Standard name
Human Ortholog
Description Protein that functions in mitochondrial copper homeostasis; mitochondrial intermembrane space protein; essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.95 0.92 0.96 0.96 0.89 0.77 0.71 0.69 0.59 0.57 0.62 0.99 0.97 0.99 0.93 0.95 0.94 0.97 0.94 0.93 0.93 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.08 0 0.26 0.34 0.37 0.57 0.48 0.56 0.59 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.09 0.06 0.05 0.05 0 0 0 0.07 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Bud 0 0 2 0 0 0 2 5 3 1 2 3 1 2 0 0 0 0 0 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 5 2 0 0 4 2 5 0 0 0 13 4 8 0 0 0 0 0 0
Cytoplasm 282 137 56 113 161 168 203 308 167 160 81 111 186 369 367 389 200 283 276 140 70 110 198 270
Endoplasmic Reticulum 0 1 1 1 0 0 2 0 0 0 0 0 0 3 0 10 6 3 0 0 0 0 0 1
Endosome 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 1 0 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0 1 4 1 1 0 0 1 1 0
Mitochondria 0 1 5 9 4 49 89 162 138 129 80 106 1 3 0 4 3 1 2 1 0 0 2 0
Nucleus 3 1 1 1 1 0 5 11 4 7 4 2 1 2 0 1 4 2 0 1 0 1 0 1
Nuclear Periphery 0 2 0 0 0 0 3 5 2 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 1 1 0 1 7 7 10 21 22 16 7 9 0 2 2 31 5 22 2 0 1 1 2 6
Unique Cell Count 284 144 61 118 168 188 262 434 242 269 142 180 187 379 369 419 210 301 284 149 76 119 211 291
Labelled Cell Count 287 145 66 126 174 229 316 515 337 318 177 238 189 384 369 451 226 320 284 149 76 119 211 291


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 7.5 4.8 5.7 6.2 5.0 4.6 4.6 4.2 4.3 4.2 4.1 6.7 7.0 6.8 12.6 12.5 12.6 6.0 6.9 7.0
Std Deviation (1e-4) 1.0 1.0 1.0 1.1 1.4 1.2 1.2 1.2 1.3 1.2 1.3 1.0 1.2 1.0 1.0 2.4 2.6 2.5 1.1 1.5 1.2
Intensity Change (Log2) – – – 0.23 0.37 0.04 -0.07 -0.08 -0.22 -0.16 -0.21 -0.25 0.46 0.53 0.5 1.38 1.37 1.38 0.31 0.52 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 1.2 -0.6 -2.5 -3.5 -3.6 -4.8 -4.8 -4.4 3.4 2.2 4.4 0.3 1.0 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.9 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3525 1.9796 1.8205 1.3739 1.6998 1.5335 0.6185 1.1767 1.1682 1.0465 0.7067 0.8105 2.2862 3.171 2.5934 2.4956 2.1836 2.5864
Actin 0.0325 0.0006 0.004 0.1053 0.0834 0.0002 0.0067 0.0028 0.0156 0.0156 0.0312 0.0034 0.0162 0.0003 0.0036 0.0006 0.0102 0.0194
Bud 0.0005 0.0003 0.0003 0.0007 0.0003 0 0.001 0.0031 0.0037 0.0044 0.0002 0.0005 0.0004 0.0001 0.0009 0.0008 0.001 0.0001
Bud Neck 0.0215 0.0001 0.0003 0.0024 0.001 0.0004 0.0002 0.0022 0.0004 0.0024 0.0005 0.0019 0.0017 0.0001 0.0017 0.0005 0.0005 0.0006
Bud Periphery 0.0006 0.0002 0.0002 0.0007 0.0003 0 0.0007 0.0017 0.0009 0.0039 0.0002 0.0007 0.0007 0.0001 0.0008 0.001 0.0011 0.0001
