Standard name
Human Ortholog
Description Ribosome-associated, nascent chain binding factor; binds N-terminal region of nascent peptides during translation; recognizes target proteins via its putative metallopeptidase peptide-binding pocket
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.96 0.93 0.95 0.92 0.95 0.86 0.81 0.75 0.72 – 0.58 0.63 0.96 0.97 0.97 0.92 0.92 0.95 0.8 0.78 0.77 0.79 0.82 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.07 0.14 0.08 0.23 0.29 0.35 0.47 – 0.59 0.54 0 0 0 0 0 0 0.05 0 0.1 0 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 2 0 2 3 0 1 – 1 0 1 1 0 3 4 1 0 1 0 2 2 3
Bud 0 0 3 3 6 5 8 16 10 – 10 10 1 1 1 0 0 0 10 0 2 5 11 18
Bud Neck 0 0 1 0 2 1 2 3 0 – 0 1 0 0 0 0 0 0 1 0 2 1 1 1
Bud Site 0 0 0 0 0 1 0 0 1 – 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 0 2 2 1 4 3 1 6 – 11 10 0 1 0 6 14 17 0 0 0 1 0 0
Cytoplasm 189 55 152 175 263 280 388 293 211 – 206 270 176 287 323 368 250 374 165 53 124 155 240 229
Endoplasmic Reticulum 2 1 2 1 0 2 9 3 1 – 6 5 3 5 2 12 7 11 0 0 1 5 7 18
Endosome 0 0 0 0 1 4 5 2 0 – 0 1 0 0 0 8 3 1 4 1 1 2 3 7
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 3 1 0 2 0 0 0 3 8
Mitochondria 5 4 12 27 21 76 140 138 137 – 210 231 3 0 13 10 2 1 10 2 16 6 7 18
Nucleus 0 0 1 1 2 0 2 5 0 – 0 0 0 1 1 3 1 2 0 0 0 0 0 0
Nuclear Periphery 0 4 0 0 1 0 1 0 1 – 2 1 0 1 1 0 0 0 0 0 0 0 0 0
Nucleolus 1 1 0 0 1 0 0 0 0 – 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 1 3 1 4 – 3 3 0 0 0 0 0 0 2 0 3 4 3 7
Vac/Vac Membrane 2 1 0 3 3 1 8 9 1 – 9 13 0 1 1 21 14 18 3 2 2 5 5 7
Unique Cell Count 197 59 160 191 277 324 480 392 294 357 428 183 295 332 401 271 395 206 69 162 197 294 327
Labelled Cell Count 204 66 174 214 302 377 572 471 373 459 546 184 298 344 434 296 425 206 69 162 197 294 327


