ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 1 | 8 | 9 | 5 | 0 | 0 | 2 | 3 | 5 | 0 | 6 | 6 | 11 | 9 | – | 0 | – | 1 | 2 | 8 | 1 | 6 | 8 |
Bud | 0 | 0 | 3 | 3 | 4 | 4 | 3 | 9 | 5 | 1 | 3 | 2 | 4 | 1 | 2 | – | 0 | – | 1 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 1 | 2 | 0 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 11 | 10 | 13 | 27 | 18 | 8 | 14 | 9 | 13 | 23 | 37 | 60 | 40 | – | 2 | – | 0 | 0 | 2 | 0 | 8 | 8 |
Cytoplasm | 24 | 37 | 47 | 57 | 95 | 115 | 110 | 151 | 100 | 116 | 93 | 80 | 164 | 222 | 208 | – | 9 | – | 13 | 11 | 3 | 9 | 15 | 23 |
Endoplasmic Reticulum | 122 | 80 | 143 | 140 | 70 | 83 | 111 | 56 | 87 | 112 | 49 | 106 | 259 | 229 | 207 | – | 1 | – | 148 | 111 | 211 | 165 | 211 | 186 |
Endosome | 1 | 0 | 3 | 6 | 7 | 10 | 1 | 19 | 2 | 4 | 1 | 2 | 3 | 4 | 2 | – | 0 | – | 0 | 0 | 0 | 2 | 0 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 3 | 1 | 1 | 1 | 0 | 0 |
Mitochondria | 5 | 9 | 20 | 69 | 25 | 103 | 148 | 179 | 140 | 171 | 115 | 118 | 2 | 2 | 0 | – | 4 | – | 4 | 6 | 5 | 12 | 14 | 20 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 10 | 3 | 1 | 1 | 1 | 3 | 1 | 3 | – | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 2 | 3 | 3 | 2 | 2 | 6 | 4 | 11 | 16 | 5 | 15 | 2 | 0 | 1 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 12 | 4 | 9 | 15 | 53 | 49 | 50 | 104 | 28 | 38 | 25 | 26 | 32 | 58 | 60 | – | 1 | – | 3 | 7 | 1 | 4 | 9 | 17 |
Unique Cell Count | 153 | 121 | 207 | 237 | 238 | 314 | 361 | 446 | 313 | 365 | 242 | 297 | 474 | 533 | 478 | 16 | 178 | 148 | 237 | 202 | 274 | 274 | ||
Labelled Cell Count | 172 | 135 | 247 | 312 | 275 | 395 | 448 | 543 | 393 | 477 | 305 | 379 | 512 | 588 | 534 | 19 | 178 | 148 | 237 | 202 | 274 | 274 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 6.1 | 6.4 | 6.4 | 6.8 | 5.7 | 5.2 | 5.5 | 5.4 | 5.1 | 5.3 | 5.6 | 7.5 | 7.7 | 8.0 | – | 5.6 | – | 8.1 | 8.0 | 8.2 |
Std Deviation (1e-4) | 0.8 | 0.7 | 0.9 | 1.1 | 1.8 | 1.3 | 1.0 | 1.0 | 1.1 | 1.1 | 1.0 | 1.1 | 1.1 | 0.9 | 0.9 | – | 0.9 | – | 2.1 | 1.1 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.09 | -0.16 | -0.3 | -0.21 | -0.23 | -0.31 | -0.27 | -0.19 | 0.23 | 0.28 | 0.33 | – | -0.18 | – | 0.34 | 0.33 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0 | -1.2 | 0 | -1.4 | -1.5 | – | 0 | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Cell Periphery | -0.5 | 0.1 | 1.4 | -0.2 | -2.5 | -0.4 | -1.8 | 0 | 1.1 | 1.2 | 2.5 | 1.4 | – | 0 | – |
Cytoplasm | 0.3 | 3.9 | 3.4 | 2.0 | 2.9 | 2.3 | 2.3 | 3.6 | 1.1 | 3.1 | 4.8 | 5.2 | – | 0 | – |
Endoplasmic Reticulum | -2.2 | -8.4 | -9.6 | -8.8 | -14.6 | -9.3 | -8.9 | -10.4 | -7.4 | -3.5 | -6.4 | -6.2 | – | -5.1 | – |
