ORF
Human Ortholog
Description Putative integral membrane protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0.06 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.86 0.94 0.91 0.94 0.92 0.9 0.84 0.8 0.8 0.83 0.8 0.99 0.96 0.94 0.92 0.88 0.94 0.43 0.44 0.37 0.51 0.53 0.59
Endoplasmic Reticulum – 0.12 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0.05 0.05 0 0.18 0.19 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0.05 0.1 0.07 0.07 0.14 0.17 0.17 0.16 0.2 0.22 0 0 0 0.05 0 0 0.15 0 0 0.06 0 0.05
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.1 0.06 0.25 0.25 0.36 0.35 0.32 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 1 0 0 0 1 3 0 3 0 2 1 0 1 0 0 2 0 1 3 4
Bud – 0 1 0 4 9 20 16 24 12 23 35 4 1 0 1 1 0 0 2 0 1 8 12
Bud Neck – 1 0 0 0 0 0 0 1 0 1 2 2 1 5 0 0 2 0 0 1 2 5 4
Bud Site – 0 0 0 0 0 0 1 2 1 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 2 1 1 1 1 5 0 5 5 3 9 0 1 1 0 0 0 0 1 2
Cytoplasm – 103 124 99 304 280 482 451 339 384 408 481 362 464 549 269 203 169 1 56 52 132 231 294
Endoplasmic Reticulum – 14 3 1 6 7 7 4 20 14 14 25 5 15 30 12 12 9 0 22 26 7 8 6
Endosome – 0 0 0 1 1 1 3 1 2 0 1 0 0 0 2 5 0 0 2 3 1 5 7
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 3 2
Mitochondria – 1 7 11 21 20 75 93 74 78 100 132 0 0 0 15 5 0 0 4 4 15 19 26
Nucleus – 2 0 1 0 0 4 7 2 4 3 1 1 0 0 1 3 1 0 0 0 1 0 1
Nuclear Periphery – 0 0 2 0 0 1 8 1 3 3 1 0 0 1 0 0 1 0 1 0 0 0 0
Nucleolus – 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 1 0 0 1 0 1 1 2 0 1 0 2 0 0 0 0 0 2 5 8
Vac/Vac Membrane – 0 4 2 4 5 13 19 13 27 21 21 2 0 4 13 22 11 1 32 51 90 138 116
Unique Cell Count 120 132 109 323 304 536 535 423 478 491 601 367 483 583 291 230 179 4 129 143 261 437 500
Labelled Cell Count 121 139 116 345 323 606 604 479 534 575 711 381 488 599 316 253 194 4 129 143 261 437 500


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.3 4.0 3.7 4.4 3.9 3.6 4.0 3.9 4.1 4.1 4.2 5.2 5.2 5.4 4.9 5.1 5.2 5.8 5.6 5.6
Std Deviation (1e-4) – 1.0 0.7 1.1 1.5 0.9 0.9 1.3 0.9 1.0 1.1 1.2 0.8 0.8 0.9 0.9 1.1 1.1 1.3 1.6 1.4
Intensity Change (Log2) – – – -0.11 0.12 -0.03 -0.14 0.01 -0.03 0.03 0.01 0.07 0.37 0.37 0.42 0.29 0.36 0.36 0.53 0.49 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 0.1 -0.7 -1.4 -2.9 -3.7 -3.7 -3.1 -3.8 2.9 1.1 0.1 -0.6 -1.8 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1.4 0 1.4 1.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -0.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 1.2 0.6 0 0 0 0 0.7 2.3 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4437 -0.1555 -0.3532 0.3252 -0.4295 -0.2055 -0.4444 0.645 0.0533 -0.042 -0.7458 -0.1857 -2.0964 -1.285 -1.7258 -1.6167 -2.2786 -1.9014
Actin 0.0175 0.0003 0.0055 0.0266 0.0244 0.0017 0.0215 0.0001 0.001 0 0.0095 0.0004 0.0061 0.0005 0.0004 0.0047 0.0003 0.0002
