Standard name
Human Ortholog
Description DNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0.1 0.06 0.05 0.07 0.06 0.05 0.07 0.08 0.08 0.09 0 0.07 0.09 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0
Cytoplasm 0.06 0.07 0.1 0.14 0.12 0.09 0.13 0.13 0.07 0.12 0.08 0.08 0.18 0.27 0.39 0.08 0 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.21 0 0 0 0.12 0 0 0 0 0 0 0 0.13 0.14 0.07 0.3 0.24 0.24 0.15 0.12 0.11 0.11 0.13 0.14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.18 0.06 0 0 0.05 0 0.06
Mitochondria 0.67 0.84 0.8 0.88 0.72 0.9 0.84 0.8 0.93 0.91 0.9 0.92 0.72 0.54 0.59 0.1 0.19 0.11 0.64 0.66 0.67 0.62 0.58 0.53
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.1 0 0.12 0.05 0.13 0 0 0 0 0 0 0 0.07 0.13 0 0.24 0.2 0.13 0.06 0.05 0.08 0.06 0.06 0.07
SpindlePole 0.08 0 0.09 0 0.12 0 0.08 0.11 0.05 0.05 0.08 0.07 0.05 0 0 0.21 0.23 0.2 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.11 0.06 0 0 0.07 0 0 0 0 0 0 0.06 0.08 0.06 0.13 0.19 0.11 0 0 0 0 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 1 0 0 0 0 0 0 0 0 0 1 0 0 1 1 3 1 5 4 4 8 10
Bud 5 7 20 16 15 27 32 31 23 31 35 41 1 15 26 6 4 4 0 0 1 0 0 2
Bud Neck 0 0 2 0 1 1 1 2 0 1 1 0 0 0 0 0 1 1 0 1 2 1 3 3
Bud Site 0 1 3 0 5 1 17 9 5 8 15 8 0 1 2 0 1 0 – – – – – –
Cell Periphery 5 2 4 2 3 0 1 1 0 3 2 3 0 1 7 16 9 7 0 0 0 0 0 0
Cytoplasm 12 12 20 34 37 34 67 78 25 49 35 36 24 59 118 15 6 16 0 0 3 4 1 2
Endoplasmic Reticulum 5 3 2 0 1 1 1 4 0 1 0 0 0 1 1 4 1 7 0 0 1 1 1 2
Endosome 40 7 7 2 39 6 9 11 0 1 1 0 17 30 20 53 36 38 32 23 25 18 28 30
Golgi 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11 16 29 13 5 2 7 5 12
Mitochondria 128 142 161 218 224 335 442 488 311 368 376 414 94 117 178 18 28 17 144 126 146 100 132 112
Nucleus 2 0 2 1 1 2 9 18 6 10 3 4 0 1 2 1 3 4 0 0 1 1 0 0
Nuclear Periphery 3 2 1 0 0 1 2 0 1 1 6 2 0 0 0 4 0 0 1 1 1 1 1 2
Nucleolus 3 1 0 1 1 1 2 10 4 5 2 5 0 0 1 2 3 2 1 0 0 0 0 0
Peroxisomes 19 7 24 13 42 10 17 19 4 5 10 3 9 28 7 43 30 21 13 10 16 9 14 14
SpindlePole 15 4 18 10 38 15 43 65 18 21 32 33 6 5 7 38 34 32 3 3 5 2 7 6
Vac/Vac Membrane 21 19 12 0 14 26 16 18 8 9 5 6 8 17 19 23 28 18 6 7 4 5 14 6
Unique Cell Count 190 169 201 249 312 373 529 609 336 406 418 448 130 217 301 178 151 157 225 191 220 163 226 213
Labelled Cell Count 263 208 277 297 421 460 659 754 405 513 523 555 160 275 388 235 201 199 225 191 220 163 226 213


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.5 4.0 4.2 4.3 3.8 3.6 3.7 3.2 3.1 3.1 2.8 4.8 4.8 4.2 6.3 6.9 7.5 5.1 5.3 5.7
Std Deviation (1e-4) 1.0 1.3 1.3 1.8 1.8 1.7 1.7 1.7 1.2 1.4 1.3 0.8 1.5 1.9 1.6 1.2 1.3 2.3 2.1 2.3 2.1
Intensity Change (Log2) – – – 0.1 0.13 -0.06 -0.13 -0.11 -0.33 -0.33 -0.37 -0.48 0.27 0.27 0.1 0.67 0.8 0.92 0.35 0.41 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.3 3.7 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1248 1.6282 1.2281 1.2566 0.7328 1.2534 2.686 2.3851 2.3532 2.4804 2.1423 2.3743 2.3268 3.2199 2.5188 2.1071 2.4648 2.8423
