Standard name
Human Ortholog
Description DNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0.08 0.05 0.08 0.05 0 0.05 0 0 0 0.06 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.17 0.15 0.49 0.59 0.73 0.64 0.62 0.65 0.44 0.49 0.47 0.54 0.26 0.39 0.46 – – – 0.07 0.09 0.14 0.2 0.21 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.13 0.25 0 0.09 0 0.27 0.26 0.17 0.57 0.51 0.53 0.39 0.19 0 0 – – – 0 0 0.06 0.07 0.1 0.08
Nucleus 0.66 0.64 0.39 0.28 0.18 0.15 0.15 0.16 0.15 0.13 0.11 0.11 0.46 0.35 0.35 – – – 0.57 0.6 0.35 0.22 0.13 0.07
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.05 0 0.06 0.05 0 0.06
Vac/Vac Membrane 0.17 0.09 0 0 0 0 0 0 0 0 0 0 0.18 0.25 0.2 – – – 0.23 0.15 0.31 0.37 0.41 0.37
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 1 2 0 1 3
Bud 1 0 0 1 0 1 3 2 25 12 8 12 0 0 0 – – – 2 7 6 3 6 7
Bud Neck 1 0 36 13 30 24 20 26 15 13 5 31 1 0 0 – – – 0 2 4 2 8 3
Bud Site 0 1 0 1 0 3 5 1 5 8 6 5 0 0 0 – – – – – – – – –
Cell Periphery 0 1 1 1 0 0 2 1 1 0 1 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 32 37 214 168 267 284 338 325 234 262 282 275 70 138 140 – – – 17 26 88 41 97 111
Endoplasmic Reticulum 2 4 5 2 0 1 1 0 1 1 0 0 0 0 0 – – – 0 1 3 0 2 2
Endosome 0 0 1 0 0 1 0 1 0 0 0 0 4 24 5 – – – 3 4 5 3 8 10
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 2 0 0 0 0 0
Mitochondria 24 63 15 26 14 119 144 86 301 274 318 202 51 10 11 – – – 3 11 35 14 47 29
Nucleus 126 164 172 79 66 65 84 82 80 68 67 54 123 124 108 – – – 130 177 218 46 59 25
Nuclear Periphery 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 – – – 0 0 2 0 0 0
Nucleolus 1 0 0 0 0 1 2 0 0 1 3 0 2 0 1 – – – 3 2 2 3 7 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 1 0
SpindlePole 3 2 6 6 6 3 10 13 4 7 9 17 4 1 1 – – – 10 10 37 10 20 23
Vac/Vac Membrane 33 24 12 6 11 3 14 17 6 8 20 15 47 89 61 – – – 53 43 192 77 197 138
Unique Cell Count 191 255 438 285 368 442 546 499 531 533 603 513 268 356 307 231 295 626 211 476 369
Labelled Cell Count 223 297 465 304 394 505 623 554 672 655 719 611 302 386 327 231 295 626 211 476 369


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.1 4.7 4.1 4.8 3.6 3.9 4.1 3.2 3.2 3.2 3.6 4.1 4.3 4.3 – – – 4.1 3.9 4.0
Std Deviation (1e-4) 0.7 0.7 0.6 0.9 1.3 0.9 1.0 1.0 0.9 0.7 0.5 0.7 0.7 1.0 1.5 – – – 1.9 1.0 1.0
Intensity Change (Log2) – – – -0.19 0.03 -0.4 -0.26 -0.2 -0.55 -0.58 -0.58 -0.38 -0.2 -0.13 -0.12 – – – -0.2 -0.28 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1308 -0.8845 -1.1948 -1.1928 -1.2243 -1.0222 -0.4735 -0.1855 -0.2739 -0.4252 -0.2263 -0.2899 -0.3426 -0.3577 -0.4022 0.1096 0.0157 -0.4267
Actin 0.002 0.005 0.0003 0.0003 0.0004 0.0005 0.013 0.0001 0.0091 0.0159 0.0064 0.0019 0.0196 0.0072 0.0007 0.0009 0.0084 0.0008
