Standard name | |
---|---|
Human Ortholog | |
Description | FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 1 | 1 | 2 | 4 | 3 | 6 | 11 |
Bud | 0 | 2 | 1 | 0 | 0 | 2 | 2 | 3 | – | 7 | 4 | 7 | 0 | 0 | 0 | 0 | 4 | 0 | 6 | 2 | 4 | 2 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | 13 | 14 | 9 | 1 | 0 | 1 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 2 | 9 | 14 | 16 | 10 | 7 | 8 | – | 5 | 9 | 3 | 7 | 15 | 13 | 45 | 43 | 23 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 1 | 3 | 1 | 2 | 2 | 2 | 5 | – | 2 | 5 | 0 | 0 | 0 | 1 | 19 | 17 | 8 | 2 | 1 | 1 | 1 | 2 | 2 |
Endosome | 5 | 0 | 35 | 22 | 57 | 26 | 32 | 56 | – | 6 | 10 | 16 | 9 | 15 | 15 | 30 | 34 | 29 | 12 | 10 | 13 | 11 | 16 | 29 |
Golgi | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 4 | 3 | 3 | 3 | 7 | 11 | 19 |
Mitochondria | 34 | 54 | 107 | 112 | 133 | 372 | 408 | 428 | – | 546 | 488 | 545 | 4 | 6 | 1 | 46 | 46 | 28 | 13 | 11 | 22 | 33 | 59 | 95 |
Nucleus | 10 | 10 | 4 | 18 | 8 | 12 | 23 | 33 | – | 25 | 23 | 23 | 3 | 3 | 0 | 27 | 54 | 14 | 2 | 4 | 1 | 2 | 0 | 3 |
Nuclear Periphery | 504 | 376 | 477 | 261 | 248 | 380 | 414 | 472 | – | 414 | 377 | 559 | 129 | 171 | 108 | 342 | 247 | 227 | 600 | 466 | 550 | 320 | 275 | 289 |
Nucleolus | 54 | 36 | 94 | 113 | 127 | 236 | 290 | 358 | – | 274 | 252 | 343 | 25 | 35 | 32 | 68 | 56 | 59 | 5 | 7 | 1 | 3 | 2 | 3 |
Peroxisomes | 1 | 0 | 2 | 0 | 2 | 1 | 5 | 3 | – | 2 | 0 | 5 | 0 | 5 | 10 | 1 | 0 | 2 | 0 | 0 | 1 | 5 | 8 | 11 |
SpindlePole | 0 | 0 | 4 | 1 | 2 | 9 | 10 | 12 | – | 5 | 6 | 6 | 1 | 8 | 16 | 4 | 10 | 2 | 2 | 2 | 3 | 2 | 1 | 3 |
Vac/Vac Membrane | 263 | 169 | 107 | 40 | 49 | 38 | 51 | 69 | – | 51 | 43 | 38 | 45 | 55 | 38 | 247 | 179 | 136 | 18 | 39 | 10 | 10 | 12 | 18 |
Unique Cell Count | 624 | 499 | 586 | 396 | 449 | 713 | 856 | 1031 | 928 | 770 | 984 | 168 | 246 | 174 | 635 | 502 | 373 | 673 | 553 | 623 | 406 | 402 | 493 | |
Labelled Cell Count | 880 | 650 | 845 | 582 | 644 | 1089 | 1246 | 1450 | 1350 | 1232 | 1555 | 224 | 317 | 237 | 835 | 692 | 533 | 673 | 553 | 623 | 406 | 402 | 493 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.1 | 13.2 | 11.0 | 9.3 | 9.5 | 7.9 | 7.8 | 8.7 | – | 7.7 | 7.5 | 7.7 | 12.8 | 12.2 | 13.1 | 9.6 | 9.6 | 11.9 | 13.1 | 14.0 | 14.8 |
Std Deviation (1e-4) | 2.8 | 3.0 | 2.7 | 2.7 | 2.5 | 2.2 | 2.2 | 2.6 | – | 2.3 | 2.5 | 2.2 | 3.2 | 3.3 | 4.7 | 2.4 | 3.1 | 3.4 | 3.0 | 3.1 | 3.1 |