Actin 0.036 0.0401 0.035 0.0369 0.0037 0.0218 0.0382 0.0396 0.0383 0.0325 0.0454 0.0231 0.0293 0.0495 0.0105 0.0023 0.0245 0.0302
Bud 0.0013 0.0025 0.0015 0.0009 0.0009 0.0008 0.0011 0.0022 0.0019 0.0036 0.002 0.0006 0.001 0.0015 0.0015 0.0005 0.0014 0.0008
Bud Neck 0.0027 0.0015 0.0055 0.0017 0.0041 0.0038 0.0082 0.0015 0.0075 0.0023 0.0011 0.0045 0.0017 0.0017 0.0022 0.0013 0.0018 0.0046
Bud Periphery 0.0022 0.0061 0.0034 0.0055 0.002 0.0015 0.0023 0.004 0.0042 0.0058 0.0158 0.0012 0.0036 0.0021 0.0036 0.0012 0.0041 0.0025
Bud Site 0.0075 0.0098 0.0101 0.0013 0.0077 0.001 0.0175 0.0209 0.0137 0.0082 0.0022 0.0014 0.0046 0.0188 0.0088 0.0021 0.0022 0.0014
Cell Periphery 0.0008 0.001 0.0039 0.0033 0.0009 0.0004 0.0007 0.0016 0.001 0.0005 0.0032 0.0003 0.0014 0.0008 0.0014 0.0003 0.0004 0.0004
Cytoplasm 0.0061 0.0065 0.0064 0.0024 0.0048 0.0052 0.0151 0.0095 0.0137 0.0087 0.0138 0.0124 0.0132 0.0075 0.0147 0.0053 0.0047 0.0101
Cytoplasmic Foci 0.0703 0.0762 0.0558 0.1005 0.0471 0.0495 0.1155 0.097 0.0735 0.0625 0.065 0.066 0.0715 0.0842 0.0487 0.0786 0.0934 0.078
Eisosomes 0.0006 0.001 0.002 0.0008 0.0002 0.0004 0.0007 0.0011 0.0008 0.0003 0.0011 0.0004 0.0013 0.0005 0.001 0.0004 0.0006 0.0005
Endoplasmic Reticulum 0.0035 0.0007 0.0046 0.0014 0.0007 0.0009 0.0042 0.0006 0.0053 0.0012 0.0023 0.0016 0.0033 0.001 0.0068 0.0005 0.0006 0.0008
Endosome 0.1162 0.1421 0.0764 0.0965 0.1181 0.0694 0.1581 0.0706 0.0991 0.1503 0.0946 0.1052 0.1019 0.0568 0.0845 0.1148 0.0792 0.0809
Golgi 0.1028 0.1371 0.0794 0.0631 0.0844 0.0815 0.1041 0.1016 0.0885 0.1009 0.1184 0.1124 0.0888 0.0779 0.0845 0.085 0.1086 0.1128
Lipid Particles 0.0773 0.038 0.0398 0.032 0.0331 0.0306 0.0763 0.0335 0.037 0.0332 0.023 0.0314 0.0508 0.0575 0.0116 0.014 0.0193 0.0376
Mitochondria 0.4535 0.4406 0.546 0.5396 0.597 0.6381 0.2828 0.4272 0.4742 0.453 0.4772 0.4841 0.4579 0.4807 0.6207 0.6205 0.4585 0.4753
None 0.0068 0.0115 0.0098 0.001 0.0012 0.0066 0.0295 0.0166 0.0076 0.0024 0.0066 0.0195 0.0517 0.0346 0.0252 0.0186 0.0332 0.0118
Nuclear Periphery 0.0065 0.0004 0.0011 0.0008 0.0003 0.0003 0.0123 0.0002 0.0016 0.001 0.0003 0.0012 0.0012 0.0002 0.0004 0.0003 0.0002 0.0004
Nucleolus 0.0023 0.0003 0.0006 0.0002 0.0012 0.0003 0.001 0.0013 0.0006 0.001 0.0004 0.0003 0.0005 0.0004 0.0004 0.0005 0.0002 0.0004
Nucleus 0.0045 0.0003 0.0005 0.0003 0.001 0.0003 0.0065 0.0004 0.0006 0.0011 0.0004 0.0004 0.0004 0.0003 0.0003 0.0003 0.0002 0.0004
Peroxisomes 0.0825 0.0758 0.1093 0.1058 0.0795 0.0816 0.1104 0.1628 0.1217 0.1155 0.1222 0.1277 0.1087 0.1187 0.0667 0.0452 0.1629 0.1421
Punctate Nuclear 0.003 0.0003 0.0005 0.0006 0.0006 0.0005 0.0062 0.0004 0.0006 0.0007 0.0004 0.0007 0.0006 0.0004 0.0002 0.0003 0.0004 0.0008
