Standard name
Human Ortholog
Description Mitochondrial inner membrane transporter; transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0 0.05 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0.08
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.11 0.17 0.1 0 0.08 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.47 0.22 0.42 0.55 0.15 0 0 0 – 0 0 0 0.47 0.65 0.68 0.73 0.61 0.65 0.32 0.24 0.3 0.27 0.18 0.29
Mitochondria 0.12 0.4 0.55 0.47 0.73 0.78 0.77 0.71 – 0.82 0.86 0.92 0.09 0.13 0.05 0.18 0.16 0.12 0.23 0.24 0.33 0.29 0.25 0.23
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.41 0.37 0.32 0.19 0.27 0.3 0.33 0.34 – 0.23 0.2 0.18 0.24 0.11 0.09 0 0.1 0.05 0.08 0.08 0 0.05 0.07 0.05
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.35 0.25 0.1 0.16 0.16 0.11 0.07 0.07 – 0.06 0.06 0 0.53 0.29 0.37 0.1 0.1 0.19 0.13 0.2 0.12 0.18 0.25 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 13 6 11 5 7 – 5 1 2 0 7 4 2 3 5 1 2 4 2 1 8
Bud 0 0 1 1 1 2 7 7 – 4 2 12 0 2 0 1 1 1 1 5 0 2 3 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 0 0 0 1 4 2 – 6 3 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 1 0 3 – 1 2 2 0 1 1 2 1 4 0 0 0 0 0 0
Cytoplasm 0 2 0 9 6 9 17 21 – 13 13 7 0 5 9 10 12 10 1 7 1 1 1 0
Endoplasmic Reticulum 0 1 1 5 2 3 4 6 – 5 1 2 1 3 4 4 5 3 0 0 1 2 0 1
Endosome 0 0 0 0 0 1 6 5 – 8 2 3 0 1 0 1 0 0 1 1 2 1 2 1
Golgi 8 13 25 132 70 16 25 32 – 25 5 5 16 93 144 69 42 64 16 24 29 20 15 29
Mitochondria 2 24 33 113 339 405 508 599 – 527 396 602 3 18 11 17 11 12 11 24 31 22 22 23
Nucleus 0 0 0 1 0 6 8 4 – 0 4 3 1 0 1 0 0 0 1 1 0 0 3 1
Nuclear Periphery 0 0 0 0 3 4 24 23 – 35 18 33 0 0 0 0 0 0 0 0 0 1 1 2
Nucleolus 7 22 19 45 124 156 216 287 – 151 91 115 8 16 19 4 7 5 3 7 4 4 6 5
Peroxisomes 0 0 0 0 1 0 1 6 – 5 1 5 0 0 0 0 0 0 0 0 1 0 1 1
SpindlePole 0 0 0 0 1 0 1 4 – 12 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 6 15 6 38 73 57 47 61 – 40 30 22 18 41 78 9 7 19 6 20 11 13 22 19
Unique Cell Count 17 60 60 242 465 518 663 844 643 462 657 34 143 213 94 69 99 51 102 96 77 90 101
Labelled Cell Count 24 77 85 358 626 672 873 1067 837 570 821 47 187 271 119 89 123 51 102 96 77 90 101


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 56.5 70.1 49.4 52.8 38.6 25.1 20.1 18.7 – 15.5 14.3 13.0 59.4 58.3 55.9 77.3 71.8 70.8 67.4 64.2 58.2
Std Deviation (1e-4) 18.4 26.4 11.6 16.3 10.3 7.4 5.7 5.7 – 4.6 4.4 3.6 15.7 16.5 18.5 24.7 29.5 26.0 27.3 24.4 21.2
Intensity Change (Log2) – – – 0.1 -0.36 -0.97 -1.3 -1.4 – -1.67 -1.79 -1.92 0.27 0.24 0.18 0.65 0.54 0.52 0.45 0.38 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 – 0 0 0 0 0 0 0 3.4 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 1.8 -5.0 0 0 0 – 0 0 0 0.5 3.1 3.7 3.9 2.2 2.8
Mitochondria -1.2 2.9 4.0 3.7 2.6 – 5.0 5.8 8.5 -4.4 -6.4 -9.3 -4.8 -4.7 -5.8