Bud Site 0.0036 0.0016 0.0014 0.0015 0.0011 0 0.0012 0.0149 0.0065 0.0027 0.0005 0.0002 0.003 0.0005 0.0089 0.0056 0.0018 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0003 0.0004 0 0 0.0003 0.0001 0.0002 0.0003 0.0001 0
Cytoplasm 0.5947 0.7909 0.7458 0.5815 0.5551 0.8901 0.7044 0.7549 0.7784 0.5925 0.7203 0.8314 0.6222 0.7833 0.7698 0.7532 0.3979 0.8103
Cytoplasmic Foci 0.0574 0.0111 0.0078 0.0153 0.0172 0.0055 0.025 0.0077 0.0086 0.0146 0.0313 0.0142 0.0326 0.003 0.0127 0.0098 0.0434 0.0073
Eisosomes 0.0004 0 0 0.0001 0.0001 0 0.0001 0 0.0002 0.0003 0 0.0001 0.0004 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0054 0.002 0.0057 0.0382 0.001 0.0015 0.0035 0.0024 0.0052 0.0046 0.0007 0.0026 0.0053 0.0014 0.0023 0.0041 0.0037 0.0017
Endosome 0.0127 0.007 0.0088 0.0087 0.0141 0.0012 0.0211 0.003 0.0111 0.0166 0.0051 0.005 0.015 0.0006 0.0015 0.0217 0.0342 0.0025
Golgi 0.0135 0.0013 0.0281 0.0076 0.027 0 0.006 0.0004 0.0031 0.0367 0.0143 0.0009 0.0044 0.0001 0.0008 0.001 0.0108 0.0039
Lipid Particles 0.017 0.0005 0.0006 0.0019 0.0423 0.0001 0.0155 0.0007 0.0014 0.0197 0.0083 0.0016 0.0193 0.0001 0.0013 0.001 0.0406 0.0008
Mitochondria 0.0182 0.0005 0.0053 0.002 0.0677 0.0001 0.0105 0.0011 0.0057 0.0128 0.002 0.0206 0.0031 0.0001 0.0003 0.0008 0.004 0.0014
None 0.2008 0.1802 0.1869 0.208 0.1392 0.098 0.1623 0.1913 0.1467 0.2452 0.1032 0.0897 0.2159 0.2071 0.1867 0.1671 0.3889 0.1439
Nuclear Periphery 0.0013 0.0005 0.0013 0.0092 0.0003 0.0004 0.0083 0.0013 0.002 0.0036 0.0004 0.0036 0.0055 0.0005 0.0012 0.0035 0.0051 0.0006
Nucleolus 0.0006 0 0 0.0001 0.0001 0 0.0004 0.0016 0.0003 0.0004 0 0.0003 0.0018 0 0.0001 0.0006 0.0012 0
Nucleus 0.0012 0.0013 0.0013 0.004 0.0005 0.0016 0.0151 0.0051 0.0044 0.0065 0.0014 0.0111 0.0044 0.0018 0.0021 0.0058 0.0049 0.0037
Peroxisomes 0.0138 0.0002 0.0006 0.0099 0.0474 0 0.006 0.0014 0.0011 0.0104 0.0789 0.0006 0.0165 0.0001 0.0024 0.0004 0.0297 0.002
Punctate Nuclear 0.0028 0.0003 0.0004 0.0011 0.0008 0.0005 0.009 0.002 0.0018 0.0038 0.0008 0.0094 0.0288 0.0004 0.0022 0.0022 0.0188 0.0008
Vacuole 0.0008 0.0011 0.0008 0.0012 0.0005 0.0003 0.0022 0.0022 0.0021 0.002 0.0006 0.0009 0.0019 0.0004 0.0005 0.0172 0.0013 0.0003
Vacuole Periphery 0.0004 0.0002 0.0002 0.0004 0.0005 0 0.0006 0.0002 0.0005 0.0008 0.0001 0.0008 0.0006 0 0.0001 0.0026 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.0987 2.1631 6.4205 11.4131 9.4119 7.2552 13.6695 16.9758 18.8782 16.2094
Translational Efficiency 1.7219 9.9086 3.4069 1.888 2.245 5.9949 1.8323 2.7202 2.2603 2.3494

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1651 1219 122 1284 695 154 176 1506 2346 1373 298 2790