Endosome | 0 | 0 | 1.2 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Mitochondria | 5.1 | 0.3 | 6.1 | 7.9 | 7.9 | 8.5 | 9.1 | 8.7 | 7.4 | -6.3 | -6.7 | -6.9 | – | 0 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1.4 | 1.9 | 0 | 2.1 | 0 | 0 | 0 | – | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Vacuole | 0.9 | 5.4 | 4.0 | 3.6 | 6.0 | 2.0 | 2.5 | 2.4 | 1.9 | 1.2 | 2.8 | 3.3 | – | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7588 | 3.5736 | 3.441 | 2.9804 | 3.0431 | 3.1892 | 4.8698 | 4.9754 | 5.0331 | 3.8917 | 4.2123 | 4.5782 | 3.8213 | 4.4236 | 4.0937 | 4.1655 | 4.2788 | 4.1314 |
Actin | 0.0219 | 0.003 | 0.0264 | 0.0005 | 0.0543 | 0.0008 | 0.0056 | 0.0011 | 0.0022 | 0.0045 | 0.0072 | 0.0011 | 0.0132 | 0.0011 | 0.0108 | 0.0006 | 0.0001 | 0.0006 |
Bud | 0.0003 | 0.002 | 0.0013 | 0 | 0.0014 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Bud Neck | 0.0009 | 0.0008 | 0.001 | 0.0003 | 0.0044 | 0.0023 | 0.0027 | 0.0001 | 0.0003 | 0.0001 | 0.0089 | 0.0035 | 0.0003 | 0.0003 | 0.0014 | 0.0001 | 0.0002 | 0.0003 |
Bud Periphery | 0.0005 | 0.0007 | 0.0061 | 0.0001 | 0.0024 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0 | 0 | 0.0001 |
Bud Site | 0.0014 | 0.0068 | 0.0053 | 0.0001 | 0.0126 | 0.0001 | 0.0008 | 0.0006 | 0.0006 | 0 | 0.0003 | 0.0005 | 0.0004 | 0.0017 | 0.0026 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0015 | 0.0037 | 0.0016 | 0.0014 | 0.0018 | 0.0008 | 0.001 | 0.004 | 0.0014 | 0.0005 | 0.0004 | 0.0009 | 0.0016 | 0.0027 | 0.0033 | 0.0011 | 0.0007 | 0.0012 |
Cytoplasm | 0.175 | 0.2856 | 0.169 | 0.276 | 0.2197 | 0.2918 | 0.092 | 0.1976 | 0.1119 | 0.0949 | 0.0896 | 0.166 | 0.0644 | 0.169 | 0.1867 | 0.1177 | 0.2848 | 0.1097 |
Cytoplasmic Foci | 0.0231 | 0.0084 | 0.0202 | 0.0068 | 0.0309 | 0.0063 | 0.0151 | 0.0031 | 0.0066 | 0.0053 | 0.0111 | 0.0044 | 0.025 | 0.0027 | 0.0044 | 0.0048 | 0.0015 | 0.0042 |
Eisosomes | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.4696 | 0.55 | 0.6513 | 0.6353 | 0.4552 | 0.6172 | 0.7482 | 0.7143 | 0.8009 | 0.8591 | 0.7674 | 0.7541 | 0.7342 | 0.7616 | 0.7525 | 0.7819 | 0.6927 | 0.8347 |
Endosome | 0.0615 | 0.0143 | 0.0214 | 0.0149 | 0.0498 | 0.0155 | 0.0326 | 0.0068 | 0.0097 | 0.0145 | 0.0125 | 0.0123 | 0.0298 | 0.0052 | 0.0106 | 0.0224 | 0.0028 | 0.0057 |
Golgi | 0.0199 | 0.0013 | 0.0078 | 0.0011 | 0.0178 | 0.0008 | 0.0128 | 0.0019 | 0.0019 | 0.0041 | 0.0079 | 0.0038 | 0.0059 | 0.0009 | 0.0018 | 0.0028 | 0.0001 | 0.0006 |
Lipid Particles | 0.0269 | 0.0133 | 0.0067 | 0.0267 | 0.052 | 0.006 | 0.0132 | 0.0071 | 0.0145 | 0.0043 | 0.0194 | 0.0053 | 0.0378 | 0.0046 | 0.0064 | 0.0101 | 0.0018 | 0.006 |
Mitochondria | 0.0065 | 0.0007 | 0.0157 | 0.0005 | 0.0062 | 0.0011 | 0.0027 | 0.0002 | 0.0004 | 0.0005 | 0.0018 | 0.008 | 0.0016 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0002 |
