Standard name | |
---|---|
Human Ortholog | |
Description | Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SEC24 has a paralog, SFB2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 115 | 45 | 282 | 205 | 278 | 285 | 365 | 199 | – | 373 | 179 | 368 | 108 | 186 | 114 | 125 | 213 | 108 | 116 | 39 | 372 | 163 | 355 | 369 |
Bud | 7 | 16 | 14 | 6 | 22 | 19 | 31 | 31 | – | 48 | 21 | 54 | 9 | 10 | 7 | 14 | 18 | 16 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 6 | 54 | 59 | 43 | 47 | 50 | 24 | – | 45 | 12 | 46 | 30 | 52 | 40 | 12 | 9 | 15 | 1 | 1 | 11 | 6 | 30 | 30 |
Cytoplasm | 8 | 24 | 22 | 24 | 20 | 23 | 23 | 15 | – | 13 | 10 | 11 | 33 | 34 | 35 | 28 | 70 | 59 | 1 | 3 | 1 | 2 | 4 | 5 |
Endoplasmic Reticulum | 211 | 78 | 375 | 274 | 379 | 414 | 640 | 489 | – | 502 | 173 | 307 | 277 | 394 | 261 | 3 | 22 | 11 | 115 | 72 | 92 | 73 | 175 | 211 |
Endosome | 0 | 0 | 1 | 0 | 2 | 0 | 3 | 1 | – | 4 | 0 | 4 | 0 | 0 | 1 | 1 | 6 | 3 | 0 | 1 | 1 | 0 | 1 | 2 |
Golgi | 167 | 13 | 340 | 252 | 432 | 304 | 617 | 546 | – | 598 | 205 | 404 | 92 | 150 | 126 | 125 | 280 | 183 | 37 | 21 | 68 | 49 | 88 | 144 |
Mitochondria | 11 | 6 | 30 | 48 | 97 | 226 | 232 | 241 | – | 468 | 243 | 451 | 0 | 0 | 0 | 20 | 63 | 32 | 0 | 1 | 3 | 4 | 10 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 1 | 2 | 5 | 18 | – | 18 | 7 | 10 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 7 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 5 | – | 3 | 2 | 4 | 1 | 3 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 0 |
Unique Cell Count | 276 | 127 | 548 | 432 | 624 | 634 | 1011 | 952 | 1069 | 422 | 874 | 362 | 529 | 378 | 205 | 439 | 282 | 276 | 143 | 557 | 304 | 677 | 781 | |
Labelled Cell Count | 528 | 188 | 1119 | 869 | 1274 | 1321 | 1972 | 1577 | 2075 | 853 | 1661 | 551 | 829 | 585 | 328 | 689 | 429 | 276 | 143 | 557 | 304 | 677 | 781 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.3 | 20.2 | 21.7 | 22.2 | 21.3 | 19.1 | 19.2 | 19.2 | – | 18.6 | 18.3 | 18.2 | 25.2 | 25.1 | 26.0 | 16.4 | 17.9 | 21.8 | 24.8 | 25.1 | 27.5 |
Std Deviation (1e-4) | 4.0 | 3.4 | 3.5 | 3.7 | 3.6 | 3.3 | 3.5 | 4.0 | – | 4.0 | 4.3 | 4.2 | 4.8 | 4.5 | 5.7 | 5.4 | 6.2 | 8.5 | 3.7 | 4.4 | 5.3 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.03 | -0.18 | -0.18 | -0.18 | – | -0.22 | -0.24 | -0.25 | 0.22 | 0.21 | 0.26 | -0.4 | -0.27 | 0.01 | 0.19 | 0.21 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.2 | -2.4 | -2.2 | -5.9 | -12.2 | – | -6.4 | -2.8 | -3.4 | -6.5 | -5.4 | -6.4 | 2.3 | -0.9 | -3.6 |
Bud | -1.3 | 1.0 | 0.5 | 0.6 | 0.8 | – | 1.9 | 2.0 | 3.1 | -0.1 | -0.7 | -0.7 | 2.8 | 1.4 | 2.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.9 | -1.8 | -1.5 | -3.7 | -6.2 | – | -4.5 | -4.3 | -3.3 | -0.8 | 0 | 0.4 | -1.7 | -5.0 | -2.2 |
