Standard name
Human Ortholog
Description Subunit of TORC1; TORC1 is a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.46 0.65 0.77 0.68 0.71 0.65 0.6 0.66 0.6 – 0.41 0.41 0.45 0.55 0.68 0.61 0.65 0.54 0.05 0.1 0.09 0.09 0.1 0
Endoplasmic Reticulum 0.07 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0.18 0 0 0 0.06 0 0 0.06 0 – 0 0 0 0.06 0 0.15 0.09 0.21 0.19 0.08 0.1 0.15 0.14 0.22
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.09 0.31 0.39 0.23 0.43 0.43 0.27 0.61 – 0.66 0.65 0.12 0 0.18 0.08 0 0 0.05 0.05 0.17 0.05 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.4 0.36 0.1 0.1 0.23 0.16 0.15 0.24 0.09 – 0.11 0.08 0.51 0.43 0.25 0.34 0.31 0.39 0.61 0.71 0.57 0.65 0.66 0.59
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 1 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 1 0 0 1 0 0 0
Bud 1 1 7 1 3 0 3 0 2 – 0 0 0 2 9 1 0 1 0 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 0 0 1 0 0 – 2 2 0 1 0 1 1 0 0 0 0 0 0 0
Cytoplasm 91 154 104 71 145 81 124 153 74 – 29 29 53 155 193 137 111 100 12 26 13 19 21 8
Endoplasmic Reticulum 13 5 5 0 0 0 1 1 3 – 1 0 5 12 4 8 9 8 7 2 3 2 0 5
Endosome 35 5 1 2 13 4 6 14 0 – 0 0 4 17 1 33 15 39 51 22 15 31 31 65
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 5 2 3 0 0 0 2
Mitochondria 9 21 42 41 47 53 88 63 76 – 46 46 14 7 52 18 5 7 13 13 26 10 6 16
Nucleus 1 2 0 1 0 3 2 1 0 – 1 1 0 1 0 2 2 0 0 0 0 2 1 2
Nuclear Periphery 4 6 0 0 0 0 1 0 1 – 1 2 0 2 2 0 0 0 12 2 2 1 1 11
Nucleolus 2 0 0 1 1 0 1 3 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 1 0 0 0 1 – 0 0 0 0 0 1 2 0 0 0 0 0 1 1
Vac/Vac Membrane 79 86 14 11 46 20 31 55 11 – 8 6 61 123 72 77 53 73 161 185 90 141 148 174
Unique Cell Count 198 238 135 105 203 124 207 231 124 70 71 119 283 284 225 172 186 266 262 158 217 224 296
Labelled Cell Count 240 282 173 128 257 161 258 290 168 88 86 137 321 333 279 200 234 266 262 158 217 224 296


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 4.9 4.0 4.2 4.6 4.2 4.1 4.7 4.3 – 4.2 4.2 5.2 5.5 4.6 6.2 6.8 7.5 5.6 5.1 5.6
Std Deviation (1e-4) 0.8 1.0 0.8 1.8 1.6 1.5 1.5 2.0 2.0 – 1.8 1.8 1.1 1.0 0.8 1.2 1.6 2.4 1.4 1.2 1.3
Intensity Change (Log2) – – – 0.07 0.21 0.07 0.06 0.22 0.09 – 0.08 0.08 0.39 0.46 0.2 0.64 0.76 0.91 0.49 0.35 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -1.1 -2.1 -3.3 -2.2 -3.0 – -5.1 -5.2 -5.3 -4.4 -1.9 -3.2 -2.4 -4.3
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0.3 0 0 0.6 0.3
Endosome 0 0 0 0 0 0 – 0 0 0 2.5 0 4.4 3.1 5.4
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 -3.7 -8.5 0 -5.7 -6.8 -6.7
