Standard name
Human Ortholog
Description Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO2 has a paralog, IGO1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.91 0.96 0.95 0.89 – 0.66 0.62 0.69 0.71 0.64 1.0 0.98 0.99 0.96 0.94 0.97 0.96 0.97 0.92 0.94 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 – 0.13 0.24 0.16 0.24 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.07 – 0.23 0.18 0.15 0.07 0.12 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 1 2 0 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 2 0 0 0 – 4 2 1 0 4 0 0 1 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 2 0 2 2 3 – 2 4 11 5 8 0 0 0 0 2 1 0 0 0 0 0 0
Cytoplasm 350 203 52 186 208 152 – 179 138 187 114 149 215 276 283 86 73 115 341 200 63 74 203 243
Endoplasmic Reticulum 1 0 1 5 1 0 – 6 5 10 5 3 1 1 0 2 1 4 0 0 0 1 0 4
Endosome 0 1 0 0 0 0 – 0 0 1 0 1 0 0 1 0 1 0 0 0 0 0 1 1
Golgi 1 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0 0
Mitochondria 7 1 1 2 2 13 – 35 53 44 38 63 0 0 0 0 2 1 3 0 0 0 3 0
Nucleus 2 1 2 1 0 0 – 3 4 4 2 11 1 1 1 1 1 2 1 1 0 1 1 2
Nuclear Periphery 0 0 0 0 0 0 – 2 2 1 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 – 2 2 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 1 2 10 12 – 61 39 40 11 28 0 5 3 3 4 1 2 0 2 1 2 2
Unique Cell Count 360 206 57 194 219 170 271 221 271 161 233 216 281 286 90 78 119 355 206 69 79 220 263
Labelled Cell Count 367 209 59 199 223 181 295 251 299 180 272 217 283 289 92 84 125 355 206 69 79 220 263


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.1 13.6 8.3 11.1 12.2 10.3 – 10.2 9.9 9.9 9.3 9.9 12.5 12.5 12.4 15.2 14.4 14.6 11.8 12.8 13.1
Std Deviation (1e-4) 1.7 1.8 1.8 2.6 2.6 2.7 – 2.7 3.7 2.5 2.7 2.5 2.1 2.0 1.7 4.3 3.1 3.6 2.4 2.5 2.6
Intensity Change (Log2) – – – 0.42 0.55 0.3 – 0.29 0.25 0.25 0.16 0.24 0.58 0.58 0.57 0.87 0.79 0.8 0.5 0.62 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 1.1 -0.4 – -3.8 -4.2 -3.4 -3.1 -4.0 3.8 2.8 3.5 1.1 0.5 1.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 2.5 3.8 2.9 3.7 4.1 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 3.6 3.0 2.7 0 2.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.2886 9.6244 8.4641 7.7161 7.5518 8.2242 6.6034 9.314 8.0289 7.5687 6.8749 8.3904 9.7758 12.5083 11.3851 11.726 10.2726 10.7204
Actin 0.0148 0.0004 0.0038 0.0035 0.0016 0.0006 0.0169 0.0003 0.0023 0.0001 0.0013 0.0019 0.0472 0.0001 0.0225 0.0004 0.0005 0.0105
Bud 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.001 0.0001 0.0002 0.0001 0.0008 0.0003 0.0005 0 0.0003 0 0.0001 0.0002
Bud Neck 0.0007 0.0004 0.002 0.0011 0.0005 0.0012 0.0026 0.0002 0.0004 0.0004 0.0006 0.0008 0.0013 0.0001 0.0003 0.0003 0.0005 0.0012
