Standard name
Human Ortholog
Description Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.95 0.97 0.96 0.94 0.82 0.8 0.77 0.74 0.75 0.69 0.99 0.99 0.99 0.98 0.94 0.98 0.93 0.98 0.86 0.94 0.95 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0 0.05 0.13 0.24 0.22 0.31 0.34 0.36 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.09 0 0.06 0 0.05 0 0 0 0 0.08 0 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Bud 0 0 2 3 1 0 0 2 9 3 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 2 1 0 2 7 7 1 6 5 2 3 2 0 1 4 5 7 0 0 0 0 0 0
Cytoplasm 164 228 148 143 215 321 304 325 231 236 117 104 268 433 417 333 147 125 155 224 137 145 324 279
Endoplasmic Reticulum 0 3 0 3 1 0 2 0 4 4 2 7 3 1 1 18 5 3 0 0 0 1 3 4
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 1 0 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Mitochondria 0 2 7 4 11 45 90 91 94 110 56 54 1 2 0 4 0 1 1 0 3 1 0 3
Nucleus 8 0 5 0 0 0 4 7 4 4 3 4 2 1 0 1 1 1 0 1 1 0 1 0
Nuclear Periphery 0 0 0 0 0 0 3 1 0 5 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 1 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 0 0 0 2 6 4 6 35 10 19 2 7 0 2 2 5 12 2 4 1 10 2 3 4
Unique Cell Count 168 232 155 148 225 343 370 406 299 320 155 151 272 438 420 339 156 128 167 229 160 155 341 308
Labelled Cell Count 177 235 163 155 236 377 416 464 358 386 183 180 276 439 421 367 171 139 167 229 160 155 341 308


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 8.5 6.0 6.2 6.3 5.7 4.9 5.5 5.2 4.8 4.8 4.7 8.9 8.5 8.1 9.6 11.4 12.4 8.7 8.9 9.7
Std Deviation (1e-4) 1.3 1.3 1.1 0.8 1.2 1.0 1.2 1.1 1.1 1.0 1.0 0.8 1.4 1.3 1.4 2.0 3.0 2.7 1.4 1.5 1.5
Intensity Change (Log2) – – – 0.05 0.07 -0.07 -0.29 -0.12 -0.2 -0.31 -0.32 -0.37 0.57 0.51 0.44 0.68 0.93 1.04 0.53 0.57 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0 -0.8 -4.0 -4.5 -5.0 -5.6 -5.0 -6.1 1.9 2.6 3.1 1.8 -0.5 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.8 0.2 2.9 5.3 5.0 6.5 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.8 0 3.1 0 0 0 0 0 0 3.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1311 5.7848 4.8644 4.6549 4.1956 5.0693 3.2908 3.9865 3.598 3.157 2.7616 3.5308 2.8961 4.7167 4.5491 3.8698 4.5323 4.552
Actin 0.0149 0.001 0.0255 0.0154 0.0318 0.0009 0.0535 0.0005 0.0074 0.0005 0.0506 0.0017 0.0318 0.0004 0.0006 0.0004 0.0007 0.0003
Bud 0.0009 0.0002 0.0082 0.0012 0.0007 0.0001 0.0001 0.0001 0.0005 0.0001 0.0003 0.0005 0.0001 0 0.0001 0.0001 0.0001 0
Bud Neck 0.0007 0.0001 0.0082 0.0009 0.0024 0.0008 0.0001 0.0001 0.0002 0.0002 0.0008 0.0005 0.0002 0.0001 0.0001 0.0003 0.0004 0.0005
