Standard name | |
---|---|
Human Ortholog | |
Description | Homoserine kinase; conserved protein required for threonine biosynthesis; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic filaments; expression is regulated by the GCN4-mediated general amino acid control pathway |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 0 | 0 | 0 | 1 | 4 | 5 | 8 | 7 | 7 | 14 | 1 | 2 | 0 | 1 | 5 | 2 | 0 | 3 | 1 | 0 | 2 | 1 |
Bud | 4 | 2 | 0 | 1 | 2 | 0 | 6 | 3 | 12 | 6 | 13 | 15 | 2 | 0 | 0 | 1 | 0 | 3 | 0 | 7 | 2 | 3 | 5 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 1 | 4 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 4 | 1 | 2 | 1 | 6 | 7 | 10 | 11 | 10 | 6 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 131 | 131 | 217 | 152 | 257 | 211 | 219 | 176 | 155 | 117 | 97 | 94 | 70 | 22 | 3 | 113 | 152 | 160 | 154 | 160 | 206 | 89 | 170 | 173 |
Endoplasmic Reticulum | 0 | 4 | 1 | 0 | 9 | 4 | 3 | 9 | 7 | 4 | 4 | 2 | 1 | 0 | 0 | 6 | 5 | 4 | 2 | 0 | 2 | 0 | 1 | 4 |
Endosome | 1 | 0 | 0 | 0 | 0 | 3 | 2 | 4 | 5 | 6 | 5 | 12 | 1 | 2 | 5 | 0 | 0 | 1 | 2 | 4 | 1 | 0 | 4 | 4 |
Golgi | 1 | 11 | 1 | 0 | 0 | 1 | 6 | 4 | 5 | 5 | 3 | 7 | 2 | 16 | 3 | 19 | 34 | 32 | 1 | 3 | 0 | 1 | 1 | 0 |
Mitochondria | 4 | 2 | 0 | 0 | 0 | 1 | 10 | 9 | 29 | 18 | 44 | 19 | 1 | 1 | 4 | 7 | 4 | 16 | 1 | 0 | 1 | 0 | 0 | 1 |
Nucleus | 2 | 0 | 2 | 1 | 0 | 1 | 6 | 0 | 0 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 9 | 10 | 4 | 0 | 0 | 5 | 2 | 12 | 13 | 17 | 18 | 28 | 18 | 39 | 23 | 54 | 52 | 46 | 1 | 4 | 0 | 1 | 2 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 5 | 3 | 6 | 8 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 2 |
SpindlePole | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 4 | 3 | 9 | 3 | 20 | 5 | 20 | 11 | 8 | 11 | 8 | 7 | 11 | 6 | 2 | 21 | 27 |
Vac/Vac Membrane | 8 | 13 | 3 | 11 | 6 | 40 | 65 | 90 | 91 | 72 | 55 | 103 | 5 | 5 | 2 | 13 | 28 | 21 | 0 | 5 | 2 | 0 | 5 | 4 |
Unique Cell Count | 155 | 170 | 227 | 164 | 271 | 262 | 315 | 301 | 299 | 244 | 225 | 281 | 98 | 97 | 44 | 205 | 265 | 268 | 183 | 209 | 230 | 108 | 233 | 250 |
Labelled Cell Count | 163 | 179 | 233 | 166 | 276 | 270 | 330 | 325 | 338 | 277 | 264 | 330 | 110 | 113 | 59 | 226 | 294 | 293 | 183 | 209 | 230 | 108 | 233 | 250 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 32.4 | 43.6 | 32.2 | 28.9 | 26.6 | 20.8 | 17.9 | 18.8 | 16.5 | 16.7 | 14.6 | 15.9 | 33.7 | 35.0 | 35.1 | 45.7 | 47.8 | 46.8 | 34.4 | 34.0 | 34.3 |