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 6.0 4.6 4.8 4.8 4.2 4.1 4.2 3.6 – 3.6 3.4 7.1 6.7 5.6 10.1 12.0 11.8 6.4 6.1 7.6
Std Deviation (1e-4) 3.4 3.5 2.1 2.3 2.4 2.1 2.1 1.8 1.8 – 1.6 1.5 4.0 3.7 3.2 1.9 2.4 1.7 3.5 3.3 4.2
Intensity Change (Log2) – – – 0.06 0.06 -0.11 -0.15 -0.14 -0.35 – -0.34 -0.44 0.64 0.55 0.28 1.14 1.39 1.37 0.48 0.43 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 2.1 1.8
Cytoplasm -1.2 0 -2.9 -4.3 -5.5 -5.9 – -8.5 -7.7 0.5 1.3 1.3 -1.3 -1.1 -0.2
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 -2.6 0 -1.7 -2.8 -3.8 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 3.0 2.9 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5793 4.6221 4.6921 3.3002 2.396 3.8149 3.6026 4.7499 4.883 3.7527 4.0409 4.0346 4.1075 6.2068 4.2636 3.9182 4.1574 4.9005
Actin 0.0575 0.0019 0.011 0.001 0.0362 0.0049 0.0257 0.0061 0.017 0.0064 0.0079 0.009 0.0019 0.0026 0.0084 0.0019 0.0055 0.0015
Bud 0.0025 0.0059 0.0037 0.0007 0.0082 0.0022 0.0035 0.007 0.0064 0.0008 0.0018 0.0023 0.0077 0.0172 0.0055 0.0101 0.0012 0.0015
Bud Neck 0.0056 0.0005 0.001 0.0004 0.0022 0.0011 0.001 0.0003 0.0022 0.0003 0.0004 0.0009 0.0002 0.0003 0.0003 0.0007 0.0002 0.0007
Bud Periphery 0.0044 0.0067 0.0068 0.0006 0.017 0.0023 0.0057 0.004 0.0077 0.001 0.002 0.0045 0.0045 0.0132 0.0081 0.0537 0.002 0.0035
Bud Site 0.0051 0.0082 0.0062 0.0003 0.0044 0.0005 0.0102 0.0146 0.0062 0.0003 0.0008 0.0003 0.0027 0.0206 0.0052 0.0012 0.0003 0.0001
Cell Periphery 0.0009 0.0013 0.0005 0.0003 0.0022 0.0002 0.0004 0.0004 0.0003 0.0001 0.0001 0.0001 0.0004 0.0015 0.0007 0.0008 0.0001 0.0001
Cytoplasm 0.5616 0.7259 0.7176 0.614 0.6253 0.7037 0.5618 0.7719 0.6451 0.6165 0.6739 0.704 0.6363 0.6615 0.5983 0.535 0.4927 0.7169
Cytoplasmic Foci 0.019 0.0073 0.0229 0.0115 0.0254 0.0164 0.0357 0.0098 0.0267 0.018 0.047 0.0233 0.0184 0.017 0.0154 0.0449 0.0496 0.0183
Eisosomes 0.0007 0.0001 0.0001 0 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0002 0
Endoplasmic Reticulum 0.0164 0.0197 0.0087 0.0339 0.0087 0.0185 0.0106 0.0021 0.0043 0.0046 0.0074 0.0064 0.0062 0.0036 0.0078 0.0037 0.005 0.0056
Endosome 0.0453 0.0068 0.0211 0.0406 0.0313 0.0267 0.0483 0.0076 0.0441 0.0321 0.0835 0.0305 0.0278 0.0203 0.0225 0.0223 0.0572 0.0373
Golgi 0.0107 0.0009 0.005 0.0013 0.0064 0.0044 0.0143 0.0015 0.0065 0.0177 0.0088 0.0077 0.0038 0.002 0.0027 0.0022 0.0059 0.0029
Lipid Particles 0.0193 0.0006 0.0052 0.0009 0.0065 0.001 0.0099 0.0016 0.0051 0.0049 0.0038 0.0014 0.0009 0.0003 0.0005 0.0003 0.0219 0.0001
Mitochondria 0.0082 0.0006 0.0056 0.001 0.0072 0.0057 0.0173 0.0008 0.011 0.0043 0.0112 0.0052 0.0014 0.0014 0.0095 0.0022 0.0033 0.002
None 0.1692 0.203 0.1622 0.2604 0.1421 0.2019 0.1965 0.164 0.197 0.2783 0.1195 0.1948 0.2775 0.2309 0.3064 0.3137 0.325 0.2041
Nuclear Periphery 0.0313 0.0019 0.0022 0.0142 0.0171 0.001 0.0213 0.0014 0.0019 0.0023 0.0116 0.0014 0.0013 0.0008 0.0016 0.0009 0.002 0.0007
Nucleolus 0.0008 0.0002 0.0005 0.0001 0.0004 0.0001 0.0022 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0.0003 0
Nucleus 0.0127 0.0018 0.0019 0.0035 0.0032 0.001 0.0088 0.0018 0.0026 0.0015 0.0026 0.0014 0.0014 0.0016 0.0015 0.001 0.0009 0.0007
Peroxisomes 0.0091 0.0002 0.0048 0.0003 0.0456 0.0015 0.0092 0.0007 0.006 0.003 0.0025 0.0027 0.0004 0.0003 0.001 0.0006 0.0194 0.0002
Punctate Nuclear 0.0077 0.0008 0.0015 0.0013 0.0019 0.0003 0.0067 0.0012 0.0015 0.0007 0.0028 0.0007 0.0026 0.0006 0.0006 0.0008 0.0027 0.0002
Vacuole 0.0087 0.005 0.0094 0.0114 0.0059 0.0051 0.0078 0.0028 0.0063 0.0054 0.0084 0.0023 0.0037 0.0033 0.0028 0.0032 0.0028 0.0024
Vacuole Periphery 0.0035 0.0007 0.002 0.0023 0.0025 0.0015 0.0028 0.0005 0.0019 0.0016 0.0036 0.001 0.0007 0.0007 0.0007 0.0007 0.0016 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.6553 31.9418 30.143 45.2214 29.4582 33.7709 33.1739 31.8675 36.7939 40.2646
Translational Efficiency 1.0746 1.3373 1.3538 0.8853 1.371 1.4346 1.1845 0.9244 0.9504 0.9027

