Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase complex; catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 4 | 6 | 4 | 0 | 0 | 2 | 3 | 0 | 3 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 5 | 17 | 22 | 18 | 22 | 29 |
Bud | 1 | 7 | 25 | 21 | 26 | 18 | 22 | 17 | 25 | 13 | 15 | 13 | 8 | 3 | 8 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 1 | 1 | 0 | 2 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 1 | 2 | 0 | 3 | 4 | 9 | 4 | 4 | 2 | 5 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 2 | 6 | 15 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 5 | 4 | 1 | 6 | 8 | 7 | 15 | 14 | 7 | 11 | 7 | 5 | 7 | 7 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 5 | 3 | 8 | 2 | 15 | 2 | 2 | 4 | 0 | 0 | 1 | 0 | 1 | 3 | 6 | 1 | 1 | 0 | 1 | 10 | 19 | 7 | 7 | 12 |
Golgi | 14 | 9 | 5 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 3 | 14 | 1 | 7 | 6 | 2 | 12 | 30 | 27 | 25 | 24 | 43 |
Mitochondria | 159 | 284 | 392 | 224 | 247 | 319 | 409 | 479 | 371 | 474 | 309 | 442 | 190 | 198 | 235 | 2 | 2 | 1 | 195 | 232 | 353 | 85 | 113 | 120 |
Nucleus | 1 | 0 | 2 | 0 | 1 | 1 | 2 | 1 | 2 | 0 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 6 | 12 | 12 | 3 | 5 | 3 | 13 | 7 | 5 | 6 | 4 | 9 | 1 | 2 | 5 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 0 |
Nucleolus | 25 | 11 | 22 | 12 | 57 | 62 | 90 | 94 | 65 | 72 | 48 | 58 | 16 | 5 | 8 | 2 | 4 | 7 | 2 | 1 | 1 | 1 | 2 | 4 |
Peroxisomes | 19 | 16 | 65 | 84 | 126 | 39 | 32 | 38 | 14 | 20 | 16 | 15 | 22 | 19 | 22 | 5 | 5 | 3 | 13 | 11 | 53 | 20 | 66 | 45 |
SpindlePole | 3 | 3 | 13 | 12 | 23 | 17 | 12 | 16 | 8 | 2 | 5 | 11 | 3 | 0 | 0 | 1 | 5 | 1 | 0 | 2 | 4 | 1 | 4 | 7 |
Vac/Vac Membrane | 21 | 25 | 26 | 4 | 4 | 4 | 11 | 11 | 6 | 10 | 4 | 7 | 12 | 15 | 16 | 1 | 4 | 2 | 3 | 16 | 4 | 3 | 1 | 1 |
Unique Cell Count | 202 | 313 | 463 | 285 | 401 | 396 | 509 | 590 | 428 | 532 | 355 | 480 | 219 | 226 | 276 | 17 | 19 | 16 | 238 | 333 | 497 | 168 | 253 | 280 |
Labelled Cell Count | 257 | 387 | 599 | 369 | 515 | 480 | 616 | 691 | 515 | 611 | 422 | 567 | 266 | 268 | 314 | 20 | 27 | 18 | 238 | 333 | 497 | 168 | 253 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.0 | 14.7 | 11.3 | 11.4 | 14.5 | 15.2 | 16.4 | 17.5 | 16.9 | 18.6 | 16.9 | 18.7 | 15.2 | 14.6 | 13.5 | 18.1 | 20.4 | 19.3 | 17.2 | 18.5 | 18.9 |
Std Deviation (1e-4) | 4.1 | 3.8 | 3.0 | 2.4 | 5.2 | 4.8 | 5.4 | 5.5 | 5.4 | 6.9 | 6.8 | 7.1 | 4.3 | 3.3 | 3.3 | 4.2 | 7.5 | 6.3 | 4.5 | 5.1 | 5.1 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.36 | 0.42 | 0.53 | 0.63 | 0.58 | 0.72 | 0.58 | 0.73 | 0.43 | 0.36 | 0.26 | 0.68 | 0.85 | 0.77 | 0.6 | 0.71 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1.1 | 0.7 | -0.6 | -0.8 | -2.1 | 0.3 | -2.4 | -0.8 | -2.1 | -1.0 | -2.5 | -1.6 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.7 | -3.3 | -3.2 | -4.1 | -4.4 | -3.4 | -4.2 | -3.0 | -3.6 | -2.2 | 0 | -2.2 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 1.4 | 0.8 | 2.0 | 2.6 | 0.7 | 2.4 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.8 | 0 | 0 | -1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 |
