Standard name
Human Ortholog
Description hEST1A/B (SMG5/6)-like protein; contributes to environment-sensing adaptive gene expression responses; Esl1p and Esl2p contain a 14-3-3-like domain and a putative PilT N-terminus ribonuclease domain; ESL1 has a paralog, ESL2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.92 0.95 0.75 0.93 0.94 0.88 0.9 0.75 0.8 0.61 0.72 0.96 0.99 0.95 0.81 0.78 0.9 0.6 0.84 0.77 0.76 0.77 0.73
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0.38 0.07 0.16 0.27 0.25 0.54 0.41 0.58 0.54 0 0 0 0.06 0.08 0.05 0 0.05 0.08 0 0 0.06
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.06 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.09 0.13 0.1 0.14 0 0.05 0.11 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 2
Bud – 0 1 0 1 0 4 2 3 4 3 6 0 0 3 0 0 0 0 1 0 0 2 1
Bud Neck – 0 2 0 2 1 3 2 0 2 1 2 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site – 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery – 2 0 0 0 1 0 0 0 0 1 1 0 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm – 294 185 41 240 188 226 253 76 152 100 194 136 75 113 76 66 76 22 286 150 74 78 83
Endoplasmic Reticulum – 4 0 0 0 1 0 0 0 0 1 0 0 1 0 7 4 4 0 1 3 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0 1 1 1 1 0 2 3
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 1 0
Mitochondria – 13 7 21 18 33 69 70 55 78 95 144 1 0 4 6 7 4 0 16 15 2 3 7
Nucleus – 2 0 0 2 0 0 1 0 4 1 0 0 1 1 3 3 2 3 3 0 1 0 0
Nuclear Periphery – 1 0 0 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus – 0 2 0 0 0 0 0 1 0 1 0 0 0 1 0 2 0 1 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole – 1 0 0 1 0 0 3 0 2 4 4 0 0 0 0 0 1 0 3 1 0 4 5
Vac/Vac Membrane – 18 1 0 9 0 0 2 0 0 1 4 7 0 1 8 11 8 5 15 10 10 5 6
Unique Cell Count 321 194 55 258 201 257 282 102 189 165 269 142 76 119 94 85 84 38 342 197 97 103 115
Labelled Cell Count 335 198 62 274 224 302 333 135 244 208 357 145 77 125 104 93 97 38 342 197 97 103 115


