Standard name
Human Ortholog
Description 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.17 0.36 0 0 0.1 0.06 0.08 0.08 0.11 0.14 0.07 0 0.41 0.52 0.6 0.25 0.58 0.48 0.12 0.13 0 0.09 0.06 0.09
Cytoplasm 0.9 0.68 0.97 0.98 1.0 0.96 0.93 0.96 0.96 0.9 0.93 0.97 0.6 0.54 0.46 0.85 0.5 0.72 0.83 0.82 0.86 0.87 0.91 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 22 40 1 2 5 3 8 10 13 15 6 5 28 44 100 40 77 31 27 28 0 12 8 17
Cytoplasm 119 75 34 47 50 45 92 119 110 94 80 111 41 46 78 133 66 46 185 179 56 126 136 160
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 4 6 1 2 2 0 1 0 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 1 1 0 1 0 0 0 0
Unique Cell Count 132 111 35 48 50 47 99 124 115 104 86 114 68 85 168 157 133 64 223 219 66 145 150 190
Labelled Cell Count 143 116 36 50 55 48 102 129 123 110 88 116 69 90 182 178 150 79 223 219 66 145 150 190


Ambiguous, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 536.9 612.0 348.9 399.9 431.5 363.8 353.1 346.3 326.4 336.2 288.3 299.4 659.8 640.8 631.8 565.8 615.5 602.3 504.4 509.5 505.7
Std Deviation (1e-4) 101.5 116.5 177.9 132.8 100.5 116.7 114.2 121.9 108.5 95.2 104.8 88.8 113.1 153.3 142.0 156.6 163.5 166.5 154.3 152.8 177.9
Intensity Change (Log2) – – – 0.2 0.31 0.06 0.02 -0.01 -0.1 -0.05 -0.28 -0.22 0.92 0.88 0.86 0.7 0.82 0.79 0.53 0.55 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 4.1 5.0 6.1 3.0 5.8 4.6
Cytoplasm 0.2 1.2 -0.3 -0.9 -0.3 -0.4 -1.3 -0.9 0.1 -4.0 -4.5 -5.5 -2.0 -5.1 -3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0153 0.0001 0.017 0.0002 0.0094 0.0003 0.0012 0.0001 0.0061 0.0069 0.0056 0.0027 – – – – – –
Bud 0.0196 0 0.0059 0.0001 0.0173 0.0002 0.0048 0.0001 0.0139 0.0018 0.0021 0.0016 – – – – – –
Bud Neck 0.0018 0 0.0009 0.0001 0.0145 0.0004 0.0004 0 0.001 0.0005 0.0008 0.0004 – – – – – –
Bud Periphery 0.0094 0 0.003 0 0.012 0 0.0025 0 0.0043 0.0031 0.0009 0.0004 – – – – – –
Bud Site 0.0083 0 0.0015 0.0001 0.047 0 0.003 0 0.0042 0.0019 0.0015 0.0004 – – – – – –
Cell Periphery 0.0334 0 0.0209 0.0008 0.1518 0 0.0071 0 0.0035 0.0216 0.0077 0.0015 – – – – – –
Cytoplasm 0.2856 0.9778 0.3595 0.8602 0.4209 0.9098 0.2584 0.9799 0.6004 0.3778 0.4739 0.7982 – – – – – –
Cytoplasmic Foci 0.0455 0 0.0673 0.0079 0.006 0.0001 0.0559 0.0029 0.0432 0.0254 0.0358 0.0047 – – – – – –
Eisosomes 0.0043 0 0.0037 0.0016 0.0081 0 0.0028 0 0.0012 0.0216 0.0237 0.0006 – – – – – –
Endoplasmic Reticulum 0.0099 0 0.003 0 0.0247 0.0001 0.0031 0 0.002 0.0007 0.0019 0.0003 – – – – – –
Endosome 0.0204 0 0.0042 0 0.0052 0 0.0044 0 0.0111 0.0008 0.0033 0.0003 – – – – – –
Golgi 0.0018 0 0.0113 0 0.0021 0 0.0004 0 0.0015 0.0011 0.001 0.0002 – – – – – –
Lipid Particles 0.005 0 0.0181 0.002 0.0036 0 0.0053 0 0.0071 0.0071 0.0099 0.0009 – – – – – –
Mitochondria 0.1001 0.0002 0.1515 0.0184 0.0504 0.0014 0.0963 0.0006 0.0411 0.196 0.191 0.0099 – – – – – –
None 0.0409 0.0002 0.0976 0.0037 0.0417 0.0009 0.0332 0.0031 0.0501 0.154 0.0501 0.0076 – – – – – –
Nuclear Periphery 0.0497 0.0013 0.0026 0.0012 0.0156 0.0079 0.0556 0.001 0.0384 0.0042 0.0683 0.0497 – – – – – –
Nucleolus 0.1693 0 0.162 0.0846 0.01 0.0003 0.2362 0.0007 0.0568 0.0576 0.0303 0.0167 – – – – – –
Nucleus 0.1304 0.0201 0.038 0.0154 0.0389 0.0777 0.1305 0.0101 0.0682 0.017 0.0581 0.0906 – – – – – –
Peroxisomes 0.0034 0 0.0063 0.0008 0.001 0 0.0024 0 0.0025 0.0032 0.0034 0.0005 – – – – – –
Punctate Nuclear 0.0041 0 0.0019 0.0002 0.0015 0.0002 0.0194 0.0013 0.0178 0.0021 0.0023 0.0025 – – – – – –
Vacuole 0.0265 0 0.0194 0.0009 0.1149 0.0002 0.0529 0 0.0152 0.0214 0.0163 0.0035 – – – – – –
Vacuole Periphery 0.0153 0 0.0044 0.0016 0.0033 0.0003 0.0241 0.0001 0.0103 0.0743 0.0122 0.0068 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 656.0377 686.4373 628.8655 528.9018 532.83 759.2021 767.8377 674.9581 752.6892 571.62
Translational Efficiency 1.665 1.6205 1.4971 2.0861 1.7736 1.5291 1.7303 1.875 1.6756 1.7745

