Standard name | |
---|---|
Human Ortholog | |
Description | Integral plasma membrane protein; required for axial budding in haploid cells; localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 44 | 70 | 36 | 21 | 6 | 16 | – | 10 | 23 | 15 | 10 | 11 | 59 | 41 | 52 | 47 | 13 | 20 | 48 | 37 | 39 | 15 | 9 | 8 |
Bud | 14 | 14 | 20 | 13 | 7 | 14 | – | 59 | 45 | 53 | 39 | 48 | 18 | 11 | 13 | 9 | 3 | 2 | 35 | 33 | 23 | 9 | 11 | 10 |
Bud Neck | 0 | 0 | 1 | 3 | 0 | 0 | – | 1 | 1 | 0 | 1 | 1 | 23 | 16 | 42 | 1 | 1 | 0 | 113 | 72 | 106 | 45 | 26 | 10 |
Bud Site | 49 | 44 | 36 | 29 | 15 | 55 | – | 51 | 56 | 45 | 47 | 46 | 54 | 47 | 50 | 29 | 7 | 4 | – | – | – | – | – | – |
Cell Periphery | 4 | 0 | 1 | 0 | 1 | 2 | – | 3 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 30 | 28 | 35 | 38 | 65 | 118 | – | 191 | 175 | 175 | 204 | 228 | 36 | 15 | 38 | 71 | 63 | 42 | 0 | 3 | 3 | 2 | 7 | 18 |
Endoplasmic Reticulum | 5 | 5 | 1 | 2 | 5 | 9 | – | 6 | 10 | 6 | 16 | 4 | 3 | 1 | 4 | 19 | 12 | 6 | 4 | 4 | 4 | 2 | 11 | 13 |
Endosome | 49 | 7 | 36 | 18 | 56 | 54 | – | 75 | 9 | 21 | 10 | 19 | 2 | 2 | 6 | 19 | 15 | 7 | 45 | 25 | 32 | 9 | 17 | 16 |
Golgi | 5 | 2 | 2 | 2 | 1 | 0 | – | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 7 | 5 | 9 | 5 | 2 | 1 | 2 | 4 |
Mitochondria | 57 | 54 | 39 | 33 | 35 | 83 | – | 127 | 179 | 208 | 154 | 203 | 1 | 1 | 0 | 52 | 26 | 13 | 27 | 10 | 14 | 10 | 23 | 26 |
Nucleus | 12 | 2 | 1 | 2 | 5 | 13 | – | 26 | 10 | 11 | 10 | 14 | 4 | 11 | 20 | 13 | 13 | 6 | 0 | 2 | 2 | 0 | 1 | 0 |
Nuclear Periphery | 1 | 3 | 2 | 1 | 2 | 2 | – | 8 | 2 | 10 | 5 | 9 | 0 | 1 | 2 | 0 | 0 | 0 | 4 | 2 | 9 | 2 | 2 | 4 |
Nucleolus | 17 | 7 | 1 | 7 | 20 | 36 | – | 77 | 26 | 42 | 17 | 58 | 3 | 4 | 3 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 94 | 45 | 120 | 64 | 66 | 86 | – | 42 | 54 | 54 | 28 | 41 | 26 | 20 | 38 | 76 | 20 | 22 | 39 | 20 | 28 | 1 | 2 | 0 |
SpindlePole | 39 | 5 | 42 | 22 | 16 | 32 | – | 63 | 15 | 30 | 21 | 41 | 68 | 91 | 106 | 62 | 34 | 22 | 2 | 4 | 4 | 0 | 0 | 1 |
Vac/Vac Membrane | 153 | 92 | 99 | 54 | 157 | 144 | – | 209 | 132 | 156 | 78 | 104 | 8 | 2 | 9 | 138 | 98 | 48 | 303 | 195 | 203 | 100 | 258 | 262 |
Unique Cell Count | 431 | 274 | 342 | 222 | 365 | 473 | 707 | 565 | 619 | 495 | 633 | 203 | 172 | 274 | 404 | 249 | 156 | 638 | 420 | 479 | 203 | 377 | 379 | |
Labelled Cell Count | 573 | 378 | 472 | 309 | 457 | 664 | 949 | 741 | 827 | 640 | 829 | 306 | 263 | 383 | 545 | 314 | 199 | 638 | 420 | 479 | 203 | 377 | 379 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 6.9 | 5.7 | 5.6 | 6.0 | 5.2 | – | 5.2 | 4.8 | 4.6 | 4.5 | 4.5 | 6.5 | 6.3 | 6.3 | 6.0 | 6.7 | 7.6 | 6.9 | 6.7 | 6.7 |