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 2.9 1.4 1.2 3.2 -0.4 – 0.2 -0.4 7.1 6.7 3.5 5.0 4.3 5.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0408 0.0048 0.0208 0.0026 0.0001 0.0022 0.0016 0.0013 0.0121 0.0027 0 0 – – – – – –
Bud 0.0013 0.0011 0.0026 0.0028 0.0001 0.0003 0.0022 0.0074 0.0009 0.0002 0.0001 0.0004 – – – – – –
Bud Neck 0.0033 0.0007 0.0046 0.0343 0.0001 0.0032 0.0004 0.0016 0.0006 0.0003 0.0001 0.0003 – – – – – –
Bud Periphery 0.0023 0.0014 0.0036 0.0046 0.0001 0.0007 0.0031 0.0024 0.0007 0.0001 0.0001 0.0005 – – – – – –
Bud Site 0.0083 0.0099 0.0103 0.0098 0.0003 0.0006 0.0016 0.005 0.003 0.0005 0.0001 0.0005 – – – – – –
Cell Periphery 0.0025 0.0006 0.005 0.0019 0.0001 0.0009 0.0005 0.0007 0.0002 0.0001 0 0.0001 – – – – – –
Cytoplasm 0.0654 0.1466 0.1067 0.0719 0.0205 0.074 0.0673 0.1628 0.0706 0.0903 0.0044 0.0433 – – – – – –
Cytoplasmic Foci 0.0509 0.0515 0.0682 0.0472 0.0289 0.0518 0.0666 0.0798 0.1146 0.089 0.1371 0.0449 – – – – – –
Eisosomes 0.0012 0.0001 0.0019 0 0 0.0001 0.0006 0.0001 0 0 0 0 – – – – – –
Endoplasmic Reticulum 0.0104 0.0064 0.0162 0.0028 0.002 0.0042 0.0051 0.0077 0.0043 0.0023 0.0013 0.0067 – – – – – –
Endosome 0.4599 0.47 0.4628 0.4358 0.6248 0.56 0.4249 0.369 0.4724 0.4262 0.7229 0.5247 – – – – – –
Golgi 0.0258 0.0186 0.0202 0.0457 0.0217 0.0162 0.0114 0.0058 0.0382 0.0278 0.0117 0.013 – – – – – –
Lipid Particles 0.0327 0.0059 0.0257 0.0188 0.0028 0.0051 0.0383 0.0213 0.0105 0.0145 0.0086 0.0208 – – – – – –
Mitochondria 0.0402 0.0232 0.025 0.0177 0.0058 0.0169 0.0027 0.0096 0.0114 0.0043 0.0007 0.0024 – – – – – –
None 0.0201 0.0313 0.0251 0.0007 0.0558 0.0088 0.128 0.0372 0.0165 0.0052 0.0002 0.0033 – – – – – –
Nuclear Periphery 0.0141 0.0045 0.0068 0.002 0.0045 0.0038 0.0108 0.0115 0.0044 0.0028 0.0012 0.023 – – – – – –
Nucleolus 0.0022 0.0006 0.0009 0.0008 0.0001 0.0007 0.0019 0.0037 0.0012 0.0002 0.0001 0.0004 – – – – – –
Nucleus 0.015 0.0027 0.0027 0.0025 0.0005 0.003 0.0086 0.0031 0.0016 0.0011 0.0003 0.0024 – – – – – –
Peroxisomes 0.0148 0.0013 0.0104 0.0026 0.003 0.0038 0.0077 0.0131 0.0084 0.039 0.001 0.0024 – – – – – –
Punctate Nuclear 0.0012 0.0006 0.0006 0.0004 0.0002 0.0003 0.0159 0.0022 0.022 0.001 0.0002 0.0013 – – – – – –
Vacuole 0.1523 0.1812 0.1568 0.2412 0.1581 0.1607 0.1771 0.2126 0.152 0.2169 0.0869 0.2231 – – – – – –
Vacuole Periphery 0.0354 0.0372 0.0233 0.0538 0.0707 0.0826 0.0239 0.0422 0.0543 0.0755 0.023 0.0864 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.171 17.9379 18.1652 22.4392 18.5178 17.9316 24.0583 23.9065 22.2141 25.0891
Translational Efficiency 0.5011 0.4364 0.4161 0.3148 0.3176 0.4021 0.417 0.2861 0.3322 0.3565

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
111 1436 729 799 1524 1779 1223 51 1635 3215 1952 850

