ORF
Human Ortholog
Description Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 1.0 0.98 0.98 0.97 0.93 0.95 0.93 0.95 0.92 0.98 1.0 0.99 0.97 0.98 0.98 0.88 0.89 0.83 0.87 0.83 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0.06 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.13 0.13 0 0 0 0.08 0.06 0.05 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 1 0 0 1 4
Bud 2 0 2 1 2 1 1 3 3 6 5 8 1 0 0 0 0 0 9 4 1 4 10 17
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 2 0 0 2 3
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 2 1 3 1 1 1 2 2 1 0 2 3 7 7 0 0 0 0 0 0
Cytoplasm 836 362 134 93 173 181 229 244 143 254 175 239 301 478 524 178 162 210 745 326 113 205 321 324
Endoplasmic Reticulum 2 0 0 0 0 0 0 0 0 0 0 1 2 0 2 2 0 1 1 0 1 0 3 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 14 2 2 5 7 11
Golgi 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 5 0 0 1 3 2
Mitochondria 13 4 6 3 2 2 3 4 10 16 14 18 1 1 0 0 0 0 7 2 0 0 3 2
Nucleus 0 0 0 0 0 2 1 1 0 1 1 3 0 1 1 1 1 2 3 2 0 2 2 2
Nuclear Periphery 1 2 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 1 0 2 1 1 3 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 6 2 0 0 3 5
Vac/Vac Membrane 8 1 0 0 2 0 2 12 3 8 1 9 2 1 0 24 21 8 36 14 10 13 20 49
Unique Cell Count 857 367 137 93 176 185 236 261 150 272 184 261 306 480 527 183 165 215 848 366 137 237 386 430
Labelled Cell Count 866 370 143 97 182 188 242 267 161 289 198 282 310 481 529 208 192 231 848 366 137 237 386 430


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.1 3.9 4.4 6.7 6.0 6.4 6.5 5.6 6.3 5.7 6.1 5.8 6.7 6.5 25.5 26.4 23.7 5.7 7.1 7.4
Std Deviation (1e-4) 1.0 1.0 1.4 1.3 1.4 1.3 1.8 1.7 1.9 2.2 1.9 2.0 1.1 1.4 1.2 4.6 4.5 3.9 1.4 2.9 2.5
Intensity Change (Log2) – – – 0.18 0.78 0.62 0.72 0.74 0.53 0.68 0.54 0.63 0.57 0.77 0.72 2.7 2.75 2.6 0.54 0.86 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 0.3 0 -0.4 -1.9 -1.1 -1.9 -1.3 -2.4 0.4 2.0 1.8 -0.3 0.2 -0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.8 0.6 1.2 1.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 4.4 4.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7186 2.0844 1.3743 0.9007 0.811 1.6663 1.0859 0.5552 0.7136 0.7185 0.8082 0.8451 1.9346 2.4569 2.0703 2.1618 1.8316 2.1623
Actin 0.0193 0.0001 0.0003 0.0026 0.0003 0.0006 0.0457 0.0035 0.0227 0.0133 0.0627 0.0229 0.0034 0.0062 0.03 0.0001 0.0002 0.0077
Bud 0.0002 0.0015 0.0013 0.0001 0.0001 0.0003 0.0023 0.0047 0.0009 0.0044 0.0122 0.0012 0.0001 0.0016 0.0008 0.0001 0.0002 0.0008
Bud Neck 0.0014 0.0001 0.0002 0.0002 0.0001 0.002 0.0109 0.0002 0.0103 0.0005 0.0006 0.0073 0.0115 0.0002 0.0007 0.0001 0.0004 0.0011