Std Deviation (1e-4) | 8.0 | 9.4 | 7.2 | 7.4 | 5.0 | 4.8 | 4.3 | 3.8 | 4.2 | 4.1 | 4.4 | 4.1 | 8.2 | 8.4 | 12.6 | 15.2 | 14.1 | 14.6 | 9.2 | 8.9 | 7.8 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.27 | -0.63 | -0.85 | -0.77 | -0.97 | -0.95 | -1.14 | -1.02 | 0.06 | 0.12 | 0.12 | 0.5 | 0.57 | 0.54 | 0.1 | 0.08 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 3.7 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1.1 | 1.7 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.2 | -0.4 | -5.0 | -7.5 | -9.7 | -10.9 | -11.4 | -12.1 | -14.3 | -6.2 | -13.7 | -13.8 | -9.9 | -9.8 | -9.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.9 | 0 | 4.4 | 5.3 | 5.1 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.8 | 4.2 | 7.0 | 4.0 | 0 | 0 | 0 | 0 | 0 | 3.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1.5 | 1.7 | 2.7 | 3.1 | 3.8 | 5.5 | 9.3 | 0 | 7.5 | 6.2 | 5.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 0 | 6.8 | 0 | 0 | 2.7 | 0 |
Vacuole | 2.8 | 0 | 5.4 | 6.7 | 8.5 | 8.6 | 8.4 | 7.3 | 9.7 | 0 | 0 | 0 | 2.8 | 4.2 | 3.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 27.0975 | 39.3788 | 31.3542 | 35.3176 | 26.8745 | 37.2329 | 28.6842 | 40.3807 | 32.9414 | 28.6885 | 30.4227 | 30.237 | 23.3333 | 36.856 | 35.6447 | 28.4563 | 34.5903 | 28.7473 |
Actin | 0.0153 | 0.001 | 0.0057 | 0 | 0.0478 | 0.0004 | 0.0151 | 0.005 | 0.0092 | 0.0106 | 0.0004 | 0.0072 | 0.0105 | 0.0057 | 0.003 | 0.0265 | 0.0014 | 0.0007 |
Bud | 0.0019 | 0.004 | 0.0018 | 0 | 0.0049 | 0.0005 | 0.0036 | 0.0103 | 0.0013 | 0.0008 | 0.0007 | 0.0038 | 0.0016 | 0.0228 | 0.001 | 0.0186 | 0.0026 | 0.0009 |
Bud Neck | 0.0003 | 0.0174 | 0.0008 | 0 | 0.0009 | 0.0013 | 0.0029 | 0.0074 | 0.0032 | 0.0009 | 0.0005 | 0.0015 | 0.001 | 0.0027 | 0.0009 | 0.0035 | 0.0009 | 0.0008 |
Bud Periphery | 0.0014 | 0.0012 | 0.0007 | 0 | 0.0025 | 0.0002 | 0.0015 | 0.0031 | 0.0004 | 0.0007 | 0.0001 | 0.0036 | 0.0017 | 0.0092 | 0.0007 | 0.0269 | 0.0008 | 0.0003 |
Bud Site | 0.0018 | 0.0173 | 0.0067 | 0 | 0.0121 | 0.0001 | 0.0047 | 0.0285 | 0.0051 | 0.0088 | 0.0005 | 0.0029 | 0.0145 | 0.0517 | 0.0019 | 0.0118 | 0.0004 | 0.0007 |
Cell Periphery | 0.0003 | 0.001 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0.0007 | 0.0002 | 0.0007 | 0.0038 | 0.0006 | 0.0007 | 0.0037 | 0.0008 | 0.0007 | 0.0009 | 0.0004 |