Bud Periphery 0.0004 0.0001 0.0016 0.0013 0.0004 0 0.0001 0 0.0003 0 0.0002 0.0003 0.0002 0 0 0 0 0
Bud Site 0.0031 0.0006 0.0042 0.0038 0.0011 0.0001 0.0005 0.0005 0.0007 0.0001 0.0007 0 0.0006 0.0002 0.0001 0.0001 0.0001 0
Cell Periphery 0.0003 0.0002 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0003 0 0.0001 0 0 0
Cytoplasm 0.8005 0.9381 0.8721 0.9059 0.8558 0.9507 0.778 0.9383 0.8235 0.9867 0.8222 0.95 0.7294 0.9829 0.8903 0.9381 0.9723 0.9592
Cytoplasmic Foci 0.0287 0.0052 0.0091 0.0186 0.007 0.0054 0.0024 0.0014 0.0253 0.0019 0.0316 0.0034 0.0165 0.0012 0.0019 0.0022 0.0034 0.0022
Eisosomes 0.0004 0 0.0005 0.0001 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0021 0 0 0 0 0
Endoplasmic Reticulum 0.0053 0.0024 0.0034 0.0016 0.0023 0.0029 0.003 0.0026 0.0051 0.0021 0.002 0.0018 0.0065 0.0082 0.0031 0.0417 0.0114 0.0023
Endosome 0.0123 0.004 0.006 0.0113 0.0041 0.0023 0.0005 0.0004 0.0157 0.001 0.0081 0.001 0.0166 0.0007 0.001 0.0031 0.002 0.0014
Golgi 0.0038 0.0005 0.0026 0.004 0.002 0.0003 0.0012 0.0001 0.0126 0.0001 0.0188 0.0001 0.0232 0.0001 0.0001 0.0001 0.0002 0
Lipid Particles 0.003 0.0004 0.0019 0.0034 0.0012 0.0001 0.0005 0 0.0037 0 0.0131 0 0.1035 0 0 0 0.0001 0
Mitochondria 0.0044 0.0001 0.0039 0.002 0.0024 0.0002 0.0003 0 0.0023 0.0001 0.0011 0.0001 0.0102 0 0 0.0001 0.0001 0
None 0.0951 0.044 0.0397 0.0071 0.0773 0.0323 0.1555 0.0545 0.0928 0.0048 0.0047 0.038 0.0246 0.0033 0.1005 0.0091 0.0071 0.0327
Nuclear Periphery 0.0066 0.0006 0.0009 0.0005 0.0008 0.0005 0.0004 0.0004 0.001 0.0006 0.0003 0.0005 0.0025 0.0012 0.0006 0.0021 0.0007 0.0002
Nucleolus 0.0004 0 0.0006 0.0001 0 0 0 0 0.0002 0 0 0 0.0002 0 0 0 0 0
Nucleus 0.004 0.001 0.0019 0.0016 0.0021 0.0019 0.0011 0.0005 0.0009 0.0012 0.0004 0.001 0.0012 0.0011 0.0008 0.0015 0.0009 0.0007
Peroxisomes 0.0084 0.0002 0.0062 0.0188 0.0066 0.0002 0.0022 0 0.0057 0 0.0435 0.0001 0.0275 0 0 0 0 0
Punctate Nuclear 0.0056 0.0003 0.0019 0.0006 0.0005 0.0004 0.0002 0.0001 0.0012 0.0001 0.0012 0.0009 0.0014 0.0002 0.0002 0.0002 0.0003 0.0001
Vacuole 0.001 0.0006 0.0011 0.0015 0.0006 0.0007 0.0002 0.0002 0.0004 0.0004 0.0003 0.0002 0.0006 0.0002 0.0003 0.0007 0.0003 0.0001
Vacuole Periphery 0.0003 0.0001 0.0002 0.0003 0.0002 0.0001 0 0 0.0002 0.0001 0.0001 0 0.0008 0 0 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.4479 13.7257 14.3516 18.591 23.9793 20.2283 21.7632 20.6494 19.3352 18.8121
Translational Efficiency 1.9387 2.7069 1.7816 1.4429 1.2397 1.2351 1.4736 1.3363 1.3143 1.5773

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1600 1222 171 189 1763 1367 519 1444 3363 2589 690 1633

