Standard name | |
---|---|
Human Ortholog | |
Description | Methyltransferase required for m3U2634 methylation of the 25S rRNA; S-adenosylmethionine-dependent; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 4 | 6 | 5 | 8 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 6 | 0 | 4 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud Site | 0 | 1 | 0 | 1 | 0 | 9 | 6 | 6 | 16 | 13 | 11 | 13 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 4 | 7 | 7 | 30 | 16 | 22 | 21 | 24 | 12 | 18 | 9 | 15 | 18 | 43 | 9 | 18 | 12 | 0 | 0 | 0 | 1 | 1 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 3 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 0 | 0 | 0 | 4 | 12 | 6 | 5 | 4 | 1 | 3 | 0 | 7 | 0 | 5 | 18 | 3 | 3 | 2 | 3 | 0 | 2 | 2 | 15 | 12 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 0 | 1 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 67 | 2 | 6 | 21 | 27 | 129 | 249 | 156 | 274 | 212 | 224 | 181 | 13 | 3 | 3 | 3 | 8 | 8 | 1 | 0 | 2 | 1 | 8 | 15 |
Nucleus | 279 | 117 | 188 | 157 | 300 | 219 | 276 | 256 | 274 | 271 | 178 | 277 | 74 | 94 | 74 | 112 | 112 | 81 | 263 | 107 | 153 | 42 | 48 | 58 |
Nuclear Periphery | 2 | 0 | 1 | 1 | 1 | 0 | 4 | 8 | 16 | 2 | 3 | 6 | 0 | 2 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 3 | 9 |
Nucleolus | 3 | 8 | 2 | 69 | 35 | 23 | 64 | 56 | 46 | 30 | 25 | 29 | 35 | 86 | 79 | 65 | 73 | 63 | 9 | 4 | 13 | 11 | 28 | 25 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 23 | 26 | 19 | 13 | 31 | 11 | 13 | 3 | 9 | 10 | 24 | 36 | 5 | 5 | 2 | 0 | 0 | 0 | 3 | 6 | 9 |
Vac/Vac Membrane | 3 | 6 | 0 | 2 | 11 | 4 | 5 | 7 | 9 | 12 | 5 | 14 | 1 | 1 | 1 | 7 | 9 | 5 | 6 | 10 | 12 | 7 | 29 | 33 |
Unique Cell Count | 293 | 128 | 196 | 241 | 387 | 322 | 451 | 419 | 437 | 405 | 313 | 402 | 116 | 186 | 201 | 187 | 210 | 153 | 295 | 127 | 192 | 73 | 152 | 185 |
Labelled Cell Count | 359 | 138 | 205 | 286 | 444 | 428 | 649 | 555 | 677 | 578 | 475 | 549 | 151 | 237 | 258 | 210 | 236 | 177 | 295 | 127 | 192 | 73 | 152 | 185 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 6.7 | 6.0 | 5.8 | 5.9 | 4.6 | 4.3 | 4.7 | 3.9 | 4.3 | 3.7 | 4.5 | 5.8 | 6.6 | 6.5 | 11.2 | 10.8 | 10.3 | 5.9 | 6.9 | 7.3 |
Std Deviation (1e-4) | 0.8 | 1.5 | 1.1 | 1.1 | 1.8 | 1.2 | 1.2 | 1.1 | 0.8 | 0.8 | 0.8 | 1.0 | 1.2 | 1.7 | 2.1 | 2.4 | 2.5 | 2.5 | 1.4 | 2.0 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.03 | -0.4 | -0.49 | -0.36 | -0.64 | -0.48 | -0.69 | -0.43 | -0.07 | 0.13 | 0.12 | 0.89 | 0.84 | 0.77 | -0.04 | 0.21 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | 2.0 | 0.7 | 0.7 | 0.8 | 1.0 | -0.4 | 1.1 | -0.9 | 3.1 | 2.4 | 5.4 | 0.6 | 2.1 | 1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.4 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0.4 | 1.0 |