Bud 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.0005 0.0022 0.0003 0.0003 0.0017 0.0003 0.0009 0.0007 0.0002 0.0005 0.0008 0.0003
Bud Neck 0.0074 0.0008 0.0009 0.0007 0.0032 0.003 0.0017 0.0009 0.003 0.003 0.0022 0.0078 0.0127 0.0012 0.0009 0.0066 0.003 0.003
Bud Periphery 0.0006 0.0002 0 0 0.0001 0.0001 0.0013 0.0006 0.0004 0.0003 0.0047 0.0009 0.0031 0.0011 0.0006 0.0022 0.001 0.0013
Bud Site 0.0013 0.0029 0.0004 0.0002 0.001 0.0003 0.0035 0.0093 0.0021 0.0013 0.004 0.0003 0.0094 0.0039 0.0009 0.0051 0.0022 0.0008
Cell Periphery 0.0002 0.0001 0 0 0 0 0.0002 0.0001 0.0002 0.0001 0.0003 0.0001 0.0004 0.0001 0.0001 0.0016 0.0002 0.0002
Cytoplasm 0.0744 0.0902 0.0724 0.0601 0.0891 0.0573 0.0799 0.0928 0.0514 0.0496 0.0753 0.0484 0.0528 0.0457 0.0279 0.0358 0.0562 0.045
Cytoplasmic Foci 0.0063 0.0055 0.0035 0.0014 0.0058 0.0041 0.0192 0.0017 0.005 0.012 0.0091 0.0031 0.0137 0.0024 0.0034 0.0032 0.0075 0.0031
Eisosomes 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0002 0 0.0002 0.0001 0.0002 0 0.0008 0 0.0001 0.0003 0.0002 0
Endoplasmic Reticulum 0.0075 0.0057 0.003 0.0021 0.0085 0.0027 0.0067 0.0032 0.0035 0.0018 0.0059 0.003 0.0034 0.0017 0.0009 0.0008 0.0047 0.0012
Endosome 0.0135 0.0075 0.0039 0.0023 0.0133 0.0049 0.027 0.0027 0.0099 0.0049 0.0175 0.007 0.0168 0.0025 0.0051 0.003 0.0247 0.0025
Golgi 0.0006 0.0008 0.0001 0 0.0002 0.0001 0.0034 0.0001 0.0031 0.0015 0.0053 0.0012 0.0042 0.0001 0.0014 0.0007 0.0052 0.0003
Lipid Particles 0.0023 0.003 0.0013 0.0002 0.0025 0.0013 0.0107 0.0002 0.0029 0.0014 0.035 0.0018 0.0082 0.0006 0.0014 0.0024 0.0084 0.001
Mitochondria 0.0015 0.0016 0.0006 0.0006 0.0009 0.0006 0.0037 0.0006 0.0047 0.0015 0.0288 0.0049 0.0147 0.0007 0.0039 0.0114 0.0052 0.0016
None 0.1496 0.1857 0.1129 0.0723 0.2379 0.1353 0.1248 0.0737 0.0864 0.0315 0.0836 0.0716 0.1528 0.1371 0.1336 0.1773 0.1158 0.0937
Nuclear Periphery 0.0684 0.0331 0.0498 0.0432 0.0469 0.0387 0.0627 0.0193 0.0293 0.0206 0.0321 0.0162 0.0318 0.0248 0.0268 0.0316 0.0327 0.0084
Nucleolus 0.0158 0.0145 0.0123 0.0289 0.0126 0.0165 0.0104 0.0205 0.0276 0.0132 0.0083 0.02 0.0196 0.0222 0.0366 0.0264 0.0156 0.0179
Nucleus 0.5778 0.5965 0.6633 0.7219 0.5201 0.6596 0.562 0.7505 0.6887 0.7891 0.6059 0.7502 0.5604 0.6776 0.7089 0.6026 0.6495 0.7511
Peroxisomes 0.0002 0.0004 0.0001 0.0001 0.0004 0.0002 0.0083 0.0001 0.0036 0.0068 0.0061 0.0004 0.0083 0.0001 0.0012 0.0019 0.0029 0.001
Punctate Nuclear 0.062 0.0402 0.0725 0.0643 0.0536 0.0717 0.0479 0.0167 0.0644 0.0425 0.0626 0.0578 0.0546 0.0679 0.043 0.0799 0.05 0.0657
Vacuole 0.0063 0.0047 0.0018 0.001 0.0027 0.0023 0.0095 0.0041 0.003 0.002 0.0035 0.0022 0.0085 0.002 0.0017 0.0047 0.0044 0.0011
Vacuole Periphery 0.002 0.001 0.0007 0.0005 0.0008 0.0006 0.0034 0.0005 0.0011 0.0005 0.0015 0.0007 0.0035 0.0004 0.0006 0.0012 0.0015 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.6683 14.9864 7.7507 6.2984 10.8679 4.0619 12.2829 9.9171 6.9562 11.7217