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 621.92 725.89 839.23 743.03 618.33 707.75 761.75 749.34 618.57 715.85 790.69 743.41
Standard Deviation 93.84 98.36 104.69 122.72 72.34 97.54 92.03 74.99 74.00 98.32 103.94 120.40
Intensity Change Log 2 — 0.223022 0.432337 0.256691 — 0.194863 0.300941 0.277243 — 0.209052 0.368324 0.266974

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000213 0.000104 0.000521 0.000867 0.000090 0.000129 0.000214 0.001257 0.000099 0.000118 0.000329 0.000891
Bud Neck 0.043135 0.012435 0.010189 0.016381 0.012732 0.011573 0.014357 0.008758 0.014796 0.011958 0.012800 0.015923
Bud Site 0.009167 0.014370 0.022196 0.037599 0.010598 0.014886 0.010747 0.028065 0.010501 0.014656 0.015023 0.037027
Cell Periphery 0.000485 0.000116 0.000077 0.000133 0.000136 0.000240 0.000134 0.000330 0.000160 0.000185 0.000112 0.000145
Cytoplasm 0.207425* 0.189572* 0.182816* 0.253976* 0.217728* 0.104360* 0.299297* 0.196302* 0.217029* 0.142420* 0.255796* 0.250516*
Cytoplasmic Foci 0.280659* 0.253090* 0.164656 0.111208 0.273173* 0.187333* 0.116851 0.108181 0.273682* 0.216704* 0.134704 0.111027
Eisosomes 0.000800 0.000057 0.000040 0.000068 0.000100 0.000061 0.000071 0.000359 0.000148 0.000059 0.000059 0.000085
Endoplasmic Reticulum 0.003511 0.003710 0.004018 0.001070 0.009783 0.004495 0.001904 0.002856 0.009357 0.004144 0.002693 0.001177
Endosome 0.113033 0.166591 0.139394 0.073376 0.112734 0.214701* 0.082871 0.094488 0.112755 0.193212 0.103980 0.074643
Golgi 0.035300 0.011855 0.009708 0.006242 0.013049 0.021948 0.001501 0.012688 0.014560 0.017440 0.004566 0.006629
Lipid Particles 0.032949 0.011367 0.004860 0.004224 0.019835 0.011357 0.006299 0.017560 0.020726 0.011361 0.005761 0.005024
Mitochondria 0.074480 0.017121 0.007217 0.013312 0.024419 0.032541 0.002353 0.001340 0.027818 0.025654 0.004170 0.012593
Mitotic Spindle 0.000192 0.002503 0.047341 0.030173 0.001344 0.005885 0.008819 0.000143 0.001266 0.004374 0.023206 0.028371
None 0.000320 0.001654 0.000643 0.000981 0.002232 0.000580 0.001810 0.000651 0.002102 0.001060 0.001374 0.000961
Nuclear Periphery 0.000365 0.000694 0.002842 0.000658 0.001086 0.000919 0.001337 0.001344 0.001037 0.000819 0.001899 0.000699
Nuclear Periphery Foci 0.000123 0.000603 0.006520 0.000853 0.000592 0.000471 0.002607 0.000473 0.000560 0.000530 0.004068 0.000830
Nucleolus 0.002465 0.001952 0.004696 0.000845 0.001819 0.001127 0.004762 0.000777 0.001863 0.001496 0.004738 0.000841
Nucleus 0.051327 0.013641 0.045372 0.023001 0.023123 0.007638 0.118635 0.151802* 0.025038 0.010319 0.091274 0.030730
Peroxisomes 0.012595 0.003257 0.008258 0.005331 0.002917 0.004352 0.004065 0.024320 0.003574 0.003863 0.005631 0.006470
