Standard name
Human Ortholog
Description 2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed; DOG2 has a paralog, DOG1, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.99 0.98 0.92 0.86 0.84 0.74 0.78 0.7 0.69 0.98 1.0 0.99 0.99 0.98 0.94 0.91 0.96 0.93 0.93 0.88 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.12 0 0.26 0.22 0.3 0.32 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.13 0.12 0.1 0.09 0.09 0.11 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 1 0 0 0 0 2 2 6 2 11 4 0 0 0 0 0 0 1 0 0 1 8 13
Bud Neck 0 0 3 1 0 1 0 1 2 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 2 3 1 8 6 1 3 6 5 2 0 2 3 2 1 0 0 0 0 0 0
Cytoplasm 150 194 287 339 357 384 484 436 354 447 342 365 238 442 455 184 218 197 150 191 271 264 268 276
Endoplasmic Reticulum 1 0 2 0 0 3 0 2 2 0 0 2 3 1 3 12 11 9 0 0 1 2 1 1
Endosome 0 0 1 0 0 0 0 1 0 1 1 5 0 0 0 0 0 0 0 0 1 2 3 2
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 3 0 1 0
Mitochondria 2 7 0 2 3 22 67 12 126 124 149 168 0 0 0 5 7 11 3 2 4 0 2 5
Nucleus 0 0 0 2 0 9 10 19 14 11 6 12 1 1 0 3 2 6 0 0 0 2 4 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 0 0 2 0 1 1 2 0 0 0 0 0 1 0 0 1 1 6 4
Vac/Vac Membrane 3 0 0 1 4 18 74 62 48 54 46 58 1 1 4 2 6 10 4 0 0 2 1 3
Unique Cell Count 153 198 294 342 364 416 565 518 479 570 490 531 243 443 461 186 223 209 166 200 292 284 306 319
Labelled Cell Count 156 202 297 348 368 439 645 544 553 644 563 626 245 445 464 210 246 236 166 200 292 284 306 319


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.2 5.8 5.6 6.7 5.7 5.7 5.7 4.9 5.1 5.1 5.4 5.9 7.0 7.0 5.6 6.5 6.9 6.8 7.2 7.8
Std Deviation (1e-4) 1.0 0.9 1.1 1.2 1.5 1.3 1.3 1.2 1.3 1.2 1.2 1.3 1.2 1.7 1.7 2.9 2.8 2.9 1.9 2.5 2.2
Intensity Change (Log2) – – – -0.05 0.2 -0.04 -0.03 -0.03 -0.24 -0.2 -0.19 -0.1 0.02 0.26 0.28 -0.06 0.16 0.26 0.23 0.31 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1361 2.0327 1.3957 0.8494 0.5158 1.6666 4.3504 5.257 4.5981 3.9402 4.7076 4.5641 1.2895 2.3432 2.1921 1.9379 1.9264 1.883
Actin 0.0056 0.0007 0.0048 0.0006 0.0154 0.0069 0.0669 0.0004 0.0128 0.0009 0.0059 0.0006 0.0217 0.0005 0.0057 0.0254 0.0083 0.0009
Bud 0.0051 0.008 0.0002 0.0001 0.0006 0.0001 0.0017 0 0.0005 0.0002 0.0018 0.0001 0.0006 0.0003 0.0007 0.0003 0.0015 0.0001
Bud Neck 0.0004 0.0002 0.0002 0.0002 0.0005 0.0019 0.0064 0 0.0029 0.0002 0.0005 0.001 0.0019 0.0001 0.0067 0.0005 0.0039 0.0005
Bud Periphery 0.0021 0.0026 0.0001 0.0001 0.0005 0.0001 0.0044 0 0.0005 0.0001 0.0021 0.0005 0.0008 0.0002 0.0007 0.0005 0.0023 0.0001
Bud Site 0.0031 0.0058 0.0016 0.0002 0.0007 0.0001 0.0085 0.0002 0.0066 0.0001 0.0008 0 0.0016 0.0007 0.0046 0.0005 0.0077 0.0001