Vacuole 0.0087 0.0044 0.0055 0.0026 0.0066 0.003 0.0055 0.0058 0.0061 0.0098 0.0025 0.0028 0.0041 0.0031 0.0037 0.0032 0.0023 0.0053
Vacuole Periphery 0.0047 0.0039 0.0028 0.0027 0.005 0.0028 0.0036 0.0019 0.0025 0.0052 0.0019 0.0028 0.0028 0.0018 0.0026 0.0047 0.0014 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4938 9.2473 5.337 9.5173 11.425 9.9558 6.8393 5.6094 8.4182 13.2194
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
253 1185 1110 964 1755 1744 169 108 2008 2929 1279 1072

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 643.55 718.56 937.70 819.68 651.30 723.37 975.75 900.93 650.32 721.42 942.73 827.87
Standard Deviation 61.48 96.64 114.61 95.23 112.13 95.44 89.87 100.27 107.11 95.96 112.39 98.82
Intensity Change Log 2 — 0.159056 0.543074 0.349009 — 0.151412 0.583189 0.468093 — 0.155216 0.563391 0.410135

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000469 0.001639 0.007806 0.001879 0.001097 0.001254 0.008118 0.003537 0.001018 0.001410 0.007847 0.002046
Bud Neck 0.087550 0.027818 0.010743 0.028551 0.017372 0.018547 0.014749 0.029853 0.026214 0.022298 0.011272 0.028682
Bud Site 0.023180 0.034274 0.052544 0.043173 0.012024 0.017689 0.027154 0.069567 0.013430 0.024399 0.049189 0.045832
Cell Periphery 0.000637 0.000307 0.000204 0.000223 0.000325 0.000291 0.000205 0.000291 0.000364 0.000297 0.000204 0.000230
Cytoplasm 0.075971 0.032738 0.040928 0.037024 0.024969 0.021280 0.046148 0.027654 0.031395 0.025916 0.041618 0.036080
Cytoplasmic Foci 0.268854* 0.162813 0.181017* 0.139228 0.140289 0.136606 0.177719 0.148376 0.156488 0.147209 0.180581* 0.140150
Eisosomes 0.000360 0.000145 0.000097 0.000138 0.000226 0.000154 0.000174 0.000151 0.000243 0.000151 0.000107 0.000139
Endoplasmic Reticulum 0.000474 0.000453 0.006381 0.003138 0.000865 0.000435 0.007726 0.004087 0.000816 0.000442 0.006559 0.003234
Endosome 0.117655 0.127627 0.086519 0.159219 0.103936 0.116127 0.115340 0.176963 0.105665 0.120780 0.090327 0.161006
Golgi 0.052111 0.129348* 0.106873 0.068044 0.152345* 0.165153* 0.044175 0.054785 0.139715* 0.150667* 0.098588 0.066708
Lipid Particles 0.022376 0.007561 0.005206 0.015197 0.019976 0.009525 0.033853 0.013766 0.020278 0.008730 0.008992 0.015053
Mitochondria 0.187132* 0.290693* 0.112911* 0.171935* 0.426198* 0.387419* 0.044425 0.093480 0.396076* 0.348286* 0.103861* 0.164031*
Mitotic Spindle 0.006111 0.036527 0.193450* 0.082199 0.007522 0.013052 0.138769* 0.081441 0.007344 0.022550 0.186225* 0.082122
None 0.000701 0.001126 0.000518 0.000582 0.001952 0.000552 0.000860 0.000200 0.001795 0.000784 0.000564 0.000544
Nuclear Periphery 0.000175 0.000755 0.001184 0.001043 0.000229 0.000127 0.005293 0.000439 0.000222 0.000381 0.001727 0.000982
Nuclear Periphery Foci 0.000152 0.000457 0.003205 0.002381 0.000383 0.000246 0.011090 0.001719 0.000354 0.000331 0.004247 0.002315
Nucleolus 0.001561 0.002628 0.003493 0.002517 0.002821 0.001535 0.011581 0.001465 0.002662 0.001977 0.004562 0.002411
