Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, extending into the intermembrane space; interacts with porin (Por1p) and with Om14p; imported via the presequence pathway involving the TOM and TIM23 complexes, then assembled in the outer membrane by Mim1p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 3 | 0 | 0 | 1 | 1 | 0 | 3 | 2 | 0 | 0 | 4 | 4 | 3 | 2 | 6 | 3 | 2 | 7 | 28 | 10 | 6 | 10 | 15 |
Bud | 2 | 8 | 39 | 26 | 19 | 29 | 32 | 22 | 42 | 34 | 28 | 48 | 4 | 3 | 1 | 15 | 8 | 7 | 3 | 8 | 1 | 5 | 8 | 10 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 6 | 1 | 4 | 8 | 8 |
Bud Site | 1 | 2 | 2 | 3 | 6 | 17 | 6 | 5 | 11 | 6 | 9 | 12 | 0 | 0 | 0 | 1 | 5 | 1 | – | – | – | – | – | – |
Cell Periphery | 11 | 27 | 19 | 11 | 9 | 1 | 13 | 4 | 5 | 6 | 10 | 7 | 16 | 15 | 16 | 23 | 6 | 6 | 0 | 1 | 1 | 0 | 1 | 0 |
Cytoplasm | 41 | 73 | 51 | 55 | 36 | 29 | 26 | 20 | 10 | 13 | 12 | 10 | 61 | 56 | 53 | 25 | 38 | 39 | 5 | 9 | 1 | 2 | 3 | 7 |
Endoplasmic Reticulum | 9 | 13 | 6 | 3 | 6 | 6 | 2 | 4 | 1 | 3 | 4 | 7 | 3 | 5 | 4 | 11 | 16 | 11 | 3 | 7 | 3 | 4 | 3 | 4 |
Endosome | 42 | 50 | 16 | 22 | 47 | 3 | 14 | 18 | 1 | 12 | 0 | 5 | 46 | 49 | 81 | 52 | 23 | 27 | 41 | 65 | 49 | 29 | 36 | 42 |
Golgi | 5 | 11 | 1 | 2 | 1 | 0 | 1 | 4 | 1 | 1 | 4 | 5 | 29 | 56 | 84 | 28 | 17 | 4 | 16 | 30 | 14 | 13 | 15 | 18 |
Mitochondria | 269 | 431 | 492 | 503 | 847 | 943 | 1103 | 998 | 825 | 887 | 855 | 1099 | 174 | 208 | 219 | 65 | 23 | 25 | 322 | 493 | 477 | 234 | 256 | 274 |
Nucleus | 2 | 5 | 1 | 2 | 7 | 18 | 19 | 12 | 10 | 6 | 4 | 7 | 6 | 2 | 4 | 5 | 11 | 7 | 0 | 1 | 0 | 1 | 2 | 2 |
Nuclear Periphery | 2 | 3 | 2 | 5 | 39 | 71 | 90 | 65 | 47 | 63 | 37 | 57 | 0 | 4 | 5 | 1 | 3 | 2 | 4 | 4 | 8 | 2 | 4 | 2 |
Nucleolus | 2 | 2 | 2 | 1 | 37 | 87 | 123 | 124 | 57 | 58 | 70 | 85 | 1 | 0 | 4 | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 4 | 1 |
Peroxisomes | 21 | 37 | 21 | 28 | 13 | 9 | 13 | 17 | 5 | 11 | 3 | 10 | 41 | 37 | 86 | 52 | 24 | 19 | 12 | 16 | 24 | 18 | 21 | 18 |
SpindlePole | 11 | 13 | 31 | 21 | 15 | 13 | 15 | 7 | 9 | 10 | 9 | 17 | 14 | 20 | 9 | 43 | 26 | 24 | 4 | 10 | 8 | 3 | 7 | 7 |
Vac/Vac Membrane | 90 | 75 | 19 | 24 | 77 | 81 | 104 | 96 | 56 | 62 | 41 | 35 | 91 | 120 | 140 | 45 | 43 | 37 | 23 | 37 | 7 | 22 | 33 | 34 |
Unique Cell Count | 401 | 609 | 568 | 594 | 957 | 1034 | 1263 | 1163 | 897 | 970 | 896 | 1161 | 395 | 443 | 509 | 289 | 204 | 178 | 457 | 732 | 624 | 354 | 423 | 456 |
