18.8 19.5 18.2 16.1 18.6 20.7 17.5 17.3 18.5
Std Deviation (1e-4) 4.4 3.9 4.4 5.1 4.5 4.1 3.8 3.9 3.7 3.5 3.0 3.7 4.3 9.5 4.0 4.7 6.2 5.6 5.0 4.6 4.8
Intensity Change (Log2) – – – 0.24 0.19 -0.06 -0.1 -0.22 -0.25 -0.33 -0.41 -0.27 0.32 0.38 0.28 0.1 0.31 0.46 0.22 0.21 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 2.3 2.8 0 0 8.6
Mitochondria -0.4 -2.3 -1.4 -2.6 -3.5 -1.0 -0.3 -0.6 -1.2 1.2 -2.1 -1.2 -11.2 -11.5 -12.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.1 -1.1 0.1 0.8 1.0 2.8 1.4 1.1 1.2 0 -0.7 -0.8 0 0 0
Nucleolus 0.8 2.2 3.1 2.8 3.2 4.3 2.2 1.7 2.3 0 -0.3 -1.1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.8 -0.6 0 -0.6 0 -0.7 0 0 -0.8 0 1.2 0.9 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 25.1233 26.6518 25.9525 24.7636 23.2103 26.1643 23.641 26.0834 23.0875 23.736 21.6066 23.6813 27.3441 30.1366 28.1674 24.9279 22.2337 26.479
Actin 0.0112 0.0005 0.003 0.0001 0.0145 0.0106 0.0335 0.0013 0.0023 0.0005 0.0087 0.0002 0.0095 0.0056 0.009 0.0007 0.0229 0.0004
Bud 0.0002 0 0.0001 0 0.0005 0 0.0001 0 0.0002 0 0.0006 0 0.0002 0.0001 0 0.0001 0.0002 0
Bud Neck 0.0001 0 0.0001 0 0.0027 0.0001 0.001 0 0 0 0.0004 0 0.0015 0.0002 0.0001 0 0.0001 0
Bud Periphery 0.0003 0 0.0002 0 0.0006 0.0001 0.0002 0.0001 0.0004 0 0.0007 0 0.0003 0.0001 0.0001 0.0001 0.0002 0
Bud Site 0.0003 0 0.0004 0 0.0073 0.0001 0.001 0.0001 0.0013 0 0.005 0 0.003 0.0003 0.0025 0.0003 0.0005 0
Cell Periphery 0.0002 0.0001 0.0003 0 0.0002 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001
Cytoplasm 0.0005 0 0.0001 0 0.0004 0 0.0001 0 0 0 0.0008 0 0.0046 0 0.0005 0.0004 0.0009 0
Cytoplasmic Foci 0.0007 0.0001 0.0007 0 0.0013 0.0016 0.0069 0.0002 0.0001 0 0.047 0.0001 0.0188 0.0005 0.0063 0.0198 0.0317 0.0002
Eisosomes 0.0007 0.0002 0.0008 0.0001 0.0002 0.0002 0.0003 0.0003 0.0002 0.0001 0.0003 0.0001 0.0003 0.0003 0.0002 0.0004 0.0008 0.0001
Endoplasmic Reticulum 0.0004 0 0.0004 0 0.0005 0.0001 0 0 0 0 0.0002 0 0.0012 0 0.0001 0.0001 0.0007 0
Endosome 0.0049 0.0005 0.0005 0 0.0035 0.0011 0.0008 0.0007 0.0001 0.0001 0.0032 0.0003 0.0043 0.0004 0.0006 0.0004 0.0096 0.0004
Golgi 0.0021 0.0016 0.0009 0.0001 0.0027 0.0031 0.0024 0.0036 0.0003 0.0001 0.0062 0.0009 0.0025 0.01 0.0045 0.0005 0.006 0.0055
Lipid Particles 0.0054 0.0038 0.0033 0.0015 0.0024 0.0062 0.0113 0.0054 0.0008 0.0022 0.0551 0.0113 0.0229 0.0044 0.0094 0.0283 0.0459 0.0124
Mitochondria 0.947 0.983 0.9758 0.9949 0.95 0.9509 0.9009 0.9781 0.9871 0.9955 0.8131 0.9659 0.8964 0.9705 0.9497 0.9227 0.8015 0.966
None 0.0013 0 0.0005 0 0.0003 0 0.0001 0 0 0 0.0038 0 0.0082 0 0.0052 0.0024 0.0079 0
Nuclear Periphery 0.0023 0 0.0002 0 0.0009 0.0005 0.0001 0 0 0 0.0033 0.0004 0.0068 0 0.0004 0.0009 0.006 0
Nucleolus 0.0026 0.0001 0.0002 0.0001 0.0004 0.0001 0.016 0.0001 0.0001 0 0.0064 0.0001 0.0012 0.0003 0.0011 0.0079 0.0088 0.0001
Nucleus 0.0055 0 0.0001 0 0.0008 0.0001 0.0012 0 0.0001 0 0.0014 0.0001 0.0014 0 0.0007 0.0016 0.0012 0
Peroxisomes 0.0017 0.001 0.0051 0.0002 0.0008 0.004 0.0196 0.0029 0.0022 0.0006 0.0266 0.0003 0.0048 0.0045 0.0017 0.0034 0.0124 0.0012
Punctate Nuclear 0.0033 0 0.0001 0 0.0021 0.0001 0.0004 0 0 0 0.0121 0 0.0075 0 0.0038 0.0024 0.0066 0
Vacuole 0.0018 0.0011 0.0017 0.0002 0.0015 0.0012 0.0004 0.0008 0.0014 0.0001 0.0007 0.0008 0.0011 0.0002 0.0003 0.0008 0.0023 0.0005
Vacuole Periphery 0.0075 0.0077 0.0055 0.0028 0.0061 0.0199 0.0036 0.0064 0.003 0.0005 0.0042 0.0195 0.0034 0.0025 0.0038 0.0066 0.0334 0.0131

