Standard name
Human Ortholog
Description Nucleolar protein of unknown function; positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis; SPO12 has a paralog, BNS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.55 0.62 0.67 0.77 0.66 0.67 0.71 – 0.64 0.61 0.57 0.61 0.76 0.78 0.78 0.52 0.55 0.42 0.4 0.46 0.58 0.59 0.86 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.3 0.07 – 0.36 0.35 0.46 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.34 0.27 0.26 0.17 0.23 0.22 0.2 – 0.14 0.11 0.12 0.09 0.16 0.17 0.14 0.33 0.29 0.34 0.37 0.31 0.23 0.22 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Vac/Vac Membrane 0.11 0.1 0.07 0.08 0.11 0 0.05 – 0 0 0 0 0.1 0.07 0.11 0.19 0.18 0.23 0.05 0.08 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 4
Bud 0 0 0 0 0 8 3 – 3 5 10 8 1 1 0 0 0 0 16 8 1 13 13 12
Bud Neck 0 0 0 0 0 1 4 – 2 5 3 4 0 0 0 0 0 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 2 0 – 1 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 2 – 0 0 0 2 0 0 0 0 3 2 0 0 0 0 1 2
Cytoplasm 157 89 58 204 221 176 324 – 224 231 204 253 142 459 464 123 122 97 120 70 63 180 455 482
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 1 0 1 0 0 0 8 4 10 0 0 0 1 0 0
Endosome 0 2 0 1 3 0 6 – 0 1 0 3 1 7 3 0 7 4 3 1 0 1 3 1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 0 1 1 4 1 1 1 1 3
Mitochondria 4 4 2 8 11 78 32 – 126 132 163 168 3 1 2 2 3 7 6 2 3 8 16 14
Nucleus 97 38 22 44 77 57 93 – 48 41 41 38 30 100 81 77 65 79 114 47 25 66 12 1
Nuclear Periphery 0 0 0 0 1 0 0 – 0 2 3 1 0 0 2 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 1 0 – 0 1 2 3 0 0 0 1 0 1 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 4 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 0 0 0 0 10 – 5 4 1 5 0 0 0 0 0 1 19 6 3 12 7 7
Vac/Vac Membrane 31 15 6 22 37 3 21 – 7 8 7 8 18 43 64 46 40 53 14 11 5 12 3 2
Unique Cell Count 283 143 86 266 334 262 455 350 376 355 414 187 592 597 236 223 231 305 152 108 306 531 547
Labelled Cell Count 291 148 88 279 350 326 495 416 431 435 496 195 613 620 257 245 255 305 152 108 306 531 547