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
511 1265 97 1121 1591 1837 196 41 2102 3102 293 1162

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 579.33 833.89 1194.52 969.02 672.61 809.65 1158.67 1079.03 649.93 819.54 1170.54 972.90
Standard Deviation 93.11 216.19 246.86 273.93 132.91 208.71 333.76 239.65 130.69 212.13 308.19 273.55
Intensity Change Log 2 — 0.525472 1.043974 0.742141 — 0.267528 0.784628 0.681893 — 0.392641 0.910452 0.710086

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000221 0.000359 0.002031 0.000904 0.000200 0.000403 0.000789 0.000629 0.000205 0.000385 0.001200 0.000895
Bud Neck 0.021154 0.022879 0.003327 0.005277 0.020754 0.025796 0.005551 0.003358 0.020852 0.024606 0.004814 0.005209
Bud Site 0.007886 0.026939 0.036876 0.034095 0.019945 0.043318 0.030075 0.032047 0.017013 0.036639 0.032326 0.034022
Cell Periphery 0.000244 0.000171 0.000440 0.000256 0.000211 0.000257 0.000226 0.000302 0.000219 0.000222 0.000297 0.000258
Cytoplasm 0.490638* 0.538073* 0.558362* 0.579955* 0.496674* 0.573537* 0.356531* 0.448595* 0.495207* 0.559075* 0.423348* 0.575320*
Cytoplasmic Foci 0.233663* 0.201718* 0.099592 0.067733 0.222068* 0.188255* 0.102647 0.186757* 0.224887* 0.193745* 0.101636 0.071933
Eisosomes 0.000281 0.000084 0.000041 0.000025 0.000150 0.000097 0.000058 0.000053 0.000182 0.000091 0.000053 0.000026
Endoplasmic Reticulum 0.000671 0.001202 0.006432 0.001250 0.002949 0.001705 0.003184 0.000663 0.002396 0.001500 0.004259 0.001230
Endosome 0.020789 0.021125 0.033744 0.016172 0.015840 0.022571 0.034083 0.021320 0.017043 0.021981 0.033971 0.016354
Golgi 0.003387 0.005044 0.021104 0.007476 0.004184 0.008292 0.006840 0.008940 0.003990 0.006968 0.011562 0.007527
Lipid Particles 0.010148 0.003720 0.005180 0.003077 0.008052 0.003433 0.007542 0.004445 0.008561 0.003550 0.006760 0.003125
Mitochondria 0.006026 0.004809 0.000155 0.001728 0.005446 0.006325 0.000295 0.000104 0.005587 0.005707 0.000248 0.001671
Mitotic Spindle 0.000591 0.004422 0.009364 0.023413 0.001239 0.009271 0.014019 0.002195 0.001081 0.007294 0.012478 0.022664
None 0.009670 0.003447 0.000496 0.003121 0.004573 0.003289 0.001112 0.000642 0.005812 0.003353 0.000908 0.003034
Nuclear Periphery 0.000392 0.000246 0.000417 0.000691 0.000431 0.000139 0.001198 0.000296 0.000422 0.000183 0.000940 0.000677
Nuclear Periphery Foci 0.000501 0.000827 0.010202 0.002527 0.000340 0.000254 0.006889 0.002492 0.000379 0.000488 0.007986 0.002526
Nucleolus 0.001714 0.001037 0.000183 0.000478 0.001150 0.000878 0.000407 0.000316 0.001287 0.000943 0.000333 0.000473
Nucleus 0.129433 0.079080 0.075515 0.075419 0.128153 0.040647 0.099092 0.075162 0.128464 0.056320 0.091287 0.075410
Peroxisomes 0.005047 0.009164 0.001106 0.002336 0.004851 0.007743 0.001756 0.005958 0.004899 0.008323 0.001541 0.002464
Vacuole 0.056086 0.071297 0.131126 0.169645 0.059265 0.059568 0.323717* 0.203930* 0.058492 0.064351 0.259958* 0.170855
Vacuole Periphery 0.001458 0.004359 0.004308 0.004421 0.003524 0.004222 0.003990 0.001796 0.003022 0.004278 0.004095 0.004328