Translational Efficiency 0.7817 0.7138 0.6178 0.6993 0.7391 1.2107 0.5426 0.6507 0.6161 0.6003

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
234 1502 218 1021 1712 1652 82 119 1946 3154 300 1140

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 632.01 730.18 933.66 828.73 680.18 699.67 935.40 918.25 674.39 714.20 934.14 838.07
Standard Deviation 69.08 98.69 109.04 107.53 71.52 82.78 95.56 184.85 72.94 91.98 105.53 121.13
Intensity Change Log 2 — 0.208305 0.562950 0.390955 — 0.040758 0.459667 0.432970 — 0.123887 0.510336 0.412886

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000053 0.000178 0.000922 0.001329 0.000047 0.000379 0.000697 0.000961 0.000048 0.000284 0.000860 0.001290
Bud Neck 0.002768 0.007938 0.004676 0.016880 0.003267 0.014739 0.002900 0.007082 0.003207 0.011500 0.004191 0.015857
Bud Site 0.001123 0.002179 0.002091 0.021640 0.000519 0.007106 0.001220 0.007209 0.000592 0.004759 0.001853 0.020133
Cell Periphery 0.000216 0.000149 0.000120 0.000199 0.000112 0.000214 0.000110 0.000281 0.000125 0.000183 0.000117 0.000208
Cytoplasm 0.168420* 0.122607* 0.010857 0.121501* 0.204411* 0.188062* 0.062353 0.052960 0.200083* 0.156891* 0.024932 0.114347*
Cytoplasmic Foci 0.043141 0.023960 0.000673 0.007208 0.033736 0.062726 0.002139 0.022374 0.034867 0.044265 0.001074 0.008791
Eisosomes 0.000071 0.000038 0.000054 0.000040 0.000032 0.000068 0.000073 0.000044 0.000037 0.000054 0.000059 0.000040
Endoplasmic Reticulum 0.001059 0.003352 0.011453 0.008729 0.000879 0.002515 0.008269 0.019884 0.000901 0.002914 0.010582 0.009893
Endosome 0.001778 0.002353 0.000948 0.009383 0.001171 0.006040 0.001068 0.001911 0.001244 0.004284 0.000981 0.008603
Golgi 0.001219 0.001636 0.000076 0.004410 0.000457 0.002212 0.000065 0.003436 0.000548 0.001938 0.000073 0.004308
Lipid Particles 0.008165 0.003487 0.001049 0.001613 0.003074 0.008616 0.001751 0.001845 0.003686 0.006174 0.001241 0.001638
Mitochondria 0.005567 0.004861 0.000793 0.010300 0.002957 0.011378 0.001072 0.003524 0.003271 0.008275 0.000869 0.009592
Mitotic Spindle 0.000225 0.002042 0.000132 0.030658 0.000862 0.009819 0.001471 0.027166 0.000785 0.006115 0.000497 0.030294
None 0.008226 0.006201 0.001572 0.009587 0.005919 0.008019 0.002221 0.006566 0.006196 0.007153 0.001749 0.009272
Nuclear Periphery 0.001174 0.002003 0.001372 0.002732 0.001495 0.001798 0.001670 0.002213 0.001457 0.001896 0.001453 0.002678
Nuclear Periphery Foci 0.000430 0.000882 0.000478 0.001561 0.000296 0.000805 0.000505 0.009770 0.000312 0.000842 0.000486 0.002418
Nucleolus 0.004263 0.004419 0.001588 0.004061 0.001810 0.004299 0.002413 0.000799 0.002105 0.004356 0.001813 0.003721
Nucleus 0.742581* 0.799027* 0.945601* 0.689413* 0.732348* 0.646504* 0.881057* 0.727767* 0.733579* 0.719139* 0.927959* 0.693417*