Vacuole 0.108758 0.240617* 0.298103* 0.413068* 0.227064* 0.287333* 0.316606* 0.344054* 0.219032* 0.266467* 0.309696* 0.408928*
Vacuole Periphery 0.022700 0.054691 0.040535 0.006634 0.045444 0.088071 0.004760 0.004253 0.043900 0.073162 0.018121 0.006491

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.28 -3.31 -2.84 -3.78 -1.13 -1.22 -1.66 -1.42 -1.37 -1.27 -0.93 -4.67 -4.06 -3.93 -2.55
Bud Neck 3.42 3.58 3.13 -1.40 -2.31 0.86 -1.04 2.05 1.53 2.68 2.17 1.23 0.15 -1.71 -0.92
Bud Site -2.09 -4.69 -5.81 -5.24 -3.04 -2.89 -0.04 -1.01 -0.77 -1.01 -3.51 -3.28 -6.33 -5.21 -5.08
Cell Periphery 4.58 5.04 4.51 -0.14 -2.59 -1.13 0.41 -3.27 -0.70 -3.36 -0.48 4.19 1.80 1.04 -1.22
Cytoplasm 0.88 0.97 -2.53 -6.22 -5.58 16.86 -8.50 0.73 -3.01 3.27 11.86 -4.92 -3.66 -11.47 -0.02
Cytoplasmic Foci 1.57 6.81 9.98 22.08 7.63 13.35 24.64 9.47 4.66 0.74 9.86 24.50 26.89 21.20 5.79
Eisosomes 2.93 2.99 2.91 -1.31 -4.90 7.59 5.62 -2.82 -3.28 -3.15 4.88 4.80 3.53 -3.05 -2.84
Endoplasmic Reticulum -0.14 -0.52 1.78 9.81 9.98 4.76 7.47 3.23 0.92 -0.43 5.15 6.61 8.33 11.30 8.17
Endosome -3.80 -1.27 3.68 17.52 9.72 -18.12 5.65 0.92 5.14 -0.35 -17.47 2.50 9.70 25.63 7.10
Golgi 2.98 3.36 3.84 5.10 2.66 -5.55 11.38 0.13 1.20 -1.26 -2.20 9.22 5.97 8.64 -0.92
Lipid Particles 4.48 5.87 6.16 7.29 2.10 5.96 10.76 0.37 -0.58 -1.15 7.44 12.92 12.87 7.32 1.73
Mitochondria 4.14 4.92 4.56 2.22 -1.38 -3.22 13.07 13.04 14.64 1.01 0.98 12.94 5.87 5.65 -2.38
Mitotic Spindle -4.08 -10.51 -7.00 -6.39 2.74 -3.63 -3.90 1.78 5.42 4.76 -3.52 -10.14 -6.65 -5.88 -1.11
None -2.78 -2.73 -2.58 1.34 -1.23 3.11 0.84 2.33 -0.07 2.15 1.99 1.49 2.23 0.42 1.54
Nuclear Periphery -2.01 -5.43 -3.79 -0.41 4.66 0.54 -1.19 -0.32 -0.59 0.15 0.91 -3.49 1.19 0.19 5.81
Nuclear Periphery Foci -6.24 -8.22 -4.66 -1.79 7.23 1.12 -2.78 0.82 0.07 2.93 0.37 -6.80 -1.93 -2.22 6.04
Nucleolus 0.28 -1.27 0.90 2.33 4.53 1.60 -3.14 2.49 1.28 4.52 0.90 -3.99 2.50 2.37 6.06
Nucleus 2.57 0.08 1.87 -4.73 5.91 10.07 -19.19 -4.16 -4.69 -0.91 8.52 -17.87 -1.95 -7.64 14.21
Peroxisomes 4.19 1.82 3.42 -2.42 2.81 -2.22 -2.60 -1.14 -1.06 -1.07 -0.70 -3.88 -1.87 -1.58 -0.16
Vacuole -9.02 -12.45 -18.77 -15.78 -9.39 -7.63 -9.98 -3.21 -1.76 -1.03 -7.28 -12.55 -17.98 -14.44 -9.86
Vacuole Periphery -6.47 -2.68 4.00 18.35 10.14 -12.01 18.30 15.54 26.27* 0.36 -10.37 11.51 17.17 31.71* 7.59
Standard name
Human Ortholog
Description Subunit of TORC1; TORC1 is a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
Localization
Cell Percentages cytoplasm (30%), vacuolar membrane (18%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kog1

Kog1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kog1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available