Nucleus | -7.8 | -5.7 | -7.5 | -9.0 | -9.0 | -8.7 | -7.8 | -9.5 | -7.5 | -7.5 | -10.1 | -12.4 | -8.6 | -9.8 | -9.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 7.8 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.7 | 10.5 | 9.5 | 8.7 | 8.8 | 9.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4.1 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.9 | 6.0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5699 | 1.6636 | 1.4191 | 1.1878 | 0.9356 | 1.4734 | 2.4888 | 2.0407 | 1.6432 | 1.2621 | 1.5633 | 1.8026 | 1.2027 | 1.6286 | 1.6625 | 1.6944 | 1.3534 | 1.4441 |
Actin | 0.0057 | 0 | 0.0011 | 0 | 0.0002 | 0.0002 | 0.0464 | 0.0001 | 0.0071 | 0.0342 | 0 | 0.0017 | 0.0068 | 0.0001 | 0.0039 | 0.0359 | 0.0282 | 0.0001 |
Bud | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 |
Bud Neck | 0.001 | 0.0005 | 0.0021 | 0.0003 | 0.0029 | 0.0009 | 0.0079 | 0.0005 | 0.0009 | 0.0042 | 0.0012 | 0.0014 | 0.0008 | 0.0005 | 0.0009 | 0.0006 | 0.0006 | 0.0016 |
Bud Periphery | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0003 |
Bud Site | 0.0005 | 0.0003 | 0.0024 | 0.0002 | 0.0002 | 0.0001 | 0.0013 | 0.0007 | 0.0013 | 0.0007 | 0.0001 | 0.0002 | 0.0011 | 0.0004 | 0.0005 | 0.0006 | 0.0003 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.0015 | 0.0002 | 0.0011 | 0.0001 | 0.0009 | 0.0001 | 0.0094 | 0.0001 | 0.0021 | 0.0003 | 0.0002 | 0.0016 | 0.0022 | 0.0002 | 0.0011 | 0.0005 | 0.0008 | 0.0034 |
Cytoplasmic Foci | 0.0024 | 0 | 0.0015 | 0.0001 | 0.0001 | 0.0002 | 0.0084 | 0.0001 | 0.0067 | 0.001 | 0 | 0.0057 | 0.0034 | 0 | 0.003 | 0.0018 | 0.0083 | 0.0003 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0009 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0053 | 0 | 0.0019 | 0.0004 | 0 | 0.0008 | 0.0013 | 0 | 0.0007 | 0.0015 | 0.0004 | 0.0001 |
Endosome | 0.0057 | 0.0003 | 0.0024 | 0.0001 | 0.0004 | 0.0009 | 0.0347 | 0.0003 | 0.008 | 0.0016 | 0.0001 | 0.0103 | 0.0086 | 0.0001 | 0.0035 | 0.0033 | 0.0053 | 0.0002 |
Golgi | 0.0013 | 0 | 0.0009 | 0 | 0 | 0.0001 | 0.0084 | 0.0001 | 0.003 | 0.0019 | 0 | 0.0007 | 0.0024 | 0 | 0.0015 | 0.0033 | 0.0078 | 0 |
Lipid Particles | 0.0077 | 0.0002 | 0.0081 | 0.0005 | 0.0001 | 0.0012 | 0.0105 | 0.0006 | 0.0064 | 0.0013 | 0 | 0.0019 | 0.0061 | 0.0002 | 0.0016 | 0.0087 | 0.0143 | 0.0002 |
Mitochondria | 0.0017 | 0.0004 | 0.0016 | 0.0003 | 0.0002 | 0.0002 | 0.0031 | 0.001 | 0.0038 | 0.0005 | 0.0005 | 0.0004 | 0.0095 | 0.0006 | 0.0013 | 0.002 | 0.0016 | 0.0002 |