Cytoplasm | 1.1 | -0.7 | -0.3 | -2.0 | -2.9 | – | -3.7 | -1.4 | -3.4 | 3.2 | 1.8 | 3.3 | 4.7 | 6.4 | 7.8 |
Endoplasmic Reticulum | -1.6 | -2.7 | -1.1 | -2.0 | -6.4 | – | -8.2 | -8.5 | -12.2 | 2.6 | 2.2 | 0.2 | -16.4 | -20.2 | -17.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.2 | 2.6 | -4.9 | -0.4 | -1.8 | – | -2.4 | -4.2 | -5.8 | -10.8 | -11.1 | -8.6 | -0.3 | 0.6 | 0.8 |
Mitochondria | 3.2 | 5.5 | 12.6 | 8.8 | 9.6 | – | 15.8 | 17.9 | 17.9 | -4.5 | -5.5 | -4.6 | 2.1 | 4.7 | 3.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 3.2 | – | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.7559 | 27.3253 | 25.7326 | 26.2441 | 24.8533 | 25.2174 | 18.7696 | 27.0943 | 24.5067 | 24.5484 | 23.2121 | 24.5331 | 22.1749 | 25.9854 | 23.288 | 24.8025 | 23.1423 | 23.6643 |
Actin | 0.0704 | 0.0423 | 0.0393 | 0.0096 | 0.0444 | 0.0292 | 0.0827 | 0.0964 | 0.1331 | 0.1146 | 0.0669 | 0.043 | 0.1254 | 0.0764 | 0.1332 | 0.0351 | 0.049 | 0.0539 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0006 | 0.0004 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.001 | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.001 | 0.0012 | 0.0002 | 0.0008 | 0.0012 | 0.0005 | 0.0005 | 0.0007 | 0.0004 | 0.0017 | 0.0004 | 0.0002 | 0.0011 |
Bud Periphery | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0005 | 0.0003 | 0.0001 | 0.0006 | 0.0004 | 0.0009 | 0.0002 | 0 | 0.0002 |
Bud Site | 0.0004 | 0.0022 | 0.0007 | 0 | 0.0003 | 0 | 0.0059 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0023 | 0.0016 | 0.0067 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0007 | 0.0012 | 0.0005 | 0.0006 | 0.0002 | 0.0005 | 0.0004 | 0.0002 | 0.0005 | 0.0009 | 0.0004 | 0.0003 | 0.0005 | 0.0019 | 0.0018 | 0.0008 | 0.0002 | 0.0003 |
Cytoplasm | 0.003 | 0.0149 | 0.0013 | 0.0003 | 0.0016 | 0.0038 | 0.0017 | 0.0008 | 0.001 | 0.0007 | 0.0007 | 0.0006 | 0.0033 | 0.0025 | 0.0017 | 0.0006 | 0.0005 | 0.001 |
Cytoplasmic Foci | 0.01 | 0.0107 | 0.0073 | 0.0028 | 0.0076 | 0.005 | 0.0242 | 0.009 | 0.0038 | 0.0033 | 0.0025 | 0.0029 | 0.0063 | 0.0107 | 0.0113 | 0.0043 | 0.0056 | 0.0039 |
Eisosomes | 0.0034 | 0.0049 | 0.0016 | 0.0051 | 0.0018 | 0.0048 | 0.0026 | 0.0007 | 0.003 | 0.0052 | 0.0026 | 0.0008 | 0.0022 | 0.0013 | 0.0089 | 0.0045 | 0.0018 | 0.0006 |
Endoplasmic Reticulum | 0.0097 | 0.016 | 0.0103 | 0.0084 | 0.0068 | 0.0196 | 0.0031 | 0.0018 | 0.0087 | 0.0113 | 0.0092 | 0.0084 | 0.0174 | 0.0189 | 0.0091 | 0.01 | 0.0048 | 0.0148 |
Endosome | 0.0299 | 0.0208 | 0.0172 | 0.0122 | 0.0226 | 0.0199 | 0.035 | 0.0126 | 0.0135 | 0.0188 | 0.0221 | 0.012 | 0.024 | 0.0225 | 0.0277 | 0.0283 | 0.0173 | 0.0279 |
Golgi | 0.8408 | 0.8802 | 0.9136 | 0.9563 | 0.8937 | 0.9121 | 0.7652 | 0.876 | 0.8135 | 0.8341 | 0.8879 | 0.915 | 0.7836 | 0.8338 | 0.7707 | 0.8648 | 0.9166 | 0.8925 |