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.6 4.8 5.0 5.4 3.8 4.5 – 4.0 4.0 3.8 3.9 5.3 5.2 5.2 6.5 7.0 6.8 4.8 4.5 4.4
Std Deviation (1e-4) 1.3 1.1 0.8 1.7 1.4 1.5 1.4 – 1.7 1.2 1.3 1.4 1.7 1.5 1.4 1.8 1.9 1.6 1.6 1.6 1.4
Intensity Change (Log2) – – – 0.07 0.18 -0.33 -0.09 – -0.24 -0.26 -0.33 -0.29 0.14 0.13 0.12 0.44 0.56 0.51 0.01 -0.09 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.7 2.4 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8627 -0.2793 -0.2289 0.274 0.5728 -0.1854 -0.2379 0.7126 0.6627 1.2085 1.7266 1.1831 1.2595 2.8691 2.4553 2.8477 3.2208 2.5717
Actin 0.0246 0.0001 0.0278 0.0003 0.0186 0.0019 0.0043 0.0008 0.0016 0.0004 0.0024 0.0008 0.0183 0.0002 0.0107 0.0286 0.0041 0.0005
Bud 0.0027 0.0012 0.0021 0.0003 0.0009 0.0011 0.0033 0.0101 0.0006 0.0001 0.0006 0.001 0.0062 0.0016 0.0002 0.0002 0.0001 0.0009
Bud Neck 0.0027 0.001 0.0033 0.0005 0.0002 0.0054 0.0039 0.001 0.0006 0.0005 0.0004 0.0075 0.0041 0.0002 0.0002 0.0004 0.0007 0.0018
Bud Periphery 0.0032 0.0006 0.0028 0.0004 0.0012 0.0014 0.0029 0.0039 0.0006 0.0001 0.0015 0.0021 0.0051 0.001 0.0002 0.0002 0.0001 0.0005
Bud Site 0.004 0.0065 0.0039 0.0001 0.0004 0.0004 0.0051 0.0134 0.0013 0.0002 0.001 0.0006 0.0125 0.0016 0.0007 0.0006 0.0004 0.0002
Cell Periphery 0.0003 0.0003 0.0003 0 0 0.0001 0.0004 0.0004 0.0001 0 0.0001 0.0002 0.0007 0.0002 0.0001 0 0 0
Cytoplasm 0.2 0.1823 0.1505 0.0291 0.0114 0.1839 0.2912 0.2357 0.2459 0.042 0.0057 0.2986 0.1542 0.1472 0.2044 0.0108 0.0281 0.2707
Cytoplasmic Foci 0.015 0.0058 0.0155 0.0002 0.0013 0.0122 0.0184 0.0068 0.0096 0.003 0.004 0.0119 0.0126 0.0016 0.0052 0.0116 0.0348 0.0048
Eisosomes 0.0003 0.0001 0.0005 0 0.0002 0 0.0004 0.0001 0.0001 0 0.0001 0 0.0006 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0069 0.0029 0.0081 0.0038 0.0014 0.0027 0.008 0.0023 0.003 0.0005 0.001 0.003 0.0071 0.0018 0.0086 0.0005 0.0022 0.0027
Endosome 0.0172 0.0034 0.013 0.0012 0.0046 0.0039 0.0131 0.0019 0.0065 0.0008 0.024 0.0065 0.0102 0.001 0.0033 0.0057 0.0087 0.002
Golgi 0.0027 0.0001 0.0024 0 0.0017 0.0003 0.0018 0.0002 0.001 0.0001 0.0038 0.0005 0.0028 0.0001 0.0004 0.0049 0.0011 0.0001
Lipid Particles 0.0078 0.0012 0.0045 0 0.0021 0.0008 0.0055 0.0002 0.0049 0.0002 0.0177 0.0006 0.0085 0.0001 0.0004 0.0118 0.0017 0.0001
Mitochondria 0.0042 0.0004 0.0027 0.0022 0.0029 0.0007 0.0027 0.0009 0.0063 0.0003 0.0075 0.0015 0.0034 0.0006 0.0015 0.0009 0.0006 0.0003
None 0.5616 0.5551 0.3737 0.0176 0.039 0.3239 0.4383 0.3165 0.2336 0.0088 0.0026 0.2125 0.6249 0.5556 0.3724 0.0061 0.0216 0.399
Nuclear Periphery 0.0097 0.0059 0.0262 0.0466 0.0375 0.0128 0.0133 0.0088 0.011 0.0106 0.0145 0.0059 0.0095 0.0046 0.0123 0.0034 0.014 0.0033
Nucleolus 0.0032 0.0036 0.0059 0.0025 0.0027 0.0061 0.0105 0.0045 0.0026 0.003 0.0047 0.007 0.0035 0.0015 0.0019 0.0025 0.0046 0.0024
Nucleus 0.1155 0.2135 0.312 0.8873 0.8636 0.4131 0.1461 0.3773 0.45 0.9082 0.8826 0.4178 0.0913 0.2776 0.3675 0.8791 0.8583 0.3032
Peroxisomes 0.0029 0.0002 0.0091 0.0001 0.0011 0.0006 0.0083 0.0003 0.0048 0.0004 0.0038 0.0009 0.0081 0 0.0008 0.0208 0.0029 0.0001
Punctate Nuclear 0.0087 0.0069 0.0315 0.0029 0.0079 0.0262 0.0088 0.0106 0.0133 0.0198 0.0137 0.0171 0.0088 0.0019 0.007 0.0114 0.015 0.0058
Vacuole 0.0053 0.0086 0.0031 0.0029 0.0005 0.0019 0.012 0.0038 0.0022 0.0008 0.0058 0.0035 0.0062 0.0013 0.0018 0.0002 0.0007 0.0012
Vacuole Periphery 0.0011 0.0004 0.0012 0.002 0.0009 0.0007 0.0019 0.0006 0.0005 0.0002 0.0028 0.0007 0.0013 0.0002 0.0004 0.0001 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.4552 24.5649 85.5325 80.1405 27.4064 38.0274 47.2869 86.3975 72.6965 31.3541
Translational Efficiency 3.2406 3.5422 2.3808 1.7762 2.1026 0.9954 2.4094 2.4171 2.4692 1.9989