None | 0.0014 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0064 | 0.0002 | 0.0029 | 0.0004 | 0.0003 | 0.0017 | 0.0036 | 0.0001 | 0.0006 | 0.0008 | 0.0005 | 0.0001 |
Nuclear Periphery | 0.0189 | 0.0066 | 0.0137 | 0.0009 | 0.0042 | 0.0073 | 0.0409 | 0.0073 | 0.0092 | 0.0027 | 0.0044 | 0.0156 | 0.0058 | 0.0035 | 0.005 | 0.0278 | 0.0048 | 0.0011 |
Nucleolus | 0.194 | 0.268 | 0.2859 | 0.2153 | 0.3031 | 0.2846 | 0.2306 | 0.3074 | 0.3086 | 0.2508 | 0.1074 | 0.3109 | 0.2268 | 0.3595 | 0.3869 | 0.2133 | 0.0704 | 0.3221 |
Nucleus | 0.7146 | 0.7171 | 0.6201 | 0.7515 | 0.6784 | 0.6885 | 0.5584 | 0.6719 | 0.617 | 0.6947 | 0.8802 | 0.6267 | 0.6972 | 0.63 | 0.5593 | 0.6591 | 0.8104 | 0.6534 |
Peroxisomes | 0.0019 | 0 | 0.0012 | 0.0001 | 0 | 0.0001 | 0.0027 | 0.0003 | 0.0083 | 0.0008 | 0 | 0.0005 | 0.0087 | 0 | 0.0037 | 0.0061 | 0.0168 | 0 |
Punctate Nuclear | 0.0374 | 0.0048 | 0.0514 | 0.0301 | 0.0083 | 0.0147 | 0.0201 | 0.0072 | 0.0098 | 0.0035 | 0.0046 | 0.0179 | 0.0126 | 0.0031 | 0.0234 | 0.032 | 0.0283 | 0.0157 |
Vacuole | 0.0015 | 0.0007 | 0.0028 | 0.0002 | 0.0003 | 0.0002 | 0.0021 | 0.0009 | 0.0016 | 0.0003 | 0.0004 | 0.0012 | 0.0014 | 0.0006 | 0.0015 | 0.0008 | 0.0005 | 0.0006 |
Vacuole Periphery | 0.0012 | 0.0003 | 0.0014 | 0.0001 | 0.0001 | 0.0003 | 0.0018 | 0.0005 | 0.0007 | 0.0001 | 0.0004 | 0.0006 | 0.0009 | 0.0005 | 0.0007 | 0.0011 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.6452 | 17.5616 | 10.8596 | 14.1196 | 19.1031 | 11.9987 | 11.5911 | 13.3161 | 8.5148 | 18.2776 |
Translational Efficiency | 1.6264 | 1.51 | 1.8782 | 1.1444 | 1.1501 | 1.8138 | 1.7445 | 0.814 | 1.4068 | 1.1037 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1585 | 1150 | 2954 | 1724 | 1041 | 1949 | 1430 | 1255 | 2626 | 3099 | 4384 | 2979 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 786.90 | 809.74 | 976.08 | 996.87 | 698.02 | 870.93 | 983.48 | 1020.62 | 751.67 | 848.22 | 978.49 | 1006.88 |
Standard Deviation | 101.44 | 153.34 | 140.39 | 146.94 | 113.49 | 116.85 | 132.88 | 159.66 | 114.92 | 134.86 | 138.03 | 152.88 |
Intensity Change Log 2 | — | 0.041278 | 0.310819 | 0.341225 | — | 0.319288 | 0.494627 | 0.548106 | — | 0.178651 | 0.400145 | 0.442176 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000099 | 0.000517 | 0.000403 | 0.000826 | 0.000249 | 0.000275 | 0.000455 | 0.001054 | 0.000158 | 0.000365 | 0.000420 | 0.000922 |
Bud Neck | 0.002992 | 0.010406 | 0.025414 | 0.040250 | 0.003783 | 0.008572 | 0.019238 | 0.052584 | 0.003306 | 0.009252 | 0.023400 | 0.045446 |