Labelled Cell Count | 510 | 753 | 704 | 706 | 1160 | 1309 | 1561 | 1399 | 1082 | 1172 | 1086 | 1408 | 490 | 578 | 708 | 376 | 248 | 211 | 457 | 732 | 624 | 354 | 423 | 456 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.2 | 4.1 | 4.3 | 5.1 | 5.7 | 6.5 | 7.1 | 8.2 | 8.4 | 9.6 | 10.2 | 6.6 | 7.0 | 7.8 | 6.3 | 6.2 | 6.0 | 6.9 | 6.8 | 7.0 |
Std Deviation (1e-4) | 1.2 | 1.2 | 1.1 | 1.4 | 1.4 | 1.7 | 2.4 | 2.6 | 4.0 | 4.7 | 6.0 | 6.3 | 1.6 | 2.0 | 3.4 | 1.4 | 1.6 | 1.3 | 4.9 | 5.5 | 5.8 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.32 | 0.49 | 0.68 | 0.8 | 1.01 | 1.05 | 1.24 | 1.33 | 0.7 | 0.78 | 0.93 | 0.64 | 0.6 | 0.55 | 0.75 | 0.75 | 0.79 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -4.8 | -3.9 | -4.4 | -5.3 | -1.8 | -3.0 | -3.3 | -2.4 | -4.3 | -4.9 | -5.8 | -1.0 | -1.5 | -1.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 2.3 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -3.4 | -5.6 | -3.5 | -5.1 | -4.1 | -4.1 | -3.0 | -4.4 | 0.6 | 0 | -0.2 | 3.0 | -0.3 | 0 |
Cytoplasm | 0.2 | -4.2 | -5.4 | -6.8 | -7.2 | -7.3 | -7.2 | -7.0 | -8.6 | 3.1 | 1.9 | 0.8 | -0.2 | 3.7 | 4.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 5.0 | 0 |
Endosome | 0 | 2.0 | -4.5 | -2.7 | -1.8 | -4.7 | -2.2 | -5.1 | -4.3 | 5.5 | 5.3 | 7.5 | 7.8 | 4.7 | 6.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.3 | 8.5 | 9.9 | 7.3 | 0 | 0 |
Mitochondria | 0 | 1.1 | 2.9 | 0.4 | -0.5 | 3.3 | 3.0 | 6.1 | 5.8 | -14.1 | -13.6 | -15.1 | -18.6 | -19.6 | -18.3 |
Nucleus | 0 | 0 | 2.8 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 4.3 | 6.0 | 6.1 | 5.3 | 5.1 | 5.8 | 4.4 | 4.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 4.2 | 6.7 | 7.4 | 7.8 | 5.7 | 5.5 | 6.4 | 6.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | -3.0 | -4.0 | -3.9 | -3.0 | -4.4 | -3.4 | -4.9 | -4.2 | 4.2 | 3.2 | 7.2 | 7.1 | 4.2 | 3.6 |
SpindlePole | 0 | -4.3 | -4.9 | -5.4 | -6.5 | -5.1 | -5.2 | -5.1 | -4.7 | -1.4 | -0.7 | -3.2 | 4.6 | 3.4 | 3.6 |
Vacuole | 0.6 | 3.7 | 3.6 | 3.9 | 3.9 | 2.5 | 2.6 | 1.2 | -0.4 | 9.4 | 10.9 | 11.2 | 6.4 | 8.0 | 7.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3911 | 1.1335 | 1.3294 | 0.6168 | 1.4579 | 1.3723 | 4.5383 | 4.5686 | 4.3356 | 3.2569 | 4.0783 | 4.7638 | 1.498 | 1.544 | 1.3388 | 1.2203 | 0.8443 | 1.4456 |
Actin | 0.0073 | 0.0153 | 0.0199 | 0.0071 | 0.0222 | 0.0111 | 0.0316 | 0.0076 | 0.0246 | 0.0072 | 0.0228 | 0.0097 | 0.0256 | 0.023 | 0.0099 | 0.0043 | 0.0275 | 0.0051 |
Bud | 0.0033 | 0.0138 | 0.0102 | 0.009 | 0.0044 | 0.0032 | 0.0009 | 0.0027 | 0.007 | 0.0017 | 0.0011 | 0.0008 | 0.0016 | 0.0061 | 0.0039 | 0.0029 | 0.0005 | 0.0017 |