Bud Periphery 0.0003 0 0.0001 0.0001 0.0002 0.0001 0.0011 0 0.0002 0 0.0008 0.0001 0.0004 0 0.0002 0 0 0.0003
Bud Site 0.0016 0.0022 0.0017 0.0005 0.0004 0 0.0075 0.0002 0.0003 0 0.0004 0 0.0031 0 0.0014 0 0 0.0004
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0002 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Cytoplasm 0.8421 0.9756 0.9486 0.861 0.8884 0.936 0.7941 0.9724 0.9316 0.9898 0.8098 0.9495 0.8241 0.9938 0.9462 0.9946 0.9908 0.8811
Cytoplasmic Foci 0.0294 0.0069 0.0057 0.0199 0.0074 0.005 0.0368 0.0014 0.0044 0.001 0.0204 0.002 0.0252 0.0005 0.011 0.0012 0.0013 0.0084
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0006 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0037 0.001 0.001 0.0008 0.001 0.0011 0.004 0.0008 0.0012 0.0006 0.0018 0.0012 0.0023 0.0002 0.0006 0.0002 0.0007 0.0013
Endosome 0.0302 0.0009 0.004 0.0608 0.0131 0.0065 0.0428 0.0009 0.0127 0.0005 0.0819 0.0017 0.0088 0.0001 0.002 0.0001 0.0003 0.0078
Golgi 0.0118 0.0003 0.0026 0.0075 0.0086 0.0032 0.0123 0.0001 0.004 0 0.0083 0.0001 0.006 0 0.0023 0 0 0.0109
Lipid Particles 0.0054 0.0001 0.0011 0.0116 0.0332 0.0028 0.0154 0 0.0004 0 0.0031 0 0.0125 0 0.0013 0 0 0.0106
Mitochondria 0.0087 0.0001 0.004 0.0016 0.0336 0.0162 0.0157 0.0001 0.0225 0 0.0076 0.0002 0.0065 0 0.0012 0 0.0001 0.0027
None 0.0034 0.0014 0.0016 0.001 0.0012 0.0012 0.0032 0.0011 0.0016 0.0008 0.0013 0.0018 0.0083 0.0009 0.0015 0.0011 0.0008 0.0025
Nuclear Periphery 0.0072 0.0007 0.0012 0.0007 0.0006 0.0015 0.0053 0.0017 0.0016 0.0008 0.0084 0.0016 0.0035 0.0003 0.0006 0.0002 0.0005 0.0182
Nucleolus 0.0004 0 0.0001 0.0002 0 0 0.0005 0 0 0 0.0002 0 0.0003 0 0 0 0 0.0005
Nucleus 0.0289 0.0088 0.0135 0.0056 0.0039 0.0225 0.0221 0.0196 0.0135 0.0054 0.0107 0.0374 0.0182 0.0038 0.0045 0.0017 0.004 0.0281
Peroxisomes 0.0052 0.0005 0.0075 0.0206 0.0043 0.0004 0.0086 0.0001 0.0005 0 0.0066 0.0001 0.0191 0 0.0027 0 0.0001 0.0111
Punctate Nuclear 0.0025 0.0003 0.0006 0.0005 0.0003 0.0006 0.0021 0.0005 0.0005 0.0002 0.0004 0.0008 0.0115 0.0001 0.0012 0.0001 0.0001 0.0026
Vacuole 0.0018 0.0002 0.0005 0.0025 0.0009 0.0005 0.0057 0.0004 0.0013 0.0002 0.0267 0.0004 0.0004 0 0.0001 0 0.0001 0.0006
Vacuole Periphery 0.001 0 0.0002 0.0005 0.0004 0.0004 0.0017 0.0001 0.0005 0.0001 0.0091 0.0002 0.0002 0 0.0001 0 0 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.6501 17.4359 24.0281 39.302 39.7392 38.9877 28.9627 36.546 36.8225 49.478
Translational Efficiency 2.3033 2.8076 2.037 1.3472 1.5282 2.5377 2.4242 1.9675 2.1753 1.7854

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1027 1462 213 1231 38 1775 1935 1572 1065 3237 2148 2803

