Standard name
Human Ortholog
Description Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 – 0.05 – 0 0.07 0.11 0.1 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0 0.06 – 0 – 0 0.06 0.1 0.13 0.17 0.1 0.08 0.1 0.13 0.08 0.11 0 0 0 0 0 0
Cytoplasm 0.94 0.94 0.9 0.88 – 0.81 – 0.74 0.75 0.64 0.55 0.53 0.92 0.95 0.96 0.94 1.0 0.98 0.92 0.94 0.91 0.81 0.83 0.75
Endoplasmic Reticulum 0 0.07 0 0.06 – 0.06 – 0.08 0.07 0.14 0.1 0.16 0.06 0 0 0 0 0.05 0 0 0 0.07 0.07 0.11
Endosome 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0.14 – 0.18 0.29 0.26 0.43 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 – 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 4 2 0 – 3 – 3 6 9 10 18 2 2 0 0 0 1 5 1 1 3 2 5
Bud 1 1 1 0 – 0 – 1 1 0 0 2 2 1 1 0 0 0 6 1 0 1 3 6
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 4 0 0 1 0 2
Bud Site 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 11 13 1 1 – 1 – 0 5 8 13 20 21 12 22 19 7 12 0 1 0 0 0 1
Cytoplasm 521 216 35 15 – 52 – 54 63 51 54 62 196 146 215 139 89 103 537 218 65 103 165 168
Endoplasmic Reticulum 25 15 0 1 – 4 – 6 6 11 10 19 12 5 8 5 2 5 12 4 0 9 13 23
Endosome 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 2
Golgi 6 0 0 0 – 1 – 0 0 1 4 3 3 1 2 0 0 0 0 0 0 1 1 2
Mitochondria 3 0 0 0 – 9 – 13 24 21 42 36 1 0 0 1 2 1 0 0 0 0 2 1
Nucleus 0 0 0 0 – 0 – 1 1 1 0 0 0 0 0 0 1 0 0 0 0 1 1 0
Nuclear Periphery 0 0 0 0 – 0 – 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 1 1
Nucleolus 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 9 1 0 0 – 0 – 1 0 2 1 6 0 1 0 3 0 0 3 1 1 1 2 2
Unique Cell Count 556 230 39 17 64 73 84 80 98 117 214 153 224 148 89 105 581 232 72 128 200 223
Labelled Cell Count 581 250 39 17 70 79 106 104 134 167 237 168 248 167 101 122 581 232 72 128 200 223


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.8 22.1 11.7 14.3 – 13.5 – 12.9 11.8 12.1 11.9 12.5 18.3 18.7 18.8 23.5 21.9 22.0 18.5 19.7 19.3
Std Deviation (1e-4) 3.3 4.1 2.1 2.3 – 2.6 – 2.4 2.9 2.4 2.4 2.3 3.1 3.3 3.8 5.9 5.1 5.0 4.4 4.0 3.7
Intensity Change (Log2) – – – 0.28 – 0.2 – 0.13 0.01 0.04 0.02 0.09 0.64 0.67 0.68 1.0 0.9 0.91 0.65 0.75 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 – 0 0 0 0 1.7 0 0 0 0 0 0
Bud 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 – 0 – 0 0 0 0 2.3 0 0 0 0 0 0
Cytoplasm -0.2 – -1.2 – -2.0 -1.9 -3.0 -3.8 -4.1 0.4 1.4 1.7 0.9 3.1 2.2
Endoplasmic Reticulum 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 – 0 3.7 3.5 4.9 3.9 0 0 0 0 0 0
Nucleus 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.153 16.7509 15.0495 14.9417 16.0844 16.5229 11.6762 15.5947 14.4036 15.261 13.4358 14.6869 15.7996 17.9503 16.9363 16.4887 16.2982 17.7254
Actin 0.0346 0.0125 0.0352 0.05 0.0017 0.0065 0.0387 0.0152 0.0363 0.0257 0.0029 0.011 0.0376 0.0056 0.0212 0.0044 0.0133 0.0106