Cytoplasm | 0.5064 | 0.7279 | 0.6917 | 0.9664 | 0.6794 | 0.5656 | 0.6978 | 0.7473 | 0.721 | 0.6615 | 0.7211 | 0.5284 | 0.5949 | 0.6751 | 0.7858 | 0.8042 | 0.7167 | 0.7923 |
Cytoplasmic Foci | 0.3041 | 0.1207 | 0.1303 | 0 | 0.109 | 0.2637 | 0.1277 | 0.1021 | 0.1677 | 0.0304 | 0.0977 | 0.2145 | 0.1086 | 0.1386 | 0.1329 | 0.0077 | 0.158 | 0.0781 |
Eisosomes | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0068 | 0.0002 | 0.0199 | 0 | 0.0012 | 0.004 | 0.0069 | 0.0001 | 0.0027 | 0.0031 | 0.0012 | 0.0022 | 0.0282 | 0.0002 | 0.0019 | 0.0018 | 0.0006 | 0.0037 |
Endosome | 0.0199 | 0.0027 | 0.0191 | 0 | 0.0102 | 0.0076 | 0.0117 | 0.001 | 0.0093 | 0.0454 | 0.0018 | 0.0267 | 0.0333 | 0.0015 | 0.0015 | 0.0221 | 0.0026 | 0.0013 |
Golgi | 0.0065 | 0.0008 | 0.0035 | 0 | 0.004 | 0.0009 | 0.0039 | 0.0005 | 0.0022 | 0.0145 | 0.0004 | 0.0156 | 0.008 | 0.0008 | 0.0005 | 0.0075 | 0.0006 | 0.0001 |
Lipid Particles | 0.0131 | 0.0016 | 0.0153 | 0 | 0.009 | 0.0032 | 0.0049 | 0.0018 | 0.0091 | 0.0421 | 0.0188 | 0.0207 | 0.0071 | 0.0031 | 0.0015 | 0.001 | 0.0099 | 0.0029 |
Mitochondria | 0.015 | 0.006 | 0.0081 | 0.0001 | 0.0063 | 0.0014 | 0.0165 | 0.0055 | 0.0034 | 0.0148 | 0.0346 | 0.0514 | 0.0037 | 0.0106 | 0.0042 | 0.0145 | 0.0087 | 0.003 |
None | 0.0184 | 0.0019 | 0.0026 | 0.0001 | 0.0012 | 0.0006 | 0.005 | 0.001 | 0.011 | 0.0025 | 0.0059 | 0.007 | 0.0128 | 0.004 | 0.0004 | 0.0021 | 0.0038 | 0.0604 |
Nuclear Periphery | 0.0137 | 0.0015 | 0.0121 | 0.0009 | 0.0212 | 0.0508 | 0.0209 | 0.0013 | 0.0046 | 0.0135 | 0.0076 | 0.0111 | 0.0442 | 0.0013 | 0.003 | 0.0025 | 0.0044 | 0.0044 |
Nucleolus | 0.003 | 0.0464 | 0.0266 | 0 | 0.0014 | 0.0008 | 0.0043 | 0.0456 | 0.0103 | 0.0964 | 0.015 | 0.0439 | 0.0011 | 0.0245 | 0.0352 | 0.0007 | 0.0218 | 0.0312 |
Nucleus | 0.0395 | 0.0275 | 0.0367 | 0.0324 | 0.0617 | 0.06 | 0.0325 | 0.0151 | 0.0126 | 0.0246 | 0.0271 | 0.0207 | 0.1014 | 0.0109 | 0.0143 | 0.0143 | 0.0189 | 0.0116 |
Peroxisomes | 0.0143 | 0.0057 | 0.0033 | 0 | 0.0169 | 0.0035 | 0.0068 | 0.0031 | 0.0039 | 0.0052 | 0.0221 | 0.0135 | 0.0031 | 0.0035 | 0.0039 | 0.0024 | 0.0097 | 0.0019 |
Punctate Nuclear | 0.0099 | 0.0068 | 0.0094 | 0 | 0.0022 | 0.0124 | 0.0293 | 0.0177 | 0.0204 | 0.0077 | 0.0085 | 0.0131 | 0.0139 | 0.0081 | 0.0025 | 0.0007 | 0.0033 | 0.001 |
Vacuole | 0.0053 | 0.0057 | 0.0033 | 0 | 0.0051 | 0.0133 | 0.0022 | 0.0022 | 0.0015 | 0.0082 | 0.0274 | 0.0062 | 0.0068 | 0.0171 | 0.0021 | 0.0274 | 0.0254 | 0.0032 |
