Standard name | |
---|---|
Human Ortholog | |
Description | Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | – | – | – | 1 | 4 | 2 | – | – | – | 0 | 4 | 2 | 4 | 3 | 6 | 4 | 9 | 13 | 8 | 17 | 9 | 19 |
Bud | – | – | – | – | – | 5 | 11 | 4 | – | – | – | 3 | 4 | 0 | 2 | 2 | 1 | 6 | 7 | 13 | 16 | 20 | 12 | 17 |
Bud Neck | – | – | – | – | – | 0 | 2 | 1 | – | – | – | 1 | 1 | 1 | 4 | 8 | 9 | 5 | 12 | 18 | 10 | 16 | 4 | 14 |
Bud Site | – | – | – | – | – | 0 | 4 | 0 | – | – | – | 2 | 1 | 1 | 0 | 6 | 9 | 2 | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | 6 | 9 | 3 | – | – | – | 2 | 3 | 5 | 9 | 4 | 10 | 20 | 1 | 3 | 3 | 18 | 5 | 16 |
Endoplasmic Reticulum | – | – | – | – | – | 0 | 1 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 3 | 4 | 0 | 0 | 1 | 3 | 0 | 2 |
Endosome | – | – | – | – | – | 7 | 8 | 4 | – | – | – | 1 | 2 | 1 | 0 | 2 | 6 | 2 | 1 | 2 | 1 | 4 | 4 | 7 |
Golgi | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 1 | 0 | 0 | 1 | 0 | 2 |
Mitochondria | – | – | – | – | – | 11 | 25 | 11 | – | – | – | 12 | 1 | 0 | 2 | 17 | 18 | 14 | 0 | 2 | 1 | 7 | 3 | 6 |
Nucleus | – | – | – | – | – | 3 | 2 | 1 | – | – | – | 0 | 1 | 0 | 2 | 1 | 3 | 3 | 0 | 0 | 0 | 2 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | – | – | – | – | – | 14 | 23 | 16 | – | – | – | 3 | 1 | 2 | 9 | 28 | 54 | 48 | 0 | 3 | 0 | 5 | 1 | 7 |
Peroxisomes | – | – | – | – | – | 5 | 8 | 5 | – | – | – | 5 | 5 | 1 | 4 | 3 | 8 | 3 | 4 | 2 | 4 | 1 | 2 | 2 |
SpindlePole | – | – | – | – | – | 3 | 5 | 4 | – | – | – | 3 | 4 | 3 | 3 | 3 | 4 | 4 | 5 | 3 | 3 | 9 | 1 | 7 |
Vac/Vac Membrane | – | – | – | – | – | 1 | 0 | 1 | – | – | – | 1 | 0 | 4 | 2 | 3 | 4 | 4 | 1 | 1 | 0 | 2 | 1 | 3 |
Unique Cell Count | 40 | 79 | 38 | 23 | 19 | 19 | 34 | 58 | 113 | 102 | 49 | 70 | 55 | 117 | 51 | 116 | ||||||||
Labelled Cell Count | 56 | 102 | 52 | 33 | 27 | 21 | 41 | 81 | 139 | 124 | 49 | 70 | 55 | 117 | 51 | 116 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | – | – | – | 13.8 | 12.6 | 12.6 | – | – | – | 10.6 | 16.4 | 18.1 | 15.1 | 28.2 | 28.1 | 25.7 | 16.7 | 15.9 | 16.7 |
Std Deviation (1e-4) | – | – | – | – | – | 8.2 | 7.1 | 8.1 | – | – | – | 5.6 | 6.3 | 9.0 | 6.6 | 10.1 | 19.1 | 14.6 | 9.7 | 5.0 | 6.7 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 74.8224 | 74.1394 | 59.2005 | 51.9953 | 49.674 | 54.9595 | 73.74 | 65.7178 | 58.274 | 64.2699 |
Translational Efficiency | 1.7768 | 1.732 | 1.398 | 1.5258 | 1.9523 | 1.9034 | 1.6044 | 1.2857 | 1.3784 | 1.3659 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
270 | 131 | 2014 | 324 | 20 | 1095 | 1633 | 162 | 290 | 1226 | 3647 | 486 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1173.49 | 1349.75 | 1511.17 | 1829.19 | 1241.26 | 1157.13 | 1571.66 | 1909.28 | 1178.16 | 1177.71 | 1538.26 | 1855.89 |
Standard Deviation | 314.05 | 345.43 | 425.97 | 535.88 | 368.07 | 452.61 | 460.80 | 441.31 | 318.53 | 446.38 | 442.93 | 507.73 |