Std Deviation (1e-4) | 1.5 | 1.3 | 1.4 | 1.3 | 1.7 | 1.5 | – | 1.4 | 1.3 | 1.3 | 1.2 | 1.2 | 1.3 | 1.7 | 1.2 | 1.3 | 1.8 | 2.0 | 1.9 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.06 | -0.16 | – | -0.14 | -0.27 | -0.33 | -0.36 | -0.36 | 0.19 | 0.12 | 0.14 | 0.07 | 0.23 | 0.41 | 0.26 | 0.23 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.4 | -5.0 | -4.1 | – | -6.8 | 0 | 0 | 0 | 0 | 5.5 | 4.0 | 3.0 | 0.5 | -2.3 | 0.8 |
Bud | 0 | -2.7 | -2.0 | – | 1.4 | 0 | 0 | 0 | 0 | 1.3 | 0.2 | -0.6 | -2.5 | -2.9 | -2.3 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 6.1 | 5.4 | 7.3 | 0 | 0 | 0 |
Bud Site | 0.9 | -3.3 | 0.5 | – | -1.8 | 0 | 0 | 0 | 0 | 4.9 | 4.9 | 2.7 | -1.6 | -3.6 | -3.0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.4 | 2.9 | 5.3 | – | 6.2 | 7.2 | 6.5 | 9.8 | 8.7 | 2.5 | -0.5 | 1.4 | 2.9 | 4.9 | 4.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.7 | 0 |
Endosome | -1.0 | 1.9 | 0.4 | – | 0 | 0 | 0 | 0 | 0 | -4.2 | -3.8 | -4.1 | -3.0 | -1.9 | -2.2 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.2 | -0.8 | 2.4 | – | 2.7 | 0 | 0 | 0 | 0 | -4.7 | -4.3 | -5.8 | 0.6 | -0.4 | -1.0 |
Nucleus | 0 | 0 | 2.7 | – | 3.2 | 0 | 0 | 0 | 2.3 | 0 | 0 | 4.8 | 2.9 | 3.9 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 4.1 | 5.0 | – | 6.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -1.5 | -5.1 | -5.5 | – | -12.2 | 0 | 0 | 0 | 0 | -5.7 | -5.6 | -6.0 | -5.0 | -7.6 | -4.8 |
SpindlePole | -0.9 | -3.8 | -2.7 | – | -1.7 | 0 | 0 | 0 | 0 | 6.0 | 9.9 | 7.6 | 1.2 | 0.5 | 0.6 |
Vacuole | -1.2 | 3.9 | 0.5 | – | 0.2 | -1.9 | -1.3 | -4.6 | -4.6 | -7.1 | -7.5 | -8.3 | 1.5 | 2.7 | 0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1895 | 3.8635 | 3.9822 | 3.2343 | 2.7589 | 3.4781 | 3.4871 | 4.7937 | 5.5495 | 4.8517 | 4.4772 | 4.765 | 3.4544 | 5.9831 | 6.1805 | 5.5991 | 5.5361 | 5.5214 |
Actin | 0.0924 | 0.0142 | 0.0596 | 0.0019 | 0.0274 | 0.0251 | 0.0444 | 0.0044 | 0.0212 | 0.0004 | 0.0015 | 0.0317 | 0.04 | 0.0253 | 0.0223 | 0.0036 | 0.0076 | 0.0108 |
Bud | 0.0086 | 0.009 | 0.0051 | 0.0018 | 0.0024 | 0.0023 | 0.0091 | 0.0068 | 0.004 | 0.0005 | 0.0008 | 0.0021 | 0.0111 | 0.0049 | 0.0029 | 0.0008 | 0.0011 | 0.0018 |
Bud Neck | 0.1997 | 0.116 | 0.5734 | 0.8547 | 0.6906 | 0.7354 | 0.3477 | 0.1143 | 0.5913 | 0.9071 | 0.9592 | 0.8087 | 0.3059 | 0.1151 | 0.6253 | 0.9634 | 0.8448 | 0.7798 |
