Standard name | |
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Human Ortholog | |
Description | Member of an oxysterol-binding protein family; this family has seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane; regulated by sterol binding |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 99 | 104 | 77 | 77 | 78 | 94 | 96 | 81 | 57 | – | 47 | 58 | 26 | 17 | 11 | 61 | 34 | 56 | 107 | 120 | 116 | 55 | 74 | 88 |
Bud | 20 | 21 | 29 | 34 | 40 | 55 | 52 | 48 | 43 | – | 23 | 34 | 13 | 6 | 22 | 9 | 6 | 6 | 3 | 2 | 0 | 1 | 1 | 5 |
Bud Neck | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 24 | 60 | 26 | 18 | 22 | 35 |
Bud Site | 0 | 1 | 1 | 0 | 5 | 3 | 7 | 6 | 3 | – | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 129 | 182 | 73 | 125 | 89 | 105 | 119 | 80 | 89 | – | 73 | 83 | 83 | 93 | 117 | 75 | 71 | 80 | 138 | 181 | 124 | 103 | 143 | 162 |
Cytoplasm | 77 | 126 | 134 | 110 | 116 | 148 | 173 | 223 | 115 | – | 66 | 81 | 201 | 282 | 342 | 159 | 95 | 140 | 16 | 34 | 19 | 20 | 21 | 20 |
Endoplasmic Reticulum | 25 | 37 | 12 | 7 | 40 | 25 | 61 | 34 | 41 | – | 28 | 41 | 6 | 11 | 0 | 13 | 5 | 17 | 14 | 19 | 17 | 15 | 17 | 18 |
Endosome | 0 | 0 | 2 | 0 | 8 | 12 | 3 | 8 | 1 | – | 1 | 1 | 2 | 0 | 0 | 3 | 1 | 2 | 4 | 5 | 2 | 1 | 0 | 3 |
Golgi | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 2 | 0 | 0 | 2 | 3 |
Mitochondria | 5 | 10 | 11 | 60 | 52 | 209 | 271 | 311 | 270 | – | 205 | 290 | 3 | 1 | 9 | 15 | 6 | 8 | 6 | 9 | 18 | 11 | 9 | 14 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | – | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 8 | 24 | 31 | 42 | 24 | 28 | – | 14 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4 | 3 | 8 | 4 | 9 | 6 | 8 | 5 | 9 | – | 4 | 2 | 0 | 0 | 0 | 7 | 6 | 6 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 8 | 10 | 6 | 20 | 10 | – | 3 | 2 | 0 | 0 | 0 | 3 | 2 | 1 | 4 | 13 | 5 | 4 | 4 | 4 |
Unique Cell Count | 266 | 352 | 254 | 284 | 317 | 469 | 586 | 615 | 463 | 307 | 412 | 242 | 319 | 364 | 283 | 187 | 250 | 332 | 454 | 339 | 238 | 301 | 360 | |
Labelled Cell Count | 364 | 486 | 353 | 426 | 470 | 700 | 842 | 843 | 667 | 466 | 605 | 335 | 410 | 501 | 348 | 229 | 319 | 332 | 454 | 339 | 238 | 301 | 360 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 6.8 | 6.6 | 5.7 | 5.8 | 5.2 | 4.9 | 4.9 | 5.3 | – | 4.6 | 4.3 | 6.4 | 6.3 | 5.7 | 7.3 | 8.3 | 8.2 | 6.8 | 6.8 | 7.5 |
