Standard name | |
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Human Ortholog | |
Description | DNA-binding transcriptional activator; involved in cell cycle regulation; overexpression promotes recovery from pheromone induced arrest via CLN1/2 transcription, induction of of IME1 during sporulation, and suppression of stress sensitivity resulting from mutation of the E3 ubiquitin ligase Rsp5p; binds upstream of BAR1 and cell cycle-related genes; phosphorylated form may be ubiquitinated by Dma2p; potential Cdc28p substrate; regulated by Swi4/6 cell-cycle box binding factor (SBF) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 1 | 0 | 5 | 13 | 4 | 9 | 2 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 9 | 2 | 5 | 13 | 9 |
Bud Neck | 0 | 0 | 3 | 1 | 1 | 0 | 1 | 6 | 1 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 4 | 10 | 9 | 9 | 12 | 11 | 6 | 0 | 0 | 0 | 0 | 2 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 31 | 20 | 19 | 25 | 61 | 43 | 66 | 43 | 37 | 37 | 22 | 23 | 12 | 28 | 42 | 11 | 11 | 9 | 3 | 7 | 10 | 8 | 10 | 9 |
Endoplasmic Reticulum | 0 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 6 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 1 | 7 | 1 | 2 | 8 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 4 | 8 | 4 | 3 | 3 | 1 | 2 | 5 | 9 |
Golgi | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 1 | 1 | 1 |
Mitochondria | 99 | 13 | 7 | 14 | 24 | 77 | 174 | 133 | 194 | 163 | 165 | 138 | 3 | 0 | 1 | 6 | 11 | 6 | 2 | 2 | 6 | 2 | 6 | 4 |
Nucleus | 158 | 131 | 61 | 62 | 109 | 79 | 127 | 125 | 132 | 124 | 104 | 161 | 59 | 104 | 101 | 80 | 87 | 31 | 172 | 140 | 69 | 70 | 130 | 122 |
Nuclear Periphery | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 2 | 0 | 1 | 2 |
Nucleolus | 0 | 4 | 0 | 1 | 0 | 0 | 3 | 1 | 5 | 6 | 6 | 5 | 0 | 0 | 0 | 0 | 3 | 1 | 10 | 11 | 2 | 16 | 18 | 20 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 3 | 4 | 9 | 6 | 7 | 10 | 18 | 7 | 9 | 2 | 10 | 1 | 3 | 3 | 2 | 1 | 0 | 30 | 11 | 9 | 11 | 16 | 24 |
Vac/Vac Membrane | 15 | 28 | 2 | 3 | 13 | 8 | 10 | 8 | 11 | 9 | 2 | 8 | 0 | 6 | 3 | 17 | 24 | 17 | 37 | 34 | 22 | 17 | 30 | 39 |
Unique Cell Count | 250 | 189 | 90 | 103 | 201 | 180 | 295 | 270 | 282 | 270 | 211 | 265 | 74 | 136 | 153 | 108 | 135 | 58 | 285 | 226 | 134 | 142 | 246 | 252 |
Labelled Cell Count | 307 | 214 | 98 | 119 | 223 | 221 | 411 | 359 | 403 | 373 | 315 | 357 | 75 | 142 | 158 | 125 | 151 | 74 | 285 | 226 | 134 | 142 | 246 | 252 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 5.4 | 5.2 | 4.8 | 4.7 | 4.2 | 3.9 | 4.3 | 3.5 | 3.7 | 3.2 | 3.9 | 5.0 | 5.4 | 5.5 | 7.4 | 6.5 | 6.1 | 5.5 | 5.8 | 6.3 |