Nucleus 0.019293 0.005922 0.021003 0.017328 0.007832 0.002991 0.058723 0.010318 0.009276 0.004177 0.025987 0.016621
Peroxisomes 0.103513* 0.095291 0.053724 0.071252 0.050462 0.072033 0.100507* 0.135180* 0.057146 0.081443 0.059906 0.077693
Vacuole 0.024506 0.029084 0.088393 0.137668 0.016977 0.022479 0.139335 0.134879 0.017925 0.025151 0.095125 0.137387
Vacuole Periphery 0.007218 0.012794 0.023802 0.017282 0.012201 0.012504 0.014056 0.011848 0.011573 0.012621 0.022514 0.016734

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.80 -7.20 -6.55 -0.73 5.71 -0.55 -2.18 -2.72 -2.61 1.33 -1.56 -6.86 -3.72 -2.72 5.80
Bud Neck 5.19 6.74 5.13 -0.27 -7.24 -0.51 0.99 -1.99 -1.74 -2.37 1.62 6.70 -0.84 -2.54 -7.57
Bud Site -2.48 -6.73 -4.07 -2.00 2.58 -3.42 -2.96 -5.43 -4.85 -3.58 -6.51 -13.35 -9.82 -6.17 1.26
Cell Periphery 1.10 1.45 1.42 1.83 -0.33 0.48 1.59 0.54 0.22 -0.75 0.94 2.34 2.10 2.20 -0.54
Cytoplasm 4.65 3.45 3.77 -1.89 0.67 1.49 -3.15 -1.07 -1.46 1.40 2.09 -3.93 -2.58 -4.42 1.17
Cytoplasmic Foci 7.17 6.37 8.94 3.86 5.62 0.82 -2.52 0.23 -0.06 1.91 1.93 -3.19 3.19 1.75 5.94
Eisosomes 4.15 5.22 4.40 0.54 -5.06 1.66 0.88 1.48 -0.04 1.17 2.39 3.48 2.67 1.13 -3.92
Endoplasmic Reticulum 0.07 -8.60 -7.34 -7.76 4.38 1.82 -5.52 -2.67 -3.01 2.11 1.80 -9.11 -6.41 -8.39 4.93
Endosome -0.84 2.95 -2.83 -3.29 -10.30 -2.39 -0.30 -2.78 -2.16 -2.45 -3.36 4.02 -7.29 -5.08 -10.39
Golgi -10.21 -7.11 -1.60 10.22 6.63 -1.95 13.46 9.28 10.39 -0.80 -1.96 7.99 13.82 17.22 5.94
Lipid Particles 3.60 4.19 1.59 -4.13 -5.70 5.08 -2.04 1.11 -0.58 2.33 6.40 5.75 2.25 -3.72 -3.23
Mitochondria -5.33 4.32 1.42 10.43 -4.92 3.29 32.53* 17.40 15.52 -2.19 4.89 31.57 22.30 19.80 -5.52
Mitotic Spindle -8.49 -23.24* -12.21 -6.73 11.04 -3.44 -6.99 -4.47 -4.12 2.16 -8.90 -24.41 -13.13 -10.18 11.07
None -1.61 0.68 -0.25 0.98 -0.73 2.54 1.46 3.27 2.54 2.09 2.02 2.52 1.90 0.26 -0.42
Nuclear Periphery -0.99 -6.30 -5.38 -0.63 0.48 2.28 -2.44 -2.47 -3.14 2.25 -0.66 -4.97 -5.54 -2.49 2.04
Nuclear Periphery Foci -2.60 -6.52 -4.89 -4.11 1.31 1.55 -3.33 -3.54 -3.69 2.56 -0.06 -6.66 -4.97 -4.94 2.54
Nucleolus -1.44 -2.55 -1.57 0.18 1.60 2.07 -2.17 1.41 -0.14 2.43 1.15 -2.44 0.23 -1.13 2.73
Nucleus 3.27 -1.01 -0.24 -5.79 1.07 4.23 -5.41 -2.01 -3.39 4.49 4.37 -7.36 -4.63 -7.34 2.91
Peroxisomes 0.67 4.74 3.13 4.05 -2.83 -4.90 -3.55 -3.68 -2.57 -1.24 -6.31 -0.41 -3.46 1.29 -2.83
Vacuole -1.07 -12.35 -16.31 -17.07 -7.66 -2.89 -9.04 -6.66 -6.44 -0.95 -4.35 -19.81 -20.34 -19.21 -7.16
Vacuole Periphery -4.00 -8.85 -5.48 -2.21 3.57 -0.33 -0.07 0.58 0.69 0.45 -1.23 -6.91 -3.07 -2.28 3.41
Standard name
Human Ortholog
Description DNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
Localization
Cell Percentages mitochondrion (80%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Msh1

Msh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available