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.8822 30.6328 44.4515 43.2031 30.113 46.4254 48.1799 45.7577 56.4999 51.0109
Translational Efficiency 0.8544 1.0443 0.7089 0.933 1.0504 1.0822 0.8793 0.8884 0.8619 0.8726

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2237 1480 165 1399 1137 1490 175 417 3374 2970 340 1816

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1083.62 1716.92 2465.11 1645.56 1609.44 1589.27 2298.88 1590.06 1260.82 1652.88 2379.55 1632.82
Standard Deviation 231.85 429.25 440.35 330.13 349.23 405.28 387.74 409.24 372.17 422.25 422.36 350.66
Intensity Change Log 2 — 0.663964 1.185793 0.602720 — -0.018195 0.514372 -0.017478 — 0.295923 0.822924 0.264796

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001354 0.008024 0.006128 0.005460 0.001995 0.007142 0.007129 0.005558 0.001570 0.007581 0.006643 0.005483
Bud Neck 0.001256 0.001615 0.013681 0.002174 0.001050 0.001273 0.007856 0.006675 0.001187 0.001444 0.010683 0.003208
Bud Site 0.003507 0.001513 0.010396 0.002385 0.000892 0.000931 0.007044 0.019981 0.002626 0.001221 0.008671 0.006426
Cell Periphery 0.000415 0.000068 0.000276 0.000208 0.000060 0.000099 0.000174 0.002537 0.000295 0.000084 0.000223 0.000743
Cytoplasm 0.000437 0.000263 0.000661 0.000290 0.000328 0.000365 0.000522 0.001374 0.000400 0.000314 0.000590 0.000539
Cytoplasmic Foci 0.005675 0.018299 0.110569 0.008569 0.025207 0.017433 0.075695 0.011969 0.012257 0.017865 0.092619 0.009349
Eisosomes 0.000291 0.002025 0.002117 0.000512 0.001167 0.001704 0.001142 0.000498 0.000586 0.001864 0.001615 0.000509
Endoplasmic Reticulum 0.000127 0.000109 0.000205 0.000430 0.000088 0.000116 0.000226 0.001314 0.000114 0.000113 0.000216 0.000633
Endosome 0.002350 0.002036 0.001814 0.004408 0.000926 0.002178 0.002949 0.021061 0.001870 0.002107 0.002398 0.008232
Golgi 0.005180 0.023651 0.014389 0.030355 0.010736 0.025356 0.028995 0.059604 0.007052 0.024507 0.021907 0.037071
Lipid Particles 0.003186 0.005723 0.010994 0.007310 0.006039 0.006817 0.013468 0.006387 0.004148 0.006272 0.012268 0.007098
Mitochondria 0.951001* 0.909734* 0.719688* 0.895421* 0.924586* 0.911730* 0.763001* 0.776772* 0.942100* 0.910735* 0.741981* 0.868176*
Mitotic Spindle 0.000474 0.000642 0.019649 0.004530 0.000490 0.000932 0.018890 0.003309 0.000479 0.000787 0.019259 0.004249
None 0.001571 0.002274 0.003953 0.002505 0.003479 0.002359 0.003793 0.003176 0.002214 0.002317 0.003871 0.002659
Nuclear Periphery 0.000124 0.000156 0.005264 0.001416 0.000365 0.000204 0.004001 0.001441 0.000206 0.000180 0.004614 0.001421
Nuclear Periphery Foci 0.000141 0.000176 0.001669 0.000532 0.000271 0.000196 0.001823 0.000743 0.000185 0.000186 0.001748 0.000580
Nucleolus 0.000537 0.000662 0.006857 0.000586 0.000977 0.000387 0.002557 0.000591 0.000685 0.000524 0.004644 0.000587
Nucleus 0.000195 0.000063 0.000361 0.000579 0.000216 0.000147 0.000275 0.000275 0.000202 0.000105 0.000317 0.000510
Peroxisomes 0.012037 0.017037 0.034865 0.004158 0.012351 0.014025 0.017508 0.007028 0.012143 0.015526 0.025932 0.004817
Vacuole 0.004659 0.003110 0.016155 0.008568 0.003532 0.003093 0.018274 0.031949 0.004279 0.003102 0.017246 0.013937
Vacuole Periphery 0.005481 0.002818 0.020308 0.019603 0.005247 0.003515 0.024678 0.037759 0.005402 0.003168 0.022557 0.023772