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1794 993 280 144 1837 1911 259 1192 3631 2904 539 1336

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 691.28 752.09 1001.52 1280.74 696.88 783.08 937.64 1154.37 694.11 772.48 970.82 1167.99
Standard Deviation 81.92 120.48 132.82 207.18 80.92 103.73 150.03 280.29 81.46 110.73 144.91 276.15
Intensity Change Log 2 — 0.121635 0.534849 0.889636 — 0.168249 0.428124 0.728124 — 0.145225 0.482258 0.810813

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000089 0.000642 0.000942 0.000904 0.000074 0.000254 0.000814 0.000601 0.000081 0.000387 0.000880 0.000634
Bud Neck 0.008641 0.022797 0.008812 0.011627 0.005654 0.025318 0.004247 0.017642 0.007130 0.024456 0.006618 0.016993
Bud Site 0.002810 0.013808 0.002415 0.001181 0.002536 0.014097 0.001487 0.002614 0.002671 0.013998 0.001969 0.002460
Cell Periphery 0.000538 0.000477 0.000320 0.000072 0.000333 0.000570 0.000237 0.000239 0.000434 0.000538 0.000280 0.000221
Cytoplasm 0.302262* 0.319149* 0.067359 0.001875 0.427108* 0.314089* 0.025967 0.009337 0.365425* 0.315819* 0.047469 0.008533
Cytoplasmic Foci 0.077998 0.086080 0.009807 0.000032 0.072186 0.042889 0.002743 0.000321 0.075058 0.057658 0.006412 0.000290
Eisosomes 0.000074 0.000066 0.000094 0.000031 0.000059 0.000046 0.000045 0.000032 0.000066 0.000053 0.000071 0.000032
Endoplasmic Reticulum 0.001315 0.003231 0.008804 0.004318 0.000746 0.001569 0.005621 0.002903 0.001027 0.002137 0.007275 0.003056
Endosome 0.003492 0.009189 0.002792 0.000171 0.002465 0.005172 0.000562 0.000669 0.002972 0.006546 0.001720 0.000615
Golgi 0.000569 0.001692 0.000135 0.000064 0.000830 0.001244 0.000072 0.000467 0.000701 0.001397 0.000105 0.000423
Lipid Particles 0.005432 0.003037 0.002669 0.000101 0.003743 0.002524 0.000663 0.000118 0.004578 0.002699 0.001705 0.000116
Mitochondria 0.002502 0.003549 0.000984 0.001630 0.004216 0.005910 0.000740 0.001977 0.003369 0.005103 0.000867 0.001939
Mitotic Spindle 0.001419 0.002561 0.001753 0.000452 0.004216 0.007822 0.000476 0.002639 0.002834 0.006023 0.001139 0.002403
None 0.022217 0.016725 0.005450 0.001781 0.015637 0.018251 0.007831 0.005559 0.018888 0.017729 0.006594 0.005152
Nuclear Periphery 0.000868 0.000670 0.001290 0.000539 0.000529 0.000804 0.000400 0.000968 0.000696 0.000758 0.000863 0.000922
Nuclear Periphery Foci 0.000466 0.000896 0.001782 0.000091 0.000284 0.000278 0.000478 0.000936 0.000374 0.000489 0.001156 0.000845
Nucleolus 0.003992 0.005289 0.004414 0.004321 0.002222 0.004696 0.004952 0.005023 0.003096 0.004899 0.004673 0.004948
Nucleus 0.549923* 0.471581* 0.846283* 0.965508* 0.444477* 0.532639* 0.929999* 0.941741* 0.496576* 0.511761* 0.886511* 0.944303*
Peroxisomes 0.002667 0.006985 0.000435 0.000197 0.001807 0.003788 0.000242 0.000111 0.002232 0.004881 0.000343 0.000120
Vacuole 0.012156 0.030280 0.033213 0.004994 0.010475 0.017172 0.012284 0.004010 0.011306 0.021654 0.023156 0.004116
Vacuole Periphery 0.000569 0.001295 0.000248 0.000109 0.000404 0.000868 0.000138 0.002093 0.000485 0.001014 0.000195 0.001879