Bud 0.0002 0.0004 0.0032 0.0002 0.0002 0.0009 0.0018 0.0001 0.0001 0 0.0025 0.0001 0.0002 0 0.0001 0.0032 0 0
Bud Neck 0.001 0.0002 0.0008 0.0004 0.0024 0.002 0.0008 0 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0006 0.0001 0.0002
Bud Periphery 0.0002 0.0005 0.0032 0.0002 0.0003 0.0037 0.003 0.0001 0.0002 0.0001 0.0128 0.0002 0.0004 0 0.0001 0.0081 0 0
Bud Site 0.0038 0.002 0.0117 0.0003 0.0003 0.0002 0.0038 0.0002 0.0002 0.0001 0.0003 0 0.001 0.0002 0.0002 0.0008 0.0001 0
Cell Periphery 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0005 0.0001 0.0003 0.0001 0.0006 0.0001 0.0002 0.0001 0.0001 0.0005 0.0001 0
Cytoplasm 0.0949 0.1057 0.1042 0.0898 0.1881 0.1483 0.0652 0.162 0.1041 0.1165 0.1039 0.1391 0.1701 0.2369 0.1953 0.2351 0.2999 0.349
Cytoplasmic Foci 0.006 0.005 0.0042 0.014 0.004 0.009 0.0188 0.0104 0.0075 0.0455 0.0317 0.0112 0.0097 0.012 0.0169 0.0194 0.0147 0.0209
Eisosomes 0.0002 0.0002 0.0003 0.0002 0.0002 0 0.0006 0.0002 0.0005 0.0001 0.0003 0.0001 0.0003 0.0005 0.0002 0.0004 0 0.0001
Endoplasmic Reticulum 0.011 0.003 0.0109 0.0053 0.0043 0.0121 0.0055 0.0073 0.0042 0.0016 0.0071 0.0038 0.0121 0.0089 0.0095 0.0091 0.0028 0.0055
Endosome 0.0037 0.0025 0.0042 0.0161 0.0043 0.0052 0.0131 0.0234 0.0094 0.0447 0.0556 0.0113 0.006 0.0096 0.0059 0.0402 0.0041 0.013
Golgi 0.0007 0.0002 0.0004 0.0023 0.0018 0.0004 0.0017 0.0003 0.0005 0.0005 0.0011 0.0001 0.001 0.0002 0.0003 0.0009 0.0002 0.0002
Lipid Particles 0.0012 0.0006 0.0008 0.0022 0.0018 0.0005 0.0041 0.0007 0.0023 0.002 0.0218 0.0005 0.0028 0.0012 0.0033 0.0038 0.0007 0.002
Mitochondria 0.0005 0.0004 0.0008 0.002 0.0007 0.0004 0.0014 0.0005 0.0023 0.0009 0.0034 0.0003 0.0007 0.0002 0.0005 0.0019 0.0005 0.0002
None 0.8428 0.8676 0.8303 0.7978 0.7523 0.7992 0.833 0.7775 0.8376 0.7688 0.7207 0.815 0.778 0.7218 0.7552 0.6458 0.6726 0.6001
Nuclear Periphery 0.003 0.0015 0.0039 0.0247 0.0048 0.0061 0.0037 0.0019 0.0023 0.0008 0.0106 0.0006 0.0024 0.0024 0.0022 0.0064 0.0008 0.0013
Nucleolus 0.002 0.0006 0.001 0.0005 0.0006 0.0003 0.0006 0.0002 0.0005 0.0004 0.0004 0.0001 0.0005 0.0001 0.0003 0.0005 0.0001 0.0001
Nucleus 0.0027 0.0036 0.0039 0.0044 0.0018 0.0048 0.0021 0.0012 0.0013 0.0012 0.0023 0.0008 0.0025 0.0017 0.0014 0.0036 0.0008 0.0017
Peroxisomes 0.0005 0.0001 0.0002 0.0013 0.0005 0.0002 0.0042 0.0001 0.0006 0.0002 0.0005 0.0003 0.0006 0.0002 0.0027 0.0004 0.0002 0.0002
Punctate Nuclear 0.0066 0.0038 0.0021 0.0092 0.0058 0.0035 0.0064 0.0003 0.0023 0.0007 0.0008 0.0006 0.0024 0.0006 0.0023 0.0028 0.0007 0.0016
Vacuole 0.001 0.0013 0.0078 0.0017 0.001 0.0011 0.0076 0.0116 0.0176 0.0143 0.012 0.0137 0.0025 0.0025 0.0028 0.0089 0.0011 0.0035