Bud Neck | 0.0016 | 0.0015 | 0.0065 | 0.0098 | 0.0006 | 0.0015 | 0.0026 | 0.0006 | 0.0006 | 0.0033 | 0.0002 | 0.0045 | 0.0022 | 0.0009 | 0.005 | 0.0004 | 0.0049 | 0.0041 |
Bud Periphery | 0.0075 | 0.0225 | 0.011 | 0.0331 | 0.0181 | 0.0044 | 0.0017 | 0.0085 | 0.0204 | 0.0052 | 0.0039 | 0.0023 | 0.0033 | 0.0092 | 0.0149 | 0.0126 | 0.0011 | 0.0049 |
Bud Site | 0.0096 | 0.0453 | 0.0227 | 0.0065 | 0.0026 | 0.0016 | 0.0081 | 0.022 | 0.0146 | 0.0032 | 0.0007 | 0.0009 | 0.0032 | 0.039 | 0.0105 | 0.0009 | 0.001 | 0.001 |
Cell Periphery | 0.0014 | 0.002 | 0.0017 | 0.0027 | 0.0004 | 0.0006 | 0.0012 | 0.0008 | 0.0007 | 0.0005 | 0.0002 | 0.0004 | 0.001 | 0.0011 | 0.0011 | 0.0005 | 0.0004 | 0.0007 |
Cytoplasm | 0.0168 | 0.03 | 0.0194 | 0.01 | 0.0159 | 0.0314 | 0.0184 | 0.0077 | 0.0042 | 0.0022 | 0.0012 | 0.0064 | 0.0172 | 0.026 | 0.0249 | 0.0082 | 0.0055 | 0.0215 |
Cytoplasmic Foci | 0.0632 | 0.0666 | 0.0688 | 0.0399 | 0.091 | 0.0552 | 0.0422 | 0.0334 | 0.0324 | 0.028 | 0.034 | 0.0284 | 0.0397 | 0.0462 | 0.0549 | 0.0488 | 0.0835 | 0.0462 |
Eisosomes | 0.001 | 0.0006 | 0.0009 | 0.0005 | 0.0003 | 0.0004 | 0.0018 | 0.0008 | 0.0006 | 0.0004 | 0.0002 | 0.0004 | 0.0007 | 0.0005 | 0.0007 | 0.0003 | 0.0004 | 0.0004 |
Endoplasmic Reticulum | 0.0031 | 0.0013 | 0.0015 | 0.0009 | 0.0026 | 0.0019 | 0.0039 | 0.0023 | 0.0009 | 0.0005 | 0.0003 | 0.0003 | 0.0028 | 0.0012 | 0.0025 | 0.0008 | 0.0014 | 0.0014 |
Endosome | 0.1148 | 0.0764 | 0.0937 | 0.0846 | 0.1257 | 0.0756 | 0.0942 | 0.0459 | 0.0416 | 0.0167 | 0.0455 | 0.0259 | 0.1168 | 0.0916 | 0.0706 | 0.0368 | 0.0548 | 0.0631 |
Golgi | 0.0489 | 0.0388 | 0.0406 | 0.0375 | 0.0321 | 0.0328 | 0.0528 | 0.0651 | 0.0347 | 0.035 | 0.058 | 0.0382 | 0.0454 | 0.0461 | 0.0377 | 0.0364 | 0.0211 | 0.0384 |
Lipid Particles | 0.0325 | 0.0064 | 0.0204 | 0.0119 | 0.0554 | 0.025 | 0.0319 | 0.0076 | 0.0052 | 0.0037 | 0.0035 | 0.0146 | 0.0428 | 0.0186 | 0.0223 | 0.0371 | 0.0059 | 0.025 |
Mitochondria | 0.512 | 0.5658 | 0.5288 | 0.6394 | 0.4799 | 0.5936 | 0.6279 | 0.7334 | 0.7586 | 0.8387 | 0.7416 | 0.8108 | 0.5614 | 0.576 | 0.6465 | 0.622 | 0.7181 | 0.6871 |
None | 0.0693 | 0.0536 | 0.0597 | 0.0459 | 0.0739 | 0.0754 | 0.0231 | 0.0177 | 0.0045 | 0.0098 | 0.0008 | 0.013 | 0.0656 | 0.0267 | 0.0358 | 0.142 | 0.0043 | 0.034 |
Nuclear Periphery | 0.0125 | 0.0008 | 0.0012 | 0.0009 | 0.0036 | 0.0016 | 0.007 | 0.0013 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0017 | 0.0006 | 0.0024 | 0.0017 | 0.0018 | 0.0014 |
Nucleolus | 0.0055 | 0.0031 | 0.0057 | 0.0015 | 0.0011 | 0.002 | 0.0007 | 0.0006 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0036 | 0.0022 | 0.0008 | 0.0007 | 0.0004 | 0.0009 |