Bud Periphery | 0.0167 | 0.0173 | 0.0087 | 0.0041 | 0.0068 | 0.0061 | 0.0227 | 0.0088 | 0.0083 | 0.0012 | 0.0022 | 0.0067 | 0.0203 | 0.0068 | 0.0058 | 0.002 | 0.0033 | 0.0048 |
Bud Site | 0.1541 | 0.5194 | 0.1712 | 0.0107 | 0.0219 | 0.0064 | 0.1822 | 0.6638 | 0.1969 | 0.0039 | 0.0113 | 0.0055 | 0.3081 | 0.5247 | 0.1554 | 0.0063 | 0.0044 | 0.0117 |
Cell Periphery | 0.004 | 0.0081 | 0.0067 | 0.0032 | 0.0061 | 0.0034 | 0.0067 | 0.007 | 0.0048 | 0.0017 | 0.0026 | 0.0028 | 0.0063 | 0.0073 | 0.0069 | 0.0016 | 0.005 | 0.0048 |
Cytoplasm | 0.0195 | 0.0348 | 0.0029 | 0.0013 | 0.0022 | 0.0049 | 0.0199 | 0.0264 | 0.006 | 0.0011 | 0.0009 | 0.0058 | 0.0359 | 0.0386 | 0.0053 | 0.0007 | 0.0018 | 0.0178 |
Cytoplasmic Foci | 0.1053 | 0.0375 | 0.0254 | 0.011 | 0.0132 | 0.0228 | 0.0815 | 0.0159 | 0.0249 | 0.0043 | 0.0027 | 0.0136 | 0.0358 | 0.0126 | 0.0144 | 0.0041 | 0.026 | 0.0093 |
Eisosomes | 0.0007 | 0.0002 | 0.0006 | 0.0001 | 0.0013 | 0.0001 | 0.0013 | 0.0002 | 0.0003 | 0 | 0 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0129 | 0.0019 | 0.0011 | 0.0003 | 0.002 | 0.0013 | 0.0198 | 0.0021 | 0.001 | 0.0002 | 0.0001 | 0.001 | 0.0027 | 0.0055 | 0.0011 | 0.0001 | 0.0003 | 0.0018 |
Endosome | 0.11 | 0.0442 | 0.0251 | 0.0267 | 0.0548 | 0.0419 | 0.0609 | 0.0232 | 0.024 | 0.0154 | 0.0033 | 0.0194 | 0.0409 | 0.0329 | 0.0343 | 0.0039 | 0.0276 | 0.0249 |
Golgi | 0.0411 | 0.0131 | 0.0175 | 0.0137 | 0.0275 | 0.0255 | 0.016 | 0.0039 | 0.0122 | 0.0031 | 0.0023 | 0.0128 | 0.0073 | 0.0045 | 0.0129 | 0.0041 | 0.0131 | 0.0067 |
Lipid Particles | 0.0482 | 0.0081 | 0.0173 | 0.0109 | 0.0107 | 0.0105 | 0.0204 | 0.0024 | 0.0085 | 0.0016 | 0.0005 | 0.0015 | 0.005 | 0.0039 | 0.005 | 0.0009 | 0.0078 | 0.0018 |
Mitochondria | 0.0135 | 0.0106 | 0.0094 | 0.0125 | 0.0492 | 0.0057 | 0.0117 | 0.0045 | 0.0038 | 0.0012 | 0.0005 | 0.0056 | 0.0041 | 0.0026 | 0.0043 | 0.0003 | 0.0044 | 0.0027 |
None | 0.0065 | 0.0033 | 0.0007 | 0.0002 | 0.0009 | 0.0004 | 0.0441 | 0.0389 | 0.017 | 0 | 0.0001 | 0.024 | 0.0426 | 0.0228 | 0.002 | 0 | 0.0005 | 0.0031 |
Nuclear Periphery | 0.0527 | 0.0014 | 0.0007 | 0.0002 | 0.0026 | 0.0012 | 0.0277 | 0.0014 | 0.0018 | 0.0001 | 0 | 0.0004 | 0.0013 | 0.0048 | 0.0005 | 0 | 0.0001 | 0.0006 |
Nucleolus | 0.0063 | 0.0074 | 0.0055 | 0.0089 | 0.0038 | 0.0018 | 0.0053 | 0.0032 | 0.0039 | 0.003 | 0.0036 | 0.0016 | 0.0035 | 0.0054 | 0.0058 | 0.0017 | 0.0036 | 0.0026 |
Nucleus | 0.0116 | 0.0047 | 0.0023 | 0.0021 | 0.0027 | 0.0016 | 0.0078 | 0.0034 | 0.0053 | 0.0013 | 0.0009 | 0.0012 | 0.0035 | 0.0114 | 0.0024 | 0.0006 | 0.0016 | 0.0018 |