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 889.55 814.15 1249.27 1235.24 967.38 932.21 1169.30 1071.65 930.35 876.49 1189.12 1090.58
Standard Deviation 116.78 160.54 152.27 153.81 125.47 149.10 148.13 147.71 127.48 165.46 153.11 157.38
Intensity Change Log 2 — -0.127781 0.489938 0.473644 — -0.053428 0.273491 0.147679 — -0.088568 0.381235 0.313030

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000126 0.000404 0.001273 0.001380 0.000104 0.000199 0.000431 0.001536 0.000115 0.000296 0.000639 0.001518
Bud Neck 0.001270 0.002233 0.004284 0.002059 0.001183 0.001873 0.003235 0.007135 0.001224 0.002043 0.003495 0.006547
Bud Site 0.004227 0.007377 0.008615 0.021594 0.003345 0.010208 0.012359 0.043273 0.003765 0.008872 0.011431 0.040764
Cell Periphery 0.000093 0.000072 0.000380 0.000123 0.000065 0.000060 0.000134 0.000104 0.000078 0.000066 0.000195 0.000106
Cytoplasm 0.815261* 0.810762* 0.472934* 0.654964* 0.802372* 0.794775* 0.571563* 0.607409* 0.808504* 0.802321* 0.547120* 0.612913*
Cytoplasmic Foci 0.070163 0.046264 0.018829 0.018109 0.077965 0.079417 0.010495 0.023979 0.074253 0.063769 0.012560 0.023300
Eisosomes 0.000018 0.000012 0.000038 0.000023 0.000013 0.000017 0.000022 0.000024 0.000016 0.000015 0.000026 0.000024
Endoplasmic Reticulum 0.001214 0.000659 0.005030 0.000697 0.001050 0.000469 0.002275 0.002831 0.001128 0.000559 0.002958 0.002584
Endosome 0.002640 0.003637 0.004184 0.001457 0.003193 0.006449 0.002137 0.010585 0.002930 0.005122 0.002644 0.009528
Golgi 0.000662 0.000958 0.000435 0.000174 0.000658 0.002218 0.000139 0.005918 0.000660 0.001623 0.000212 0.005254
Lipid Particles 0.000874 0.000585 0.002348 0.001201 0.000871 0.001472 0.001089 0.000932 0.000872 0.001053 0.001401 0.000963
Mitochondria 0.000171 0.000348 0.000441 0.000190 0.000221 0.001702 0.000311 0.004561 0.000197 0.001063 0.000343 0.004055
Mitotic Spindle 0.000194 0.002475 0.003665 0.031519 0.001005 0.003491 0.005784 0.065516 0.000619 0.003011 0.005259 0.061582
None 0.005959 0.014003 0.003095 0.010699 0.003508 0.003573 0.007514 0.005586 0.004674 0.008496 0.006419 0.006178
Nuclear Periphery 0.000099 0.000158 0.001444 0.000406 0.000127 0.000086 0.001060 0.000838 0.000113 0.000120 0.001155 0.000788
Nuclear Periphery Foci 0.001780 0.001805 0.010446 0.003528 0.001788 0.002293 0.005758 0.002206 0.001784 0.002063 0.006919 0.002359
Nucleolus 0.000285 0.000793 0.000210 0.000138 0.000174 0.000707 0.000169 0.000288 0.000227 0.000747 0.000179 0.000271
Nucleus 0.064071 0.058748 0.328398* 0.105310 0.062413 0.030409 0.271693* 0.049499 0.063202 0.043785 0.285746* 0.055958
Peroxisomes 0.000327 0.001005 0.000249 0.004850 0.000279 0.001816 0.000144 0.003409 0.000302 0.001433 0.000170 0.003575