Intensity Change (Log2) | – | – | – | -0.24 | -0.21 | -0.46 | -0.49 | -0.33 | – | -0.51 | -0.54 | -0.5 | 0.23 | 0.16 | 0.26 | -0.19 | -0.19 | 0.12 | 0.26 | 0.35 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 2.9 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.0 | 2.1 | -0.2 | -1.3 | -1.4 | – | -2.0 | -0.6 | 0 | 0 | 3.6 | 4.1 | 4.7 | 5.4 | 3.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.5 | 0 |
Endosome | -0.3 | 3.8 | -2.0 | -2.0 | -0.5 | – | -6.2 | -4.8 | -4.7 | -0.3 | 0.1 | 1.2 | -1.0 | 0.5 | 1.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.7 | 4.3 | 12.6 | 11.4 | 9.6 | – | 15.5 | 16.6 | 14.4 | -5.1 | -6.1 | -5.9 | -5.8 | -4.3 | -4.7 |
Nucleus | 4.0 | 1.6 | 1.6 | 2.8 | 3.3 | – | 2.8 | 3.0 | 2.4 | 0 | 0 | 0 | 4.0 | 7.4 | 3.4 |
Nuclear Periphery | -5.5 | -9.1 | -10.6 | -12.7 | -14.0 | – | -14.2 | -12.3 | -9.9 | -1.3 | -3.8 | -5.3 | -10.2 | -11.2 | -7.0 |
Nucleolus | 4.7 | 4.8 | 7.0 | 7.5 | 8.0 | – | 6.0 | 7.0 | 8.0 | -0.4 | -0.7 | 0.7 | -2.7 | -2.3 | -0.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1.0 | 0.9 | 0.9 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 |
Vacuole | -3.5 | -3.3 | -7.4 | -7.3 | -7.2 | – | -7.9 | -7.4 | -9.5 | 2.4 | 1.4 | 1.1 | 7.9 | 6.5 | 6.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.1221 | 8.4975 | 9.8258 | 8.2101 | 8.6629 | 9.3397 | 10.6812 | 12.2598 | 11.3096 | 10.3405 | 10.2216 | 11.4702 | 11.842 | 12.608 | 11.6746 | 11.784 | 10.7505 | 11.8339 |
Actin | 0.0528 | 0.0012 | 0.0007 | 0.0314 | 0.0041 | 0.0059 | 0.0201 | 0.001 | 0.012 | 0.0099 | 0.0037 | 0.0035 | 0.0036 | 0.0003 | 0.0058 | 0.0082 | 0.0076 | 0.0001 |
Bud | 0.0005 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0007 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 |
Bud Neck | 0.0008 | 0.0001 | 0 | 0.0005 | 0.0014 | 0.0001 | 0.006 | 0.0001 | 0.0008 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.001 | 0.0002 | 0.0007 | 0 |
Bud Periphery | 0.001 | 0.0003 | 0.0001 | 0.0012 | 0.0002 | 0.0001 | 0.0004 | 0.0005 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0007 | 0.0008 | 0 |
Bud Site | 0.0008 | 0.001 | 0 | 0.0006 | 0.0003 | 0.0001 | 0.0038 | 0.0006 | 0.003 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0 | 0.0004 | 0.0001 | 0.0011 | 0 |
Cell Periphery | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 |