Std Deviation (1e-4) | 1.0 | 0.8 | 3.2 | 1.2 | 2.0 | 1.2 | 0.9 | 1.3 | 4.8 | – | 2.2 | 0.9 | 0.9 | 0.9 | 0.8 | 1.1 | 1.4 | 1.4 | 1.4 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.18 | -0.33 | -0.42 | -0.41 | -0.32 | – | -0.51 | -0.61 | -0.04 | -0.07 | -0.2 | 0.16 | 0.33 | 0.31 | 0.05 | 0.05 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.8 | -1.5 | -3.1 | -4.6 | -6.0 | -5.9 | – | 0 | 0 | -5.4 | -8.0 | -9.6 | -2.3 | -2.9 | -2.0 |
Bud | 0.2 | 0.4 | 0.1 | -1.1 | -1.7 | -0.9 | – | 0 | 0 | -2.4 | -4.7 | -2.4 | -3.7 | -3.2 | -4.0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.7 | -0.2 | -1.9 | -2.7 | -5.5 | -2.9 | – | 0 | 0 | 1.3 | 0.1 | 0.9 | -0.6 | 2.0 | 0.8 |
Cytoplasm | -3.3 | -3.9 | -5.6 | -6.4 | -4.5 | -7.5 | – | -7.7 | -8.9 | 7.2 | 9.5 | 12.0 | 0.8 | -0.4 | 0.7 |
Endoplasmic Reticulum | -1.4 | 3.3 | 0.4 | 2.7 | 0.5 | 2.0 | – | 0 | 0 | -1.3 | -0.8 | 0 | -0.1 | -1.1 | 1.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.7 | 4.6 | 11.2 | 11.8 | 12.8 | 14.2 | – | 0 | 0 | -2.1 | -3.3 | -1.3 | 0.5 | -0.6 | -0.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 4.5 | 4.2 | 4.4 | 3.2 | 4.0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.4 | -0.2 | 0 | 0 | 0 | -1.0 | – | 0 | 0 | 0 | 0 | 0 | -0.5 | 0 | -0.5 |
Vacuole | 0 | 0 | 0 | 0 | 2.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0503 | 4.0764 | 4.0415 | 3.6457 | 3.8361 | 4.1977 | 5.2744 | 5.6167 | 5.7762 | 5.1095 | 5.7013 | 5.6989 | 6.329 | 6.6474 | 6.2372 | 6.0348 | 6.278 | 6.1473 |
Actin | 0.1525 | 0.0946 | 0.149 | 0.0472 | 0.1046 | 0.1014 | 0.0614 | 0.0653 | 0.1191 | 0.0673 | 0.1231 | 0.0665 | 0.1119 | 0.0642 | 0.1537 | 0.1964 | 0.1056 | 0.0968 |
Bud | 0.0017 | 0.0012 | 0.0026 | 0.0046 | 0.0024 | 0.0017 | 0.0016 | 0.0089 | 0.0033 | 0.0076 | 0.0038 | 0.0024 | 0.0014 | 0.0012 | 0.0037 | 0.0042 | 0.0018 | 0.0021 |
Bud Neck | 0.0164 | 0.0176 | 0.0694 | 0.0735 | 0.05 | 0.0991 | 0.0142 | 0.0112 | 0.0349 | 0.0297 | 0.0299 | 0.0513 | 0.0141 | 0.0091 | 0.0268 | 0.012 | 0.0208 | 0.0665 |
Bud Periphery | 0.0103 | 0.0042 | 0.021 | 0.0385 | 0.0283 | 0.0149 | 0.0079 | 0.0066 | 0.0176 | 0.0492 | 0.0303 | 0.0172 | 0.0077 | 0.005 | 0.0265 | 0.0534 | 0.0131 | 0.0142 |
Bud Site | 0.0419 | 0.2145 | 0.0784 | 0.0431 | 0.0079 | 0.0068 | 0.0255 | 0.2098 | 0.0825 | 0.024 | 0.0237 | 0.0057 | 0.0494 | 0.2533 | 0.0614 | 0.0066 | 0.0069 | 0.0076 |