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 721.29 850.11 1138.67 1051.69 808.63 900.21 1060.15 1028.19 747.16 855.73 1092.30 1039.01
Standard Deviation 84.74 119.34 124.71 181.83 96.42 112.66 117.35 146.68 96.95 119.66 126.45 164.21
Intensity Change Log 2 — 0.237070 0.658698 0.544058 — 0.154782 0.390717 0.346555 — 0.194162 0.523273 0.443044

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000571 0.000316 0.001427 0.002852 0.000123 0.001656 0.000704 0.001343 0.000438 0.000466 0.001000 0.002037
Bud Neck 0.011191 0.006599 0.004363 0.024865 0.002827 0.007845 0.001663 0.003716 0.008713 0.006739 0.002769 0.013449
Bud Site 0.005013 0.011243 0.024188 0.037742 0.002798 0.012342 0.004383 0.024076 0.004357 0.011366 0.012491 0.030365
Cell Periphery 0.000107 0.000078 0.000543 0.000418 0.000176 0.000180 0.000241 0.000086 0.000127 0.000090 0.000365 0.000239
Cytoplasm 0.608036* 0.643568* 0.368331* 0.402765* 0.599591* 0.528160* 0.411002* 0.619866* 0.605534* 0.630624* 0.393533* 0.519953*
Cytoplasmic Foci 0.184467* 0.130315 0.022605 0.015094 0.183245* 0.224943* 0.011070 0.017993 0.184105* 0.140928 0.015792 0.016659
Eisosomes 0.000096 0.000046 0.000124 0.000057 0.000107 0.000126 0.000076 0.000028 0.000099 0.000055 0.000096 0.000041
Endoplasmic Reticulum 0.000718 0.000699 0.012249 0.001382 0.000808 0.000465 0.007480 0.001089 0.000745 0.000673 0.009432 0.001224
Endosome 0.012561 0.012177 0.009064 0.004570 0.011028 0.030421 0.003536 0.008292 0.012107 0.014223 0.005799 0.006579
Golgi 0.001459 0.002198 0.000311 0.002879 0.001227 0.002488 0.000133 0.003174 0.001390 0.002231 0.000206 0.003038
Lipid Particles 0.004040 0.001890 0.004900 0.000687 0.005075 0.003256 0.002377 0.000734 0.004347 0.002043 0.003410 0.000713
Mitochondria 0.001640 0.004663 0.000467 0.005342 0.001975 0.005569 0.000270 0.004755 0.001739 0.004765 0.000351 0.005025
Mitotic Spindle 0.000321 0.011284 0.000671 0.053678 0.000242 0.000542 0.002438 0.044692 0.000297 0.010079 0.001714 0.048827
None 0.004925 0.004510 0.002110 0.005612 0.001876 0.000827 0.003467 0.005400 0.004022 0.004097 0.002911 0.005498
Nuclear Periphery 0.000226 0.000324 0.001960 0.001134 0.000311 0.000211 0.001417 0.000660 0.000251 0.000312 0.001639 0.000878
Nuclear Periphery Foci 0.000534 0.001403 0.011729 0.003966 0.001024 0.001340 0.006683 0.001882 0.000679 0.001396 0.008749 0.002841
Nucleolus 0.000865 0.000930 0.000588 0.176052 0.000568 0.000872 0.000175 0.000182 0.000777 0.000923 0.000344 0.081120
Nucleus 0.099079 0.084445 0.326946* 0.177709* 0.117227 0.064013 0.336558* 0.130575* 0.104455 0.082153 0.332623* 0.152267*
Peroxisomes 0.002971 0.004045 0.000560 0.007615 0.001533 0.007808 0.000201 0.003457 0.002545 0.004467 0.000348 0.005370
Vacuole 0.060462 0.078326 0.206307* 0.073246 0.067513 0.104390 0.205985* 0.124744 0.062551 0.081250 0.206117* 0.101044