Std Deviation (1e-4) | 0.7 | 1.4 | 1.1 | 1.6 | 1.3 | 1.8 | 2.0 | 1.7 | 1.2 | 1.0 | 0.6 | 0.9 | 0.9 | 1.3 | 1.5 | 1.6 | 1.5 | 1.3 | 2.0 | 1.9 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.12 | -0.3 | -0.4 | -0.26 | -0.56 | -0.46 | -0.68 | -0.4 | -0.06 | 0.07 | 0.09 | 0.51 | 0.34 | 0.23 | 0.11 | 0.17 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | 1.6 | 0.5 | 0.3 | -1.1 | -1.8 | -1.7 | -2.5 | -3.2 | -0.8 | -0.1 | 1.1 | -2.1 | -2.8 | -0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | 0.1 | 0.5 |
Nucleus | -1.1 | -2.2 | -3.7 | -4.1 | -3.5 | -3.5 | -3.6 | -3.0 | -1.2 | 1.7 | 1.4 | -0.3 | 1.0 | -0.5 | -1.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.6 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8374 | 1.398 | 1.2226 | 0.8 | 0.6637 | 1.1725 | -0.4545 | 0.9482 | 0.1559 | 0.1153 | -0.0395 | 0.5079 | -0.3125 | 0.4774 | 0.2654 | 0.0595 | -0.3542 | 0.0306 |
Actin | 0.0179 | 0.0001 | 0.0087 | 0.0004 | 0.0318 | 0.0008 | 0.0038 | 0 | 0 | 0.0001 | 0.0015 | 0.0019 | 0.0097 | 0.0004 | 0.0067 | 0.027 | 0.0064 | 0.0026 |
Bud | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0007 | 0.0003 | 0.0001 | 0.0001 |
Bud Neck | 0.009 | 0.0003 | 0.0003 | 0.0034 | 0.0023 | 0.0032 | 0.0008 | 0.0002 | 0.0002 | 0.0004 | 0.001 | 0.002 | 0.0019 | 0.0003 | 0.003 | 0.0007 | 0.0005 | 0.0023 |
Bud Periphery | 0.0007 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0009 | 0 | 0.0013 | 0.0006 | 0.0004 | 0.0002 |
Bud Site | 0.006 | 0.0005 | 0.001 | 0.0007 | 0.003 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0019 | 0.0003 | 0.0025 | 0.0009 | 0.0003 | 0.0001 |
Cell Periphery | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0006 | 0.0002 | 0 | 0 |
Cytoplasm | 0.0214 | 0.0151 | 0.0062 | 0.0082 | 0.0083 | 0.0039 | 0.0131 | 0.0028 | 0.0041 | 0.0048 | 0.0039 | 0.0017 | 0.02 | 0.0139 | 0.0141 | 0.0148 | 0.0032 | 0.0029 |
Cytoplasmic Foci | 0.0163 | 0.0015 | 0.0081 | 0.0012 | 0.0055 | 0.0012 | 0.0046 | 0.0003 | 0.0004 | 0.0001 | 0.0039 | 0.0001 | 0.0097 | 0.0009 | 0.0045 | 0.0033 | 0.018 | 0.0011 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0029 | 0.0009 | 0.0028 | 0.0008 | 0.0009 | 0.0006 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0034 | 0.0004 | 0.0007 | 0.0012 | 0.0006 | 0.0005 |
Endosome | 0.0191 | 0.0016 | 0.0143 | 0.0049 | 0.0114 | 0.0016 | 0.0024 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0141 | 0.0004 | 0.016 | 0.0111 | 0.0059 | 0.0027 |
Golgi | 0.0054 | 0.0001 | 0.0042 | 0.0016 | 0.006 | 0.0002 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0047 | 0 | 0.0081 | 0.0114 | 0.0073 | 0.001 |
Lipid Particles | 0.0115 | 0.0005 | 0.0058 | 0.0153 | 0.025 | 0.001 | 0.0011 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0229 | 0.0001 | 0.0034 | 0.0114 | 0.0276 | 0.0068 |
Mitochondria | 0.0089 | 0.0003 | 0.0105 | 0.0041 | 0.002 | 0.0053 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0058 | 0.0002 | 0.0078 | 0.045 | 0.0046 | 0.0012 |