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1379 1582 2032 1090 2452 1960 553 294 3831 3542 2585 1384

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 26851.88 34638.64 30084.02 28876.46 33059.70 39121.75 33271.12 41675.38 30825.14 37119.41 30765.83 31595.31
Standard Deviation 6123.18 8834.02 6098.94 6678.04 6701.00 8564.24 6292.36 8764.27 7149.45 8967.16 6278.37 8879.52
Intensity Change Log 2 — 0.367359 0.163974 0.104871 — 0.242897 0.009197 0.334122 — 0.300011 0.080627 0.235849

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000041 0.000048 0.000042 0.000173 0.000023 0.000091 0.000086 0.000031 0.000030 0.000072 0.000051 0.000143
Bud Neck 0.000255 0.000209 0.000389 0.000992 0.000163 0.000369 0.001113 0.002947 0.000197 0.000297 0.000544 0.001408
Bud Site 0.000981 0.000731 0.000925 0.005060 0.000549 0.001722 0.001593 0.010553 0.000705 0.001279 0.001068 0.006227
Cell Periphery 0.008487 0.025436 0.007042 0.010001 0.003442 0.030602 0.013684 0.002138 0.005258 0.028295 0.008463 0.008331
Cytoplasm 0.893257* 0.801339* 0.897585* 0.820739* 0.916121* 0.791392* 0.794159* 0.759862* 0.907891* 0.795835* 0.875459* 0.807807*
Cytoplasmic Foci 0.004478 0.000934 0.001883 0.004257 0.001923 0.001395 0.009370 0.001148 0.002843 0.001189 0.003485 0.003596
Eisosomes 0.000001 0.000001 0.000001 0.000003 0.000000 0.000002 0.000003 0.000002 0.000001 0.000002 0.000001 0.000003
Endoplasmic Reticulum 0.000035 0.000085 0.000027 0.000028 0.000126 0.000120 0.000027 0.000019 0.000093 0.000104 0.000027 0.000026
Endosome 0.000041 0.000024 0.000029 0.000175 0.000057 0.000043 0.000069 0.000390 0.000051 0.000034 0.000038 0.000221
Golgi 0.005774 0.001315 0.001338 0.001452 0.003727 0.001923 0.002649 0.000223 0.004464 0.001652 0.001618 0.001191
Lipid Particles 0.002231 0.002379 0.003155 0.006095 0.001968 0.006297 0.007061 0.000265 0.002063 0.004547 0.003991 0.004856
Mitochondria 0.000096 0.000093 0.000067 0.000180 0.000108 0.000197 0.000104 0.000100 0.000103 0.000151 0.000075 0.000163
Mitotic Spindle 0.000157 0.000022 0.000081 0.000169 0.000219 0.000085 0.000177 0.000007 0.000197 0.000057 0.000102 0.000135
None 0.011098 0.006132 0.004452 0.007762 0.007223 0.005464 0.005870 0.001500 0.008618 0.005762 0.004756 0.006432
Nuclear Periphery 0.000283 0.000132 0.000201 0.000169 0.000187 0.000240 0.000474 0.000009 0.000222 0.000192 0.000259 0.000135
Nuclear Periphery Foci 0.002418 0.000966 0.001288 0.001126 0.002094 0.001954 0.002198 0.000112 0.002211 0.001513 0.001482 0.000911
Nucleolus 0.000050 0.000077 0.000045 0.000118 0.000041 0.000083 0.000111 0.000317 0.000044 0.000080 0.000059 0.000160
Nucleus 0.046443 0.026474 0.047581 0.064079 0.036110 0.023156 0.106057 0.007467 0.039830 0.024638 0.060091 0.052053
Peroxisomes 0.000003 0.000022 0.000008 0.000039 0.000006 0.000025 0.000011 0.000290 0.000005 0.000024 0.000008 0.000092
Vacuole 0.018511 0.131205 0.032533 0.076559 0.019809 0.132030 0.053067 0.210089* 0.019342 0.131661 0.036926 0.104925
Vacuole Periphery 0.005360 0.002375 0.001329 0.000823 0.006102 0.002809 0.002118 0.002530 0.005835 0.002615 0.001498 0.001186