Cytoplasm | 0.0164 | 0.0076 | 0.0003 | 0.001 | 0.0113 | 0.0003 | 0.0097 | 0.012 | 0.0008 | 0.0002 | 0.0016 | 0.0006 | 0.0131 | 0.0001 | 0.0007 | 0.0001 | 0.0035 | 0 |
Cytoplasmic Foci | 0.002 | 0.0006 | 0 | 0.0053 | 0.0069 | 0.0004 | 0.0132 | 0.0001 | 0.0064 | 0.0086 | 0.004 | 0.0006 | 0.0093 | 0 | 0.0011 | 0.0002 | 0.0064 | 0 |
Eisosomes | 0.0005 | 0 | 0 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0054 | 0.0023 | 0.0017 | 0.0078 | 0.0018 | 0.0003 | 0.0018 | 0.0023 | 0.0023 | 0.0042 | 0.0028 | 0.001 | 0.0052 | 0.0017 | 0.002 | 0.0066 | 0.01 | 0.0004 |
Endosome | 0.0132 | 0.0035 | 0.0005 | 0.0097 | 0.0065 | 0.0022 | 0.0121 | 0.0008 | 0.0158 | 0.0073 | 0.0243 | 0.0042 | 0.012 | 0.0007 | 0.0011 | 0.0026 | 0.0196 | 0.0001 |
Golgi | 0.0096 | 0.0007 | 0.0001 | 0.004 | 0.001 | 0.0037 | 0.0044 | 0.0002 | 0.0041 | 0.0029 | 0.0038 | 0.0016 | 0.0016 | 0.0002 | 0.0007 | 0.0023 | 0.0034 | 0.0001 |
Lipid Particles | 0.0119 | 0.0073 | 0.0003 | 0.0498 | 0.0021 | 0.0018 | 0.0227 | 0.0017 | 0.0047 | 0.0209 | 0.0207 | 0.0017 | 0.0084 | 0.0017 | 0.0031 | 0.0071 | 0.0334 | 0.0008 |
Mitochondria | 0.0174 | 0.0024 | 0.002 | 0.0087 | 0.0027 | 0.0019 | 0.0025 | 0.0083 | 0.007 | 0.0084 | 0.0061 | 0.0062 | 0.0016 | 0.0141 | 0.0037 | 0.0088 | 0.0051 | 0.0015 |
None | 0.0017 | 0.0005 | 0.0002 | 0.0016 | 0.0252 | 0.0001 | 0.0062 | 0.0009 | 0.0005 | 0.0005 | 0.002 | 0.0009 | 0.0235 | 0.0002 | 0.0033 | 0.0007 | 0.002 | 0.0001 |
Nuclear Periphery | 0.7608 | 0.7103 | 0.8822 | 0.7608 | 0.7809 | 0.9178 | 0.81 | 0.9053 | 0.8635 | 0.8355 | 0.8654 | 0.9558 | 0.8667 | 0.9023 | 0.9114 | 0.8955 | 0.8281 | 0.9735 |
Nucleolus | 0.0026 | 0.0064 | 0.0004 | 0.0153 | 0.0028 | 0.0021 | 0.0055 | 0.0004 | 0.0035 | 0.001 | 0.0058 | 0.0002 | 0.0034 | 0.0002 | 0.0003 | 0.0008 | 0.0045 | 0.0002 |
Nucleus | 0.0608 | 0.1881 | 0.0665 | 0.0488 | 0.1018 | 0.0446 | 0.0065 | 0.014 | 0.0147 | 0.0068 | 0.0093 | 0.0023 | 0.0113 | 0.0176 | 0.0047 | 0.005 | 0.0176 | 0.0013 |
Peroxisomes | 0.0024 | 0.0002 | 0 | 0.0004 | 0.0003 | 0.0001 | 0.006 | 0 | 0.0021 | 0.028 | 0.0013 | 0.0012 | 0.0055 | 0 | 0.0004 | 0.0002 | 0.0005 | 0 |
Punctate Nuclear | 0.0022 | 0.0026 | 0.0006 | 0.0117 | 0.0171 | 0.0009 | 0.0272 | 0.0005 | 0.0012 | 0.0029 | 0.0129 | 0.0012 | 0.0029 | 0.0004 | 0.0009 | 0.0015 | 0.016 | 0.0002 |