Mitochondria | -2.1 | -7.7 | -1.6 | -1.8 | -1.5 | 0.9 | 2.1 | 1.0 | 3.6 | 0.7 | 1.0 | 0.2 | -7.7 | -8.2 | -8.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.5 | -1.4 | -2.0 | 0 | -1.7 | -1.6 | -1.7 | -1.5 | -0.7 | 0 | 0 | -0.7 | 0 | 0 | 0 |
Nucleolus | -0.3 | 4.8 | 5.4 | 6.3 | 5.8 | 5.2 | 4.7 | 4.4 | 4.0 | 1.4 | -1.6 | -1.2 | 0 | 0 | 0 |
Peroxisomes | 5.1 | 6.1 | -1.9 | -4.0 | -4.1 | -5.6 | -5.8 | -4.5 | -6.0 | -1.5 | -2.1 | -2.5 | 0 | 0 | 0 |
SpindlePole | 1.0 | 2.1 | 1.2 | -0.4 | -0.1 | -0.9 | -3.1 | -1.4 | -0.5 | -1.2 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.9 | -3.7 | -3.7 | -2.8 | -3.3 | -3.4 | -3.1 | -3.4 | -3.5 | -0.1 | 0.5 | 0.1 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 21.84 | 25.9941 | 22.6509 | 20.11 | 22.9125 | 23.4243 | 21.3619 | 23.5881 | 22.7356 | 21.229 | 21.2181 | 21.9038 | 27.0195 | 28.9639 | 25.222 | 26.5163 | 25.6347 | 26.6325 |
Actin | 0.0046 | 0.0096 | 0.0092 | 0.002 | 0.0067 | 0.0073 | 0.0047 | 0.0081 | 0.0043 | 0.0015 | 0.0056 | 0.0038 | 0.0133 | 0.0169 | 0.0074 | 0.003 | 0.0336 | 0.0026 |
Bud | 0.0002 | 0.0002 | 0.0006 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0006 | 0.001 | 0.0016 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0008 | 0.0001 | 0.0045 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Periphery | 0.0002 | 0.0002 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0002 | 0 |
Bud Site | 0.0022 | 0.0056 | 0.0059 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0023 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0028 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 |
Cytoplasm | 0.0001 | 0 | 0.0001 | 0 | 0.001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0687 | 0.1165 | 0.0799 | 0.1122 | 0.0484 | 0.0687 | 0.0992 | 0.1135 | 0.1028 | 0.1112 | 0.0904 | 0.0597 | 0.0606 | 0.0778 | 0.0625 | 0.1405 | 0.1424 | 0.0219 |
Eisosomes | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0004 | 0.0005 | 0.0003 | 0.0004 | 0.0036 | 0.0003 | 0.0014 | 0.0005 | 0.0014 | 0.0002 | 0.0004 | 0.0003 |
Endoplasmic Reticulum | 0.0004 | 0 | 0.0001 | 0 | 0.001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0078 | 0.0052 | 0.0031 | 0.001 | 0.0111 | 0.0024 | 0.0028 | 0.0059 | 0.002 | 0.0072 | 0.0023 | 0.0011 | 0.0005 | 0.0039 | 0.0009 | 0.0006 | 0.0004 | 0.0009 |
Golgi | 0.01 | 0.0109 | 0.0158 | 0.0029 | 0.0037 | 0.016 | 0.0174 | 0.0259 | 0.0164 | 0.0451 | 0.0178 | 0.0089 | 0.0031 | 0.0173 | 0.0096 | 0.002 | 0.0038 | 0.0077 |