Cell Periphery 0.0002 0.0004 0.0001 0.0001 0.0001 0 0.0111 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0002 0
Cytoplasm 0.6871 0.799 0.7683 0.6607 0.7092 0.8368 0.5735 0.71 0.6661 0.7176 0.5825 0.7958 0.6454 0.7966 0.7973 0.7289 0.6873 0.8548
Cytoplasmic Foci 0.0113 0.0061 0.0076 0.0089 0.0194 0.0087 0.0175 0.0014 0.007 0.0045 0.0079 0.0029 0.0346 0.0024 0.0063 0.0045 0.0285 0.0044
Eisosomes 0.0001 0 0 0.0001 0.0001 0 0.0019 0 0.0001 0 0.0001 0 0.0002 0 0 0.0005 0.0001 0
Endoplasmic Reticulum 0.0032 0.0021 0.0035 0.0035 0.0023 0.0019 0.0074 0.0013 0.0024 0.0016 0.0073 0.0013 0.004 0.0018 0.0031 0.0018 0.0065 0.0025
Endosome 0.0053 0.0049 0.0022 0.0048 0.0173 0.0037 0.027 0.0003 0.0055 0.0009 0.0217 0.0008 0.0097 0.0006 0.0021 0.0023 0.0477 0.0034
Golgi 0.0007 0.0005 0.0009 0.0003 0.0056 0.0003 0.0092 0.0001 0.0005 0.0001 0.0075 0.0001 0.0046 0.0001 0.0006 0.0022 0.0082 0.0003
Lipid Particles 0.0006 0.0001 0.0003 0.0005 0.0092 0.0001 0.0152 0 0.0009 0 0.0058 0 0.0048 0.0001 0.0004 0.0022 0.0069 0.0004
Mitochondria 0.0003 0.0017 0.0009 0.0004 0.0015 0.0002 0.0059 0 0.0003 0.0002 0.0051 0.0002 0.002 0.0001 0.0003 0.0127 0.0019 0.0003
None 0.265 0.1572 0.1987 0.3124 0.1997 0.1287 0.2203 0.2846 0.2858 0.2704 0.2999 0.1941 0.2467 0.1935 0.1614 0.2119 0.1491 0.1212
Nuclear Periphery 0.0024 0.0012 0.0013 0.0017 0.0019 0.001 0.0028 0.0003 0.0015 0.0006 0.0257 0.0004 0.0016 0.0004 0.001 0.0008 0.009 0.0069
Nucleolus 0.0001 0.0007 0.0001 0.0001 0.0002 0.0001 0.0032 0 0.0002 0 0.0004 0 0.0002 0 0.0001 0.0001 0.0016 0.0001
Nucleus 0.0031 0.0062 0.0046 0.0026 0.0024 0.0062 0.0032 0.0007 0.0022 0.0015 0.0171 0.0014 0.0015 0.0014 0.0021 0.0021 0.0054 0.0027
Peroxisomes 0.0006 0.0001 0.0022 0.0002 0.008 0.0005 0.0053 0 0.0005 0.0001 0.0018 0.0001 0.012 0 0.0048 0.0014 0.0056 0.0001
Punctate Nuclear 0.0026 0.0008 0.0008 0.0013 0.0033 0.002 0.0036 0.0001 0.0029 0.0005 0.0027 0.0003 0.005 0.0003 0.0012 0.0006 0.0128 0.0007
Vacuole 0.0009 0.0014 0.0013 0.0011 0.0018 0.0007 0.0041 0.0002 0.0007 0.0004 0.0021 0.0003 0.0007 0.0005 0.0005 0.0006 0.0043 0.0005
Vacuole Periphery 0.0002 0.0002 0.0001 0.0002 0.0005 0.0001 0.0011 0 0.0001 0.0001 0.0011 0.0001 0.0002 0 0.0001 0.0003 0.0011 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.7378 18.636 31.6085 32.6424 25.0985 40.1828 23.2171 23.6185 32.0071 26.3625
Translational Efficiency 1.123 1.6642 0.6557 0.9631 0.9848 1.2444 1.3838 1.1873 1.1995 1.0374

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
770 685 2261 1814 1046 2037 319 1622 1816 2722 2580 3436

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 632.16 807.22 995.35 911.93 672.07 823.18 931.63 957.10 655.15 819.16 987.47 933.25