Bud 0.0073 0.0089 0.0288 0.0064 0.0009 0.0016 0.0115 0.0064 0.0234 0.0029 0.0008 0.0019 0.0085 0.0035 0.0208 0.0069 0.0042 0.0021
Bud Neck 0.0026 0.0018 0.0082 0.0052 0.0021 0.0443 0.005 0.0052 0.0059 0.0031 0.0009 0.0439 0.0038 0.0019 0.006 0.0047 0.0029 0.039
Bud Periphery 0.0051 0.0025 0.0146 0.0054 0.0002 0.0017 0.0079 0.0025 0.0243 0.0042 0.0004 0.0022 0.012 0.0013 0.0219 0.01 0.0025 0.0027
Bud Site 0.0174 0.0487 0.0157 0.0008 0.0004 0.0005 0.0213 0.0813 0.0335 0.0003 0.0012 0.0008 0.0306 0.0447 0.0198 0.0008 0.0007 0.0005
Cell Periphery 0.004 0.002 0.0011 0.0025 0.0002 0.0011 0.0026 0.0029 0.0026 0.0005 0.0005 0.0012 0.003 0.0016 0.0024 0.0014 0.0004 0.0011
Cytoplasm 0.7036 0.8511 0.7672 0.7487 0.8832 0.7671 0.638 0.8085 0.7737 0.8902 0.8326 0.7659 0.7165 0.871 0.802 0.8081 0.8401 0.7633
Cytoplasmic Foci 0.0212 0.0324 0.0157 0.0087 0.0317 0.0211 0.0454 0.0171 0.0126 0.0091 0.0273 0.0253 0.0211 0.0166 0.0119 0.0108 0.0129 0.0295
Eisosomes 0.0003 0 0.0002 0.0003 0 0 0.0003 0.0001 0.0001 0 0 0 0.0003 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0702 0.0069 0.017 0.0418 0.0074 0.0568 0.0519 0.0136 0.0107 0.0101 0.0188 0.0386 0.0683 0.0178 0.0289 0.0548 0.028 0.0396
Endosome 0.0492 0.014 0.0338 0.0508 0.0494 0.0518 0.0726 0.0177 0.0288 0.0277 0.0717 0.0509 0.0391 0.0158 0.0275 0.0529 0.0507 0.0574
Golgi 0.0225 0.0062 0.0174 0.0053 0.0056 0.0213 0.0278 0.009 0.011 0.0075 0.0157 0.0308 0.0227 0.0049 0.0129 0.0095 0.0205 0.0294
Lipid Particles 0.0036 0.0005 0.0046 0.0004 0.0002 0.0001 0.0091 0.0009 0.0044 0.0001 0.0015 0.0006 0.0038 0.0007 0.0004 0.0003 0.0001 0.0004
Mitochondria 0.0135 0.0009 0.0114 0.0078 0.0017 0.015 0.01 0.0015 0.0063 0.0021 0.0065 0.0113 0.0067 0.0012 0.0058 0.0041 0.0083 0.0133
None 0.0076 0.0005 0.0039 0.0005 0.0003 0.0005 0.0077 0.0006 0.0008 0.0003 0.0074 0.0009 0.0032 0.0005 0.0005 0.0006 0.0005 0.0009
Nuclear Periphery 0.0115 0.0009 0.0019 0.0048 0.0009 0.0015 0.0144 0.0009 0.0025 0.001 0.0019 0.0024 0.0044 0.0011 0.0015 0.0045 0.0027 0.0019
Nucleolus 0.0005 0.0001 0.0002 0 0.0001 0.0001 0.0004 0.0002 0.0001 0 0.0005 0.0001 0.0004 0.0011 0.0001 0 0 0.0001
Nucleus 0.0049 0.0015 0.0015 0.0019 0.0009 0.0009 0.0059 0.0017 0.0016 0.001 0.0014 0.0018 0.0022 0.0029 0.0013 0.0013 0.0015 0.0012
Peroxisomes 0.0014 0.0009 0.0085 0.0002 0.0002 0.0002 0.003 0.004 0.0029 0.0001 0.0002 0.0004 0.0021 0.0003 0.0003 0.0001 0.0002 0.0006
Punctate Nuclear 0.0049 0.0005 0.0019 0.0001 0.0002 0.0001 0.012 0.0004 0.0004 0.0001 0.0011 0.0005 0.0025 0.0011 0.0001 0.0001 0.0001 0.001
Vacuole 0.0114 0.0063 0.0082 0.0469 0.0095 0.0051 0.0112 0.0091 0.0136 0.0114 0.005 0.0071 0.0085 0.0055 0.0118 0.0169 0.0066 0.0037