Peroxisomes | 0.0348 | 0.0071 | 0.0262 | 0.0052 | 0.0036 | 0.0071 | 0.0172 | 0.0031 | 0.0079 | 0.0007 | 0.0007 | 0.0031 | 0.0078 | 0.0023 | 0.0031 | 0.0012 | 0.0099 | 0.0021 |
Punctate Nuclear | 0.0094 | 0.0012 | 0.0013 | 0.0005 | 0.0011 | 0.001 | 0.006 | 0.0012 | 0.0038 | 0.0002 | 0.0002 | 0.0004 | 0.0017 | 0.0035 | 0.0007 | 0.0003 | 0.0008 | 0.0003 |
Vacuole | 0.045 | 0.1342 | 0.0357 | 0.0263 | 0.0561 | 0.0872 | 0.0421 | 0.0615 | 0.049 | 0.0478 | 0.006 | 0.0463 | 0.1071 | 0.1605 | 0.0831 | 0.0038 | 0.0338 | 0.1019 |
Vacuole Periphery | 0.007 | 0.0063 | 0.0036 | 0.0036 | 0.0131 | 0.0083 | 0.0053 | 0.0033 | 0.0041 | 0.005 | 0.0004 | 0.0056 | 0.0086 | 0.0044 | 0.0061 | 0.0003 | 0.0023 | 0.0087 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 39.9493 | 8.7784 | 2.9609 | 9.2635 | 37.3279 | 32.1202 | 12.9103 | 8.0329 | 7.2491 | 41.9323 |
Translational Efficiency | 1.6513 | 1.3903 | 1.1089 | 0.789 | 1.3831 | 2.3131 | 1.1154 | 1.0435 | 1.051 | 1.3674 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
182 | 1056 | 1837 | 468 | 2118 | 1553 | 2217 | 940 | 2300 | 2609 | 4054 | 1408 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 923.18 | 772.72 | 878.06 | 1024.04 | 777.20 | 802.39 | 835.04 | 998.39 | 788.75 | 790.38 | 854.53 | 1006.92 |
Standard Deviation | 136.95 | 116.24 | 113.05 | 171.54 | 99.59 | 107.05 | 130.88 | 169.82 | 110.32 | 111.81 | 124.97 | 170.82 |
Intensity Change Log 2 | — | -0.256666 | -0.072292 | 0.149588 | — | 0.046018 | 0.103559 | 0.361318 | — | -0.110405 | 0.010752 | 0.250233 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.007791 | 0.003883 | 0.005009 | 0.042985 | 0.002914 | 0.004824 | 0.003669 | 0.025297 | 0.003300 | 0.004443 | 0.004276 | 0.031176 |
Bud Neck | 0.121276 | 0.174873 | 0.280466* | 0.155930 | 0.330920* | 0.306144* | 0.207234* | 0.171878 | 0.314331* | 0.253012* | 0.240418* | 0.166577 |
Bud Site | 0.561021* | 0.452380* | 0.475144* | 0.681403* | 0.421693* | 0.400113* | 0.625374* | 0.694458* | 0.432718* | 0.421268* | 0.557300* | 0.690119* |
Cell Periphery | 0.001945 | 0.005936 | 0.003102 | 0.022087 | 0.005338 | 0.010438 | 0.002042 | 0.022345 | 0.005070 | 0.008616 | 0.002522 | 0.022259 |
Cytoplasm | 0.084421 | 0.069845 | 0.100765* | 0.034743 | 0.041895 | 0.039496 | 0.080757 | 0.038378 | 0.045260 | 0.051780 | 0.089824 | 0.037170 |
Cytoplasmic Foci | 0.020536 | 0.024283 | 0.013101 | 0.004230 | 0.010340 | 0.013414 | 0.006423 | 0.002827 | 0.011147 | 0.017813 | 0.009449 | 0.003294 |