Bud Periphery 0.0003 0.0014 0.0014 0 0 0.0002 0.0055 0.0063 0.001 0.0073 0.0205 0.0024 0.0002 0.0016 0.0013 0.0001 0.0001 0.0023
Bud Site 0.0031 0.0012 0.0013 0 0.0001 0.0002 0.012 0.013 0.0032 0.0285 0.0036 0.0015 0.0103 0.0086 0.0024 0 0.0006 0.001
Cell Periphery 0.0002 0.0001 0.0001 0 0 0.0001 0.0006 0.0002 0.0002 0.0008 0.0003 0.0002 0.0001 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.515 0.6911 0.6535 0.7083 0.696 0.7006 0.2904 0.5164 0.4287 0.5431 0.4509 0.5249 0.3026 0.3854 0.3283 0.257 0.3498 0.4155
Cytoplasmic Foci 0.0108 0.0057 0.0095 0.0087 0.0141 0.0162 0.0517 0.0098 0.0323 0.0155 0.0436 0.0205 0.0071 0.0051 0.0075 0.0043 0.0544 0.0173
Eisosomes 0.0004 0 0 0.0001 0 0 0.0006 0.0001 0.0001 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0064 0.0019 0.0025 0.001 0.0062 0.0022 0.0064 0.0017 0.0046 0.0036 0.0043 0.0036 0.0013 0.0009 0.0014 0.0008 0.0007 0.0009
Endosome 0.0096 0.0048 0.0056 0.0015 0.0118 0.009 0.0458 0.002 0.0395 0.0656 0.0501 0.0229 0.0059 0.001 0.0037 0.0009 0.0135 0.0074
Golgi 0.0025 0.0001 0.0002 0.0006 0.0003 0.0007 0.0203 0.0004 0.0104 0.0129 0.0281 0.022 0.0011 0.0005 0.0018 0.0001 0.0019 0.0021
Lipid Particles 0.0026 0.0001 0.0005 0.0002 0.0002 0.0005 0.0255 0.0003 0.0028 0.0062 0.0057 0.007 0.0017 0.0004 0.0015 0.0001 0.0059 0.0032
Mitochondria 0.0013 0.0001 0.0005 0.0003 0.0002 0.0003 0.0155 0.0004 0.0052 0.0651 0.0349 0.0128 0.0002 0.0019 0.0008 0.0001 0.0004 0.0044
None 0.4085 0.2849 0.3138 0.272 0.2634 0.2594 0.4071 0.4348 0.4235 0.2081 0.2508 0.3316 0.6455 0.5788 0.6111 0.7341 0.5427 0.5157
Nuclear Periphery 0.0042 0.0009 0.0014 0.0004 0.0018 0.001 0.0068 0.0006 0.0029 0.0031 0.0024 0.0016 0.0032 0.0003 0.0011 0.0003 0.0007 0.0023
Nucleolus 0.0006 0.0001 0.0002 0 0 0.0001 0.0014 0.0001 0.0001 0.0008 0.0005 0.0002 0.0002 0.0001 0.0002 0 0.0007 0.0008
Nucleus 0.0032 0.0016 0.0029 0.0017 0.0017 0.0029 0.0047 0.0014 0.0016 0.0029 0.0028 0.0017 0.0013 0.0011 0.002 0.0009 0.0022 0.0023
Peroxisomes 0.0018 0 0.0001 0.0008 0.0001 0.0001 0.0229 0.0013 0.0045 0.0013 0.0161 0.0097 0.0006 0.0047 0.0023 0.0001 0.0037 0.0038
Punctate Nuclear 0.0062 0.0004 0.0008 0.0006 0.0005 0.0013 0.0123 0.0008 0.0012 0.0016 0.0025 0.0027 0.0027 0.0007 0.0019 0.0003 0.0199 0.0104
Vacuole 0.0018 0.0036 0.0036 0.0005 0.0027 0.0021 0.0087 0.002 0.0034 0.0127 0.0057 0.0023 0.0007 0.0007 0.0009 0.0004 0.0017 0.0007
Vacuole Periphery 0.0004 0.0003 0.0004 0.0001 0.0004 0.0003 0.0029 0.0002 0.001 0.0025 0.0014 0.0007 0.0002 0.0001 0.0002 0.0001 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.9871 2.8591 14.8508 20.6882 10.3668 15.9825 15.1767 32.193 21.3875 18.704
Translational Efficiency 3.4494 8.8949 2.1 1.7933 2.1001 7.3047 3.9294 1.6122 2.471 2.07

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