Lipid Particles | 0.0115 | 0.0046 | 0.0041 | 0.0031 | 0.0035 | 0.0023 | 0.0341 | 0.0014 | 0.0026 | 0.0041 | 0.001 | 0.0041 | 0.0119 | 0.0118 | 0.0099 | 0.0449 | 0.0026 | 0.0008 |
Mitochondria | 0.0044 | 0.0003 | 0.0013 | 0.0004 | 0.0057 | 0.0007 | 0.0038 | 0.0003 | 0.0035 | 0.0029 | 0.0045 | 0.0018 | 0.0061 | 0.014 | 0.0061 | 0.0039 | 0.0006 | 0.0015 |
None | 0.0015 | 0.0001 | 0.0003 | 0 | 0.0007 | 0 | 0.0021 | 0 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0004 | 0.0011 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0054 | 0.0003 | 0.0003 | 0.0004 | 0.0049 | 0.0004 | 0.0112 | 0.0001 | 0.0094 | 0.0006 | 0.0002 | 0.0074 | 0.01 | 0.0008 | 0.0033 | 0.0005 | 0.0001 | 0.0004 |
Nucleolus | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0 |
Nucleus | 0.0005 | 0.0001 | 0 | 0 | 0.0009 | 0 | 0.0042 | 0 | 0.0019 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0 | 0 | 0 |
Peroxisomes | 0.0011 | 0.0001 | 0.0009 | 0 | 0.0009 | 0 | 0.0076 | 0.0001 | 0.0016 | 0 | 0.0001 | 0.0001 | 0.0013 | 0.001 | 0.001 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0033 | 0.0001 | 0.0001 | 0 | 0.0024 | 0 | 0.0106 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0019 | 0 | 0 | 0 |
Vacuole | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.001 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0007 | 0.0003 | 0.0007 | 0.0004 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0017 | 0.0002 | 0.0004 | 0.0004 | 0.0006 | 0.0003 | 0.0014 | 0.0001 | 0.0006 | 0.0008 | 0.0006 | 0.0026 | 0.0017 | 0.0004 | 0.0012 | 0.001 | 0.0003 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 106.3331 | 122.0113 | 101.8548 | 115.5104 | 98.2549 | 91.6021 | 113.7724 | 111.2196 | 100.95 | 114.0765 |
Translational Efficiency | 1.4114 | 1.2844 | 1.4044 | 1.0812 | 1.3486 | 1.6687 | 1.3094 | 1.2698 | 1.3236 | 1.1835 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
172 | 23 | 2713 | 249 | 264 | 1205 | 478 | 1895 | 436 | 1228 | 3191 | 2144 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2172.14 | 293.20 | 2155.42 | 2438.95 | 1310.39 | 1634.78 | 2191.41 | 2260.44 | 1650.35 | 1609.65 | 2160.81 | 2281.17 |
Standard Deviation | 347.51 | 16.26 | 339.75 | 551.15 | 233.70 | 280.99 | 320.60 | 394.54 | 508.03 | 332.51 | 337.20 | 419.69 |
Intensity Change Log 2 | — | -2.889160 | -0.011148 | 0.167143 | — | 0.319100 | 0.741863 | 0.786607 | — | -0.853046 | 0.319828 | 0.432338 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.027796 | 0.000034 | 0.008821 | 0.009525 | 0.037955 | 0.038069 | 0.012701 | 0.014193 | 0.033947 | 0.037356 | 0.009402 | 0.013651 |
Bud Neck | 0.004054 | 0.000008 | 0.000438 | 0.002665 | 0.002386 | 0.001398 | 0.002026 | 0.000841 | 0.003044 | 0.001372 | 0.000676 | 0.001053 |
Bud Site | 0.003239 | 0.000014 | 0.000424 | 0.001364 | 0.001213 | 0.001362 | 0.000832 | 0.001572 | 0.002012 | 0.001337 | 0.000485 | 0.001548 |