Lipid Particles | 0.1532 | 0.1343 | 0.1045 | 0.1141 | 0.1463 | 0.1409 | 0.1702 | 0.1723 | 0.1285 | 0.1047 | 0.2205 | 0.1922 | 0.1004 | 0.0851 | 0.0933 | 0.0641 | 0.1008 | 0.1062 |
Mitochondria | 0.5523 | 0.4667 | 0.5272 | 0.5167 | 0.4614 | 0.56 | 0.5099 | 0.4344 | 0.5593 | 0.5595 | 0.5212 | 0.5748 | 0.6364 | 0.6025 | 0.6437 | 0.5589 | 0.4947 | 0.7 |
None | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.0031 | 0.0001 | 0.0001 | 0 | 0.0094 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.0058 | 0.0038 | 0.0026 | 0.0013 | 0.0008 | 0.0052 | 0.0065 | 0.0046 | 0.0046 | 0.0046 | 0.0069 | 0.0086 | 0.0016 | 0.0008 | 0.0015 | 0.0007 | 0.0303 | 0.0012 |
Nucleus | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0019 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 |
Peroxisomes | 0.181 | 0.2323 | 0.238 | 0.2469 | 0.2755 | 0.1895 | 0.1802 | 0.2114 | 0.1764 | 0.137 | 0.1251 | 0.145 | 0.1747 | 0.1874 | 0.1776 | 0.2287 | 0.1911 | 0.1483 |
Punctate Nuclear | 0.0005 | 0.0001 | 0.0014 | 0.0001 | 0.0025 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0044 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 |
Vacuole | 0.0022 | 0.0027 | 0.0022 | 0.0003 | 0.0067 | 0.0009 | 0.001 | 0.0027 | 0.0006 | 0.0033 | 0.0006 | 0.0005 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0012 |
Vacuole Periphery | 0.0059 | 0.0099 | 0.0061 | 0.0017 | 0.0217 | 0.0069 | 0.0062 | 0.016 | 0.0042 | 0.0194 | 0.0047 | 0.0043 | 0.0016 | 0.0032 | 0.0014 | 0.0006 | 0.0009 | 0.0092 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 68.6684 | 65.7584 | 66.1024 | 76.9935 | 66.2451 | 56.1356 | 71.2374 | 68.4179 | 69.0617 | 72.5527 |
Translational Efficiency | 1.0532 | 1.0136 | 0.9616 | 0.8615 | 0.9387 | 1.3532 | 0.9089 | 0.8338 | 0.9474 | 0.9226 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1735 | 1315 | 185 | 782 | 2034 | 826 | 452 | 2112 | 3769 | 2141 | 637 | 2894 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 982.95 | 1189.98 | 3500.76 | 2001.41 | 1207.37 | 1312.20 | 2164.15 | 2452.72 | 1104.06 | 1237.13 | 2552.33 | 2330.77 |
Standard Deviation | 222.02 | 250.85 | 788.51 | 455.48 | 232.80 | 245.33 | 455.43 | 567.60 | 253.87 | 255.75 | 834.22 | 575.62 |
Intensity Change Log 2 | — | 0.275747 | 1.832478 | 1.025827 | — | 0.120120 | 0.841933 | 1.022515 | — | 0.192044 | 1.370911 | 1.024002 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.008531 | 0.011638 | 0.015942 | 0.024026 | 0.001922 | 0.005375 | 0.013230 | 0.001187 | 0.004964 | 0.009222 | 0.014017 | 0.007359 |
Bud Neck | 0.005852 | 0.002915 | 0.020695 | 0.005989 | 0.000547 | 0.001162 | 0.011255 | 0.000087 | 0.002989 | 0.002239 | 0.013996 | 0.001682 |
Bud Site | 0.011516 | 0.003833 | 0.009118 | 0.006352 | 0.000362 | 0.001177 | 0.007134 | 0.000174 | 0.005496 | 0.002809 | 0.007710 | 0.001844 |