Vacuole 0.030228 0.046937 0.133431 0.141498 0.039186 0.057927 0.103567 0.160138 0.034924 0.052739 0.110968 0.157981
Vacuole Periphery 0.000337 0.000767 0.000274 0.000083 0.000482 0.000839 0.000121 0.004233 0.000413 0.000805 0.000159 0.003753

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.59 -11.74 -2.37 -1.35 1.90 -3.64 -10.81 -3.08 -2.33 0.07 -2.14 -14.59 -3.63 -1.68 1.86
Bud Neck -1.79 -6.70 -3.87 -2.57 4.53 -2.55 -3.37 -4.93 -3.00 -0.37 -2.78 -5.36 -4.83 -3.05 0.94
Bud Site -3.06 -5.97 -3.72 -2.43 -0.90 -5.57 -2.18 -6.54 -2.66 -3.15 -6.29 -3.09 -6.80 -3.74 -3.26
Cell Periphery 2.54 -11.42 -3.25 -4.24 8.55 1.12 -8.68 0.35 -0.60 8.41 2.80 -11.94 1.07 -1.52 11.92
Cytoplasm 1.39 24.14 20.03 19.06 -1.53 1.47 29.81 30.37 26.42 -7.42 2.04 38.00 36.83 33.06 -8.59
Cytoplasmic Foci 6.89 17.65 16.02 8.48 -0.61 -0.32 24.94 22.46 21.11 -6.43 3.93 32.35 29.87 22.94 -5.07
Eisosomes 3.81 -8.44 -4.89 -6.81 4.20 -2.75 -9.22 -8.05 -3.89 4.95 1.16 -11.44 -7.33 -7.73 7.42
Endoplasmic Reticulum 1.05 -6.05 0.44 -2.36 8.40 3.92 -7.21 0.19 -1.38 4.38 2.14 -7.23 0.36 -1.31 6.76
Endosome -1.84 -3.97 3.56 4.55 6.64 -4.74 3.73 0.45 4.32 -1.71 -4.96 0.73 0.29 3.90 -0.12
Golgi -0.71 2.17 5.25 2.33 8.92 -2.51 9.31 -1.41 1.23 -2.58 -2.57 8.03 -1.23 0.86 -2.38
Lipid Particles 1.24 -7.31 -4.19 -7.39 3.68 -1.35 -4.99 -0.24 1.30 5.15 -0.63 -6.94 -1.31 -0.06 7.79
Mitochondria -2.17 -6.68 0.03 2.20 6.84 -1.74 -2.52 -2.20 0.55 -1.93 -1.91 -5.78 -2.25 -0.00 -1.74
Mitotic Spindle -2.29 -5.03 -3.70 -3.20 -2.97 -2.17 -3.36 -7.49 -6.35 -4.25 -3.11 -4.02 -8.25 -7.18 -5.11
None -3.62 5.69 -1.24 2.34 -4.00 -0.00 -2.59 -0.70 -0.61 1.49 -3.42 0.57 0.50 3.44 0.01
Nuclear Periphery -1.91 -15.67 -9.90 -9.23 5.70 2.72 -16.89 -10.10 -10.63 6.54 -0.45 -21.80 -11.82 -11.65 8.30
Nuclear Periphery Foci -0.13 -7.68 -5.49 -5.10 5.49 -1.70 -7.23 1.36 2.57 7.77 -1.49 -10.38 -0.22 1.12 10.02
Nucleolus -4.35 0.87 1.78 6.52 2.50 -1.79 -0.92 -2.61 1.63 -1.31 -3.08 0.88 0.43 3.21 -0.72
Nucleus 1.33 -15.20 -5.47 -5.79 10.08 14.59 -21.06 -6.71 -18.51 18.78 9.36 -25.70 -8.51 -14.51 22.27
Peroxisomes -3.02 0.75 -0.94 -0.27 -0.98 -3.98 7.29 -1.82 2.13 -2.37 -4.92 5.34 -1.98 1.62 -2.48
Vacuole -6.92 -11.87 -16.00 -14.90 -5.44 -6.71 -16.97 -34.86 -30.75 -4.90 -9.63 -20.80 -39.08 -35.00 -6.43
Vacuole Periphery -2.37 0.97 4.99 4.13 10.54 -1.90 4.52 -1.57 -0.44 -2.88 -2.99 5.17 -1.68 -0.29 -2.70
Standard name
Human Ortholog
Description Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Set5

Set5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Set5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available