Cell Periphery | 0.2382 | 0.1823 | 0.1974 | 0.1844 | 0.2169 | 0.2543 | 0.2227 | 0.1225 | 0.1276 | 0.149 | 0.1373 | 0.1731 | 0.2443 | 0.1435 | 0.1698 | 0.2199 | 0.2014 | 0.2312 |
Cytoplasm | 0.3461 | 0.4391 | 0.4183 | 0.5465 | 0.4596 | 0.4508 | 0.5664 | 0.5361 | 0.5332 | 0.6115 | 0.5392 | 0.6384 | 0.4966 | 0.4707 | 0.502 | 0.4525 | 0.5542 | 0.5328 |
Cytoplasmic Foci | 0.0191 | 0.0047 | 0.0047 | 0.0049 | 0.0108 | 0.0063 | 0.0081 | 0.0039 | 0.0086 | 0.016 | 0.015 | 0.005 | 0.0047 | 0.006 | 0.0047 | 0.0048 | 0.0179 | 0.0079 |
Eisosomes | 0.0413 | 0.0097 | 0.0134 | 0.0044 | 0.0121 | 0.0194 | 0.009 | 0.0041 | 0.0075 | 0.0016 | 0.0076 | 0.006 | 0.0126 | 0.0078 | 0.0059 | 0.0078 | 0.0053 | 0.0068 |
Endoplasmic Reticulum | 0.0157 | 0.0078 | 0.0074 | 0.0186 | 0.0042 | 0.0053 | 0.0107 | 0.0029 | 0.0042 | 0.008 | 0.0093 | 0.0045 | 0.0063 | 0.0039 | 0.0085 | 0.013 | 0.0067 | 0.0052 |
Endosome | 0.0187 | 0.001 | 0.0066 | 0.0015 | 0.0256 | 0.0031 | 0.0102 | 0.0004 | 0.0061 | 0.0008 | 0.0279 | 0.0026 | 0.0032 | 0.001 | 0.002 | 0.001 | 0.0139 | 0.0009 |
Golgi | 0.0111 | 0.0055 | 0.0044 | 0.0029 | 0.039 | 0.0047 | 0.0082 | 0.0025 | 0.0054 | 0.0035 | 0.0205 | 0.0044 | 0.0049 | 0.0027 | 0.0067 | 0.006 | 0.0088 | 0.004 |
Lipid Particles | 0.0092 | 0.0021 | 0.0023 | 0.0039 | 0.0101 | 0.0028 | 0.0058 | 0.0007 | 0.0023 | 0.0013 | 0.0051 | 0.0008 | 0.0039 | 0.0011 | 0.002 | 0.0036 | 0.0067 | 0.0012 |
Mitochondria | 0.0044 | 0.0002 | 0.0005 | 0.0004 | 0.0075 | 0.0017 | 0.0033 | 0.0101 | 0.0035 | 0.0004 | 0.0059 | 0.002 | 0.0004 | 0.0001 | 0.0068 | 0.0002 | 0.0009 | 0.0006 |
None | 0.0307 | 0.0133 | 0.0204 | 0.0218 | 0.006 | 0.0202 | 0.0297 | 0.0122 | 0.0356 | 0.0173 | 0.0083 | 0.017 | 0.0328 | 0.0281 | 0.0155 | 0.016 | 0.0264 | 0.0187 |
Nuclear Periphery | 0.0108 | 0.0004 | 0.0008 | 0.0004 | 0.0072 | 0.0019 | 0.0042 | 0.0002 | 0.0004 | 0.0009 | 0.0034 | 0.0002 | 0.0007 | 0.0003 | 0.0003 | 0.0004 | 0.0007 | 0.0002 |
Nucleolus | 0.0012 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0006 | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 |
Nucleus | 0.0166 | 0.0004 | 0.0006 | 0.0005 | 0.0011 | 0.0017 | 0.0033 | 0.0006 | 0.0009 | 0.0015 | 0.002 | 0.0006 | 0.0011 | 0.0003 | 0.0004 | 0.0003 | 0.0006 | 0.0005 |
Peroxisomes | 0.0052 | 0.0002 | 0.0006 | 0.0001 | 0.0015 | 0.0009 | 0.0026 | 0.0005 | 0.0023 | 0.0001 | 0.0016 | 0.0007 | 0.0007 | 0.0003 | 0.001 | 0.0001 | 0.0034 | 0.0003 |