None | 0.0655 | 0.0801 | 0.0292 | 0.02 | 0.0125 | 0.0298 | 0.0232 | 0.0459 | 0.0316 | 0.0026 | 0.0391 | 0.0193 | 0.0443 | 0.0163 | 0.0047 | 0.0452 | 0.007 | 0.024 |
Nuclear Periphery | 0.0535 | 0.0143 | 0.0106 | 0.0079 | 0.0083 | 0.0151 | 0.0269 | 0.0089 | 0.007 | 0.0061 | 0.0058 | 0.0087 | 0.0098 | 0.0108 | 0.0086 | 0.0075 | 0.0048 | 0.0061 |
Nucleolus | 0.001 | 0.0003 | 0.0005 | 0 | 0.0125 | 0.0001 | 0.0017 | 0.0001 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0265 | 0.0043 | 0.0041 | 0.0019 | 0.0029 | 0.0047 | 0.0031 | 0.0013 | 0.0011 | 0.0007 | 0.0028 | 0.0022 | 0.0016 | 0.0151 | 0.0012 | 0.0007 | 0.0007 | 0.0011 |
Peroxisomes | 0.0068 | 0.0002 | 0.0013 | 0.0001 | 0.0254 | 0.0001 | 0.0042 | 0 | 0.0052 | 0.0002 | 0.02 | 0.0003 | 0.0189 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0286 | 0.0017 | 0.0122 | 0.0003 | 0.0128 | 0.0006 | 0.005 | 0.0003 | 0.0004 | 0.0002 | 0.0015 | 0.0004 | 0.0046 | 0.0015 | 0.0007 | 0.0003 | 0.0001 | 0.0002 |
Vacuole | 0.0064 | 0.0078 | 0.0071 | 0.0055 | 0.0158 | 0.0059 | 0.0068 | 0.0063 | 0.0038 | 0.0021 | 0.0036 | 0.0078 | 0.0047 | 0.0047 | 0.0029 | 0.0037 | 0.0024 | 0.005 |
Vacuole Periphery | 0.0024 | 0.0005 | 0.0009 | 0.0004 | 0.0012 | 0.0007 | 0.0014 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.0008 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 37.698 | 26.3737 | 21.31 | 25.0037 | 26.3511 | 22.2787 | 19.8517 | 17.1981 | 22.8607 | 29.0001 |
Translational Efficiency | 0.5697 | 0.4999 | 0.687 | 0.5604 | 0.6691 | 0.8658 | 0.545 | 0.7013 | 0.575 | 0.4704 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
96 | 744 | 100 | 1473 | 1876 | 1314 | 2809 | 940 | 1972 | 2058 | 2909 | 2413 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 989.32 | 869.46 | 1188.16 | 997.14 | 845.44 | 865.00 | 1058.95 | 947.62 | 852.44 | 866.61 | 1063.39 | 977.85 |
Standard Deviation | 132.78 | 139.97 | 148.95 | 133.01 | 109.92 | 109.80 | 124.31 | 141.88 | 115.37 | 121.59 | 127.43 | 138.65 |
Intensity Change Log 2 | — | -0.186318 | 0.264220 | 0.011359 | — | 0.032998 | 0.324860 | 0.164606 | — | -0.081105 | 0.292479 | 0.084001 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000536 | 0.000645 | 0.001861 | 0.008410 | 0.000232 | 0.000808 | 0.000639 | 0.003898 | 0.000247 | 0.000749 | 0.000681 | 0.006652 |
Bud Neck | 0.003785 | 0.002416 | 0.002900 | 0.012628 | 0.001441 | 0.001708 | 0.004359 | 0.012555 | 0.001556 | 0.001964 | 0.004309 | 0.012600 |
Bud Site | 0.022074 | 0.007119 | 0.007912 | 0.044136 | 0.002337 | 0.002374 | 0.006843 | 0.034160 | 0.003298 | 0.004090 | 0.006880 | 0.040250 |
Cell Periphery | 0.000826 | 0.001267 | 0.001142 | 0.002670 | 0.000908 | 0.000986 | 0.001329 | 0.001500 | 0.000904 | 0.001088 | 0.001323 | 0.002214 |