Vacuole Periphery 0.0028 0.0008 0.0029 0.0114 0.0034 0.0027 0.0032 0.0011 0.0044 0.0027 0.0018 0.0025 0.0024 0.0009 0.0031 0.0079 0.0037 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 89.68 125.834 125.001 96.0715 104.4994 113.4561 133.9 99.0299 128.3283 95.9602
Translational Efficiency 3.1207 2.5505 1.9693 3.4175 2.66 2.1997 1.8509 3.3025 2.0824 2.4085

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1627 1621 341 1219 2063 2015 2560 1681 3690 3636 2901 2900

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1266.95 1578.87 2108.55 2168.27 1323.40 1577.37 1930.28 2126.53 1298.51 1578.04 1951.23 2144.08
Standard Deviation 259.40 286.69 279.01 426.96 273.02 259.61 268.05 392.57 268.57 272.02 275.41 407.90
Intensity Change Log 2 — 0.317533 0.734892 0.775185 — 0.253272 0.544561 0.684252 — 0.285060 0.640790 0.729444

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001269 0.001198 0.006355 0.001309 0.000346 0.001139 0.001222 0.000727 0.000753 0.001165 0.001825 0.000972
Bud Neck 0.001515 0.001328 0.013950 0.002534 0.000880 0.001067 0.002442 0.001553 0.001160 0.001183 0.003795 0.001965
Bud Site 0.127965* 0.112814* 0.141694* 0.038770 0.066733 0.116802* 0.080825 0.035615 0.093732 0.115024* 0.087980 0.036941
Cell Periphery 0.001921 0.003608 0.033822 0.017707 0.002028 0.001880 0.002865 0.014333 0.001981 0.002650 0.006504 0.015751
Cytoplasm 0.560247* 0.623347* 0.471138* 0.799862* 0.693285* 0.653200* 0.825845* 0.832081* 0.634626* 0.639891* 0.784151* 0.818538*
Cytoplasmic Foci 0.199031* 0.130930 0.055914 0.008789 0.115433 0.130227 0.018783 0.009626 0.152293 0.130540 0.023148 0.009274
Eisosomes 0.000016 0.000012 0.000036 0.000009 0.000008 0.000012 0.000007 0.000006 0.000012 0.000012 0.000010 0.000007
Endoplasmic Reticulum 0.006767 0.005608 0.007748 0.000794 0.004455 0.004270 0.000690 0.000605 0.005475 0.004867 0.001520 0.000685
Endosome 0.010926 0.010289 0.019651 0.001923 0.008657 0.008844 0.003088 0.001764 0.009657 0.009488 0.005035 0.001831
Golgi 0.047917 0.062539 0.063208 0.010541 0.035152 0.038968 0.007787 0.010573 0.040780 0.049476 0.014301 0.010560
Lipid Particles 0.001650 0.003609 0.063179 0.023683 0.001915 0.002135 0.008260 0.019621 0.001798 0.002792 0.014716 0.021329
Mitochondria 0.001835 0.001518 0.000080 0.000800 0.001793 0.002071 0.000244 0.000142 0.001811 0.001825 0.000225 0.000419
Mitotic Spindle 0.001937 0.003949 0.006972 0.002595 0.001700 0.003856 0.002137 0.000759 0.001804 0.003897 0.002705 0.001530
None 0.000860 0.000341 0.000290 0.000644 0.000736 0.000187 0.000128 0.000283 0.000790 0.000256 0.000147 0.000435
Nuclear Periphery 0.000041 0.000032 0.000227 0.000116 0.000040 0.000024 0.000024 0.000055 0.000040 0.000028 0.000048 0.000080
Nuclear Periphery Foci 0.001115 0.002046 0.012955 0.003538 0.001509 0.000948 0.001058 0.001796 0.001335 0.001438 0.002456 0.002528
Nucleolus 0.000121 0.000029 0.000050 0.000090 0.000088 0.000047 0.000052 0.000082 0.000103 0.000039 0.000052 0.000086