Vacuole Periphery 0.000717 0.000941 0.000558 0.002335 0.000730 0.002547 0.000141 0.003255 0.000721 0.001121 0.000312 0.002832

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.59 -2.14 -1.18 -2.60 0.96 -1.12 -6.99 -2.91 0.80 1.77 -0.10 -2.29 -1.21 -1.58 1.53
Bud Neck 3.74 5.78 -1.51 -4.70 -6.33 -3.34 1.02 -0.64 3.18 -1.90 2.04 7.10 0.77 -1.34 -6.75
Bud Site -3.83 -1.36 -7.68 -3.95 -0.84 -2.82 -3.88 -5.36 0.51 -2.91 -4.76 -1.76 -8.80 -2.22 -1.77
Cell Periphery 5.25 -5.34 -7.35 -8.52 2.67 -0.14 -2.81 9.60 5.24 6.67 6.73 -6.55 -4.09 -7.20 4.85
Cytoplasm -2.94 14.40 20.78 21.40 -4.25 3.09 13.21 12.26 2.91 -6.95 -2.21 19.60 23.67 21.23 -7.88
Cytoplasmic Foci 8.61 30.32 37.32 25.92 2.61 -2.54 28.80 29.03 14.73 -0.72 7.75 43.46 46.91 29.40 1.80
Eisosomes 9.06 -3.17 9.29 -0.08 7.61 -1.45 1.58 13.47 7.71 7.92 9.65 -1.54 14.74 5.31 10.69
Endoplasmic Reticulum -0.02 -9.02 -4.62 -7.05 8.42 2.84 -9.30 -5.15 -10.69 8.60 0.39 -12.70 -5.78 -9.18 11.81
Endosome 0.42 2.07 10.21 9.74 8.11 -3.37 6.65 7.63 4.99 2.54 -1.43 6.46 11.99 10.48 7.34
Golgi -1.37 6.05 1.47 2.24 -1.71 -1.16 3.03 1.24 1.77 -1.57 -1.75 6.92 2.22 3.06 -2.35
Lipid Particles 4.90 -1.59 6.96 3.24 7.24 2.08 3.86 7.65 2.95 7.05 6.24 1.39 9.94 4.66 9.64
Mitochondria -1.83 3.22 -0.51 1.53 -2.73 -0.93 1.42 0.55 1.18 -1.66 -1.95 3.18 0.25 2.07 -3.06
Mitotic Spindle -4.83 -2.57 -9.61 -5.49 -9.48 -0.72 -1.50 -8.06 -7.88 -4.18 -4.85 -1.58 -12.51 -5.78 -8.74
None 0.56 6.02 -1.24 -1.42 -9.39 3.25 -1.34 -10.32 -15.32 -7.95 0.08 4.39 -4.58 -2.58 -11.62
Nuclear Periphery -2.42 -14.31 -19.72 -16.16 4.66 2.33 -14.47 -23.89 -22.65 2.85 -1.75 -20.46 -31.13 -23.32 5.20
Nuclear Periphery Foci -5.29 -9.83 -4.88 -2.43 7.94 -1.20 -9.00 -5.86 -1.39 7.96 -4.82 -13.28 -7.39 -2.84 10.87
Nucleolus -0.23 2.66 -10.23 -10.22 -10.31 -0.94 6.20 6.12 2.15 -0.81 -0.81 5.26 -9.90 -9.84 -10.10
Nucleus 3.50 -11.39 -17.26 -18.88 6.26 6.36 -9.52 -13.43 -17.52 4.76 5.91 -14.92 -23.73 -26.87 7.59
Peroxisomes -1.43 5.79 0.92 2.22 -4.56 -2.31 4.06 1.58 2.58 -2.05 -2.80 7.09 2.46 4.47 -4.87
Vacuole -5.84 -10.73 -23.10 -18.79 1.55 -3.58 -12.15 -25.35 -12.03 2.75 -6.41 -16.26 -35.20 -26.95 3.19
Vacuole Periphery -1.00 2.48 -0.71 0.33 -2.23 -1.43 3.34 0.18 1.42 -1.06 -1.64 5.85 -0.03 1.06 -1.76
Standard name
Human Ortholog
Description Protein that functions in mitochondrial copper homeostasis; mitochondrial intermembrane space protein; essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
Localization
Cell Percentages cytoplasm (64%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cox23

Cox23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cox23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available