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 3.5 3.2 3.0 3.4 2.9 2.8 2.9 2.6 2.5 2.4 2.3 4.5 4.3 4.0 6.0 5.4 6.5 4.2 4.5 5.1
Std Deviation (1e-4) – 0.5 0.7 0.8 1.2 1.2 1.0 1.3 1.5 1.3 0.8 0.7 1.1 1.0 1.3 1.9 1.1 2.7 1.3 1.0 1.9
Intensity Change (Log2) – – – -0.1 0.08 -0.14 -0.2 -0.14 -0.29 -0.36 -0.4 -0.49 0.48 0.43 0.3 0.9 0.76 1.02 0.38 0.47 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.669 0.2455 0.3383 0.0808 -0.0659 0.2654 -0.7957 -1.1237 -1.0104 -1.0875 0.2285 -0.8263 3.7988 2.9578 3.1274 2.5851 2.1798 3.1065
Actin 0.0915 0.0004 0.0082 0.0085 0.0315 0.0022 0.0273 0.0012 0.0105 0.0024 0.0114 0.0132 0.0691 0.0002 0.0034 0.0302 0.0003 0.0067
Bud 0.0004 0.0007 0.0003 0.0003 0.0016 0.0006 0.0054 0.0229 0.0236 0.0061 0.0338 0.0095 0.0018 0.0002 0.001 0.0002 0 0.0005
Bud Neck 0.0022 0.0005 0.0006 0.0007 0.0021 0.0077 0.0069 0.0012 0.0015 0.0007 0.004 0.0096 0.006 0.0001 0.0003 0.0003 0.0001 0.0022
Bud Periphery 0.0007 0.0002 0.0005 0.0004 0.0098 0.0028 0.0033 0.0052 0.0094 0.0065 0.0233 0.0081 0.0053 0.0003 0.0008 0.0002 0 0.0008
Bud Site 0.0014 0.005 0.0016 0.0006 0.0017 0.0004 0.0133 0.0454 0.018 0.0182 0.0091 0.0018 0.0074 0.0005 0.0011 0.0003 0.0001 0.0048
Cell Periphery 0.0003 0.0002 0.0002 0.0001 0.0031 0.0001 0.0005 0.0008 0.0006 0.0002 0.0015 0.0004 0.0004 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.2076 0.4102 0.3035 0.4178 0.2871 0.4328 0.2219 0.2708 0.325 0.2857 0.2523 0.3201 0.2098 0.3321 0.2763 0.1283 0.2763 0.3114
Cytoplasmic Foci 0.0186 0.0096 0.0164 0.0157 0.0157 0.0156 0.0418 0.0284 0.0165 0.0242 0.0297 0.0505 0.0243 0.0062 0.0101 0.0093 0.01 0.0152
Eisosomes 0.0012 0.0001 0.0002 0.0001 0.0005 0.0001 0.0004 0.0002 0.0002 0.0001 0.0007 0.0001 0.0007 0.0001 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0048 0.0049 0.0066 0.0029 0.0443 0.0036 0.0066 0.0033 0.0065 0.0023 0.0061 0.0046 0.0094 0.0036 0.003 0.0015 0.003 0.0036
Endosome 0.025 0.0131 0.0137 0.0089 0.0193 0.007 0.0273 0.0046 0.0067 0.0047 0.0198 0.0086 0.0171 0.0034 0.0023 0.0013 0.0044 0.0043
Golgi 0.0092 0.0002 0.0044 0.0016 0.0084 0.0019 0.0073 0.0003 0.001 0.0003 0.0037 0.0017 0.0062 0.0002 0.0002 0.0005 0.0002 0.0006
Lipid Particles 0.0129 0.001 0.0176 0.0026 0.0151 0.0061 0.0124 0.0009 0.0017 0.0009 0.0241 0.011 0.023 0.0003 0.0002 0.0007 0.0005 0.0006
Mitochondria 0.0062 0.0005 0.0062 0.0013 0.0049 0.0027 0.0037 0.0016 0.0023 0.0038 0.0374 0.0032 0.0029 0.0003 0.0006 0.001 0.0005 0.0005
None 0.4823 0.4789 0.5717 0.4828 0.5081 0.4062 0.4805 0.4761 0.4501 0.5715 0.3765 0.259 0.5113 0.6332 0.6548 0.6652 0.6934 0.5864
Nuclear Periphery 0.0296 0.005 0.0068 0.0064 0.0129 0.0074 0.0197 0.0067 0.0134 0.0052 0.0398 0.0146 0.0188 0.0017 0.0034 0.0029 0.0019 0.0032
Nucleolus 0.0017 0.0009 0.001 0.0006 0.0003 0.0024 0.0041 0.0062 0.0037 0.0025 0.0179 0.0056 0.0024 0.0002 0.0003 0.0012 0.0002 0.0006
Nucleus 0.0659 0.0551 0.0214 0.0279 0.0055 0.0734 0.0577 0.0748 0.062 0.0402 0.0484 0.1774 0.0555 0.0133 0.0342 0.0898 0.0062 0.0406
Peroxisomes 0.0077 0.0002 0.0063 0.0026 0.0116 0.0013 0.0025 0.0005 0.0013 0.0009 0.0039 0.0087 0.0119 0.0001 0.0009 0.0553 0.0002 0.0007
Punctate Nuclear 0.0256 0.0054 0.0088 0.0167 0.0072 0.024 0.0515 0.0458 0.0419 0.0212 0.0413 0.0867 0.0125 0.0021 0.0059 0.0111 0.0016 0.0161
Vacuole 0.0033 0.0066 0.0028 0.001 0.0079 0.0014 0.004 0.0027 0.0034 0.002 0.0096 0.0043 0.0028 0.0016 0.0009 0.0005 0.0009 0.0009
Vacuole Periphery 0.0018 0.0013 0.001 0.0004 0.0013 0.0004 0.0018 0.0004 0.0008 0.0005 0.0055 0.0013 0.0012 0.0001 0.0002 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9553 16.4543 12.8639 22.0587 17.6856 13.4687 19.3107 19.5501 19.1017 22.6821
Translational Efficiency 0.2303 0.2156 0.3274 0.2143 0.2133 0.3127 0.1941 0.2043 0.2282 0.2043