Bud Site | 0.000348 | 0.001702 | 0.000860 | 0.003937 | 0.000391 | 0.000748 | 0.000722 | 0.005074 | 0.000365 | 0.001102 | 0.000815 | 0.004416 |
Cell Periphery | 0.000096 | 0.000199 | 0.000087 | 0.000101 | 0.000123 | 0.000071 | 0.000045 | 0.000102 | 0.000107 | 0.000118 | 0.000073 | 0.000102 |
Cytoplasm | 0.004695 | 0.008591 | 0.000743 | 0.004599 | 0.006845 | 0.002274 | 0.000997 | 0.005354 | 0.005547 | 0.004618 | 0.000826 | 0.004917 |
Cytoplasmic Foci | 0.000452 | 0.001479 | 0.000006 | 0.000985 | 0.000642 | 0.001001 | 0.000029 | 0.002399 | 0.000528 | 0.001179 | 0.000014 | 0.001581 |
Eisosomes | 0.000012 | 0.000028 | 0.000030 | 0.000028 | 0.000020 | 0.000025 | 0.000023 | 0.000038 | 0.000015 | 0.000026 | 0.000028 | 0.000032 |
Endoplasmic Reticulum | 0.004851 | 0.006743 | 0.006216 | 0.007192 | 0.004092 | 0.005542 | 0.004832 | 0.007202 | 0.004550 | 0.005988 | 0.005765 | 0.007196 |
Endosome | 0.000386 | 0.001229 | 0.000249 | 0.001870 | 0.000493 | 0.000693 | 0.000214 | 0.003050 | 0.000429 | 0.000892 | 0.000238 | 0.002367 |
Golgi | 0.000036 | 0.001065 | 0.000031 | 0.001154 | 0.000122 | 0.000791 | 0.000052 | 0.002255 | 0.000070 | 0.000893 | 0.000038 | 0.001618 |
Lipid Particles | 0.000162 | 0.000575 | 0.000020 | 0.000121 | 0.000436 | 0.000558 | 0.000049 | 0.000333 | 0.000271 | 0.000564 | 0.000030 | 0.000210 |
Mitochondria | 0.001330 | 0.002628 | 0.001147 | 0.003934 | 0.000774 | 0.003149 | 0.000849 | 0.004032 | 0.001110 | 0.002956 | 0.001050 | 0.003975 |
Mitotic Spindle | 0.000176 | 0.001164 | 0.000279 | 0.017572 | 0.000315 | 0.004482 | 0.000558 | 0.022343 | 0.000231 | 0.003251 | 0.000370 | 0.019582 |
None | 0.003106 | 0.018611 | 0.000961 | 0.003230 | 0.010320 | 0.001549 | 0.001236 | 0.002439 | 0.005966 | 0.007880 | 0.001051 | 0.002897 |
Nuclear Periphery | 0.001239 | 0.000871 | 0.001185 | 0.004442 | 0.000968 | 0.001014 | 0.000569 | 0.003014 | 0.001131 | 0.000961 | 0.000984 | 0.003841 |
Nuclear Periphery Foci | 0.000173 | 0.000842 | 0.000090 | 0.001624 | 0.000646 | 0.000436 | 0.000116 | 0.001948 | 0.000360 | 0.000587 | 0.000099 | 0.001761 |
Nucleolus | 0.024345 | 0.067365 | 0.025316 | 0.081486 | 0.079989 | 0.061734 | 0.047152 | 0.134721 | 0.046403 | 0.063823 | 0.032439 | 0.103913 |
Nucleus | 0.953730* | 0.873599* | 0.935311* | 0.817449* | 0.888450* | 0.904866* | 0.921827* | 0.738669* | 0.927852* | 0.893263* | 0.930912* | 0.784260* |
Peroxisomes | 0.000141 | 0.000732 | 0.000086 | 0.000378 | 0.000335 | 0.000733 | 0.000184 | 0.001019 | 0.000218 | 0.000732 | 0.000118 | 0.000648 |