Standard Deviation 93.32 96.99 136.78 132.68 84.05 96.03 150.86 156.71 90.28 96.52 140.18 146.27
Intensity Change Log 2 — 0.352672 0.654914 0.528633 — 0.292596 0.471146 0.510058 — 0.322028 0.563143 0.519091

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000116 0.000623 0.000219 0.003272 0.000114 0.000183 0.000612 0.000299 0.000115 0.000294 0.000268 0.001869
Bud Neck 0.021608 0.023889 0.002469 0.007936 0.004253 0.020261 0.001587 0.002840 0.011612 0.021174 0.002360 0.005530
Bud Site 0.005077 0.016244 0.009218 0.046600 0.004164 0.009599 0.003804 0.028515 0.004551 0.011271 0.008549 0.038063
Cell Periphery 0.000234 0.000240 0.000063 0.000172 0.000163 0.000092 0.000219 0.000159 0.000193 0.000129 0.000082 0.000166
Cytoplasm 0.465023* 0.448526* 0.713098* 0.569784* 0.627394* 0.563531* 0.507083* 0.709042* 0.558548* 0.534590* 0.687626* 0.635522*
Cytoplasmic Foci 0.302778* 0.268103* 0.011030 0.017984 0.127590 0.181308* 0.016479 0.015065 0.201872* 0.203150* 0.011704 0.016606
Eisosomes 0.000361 0.000156 0.000015 0.000039 0.000064 0.000090 0.000058 0.000021 0.000190 0.000107 0.000020 0.000030
Endoplasmic Reticulum 0.001010 0.000714 0.001388 0.002508 0.001000 0.000247 0.004816 0.000822 0.001004 0.000364 0.001812 0.001712
Endosome 0.010763 0.022355 0.001614 0.008694 0.006356 0.009624 0.003921 0.001875 0.008225 0.012828 0.001899 0.005475
Golgi 0.004329 0.004383 0.000070 0.003557 0.001217 0.002322 0.000274 0.002154 0.002536 0.002840 0.000096 0.002895
Lipid Particles 0.011380 0.003858 0.000604 0.000901 0.004435 0.003167 0.002120 0.000526 0.007380 0.003341 0.000792 0.000724
Mitochondria 0.007076 0.005208 0.000297 0.005037 0.001824 0.004122 0.001333 0.000383 0.004051 0.004395 0.000425 0.002840
Mitotic Spindle 0.000187 0.003265 0.005764 0.045945 0.002999 0.003653 0.001582 0.018968 0.001807 0.003556 0.005247 0.033210
None 0.005969 0.001836 0.005350 0.005148 0.010112 0.002051 0.008017 0.006475 0.008355 0.001997 0.005680 0.005774
Nuclear Periphery 0.000314 0.000274 0.001065 0.001099 0.000280 0.000222 0.000926 0.000490 0.000294 0.000236 0.001048 0.000811
Nuclear Periphery Foci 0.000334 0.000568 0.001055 0.001411 0.000857 0.000139 0.006765 0.002448 0.000635 0.000247 0.001761 0.001900
Nucleolus 0.001256 0.001044 0.000254 0.000544 0.001109 0.000774 0.000930 0.000299 0.001171 0.000842 0.000338 0.000428
Nucleus 0.119427 0.105167 0.171422* 0.157609* 0.174016* 0.130198* 0.309077* 0.126312 0.150870* 0.123899 0.188442* 0.142835*
Peroxisomes 0.005386 0.015593 0.000151 0.003207 0.001418 0.006106 0.000686 0.000294 0.003100 0.008493 0.000217 0.001832
Vacuole 0.036246 0.076604 0.074696 0.116787 0.029544 0.061392 0.127014 0.082405 0.032386 0.065220 0.081165 0.100556