Cell Periphery | 0.000938 | 0.000164 | 0.000255 | 0.000148 | 0.000030 | 0.000104 | 0.000149 | 0.000158 | 0.000448 | 0.000141 | 0.000180 | 0.000155 |
Cytoplasm | 0.002716 | 0.001012 | 0.001002 | 0.001878 | 0.000239 | 0.000363 | 0.001075 | 0.000067 | 0.001379 | 0.000762 | 0.001054 | 0.000556 |
Cytoplasmic Foci | 0.026693 | 0.029022 | 0.210170* | 0.094061 | 0.015301 | 0.032616 | 0.106963 | 0.000879 | 0.020545 | 0.030409 | 0.136937 | 0.026058 |
Eisosomes | 0.000652 | 0.001413 | 0.004411 | 0.002784 | 0.000656 | 0.000780 | 0.002015 | 0.000556 | 0.000654 | 0.001169 | 0.002711 | 0.001158 |
Endoplasmic Reticulum | 0.000607 | 0.000341 | 0.000278 | 0.000255 | 0.000074 | 0.000281 | 0.000244 | 0.000018 | 0.000319 | 0.000317 | 0.000254 | 0.000082 |
Endosome | 0.015082 | 0.008821 | 0.003426 | 0.004864 | 0.001607 | 0.006652 | 0.004368 | 0.000225 | 0.007810 | 0.007984 | 0.004095 | 0.001478 |
Golgi | 0.021987 | 0.033052 | 0.029388 | 0.055528 | 0.018566 | 0.049743 | 0.029386 | 0.003074 | 0.020141 | 0.039491 | 0.029386 | 0.017248 |
Lipid Particles | 0.016099 | 0.013589 | 0.035143 | 0.025157 | 0.008040 | 0.012616 | 0.020292 | 0.000302 | 0.011750 | 0.013214 | 0.024605 | 0.007018 |
Mitochondria | 0.818729* | 0.830210* | 0.432044* | 0.580471* | 0.928005* | 0.860530* | 0.658161* | 0.988125* | 0.877702* | 0.841907* | 0.592491* | 0.877971* |
Mitotic Spindle | 0.002090 | 0.002502 | 0.073766 | 0.011174 | 0.001032 | 0.001277 | 0.028272 | 0.000042 | 0.001519 | 0.002029 | 0.041485 | 0.003050 |
None | 0.004043 | 0.001330 | 0.003489 | 0.001692 | 0.001415 | 0.001446 | 0.002578 | 0.001100 | 0.002625 | 0.001375 | 0.002843 | 0.001260 |
Nuclear Periphery | 0.000241 | 0.000149 | 0.005956 | 0.000645 | 0.000150 | 0.000239 | 0.002932 | 0.000038 | 0.000192 | 0.000184 | 0.003811 | 0.000202 |
Nuclear Periphery Foci | 0.000945 | 0.000531 | 0.002339 | 0.000406 | 0.000154 | 0.000303 | 0.001749 | 0.000011 | 0.000518 | 0.000443 | 0.001921 | 0.000117 |
Nucleolus | 0.002286 | 0.000978 | 0.011541 | 0.001820 | 0.000322 | 0.000687 | 0.003927 | 0.000030 | 0.001226 | 0.000866 | 0.006138 | 0.000513 |
Nucleus | 0.000892 | 0.000198 | 0.000534 | 0.000166 | 0.000041 | 0.000432 | 0.000350 | 0.000006 | 0.000432 | 0.000288 | 0.000403 | 0.000049 |
Peroxisomes | 0.041363 | 0.051517 | 0.095773 | 0.171610* | 0.017862 | 0.016517 | 0.072137 | 0.001950 | 0.028680 | 0.038014 | 0.079001 | 0.047795 |
Vacuole | 0.011780 | 0.004466 | 0.025937 | 0.005794 | 0.001832 | 0.003899 | 0.016618 | 0.000492 | 0.006411 | 0.004247 | 0.019325 | 0.001925 |
Vacuole Periphery | 0.006958 | 0.002320 | 0.018791 | 0.005180 | 0.001843 | 0.003800 | 0.017165 | 0.001479 | 0.004198 | 0.002891 | 0.017637 | 0.002479 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.68 | -2.48 | -5.11 | -4.01 | -2.09 | -4.13 | -5.35 | 1.69 | 4.89 | 5.68 | -3.98 | -5.08 | -2.38 | 1.55 | 3.57 |
