Standard name | |
---|---|
Human Ortholog | |
Description | Serine/threonine MAP kinase; coordinates expression of all 19S regulatory particle assembly-chaperones (RACs) to control proteasome abundance; involved in regulating maintenance of cell wall integrity, cell cycle progression, nuclear mRNA retention in heat shock, septum assembly; required for mitophagy, pexophagy; affects recruitment of mitochondria to phagophore assembly site; plays role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 1 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 7 | 1 | 6 | 5 | 9 | 23 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 2 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 1 | 2 | 2 | 4 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 339 | 100 | 169 | 68 | 138 | 183 | 196 | 224 | 136 | 149 | 108 | 159 | 128 | 211 | 283 | 87 | 82 | 79 | 300 | 81 | 137 | 42 | 82 | 76 |
Endoplasmic Reticulum | 0 | 1 | 2 | 1 | 1 | 6 | 2 | 8 | 7 | 3 | 1 | 7 | 4 | 0 | 2 | 6 | 4 | 5 | 2 | 1 | 4 | 0 | 3 | 9 |
Endosome | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 3 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 2 | 1 | 13 | 1 | 4 | 3 | 5 | 7 |
Golgi | 0 | 1 | 1 | 2 | 4 | 0 | 3 | 3 | 1 | 0 | 3 | 4 | 0 | 0 | 0 | 2 | 3 | 2 | 2 | 1 | 1 | 0 | 2 | 0 |
Mitochondria | 6 | 1 | 2 | 0 | 3 | 7 | 23 | 36 | 33 | 68 | 47 | 80 | 0 | 2 | 1 | 4 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 |
Nucleus | 10 | 6 | 6 | 16 | 27 | 16 | 37 | 47 | 24 | 32 | 24 | 31 | 4 | 3 | 5 | 2 | 7 | 4 | 91 | 27 | 42 | 25 | 38 | 58 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 2 |
Nucleolus | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 2 | 7 | 5 |
Vac/Vac Membrane | 67 | 18 | 41 | 42 | 92 | 74 | 178 | 132 | 40 | 76 | 44 | 68 | 44 | 89 | 41 | 84 | 62 | 68 | 11 | 13 | 15 | 1 | 10 | 33 |
Unique Cell Count | 385 | 120 | 194 | 114 | 226 | 260 | 406 | 424 | 224 | 300 | 213 | 321 | 161 | 264 | 307 | 162 | 137 | 130 | 455 | 131 | 227 | 90 | 174 | 230 |
Labelled Cell Count | 422 | 129 | 222 | 131 | 266 | 286 | 447 | 459 | 246 | 340 | 235 | 357 | 181 | 307 | 333 | 189 | 163 | 160 | 455 | 131 | 227 | 90 | 174 | 230 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.4 | 10.5 | 9.7 | 9.9 | 11.7 | 11.3 | 10.7 | 11.2 | 11.2 | 10.1 | 10.5 | 11.0 | 10.3 | 10.4 | 10.4 | 13.2 | 14.6 | 17.5 | 9.2 | 12.3 | 16.2 |