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.90 -1.27 -4.79 -4.62 -4.49 -6.72 -8.27 -1.25 5.94 7.33 -6.60 -6.36 -5.29 -3.10 -3.72
Bud Neck 0.53 -3.22 -12.51 -13.52 -7.36 -3.46 -7.99 -4.13 -3.70 -1.73 -2.76 -6.62 -10.77 -9.84 -6.03
Bud Site 0.82 -0.18 -3.38 -4.23 -3.62 -2.76 -4.63 -4.75 -3.96 -4.12 -1.83 -2.23 -5.76 -4.99 -5.12
Cell Periphery -9.02 1.25 -0.46 8.60 -1.68 -16.06 -6.66 1.34 14.37 6.29 -18.56 -4.97 -3.02 14.96 1.18
Cytoplasm 10.99 1.29 14.03 2.47 13.96 18.49 16.09 9.12 1.20 -0.91 21.52 11.29 19.41 2.31 10.93
Cytoplasmic Foci 7.60 4.24 -0.57 -4.40 -2.81 1.05 -7.46 3.45 2.04 8.22 5.83 -3.98 -1.77 -3.89 0.38
Eisosomes -1.92 -1.82 -8.48 -6.80 -7.64 -8.45 -8.32 -6.18 3.04 4.82 -8.41 -8.50 -10.56 -4.45 -5.60
Endoplasmic Reticulum -3.76 1.54 1.85 4.58 0.62 0.26 3.95 4.35 7.00 1.88 -0.49 4.08 4.32 7.91 1.08
Endosome 3.76 1.15 -1.21 -1.44 -1.29 1.55 -1.26 -4.78 -4.98 -4.48 3.15 1.56 -2.10 -2.37 -2.24
Golgi 9.23 9.13 6.45 0.33 0.49 4.41 2.56 9.31 10.26 8.49 9.01 9.12 6.77 1.61 1.62
Lipid Particles -0.24 -3.36 -6.18 -6.08 -4.15 -7.57 -5.46 5.11 12.13 7.32 -6.73 -6.08 -5.70 -1.54 -1.49
Mitochondria 0.42 1.83 -1.37 -1.50 -1.84 -2.32 -0.26 0.97 2.59 1.03 -2.00 2.91 -1.24 -0.14 -1.75
Mitotic Spindle 5.35 1.35 0.29 -2.11 -0.54 2.27 0.50 4.16 2.63 2.61 3.62 1.88 1.75 -1.05 0.27
None 3.14 4.82 2.77 -0.01 -1.36 1.81 2.01 4.66 3.21 2.60 3.41 5.76 3.02 0.58 -0.57
Nuclear Periphery 5.31 1.16 1.99 -4.78 0.81 -1.75 -7.54 9.11 15.66 12.66 1.73 -3.50 2.51 1.09 6.22
Nuclear Periphery Foci 6.18 3.60 4.27 -3.54 0.61 0.62 -0.75 7.00 7.69 8.36 3.71 2.77 5.94 2.98 3.95
Nucleolus -2.57 0.53 -5.27 -4.31 -6.75 -3.44 -2.79 -9.85 -7.64 -5.65 -4.52 -2.09 -10.23 -6.61 -8.67
Nucleus 7.93 -2.01 -7.80 -14.63 -6.59 5.49 -13.98 15.91 10.48 19.21 9.03 -11.29 -7.34 -12.98 1.78
Peroxisomes -5.19 -2.99 -3.72 -1.38 -3.07 -6.50 -1.78 -4.21 -3.92 -4.10 -8.15 -2.72 -5.22 -4.02 -4.94
Vacuole -18.90 -6.55 -15.76 3.77 -11.87 -22.39 -8.63 -12.00 -4.43 -8.22 -29.22 -10.34 -19.61 1.24 -14.10
Vacuole Periphery 3.84 5.27 6.10 5.55 4.48 4.84 5.56 4.52 0.59 -0.62 6.24 8.54 9.30 6.47 2.33
Standard name
Human Ortholog
Description 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (93%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Gnd1

Gnd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gnd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available