274 1327 224 1087 1693 1824 1772 1998 1967 3151 1996 3085

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 683.85 705.90 973.53 997.65 726.14 751.00 886.59 876.60 720.25 732.01 896.35 919.25
Standard Deviation 102.49 99.82 134.80 194.17 88.52 94.73 109.65 188.32 91.77 99.43 116.05 198.99
Intensity Change Log 2 — 0.045784 0.509546 0.544854 — 0.048565 0.288019 0.271671 — 0.047217 0.399711 0.410628

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000185 0.000331 0.000674 0.000541 0.000066 0.000215 0.000140 0.000513 0.000083 0.000264 0.000200 0.000523
Bud Neck 0.020901 0.030484 0.001902 0.006231 0.010373 0.034794 0.003328 0.005749 0.011840 0.032979 0.003168 0.005919
Bud Site 0.008918 0.018270 0.003573 0.026537 0.003976 0.013599 0.011659 0.018250 0.004664 0.015566 0.010752 0.021170
Cell Periphery 0.000125 0.000098 0.000228 0.000101 0.000079 0.000084 0.000088 0.000164 0.000085 0.000090 0.000104 0.000142
Cytoplasm 0.443865* 0.478065* 0.331434* 0.566906* 0.557532* 0.495069* 0.564787* 0.621298* 0.541698* 0.487908* 0.538599* 0.602133*
Cytoplasmic Foci 0.235527* 0.218222* 0.015241 0.017665 0.155932 0.197819* 0.009074 0.017895 0.167019 0.206411* 0.009766 0.017814
Eisosomes 0.000155 0.000103 0.000092 0.000030 0.000090 0.000123 0.000028 0.000027 0.000099 0.000115 0.000035 0.000028
Endoplasmic Reticulum 0.000393 0.000574 0.006993 0.001662 0.000392 0.000286 0.001275 0.000714 0.000392 0.000407 0.001916 0.001048
Endosome 0.034886 0.029593 0.005985 0.004382 0.014262 0.024395 0.001775 0.003178 0.017135 0.026584 0.002247 0.003602
Golgi 0.000802 0.003389 0.000155 0.000802 0.001095 0.003594 0.000068 0.000834 0.001054 0.003507 0.000078 0.000823
Lipid Particles 0.003465 0.004944 0.003574 0.001106 0.004078 0.003852 0.000772 0.001179 0.003993 0.004312 0.001086 0.001154
Mitochondria 0.004976 0.007536 0.000708 0.000754 0.003780 0.007284 0.000683 0.001853 0.003947 0.007390 0.000686 0.001466
Mitotic Spindle 0.000489 0.007268 0.001002 0.055112 0.003964 0.009262 0.014308 0.032881 0.003480 0.008422 0.012814 0.040714
None 0.000693 0.004341 0.006163 0.003772 0.001088 0.001556 0.005977 0.005377 0.001033 0.002729 0.005998 0.004812
Nuclear Periphery 0.000255 0.000364 0.001987 0.000973 0.000344 0.000247 0.001230 0.000864 0.000331 0.000296 0.001315 0.000902
Nuclear Periphery Foci 0.000546 0.000379 0.003702 0.002355 0.000262 0.000164 0.000828 0.000726 0.000302 0.000255 0.001150 0.001300
Nucleolus 0.003767 0.001751 0.000705 0.000308 0.000595 0.001001 0.000252 0.000361 0.001037 0.001317 0.000303 0.000343
Nucleus 0.062867 0.063338 0.475559* 0.134565* 0.108564 0.084928 0.305474* 0.202233* 0.102199 0.075836 0.324561* 0.178390*
Peroxisomes 0.005562 0.015281 0.001270 0.002842 0.002597 0.010826 0.000209 0.000948 0.003010 0.012702 0.000328 0.001615