Intensity Change Log 2 | — | 0.201887 | 0.364860 | 0.640399 | — | -0.101254 | 0.340484 | 0.621223 | — | 0.054019 | 0.352382 | 0.630574 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.019149 | 0.026050 | 0.006874 | 0.013128 | 0.007231 | 0.024344 | 0.003933 | 0.011559 | 0.018327 | 0.024526 | 0.005558 | 0.012605 |
Bud Neck | 0.114231 | 0.142979 | 0.192016* | 0.158786 | 0.091476 | 0.114646 | 0.207688* | 0.136895 | 0.112662 | 0.117674 | 0.199033* | 0.151489 |
Bud Site | 0.467418* | 0.442499* | 0.621978* | 0.623720* | 0.415695* | 0.514804* | 0.620753* | 0.642354* | 0.463851* | 0.507079* | 0.621429* | 0.629931* |
Cell Periphery | 0.005738 | 0.008888 | 0.007797 | 0.007471 | 0.020246 | 0.008687 | 0.009959 | 0.006893 | 0.006738 | 0.008709 | 0.008765 | 0.007278 |
Cytoplasm | 0.058614 | 0.052315 | 0.041418 | 0.024636 | 0.026628 | 0.059522 | 0.036352 | 0.027561 | 0.056408 | 0.058752 | 0.039150 | 0.025611 |
Cytoplasmic Foci | 0.161119 | 0.091995 | 0.061712 | 0.072345 | 0.107309 | 0.116961 | 0.052322 | 0.082738 | 0.157408 | 0.114293 | 0.057507 | 0.075810 |
Eisosomes | 0.007158 | 0.007921 | 0.007260 | 0.005162 | 0.008237 | 0.007266 | 0.007897 | 0.003090 | 0.007232 | 0.007336 | 0.007545 | 0.004471 |
Endoplasmic Reticulum | 0.000552 | 0.000719 | 0.000273 | 0.001794 | 0.002845 | 0.001364 | 0.000289 | 0.003135 | 0.000710 | 0.001295 | 0.000280 | 0.002241 |
Endosome | 0.004705 | 0.007530 | 0.001948 | 0.007801 | 0.012078 | 0.010101 | 0.002699 | 0.006707 | 0.005214 | 0.009827 | 0.002285 | 0.007436 |
Golgi | 0.003089 | 0.004220 | 0.000936 | 0.006469 | 0.003051 | 0.004639 | 0.001575 | 0.010861 | 0.003086 | 0.004594 | 0.001222 | 0.007933 |
Lipid Particles | 0.001649 | 0.004025 | 0.000193 | 0.003048 | 0.004047 | 0.002489 | 0.000250 | 0.000690 | 0.001814 | 0.002653 | 0.000219 | 0.002262 |
Mitochondria | 0.032782 | 0.067703 | 0.016379 | 0.022355 | 0.061549 | 0.036609 | 0.016014 | 0.015136 | 0.034766 | 0.039932 | 0.016216 | 0.019948 |
Mitotic Spindle | 0.005656 | 0.006500 | 0.004128 | 0.007183 | 0.001446 | 0.008757 | 0.004132 | 0.007312 | 0.005366 | 0.008516 | 0.004130 | 0.007226 |
None | 0.002271 | 0.003519 | 0.001316 | 0.000358 | 0.008669 | 0.009223 | 0.000985 | 0.000268 | 0.002712 | 0.008613 | 0.001168 | 0.000328 |
Nuclear Periphery | 0.000086 | 0.000130 | 0.000051 | 0.000197 | 0.000078 | 0.000131 | 0.000052 | 0.000108 | 0.000085 | 0.000131 | 0.000051 | 0.000167 |
Nuclear Periphery Foci | 0.000831 | 0.002122 | 0.000271 | 0.000662 | 0.001365 | 0.000868 | 0.000309 | 0.000304 | 0.000868 | 0.001002 | 0.000288 | 0.000543 |
Nucleolus | 0.005661 | 0.002550 | 0.001815 | 0.002603 | 0.010924 | 0.004339 | 0.002308 | 0.002207 | 0.006024 | 0.004148 | 0.002035 | 0.002471 |
Nucleus | 0.007426 | 0.002693 | 0.003655 | 0.004634 | 0.005354 | 0.009245 | 0.003734 | 0.004517 | 0.007283 | 0.008545 | 0.003691 | 0.004595 |
Peroxisomes | 0.087887 | 0.114475* | 0.017611 | 0.014578 | 0.197108* | 0.046233 | 0.014735 | 0.017285 | 0.095419 | 0.053525 | 0.016323 | 0.015480 |
Vacuole | 0.013000 | 0.010136 | 0.011052 | 0.016939 | 0.012913 | 0.015687 | 0.012478 | 0.017353 | 0.012994 | 0.015094 | 0.011690 | 0.017077 |