Nucleus | 0.0067 | 0.0025 | 0.0031 | 0.0014 | 0.0025 | 0.0031 | 0.0021 | 0.0005 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0024 | 0.001 | 0.001 | 0.0006 | 0.001 | 0.0011 |
Peroxisomes | 0.0496 | 0.0352 | 0.0635 | 0.0459 | 0.0523 | 0.0579 | 0.0343 | 0.0346 | 0.0368 | 0.0395 | 0.0818 | 0.0397 | 0.0249 | 0.0611 | 0.0347 | 0.0343 | 0.0625 | 0.0386 |
Punctate Nuclear | 0.0096 | 0.0012 | 0.0041 | 0.0017 | 0.0025 | 0.0068 | 0.0032 | 0.0002 | 0.0029 | 0.0005 | 0.0001 | 0.0003 | 0.001 | 0.0003 | 0.0007 | 0.0026 | 0.0009 | 0.0021 |
Vacuole | 0.016 | 0.0121 | 0.012 | 0.0059 | 0.0085 | 0.0095 | 0.0063 | 0.004 | 0.0048 | 0.0014 | 0.0018 | 0.0014 | 0.0271 | 0.0164 | 0.0123 | 0.0031 | 0.0015 | 0.0144 |
Vacuole Periphery | 0.0075 | 0.0053 | 0.0044 | 0.004 | 0.0044 | 0.0054 | 0.0041 | 0.0025 | 0.0032 | 0.0018 | 0.0018 | 0.0015 | 0.0102 | 0.0061 | 0.007 | 0.0031 | 0.0015 | 0.0068 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2.3338 | 0.8345 | 4.3346 | 6.3739 | 7.8672 | 10.2629 | 2.7422 | 11.9591 | 15.9036 | 6.2534 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1882 | 1216 | 1382 | 1551 | 1882 | 1349 | 2221 | 1544 | 3764 | 2565 | 3603 | 3095 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 642.16 | 650.34 | 1014.47 | 1041.40 | 659.52 | 718.63 | 902.54 | 946.15 | 650.84 | 686.26 | 945.47 | 993.88 |
Standard Deviation | 83.48 | 105.67 | 237.10 | 240.49 | 76.51 | 99.54 | 143.79 | 210.99 | 80.54 | 108.01 | 193.06 | 231.21 |
Intensity Change Log 2 | — | 0.018261 | 0.659721 | 0.697520 | — | 0.123833 | 0.452574 | 0.520653 | — | 0.072716 | 0.558483 | 0.610616 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000538 | 0.001433 | 0.001488 | 0.018723 | 0.000455 | 0.001390 | 0.006062 | 0.011062 | 0.000497 | 0.001410 | 0.004307 | 0.014901 |
Bud Neck | 0.026804 | 0.023665 | 0.004937 | 0.005142 | 0.035047 | 0.024121 | 0.012339 | 0.009802 | 0.030926 | 0.023905 | 0.009500 | 0.007467 |
Bud Site | 0.018715 | 0.031212 | 0.018052 | 0.089202 | 0.022580 | 0.024402 | 0.089472 | 0.105829* | 0.020648 | 0.027630 | 0.062078 | 0.097497 |
Cell Periphery | 0.000524 | 0.000856 | 0.000485 | 0.001643 | 0.000281 | 0.000539 | 0.000402 | 0.002421 | 0.000403 | 0.000689 | 0.000434 | 0.002031 |
Cytoplasm | 0.062296 | 0.023186 | 0.005578 | 0.022499 | 0.071123 | 0.033662 | 0.039223 | 0.040201 | 0.066710 | 0.028696 | 0.026318 | 0.031330 |
Cytoplasmic Foci | 0.179131 | 0.085861 | 0.031432 | 0.031157 | 0.216927* | 0.131745 | 0.047371 | 0.047015 | 0.198029* | 0.109992 | 0.041257 | 0.039068 |