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.99 -4.13 -4.38 1.80 0.45 -4.86 -3.15 -2.13 0.84 0.70 -7.88 -5.06 -4.78 1.94 0.92
Bud Neck -1.00 -5.16 -1.86 -1.05 4.70 -0.80 -5.21 -3.45 -3.31 0.59 -1.10 -7.01 -3.78 -3.24 5.17
Bud Site 2.29 -1.49 1.28 -1.30 1.73 -0.05 -1.53 -4.12 -4.12 -1.89 2.58 -1.99 -3.13 -4.49 0.77
Cell Periphery 2.74 0.72 1.60 -5.19 0.44 -2.03 -2.23 -3.79 -3.73 -3.59 2.52 0.59 -2.58 -4.31 -3.02
Cytoplasm 1.14 -0.94 0.93 -0.54 1.93 -0.31 -1.23 -2.14 -2.05 -1.66 0.72 -1.26 -0.91 -1.76 0.35
Cytoplasmic Foci -8.14 -7.45 -2.60 5.72 7.23 2.84 -5.13 4.05 2.00 6.47 -4.12 -9.45 2.24 6.31 9.78
Eisosomes -10.28 -5.66 -2.69 8.16 4.84 -3.26 0.14 5.32 8.00 3.75 -11.37 -5.69 1.05 10.95 5.88
Endoplasmic Reticulum 0.85 -1.34 -2.99 -3.18 -1.98 -1.60 -2.64 -3.65 -3.57 -3.20 0.09 -2.59 -4.72 -4.75 -3.61
Endosome 0.56 1.17 -3.26 -3.30 -4.03 -3.30 -4.01 -5.65 -5.27 -5.04 -0.66 -1.47 -6.63 -6.25 -5.94
Golgi -8.60 -3.15 -8.79 -1.96 -4.04 -5.70 -3.51 -6.09 -4.18 -3.28 -10.74 -4.92 -10.37 -3.95 -3.73
Lipid Particles -2.99 -3.56 -3.22 -1.20 1.51 -0.67 -2.99 -0.18 0.20 2.33 -3.06 -4.92 -2.70 -0.74 2.74
Mitochondria 6.89 8.91 8.41 1.92 -6.64 1.73 7.24 9.01 8.27 -0.54 6.89 11.77 11.44 6.26 -7.10
Mitotic Spindle -0.74 -3.67 -3.69 -3.49 2.79 -0.85 -2.98 -3.53 -2.62 2.49 -1.11 -4.63 -4.33 -3.84 3.56
None -4.15 -5.52 -5.62 -1.18 3.27 4.50 -0.73 0.35 -0.94 0.66 -0.76 -5.61 -1.84 -1.39 3.38
Nuclear Periphery -1.43 -3.41 -4.99 -4.86 2.52 2.46 -3.76 -2.23 -2.56 2.37 0.83 -4.99 -5.32 -5.42 3.50
Nuclear Periphery Foci -0.89 -5.01 -3.79 -3.36 3.53 1.31 -3.03 -3.57 -4.05 2.05 -0.03 -5.19 -4.64 -4.50 3.73
Nucleolus -0.50 -2.91 -0.21 0.34 2.89 1.83 -2.47 1.10 -1.30 3.38 0.83 -3.57 0.42 -0.48 3.68
Nucleus 1.31 -1.37 -0.78 -1.07 -0.44 0.38 -0.45 -0.43 -0.94 0.01 0.98 -1.28 -0.81 -1.06 -0.51
Peroxisomes -2.09 -3.50 4.85 6.12 4.76 -0.70 -1.26 2.37 3.20 2.62 -2.02 -3.61 5.61 7.35 5.65
Vacuole 2.09 -5.49 -3.38 -4.90 3.34 0.58 -4.86 -6.48 -6.60 -2.61 2.17 -6.95 -7.13 -8.16 1.46
Vacuole Periphery 3.27 -3.20 -7.31 -9.21 0.14 1.95 -3.54 -7.39 -7.86 -1.89 3.59 -4.75 -10.25 -11.89 -0.32
Standard name
Human Ortholog
Description Alpha subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Mas2

Mas2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mas2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available