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus -2.2 -0.8 -0.2 0.1 0.4 – -1.4 -2.1 -2.7 -0.8 -3.5 -4.5 -4.7 -3.0 -4.5
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 1.1 1.2 0.2 0 0 – 0 0 0 4.6 2.9 3.9 -0.1 0 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0042 0.0219 0.0443 0.0631 0.0046 0.0065 0.0168 0.0222 0.0682 0.0025 0.0034 0.0167
Bud – – – – – – 0.0114 0.0205 0.0198 0.0198 0.0002 0.0006 0.0032 0.0064 0.022 0.002 0.0001 0.0047
Bud Neck – – – – – – 0.0118 0.0079 0.0015 0.0007 0.0001 0.0013 0.0005 0.0022 0.0039 0.0008 0.0003 0.0395
Bud Periphery – – – – – – 0.0106 0.0208 0.0163 0.0089 0.0004 0.0016 0.0026 0.004 0.027 0.003 0.0002 0.0037
Bud Site – – – – – – 0.0465 0.0606 0.0567 0.0106 0.0003 0.0009 0.0061 0.0383 0.0248 0.0055 0.0001 0.0012
Cell Periphery – – – – – – 0.0182 0.0245 0.0141 0.0044 0.0002 0.005 0.0065 0.0163 0.0142 0.0065 0.0018 0.0083
Cytoplasm – – – – – – 0.0045 0.0036 0.0124 0.0112 0 0.0001 0.0153 0.0272 0.0037 0.0002 0 0.0099
Cytoplasmic Foci – – – – – – 0.0077 0.0218 0.0042 0.0366 0.0007 0.0006 0.1568 0.0089 0.0119 0.0095 0.0005 0.0062
Eisosomes – – – – – – 0.0007 0.0016 0.0032 0.0035 0.0003 0.0009 0.0076 0.0029 0.0021 0.0004 0.0031 0.0015
Endoplasmic Reticulum – – – – – – 0.0002 0.0002 0.0014 0.0003 0 0.0001 0.0005 0.0002 0.0003 0.0001 0 0.0029
Endosome – – – – – – 0.0149 0.0228 0.011 0.0347 0.0017 0.0028 0.009 0.0041 0.0081 0.0028 0.0021 0.0374
Golgi – – – – – – 0.0073 0.0275 0.0149 0.0214 0.0042 0.0028 0.006 0.0057 0.0054 0.0015 0.0023 0.0289
Lipid Particles – – – – – – 0.0189 0.0297 0.0113 0.0066 0.001 0.0052 0.0567 0.0252 0.0195 0.0214 0.0202 0.0182
Mitochondria – – – – – – 0.4895 0.3709 0.5855 0.6018 0.982 0.8479 0.4231 0.5471 0.5593 0.6687 0.7724 0.6196
None – – – – – – 0.0005 0.0004 0.0007 0.0002 0 0.0001 0.0184 0.0006 0.0005 0.0002 0 0.0004
Nuclear Periphery – – – – – – 0.0004 0.0004 0.0061 0.001 0 0.0001 0.0027 0.0034 0.0004 0.0002 0.0001 0.0069
Nucleolus – – – – – – 0.1213 0.1148 0.043 0.0171 0.0001 0.0105 0.0565 0.1234 0.0255 0.0443 0.0018 0.0116
Nucleus – – – – – – 0.0242 0.0235 0.0111 0.0042 0 0.0022 0.0084 0.0157 0.0066 0.0033 0.0005 0.0035
Peroxisomes – – – – – – 0.0186 0.0105 0.018 0.0077 0.0017 0.0082 0.0169 0.0101 0.0081 0.0159 0.0024 0.0044
Punctate Nuclear – – – – – – 0.0004 0.0003 0.0003 0.0002 0 0 0.0067 0.0002 0.0003 0.0001 0 0.0001
Vacuole – – – – – – 0.0936 0.1083 0.0548 0.0388 0.0006 0.0363 0.0666 0.0574 0.1088 0.058 0.0276 0.0503
Vacuole Periphery – – – – – – 0.0948 0.1076 0.0693 0.107 0.0017 0.0665 0.1132 0.0787 0.0793 0.1532 0.161 0.1241

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.8047 19.2217 18.0168 19.3687 15.4066 20.3593 20.2022 17.6048 17.4782 24.4277
Translational Efficiency 1.4586 1.2851 1.0649 0.8869 1.2449 1.0119 1.1335 0.9827 1.1376 0.962