Cell Periphery | 0.022611 | 0.000054 | 0.007119 | 0.025390 | 0.004161 | 0.004058 | 0.013457 | 0.018046 | 0.011440 | 0.003983 | 0.008069 | 0.018899 |
Cytoplasm | 0.000053 | 0.000028 | 0.000168 | 0.000061 | 0.000137 | 0.000274 | 0.000039 | 0.000648 | 0.000104 | 0.000269 | 0.000148 | 0.000580 |
Cytoplasmic Foci | 0.002378 | 0.000013 | 0.003928 | 0.004123 | 0.004762 | 0.004085 | 0.000762 | 0.009261 | 0.003821 | 0.004008 | 0.003454 | 0.008664 |
Eisosomes | 0.066325 | 0.000061 | 0.039140 | 0.045987 | 0.038926 | 0.078422 | 0.091812 | 0.067255 | 0.049735 | 0.076954 | 0.047030 | 0.064785 |
Endoplasmic Reticulum | 0.534294* | 0.000001 | 0.344992* | 0.331166* | 0.332553* | 0.341669* | 0.456980* | 0.341779* | 0.412139* | 0.335270* | 0.361767* | 0.340547* |
Endosome | 0.027261 | 0.000001 | 0.020049 | 0.037196 | 0.065502 | 0.033854 | 0.017024 | 0.031072 | 0.050416 | 0.033220 | 0.019596 | 0.031784 |
Golgi | 0.180715* | 0.000004 | 0.447576* | 0.218041* | 0.368906* | 0.399769* | 0.304651* | 0.317103* | 0.294666* | 0.392282* | 0.426167* | 0.305598* |
Lipid Particles | 0.115413* | 0.000021 | 0.115578* | 0.305632* | 0.133717* | 0.091771 | 0.089880 | 0.181774* | 0.126496* | 0.090053 | 0.111729* | 0.196159* |
Mitochondria | 0.000243 | 0.000011 | 0.000095 | 0.000051 | 0.000665 | 0.000637 | 0.000103 | 0.000201 | 0.000499 | 0.000626 | 0.000097 | 0.000183 |
Mitotic Spindle | 0.001507 | 0.000335 | 0.000031 | 0.000166 | 0.000033 | 0.000330 | 0.000052 | 0.000132 | 0.000615 | 0.000330 | 0.000034 | 0.000136 |
None | 0.000095 | 0.999261* | 0.000629 | 0.000105 | 0.000225 | 0.000322 | 0.000205 | 0.000360 | 0.000173 | 0.019032 | 0.000566 | 0.000331 |
Nuclear Periphery | 0.001177 | 0.000000 | 0.000440 | 0.002158 | 0.000219 | 0.000194 | 0.001159 | 0.000984 | 0.000597 | 0.000191 | 0.000548 | 0.001120 |
Nuclear Periphery Foci | 0.000774 | 0.000030 | 0.000172 | 0.001212 | 0.000541 | 0.000163 | 0.000320 | 0.000261 | 0.000633 | 0.000160 | 0.000194 | 0.000372 |
Nucleolus | 0.000003 | 0.000031 | 0.000002 | 0.000002 | 0.001577 | 0.000002 | 0.000002 | 0.000003 | 0.000956 | 0.000003 | 0.000002 | 0.000003 |
Nucleus | 0.000075 | 0.000012 | 0.000072 | 0.000055 | 0.002018 | 0.000071 | 0.000062 | 0.000099 | 0.001252 | 0.000070 | 0.000070 | 0.000094 |
Peroxisomes | 0.000320 | 0.000080 | 0.007933 | 0.001047 | 0.001091 | 0.001374 | 0.001602 | 0.011045 | 0.000787 | 0.001350 | 0.006985 | 0.009884 |
Vacuole | 0.009233 | 0.000001 | 0.001061 | 0.011918 | 0.001381 | 0.001198 | 0.004133 | 0.001900 | 0.004479 | 0.001175 | 0.001521 | 0.003063 |
Vacuole Periphery | 0.002433 | 0.000000 | 0.001330 | 0.002134 | 0.002030 | 0.000978 | 0.002197 | 0.001469 | 0.002189 | 0.000960 | 0.001460 | 0.001546 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.86 | 2.63 | 2.45 | -4.89 | -0.34 | -0.03 | 4.99 | 5.00 | 7.86 | -0.60 | -0.72 | 6.11 | 4.89 | 8.08 | -3.06 |