Vacuole 0.170442 0.113436 0.138799 0.172738 0.130095 0.108933 0.077779 0.084689 0.135715 0.110829 0.084627 0.115713
Vacuole Periphery 0.001182 0.002233 0.000254 0.000618 0.000835 0.001969 0.000268 0.000266 0.000884 0.002080 0.000266 0.000390

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.19 -4.42 -1.81 -0.06 3.44 -5.96 -8.43 -4.18 -2.22 -2.79 -8.62 -7.95 -5.26 -1.95 -2.24
Bud Neck -2.13 4.34 3.92 11.45 -1.64 -12.28 9.23 6.53 15.50 -2.69 -13.08 9.77 7.59 19.29 -2.99
Bud Site -4.91 2.76 -1.61 2.76 -4.07 -7.75 -2.31 -6.32 0.66 -3.19 -10.66 -1.82 -7.01 2.37 -3.86
Cell Periphery 2.41 -5.67 3.82 2.13 8.44 -0.84 -0.24 -2.74 -2.48 -2.65 -0.76 -2.18 -2.21 -1.93 -1.02
Cytoplasm -1.85 8.30 1.05 4.53 -9.53 6.60 10.73 1.79 -4.98 -9.17 6.64 12.14 5.19 -1.63 -7.98
Cytoplasmic Foci 1.43 18.87 19.11 35.61 0.76 -7.11 37.15 34.25 38.86 -10.42 -7.60 41.16 39.03 52.54 -9.73
Eisosomes 3.31 2.22 7.81 13.62 10.06 -4.60 12.57 14.28 14.70 3.36 -2.70 12.06 16.04 19.61 7.64
Endoplasmic Reticulum -2.38 -9.96 -10.08 -8.59 8.76 1.87 -17.94 -9.60 -11.81 12.19 -0.29 -17.38 -13.32 -13.81 12.14
Endosome 1.39 7.63 8.97 16.08 6.86 -6.49 15.94 14.55 15.93 -1.64 -6.90 17.19 16.99 22.53 0.24
Golgi -6.71 5.19 4.16 8.54 -0.59 -5.20 3.83 2.11 7.21 -2.00 -6.78 4.22 2.73 10.40 -2.30
Lipid Particles -2.23 -0.81 4.72 7.23 6.23 0.26 7.63 4.97 4.64 -1.37 -0.76 7.50 6.06 7.57 -0.68
Mitochondria -0.64 1.15 1.21 6.25 0.77 -2.68 3.25 2.59 6.26 -1.62 -2.87 3.37 3.04 8.68 -1.21
Mitotic Spindle -4.80 -1.29 -7.64 -5.24 -7.43 -2.66 -3.39 -6.67 -4.40 -3.62 -3.24 -3.50 -8.98 -6.76 -5.46
None -4.50 -4.04 -10.97 -1.08 -1.00 -1.74 -13.42 -8.05 -6.14 1.51 -4.52 -14.05 -11.79 -4.59 1.21
Nuclear Periphery -1.86 -13.59 -17.32 -17.65 4.84 3.36 -31.10 -13.39 -14.59 9.55 1.33 -33.88 -20.97 -21.36 9.84
Nuclear Periphery Foci 1.89 -8.36 -8.53 -10.29 5.10 3.25 -10.92 -7.75 -8.76 0.15 1.43 -13.03 -12.48 -13.14 0.52
Nucleolus 1.20 1.96 2.18 8.59 3.43 -3.79 6.01 4.59 6.35 -1.07 -1.41 3.16 3.19 10.56 0.26
Nucleus -0.18 -19.72 -9.33 -15.90 16.05 5.62 -38.05 -16.62 -21.56 21.84 7.32 -42.43 -18.13 -27.09 28.38
Peroxisomes -5.18 3.14 3.52 11.12 0.39 -9.92 8.67 6.32 12.73 -2.72 -12.67 8.72 7.01 16.73 -2.25
Vacuole 4.73 -2.50 -12.09 -22.63 -6.99 4.70 -0.04 -8.39 -12.90 -7.98 5.73 -0.76 -16.98 -23.80 -15.29
Vacuole Periphery -3.20 4.20 4.45 8.47 1.30 -2.67 6.17 6.34 4.32 -0.13 -4.31 7.18 7.33 7.23 0.02
ORF
Human Ortholog
Description Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (26%), vacuole (10%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YHR138C

YHR138C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhr138c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available