Std Deviation (1e-4) | 1.2 | 1.5 | 1.7 | 1.6 | 2.4 | 2.3 | 2.4 | 2.3 | 2.4 | 2.8 | 2.5 | 2.6 | 1.3 | 1.6 | 1.8 | 5.0 | 5.2 | 7.1 | 2.6 | 3.2 | 5.3 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.27 | 0.22 | 0.13 | 0.2 | 0.2 | 0.05 | 0.11 | 0.17 | 0.08 | 0.1 | 0.09 | 0.44 | 0.59 | 0.85 | -0.08 | 0.34 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -5.5 | -6.0 | -4.2 | -9.1 | -8.2 | -6.1 | -8.5 | -7.9 | -8.6 | -1.9 | -2.0 | 1.9 | -7.0 | -5.7 | -5.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.7 | 3.6 | 5.0 | 6.7 | 6.5 | 7.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 3.6 | 3.4 | 1.5 | 2.7 | 3.3 | 3.0 | 3.1 | 3.2 | 2.8 | 0 | 0 | 0 | 0 | 0.9 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.0 | 4.3 | 1.8 | 5.4 | 2.6 | -0.8 | 1.1 | -0.1 | 0 | 1.4 | 3.0 | -2.3 | 6.0 | 4.7 | 5.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9007 | 5.8455 | 4.6944 | 4.3198 | 3.9091 | 4.611 | 5.5099 | 6.203 | 6.3235 | 5.4453 | 5.9085 | 5.9139 | 7.666 | 8.5895 | 7.9324 | 7.9109 | 7.0657 | 8.1059 |
Actin | 0.008 | 0.0002 | 0.0005 | 0.001 | 0.0003 | 0.0025 | 0.0489 | 0.0011 | 0.0034 | 0.0112 | 0.0117 | 0.001 | 0.0266 | 0.0014 | 0.0009 | 0.0007 | 0.0006 | 0.0041 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0006 | 0.0004 | 0.0003 | 0.0013 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
Bud Neck | 0.0008 | 0.0005 | 0.0007 | 0.0023 | 0.0021 | 0.0035 | 0.0086 | 0.0007 | 0.0009 | 0.0045 | 0.0017 | 0.0024 | 0.0029 | 0.0017 | 0.0009 | 0.0014 | 0.0016 | 0.0034 |
Bud Periphery | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0016 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0 | 0 | 0 | 0.0001 |
Bud Site | 0.0003 | 0.0006 | 0.0004 | 0.0015 | 0.0002 | 0.0002 | 0.0016 | 0.0017 | 0.0009 | 0.0173 | 0.001 | 0.0003 | 0.0007 | 0.0082 | 0.0005 | 0.0003 | 0.0001 | 0.0002 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0018 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4747 | 0.4615 | 0.4416 | 0.4933 | 0.5296 | 0.3667 | 0.4659 | 0.6773 | 0.5253 | 0.6883 | 0.6695 | 0.5262 | 0.4864 | 0.5968 | 0.551 | 0.6111 | 0.6712 | 0.4642 |
Cytoplasmic Foci | 0.005 | 0.001 | 0.0105 | 0.0028 | 0.0022 | 0.0053 | 0.017 | 0.0032 | 0.0093 | 0.0245 | 0.0151 | 0.0064 | 0.0056 | 0.0037 | 0.0022 | 0.0034 | 0.0026 | 0.0036 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0062 | 0.0019 | 0.0051 | 0.0057 | 0.0038 | 0.004 | 0.008 | 0.0037 | 0.0038 | 0.0028 | 0.0028 | 0.0047 | 0.0057 | 0.0038 | 0.0034 | 0.0025 | 0.0057 | 0.0047 |
Endosome | 0.0068 | 0.0006 | 0.0064 | 0.0073 | 0.0022 | 0.0054 | 0.0187 | 0.0016 | 0.0058 | 0.0031 | 0.0065 | 0.0082 | 0.0066 | 0.0026 | 0.0015 | 0.0014 | 0.0025 | 0.004 |
Golgi | 0.0014 | 0 | 0.0002 | 0.0002 | 0 | 0.0021 | 0.0091 | 0.0001 | 0.0025 | 0.0013 | 0.0079 | 0.0019 | 0.0023 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0011 |
Lipid Particles | 0.0014 | 0 | 0.0004 | 0.0001 | 0 | 0.0005 | 0.0081 | 0.0002 | 0.0051 | 0.0009 | 0.0126 | 0.0018 | 0.0073 | 0.0001 | 0 | 0 | 0 | 0.0006 |
Mitochondria | 0.0006 | 0.0002 | 0.0003 | 0.0009 | 0.0003 | 0.0008 | 0.0078 | 0.0004 | 0.0019 | 0.0038 | 0.0018 | 0.0007 | 0.0024 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0008 |