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 951.59 1115.69 1419.42 1346.47 911.59 1118.32 1314.91 1291.68 950.16 1117.13 1325.27 1315.74
Standard Deviation 149.55 182.21 183.22 226.73 159.82 170.36 188.71 193.02 150.12 175.82 190.75 210.26
Intensity Change Log 2 — 0.229524 0.576890 0.500770 — 0.294876 0.528507 0.502792 — 0.261869 0.553420 0.501760

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000136 0.000166 0.001819 0.003561 0.000843 0.000201 0.000211 0.003145 0.000162 0.000185 0.000370 0.003328
Bud Neck 0.000812 0.001162 0.004058 0.005540 0.001184 0.001724 0.001258 0.003845 0.000825 0.001470 0.001535 0.004589
Bud Site 0.003116 0.004642 0.008257 0.023800 0.013227 0.005282 0.006154 0.030621 0.003476 0.004993 0.006363 0.027625
Cell Periphery 0.000072 0.000037 0.000289 0.000168 0.000456 0.000046 0.000064 0.000081 0.000086 0.000042 0.000087 0.000119
Cytoplasm 0.803426* 0.833623* 0.539201* 0.574122* 0.721144* 0.822888* 0.670451* 0.665181* 0.800490* 0.827737* 0.657436* 0.625190*
Cytoplasmic Foci 0.057776 0.020906 0.017418 0.031579 0.090340 0.039333 0.004716 0.028290 0.058938 0.031011 0.005976 0.029734
Eisosomes 0.000014 0.000006 0.000022 0.000016 0.000029 0.000010 0.000004 0.000015 0.000015 0.000008 0.000006 0.000015
Endoplasmic Reticulum 0.000884 0.000441 0.002374 0.001205 0.004043 0.000394 0.000270 0.000595 0.000997 0.000415 0.000479 0.000863
Endosome 0.002212 0.001302 0.002964 0.015684 0.004186 0.003434 0.000274 0.019007 0.002282 0.002471 0.000541 0.017548
Golgi 0.000305 0.000590 0.000384 0.023084 0.002141 0.002858 0.000248 0.009677 0.000371 0.001833 0.000261 0.015565
Lipid Particles 0.001073 0.000329 0.003358 0.001125 0.001579 0.000436 0.000254 0.004253 0.001091 0.000388 0.000562 0.002879
Mitochondria 0.002215 0.000198 0.000351 0.014783 0.002968 0.001937 0.000233 0.005269 0.002242 0.001152 0.000245 0.009447
Mitotic Spindle 0.000646 0.002753 0.004700 0.074113 0.001178 0.003103 0.005164 0.053221 0.000665 0.002945 0.005118 0.062396
None 0.006115 0.006511 0.005144 0.008125 0.003165 0.004990 0.009896 0.008454 0.006010 0.005677 0.009425 0.008309
Nuclear Periphery 0.000107 0.000129 0.000846 0.000871 0.000116 0.000106 0.000275 0.000393 0.000108 0.000116 0.000331 0.000603
Nuclear Periphery Foci 0.003146 0.001735 0.006210 0.004655 0.004710 0.001652 0.000919 0.003069 0.003202 0.001689 0.001444 0.003765
Nucleolus 0.000358 0.000331 0.000617 0.000293 0.001669 0.000244 0.000114 0.000117 0.000405 0.000283 0.000164 0.000194
Nucleus 0.073284 0.097904 0.311588* 0.082744 0.102984 0.068072 0.278844* 0.082587 0.074344 0.081545 0.282091* 0.082656
Peroxisomes 0.000336 0.000748 0.000250 0.002332 0.000883 0.000998 0.000421 0.003222 0.000355 0.000885 0.000404 0.002831