Punctate Nuclear | 0.0041 | 0.0002 | 0.0005 | 0.0002 | 0.002 | 0.0012 | 0.0013 | 0.0004 | 0.0003 | 0.0067 | 0.0021 | 0.0002 | 0.0007 | 0.0005 | 0.0003 | 0.0002 | 0.0027 | 0.0007 |
Vacuole | 0.004 | 0.0011 | 0.0013 | 0.0023 | 0.0018 | 0.0015 | 0.0028 | 0.0011 | 0.0039 | 0.0029 | 0.0028 | 0.0011 | 0.002 | 0.0007 | 0.0016 | 0.0015 | 0.0018 | 0.0014 |
Vacuole Periphery | 0.0008 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0003 | 0.0006 | 0 | 0.0005 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.6391 | 28.0314 | 19.3323 | 30.2265 | 29.1766 | 26.178 | 30.8434 | 29.4816 | 32.6579 | 30.4982 |
Translational Efficiency | 0.4676 | 0.3757 | 0.588 | 0.3623 | 0.326 | 0.4696 | 0.3982 | 0.3578 | 0.3734 | 0.3784 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
434 | 125 | 1980 | 1082 | 1682 | 2149 | 142 | 125 | 2116 | 2274 | 2122 | 1207 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 739.19 | 938.24 | 1013.30 | 938.19 | 733.70 | 855.40 | 1080.54 | 928.02 | 734.83 | 859.95 | 1017.80 | 937.14 |
Standard Deviation | 93.12 | 106.36 | 113.50 | 130.70 | 115.31 | 107.11 | 111.93 | 119.03 | 111.14 | 108.72 | 114.64 | 129.57 |
Intensity Change Log 2 | — | 0.344012 | 0.455044 | 0.343935 | — | 0.221409 | 0.558490 | 0.338966 | — | 0.284241 | 0.507502 | 0.341462 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.045279 | 0.111367* | 0.207791* | 0.224248* | 0.085488 | 0.093069 | 0.086599 | 0.163712* | 0.077241 | 0.094075 | 0.199681* | 0.217979* |
Bud Neck | 0.106140 | 0.097960 | 0.069821 | 0.033206 | 0.053845 | 0.088318 | 0.032737 | 0.027636 | 0.064571 | 0.088848 | 0.067339 | 0.032630 |
Bud Site | 0.046322 | 0.113371* | 0.220128* | 0.171553* | 0.072686 | 0.104448* | 0.127640* | 0.120116* | 0.067279 | 0.104939* | 0.213939* | 0.166226* |
Cell Periphery | 0.365627* | 0.250786* | 0.088425 | 0.058271 | 0.122228* | 0.032801 | 0.056886 | 0.075108 | 0.172150* | 0.044783 | 0.086314 | 0.060014 |
Cytoplasm | 0.231751* | 0.257586* | 0.237805* | 0.231831* | 0.298360* | 0.424526* | 0.243939* | 0.181569* | 0.284698* | 0.415349* | 0.238215* | 0.226625* |
Cytoplasmic Foci | 0.009275 | 0.019883 | 0.013979 | 0.048579 | 0.061506 | 0.057884 | 0.102919 | 0.103890 | 0.050793 | 0.055795 | 0.019931 | 0.054308 |
Eisosomes | 0.121479* | 0.077416 | 0.055443 | 0.047731 | 0.074073 | 0.062447 | 0.028269 | 0.040500 | 0.083796 | 0.063270 | 0.053625 | 0.046982 |
Endoplasmic Reticulum | 0.005004 | 0.004339 | 0.021596 | 0.007205 | 0.012433 | 0.004957 | 0.030447 | 0.009469 | 0.010910 | 0.004923 | 0.022188 | 0.007439 |