None | 0.0282 | 0.0133 | 0.0208 | 0.0195 | 0.0134 | 0.0171 | 0.0157 | 0.0106 | 0.0167 | 0.0084 | 0.0125 | 0.0259 | 0.0042 | 0.0022 | 0.0027 | 0.0057 | 0.0025 | 0.0036 |
Nuclear Periphery | 0.0246 | 0.0165 | 0.0223 | 0.0184 | 0.0176 | 0.0171 | 0.014 | 0.011 | 0.0111 | 0.0086 | 0.0074 | 0.0125 | 0.0292 | 0.0138 | 0.0148 | 0.0098 | 0.0144 | 0.0161 |
Nucleolus | 0.0006 | 0.0005 | 0.0007 | 0.0011 | 0.0009 | 0.0013 | 0.0008 | 0.0003 | 0.0008 | 0.0007 | 0.0001 | 0.0003 | 0.001 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0006 |
Nucleus | 0.4303 | 0.4921 | 0.4801 | 0.4361 | 0.404 | 0.56 | 0.3452 | 0.2786 | 0.397 | 0.1892 | 0.2108 | 0.3972 | 0.4019 | 0.3548 | 0.4156 | 0.3547 | 0.2882 | 0.48 |
Peroxisomes | 0.0006 | 0 | 0.0003 | 0.0002 | 0 | 0.0003 | 0.0112 | 0.0001 | 0.0093 | 0.0203 | 0.0321 | 0.0008 | 0.0047 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
Punctate Nuclear | 0.0072 | 0.0099 | 0.0055 | 0.0071 | 0.021 | 0.0102 | 0.0135 | 0.0079 | 0.0044 | 0.0096 | 0.0044 | 0.0081 | 0.0073 | 0.0075 | 0.0047 | 0.0068 | 0.0088 | 0.0108 |
Vacuole | 0.0026 | 0.0008 | 0.0033 | 0.0016 | 0.0016 | 0.0023 | 0.0021 | 0.0009 | 0.0011 | 0.0016 | 0.0013 | 0.0011 | 0.0027 | 0.0011 | 0.0007 | 0.0007 | 0.0009 | 0.0012 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.5719 | 44.7428 | 31.1889 | 37.7448 | 59.9776 | 43.2022 | 43.3545 | 45.569 | 41.2946 | 53.5018 |
Translational Efficiency | 0.8919 | 1.1348 | 0.8256 | 0.6526 | 0.581 | 0.8525 | 0.8125 | 0.5547 | 0.6594 | 0.7259 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1865 | 1426 | 933 | 1282 | 1664 | 2216 | 102 | 1053 | 3529 | 3642 | 1035 | 2335 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 781.19 | 953.55 | 1367.99 | 1324.35 | 954.90 | 1060.06 | 1439.43 | 1437.26 | 863.10 | 1018.36 | 1375.03 | 1375.27 |
Standard Deviation | 196.69 | 203.83 | 190.37 | 378.25 | 144.02 | 169.60 | 183.26 | 373.76 | 194.28 | 190.97 | 190.87 | 380.40 |
Intensity Change Log 2 | — | 0.287635 | 0.808312 | 0.761539 | — | 0.150724 | 0.592076 | 0.589900 | — | 0.213943 | 0.693403 | 0.669668 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000203 | 0.000287 | 0.001104 | 0.002493 | 0.000208 | 0.000418 | 0.001355 | 0.003741 | 0.000205 | 0.000367 | 0.001129 | 0.003056 |
Bud Neck | 0.006471 | 0.001517 | 0.002665 | 0.007696 | 0.001432 | 0.004313 | 0.003326 | 0.013560 | 0.004095 | 0.003218 | 0.002730 | 0.010341 |
Bud Site | 0.011866 | 0.002326 | 0.002331 | 0.021733 | 0.001850 | 0.004991 | 0.003763 | 0.044012 | 0.007144 | 0.003948 | 0.002472 | 0.031780 |
Cell Periphery | 0.000318 | 0.000091 | 0.000176 | 0.000374 | 0.000124 | 0.000068 | 0.000303 | 0.000324 | 0.000227 | 0.000077 | 0.000189 | 0.000352 |