Cytoplasm | 0.215012* | 0.164079* | 0.098607 | 0.248213* | 0.196765* | 0.201718* | 0.232551* | 0.311449* | 0.197654* | 0.188111* | 0.227947* | 0.272847* |
Cytoplasmic Foci | 0.090795 | 0.069078 | 0.021402 | 0.043099 | 0.030227 | 0.047314 | 0.007033 | 0.073026 | 0.033175 | 0.055182 | 0.007527 | 0.054757 |
Eisosomes | 0.000399 | 0.000342 | 0.000145 | 0.001327 | 0.000396 | 0.000511 | 0.000297 | 0.000669 | 0.000396 | 0.000449 | 0.000292 | 0.001071 |
Endoplasmic Reticulum | 0.047061 | 0.593841* | 0.570531* | 0.221091* | 0.658091* | 0.586954* | 0.518354* | 0.105187* | 0.628345* | 0.589443* | 0.520148* | 0.175940* |
Endosome | 0.031378 | 0.011867 | 0.018301 | 0.013303 | 0.004290 | 0.008460 | 0.004718 | 0.020085 | 0.005609 | 0.009692 | 0.005185 | 0.015945 |
Golgi | 0.013964 | 0.012142 | 0.001021 | 0.004706 | 0.009236 | 0.010335 | 0.000503 | 0.003898 | 0.009466 | 0.010988 | 0.000521 | 0.004391 |
Lipid Particles | 0.016079 | 0.043441 | 0.015828 | 0.026754 | 0.037357 | 0.052264 | 0.007842 | 0.025171 | 0.036321 | 0.049074 | 0.008117 | 0.026137 |
Mitochondria | 0.004458 | 0.004687 | 0.000197 | 0.002105 | 0.004217 | 0.007574 | 0.001011 | 0.004226 | 0.004228 | 0.006531 | 0.000983 | 0.002931 |
Mitotic Spindle | 0.001778 | 0.003165 | 0.000619 | 0.012867 | 0.000083 | 0.002158 | 0.000695 | 0.016715 | 0.000166 | 0.002522 | 0.000692 | 0.014366 |
None | 0.000753 | 0.002110 | 0.000129 | 0.000985 | 0.004921 | 0.002174 | 0.001461 | 0.002742 | 0.004718 | 0.002151 | 0.001415 | 0.001670 |
Nuclear Periphery | 0.003789 | 0.008062 | 0.025987 | 0.004014 | 0.004327 | 0.006197 | 0.017787 | 0.003668 | 0.004301 | 0.006871 | 0.018069 | 0.003879 |
Nuclear Periphery Foci | 0.029179 | 0.013460 | 0.108002 | 0.027537 | 0.005998 | 0.008142 | 0.024123 | 0.022648 | 0.007127 | 0.010065 | 0.027007 | 0.025632 |
Nucleolus | 0.000198 | 0.000669 | 0.000118 | 0.000284 | 0.000232 | 0.000279 | 0.000123 | 0.000894 | 0.000230 | 0.000420 | 0.000123 | 0.000521 |
Nucleus | 0.051308 | 0.024863 | 0.024248 | 0.107683 | 0.024822 | 0.032060 | 0.122953 | 0.085880 | 0.026112 | 0.029458 | 0.119560 | 0.099189 |
Peroxisomes | 0.001420 | 0.002852 | 0.000585 | 0.002951 | 0.001574 | 0.003266 | 0.000411 | 0.004010 | 0.001566 | 0.003116 | 0.000417 | 0.003364 |
Vacuole | 0.437355* | 0.030749 | 0.099946 | 0.212735* | 0.011523 | 0.022517 | 0.046716 | 0.263216* | 0.032253 | 0.025493 | 0.048546 | 0.232400* |
Vacuole Periphery | 0.027854 | 0.003146 | 0.000521 | 0.002502 | 0.001023 | 0.002202 | 0.000251 | 0.004407 | 0.002329 | 0.002543 | 0.000260 | 0.003244 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.01 | -2.15 | -7.21 | -7.08 | -4.96 | -1.51 | -4.77 | -3.52 | -2.65 | -2.95 | -2.04 | -5.10 | -8.23 | -7.17 | -7.47 |