Eisosomes | 0.000141 | 0.000127 | 0.000169 | 0.002029 | 0.000137 | 0.000213 | 0.000089 | 0.000712 | 0.000138 | 0.000178 | 0.000126 | 0.001150 |
Endoplasmic Reticulum | 0.001759 | 0.001990 | 0.000981 | 0.001246 | 0.001013 | 0.001579 | 0.000506 | 0.001892 | 0.001072 | 0.001745 | 0.000721 | 0.001677 |
Endosome | 0.022146 | 0.060041 | 0.009429 | 0.004012 | 0.039676 | 0.043635 | 0.009018 | 0.004227 | 0.038289 | 0.050275 | 0.009204 | 0.004155 |
Golgi | 0.014746 | 0.011251 | 0.001081 | 0.002454 | 0.003926 | 0.006836 | 0.001889 | 0.001347 | 0.004782 | 0.008623 | 0.001523 | 0.001715 |
Lipid Particles | 0.001631 | 0.001412 | 0.000995 | 0.000286 | 0.000412 | 0.001165 | 0.000240 | 0.000843 | 0.000508 | 0.001265 | 0.000582 | 0.000658 |
Mitochondria | 0.004274 | 0.019313 | 0.001377 | 0.003628 | 0.010436 | 0.018988 | 0.005019 | 0.004241 | 0.009948 | 0.019120 | 0.003369 | 0.004037 |
Mitotic Spindle | 0.006608 | 0.004664 | 0.003604 | 0.003704 | 0.001834 | 0.001455 | 0.012650 | 0.003806 | 0.002211 | 0.002754 | 0.008551 | 0.003772 |
None | 0.000097 | 0.000566 | 0.000498 | 0.000028 | 0.000253 | 0.000117 | 0.000871 | 0.000057 | 0.000241 | 0.000299 | 0.000702 | 0.000048 |
Nuclear Periphery | 0.000268 | 0.000066 | 0.000563 | 0.000062 | 0.000018 | 0.000034 | 0.000051 | 0.000283 | 0.000038 | 0.000047 | 0.000283 | 0.000210 |
Nuclear Periphery Foci | 0.000706 | 0.000326 | 0.000985 | 0.000270 | 0.000059 | 0.000092 | 0.000198 | 0.000299 | 0.000110 | 0.000187 | 0.000554 | 0.000289 |
Nucleolus | 0.000235 | 0.000328 | 0.000371 | 0.000018 | 0.000179 | 0.000349 | 0.000257 | 0.000019 | 0.000183 | 0.000341 | 0.000309 | 0.000019 |
Nucleus | 0.009208 | 0.002752 | 0.029983 | 0.002933 | 0.001985 | 0.001676 | 0.008810 | 0.001054 | 0.002556 | 0.002111 | 0.018404 | 0.001679 |
Peroxisomes | 0.008331 | 0.015425 | 0.012788 | 0.005285 | 0.006962 | 0.009248 | 0.006062 | 0.004608 | 0.007070 | 0.011748 | 0.009110 | 0.004833 |
Vacuole | 0.127482 | 0.140047 | 0.058916 | 0.031831 | 0.113207 | 0.133515 | 0.027901 | 0.019756 | 0.114337 | 0.136159 | 0.041955 | 0.023769 |
Vacuole Periphery | 0.005377 | 0.010492 | 0.000671 | 0.000836 | 0.006804 | 0.006669 | 0.000939 | 0.001671 | 0.006691 | 0.008216 | 0.000817 | 0.001394 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.47 | 0.88 | -6.42 | -7.51 | -7.26 | -1.96 | -1.27 | -8.81 | -7.78 | -8.49 | -1.69 | -1.97 | -11.41 | -10.85 | -11.03 |
Bud Neck | -4.40 | -10.24 | -2.57 | 2.03 | 9.60 | 2.43 | 15.55 | 15.72 | 12.50 | 3.74 | 7.07 | 10.50 | 16.82 | 10.84 | 9.41 |