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.33 -12.13 -10.56 -3.30 -0.59 -3.50 -11.15 -15.54 -6.79 2.52 -4.61 -16.22 -18.23 -4.73 4.57
Bud Neck -5.93 -1.54 -3.20 2.73 -1.61 -11.13 1.11 -10.42 2.04 -11.42 -12.18 -1.01 -10.13 2.27 -8.48
Bud Site -7.17 -1.17 1.21 7.52 2.34 -7.59 1.35 -0.62 7.19 -2.05 -10.15 0.28 -0.44 9.29 -0.67
Cell Periphery 0.85 2.83 6.83 5.92 3.90 -3.14 1.71 1.56 4.27 0.11 -1.76 3.21 3.76 5.00 1.19
Cytoplasm -1.29 22.28 43.50 32.74 7.55 11.47 42.72 56.30 43.46 3.62 6.71 43.86 67.01 53.19 7.39
Cytoplasmic Foci -1.30 17.68 21.97 17.47 5.10 7.59 22.29 24.35 17.86 2.48 5.24 27.52 32.42 24.59 5.51
Eisosomes 1.05 -3.35 4.69 4.28 6.73 3.83 1.63 6.53 3.93 2.94 3.82 -2.20 8.25 6.02 7.80
Endoplasmic Reticulum -6.78 -10.06 -5.10 -3.24 3.55 -3.70 -10.03 -13.25 -5.89 5.58 -6.25 -13.14 -12.32 -5.52 8.91
Endosome -5.11 -0.28 6.42 8.45 6.32 -4.24 7.79 6.80 7.08 0.32 -6.09 1.96 7.90 10.58 5.12
Golgi -2.90 4.65 5.19 4.21 2.26 -1.06 4.51 1.52 2.02 -1.56 -2.48 6.10 1.48 3.10 -1.35
Lipid Particles 3.54 3.21 9.13 8.51 6.39 2.65 7.17 9.40 9.39 3.58 4.69 6.16 13.00 12.66 7.29
Mitochondria -1.67 3.37 1.33 3.33 -4.59 -1.43 3.27 1.87 5.58 -6.30 -2.43 4.24 2.01 6.06 -5.95
Mitotic Spindle -1.85 -1.11 -0.43 1.11 0.84 -2.23 3.11 0.23 2.30 -2.40 -3.16 0.83 -0.57 1.83 -1.14
None 2.25 9.91 11.70 7.29 2.21 -1.44 3.72 6.81 7.47 0.96 0.80 9.24 10.38 8.77 0.66
Nuclear Periphery 1.28 -4.68 -0.91 -2.11 3.12 -2.22 0.69 -2.30 -1.12 -2.58 -0.67 -4.12 -1.77 -1.47 0.16
Nuclear Periphery Foci -2.12 -2.75 1.14 2.92 3.01 -0.08 -2.47 -1.79 -1.77 -0.84 -1.50 -3.21 -1.55 -1.20 1.05
Nucleolus -1.85 0.31 -1.22 -0.79 -1.28 -5.26 -2.71 -4.10 -1.49 -1.57 -4.52 -2.02 -3.53 -1.57 -2.30
Nucleus 5.51 -16.50 -32.82 -32.80 -7.49 -7.65 -37.19 -52.61* -42.67 -1.70 -1.67 -34.57 -60.38 -53.32 -7.03
Peroxisomes -4.41 5.71 6.29 7.55 4.32 -3.48 4.54 4.85 7.64 3.02 -5.24 7.25 8.09 10.78 7.11
Vacuole -7.59 -5.89 -0.72 2.53 4.33 -5.46 -2.28 2.73 6.24 2.99 -8.95 -6.16 2.65 8.43 6.73
Vacuole Periphery -3.58 1.89 2.70 8.20 3.89 -4.63 3.76 -1.98 -1.35 -2.34 -5.00 3.26 -1.81 -1.02 -2.23
Standard name
Human Ortholog
Description Nucleolar protein of unknown function; positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis; SPO12 has a paralog, BNS1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (32%), cytoplasm (20%), mixed (38%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (2.8e-08)
nucleus - MA (3e-18)
Subcompartmental Group N/A

Spo12

Spo12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spo12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available