Vacuole Periphery 0.0002 0.0002 0.0005 0.0009 0.0003 0.0003 0.0007 0.002 0.0051 0.0009 0.0019 0.0014 0.0003 0.0002 0.0003 0.0029 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
742 1333 296 1371 1323 1060 2455 1630 2065 2393 2751 3001

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 694.97 738.97 878.55 950.58 711.58 693.96 812.61 824.84 705.61 719.03 819.70 882.28
Standard Deviation 86.42 107.00 102.60 139.59 84.74 89.67 106.69 124.75 85.72 102.18 108.21 145.87
Intensity Change Log 2 — 0.088565 0.338174 0.451857 — -0.036173 0.191537 0.213088 — 0.026807 0.265852 0.336000

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000120 0.000259 0.000410 0.002512 0.000274 0.000645 0.000143 0.000241 0.000219 0.000430 0.000171 0.001278
Bud Neck 0.010268 0.021876 0.002607 0.008037 0.018197 0.039077 0.014354 0.007008 0.015348 0.029496 0.013090 0.007478
Bud Site 0.004515 0.010831 0.002375 0.024159 0.004216 0.014426 0.011074 0.021395 0.004324 0.012423 0.010138 0.022657
Cell Periphery 0.000249 0.000133 0.000337 0.000581 0.000160 0.000229 0.000160 0.000187 0.000192 0.000175 0.000179 0.000367
Cytoplasm 0.479972* 0.414955* 0.335751* 0.368376* 0.459704* 0.331963* 0.533353* 0.547397* 0.466986* 0.378193* 0.512091* 0.465612*
Cytoplasmic Foci 0.208562* 0.203489* 0.042401 0.040675 0.215879* 0.316148* 0.016586 0.036801 0.213249* 0.253392* 0.019364 0.038571
Eisosomes 0.000210 0.000154 0.000114 0.000145 0.000251 0.000471 0.000034 0.000062 0.000236 0.000294 0.000042 0.000100
Endoplasmic Reticulum 0.012089 0.008884 0.020544 0.079450 0.007241 0.005433 0.013316 0.004869 0.008983 0.007355 0.014094 0.038941
Endosome 0.021676 0.029783 0.012694 0.010029 0.023332 0.045999 0.002445 0.004170 0.022737 0.036966 0.003547 0.006847
Golgi 0.001506 0.003957 0.000143 0.001223 0.002654 0.008538 0.000077 0.000385 0.002242 0.005986 0.000084 0.000768
Lipid Particles 0.020432 0.013649 0.009576 0.012403 0.015123 0.027077 0.002352 0.002862 0.017031 0.019597 0.003129 0.007221
Mitochondria 0.002776 0.006404 0.000306 0.000943 0.004127 0.014231 0.000662 0.000997 0.003642 0.009871 0.000623 0.000972
Mitotic Spindle 0.000713 0.005035 0.000461 0.016087 0.001357 0.003108 0.003720 0.011708 0.001126 0.004181 0.003369 0.013709
None 0.003926 0.001223 0.002218 0.002276 0.001104 0.000545 0.002331 0.005509 0.002118 0.000923 0.002319 0.004032
Nuclear Periphery 0.001452 0.001293 0.007827 0.005516 0.002089 0.000954 0.005471 0.002794 0.001860 0.001143 0.005725 0.004038
Nuclear Periphery Foci 0.001670 0.001412 0.026263 0.031864 0.000856 0.001100 0.004666 0.003549 0.001148 0.001274 0.006990 0.016484
Nucleolus 0.000827 0.001430 0.001010 0.000387 0.000876 0.000879 0.000352 0.000347 0.000858 0.001186 0.000422 0.000365
Nucleus 0.092885 0.088337 0.365704* 0.097870 0.109047 0.060696 0.330838* 0.211652* 0.103240 0.076093 0.334590* 0.159671*