None | 0.029 | 0.0431 | 0.012 | 0.0048 | 0.0332 | 0.0079 | 0.0332 | 0.014 | 0.003 | 0.0485 | 0.0376 | 0.0072 | 0.0242 | 0.0074 | 0.0211 | 0.0391 | 0.0015 | 0.0119 |
Nuclear Periphery | 0.0266 | 0.0136 | 0.0161 | 0.0172 | 0.0198 | 0.0078 | 0.0126 | 0.0111 | 0.0063 | 0.0068 | 0.0103 | 0.0061 | 0.0252 | 0.0099 | 0.0119 | 0.0053 | 0.0275 | 0.0071 |
Nucleolus | 0.0164 | 0.0116 | 0.0087 | 0.0134 | 0.0287 | 0.0538 | 0.0275 | 0.0184 | 0.012 | 0.0105 | 0.0411 | 0.0428 | 0.0202 | 0.0125 | 0.0146 | 0.0081 | 0.0168 | 0.043 |
Nucleus | 0.738 | 0.8883 | 0.8703 | 0.8687 | 0.7556 | 0.8783 | 0.8493 | 0.9106 | 0.9363 | 0.8836 | 0.7191 | 0.9112 | 0.7618 | 0.9345 | 0.8511 | 0.7933 | 0.8536 | 0.8997 |
Peroxisomes | 0.0104 | 0.0001 | 0.0079 | 0.0011 | 0.0037 | 0.0027 | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0.0129 | 0.0001 | 0.0019 | 0.0022 | 0.0085 | 0.0013 |
Punctate Nuclear | 0.0531 | 0.021 | 0.0197 | 0.0486 | 0.0595 | 0.0308 | 0.0469 | 0.0414 | 0.0371 | 0.0445 | 0.1802 | 0.0258 | 0.0528 | 0.0182 | 0.0263 | 0.0225 | 0.015 | 0.0143 |
Vacuole | 0.0037 | 0.0011 | 0.0013 | 0.0035 | 0.0018 | 0.0003 | 0.0012 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0051 | 0.0003 | 0.0023 | 0.0007 | 0.0006 | 0.0006 |
Vacuole Periphery | 0.0022 | 0.0003 | 0.0016 | 0.0011 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0018 | 0.0001 | 0.0013 | 0.0006 | 0.0013 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.6571 | 2.8022 | 4.8518 | 10.0915 | 4.7416 | 3.1329 | 3.7777 | 11.4737 | 7.4865 | 4.5552 |
Translational Efficiency | 0.6886 | 1.7976 | 2.1476 | 0.591 | 0.4909 | 0.7608 | 1.4733 | 0.7565 | 0.9801 | 0.7385 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1839 | 1466 | 2397 | 1527 | 1011 | 2006 | 1601 | 1829 | 2850 | 3472 | 3998 | 3356 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 726.98 | 666.09 | 816.01 | 884.96 | 700.12 | 756.11 | 872.93 | 923.30 | 717.45 | 718.10 | 838.80 | 905.86 |
Standard Deviation | 84.39 | 110.04 | 102.14 | 131.99 | 81.16 | 94.59 | 103.87 | 135.62 | 84.24 | 110.72 | 106.56 | 135.33 |
Intensity Change Log 2 | — | -0.126199 | 0.166671 | 0.283697 | — | 0.110994 | 0.318264 | 0.399197 | — | -0.004962 | 0.243032 | 0.341516 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000103 | 0.000603 | 0.000484 | 0.001215 | 0.000108 | 0.000327 | 0.000460 | 0.000908 | 0.000104 | 0.000444 | 0.000474 | 0.001048 |
Bud Neck | 0.003096 | 0.010754 | 0.024151 | 0.026689 | 0.002064 | 0.009967 | 0.007339 | 0.027930 | 0.002730 | 0.010299 | 0.017418 | 0.027365 |
Bud Site | 0.000906 | 0.004961 | 0.008658 | 0.010355 | 0.000351 | 0.004224 | 0.001129 | 0.013160 | 0.000709 | 0.004535 | 0.005643 | 0.011884 |