Vacuole Periphery | 0.0032 | 0.0025 | 0.0022 | 0 | 0.0024 | 0.0096 | 0.0014 | 0.0005 | 0.0008 | 0.0073 | 0.0048 | 0.0054 | 0.0028 | 0.0048 | 0.0019 | 0.0031 | 0.0086 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 233.7286 | 247.0532 | 222.8513 | 203.4311 | 195.144 | 221.7909 | 240.2771 | 201.4992 | 214.2878 | 184.9452 |
Translational Efficiency | 1.0589 | 1.05 | 1.1193 | 1.2648 | 1.0811 | 1.1983 | 1.146 | 1.2536 | 1.0987 | 1.1155 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
181 | 1860 | 1296 | 1072 | 1708 | 1571 | 595 | 1301 | 1889 | 3431 | 1891 | 2373 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2282.04 | 2904.38 | 2394.44 | 2463.80 | 2531.97 | 2851.72 | 2595.93 | 2590.54 | 2508.02 | 2880.27 | 2457.84 | 2533.29 |
Standard Deviation | 435.19 | 560.06 | 865.73 | 886.04 | 443.88 | 557.77 | 833.84 | 872.77 | 449.12 | 559.63 | 860.92 | 881.05 |
Intensity Change Log 2 | — | 0.347906 | 0.069364 | 0.110561 | — | 0.171572 | 0.035991 | 0.032993 | — | 0.257853 | 0.051908 | 0.070284 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000644 | 0.000110 | 0.001519 | 0.003456 | 0.000237 | 0.000297 | 0.001578 | 0.001529 | 0.000276 | 0.000196 | 0.001537 | 0.002400 |
Bud Neck | 0.011341 | 0.001200 | 0.022593 | 0.029600 | 0.009041 | 0.001554 | 0.031129 | 0.027501 | 0.009261 | 0.001362 | 0.025279 | 0.028449 |
Bud Site | 0.057488 | 0.007089 | 0.049306 | 0.078832 | 0.044550 | 0.009410 | 0.088797 | 0.114046* | 0.045790 | 0.008152 | 0.061732 | 0.098138 |
Cell Periphery | 0.000725 | 0.000173 | 0.000391 | 0.000496 | 0.000435 | 0.000125 | 0.000647 | 0.000623 | 0.000463 | 0.000151 | 0.000472 | 0.000566 |
Cytoplasm | 0.570347* | 0.727720* | 0.008516 | 0.012431 | 0.571635* | 0.674639* | 0.016232 | 0.029921 | 0.571512* | 0.703415* | 0.010944 | 0.022020 |
Cytoplasmic Foci | 0.171100 | 0.020021 | 0.599222* | 0.511984* | 0.164037 | 0.029186 | 0.629327* | 0.517809* | 0.164713 | 0.024217 | 0.608695* | 0.515178* |
Eisosomes | 0.000036 | 0.000002 | 0.000821 | 0.000709 | 0.000015 | 0.000004 | 0.000369 | 0.000371 | 0.000017 | 0.000003 | 0.000679 | 0.000524 |
Endoplasmic Reticulum | 0.000418 | 0.000049 | 0.000186 | 0.000267 | 0.000149 | 0.000064 | 0.000165 | 0.000148 | 0.000175 | 0.000056 | 0.000179 | 0.000202 |
Endosome | 0.001855 | 0.000113 | 0.001814 | 0.003358 | 0.000579 | 0.000337 | 0.004170 | 0.004041 | 0.000702 | 0.000216 | 0.002555 | 0.003733 |