Vacuole Periphery | 0.000979 | 0.001030 | 0.001318 | 0.006131 | 0.001750 | 0.004084 | 0.001536 | 0.003027 | 0.001033 | 0.003758 | 0.001415 | 0.005096 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.71 | 2.47 | 1.04 | 1.41 | -1.75 | -4.32 | 1.20 | -1.02 | 2.79 | -2.19 | -1.18 | 2.79 | 1.11 | 3.21 | -2.70 |
Bud Neck | -1.48 | -7.10 | -2.84 | -0.54 | 3.29 | -0.77 | -3.95 | -1.31 | -1.30 | 4.94 | -0.47 | -8.56 | -2.80 | -3.13 | 5.69 |
Bud Site | 0.69 | -7.54 | -5.87 | -5.18 | 0.16 | -1.09 | -2.31 | -2.50 | -5.30 | -0.99 | -1.97 | -7.99 | -6.96 | -7.33 | -0.42 |
Cell Periphery | -1.07 | -1.69 | -0.52 | 0.35 | 0.13 | 0.66 | 0.59 | 0.75 | 0.84 | 1.49 | -1.14 | -1.26 | -0.22 | 0.61 | 0.68 |
Cytoplasm | 0.51 | 2.08 | 3.34 | 1.74 | 2.78 | -3.62 | -1.39 | -0.45 | 4.18 | 1.23 | -0.24 | 2.20 | 3.35 | 5.64 | 2.88 |
Cytoplasmic Foci | 3.44 | 6.75 | 5.05 | 0.92 | -1.46 | -0.36 | 1.65 | 0.69 | 2.62 | -2.41 | 2.89 | 7.26 | 5.18 | 4.00 | -2.70 |
Eisosomes | -0.25 | -0.03 | 0.86 | 1.07 | 1.30 | 0.37 | 0.16 | 1.82 | 3.61 | 4.08 | -0.04 | -0.14 | 1.36 | 2.19 | 2.63 |
Endoplasmic Reticulum | -0.48 | 2.04 | -1.38 | -1.12 | -1.72 | 0.89 | 1.56 | -0.05 | -0.62 | -1.03 | -1.57 | 2.51 | -1.37 | -0.80 | -1.80 |
Endosome | -0.97 | 2.36 | -1.16 | -0.00 | -2.50 | 0.19 | 0.89 | 0.52 | 1.31 | -1.53 | -2.73 | 2.27 | -0.96 | 1.28 | -2.88 |
Golgi | -0.84 | 2.67 | -1.34 | -0.80 | -2.36 | -0.71 | 0.71 | -1.17 | -0.97 | -1.49 | -1.47 | 2.41 | -1.77 | -1.19 | -2.58 |
Lipid Particles | -1.08 | 2.78 | -0.77 | 0.47 | -1.68 | 0.53 | 1.32 | 1.16 | 2.16 | -1.21 | -0.99 | 3.04 | -0.32 | 0.37 | -1.80 |
Mitochondria | -2.07 | 2.69 | 1.36 | 2.74 | -1.01 | 0.83 | 1.52 | 1.53 | 3.01 | 0.19 | -0.71 | 3.10 | 2.12 | 3.55 | -0.80 |
Mitotic Spindle | -0.40 | 0.93 | -0.68 | -0.33 | -1.33 | -4.29 | -3.51 | -1.62 | 0.47 | -0.81 | -1.69 | 0.79 | -0.87 | 0.53 | -1.55 |
None | -0.48 | 1.26 | 2.63 | 1.23 | 2.71 | -0.07 | 1.02 | 1.12 | 4.64 | 5.20 | -3.01 | 1.83 | 2.83 | 4.74 | 4.11 |
Nuclear Periphery | -1.07 | 2.41 | -2.23 | -0.91 | -2.88 | -1.83 | 0.77 | -0.83 | 0.51 | -1.56 | -2.37 | 2.49 | -2.31 | -1.05 | -3.22 |
Nuclear Periphery Foci | -1.16 | 2.29 | 0.53 | 1.31 | -1.60 | 0.98 | 2.23 | 2.25 | 4.07 | 0.28 | -0.52 | 2.52 | 1.21 | 2.08 | -1.53 |
Nucleolus | 1.23 | 1.60 | 1.25 | 0.06 | -0.99 | 1.01 | 1.33 | 1.34 | 1.62 | 0.17 | 0.77 | 1.75 | 1.54 | 1.99 | -0.56 |
Nucleus | 2.84 | 2.14 | 0.07 | -2.13 | -1.54 | -1.07 | 0.25 | -0.20 | 0.85 | -0.56 | -0.73 | 2.20 | 0.17 | 0.81 | -1.53 |
Peroxisomes | -1.31 | 7.12 | 7.19 | 5.54 | 1.03 | 2.24 | 2.70 | 2.67 | 5.29 | -0.50 | 3.81 | 7.63 | 7.56 | 8.62 | 0.44 |
Vacuole | 0.78 | 0.47 | -1.22 | -2.07 | -1.95 | -0.57 | 0.02 | -0.60 | -0.28 | -0.77 | -0.82 | 0.33 | -1.26 | -0.71 | -1.85 |
Vacuole Periphery | -0.33 | -1.16 | -2.27 | -2.20 | -2.09 | -1.34 | 0.13 | -0.62 | 0.64 | -0.98 | -3.00 | -1.33 | -2.54 | -0.70 | -2.27 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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