Nucleus 0.001346 0.000696 0.004313 0.004605 0.001630 0.000628 0.002231 0.002692 0.001505 0.000658 0.002476 0.003496
Peroxisomes 0.000688 0.000987 0.000779 0.000348 0.000548 0.000878 0.000852 0.000489 0.000610 0.000927 0.000843 0.000430
Vacuole 0.009799 0.011413 0.085938 0.074257 0.023006 0.011752 0.035892 0.060721 0.017182 0.011601 0.041775 0.066411
Vacuole Periphery 0.023034 0.023706 0.011699 0.007085 0.040057 0.021063 0.005567 0.006477 0.032551 0.022241 0.006288 0.006733

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.34 -7.57 -0.18 -0.41 7.00 -4.29 -10.71 -6.41 2.01 5.09 -3.00 -8.24 -1.56 0.97 5.05
Bud Neck 0.77 -9.81 -4.36 -5.79 8.80 -1.70 -9.73 -5.25 -4.15 4.97 -0.17 -11.83 -6.34 -7.01 7.57
Bud Site 2.43 -1.36 16.54 14.62 10.76 -10.07 -3.80 8.93 17.73* 13.03 -5.42 1.18 18.27 23.05* 15.87
Cell Periphery -4.54 -9.30 -10.66 -9.24 4.34 0.60 -2.44 -10.77 -10.99 -9.99 -3.11 -8.72 -15.15 -14.26 -9.07
Cytoplasm -5.73 4.91 -22.31 -16.24 -17.85 4.33 -16.61 -16.39 -20.29 -1.17 -0.74 -20.78 -27.13 -26.03 -5.37
Cytoplasmic Foci 10.71 22.77 36.91 30.59 12.73 -3.04 28.61 31.63 32.81 9.22 5.48 41.77 47.34 44.85 14.05
Eisosomes 2.47 -6.75 4.34 2.21 9.28 -3.82 1.91 3.20 6.50 1.72 -0.71 1.70 5.49 6.18 4.32
Endoplasmic Reticulum 1.24 -0.50 8.58 7.38 5.33 0.26 7.35 7.42 6.99 0.70 1.05 8.98 11.34 10.18 4.34
Endosome 0.78 -5.01 13.51 13.09 10.70 -0.29 11.18 14.15 14.10 4.66 0.34 9.82 19.55 19.26 9.74
Golgi -4.74 -3.07 17.68 19.66 12.49 -1.89 19.31 14.14 15.42 -2.35 -4.88 20.36 22.32 24.71 3.52
Lipid Particles -4.62 -12.48 -13.64 -12.15 7.47 -0.73 -9.31 -12.81 -12.54 -7.42 -3.93 -14.48 -18.60 -17.38 -4.97
Mitochondria 1.22 10.65 2.47 1.68 -1.66 -0.79 10.34 11.11 6.00 2.69 -0.06 14.11 6.95 5.43 -1.03
Mitotic Spindle -2.92 -5.05 -0.83 1.17 3.52 -2.56 -1.57 2.36 4.07 4.35 -3.76 -3.00 0.51 3.77 3.03
None 2.08 2.29 0.74 -1.65 -1.95 4.93 5.47 3.72 -1.73 -2.84 4.24 5.15 2.45 -2.14 -3.55
Nuclear Periphery 0.72 -9.87 -2.53 -3.01 3.56 2.08 2.25 -1.36 -2.62 -2.58 1.83 -1.80 -2.86 -3.91 -2.22
Nuclear Periphery Foci -2.65 -10.25 -7.17 -4.25 7.83 1.51 1.81 -1.28 -2.60 -4.68 -0.31 -5.24 -6.06 -4.64 -0.67
Nucleolus 2.10 1.61 0.67 -7.63 -5.81 1.32 1.41 0.21 -1.83 -6.47 2.45 2.09 0.68 -4.22 -8.26
Nucleus 1.91 -6.18 -7.88 -12.13 -1.12 5.65 -4.05 -4.15 -7.85 -1.81 4.74 -5.85 -8.47 -13.81 -4.65
Peroxisomes -1.43 -0.78 7.19 3.10 4.14 -2.92 -4.83 1.11 3.37 5.35 -2.83 -4.26 4.81 4.43 7.53
Vacuole -1.40 -11.47 -18.68 -18.44 1.25 7.26 -7.32 -13.41 -18.21 -7.90 5.53 -14.37 -22.51 -25.87 -9.26
Vacuole Periphery -0.35 6.89 10.65 9.91 3.03 9.03 18.76 17.79 11.90 -1.29 7.10 21.80 20.65 15.39 -0.63
Standard name
Human Ortholog
Description Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Tpm2

Tpm2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available