Vacuole | 0.0053 | 0.0246 | 0.0007 | 0.0023 | 0.0026 | 0.0008 | 0.0024 | 0.0015 | 0.0107 | 0.0029 | 0.002 | 0.0002 | 0.0022 | 0.0015 | 0.0009 | 0.0017 | 0.0056 | 0.0001 |
Vacuole Periphery | 0.0318 | 0.0398 | 0.0436 | 0.0384 | 0.0308 | 0.0167 | 0.0388 | 0.049 | 0.0453 | 0.0592 | 0.0331 | 0.0183 | 0.0286 | 0.0585 | 0.0578 | 0.0569 | 0.0334 | 0.0214 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 47.9577 | 39.6081 | 48.0942 | 55.0276 | 46.8384 | 46.0441 | 48.9219 | 50.9874 | 49.1416 | 53.7104 |
Translational Efficiency | 1.3809 | 1.3809 | 1.2383 | 1.1008 | 1.2746 | 1.1891 | 0.9901 | 0.9516 | 1.0254 | 1.0029 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
255 | 1188 | 249 | 1177 | 2326 | 2265 | 2032 | 1769 | 2581 | 3453 | 2281 | 2946 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1233.02 | 1076.59 | 1382.15 | 1274.92 | 970.09 | 1200.08 | 1264.47 | 1295.24 | 996.07 | 1157.59 | 1277.32 | 1287.12 |
Standard Deviation | 172.74 | 251.72 | 209.09 | 268.51 | 165.54 | 202.70 | 244.84 | 224.13 | 183.85 | 228.46 | 243.97 | 243.04 |
Intensity Change Log 2 | — | -0.195727 | 0.164718 | 0.048211 | — | 0.306940 | 0.382342 | 0.417029 | — | 0.047384 | 0.264610 | 0.222317 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000450 | 0.000835 | 0.000693 | 0.000622 | 0.000758 | 0.000353 | 0.000287 | 0.000232 | 0.000728 | 0.000519 | 0.000331 | 0.000387 |
Bud Neck | 0.002593 | 0.000886 | 0.001441 | 0.003331 | 0.000966 | 0.000242 | 0.000674 | 0.000951 | 0.001127 | 0.000464 | 0.000758 | 0.001902 |
Bud Site | 0.000741 | 0.000514 | 0.000169 | 0.000806 | 0.000378 | 0.000093 | 0.000072 | 0.000239 | 0.000414 | 0.000238 | 0.000082 | 0.000465 |
Cell Periphery | 0.000097 | 0.000211 | 0.000047 | 0.000145 | 0.000213 | 0.000035 | 0.000035 | 0.000050 | 0.000202 | 0.000095 | 0.000037 | 0.000088 |
Cytoplasm | 0.000500 | 0.003246 | 0.000355 | 0.001939 | 0.001607 | 0.000302 | 0.000431 | 0.000448 | 0.001498 | 0.001315 | 0.000423 | 0.001044 |
Cytoplasmic Foci | 0.003794 | 0.002224 | 0.000301 | 0.004049 | 0.001573 | 0.000683 | 0.000333 | 0.001438 | 0.001792 | 0.001213 | 0.000330 | 0.002481 |
Eisosomes | 0.000022 | 0.000012 | 0.000009 | 0.000012 | 0.000023 | 0.000005 | 0.000003 | 0.000003 | 0.000023 | 0.000007 | 0.000004 | 0.000007 |
Endoplasmic Reticulum | 0.005460 | 0.008617 | 0.007086 | 0.004440 | 0.008816 | 0.002835 | 0.002450 | 0.003450 | 0.008485 | 0.004824 | 0.002956 | 0.003845 |