Eisosomes | 0.000324 | 0.000155 | 0.000069 | 0.000148 | 0.000182 | 0.000163 | 0.000092 | 0.000165 | 0.000253 | 0.000159 | 0.000083 | 0.000156 |
Endoplasmic Reticulum | 0.001838 | 0.000897 | 0.001203 | 0.001649 | 0.001562 | 0.000874 | 0.002293 | 0.001519 | 0.001700 | 0.000885 | 0.001875 | 0.001584 |
Endosome | 0.067551 | 0.103544 | 0.055497 | 0.036427 | 0.074525 | 0.102396 | 0.044777 | 0.053608 | 0.071038 | 0.102940 | 0.048889 | 0.044998 |
Golgi | 0.085537 | 0.092724 | 0.059835 | 0.103354 | 0.062483 | 0.108054 | 0.062655 | 0.094514 | 0.074010 | 0.100786 | 0.061574 | 0.098944 |
Lipid Particles | 0.025477 | 0.008245 | 0.003413 | 0.000398 | 0.014562 | 0.010474 | 0.000706 | 0.000854 | 0.020019 | 0.009417 | 0.001744 | 0.000625 |
Mitochondria | 0.427557* | 0.497772* | 0.580578* | 0.532311* | 0.364678* | 0.431907* | 0.475424* | 0.484735* | 0.396117* | 0.463132* | 0.515758* | 0.508577* |
Mitotic Spindle | 0.004592 | 0.018141 | 0.044152 | 0.058541 | 0.011072 | 0.019497 | 0.096125 | 0.035143 | 0.007832 | 0.018854 | 0.076190 | 0.046868 |
None | 0.001862 | 0.003676 | 0.001040 | 0.000658 | 0.001020 | 0.001364 | 0.000588 | 0.000665 | 0.001441 | 0.002460 | 0.000761 | 0.000661 |
Nuclear Periphery | 0.000533 | 0.000343 | 0.003714 | 0.000206 | 0.000478 | 0.000349 | 0.000295 | 0.000172 | 0.000506 | 0.000347 | 0.001607 | 0.000189 |
Nuclear Periphery Foci | 0.000238 | 0.000733 | 0.004003 | 0.000335 | 0.000334 | 0.000670 | 0.000313 | 0.000243 | 0.000286 | 0.000700 | 0.001728 | 0.000289 |
Nucleolus | 0.001108 | 0.003615 | 0.001856 | 0.000378 | 0.002263 | 0.002113 | 0.000403 | 0.000287 | 0.001686 | 0.002825 | 0.000960 | 0.000333 |
Nucleus | 0.010673 | 0.006693 | 0.002096 | 0.003184 | 0.016668 | 0.005164 | 0.007363 | 0.002064 | 0.013670 | 0.005889 | 0.005343 | 0.002626 |
Peroxisomes | 0.030073 | 0.044852 | 0.008924 | 0.019545 | 0.036597 | 0.048250 | 0.034462 | 0.036797 | 0.033335 | 0.046639 | 0.024666 | 0.028152 |
Vacuole | 0.034570 | 0.031351 | 0.079580 | 0.042735 | 0.049151 | 0.035057 | 0.056738 | 0.044581 | 0.041861 | 0.033300 | 0.065499 | 0.043656 |
Vacuole Periphery | 0.020059 | 0.021047 | 0.092068 | 0.031766 | 0.018013 | 0.017809 | 0.022897 | 0.028323 | 0.019036 | 0.019344 | 0.049429 | 0.030048 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.07 | -4.61 | -9.47 | -8.95 | -8.92 | -2.97 | -8.14 | -8.79 | -7.77 | -3.59 | -4.67 | -8.80 | -12.67 | -11.71 | -8.70 |
Bud Neck | 1.08 | 11.13 | 10.80 | 8.01 | -0.53 | 3.79 | 10.06 | 11.45 | 6.40 | 2.49 | 3.43 | 14.02 | 15.73 | 10.24 | 2.93 |