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
477 1468 544 1458 1172 1757 287 157 1649 3225 831 1615

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 615.36 717.64 965.29 795.80 589.60 690.90 892.52 855.91 597.05 703.07 940.16 801.64
Standard Deviation 59.18 96.09 101.76 100.82 89.04 78.29 98.58 87.74 82.36 87.86 106.45 101.20
Intensity Change Log 2 — 0.221830 0.649532 0.370975 — 0.228740 0.598148 0.537723 — 0.225215 0.624618 0.454975

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000041 0.000210 0.000717 0.000753 0.000064 0.000447 0.000469 0.000309 0.000057 0.000339 0.000631 0.000710
Bud Neck 0.007902 0.025009 0.004843 0.008498 0.006565 0.025330 0.006141 0.023621 0.006952 0.025184 0.005291 0.009968
Bud Site 0.001553 0.007092 0.010340 0.034852 0.001548 0.009732 0.014584 0.008923 0.001549 0.008531 0.011806 0.032331
Cell Periphery 0.000114 0.000166 0.000151 0.000185 0.000123 0.000145 0.000138 0.000201 0.000120 0.000155 0.000147 0.000186
Cytoplasm 0.444763* 0.399122* 0.122377* 0.342613* 0.422624* 0.410955* 0.108562* 0.256217* 0.429028* 0.405569* 0.117606* 0.334214*
Cytoplasmic Foci 0.183992* 0.181690* 0.002551 0.008191 0.129806 0.167371 0.003507 0.021814 0.145480 0.173889 0.002881 0.009515
Eisosomes 0.000118 0.000103 0.000045 0.000038 0.000083 0.000099 0.000057 0.000082 0.000093 0.000101 0.000049 0.000042
Endoplasmic Reticulum 0.000350 0.001000 0.008854 0.003174 0.000625 0.000679 0.007697 0.006071 0.000545 0.000825 0.008455 0.003456
Endosome 0.006366 0.012033 0.001818 0.002789 0.004796 0.009992 0.001580 0.006816 0.005250 0.010921 0.001736 0.003181
Golgi 0.000864 0.002247 0.000082 0.000260 0.000716 0.003102 0.000065 0.000086 0.000759 0.002713 0.000076 0.000243
Lipid Particles 0.009727 0.006646 0.001373 0.001096 0.009978 0.007973 0.001739 0.002310 0.009906 0.007369 0.001499 0.001214
Mitochondria 0.004534 0.010045 0.000777 0.005862 0.003700 0.009949 0.000734 0.001896 0.003941 0.009993 0.000762 0.005476
Mitotic Spindle 0.001013 0.003333 0.002314 0.033912 0.000767 0.009247 0.000449 0.003710 0.000838 0.006555 0.001670 0.030976
None 0.005178 0.005023 0.004406 0.008948 0.013789 0.004711 0.003175 0.005710 0.011298 0.004853 0.003981 0.008634
Nuclear Periphery 0.000656 0.001094 0.002066 0.001999 0.000889 0.000714 0.001828 0.002657 0.000821 0.000887 0.001984 0.002063
Nuclear Periphery Foci 0.000266 0.000844 0.001565 0.000837 0.000526 0.000581 0.001081 0.001931 0.000451 0.000701 0.001398 0.000943
Nucleolus 0.001899 0.003131 0.000741 0.000499 0.003245 0.002947 0.000637 0.000527 0.002856 0.003031 0.000705 0.000502
Nucleus 0.298415* 0.280328* 0.796721* 0.474693* 0.375048* 0.278994* 0.807186* 0.425907* 0.352880* 0.279601* 0.800336* 0.469951*
Peroxisomes 0.003993 0.011717 0.000236 0.000361 0.003377 0.014603 0.000266 0.000901 0.003555 0.013289 0.000246 0.000413
Vacuole 0.027725 0.047775 0.037855 0.067706 0.021257 0.041283 0.039957 0.228145* 0.023128 0.044238 0.038581 0.083303
Vacuole Periphery 0.000533 0.001391 0.000167 0.002734 0.000476 0.001145 0.000147 0.002165 0.000492 0.001257 0.000160 0.002679