Golgi | 0.007934 | 0.000456 | 0.001405 | 0.003000 | 0.001331 | 0.000637 | 0.001834 | 0.003186 | 0.001963 | 0.000539 | 0.001540 | 0.003102 |
Lipid Particles | 0.001199 | 0.000645 | 0.004872 | 0.006161 | 0.000659 | 0.000284 | 0.004512 | 0.002792 | 0.000711 | 0.000480 | 0.004759 | 0.004314 |
Mitochondria | 0.001132 | 0.000145 | 0.014759 | 0.016725 | 0.000274 | 0.000142 | 0.007539 | 0.012296 | 0.000356 | 0.000144 | 0.012487 | 0.014297 |
Mitotic Spindle | 0.008292 | 0.001814 | 0.096940 | 0.113154* | 0.010555 | 0.007688 | 0.104827* | 0.137494* | 0.010339 | 0.004504 | 0.099422 | 0.126498* |
None | 0.004279 | 0.018514 | 0.000613 | 0.000593 | 0.009662 | 0.018410 | 0.000591 | 0.000793 | 0.009146 | 0.018466 | 0.000606 | 0.000703 |
Nuclear Periphery | 0.000179 | 0.000066 | 0.001287 | 0.001503 | 0.000200 | 0.000128 | 0.000955 | 0.001006 | 0.000198 | 0.000095 | 0.001182 | 0.001230 |
Nuclear Periphery Foci | 0.005632 | 0.000867 | 0.003346 | 0.002842 | 0.003246 | 0.001929 | 0.002766 | 0.002089 | 0.003475 | 0.001353 | 0.003164 | 0.002430 |
Nucleolus | 0.006655 | 0.000306 | 0.023167 | 0.026600 | 0.000895 | 0.000149 | 0.011145 | 0.017230 | 0.001447 | 0.000234 | 0.019384 | 0.021463 |
Nucleus | 0.123867 | 0.217516* | 0.008478 | 0.011685 | 0.172302* | 0.251111* | 0.011812 | 0.012238 | 0.167661* | 0.232899* | 0.009527 | 0.011988 |
Peroxisomes | 0.004240 | 0.000293 | 0.150309* | 0.163122* | 0.003038 | 0.000958 | 0.067991 | 0.103454* | 0.003153 | 0.000597 | 0.124407* | 0.130409* |
Vacuole | 0.014546 | 0.002728 | 0.008383 | 0.011204 | 0.006140 | 0.002836 | 0.010530 | 0.009634 | 0.006945 | 0.002777 | 0.009059 | 0.010343 |
Vacuole Periphery | 0.008091 | 0.000173 | 0.002073 | 0.002278 | 0.001019 | 0.000112 | 0.003083 | 0.001797 | 0.001697 | 0.000145 | 0.002391 | 0.002014 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.49 | -2.76 | -4.35 | -5.53 | -2.83 | -0.69 | -4.53 | -5.09 | -2.79 | 0.72 | 0.41 | -6.17 | -6.64 | -6.42 | -2.00 |
Bud Neck | 3.86 | -4.05 | -6.14 | -15.68 | -3.31 | 9.62 | -8.76 | -10.88 | -18.52 | 1.82 | 10.56 | -10.91 | -13.61 | -24.09 | -1.77 |
Bud Site | 4.15 | 0.27 | -2.52 | -16.24 | -6.13 | 10.84 | -6.75 | -12.67 | -21.87 | -3.17 | 12.27 | -4.28 | -12.54 | -27.20 | -8.72 |
Cell Periphery | 5.89 | 3.43 | 1.60 | -7.23 | -2.73 | 5.08 | -2.13 | -2.89 | -9.57 | -0.16 | 5.52 | 0.16 | -2.27 | -11.80 | -2.88 |