Vacuole Periphery 0.001125 0.001350 0.000156 0.001768 0.001093 0.000918 0.002696 0.000609 0.001106 0.001027 0.000470 0.001221

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.60 -1.09 -2.21 -1.08 -2.03 -1.32 -9.68 -5.10 -1.52 6.99 -2.86 -2.35 -2.50 -1.75 -1.84
Bud Neck -0.61 8.56 6.83 7.08 -6.10 -9.63 1.89 1.49 12.99 -1.21 -5.90 7.92 6.13 13.78 -5.26
Bud Site -6.06 -1.47 -6.75 -1.14 -5.69 -3.93 -0.65 -5.26 -2.81 -5.49 -6.41 -1.88 -8.45 -3.87 -6.85
Cell Periphery -0.04 8.55 5.75 1.89 -6.78 7.42 -2.75 3.34 -1.05 4.74 3.38 11.27 6.71 0.94 -3.45
Cytoplasm 1.20 -13.59 -0.08 -1.43 16.80 6.67 12.18 1.74 -5.52 -11.57 3.09 -3.39 3.16 -0.06 7.32
Cytoplasmic Foci 3.13 37.41 36.90 30.17 -4.38 -8.54 24.11 24.74 38.73 -0.21 -0.10 40.70 39.99 48.29 -4.97
Eisosomes 3.79 6.53 6.12 7.48 -14.21 -4.94 -0.31 12.53 15.79 7.55 3.68 7.34 6.96 14.70 -6.81
Endoplasmic Reticulum 1.11 -1.66 -3.34 -6.70 -1.29 3.98 -8.63 -0.18 -13.79 9.02 4.26 -4.07 -3.01 -12.06 2.39
Endosome -5.49 11.44 8.00 9.69 -2.92 -3.58 3.64 7.20 15.09 4.75 -5.40 12.18 10.02 15.19 -1.74
Golgi 0.07 9.13 5.58 5.15 -2.39 -2.43 4.25 0.69 2.35 -1.31 -0.65 10.15 4.17 4.33 -2.72
Lipid Particles 7.42 11.08 10.59 8.59 -8.67 1.93 4.05 7.20 5.67 6.51 6.62 12.66 12.55 7.16 -1.41
Mitochondria 1.08 5.07 3.80 2.43 -3.27 -2.89 1.81 3.49 5.82 0.87 -0.32 5.89 4.50 5.18 -2.90
Mitotic Spindle -3.04 -7.42 -9.09 -7.28 -6.35 -0.31 1.18 -4.31 -4.53 -6.26 -1.49 -3.48 -9.23 -8.36 -7.29
None 4.08 1.04 0.84 -8.00 -0.47 5.83 3.17 3.16 -7.33 -0.56 6.99 3.59 3.20 -9.99 -1.01
Nuclear Periphery 0.62 -13.31 -16.90 -17.46 -2.78 1.68 -14.18 -13.76 -17.22 5.08 2.12 -15.25 -20.82 -22.68 0.94
Nuclear Periphery Foci -3.42 -9.89 -9.88 -7.33 -0.92 9.42 -9.38 -3.88 -6.40 7.06 7.53 -10.83 -6.78 -9.61 1.65
Nucleolus 1.29 4.91 3.96 4.25 -1.83 2.36 2.39 8.30 5.99 1.24 2.70 8.01 7.41 6.25 -0.56
Nucleus 1.85 -15.90 -13.42 -15.17 2.41 6.56 -9.90 1.01 -7.30 10.88 5.45 -14.85 -7.74 -16.13 9.20
Peroxisomes -5.32 9.56 6.79 7.91 -2.52 -6.81 4.25 6.84 8.99 1.23 -7.36 11.32 7.93 11.26 -2.42
Vacuole -9.16 -20.01 -31.79 -22.32 -15.82 -12.30 -13.66 -25.05 -18.24 3.14 -14.87 -25.60 -40.35 -30.73 -12.19
Vacuole Periphery -1.10 11.43 3.14 3.40 -2.23 1.12 -0.08 5.02 4.68 0.88 0.78 5.59 5.30 4.62 -0.91
Standard name
Human Ortholog
Description 2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed; DOG2 has a paralog, DOG1, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae
Localization
Cell Percentages cytoplasm (53%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dog2

Dog2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dog2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available