Peroxisomes 0.002269 0.003541 0.000229 0.001186 0.001207 0.007504 0.000320 0.000301 0.001335 0.005616 0.000254 0.001093
Vacuole 0.006867 0.008033 0.015153 0.050158 0.005162 0.015416 0.028485 0.069166 0.005367 0.011900 0.018797 0.052142
Vacuole Periphery 0.000385 0.001113 0.000166 0.007413 0.000239 0.001779 0.000142 0.034739 0.000257 0.001462 0.000160 0.010265

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.28 -9.17 -5.71 -4.92 0.11 -4.12 -4.57 -7.77 -5.57 -0.22 -5.56 -9.79 -6.61 -5.01 0.32
Bud Neck -5.52 -4.66 -8.95 -5.26 -6.98 -6.97 -2.62 -5.55 0.05 -2.28 -8.40 -4.70 -9.49 -3.37 -6.80
Bud Site -2.14 -1.57 -6.83 -6.26 -5.52 -6.84 -4.20 -4.76 -1.16 -2.86 -7.75 -3.30 -7.69 -5.10 -5.88
Cell Periphery 1.82 2.03 1.34 -1.04 -1.41 -4.40 -1.81 -1.69 0.55 -0.77 -3.70 -0.97 -2.99 0.81 -1.57
Cytoplasm 3.10 10.80 4.73 3.50 -14.95 2.16 8.55 22.07 19.74 0.79 6.96 24.54 16.04 11.11 -9.91
Cytoplasmic Foci 2.97 7.26 6.60 10.46 -5.64 -8.00 15.42 5.46 10.25 -0.96 -3.67 17.69 14.89 20.26 -4.61
Eisosomes 2.91 0.68 0.62 -6.75 -0.23 -10.44 -5.58 -6.58 -3.53 -0.30 -6.78 -6.25 -9.63 -4.07 0.89
Endoplasmic Reticulum -7.35 -11.56 -19.69 -14.25 0.82 -5.51 -6.62 -7.31 -6.61 -1.00 -9.79 -13.36 -21.58 -16.90 0.80
Endosome -0.79 0.89 -3.09 -2.77 -4.57 -6.30 -2.38 -3.65 3.81 -0.99 -6.38 -0.97 -4.70 -0.47 -4.39
Golgi -0.42 1.22 -0.43 0.03 -3.20 -3.09 1.15 -0.74 2.11 -1.69 -2.87 1.62 -1.96 0.63 -3.34
Lipid Particles 1.22 1.93 1.77 4.26 -1.75 -6.56 2.03 2.22 7.47 0.41 -3.82 4.35 3.60 8.88 -0.99
Mitochondria 0.21 1.42 0.10 -0.24 -3.84 -5.68 1.10 1.55 7.12 0.04 -4.57 2.75 -1.26 2.69 -3.60
Mitotic Spindle -2.54 -0.73 -7.54 -6.91 -7.49 -5.12 -1.30 -2.70 -2.04 -1.77 -5.20 -1.14 -7.82 -6.19 -6.31
None 1.10 3.61 0.61 -0.86 -6.02 -2.19 5.77 0.13 1.45 -2.80 -1.23 7.08 -0.67 0.29 -6.48
Nuclear Periphery -2.85 -1.74 -9.37 -9.39 -10.14 -2.01 -4.34 -4.86 -4.36 -2.84 -4.05 -3.82 -12.18 -10.55 -9.90
Nuclear Periphery Foci -1.70 -0.94 -5.59 -3.96 -5.46 -2.69 -3.06 -1.67 -1.52 -1.45 -3.18 -3.66 -5.86 -4.61 -5.11
Nucleolus -0.45 1.86 0.06 0.76 -3.06 -7.45 -0.27 -1.75 0.61 -1.43 -6.02 1.48 -2.54 0.67 -3.13
Nucleus -2.68 -9.37 3.25 10.14 21.14 8.63 -2.47 0.60 -2.78 2.31 2.07 -14.07 4.81 3.40 16.04
Peroxisomes -1.68 2.73 2.18 5.05 -1.91 -8.52 4.44 4.71 9.97 0.44 -8.66 6.09 3.35 10.33 -1.70
Vacuole -1.13 -4.60 -17.12 -17.22 -12.75 -8.21 -4.68 -7.63 -6.86 -1.46 -8.43 -6.40 -19.28 -17.85 -9.12
Vacuole Periphery -1.80 1.56 -2.63 -1.73 -2.86 -3.69 -0.07 -1.02 -0.71 -1.01 -4.20 1.20 -2.89 -1.61 -2.95
Standard name
Human Ortholog
Description DNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p
Localization
Cell Percentages nucleus (61%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rrm3

Rrm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available