Bud Neck | 2.09 | -0.56 | -9.22 | -11.46 | -3.03 | -0.75 | -6.74 | -8.84 | -8.67 | -5.90 | -1.24 | -6.69 | -15.23 | -14.97 | -9.92 |
Bud Site | 1.49 | 1.00 | -2.80 | -10.19 | -6.50 | -0.05 | -4.62 | -8.91 | -8.98 | -7.25 | -0.97 | -3.81 | -14.75 | -14.46 | -12.71 |
Cell Periphery | -1.16 | -1.68 | -8.30 | -2.32 | -4.12 | -0.48 | -3.59 | -2.05 | -1.42 | 0.46 | -0.91 | -3.76 | -7.51 | -4.00 | -4.49 |
Cytoplasm | 1.61 | 3.30 | -1.60 | -8.16 | -7.13 | -0.64 | -5.78 | -9.56 | -8.57 | -6.25 | 1.29 | -5.12 | -9.52 | -10.84 | -5.43 |
Cytoplasmic Foci | 1.15 | 5.77 | 4.41 | 5.35 | -3.49 | -5.51 | 12.77 | -10.84 | -5.35 | -19.33 | -7.64 | 13.93 | -8.27 | 0.99 | -25.58 |
Eisosomes | 0.18 | 2.86 | -6.79 | -7.12 | -8.79 | -1.63 | 1.79 | -2.11 | -0.60 | -3.11 | -1.04 | 1.93 | -6.93 | -5.79 | -7.87 |
Endoplasmic Reticulum | -31.30* | -14.95 | -10.66 | 26.61 | 11.09 | 5.69 | 15.04 | 56.71* | 42.04* | 45.26* | 3.50 | 11.80 | 51.10 | 46.40* | 43.82* |
Endosome | 2.66 | 1.31 | 2.67 | 0.07 | 2.82 | -6.70 | -1.65 | -7.48 | -4.85 | -7.05 | -7.26 | -0.11 | -9.01 | -4.13 | -9.01 |
Golgi | -0.28 | 1.60 | 1.13 | 6.00 | -3.34 | -1.14 | 15.99 | 7.32 | 6.96 | -3.68 | -1.88 | 14.80 | 7.54 | 8.87 | -5.70 |
Lipid Particles | -7.24 | -0.55 | -3.59 | 5.69 | -3.63 | -4.87 | 17.16 | 6.38 | 9.68 | -7.91 | -5.09 | 17.04 | 6.64 | 11.34 | -13.77 |
Mitochondria | -0.50 | 1.28 | 0.64 | 2.70 | -4.30 | -2.20 | 5.06 | 0.77 | 2.42 | -1.89 | -2.07 | 5.19 | 2.36 | 3.88 | -2.63 |
Mitotic Spindle | 0.13 | 0.72 | -2.84 | -3.24 | -4.18 | -2.16 | -2.93 | -5.38 | -4.38 | -5.12 | -2.50 | -2.11 | -7.79 | -5.67 | -7.44 |
None | -2.18 | 1.09 | -0.38 | 2.12 | -2.22 | 2.98 | 4.12 | 1.90 | -0.17 | -0.86 | 3.01 | 4.11 | 3.63 | 1.28 | -0.11 |
Nuclear Periphery | -3.05 | -4.77 | -0.23 | 3.20 | 4.77 | -2.37 | -12.79 | 1.45 | 3.73 | 14.02 | -3.37 | -13.42 | 1.26 | 4.68 | 14.72 |
Nuclear Periphery Foci | 1.92 | -6.54 | -0.10 | -6.57 | 7.40 | -2.00 | -15.97 | -8.01 | -6.61 | 1.61 | -3.18 | -16.40 | -13.39 | -10.74 | 2.16 |
Nucleolus | -1.81 | 1.16 | -1.84 | 1.54 | -2.63 | -0.83 | 2.38 | -2.34 | -2.13 | -2.84 | -1.80 | 2.44 | -2.35 | -0.27 | -3.68 |
Nucleus | 3.11 | 1.92 | -7.42 | -19.35 | -10.33 | -3.27 | -29.11 | -14.75 | -12.37 | 7.14 | -1.93 | -28.49 | -24.41 | -22.56 | 4.96 |
Peroxisomes | -3.72 | 1.14 | -2.65 | 0.70 | -3.43 | -2.71 | 5.75 | -4.02 | 0.01 | -6.22 | -3.63 | 5.81 | -4.15 | 0.67 | -7.09 |
Vacuole | 14.33 | 10.93 | 6.93 | -32.92 | -10.02 | -6.16 | -19.80 | -33.45 | -31.89 | -28.35 | 2.95 | -5.87 | -40.82 | -46.50 | -39.89 |
Vacuole Periphery | 1.84 | 2.12 | 1.96 | 1.47 | -2.50 | -3.16 | 5.92 | -2.14 | -0.97 | -2.88 | -0.98 | 3.52 | -0.70 | 0.07 | -4.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|