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
435 965 1986 1130 1448 1040 718 236 1883 2005 2704 1366

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4153.92 5127.21 4890.15 5217.48 5059.14 4484.82 4860.43 4227.37 4850.02 4794.00 4882.26 5046.42
Standard Deviation 1182.44 1496.93 2093.93 1864.33 1311.98 1324.72 1306.91 1524.24 1338.73 1446.30 1916.77 1848.44
Intensity Change Log 2 — 0.303701 0.235405 0.328880 — -0.173842 -0.057808 -0.259132 — 0.061161 0.081808 0.035847

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002815 0.005909 0.003909 0.003321 0.000751 0.009396 0.003815 0.010338 0.001228 0.007718 0.003884 0.004534
Bud Neck 0.006344 0.011125 0.017797 0.024903 0.003632 0.012294 0.015662 0.034973 0.004258 0.011731 0.017230 0.026643
Bud Site 0.016494 0.047523 0.110740* 0.110733* 0.015346 0.029710 0.054403 0.122792* 0.015611 0.038283 0.095781 0.112816*
Cell Periphery 0.001824 0.004535 0.008359 0.006475 0.003492 0.002228 0.003647 0.003164 0.003107 0.003338 0.007108 0.005903
Cytoplasm 0.003071 0.005432 0.039043 0.016306 0.002795 0.003820 0.007561 0.018064 0.002859 0.004596 0.030684 0.016609
Cytoplasmic Foci 0.048889 0.061559 0.053351 0.052191 0.026471 0.066322 0.054852 0.095262 0.031650 0.064030 0.053750 0.059632
Eisosomes 0.000339 0.000775 0.000281 0.000531 0.000100 0.000446 0.000309 0.000634 0.000155 0.000605 0.000288 0.000548
Endoplasmic Reticulum 0.000429 0.000622 0.003196 0.000625 0.000519 0.000570 0.001282 0.000945 0.000498 0.000595 0.002687 0.000680
Endosome 0.011704 0.014740 0.021726 0.011824 0.014047 0.019209 0.013496 0.019152 0.013505 0.017058 0.019541 0.013090
Golgi 0.090767 0.082768 0.070398 0.033594 0.105424 0.114620* 0.077799 0.045184 0.102038 0.099290 0.072363 0.035596
Lipid Particles 0.004654 0.006636 0.002838 0.001612 0.004218 0.011128 0.003248 0.007459 0.004319 0.008966 0.002947 0.002622
Mitochondria 0.271609* 0.371149* 0.251929* 0.374107* 0.224793* 0.338905* 0.305432* 0.274078* 0.235608* 0.354424* 0.266135* 0.356825*
Mitotic Spindle 0.005263 0.004038 0.013440 0.012943 0.003368 0.006407 0.009968 0.023898 0.003806 0.005267 0.012518 0.014836
None 0.004797 0.004538 0.004289 0.003777 0.002994 0.003401 0.003102 0.002971 0.003411 0.003948 0.003974 0.003638
Nuclear Periphery 0.002240 0.001332 0.001140 0.000810 0.001195 0.002213 0.002480 0.001001 0.001436 0.001789 0.001496 0.000843
Nuclear Periphery Foci 0.002078 0.001629 0.001745 0.000963 0.002070 0.002716 0.001435 0.001986 0.002071 0.002193 0.001663 0.001140
Nucleolus 0.004890 0.005577 0.004916 0.010116 0.002680 0.007214 0.011166 0.014051 0.003190 0.006427 0.006576 0.010796
Nucleus 0.001285 0.002134 0.010122 0.007370 0.000928 0.001300 0.001983 0.004026 0.001011 0.001701 0.007960 0.006793
Peroxisomes 0.003078 0.005871 0.002320 0.004621 0.000992 0.005149 0.002850 0.009981 0.001474 0.005497 0.002460 0.005547