Bud Site | -4.12 | 0.08 | -15.03 | -11.62 | -15.05 | -0.81 | -19.15 | -17.19 | -16.20 | -2.91 | -3.66 | -17.19 | -22.77 | -19.72 | -9.24 |
Cell Periphery | -2.00 | 0.42 | -4.72 | -2.80 | -4.76 | -3.49 | -3.04 | -4.15 | -3.62 | -3.92 | -3.27 | -0.73 | -5.75 | -4.56 | -5.64 |
Cytoplasm | 10.13 | 18.09 | 10.59 | -0.34 | -9.02 | 9.05 | 7.52 | 6.80 | -2.02 | -0.02 | 13.37 | 14.68 | 11.98 | -1.69 | -2.27 |
Cytoplasmic Foci | 16.68 | 32.10 | 32.93 | 14.34 | 0.13 | 13.12 | 33.06 | 32.09 | 17.45 | 0.08 | 20.26 | 45.10 | 45.55 | 22.52 | 1.12 |
Eisosomes | 8.16 | 13.05 | 8.70 | 0.65 | -8.84 | 1.61 | 10.59 | 1.48 | -0.15 | -7.63 | 7.85 | 15.87 | 8.28 | 0.35 | -11.24 |
Endoplasmic Reticulum | 3.43 | 2.27 | 0.41 | -4.29 | -2.62 | 2.02 | -1.67 | 0.07 | -3.27 | 2.04 | 3.72 | -0.79 | 0.31 | -5.30 | 1.19 |
Endosome | -7.05 | 3.45 | 10.41 | 14.20 | 6.56 | -5.90 | 9.78 | 5.85 | 10.55 | -3.06 | -9.20 | 9.86 | 11.16 | 17.57 | 1.94 |
Golgi | -1.33 | 5.63 | -3.02 | -1.65 | -7.75 | -9.08 | 0.25 | -6.58 | 2.34 | -6.83 | -7.34 | 4.61 | -6.59 | 0.57 | -10.38 |
Lipid Particles | 8.17 | 11.97 | 14.25 | 7.50 | 5.40 | 2.49 | 13.07 | 12.89 | 7.84 | -0.53 | 7.88 | 17.36 | 18.81 | 10.82 | 4.79 |
Mitochondria | -5.32 | -12.45 | -8.10 | -2.24 | 4.04 | -5.56 | -9.58 | -9.77 | -3.83 | -1.20 | -7.48 | -14.11 | -12.60 | -4.45 | 0.71 |
Mitotic Spindle | -6.00 | -11.71 | -15.12 | -9.79 | -3.10 | -3.61 | -22.02* | -8.98 | -4.97 | 13.64 | -6.83 | -25.10 | -17.41 | -10.77 | 8.39 |
None | -1.93 | 1.57 | 2.27 | 3.59 | 3.26 | -1.20 | 3.67 | 2.13 | 2.39 | -0.34 | -2.13 | 2.53 | 2.84 | 4.22 | 1.17 |
Nuclear Periphery | 0.99 | -5.15 | 2.98 | 0.79 | 5.74 | 0.71 | 1.37 | 3.15 | 1.09 | 3.07 | 1.21 | -4.58 | 4.32 | 1.33 | 6.07 |
Nuclear Periphery Foci | -1.69 | -4.51 | -2.08 | 1.30 | 4.37 | -0.91 | -0.08 | 1.19 | 1.17 | 1.46 | -1.77 | -4.50 | -0.28 | 1.72 | 4.46 |
Nucleolus | -3.39 | -1.75 | 2.87 | 4.26 | 3.56 | 0.27 | 4.29 | 4.76 | 4.03 | 1.58 | -2.46 | 2.62 | 5.54 | 5.76 | 3.72 |
Nucleus | 2.36 | 8.09 | 4.66 | 1.69 | -2.14 | 7.91 | 4.19 | 10.73 | 4.17 | 6.12 | 7.21 | 6.47 | 10.85 | 3.31 | 3.87 |
Peroxisomes | -4.48 | 10.27 | 4.19 | 7.40 | -4.85 | -3.42 | 0.84 | -0.09 | 2.85 | -0.76 | -5.61 | 4.72 | 2.41 | 7.00 | -1.70 |
Vacuole | 1.08 | -12.13 | -3.24 | -4.15 | 8.44 | 4.48 | -3.72 | 0.77 | -3.28 | 4.10 | 4.02 | -10.84 | -1.67 | -5.17 | 8.38 |
Vacuole Periphery | -0.51 | -18.69 | -5.13 | -4.39 | 14.44 | 0.12 | -2.95 | -5.10 | -4.97 | -2.64 | -0.24 | -16.36 | -7.23 | -6.64 | 9.01 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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