Cell Periphery | 0.000136 | 0.000396 | 0.000169 | 0.000169 | 0.000120 | 0.000184 | 0.000107 | 0.000198 | 0.000130 | 0.000273 | 0.000144 | 0.000185 |
Cytoplasm | 0.067388 | 0.081806 | 0.044426 | 0.013697 | 0.050942 | 0.041712 | 0.012739 | 0.026020 | 0.061554 | 0.058641 | 0.031737 | 0.020413 |
Cytoplasmic Foci | 0.011462 | 0.015610 | 0.000742 | 0.003347 | 0.007732 | 0.007346 | 0.000890 | 0.005464 | 0.010138 | 0.010836 | 0.000801 | 0.004501 |
Eisosomes | 0.000027 | 0.000041 | 0.000024 | 0.000030 | 0.000028 | 0.000029 | 0.000023 | 0.000030 | 0.000027 | 0.000034 | 0.000024 | 0.000030 |
Endoplasmic Reticulum | 0.001564 | 0.004715 | 0.004123 | 0.009618 | 0.001525 | 0.003498 | 0.003841 | 0.007085 | 0.001550 | 0.004012 | 0.004010 | 0.008238 |
Endosome | 0.000810 | 0.002320 | 0.001132 | 0.006866 | 0.000611 | 0.002137 | 0.000634 | 0.007159 | 0.000739 | 0.002214 | 0.000933 | 0.007025 |
Golgi | 0.000414 | 0.001589 | 0.000183 | 0.003630 | 0.000644 | 0.001010 | 0.000101 | 0.004508 | 0.000496 | 0.001254 | 0.000150 | 0.004109 |
Lipid Particles | 0.003159 | 0.002979 | 0.000343 | 0.000567 | 0.002496 | 0.001947 | 0.000397 | 0.000588 | 0.002924 | 0.002383 | 0.000365 | 0.000578 |
Mitochondria | 0.003076 | 0.004412 | 0.003081 | 0.012174 | 0.001259 | 0.007553 | 0.001108 | 0.009072 | 0.002431 | 0.006227 | 0.002291 | 0.010483 |
Mitotic Spindle | 0.003373 | 0.004705 | 0.011413 | 0.044805 | 0.001256 | 0.006531 | 0.002370 | 0.060456 | 0.002622 | 0.005760 | 0.007792 | 0.053335 |
None | 0.013159 | 0.046124 | 0.007003 | 0.004958 | 0.011027 | 0.011373 | 0.009283 | 0.013628 | 0.012403 | 0.026046 | 0.007916 | 0.009683 |
Nuclear Periphery | 0.001936 | 0.001798 | 0.002222 | 0.006053 | 0.001288 | 0.002317 | 0.000974 | 0.004161 | 0.001706 | 0.002098 | 0.001723 | 0.005022 |
Nuclear Periphery Foci | 0.000716 | 0.001871 | 0.000433 | 0.002675 | 0.000683 | 0.001137 | 0.000222 | 0.001009 | 0.000704 | 0.001447 | 0.000348 | 0.001767 |
Nucleolus | 0.010428 | 0.023511 | 0.007210 | 0.005697 | 0.011910 | 0.015085 | 0.005983 | 0.007396 | 0.010954 | 0.018643 | 0.006719 | 0.006623 |
Nucleus | 0.875630* | 0.783952* | 0.878271* | 0.823642* | 0.903792* | 0.876389* | 0.949697* | 0.793373* | 0.885620* | 0.837359* | 0.906873* | 0.807146* |
Peroxisomes | 0.001009 | 0.003014 | 0.000272 | 0.000354 | 0.000824 | 0.001988 | 0.000194 | 0.000873 | 0.000943 | 0.002422 | 0.000241 | 0.000637 |
Vacuole | 0.001432 | 0.004088 | 0.005149 | 0.016217 | 0.001193 | 0.004468 | 0.002378 | 0.013756 | 0.001347 | 0.004308 | 0.004039 | 0.014876 |
Vacuole Periphery | 0.000178 | 0.000749 | 0.000512 | 0.007240 | 0.000147 | 0.000778 | 0.000130 | 0.003227 | 0.000167 | 0.000766 | 0.000359 | 0.005053 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.71 | -15.85 | -14.51 | -6.54 | -8.31 | -7.13 | -11.01 | -19.37 | -13.74 | -8.64 | -11.22 | -19.06 | -23.39 | -13.63 | -12.19 |