Endosome | 0.002379 | 0.006234 | 0.002237 | 0.011190 | 0.020065 | 0.010478 | 0.013351 | 0.027643 | 0.016438 | 0.010244 | 0.002980 | 0.012894 |
Golgi | 0.008224 | 0.014155 | 0.002969 | 0.009552 | 0.053057 | 0.031882 | 0.006809 | 0.020307 | 0.043862 | 0.030907 | 0.003226 | 0.010666 |
Lipid Particles | 0.005266 | 0.004364 | 0.005453 | 0.013260 | 0.036543 | 0.017280 | 0.050444 | 0.036628 | 0.030128 | 0.016570 | 0.008464 | 0.015680 |
Mitochondria | 0.009553 | 0.002549 | 0.000860 | 0.002718 | 0.031234 | 0.009873 | 0.000324 | 0.000837 | 0.026787 | 0.009470 | 0.000825 | 0.002523 |
Mitotic Spindle | 0.000101 | 0.000076 | 0.000266 | 0.002824 | 0.000157 | 0.000621 | 0.000405 | 0.000234 | 0.000146 | 0.000591 | 0.000275 | 0.002556 |
None | 0.000122 | 0.000027 | 0.000344 | 0.000785 | 0.002929 | 0.000323 | 0.000107 | 0.000049 | 0.002353 | 0.000307 | 0.000328 | 0.000709 |
Nuclear Periphery | 0.000266 | 0.000039 | 0.000063 | 0.000089 | 0.000331 | 0.000074 | 0.000326 | 0.000181 | 0.000317 | 0.000072 | 0.000081 | 0.000099 |
Nuclear Periphery Foci | 0.000279 | 0.000311 | 0.001167 | 0.000881 | 0.000632 | 0.000197 | 0.005153 | 0.002364 | 0.000559 | 0.000203 | 0.001434 | 0.001035 |
Nucleolus | 0.000029 | 0.000019 | 0.000044 | 0.000197 | 0.000202 | 0.000102 | 0.000106 | 0.000072 | 0.000166 | 0.000097 | 0.000048 | 0.000184 |
Nucleus | 0.003813 | 0.003465 | 0.024932 | 0.021042 | 0.003266 | 0.011753 | 0.027529 | 0.012458 | 0.003378 | 0.011297 | 0.025106 | 0.020153 |
Peroxisomes | 0.031304 | 0.010542 | 0.003570 | 0.008739 | 0.062096 | 0.023079 | 0.011310 | 0.019431 | 0.055780 | 0.022390 | 0.004088 | 0.009846 |
Vacuole | 0.006716 | 0.025105 | 0.043003 | 0.104491 | 0.006482 | 0.024818 | 0.174507 | 0.157192 | 0.006530 | 0.024834 | 0.051803 | 0.109949 |
Vacuole Periphery | 0.001071 | 0.000407 | 0.000105 | 0.001608 | 0.002387 | 0.001071 | 0.000192 | 0.000605 | 0.002117 | 0.001034 | 0.000111 | 0.001504 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.13 | -23.19 | -18.33 | -6.09 | -1.00 | -1.57 | 0.12 | -3.54 | -3.17 | -3.19 | -3.83 | -21.61 | -16.58 | -14.30 | -1.30 |
Bud Neck | 0.57 | 4.39 | 9.02 | 5.49 | 11.76 | -8.75 | 3.42 | 5.50 | 11.84 | 0.70 | -6.17 | -0.64 | 10.40 | 16.78 | 11.85 |
Bud Site | -4.72 | -26.67* | -15.29 | -3.60 | 6.51 | -7.05 | -3.91 | -2.64 | -0.70 | 0.43 | -9.10 | -28.09 | -14.30 | -8.29 | 6.77 |