Endosome | 0.003046 | 0.004499 | 0.000999 | 0.005791 | 0.004091 | 0.001134 | 0.000734 | 0.002323 | 0.003988 | 0.002292 | 0.000763 | 0.003709 |
Golgi | 0.001549 | 0.001302 | 0.000169 | 0.004542 | 0.000764 | 0.000473 | 0.000254 | 0.001140 | 0.000842 | 0.000758 | 0.000244 | 0.002499 |
Lipid Particles | 0.006701 | 0.009021 | 0.000868 | 0.010376 | 0.020048 | 0.005082 | 0.001058 | 0.008054 | 0.018729 | 0.006437 | 0.001037 | 0.008982 |
Mitochondria | 0.004526 | 0.006797 | 0.002153 | 0.004768 | 0.016348 | 0.004781 | 0.001985 | 0.003440 | 0.015180 | 0.005475 | 0.002003 | 0.003971 |
Mitotic Spindle | 0.018486 | 0.003176 | 0.005037 | 0.027218 | 0.001795 | 0.001285 | 0.001818 | 0.008285 | 0.003444 | 0.001935 | 0.002169 | 0.015849 |
None | 0.000210 | 0.005897 | 0.000450 | 0.001027 | 0.004138 | 0.000492 | 0.000547 | 0.000625 | 0.003750 | 0.002351 | 0.000537 | 0.000786 |
Nuclear Periphery | 0.657415* | 0.541425* | 0.618649* | 0.529833* | 0.570793* | 0.718733* | 0.698902* | 0.660938* | 0.579351* | 0.657731* | 0.690142* | 0.608558* |
Nuclear Periphery Foci | 0.264132* | 0.341103* | 0.281045* | 0.347436* | 0.313551* | 0.237218* | 0.257797* | 0.280991* | 0.308668* | 0.272959* | 0.260335* | 0.307538* |
Nucleolus | 0.007765 | 0.020291 | 0.023386 | 0.008392 | 0.011718 | 0.003534 | 0.008854 | 0.002537 | 0.011328 | 0.009300 | 0.010440 | 0.004876 |
Nucleus | 0.004442 | 0.023626 | 0.053042 | 0.011849 | 0.010613 | 0.004255 | 0.014481 | 0.002615 | 0.010004 | 0.010920 | 0.018690 | 0.006304 |
Peroxisomes | 0.000655 | 0.000565 | 0.000587 | 0.000471 | 0.000660 | 0.000456 | 0.000125 | 0.000072 | 0.000659 | 0.000493 | 0.000175 | 0.000231 |
Vacuole | 0.007559 | 0.010014 | 0.001688 | 0.013922 | 0.011148 | 0.004855 | 0.002339 | 0.006322 | 0.010794 | 0.006630 | 0.002268 | 0.009359 |
Vacuole Periphery | 0.009855 | 0.015739 | 0.001824 | 0.019032 | 0.019998 | 0.013153 | 0.006822 | 0.015845 | 0.018995 | 0.014043 | 0.006276 | 0.017118 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.47 | -1.41 | -0.73 | 0.90 | 0.65 | 2.94 | 7.23 | 8.07 | 0.90 | 1.18 | 1.55 | 6.28 | 4.15 | 1.07 | -0.74 |
Bud Neck | 2.19 | 1.43 | -0.77 | -5.37 | -3.70 | 6.67 | 2.09 | 0.04 | -4.71 | -1.62 | 5.12 | 2.48 | -3.40 | -7.03 | -5.30 |
Bud Site | 0.49 | 1.91 | 0.01 | -0.70 | -3.81 | 4.37 | 5.28 | 1.72 | -2.11 | -2.70 | 1.41 | 5.46 | -0.53 | -1.75 | -5.12 |