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.01 -5.49 -3.80 -2.72 1.78 -2.24 -8.67 -6.74 -0.94 -0.30 -3.02 -6.71 -4.05 -1.86 1.39
Bud Neck -7.82 1.32 -0.85 7.81 -2.81 -9.81 0.57 -1.24 3.83 -1.46 -12.49 0.99 -2.03 10.85 -3.17
Bud Site -5.00 -2.89 -6.87 -4.56 -2.60 -7.03 -1.96 -3.31 1.40 0.56 -8.85 -3.53 -7.32 -3.96 -2.63
Cell Periphery -1.87 -4.77 -3.16 0.59 2.07 -0.89 -2.22 -2.04 -0.57 -0.10 -1.86 -4.83 -2.86 0.22 1.81
Cytoplasm 3.45 26.22 14.63 15.01 -16.25 1.03 26.31 13.51 13.39 -3.98 2.86 38.70 20.34 20.51 -17.53
Cytoplasmic Foci -0.03 22.21 21.70 32.44 -5.76 -5.72 26.23 22.81 30.77 -3.25 -5.39 34.26 33.21 47.21 -5.72
Eisosomes 1.53 5.90 7.98 8.94 1.92 -2.46 0.43 -3.03 -1.64 -2.83 -1.45 4.35 7.94 10.02 2.01
Endoplasmic Reticulum -5.83 -14.90 -23.58 -19.18 8.62 -0.64 -11.02 -8.99 -8.94 0.13 -4.17 -18.10 -24.32 -22.65 9.07
Endosome -3.52 3.00 3.57 10.30 2.07 -5.34 4.11 0.36 4.44 -2.23 -6.82 4.83 5.23 13.82 0.99
Golgi -2.56 2.22 2.14 5.11 -0.64 -3.46 3.66 3.37 4.50 -1.58 -4.44 4.18 3.88 6.33 -1.01
Lipid Particles 2.20 5.76 6.15 13.94 2.19 1.82 8.24 7.75 6.54 -1.21 2.98 10.25 10.96 12.72 2.35
Mitochondria -3.30 3.11 1.21 4.96 -2.30 -4.83 4.71 4.18 7.92 -0.67 -6.06 5.53 1.45 6.68 -2.40
Mitotic Spindle -0.28 -0.34 -5.85 -6.73 -6.27 -5.04 0.27 -2.44 -0.09 -2.48 -4.46 -1.18 -7.76 -5.67 -7.09
None 0.05 1.75 -1.16 -1.31 -3.91 5.74 7.36 2.72 -1.33 -2.34 5.51 7.23 4.09 -2.13 -5.01
Nuclear Periphery -1.24 -14.21 -15.77 -7.96 -4.60 1.65 -8.98 -5.79 -6.12 -3.28 -0.27 -15.54 -16.33 -13.70 -6.08
Nuclear Periphery Foci -3.31 -6.66 -5.31 -2.69 1.90 -0.12 -4.23 -3.36 -3.31 -2.16 -1.88 -7.31 -5.53 -4.32 0.87
Nucleolus -2.88 3.52 4.25 6.80 2.07 0.74 6.05 3.31 2.77 -0.97 -0.43 6.91 7.32 8.78 1.32
Nucleus 1.51 -32.09 -17.14 -23.58 18.73 9.80 -24.87 -3.12 -7.19 11.76 9.48 -39.75 -16.43 -27.11 22.49
Peroxisomes -5.80 4.72 4.84 11.36 1.44 -7.96 7.29 6.58 10.55 -0.72 -10.21 8.72 8.94 15.20 1.20
Vacuole -5.11 -6.20 -17.69 -14.96 -8.52 -9.35 -6.73 -11.65 -10.76 -9.04 -11.01 -9.85 -22.94 -18.98 -11.47
Vacuole Periphery -3.20 4.37 -0.87 0.82 -1.58 -4.00 5.29 -0.09 1.91 -1.20 -5.00 6.35 -0.96 0.77 -1.69
Standard name
Human Ortholog
Description hEST1A/B (SMG5/6)-like protein; contributes to environment-sensing adaptive gene expression responses; Esl1p and Esl2p contain a 14-3-3-like domain and a putative PilT N-terminus ribonuclease domain; ESL1 has a paralog, ESL2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (14%), nucleus (5%), mixed (44%)
Cell Cycle Regulation Yes
cytoplasm - MA (6.5e-06)
nucleus - T/G1 (0.00054)
Subcompartmental Group N/A

Esl1

Esl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Esl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available