Cytoplasm | -5.44 | 21.46 | 21.06 | 138.73* | -4.36 | -10.51 | 64.06* | 60.06* | 95.31* | -5.77 | -15.76 | 72.05 | 67.56 | 158.70* | -9.18 |
Cytoplasmic Foci | 6.40 | -17.90 | -13.68 | -47.44* | 7.33 | 16.74 | -28.44 | -28.43 | -48.77 | 6.36 | 19.15 | -42.71 | -34.52 | -68.35* | 10.10 |
Eisosomes | 2.70 | -7.24 | -10.40 | -11.21 | 1.28 | 4.55 | -3.94 | -13.70 | -14.26 | 0.09 | 6.29 | -8.31 | -15.53 | -16.07 | 2.27 |
Endoplasmic Reticulum | 1.96 | 1.16 | 0.71 | -4.10 | -1.41 | 3.54 | -1.13 | 0.25 | -5.80 | 1.96 | 4.35 | -0.29 | -0.61 | -5.76 | -0.50 |
Endosome | 4.06 | -0.22 | -2.24 | -5.58 | -2.41 | 3.37 | -4.95 | -3.80 | -4.26 | 0.63 | 6.70 | -6.60 | -5.39 | -6.57 | -1.53 |
Golgi | 2.32 | 1.98 | 1.48 | -2.82 | -1.61 | 3.12 | -2.02 | -1.91 | -3.17 | -1.11 | 3.98 | 0.90 | -1.28 | -4.26 | -2.15 |
Lipid Particles | 1.69 | -3.92 | -4.51 | -5.21 | -0.96 | 3.35 | -2.97 | -6.39 | -8.56 | 1.42 | 1.53 | -5.63 | -7.05 | -7.44 | 0.63 |
Mitochondria | 1.54 | -7.11 | -7.01 | -7.75 | -0.43 | 6.77 | -5.07 | -7.57 | -7.69 | -1.85 | 3.74 | -9.02 | -10.65 | -10.86 | -0.41 |
Mitotic Spindle | 2.14 | -13.82 | -14.89 | -17.53 | -2.60 | 2.92 | -11.63 | -18.94 | -20.10 | -2.18 | 5.01 | -18.31 | -23.93 | -26.79 | -4.35 |
None | -11.21 | 3.77 | 3.74 | 22.42* | -0.34 | -7.54 | 16.44 | 15.66 | 19.04 | -2.15 | -11.37 | 16.93 | 16.49 | 29.30* | -2.10 |
Nuclear Periphery | 3.00 | -3.95 | -4.13 | -4.52 | -0.57 | 3.39 | -5.24 | -5.35 | -5.88 | 0.58 | 6.25 | -5.23 | -6.20 | -6.94 | -0.06 |
Nuclear Periphery Foci | 1.96 | 1.05 | 1.28 | -2.98 | 0.77 | 1.99 | 0.27 | 1.95 | -0.27 | 1.75 | 3.61 | 0.64 | 2.05 | -2.26 | 1.70 |
Nucleolus | 1.43 | -4.13 | -3.95 | -9.61 | -0.10 | 5.62 | -6.20 | -7.97 | -8.54 | -1.41 | 3.18 | -12.45 | -11.52 | -12.82 | 0.44 |
Nucleus | -8.27 | 11.05 | 10.32 | 48.36* | -3.13 | -13.43 | 31.98 | 32.72 | 49.74* | -0.79 | -13.52 | 36.75 | 34.93 | 69.15* | -3.65 |
Peroxisomes | 3.08 | -26.50* | -22.50* | -23.54* | 0.28 | 3.00 | -12.96 | -19.41 | -20.10 | -4.04 | 4.22 | -29.06* | -29.45* | -30.52* | 1.53 |
Vacuole | 2.95 | 1.87 | -0.13 | -6.64 | -4.02 | 0.94 | -2.72 | -3.85 | -5.13 | -0.23 | 3.18 | -0.80 | -4.37 | -8.60 | -3.79 |
Vacuole Periphery | 2.28 | 1.66 | 1.61 | -4.99 | -0.28 | 3.15 | -1.42 | -1.77 | -6.59 | 0.88 | 3.69 | -1.02 | -0.67 | -7.80 | 0.64 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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