Peroxisomes 0.001866 0.006880 0.000605 0.001215 0.004273 0.019563 0.000248 0.000561 0.003408 0.012498 0.000286 0.000860
Vacuole 0.133125 0.177351 0.168371 0.295492* 0.128191 0.106237 0.057632 0.136692 0.129964 0.145851 0.069547 0.209239*
Vacuole Periphery 0.001161 0.002666 0.000282 0.000761 0.001047 0.002683 0.000188 0.000815 0.001088 0.002674 0.000198 0.000790

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.64 -5.50 -2.21 -2.00 -1.70 -2.82 1.07 0.18 4.59 -2.70 -2.65 0.38 -2.07 -1.40 -2.21
Bud Neck -5.09 3.97 1.42 8.64 -6.11 -6.66 2.48 7.59 11.20 4.74 -7.58 1.84 7.13 14.07 4.31
Bud Site -3.86 1.40 -6.44 -3.69 -8.09 -5.65 -4.39 -5.59 -0.36 -2.34 -7.10 -4.12 -8.64 -2.79 -4.44
Cell Periphery 3.91 -2.16 -3.96 -8.59 -2.12 -4.43 0.32 0.12 4.00 -0.17 1.31 1.07 -4.32 -5.85 -5.30
Cytoplasm 5.50 10.28 11.43 6.54 -2.20 12.22 -5.16 -2.65 -14.82 2.19 11.35 -2.33 5.88 -5.71 8.55
Cytoplasmic Foci 0.69 24.31 27.51 31.51 0.94 -12.00 37.76 33.99 42.15 -9.58 -6.87 47.46 43.52 50.34 -10.56
Eisosomes 4.19 6.29 6.48 3.03 -0.21 -5.48 15.46 13.12 10.71 -8.16 -2.84 19.20 13.87 11.31 -10.67
Endoplasmic Reticulum 1.39 -2.29 -7.96 -9.37 -6.49 1.03 -2.91 2.15 0.82 5.75 1.23 -2.56 -6.96 -8.06 -5.11
Endosome -3.19 5.42 8.68 11.91 4.01 -7.60 13.46 12.48 16.56 -2.47 -7.37 16.78 14.77 20.48 -5.91
Golgi -3.63 9.36 2.18 4.40 -1.63 -6.61 6.42 5.83 10.47 -2.32 -6.51 8.21 5.77 10.46 -2.35
Lipid Particles 3.24 5.80 5.50 3.25 -0.94 -5.11 11.78 11.45 11.73 -0.73 -1.75 14.41 11.14 12.12 -7.88
Mitochondria -2.07 1.96 1.59 4.83 -3.44 -4.40 3.32 3.17 6.59 -0.62 -4.49 3.75 3.52 8.14 -1.32
Mitotic Spindle -2.62 -0.02 -5.61 -2.96 -5.59 -1.26 -2.20 -4.40 -3.20 -3.07 -2.66 -2.62 -6.90 -4.16 -4.92
None 2.14 1.28 1.39 -1.97 0.32 2.92 -5.85 -4.25 -5.08 -2.76 2.51 -0.36 -1.89 -5.26 -2.28
Nuclear Periphery 0.46 -8.23 -6.18 -6.44 2.14 1.72 -3.84 -0.94 -3.39 3.29 1.64 -5.58 -3.88 -6.82 2.36
Nuclear Periphery Foci 0.88 -7.42 -15.34 -15.65 -0.42 -0.84 -7.55 -5.49 -4.48 1.73 -0.73 -9.88 -15.89 -15.66 -7.15
Nucleolus -2.29 -0.79 2.90 4.10 4.56 -0.19 2.66 2.50 3.96 -0.65 -1.94 3.18 3.43 5.25 0.59
Nucleus 0.90 -17.41 -4.46 -5.59 15.88 11.61 -39.72 -19.78 -28.62 14.81 8.39 -45.76 -17.66 -24.82 27.88
Peroxisomes -6.08 5.23 2.69 7.20 -0.75 -9.32 9.82 9.22 12.06 -2.93 -10.27 11.27 8.87 14.02 -2.27
Vacuole -5.85 -4.02 -22.91 -19.42 -12.29 3.67 10.00 -9.11 -12.05 -17.83 -3.55 8.99 -23.03 -20.11 -31.07
Vacuole Periphery -3.19 5.75 3.12 4.69 -1.46 -4.88 10.37 2.22 5.48 -1.58 -5.39 11.46 3.67 6.87 -2.34
ORF
Human Ortholog
Description Putative integral membrane protein of unknown function
Localization
Cell Percentages cytoplasm (13%), vacuole (10%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YHR140W

YHR140W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhr140w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available