Vacuole 0.146156 0.141146 0.168979 0.148507 0.180552 0.155613 0.158500 0.154310 0.172606 0.148650 0.166197 0.149510
Vacuole Periphery 0.371274* 0.220961* 0.209484* 0.174672* 0.403633* 0.207338* 0.267010* 0.155732* 0.396158* 0.213894* 0.224759* 0.171400*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.66 -1.40 -0.71 2.40 0.90 -5.64 -4.64 -2.25 -0.12 -1.48 -6.86 -6.18 -4.06 2.70 -0.68
Bud Neck -4.18 -9.84 -11.38 -8.45 -4.05 -8.42 -9.37 -7.93 -5.63 -4.59 -10.30 -16.14 -15.81 -9.97 -5.86
Bud Site -6.99 -20.09 -15.92 -9.86 -0.02 -4.99 -8.65 -8.35 -7.15 -4.95 -9.02 -23.71 -19.06 -13.79 -2.89
Cell Periphery -5.51 -8.16 -6.80 -2.59 1.89 2.50 -0.26 0.59 -1.31 0.79 -0.49 -5.81 -4.26 -4.48 1.55
Cytoplasm -2.09 -14.92 -8.39 -6.77 8.20 -2.04 -4.25 -3.85 -3.54 -2.75 -3.07 -15.58 -9.45 -8.05 5.73
Cytoplasmic Foci -1.76 -1.43 -1.05 0.97 0.42 -8.47 -5.63 -6.46 -2.83 -3.48 -8.53 -7.74 -7.57 0.16 -1.33
Eisosomes -2.83 1.28 -2.10 1.51 -3.11 -10.65 -7.08 -4.26 -1.28 -2.42 -6.05 -5.98 -5.66 0.68 -3.67
Endoplasmic Reticulum -2.40 -3.14 -2.10 0.02 2.91 -0.66 -0.90 -1.62 -1.41 0.39 -1.65 -3.19 -2.09 -0.96 2.92
Endosome -1.55 -4.83 0.04 2.97 8.87 -3.59 0.41 -1.71 0.22 -1.88 -3.33 -5.29 0.63 4.29 6.45
Golgi 0.87 2.69 6.89 9.58 9.48 -1.16 3.96 8.34 8.60 4.03 0.60 6.81 15.05 14.98 10.46
Lipid Particles -1.71 2.00 4.25 5.16 3.43 -5.02 1.35 -1.16 0.90 -1.49 -4.95 2.01 2.02 6.38 0.58
Mitochondria -5.01 1.79 -4.75 0.55 -9.69 -8.15 -5.00 -1.41 3.51 1.83 -10.79 -2.14 -9.04 0.77 -7.82
Mitotic Spindle 0.60 -4.55 -3.73 -7.01 0.65 -2.64 -5.01 -3.51 -2.90 -2.30 -1.91 -10.53 -7.44 -6.20 -1.24
None 0.60 0.89 2.76 2.38 1.99 -1.59 -0.43 0.71 1.85 0.99 -2.57 -2.81 -1.02 1.48 1.77
Nuclear Periphery 1.47 1.77 2.46 1.96 2.42 -3.92 -2.85 1.28 4.61 3.32 -1.59 -0.49 3.36 5.15 4.14
Nuclear Periphery Foci 0.86 0.37 2.44 2.09 3.24 -1.22 1.58 0.04 0.76 -0.82 -0.26 1.04 2.72 3.62 2.21
Nucleolus -0.56 -0.05 -3.75 -3.61 -3.97 -4.21 -4.81 -4.40 -2.41 -0.77 -4.43 -4.38 -6.65 -3.72 -3.43
Nucleus -1.13 -9.11 -6.10 -4.99 1.91 -1.28 -2.74 -4.09 -3.57 -2.56 -1.77 -9.43 -6.79 -5.98 1.01
Peroxisomes -1.73 1.35 -2.15 0.82 -5.53 -5.79 -5.63 -5.00 -2.48 -3.91 -4.86 -4.40 -8.42 -0.01 -6.56
Vacuole 0.57 -2.66 -0.42 -1.27 3.03 3.59 2.94 2.04 -0.00 0.24 4.50 1.37 3.70 -0.41 2.73
Vacuole Periphery 9.02 10.84 12.48 5.10 3.83 19.53* 11.61 17.51 4.17 7.31 21.76 22.13 26.46 6.21 7.13
Standard name
Human Ortholog
Description Mitochondrial inner membrane transporter; transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family
Localization
Cell Percentages mitochondrion (20%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Oac1

Oac1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Oac1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available