Bud Neck | -7.39 | -18.62 | -16.70 | -10.13 | -0.56 | -7.98 | -10.85 | -20.31 | -12.32 | -13.88 | -10.27 | -20.67 | -25.80 | -15.94 | -7.22 |
Bud Site | -5.49 | -7.19 | -6.53 | -2.38 | 0.27 | -5.61 | -5.53 | -8.60 | -3.83 | -7.23 | -7.37 | -7.78 | -10.48 | -4.46 | -2.91 |
Cell Periphery | -6.27 | -0.91 | -0.08 | 6.38 | 1.04 | -2.83 | 1.59 | -1.38 | 2.05 | -3.62 | -6.58 | 0.14 | -0.85 | 6.45 | -1.23 |
Cytoplasm | -2.77 | 7.74 | 17.03 | 16.98 | 12.22 | 2.40 | 10.98 | 8.16 | 8.03 | -5.69 | 1.10 | 12.68 | 17.93 | 18.06 | 7.16 |
Cytoplasmic Foci | -2.11 | 10.50 | 9.19 | 8.71 | -1.35 | 0.83 | 7.44 | 5.81 | 5.94 | -2.36 | -0.34 | 12.78 | 10.70 | 10.54 | -2.78 |
Eisosomes | -6.03 | 0.09 | -4.63 | 2.60 | -4.97 | 0.20 | 1.44 | -2.93 | -3.89 | -5.59 | -4.07 | 1.01 | -5.68 | -0.87 | -7.48 |
Endoplasmic Reticulum | -11.42 | -14.41 | -16.72 | -10.09 | -10.00 | -8.74 | -14.22 | -16.76 | -11.06 | -8.74 | -13.76 | -19.59 | -23.49 | -15.03 | -13.08 |
Endosome | -4.14 | -1.10 | -3.56 | -1.16 | -3.20 | -3.29 | 0.03 | -4.37 | -1.13 | -4.12 | -4.73 | -0.80 | -5.44 | -1.62 | -5.06 |
Golgi | -2.83 | 1.60 | -2.43 | 0.21 | -3.17 | -0.69 | 1.14 | -1.30 | -0.90 | -2.85 | -2.54 | 1.82 | -2.65 | -0.61 | -4.12 |
Lipid Particles | 0.28 | 6.81 | 6.55 | 6.90 | -1.77 | 1.65 | 4.99 | 4.82 | 6.76 | -1.08 | 1.74 | 8.42 | 8.09 | 9.60 | -2.08 |
Mitochondria | -1.78 | -0.05 | -3.98 | -3.10 | -4.87 | -5.31 | 0.12 | -5.71 | 1.36 | -6.55 | -4.70 | 0.16 | -5.76 | -0.37 | -7.48 |
Mitotic Spindle | -1.23 | -5.95 | -8.91 | -8.39 | -5.85 | -3.47 | -1.91 | -11.94 | -10.27 | -11.14 | -3.08 | -6.01 | -14.68 | -13.21 | -11.62 |
None | -9.36 | 5.55 | 6.55 | 12.54 | 2.04 | 0.08 | 2.36 | 1.56 | 1.59 | -1.04 | -7.45 | 5.73 | 5.21 | 11.54 | -0.69 |
Nuclear Periphery | 0.58 | -3.93 | -6.95 | -7.51 | -5.60 | -2.55 | -0.04 | -11.06 | -5.80 | -11.03 | -1.39 | -3.37 | -11.32 | -9.18 | -10.34 |
Nuclear Periphery Foci | -2.49 | 1.69 | -1.84 | 0.39 | -3.64 | -1.67 | 1.65 | -1.92 | 0.34 | -3.95 | -2.44 | 2.20 | -2.13 | 0.43 | -5.19 |
Nucleolus | -6.66 | 1.58 | 2.80 | 8.79 | 1.44 | -1.58 | 4.43 | 1.67 | 3.79 | -3.47 | -6.00 | 3.97 | 2.75 | 8.89 | -1.20 |
Nucleus | 10.55 | 0.17 | 3.47 | -6.74 | 3.54 | 3.43 | -7.66 | 9.44 | 6.56 | 18.69 | 8.49 | -4.29 | 8.66 | 0.17 | 13.43 |
Peroxisomes | -4.22 | 3.82 | 3.92 | 6.21 | 0.24 | -2.94 | 3.67 | 2.53 | 5.39 | -2.29 | -5.03 | 5.17 | 4.39 | 8.25 | -1.67 |
Vacuole | -5.47 | -9.67 | -10.07 | -7.81 | -5.81 | -5.05 | -3.47 | -11.43 | -8.09 | -9.21 | -6.95 | -9.92 | -15.18 | -11.17 | -10.19 |
Vacuole Periphery | -5.67 | -2.87 | -4.17 | -3.29 | -3.79 | -4.02 | 0.56 | -3.43 | -1.24 | -3.48 | -6.00 | -2.64 | -5.29 | -3.45 | -4.85 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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