Bud Neck | 4.01 | -7.35 | -0.19 | -5.19 | 7.44 | -3.40 | -8.41 | 5.26 | 6.65 | 8.79 | 1.99 | -9.89 | 3.92 | 2.08 | 11.39 |
Bud Site | 4.51 | 1.17 | 2.96 | -2.26 | 1.69 | -1.68 | -5.50 | 1.62 | 2.07 | 5.65 | 3.08 | -1.89 | 4.90 | 1.68 | 5.92 |
Cell Periphery | 4.99 | 4.09 | 5.09 | 0.60 | 1.58 | -4.14 | -8.54 | -3.66 | -1.37 | -0.21 | 4.24 | 3.60 | 3.87 | -0.49 | 0.75 |
Cytoplasm | 3.06 | 3.36 | 1.64 | -3.17 | -5.71 | -2.21 | -8.87 | 3.24 | 5.83 | 11.04 | 2.24 | 1.37 | 3.46 | 1.32 | 6.28 |
Cytoplasmic Foci | -0.85 | -11.41 | -11.83 | -11.28 | 6.88 | -5.71 | -11.77 | 12.57 | 11.22 | 13.74 | -5.13 | -15.58 | -2.84 | 1.84 | 14.62 |
Eisosomes | -5.90 | -6.38 | -8.39 | -5.00 | 2.55 | -1.10 | -6.44 | 0.92 | 2.17 | 7.08 | -5.52 | -8.99 | -5.00 | 0.10 | 6.50 |
Endoplasmic Reticulum | 2.52 | 3.59 | 4.16 | 1.16 | 0.40 | -3.69 | -5.20 | 4.43 | 4.79 | 7.43 | -0.01 | 1.46 | 6.21 | 4.93 | 6.41 |
Endosome | 3.67 | 8.33 | 7.34 | 2.95 | -1.60 | -4.40 | -3.03 | 6.57 | 5.68 | 4.63 | -0.17 | 4.18 | 10.23 | 7.53 | 3.82 |
Golgi | -3.79 | -1.58 | -7.51 | -4.65 | -4.31 | -7.30 | -3.04 | 8.91 | 11.67 | 8.18 | -7.96 | -3.27 | 1.69 | 8.90 | 4.21 |
Lipid Particles | 1.18 | -3.15 | -3.05 | -3.92 | 1.56 | -2.37 | -4.73 | 7.47 | 7.53 | 8.42 | -1.02 | -5.01 | 4.11 | 4.67 | 7.02 |
Mitochondria | -1.13 | 14.93 | 15.35 | 15.87 | -5.22 | 7.11 | 15.96 | -13.85 | -14.72 | -20.02* | 5.09 | 19.21 | -0.05 | -4.70 | -18.82 |
Mitotic Spindle | -0.48 | -6.17 | -6.40 | -5.79 | 5.36 | -0.45 | -6.29 | 2.48 | 3.11 | 6.54 | -0.93 | -8.63 | -3.13 | -1.73 | 8.30 |
None | 3.02 | 0.61 | 2.62 | -2.97 | 4.70 | -0.25 | -6.74 | 2.77 | 2.71 | 8.51 | 2.98 | -0.44 | 3.25 | 1.31 | 9.48 |
Nuclear Periphery | 3.35 | -8.08 | -6.30 | -8.14 | 7.48 | -2.86 | -5.20 | 5.95 | 6.96 | 5.41 | 0.43 | -8.32 | -0.46 | -0.83 | 8.29 |
Nuclear Periphery Foci | 1.33 | -3.47 | 2.87 | 0.49 | 5.42 | -2.20 | -3.24 | 5.72 | 4.48 | 3.54 | 0.41 | -3.74 | 4.63 | 2.09 | 4.95 |
Nucleolus | 2.85 | -4.54 | 0.88 | -2.07 | 4.79 | -1.07 | -6.18 | 3.00 | 1.92 | 6.77 | 1.26 | -6.57 | 3.33 | 1.69 | 7.73 |
Nucleus | 3.73 | 1.87 | 4.03 | 0.57 | 4.92 | -1.17 | -6.02 | 2.56 | 1.28 | 6.95 | 0.89 | 0.30 | 4.62 | 1.80 | 8.56 |
Peroxisomes | -2.18 | -4.30 | -12.92 | -11.63 | -4.85 | 0.45 | -7.59 | 8.45 | 6.36 | 10.10 | -3.19 | -7.98 | -5.58 | -2.53 | 4.61 |
Vacuole | 5.56 | -3.79 | 4.36 | -1.23 | 5.48 | -2.91 | -6.62 | 3.79 | 5.15 | 7.27 | 2.91 | -6.58 | 7.53 | 4.05 | 9.13 |
Vacuole Periphery | 7.17 | -3.86 | 2.02 | -4.11 | 4.42 | -4.10 | -5.75 | 1.21 | 4.72 | 5.88 | 3.47 | -6.41 | 4.50 | 1.21 | 7.25 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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