Cell Periphery | 4.06 | 19.25 | 21.26 | 7.90 | 7.22 | 17.26 | 6.65 | 3.34 | -2.72 | -1.01 | 22.01 | 14.55 | 18.11 | -3.00 | 6.49 |
Cytoplasm | -1.24 | -0.59 | -0.15 | 1.22 | 0.53 | -16.81 | 3.13 | 6.12 | 12.92 | 2.34 | -18.77 | 6.97 | 6.90 | 22.53 | 1.23 |
Cytoplasmic Foci | -2.58 | -3.09 | -11.39 | -5.77 | -10.43 | 1.11 | -3.72 | -3.29 | -3.58 | -0.12 | -1.75 | 12.60 | -1.06 | 0.24 | -10.04 |
Eisosomes | 3.60 | 8.92 | 9.77 | 3.08 | 2.68 | 3.45 | 9.08 | 5.20 | 3.57 | -1.50 | 6.18 | 9.63 | 10.37 | 5.35 | 2.53 |
Endoplasmic Reticulum | 0.42 | -8.95 | -1.56 | -2.56 | 9.57 | 5.63 | -3.17 | 1.41 | -2.16 | 3.63 | 5.38 | -6.86 | 2.82 | -3.42 | 10.15 |
Endosome | -2.55 | 0.24 | -6.38 | -2.39 | -6.70 | 6.22 | 3.17 | -1.18 | -2.86 | -2.28 | 4.83 | 11.66 | 2.29 | -1.60 | -7.28 |
Golgi | -2.32 | 3.25 | -0.40 | 2.05 | -4.12 | 7.12 | 16.46 | 4.00 | 1.53 | -1.59 | 5.13 | 18.91 | 12.71 | 10.05 | -4.42 |
Lipid Particles | 0.52 | -0.24 | -4.22 | -4.40 | -5.04 | 6.56 | -1.11 | 0.05 | -2.11 | 0.92 | 5.62 | 9.08 | 5.34 | 0.63 | -3.87 |
Mitochondria | 3.39 | 4.33 | 3.22 | -0.04 | -2.11 | 11.00 | 16.84 | 16.18 | 11.85 | -1.13 | 10.55 | 16.99 | 14.40 | 7.44 | -2.15 |
Mitotic Spindle | 1.25 | -1.38 | -2.91 | -2.93 | -2.70 | -1.89 | -1.12 | -1.15 | 1.45 | 0.67 | -1.93 | -1.14 | -2.87 | -2.23 | -2.68 |
None | 3.92 | -4.37 | -1.32 | -1.52 | -0.85 | 3.71 | 4.02 | 4.10 | 7.30 | 1.61 | 3.66 | 3.62 | 2.38 | -0.87 | -0.82 |
Nuclear Periphery | 1.21 | 1.07 | 0.91 | -3.98 | -2.26 | 1.93 | 0.06 | 1.05 | -2.26 | 1.91 | 2.17 | 2.13 | 1.92 | -1.12 | -1.68 |
Nuclear Periphery Foci | -0.28 | -6.64 | -4.85 | -5.78 | 1.18 | 2.00 | -6.87 | -3.19 | -4.27 | 3.36 | 2.04 | -4.53 | -2.91 | -7.93 | 1.91 |
Nucleolus | 2.05 | -2.80 | -1.84 | -1.95 | -1.69 | 1.65 | 1.54 | 2.02 | 1.62 | 1.28 | 1.44 | 2.42 | -0.24 | -1.12 | -1.68 |
Nucleus | 0.41 | -16.48 | -12.90 | -13.53 | 1.98 | -14.69 | -8.15 | -4.60 | -0.39 | 4.23 | -14.48 | -19.99 | -15.05 | -7.85 | 2.85 |
Peroxisomes | 4.56 | 6.49 | 5.25 | 1.14 | -5.75 | 14.76 | 11.34 | 7.96 | 0.83 | -1.32 | 14.27 | 23.50 | 19.64 | 10.31 | -5.79 |
Vacuole | -4.24 | -18.65 | -18.97 | -13.11 | -12.22 | -13.99 | -10.06 | -8.01 | -7.10 | 0.53 | -14.68 | -20.94 | -20.68 | -17.21 | -11.66 |
Vacuole Periphery | 0.96 | 1.41 | -0.45 | -1.48 | -1.89 | 4.66 | 9.09 | 5.54 | 1.84 | -1.80 | 3.93 | 8.43 | 0.96 | -0.57 | -1.95 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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