Cytoplasm | 0.559496* | 0.526843* | 0.224374* | 0.358907* | 0.559717* | 0.512670* | 0.266572* | 0.290260* | 0.559600* | 0.518219* | 0.228533* | 0.327950* |
Cytoplasmic Foci | 0.045295 | 0.005147 | 0.002616 | 0.009343 | 0.021077 | 0.012263 | 0.003585 | 0.036942 | 0.033876 | 0.009477 | 0.002711 | 0.021789 |
Eisosomes | 0.000029 | 0.000010 | 0.000011 | 0.000019 | 0.000019 | 0.000013 | 0.000033 | 0.000027 | 0.000024 | 0.000011 | 0.000013 | 0.000023 |
Endoplasmic Reticulum | 0.001924 | 0.001415 | 0.001842 | 0.001557 | 0.002729 | 0.001633 | 0.005777 | 0.002430 | 0.002304 | 0.001548 | 0.002230 | 0.001950 |
Endosome | 0.001467 | 0.000820 | 0.000941 | 0.005284 | 0.001131 | 0.001690 | 0.001895 | 0.015925 | 0.001308 | 0.001349 | 0.001035 | 0.010083 |
Golgi | 0.000785 | 0.000507 | 0.001536 | 0.012092 | 0.000101 | 0.002432 | 0.000221 | 0.022673 | 0.000462 | 0.001679 | 0.001406 | 0.016864 |
Lipid Particles | 0.000933 | 0.000357 | 0.000591 | 0.000861 | 0.000753 | 0.000581 | 0.001339 | 0.001389 | 0.000848 | 0.000493 | 0.000664 | 0.001099 |
Mitochondria | 0.000989 | 0.000826 | 0.000792 | 0.003037 | 0.000251 | 0.001640 | 0.000699 | 0.009241 | 0.000641 | 0.001321 | 0.000783 | 0.005835 |
Mitotic Spindle | 0.001856 | 0.004728 | 0.002089 | 0.064241 | 0.001603 | 0.017039 | 0.008313 | 0.183578* | 0.001737 | 0.012219 | 0.002703 | 0.118058* |
None | 0.028100 | 0.018175 | 0.006521 | 0.017575 | 0.006593 | 0.008389 | 0.003847 | 0.011296 | 0.017959 | 0.012220 | 0.006257 | 0.014744 |
Nuclear Periphery | 0.000367 | 0.000427 | 0.000855 | 0.001184 | 0.000610 | 0.000763 | 0.001475 | 0.001747 | 0.000482 | 0.000631 | 0.000917 | 0.001438 |
Nuclear Periphery Foci | 0.001030 | 0.002146 | 0.001184 | 0.001254 | 0.002967 | 0.001174 | 0.004294 | 0.003731 | 0.001944 | 0.001554 | 0.001491 | 0.002371 |
Nucleolus | 0.001471 | 0.000590 | 0.000124 | 0.000296 | 0.000327 | 0.000955 | 0.000161 | 0.000967 | 0.000932 | 0.000812 | 0.000128 | 0.000599 |
Nucleus | 0.323920* | 0.418188* | 0.734357* | 0.454174* | 0.376288* | 0.408753* | 0.611067* | 0.285222* | 0.348613* | 0.412447* | 0.722207* | 0.377983* |
Peroxisomes | 0.000436 | 0.000156 | 0.000053 | 0.001520 | 0.000158 | 0.001011 | 0.000130 | 0.005735 | 0.000305 | 0.000676 | 0.000060 | 0.003421 |
Vacuole | 0.012348 | 0.014887 | 0.015757 | 0.034338 | 0.021976 | 0.018864 | 0.081695 | 0.062181 | 0.016888 | 0.017307 | 0.022255 | 0.046894 |
Vacuole Periphery | 0.000696 | 0.000557 | 0.000080 | 0.002021 | 0.000086 | 0.000341 | 0.000151 | 0.005020 | 0.000408 | 0.000426 | 0.000087 | 0.003373 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.79 | -3.44 | -6.05 | -4.91 | -0.55 | -2.13 | -7.60 | -5.73 | -4.95 | 1.30 | -3.50 | -3.96 | -8.32 | -6.96 | -0.50 |