Bud Neck | 6.45 | 5.75 | 1.67 | -4.89 | -4.08 | 3.39 | 0.93 | 5.68 | 4.37 | 4.19 | 5.24 | 7.82 | 6.46 | 2.29 | -3.97 |
Bud Site | 2.97 | 2.59 | 1.70 | -7.10 | -4.86 | -0.54 | 2.14 | -1.33 | -0.70 | -2.65 | 1.32 | 3.46 | 0.80 | -0.74 | -4.37 |
Cell Periphery | 6.01 | 4.12 | -0.48 | -5.77 | -4.15 | 0.15 | -4.84 | -14.24 | -18.62 | -2.36 | 4.64 | 2.07 | -4.17 | -17.67 | -12.22 |
Cytoplasm | 1.29 | -4.96 | -0.36 | -2.70 | 6.24 | -2.04 | 5.15 | -2.50 | -1.79 | -2.95 | -2.51 | -2.82 | -2.62 | -1.67 | -2.39 |
Cytoplasmic Foci | 2.08 | -1.32 | -1.04 | -3.39 | -0.16 | 0.81 | 6.13 | -4.67 | -5.41 | -10.48 | -0.23 | 0.60 | -5.39 | -5.21 | -7.22 |
Eisosomes | 6.62 | 2.68 | 1.54 | -5.31 | -0.77 | -5.42 | -5.52 | -4.32 | 2.06 | 2.98 | -3.95 | 0.48 | -2.48 | 2.31 | -5.09 |
Endoplasmic Reticulum | 26.19* | 9.01 | 7.72 | -19.91 | 0.81 | -0.52 | -6.26 | -0.54 | 0.00 | 8.69 | 4.96 | 3.55 | 4.96 | -0.54 | 2.99 |
Endosome | 12.52 | 3.27 | -2.88 | -13.86 | -6.34 | 5.33 | 8.33 | 5.93 | 1.81 | -14.23 | 4.36 | 8.29 | 4.95 | 0.94 | -16.76 |
Golgi | 13.73 | -18.86 | -2.03 | -17.00 | 16.59 | -1.82 | 3.46 | 3.22 | 8.82 | -1.02 | -7.10 | -10.62 | -0.87 | 9.44 | 17.29 |
Lipid Particles | 8.61 | -0.01 | -8.71 | -17.72 | -10.87 | 3.16 | 3.16 | -3.59 | -14.50 | -12.39 | 3.59 | 1.53 | -6.73 | -17.18 | -16.00 |
Mitochondria | 1.89 | 1.20 | 1.56 | -3.18 | 3.19 | 0.08 | 2.24 | 1.82 | 1.80 | -2.00 | -0.45 | 2.53 | 1.92 | 1.86 | -2.47 |
Mitotic Spindle | 0.88 | 1.10 | 1.00 | 3.13 | -4.53 | -1.08 | -2.22 | -3.07 | 0.69 | -2.54 | 0.48 | 1.10 | 0.89 | 0.69 | -3.56 |
None | -10122.65* | -16.08 | -0.80 | 10119.52* | 15.71 | -1.46 | 0.57 | -3.35 | -0.59 | -4.44 | -4.88 | -12.02 | -5.03 | 4.84 | 6.21 |
Nuclear Periphery | 9.87 | 6.13 | -2.47 | -5.70 | -4.53 | 1.08 | -12.60 | -14.31 | -15.89 | 2.02 | 7.50 | 0.87 | -6.37 | -14.82 | -8.86 |
Nuclear Periphery Foci | 5.94 | 4.72 | -1.75 | -5.42 | -4.74 | 2.37 | 1.37 | 1.72 | -2.97 | 1.28 | 4.26 | 4.00 | 2.28 | -5.09 | -4.74 |
Nucleolus | -3.04 | 1.39 | 1.06 | 3.19 | -0.80 | 1.00 | 1.00 | 1.00 | -1.03 | -1.29 | 1.00 | 1.00 | 1.00 | -0.19 | -3.40 |
Nucleus | 8.56 | 0.53 | 2.68 | -7.62 | 3.75 | 1.01 | 1.01 | 0.99 | -5.64 | -7.16 | 1.01 | 1.01 | 0.99 | -5.17 | -6.31 |
Peroxisomes | 2.60 | -9.72 | -0.96 | -1.28 | 6.36 | -0.41 | -0.59 | -7.81 | -8.44 | -7.47 | -1.21 | -8.07 | -8.59 | -8.32 | -2.43 |
Vacuole | 4.48 | 3.96 | -1.00 | -6.93 | -6.31 | 0.28 | -3.06 | -1.22 | -1.52 | 2.71 | 3.36 | 3.35 | 1.51 | -3.71 | -5.36 |
Vacuole Periphery | 3.55 | 1.60 | 0.40 | -7.22 | -2.67 | 2.26 | -0.28 | 1.29 | -2.48 | 1.68 | 2.95 | 1.91 | 1.68 | -2.99 | -0.86 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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