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.88 -4.37 -2.07 -1.53 3.47 -2.59 -3.20 -1.70 -0.48 0.84 -2.68 -5.39 -3.11 -1.51 2.61
Bud Neck -0.47 6.34 5.79 8.87 -2.59 -2.26 8.93 9.86 12.72 2.39 -2.02 11.06 11.14 15.26 -0.33
Bud Site -8.91 -3.68 -5.52 2.08 1.69 -8.63 -0.45 -0.60 2.14 -0.35 -10.05 -2.37 -1.78 6.14 1.08
Cell Periphery 2.26 -3.25 2.26 0.50 5.29 -0.99 -0.24 -0.16 0.43 -0.05 -0.07 -2.43 2.36 1.88 4.12
Cytoplasm -3.14 -1.03 -0.36 3.23 0.89 -7.83 8.44 2.43 4.07 -1.21 -7.83 5.75 0.35 6.41 -5.22
Cytoplasmic Foci 3.18 10.74 20.82 23.30 3.62 4.67 16.78 3.05 1.85 -2.29 5.32 19.72 32.43 30.09 3.72
Eisosomes 4.70 5.39 5.91 11.22 2.01 3.54 4.54 4.99 3.06 1.58 5.93 7.11 9.80 14.82 5.71
Endoplasmic Reticulum -2.33 -3.95 -4.25 -1.38 3.45 1.44 -1.96 3.17 1.68 7.32 1.47 -3.73 1.72 0.04 6.05
Endosome -0.09 -1.55 5.19 7.11 3.65 -4.34 -2.42 -0.05 1.71 1.52 -3.81 -2.98 6.04 10.14 5.70
Golgi -1.96 -2.45 -0.02 1.57 2.43 -5.41 0.43 -0.47 0.88 -0.59 -5.45 -2.07 0.55 3.55 2.16
Lipid Particles 5.22 3.90 6.48 2.47 3.80 7.43 1.57 4.04 -0.24 2.21 9.00 3.26 10.96 2.89 5.29
Mitochondria 0.51 2.55 2.15 4.24 -2.77 -0.80 6.48 6.78 8.53 3.05 -0.10 6.51 5.27 7.50 -2.41
Mitotic Spindle -3.73 -2.21 -7.33 -5.69 -2.98 -5.40 -2.65 -1.05 4.45 2.35 -6.29 -3.35 -7.16 -4.67 -3.14
None 2.18 3.31 2.46 0.98 -7.14 1.59 4.62 5.20 5.94 1.36 2.64 5.59 3.40 1.47 -5.35
Nuclear Periphery 2.43 -1.83 -10.42 -14.52 -6.51 5.79 -6.32 -0.27 -3.69 5.92 5.75 -6.36 -10.76 -16.02 1.16
Nuclear Periphery Foci -1.54 -5.63 -4.67 -3.05 4.80 2.08 -9.32 -3.01 -3.08 2.14 -1.23 -9.92 -5.54 -4.98 7.30
Nucleolus 2.39 6.41 5.45 4.37 -3.26 1.25 3.67 4.28 4.37 1.31 1.88 5.80 5.34 5.25 -1.00
Nucleus 6.34 2.12 1.14 -5.77 -1.63 19.31 0.01 2.84 -1.05 2.45 17.78 1.16 1.69 -10.67 -0.21
Peroxisomes -2.98 4.02 3.59 7.63 -1.34 -3.39 6.73 0.49 1.73 -1.19 -4.77 8.09 7.40 10.82 -1.03
Vacuole -3.30 -5.87 -22.94 -21.97 -3.99 -0.26 -15.88 -5.73 -5.72 0.74 -2.42 -15.74 -25.27 -24.64 3.00
Vacuole Periphery -4.31 -2.63 -1.40 3.05 1.75 -1.46 2.13 3.63 4.80 1.61 -2.96 0.54 2.06 4.48 1.27
Standard name
Human Ortholog
Description Ribosome-associated, nascent chain binding factor; binds N-terminal region of nascent peptides during translation; recognizes target proteins via its putative metallopeptidase peptide-binding pocket
Localization
Cell Percentages cytoplasm (22%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tma108

Tma108


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tma108-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available