Cell Periphery | -1.65 | 0.95 | -0.78 | 1.41 | -3.89 | 9.19 | 9.09 | 8.05 | -1.90 | -1.73 | 4.66 | 8.96 | 5.49 | 0.39 | -4.69 |
Cytoplasm | -3.07 | 0.64 | -2.77 | 1.35 | -3.44 | 3.17 | 2.95 | 2.82 | -0.66 | -0.11 | 0.42 | 2.99 | 1.18 | 0.79 | -2.83 |
Cytoplasmic Foci | 1.06 | 2.56 | -0.15 | -2.31 | -6.04 | 3.42 | 4.88 | 0.30 | -1.98 | -2.89 | 1.95 | 5.52 | -1.66 | -3.34 | -6.11 |
Eisosomes | 0.78 | 0.88 | 0.75 | -0.07 | -0.46 | 10.40 | 11.88 | 11.52 | 1.49 | -0.35 | 7.45 | 9.31 | 6.85 | 0.23 | -2.23 |
Endoplasmic Reticulum | -1.91 | -1.04 | 0.81 | 4.51 | 2.51 | 9.46 | 9.85 | 7.58 | -1.39 | -2.11 | 5.85 | 9.05 | 7.47 | 2.14 | -2.13 |
Endosome | -1.28 | 2.40 | -2.34 | -1.39 | -6.73 | 6.35 | 7.70 | 2.95 | -2.69 | -3.85 | 3.65 | 8.04 | 0.47 | -3.22 | -7.79 |
Golgi | 0.31 | 2.27 | -2.77 | -3.42 | -4.91 | 1.02 | 2.86 | -1.32 | -2.11 | -4.04 | 0.30 | 3.47 | -3.95 | -4.11 | -5.93 |
Lipid Particles | -1.01 | 3.27 | -1.49 | -0.63 | -5.92 | 9.47 | 12.99 | 6.42 | -2.23 | -5.84 | 8.42 | 13.29 | 5.99 | -2.23 | -8.16 |
Mitochondria | -0.73 | 0.79 | -0.09 | 1.47 | -2.33 | 8.46 | 10.79 | 9.36 | 1.63 | -1.95 | 7.70 | 10.66 | 8.77 | 2.05 | -2.96 |
Mitotic Spindle | 2.74 | 2.38 | -1.35 | -7.37 | -6.49 | 0.86 | -0.01 | -4.03 | -4.34 | -4.03 | 1.99 | 1.70 | -7.19 | -8.55 | -8.41 |
None | -4.48 | -1.52 | -3.77 | 3.77 | -2.26 | 6.89 | 6.64 | 5.71 | -0.39 | -0.21 | 2.22 | 6.58 | 5.72 | 3.17 | -1.03 |
Nuclear Periphery | 6.37 | 1.65 | 7.11 | 1.17 | 4.30 | -22.55* | -17.55 | -12.12 | 8.63 | 5.11 | -12.22 | -15.67 | -4.12 | 7.92 | 11.77 |
Nuclear Periphery Foci | -5.75 | -0.93 | -6.24 | -0.65 | -4.46 | 13.50* | 8.96 | 5.22 | -7.67 | -3.69 | 6.67 | 8.23 | 0.22 | -6.71 | -8.30 |
Nucleolus | -3.09 | -2.86 | -0.21 | 4.59 | 3.25 | 8.30 | 2.11 | 8.97 | 1.37 | 5.49 | 1.76 | 0.58 | 6.01 | 4.19 | 4.59 |
Nucleus | -5.92 | -4.79 | -2.88 | 3.43 | 4.09 | 4.94 | -1.95 | 6.07 | 1.21 | 6.16 | -0.56 | -4.15 | 2.63 | 3.06 | 5.81 |
Peroxisomes | 0.32 | 0.17 | 0.59 | 0.46 | 0.63 | 0.49 | 8.15 | 8.86 | 1.38 | 2.11 | 0.61 | 7.23 | 4.18 | 1.33 | -0.64 |
Vacuole | -0.81 | 2.84 | -2.45 | -2.43 | -9.09 | 6.29 | 10.75 | 4.66 | -1.73 | -5.90 | 4.48 | 11.13 | 1.24 | -3.36 | -10.45 |
Vacuole Periphery | -1.85 | 2.78 | -2.81 | -1.65 | -11.18 | 4.73 | 11.50 | 2.74 | -1.77 | -7.21 | 3.95 | 11.88 | 1.41 | -2.53 | -10.49 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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