Vacuole 0.043724 0.026178 0.089954 0.108310 0.029051 0.041691 0.020187 0.075751 0.043200 0.034684 0.027106 0.090050
Vacuole Periphery 0.000242 0.000311 0.000195 0.023890 0.014103 0.000599 0.000042 0.003209 0.000737 0.000469 0.000057 0.012291

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.79 -2.97 -2.55 -2.46 1.72 2.91 2.23 -0.05 -2.69 -2.08 -0.42 -4.09 -3.73 -3.65 -1.71
Bud Neck -1.18 -7.96 -6.35 -4.24 4.02 -1.26 -1.40 -2.13 -1.02 -1.16 -2.92 -5.96 -6.75 -3.37 -1.27
Bud Site -1.55 -4.68 -6.70 -4.90 0.38 3.31 3.44 1.27 -3.91 -4.04 -2.13 -2.30 -8.24 -6.15 -4.80
Cell Periphery 6.79 -10.67 -5.82 -10.52 8.83 3.50 3.18 3.26 -7.82 1.34 6.65 -3.14 -1.37 -12.96 2.46
Cytoplasm -1.99 20.53 30.18 33.91 -4.13 -2.09 2.75 2.85 26.91 0.61 -2.55 28.19 31.15 42.23 3.68
Cytoplasmic Foci 12.21 11.83 15.38 5.12 2.30 2.11 3.64 3.24 13.79 -10.39 9.30 19.04 16.12 14.16 -10.23
Eisosomes 7.58 -6.04 -2.07 -11.57 5.28 3.43 3.90 2.70 -4.56 -9.93 6.22 4.54 -0.48 -10.94 -8.04
Endoplasmic Reticulum 2.70 -5.28 2.27 -1.14 6.54 3.77 3.69 3.84 1.37 4.34 3.90 1.84 3.97 -0.17 5.82
Endosome 3.39 -1.81 -0.48 -2.91 1.26 0.87 3.15 0.99 0.27 -4.20 0.19 6.82 -0.90 -1.01 -5.33
Golgi -0.97 -2.88 -3.05 -2.73 -2.86 -0.39 2.76 0.92 1.41 -2.66 -3.16 2.90 -3.68 -0.58 -4.11
Lipid Particles 2.10 -2.39 -2.33 -12.64 1.76 2.64 2.17 0.26 -2.89 -2.31 2.10 0.30 -1.49 -6.05 -2.88
Mitochondria 1.60 1.37 0.16 -1.98 -1.65 0.57 1.26 1.00 1.40 -1.77 0.92 1.56 0.68 -0.52 -2.35
Mitotic Spindle -2.47 -2.86 -8.03 -7.03 -3.78 -2.06 -4.22 -7.02 -6.16 -4.88 -3.62 -5.71 -10.78 -9.31 -7.19
None -0.17 1.07 1.00 1.73 -0.30 -1.87 -7.47 -2.87 -1.20 7.00 0.53 -2.71 0.69 0.32 7.74
Nuclear Periphery -2.04 -13.90 -22.46 -18.77 3.30 -0.03 -12.81 -10.61 -16.49 1.34 -1.87 -26.25 -26.72 -24.52 -2.34
Nuclear Periphery Foci 3.91 -4.97 1.62 -2.87 5.88 1.65 1.91 1.66 0.09 -2.47 4.75 4.44 3.61 -1.99 -1.44
Nucleolus -0.14 -0.21 1.21 0.82 1.01 1.20 1.29 1.27 2.10 -1.02 0.54 1.84 1.76 1.27 -0.53
Nucleus -7.83 -16.48 -8.77 -1.12 14.06 0.96 -5.84 -0.31 -12.51 35.95 -3.36 -44.31 -10.24 -9.58 40.77
Peroxisomes -0.96 -0.28 -0.71 0.52 -0.29 -0.28 2.45 0.36 0.68 -2.57 -2.24 1.07 -1.83 0.84 -2.60
Vacuole 2.24 -7.79 -31.31 -32.76 -8.42 -1.84 -2.94 -15.14 -26.77 -24.12 0.31 -6.90 -38.46 -41.62 -32.65
Vacuole Periphery -0.25 0.40 -2.86 -2.83 -2.88 1.06 1.10 1.06 0.30 -2.60 0.67 1.45 -1.23 -2.60 -3.55
Standard name
Human Ortholog
Description Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO2 has a paralog, IGO1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Igo2

Igo2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Igo2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available