Bud Site | 1.22 | 0.95 | -5.39 | -11.86 | -12.21 | 2.12 | -21.08 | -22.37 | -22.71 | -6.33 | 0.67 | -14.27 | -24.11 | -25.05 | -14.04 |
Cell Periphery | -3.70 | -1.40 | -4.73 | -3.69 | -4.46 | -5.73 | 7.46 | -5.49 | -3.68 | -6.65 | -5.20 | 5.87 | -6.82 | -5.25 | -7.95 |
Cytoplasm | 1.72 | -1.64 | 4.58 | 5.38 | 11.18 | 0.69 | -10.61 | 0.21 | -0.36 | 9.29 | -1.94 | -14.04 | 1.74 | 3.43 | 14.45 |
Cytoplasmic Foci | -0.82 | 1.92 | 4.03 | 7.89 | 4.51 | -1.88 | 2.69 | 6.59 | 7.24 | 4.31 | -4.66 | 0.87 | 6.58 | 11.10 | 6.99 |
Eisosomes | 0.49 | -1.13 | -3.17 | -3.20 | -3.12 | -3.21 | 3.52 | -5.93 | -5.05 | -6.42 | -2.48 | 0.91 | -4.91 | -4.70 | -4.96 |
Endoplasmic Reticulum | -0.25 | 1.25 | 0.46 | 0.87 | -1.49 | -1.47 | 1.30 | -1.08 | -0.48 | -1.57 | -1.83 | 0.84 | -1.18 | -0.17 | -1.69 |
Endosome | -7.15 | 2.46 | 3.66 | 13.89 | 3.83 | -1.23 | 14.32 | 16.30 | 14.46 | 3.55 | -4.28 | 15.07 | 17.21 | 19.96 | 5.13 |
Golgi | 0.22 | 2.40 | 2.07 | 5.72 | -1.25 | -3.79 | 4.84 | 5.74 | 7.35 | 1.31 | -5.06 | 6.08 | 4.67 | 9.38 | -0.41 |
Lipid Particles | 0.32 | 0.85 | 1.74 | 2.36 | 2.81 | -1.85 | 1.03 | -0.80 | 0.38 | -0.99 | -2.55 | -1.12 | -0.62 | 1.03 | -0.26 |
Mitochondria | -5.49 | 1.17 | 0.07 | 5.95 | -1.28 | -3.90 | 4.98 | 4.31 | 6.44 | 0.62 | -5.44 | 7.15 | 4.75 | 8.74 | -0.57 |
Mitotic Spindle | 0.42 | 0.86 | 0.61 | 0.36 | -0.26 | 0.83 | -8.54 | -1.86 | -2.30 | 5.68 | -1.04 | -7.95 | -1.94 | -1.31 | 4.43 |
None | -2.45 | -5.46 | 1.55 | 2.87 | 7.36 | 1.06 | -2.15 | 1.53 | 3.02 | 3.08 | -0.39 | -2.56 | 1.61 | 3.28 | 4.47 |
Nuclear Periphery | 2.69 | -0.90 | 2.21 | -0.71 | 1.76 | -1.31 | -6.33 | -1.09 | -1.02 | -0.85 | -0.40 | -2.14 | -1.10 | -1.06 | 0.40 |
Nuclear Periphery Foci | 3.26 | -0.25 | 1.44 | -1.28 | 1.18 | -3.17 | -4.16 | -1.69 | -1.51 | -0.55 | -1.71 | -2.98 | -2.26 | -1.81 | 0.91 |
Nucleolus | -0.97 | -1.49 | 1.96 | 4.40 | 5.84 | -0.67 | -1.62 | 3.02 | 1.26 | 6.92 | -0.97 | -2.47 | 3.12 | 1.94 | 9.22 |
Nucleus | 3.30 | -5.16 | 2.02 | -1.44 | 8.96 | 0.64 | -7.70 | 0.72 | 0.06 | 8.10 | 1.54 | -13.95 | -0.17 | -1.22 | 12.86 |
Peroxisomes | -3.18 | -2.19 | 0.80 | 4.31 | 3.34 | -1.53 | 0.86 | 1.87 | 2.99 | 1.27 | -3.74 | -1.88 | 1.87 | 5.30 | 3.74 |
Vacuole | 3.10 | 6.18 | 6.54 | 7.72 | 1.77 | -2.62 | 15.29 | 13.59 | 14.20 | 0.68 | -2.19 | 13.72 | 13.67 | 16.10 | 2.75 |
Vacuole Periphery | -2.33 | 1.71 | 1.63 | 8.90 | -0.43 | 0.16 | 10.15 | 6.19 | 5.81 | -1.13 | -2.01 | 10.40 | 7.56 | 9.80 | -1.28 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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