Bud Neck | 7.91 | 3.72 | -6.14 | -11.52 | -9.01 | -5.09 | -4.98 | -10.28 | -7.00 | -5.32 | 1.46 | -0.61 | -11.50 | -12.07 | -11.41 |
Bud Site | 7.20 | 5.17 | -2.04 | -8.92 | -6.97 | -3.94 | -6.66 | -10.53 | -8.27 | -7.36 | 4.12 | 2.80 | -8.05 | -11.59 | -9.78 |
Cell Periphery | 10.15 | 2.28 | -5.16 | -11.11 | -6.77 | 7.05 | -6.76 | -10.63 | -13.64 | 0.80 | 12.37 | -2.60 | -9.30 | -16.21 | -7.13 |
Cytoplasm | 7.35 | 41.30 | 36.15 | 25.47 | -5.56 | 7.30 | 17.43 | 34.91 | 29.74 | -2.59 | 10.67 | 48.63 | 50.17 | 39.35 | -5.80 |
Cytoplasmic Foci | 20.08 | 21.88 | 20.05 | -0.43 | -4.87 | 5.74 | 12.25 | 7.83 | 2.86 | -3.98 | 18.24 | 24.66 | 20.56 | 3.25 | -6.21 |
Eisosomes | 8.16 | 6.29 | -4.96 | -12.87 | -11.12 | 4.61 | -6.28 | -9.06 | -13.12 | 2.58 | 9.41 | 2.81 | -9.85 | -18.62 | -11.02 |
Endoplasmic Reticulum | 0.30 | -3.10 | -1.29 | -2.29 | 2.62 | 3.77 | -4.94 | 2.03 | -2.45 | 5.51 | 2.72 | -3.44 | 0.48 | -2.92 | 4.35 |
Endosome | 3.18 | 1.81 | 0.34 | -1.38 | -0.94 | -1.27 | -3.63 | -2.69 | -1.89 | 0.11 | 0.52 | 1.05 | -1.74 | -1.91 | -2.13 |
Golgi | 1.33 | -0.18 | -1.41 | -1.81 | -1.00 | -2.95 | -5.85 | -3.05 | -0.59 | -2.81 | -2.20 | -0.60 | -2.94 | -1.30 | -1.58 |
Lipid Particles | 4.20 | -0.18 | -6.67 | -9.42 | -6.38 | 2.24 | -6.26 | -10.68 | -12.10 | -0.17 | 4.31 | -1.84 | -11.15 | -14.06 | -8.21 |
Mitochondria | 0.82 | -0.35 | -3.59 | -3.38 | -3.91 | -5.91 | -7.40 | -4.01 | -1.67 | -2.77 | -3.33 | -4.09 | -5.20 | -2.80 | -3.82 |
Mitotic Spindle | -2.61 | -1.28 | -8.61 | -7.44 | -7.93 | -6.39 | -1.24 | -13.25 | -10.47 | -5.80 | -6.89 | -1.92 | -15.61 | -11.98 | -13.84 |
None | 4.97 | 10.25 | 6.96 | 2.52 | -10.32 | -2.32 | 2.97 | -7.38 | -4.92 | -7.69 | 5.18 | 10.10 | 4.16 | -2.09 | -12.67 |
Nuclear Periphery | -3.33 | -13.07 | -18.49 | -18.41 | -9.72 | -6.97 | -7.18 | -22.87 | -19.57 | -1.47 | -7.68 | -15.35 | -28.74 | -25.56 | -14.13 |
Nuclear Periphery Foci | -4.97 | -2.80 | -2.99 | 3.26 | 0.49 | 6.04 | -1.65 | 4.17 | -2.42 | 2.89 | 1.41 | 0.29 | 2.07 | 0.44 | 1.32 |
Nucleolus | 3.68 | 5.35 | 4.59 | 2.19 | -3.72 | -2.05 | 2.78 | -2.91 | 0.90 | -4.63 | 0.58 | 5.82 | 3.13 | 1.57 | -5.88 |
Nucleus | -12.30 | -43.47 | -20.56 | -7.40 | 20.53 | -5.52 | -6.01 | -3.04 | 1.42 | 4.80 | -13.07 | -43.32 | -16.78 | -5.21 | 26.32 |
Peroxisomes | 4.26 | 6.82 | -0.03 | -1.64 | -2.22 | -2.58 | 0.14 | -2.63 | 0.34 | -2.65 | -1.81 | 6.93 | -2.10 | 0.15 | -3.64 |
Vacuole | -6.69 | -8.44 | -22.41 | -19.12 | -16.52 | -0.48 | -7.41 | -22.79 | -22.72 | 0.21 | -5.47 | -8.58 | -31.47 | -29.32 | -19.24 |
Vacuole Periphery | 1.13 | 4.95 | 1.49 | 0.25 | -1.85 | -2.64 | -5.43 | -2.62 | -1.61 | -2.11 | 0.54 | 4.65 | -0.71 | -1.03 | -3.07 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|