Vacuole | 0.001077 | 0.001119 | 0.001198 | 0.005569 | 0.000752 | 0.001086 | 0.000693 | 0.009008 | 0.000948 | 0.001098 | 0.001033 | 0.007018 |
Vacuole Periphery | 0.000555 | 0.000535 | 0.000367 | 0.003251 | 0.000255 | 0.000401 | 0.000159 | 0.003361 | 0.000436 | 0.000451 | 0.000299 | 0.003297 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.40 | -27.55* | -24.80* | -6.57 | -15.15 | -0.79 | -7.56 | -8.99 | -8.67 | -6.99 | -7.66 | -19.05 | -17.12 | -12.07 | -12.12 |
Bud Neck | -7.52 | -21.66 | -25.25 | -20.09 | -11.73 | -6.37 | -16.66 | -24.77 | -22.47 | -16.53 | -9.68 | -26.95 | -35.19 | -30.43 | -19.46 |
Bud Site | -4.68 | -4.47 | -7.13 | -4.29 | -6.07 | -2.81 | -4.50 | -12.19 | -11.47 | -11.13 | -5.41 | -6.00 | -12.88 | -10.71 | -11.41 |
Cell Periphery | -2.32 | 0.65 | -0.12 | 2.33 | -0.97 | 2.16 | 4.05 | 1.03 | -1.80 | -5.67 | -0.64 | 2.77 | 0.57 | 1.02 | -3.15 |
Cytoplasm | -2.90 | 5.31 | -0.57 | 2.54 | -6.42 | 3.42 | 4.39 | 1.00 | -3.19 | -4.65 | 0.88 | 6.93 | 0.25 | -0.72 | -8.12 |
Cytoplasmic Foci | -2.15 | 1.95 | -0.67 | 1.77 | -3.00 | -1.18 | 3.76 | -2.19 | -1.53 | -3.12 | -2.41 | 3.38 | -2.23 | -0.12 | -4.06 |
Eisosomes | -9.14 | -14.82 | -15.93 | -0.06 | -0.25 | -2.15 | -4.06 | -11.76 | -5.75 | -9.35 | -7.05 | -14.32 | -19.40 | -4.25 | -5.87 |
Endoplasmic Reticulum | -3.56 | -3.05 | -3.97 | -0.03 | -1.76 | -3.49 | -2.42 | -5.15 | -2.29 | -3.85 | -4.15 | -4.08 | -6.21 | -2.20 | -3.56 |
Endosome | -3.07 | 1.41 | -3.41 | -0.50 | -3.81 | -1.29 | 3.03 | -3.98 | -3.48 | -4.55 | -3.37 | 2.85 | -5.25 | -3.19 | -5.92 |
Golgi | -2.24 | -0.53 | -2.92 | 0.38 | -2.91 | -1.96 | 1.34 | -2.54 | -1.24 | -2.67 | -3.05 | 1.33 | -3.76 | -0.72 | -3.86 |
Lipid Particles | -2.19 | 4.91 | 0.40 | 2.26 | -10.37 | -0.42 | 2.44 | 0.51 | 0.79 | -5.20 | -1.63 | 3.67 | 0.51 | 1.90 | -7.96 |
Mitochondria | -1.74 | 0.17 | -4.51 | -2.15 | -8.53 | -3.25 | -2.43 | -11.79 | -1.43 | -11.12 | -3.14 | -0.15 | -7.91 | -2.16 | -13.07 |
Mitotic Spindle | -3.13 | -1.14 | -7.47 | -6.95 | -7.40 | -3.62 | -1.27 | -7.72 | -6.03 | -7.33 | -4.21 | -1.54 | -10.73 | -8.55 | -10.49 |
None | -5.85 | 3.93 | -0.22 | 5.76 | -3.78 | 5.16 | 5.34 | 4.53 | -3.34 | -4.84 | -1.62 | 6.53 | 3.60 | 4.65 | -5.21 |
Nuclear Periphery | 1.76 | -0.14 | -5.90 | -6.67 | -5.99 | -0.36 | 2.10 | -7.15 | -7.17 | -8.42 | 1.16 | 0.43 | -8.35 | -9.01 | -8.73 |
Nuclear Periphery Foci | -3.77 | 0.64 | -2.58 | -0.75 | -2.67 | 0.99 | 2.69 | -2.80 | -3.45 | -4.28 | -2.19 | 2.67 | -3.76 | -2.79 | -4.54 |
Nucleolus | -10.85 | -1.08 | -14.67 | -4.00 | -14.34 | 3.70 | 4.97 | -8.87 | -12.63 | -13.33 | -6.00 | 4.31 | -16.51 | -11.80 | -20.34 |
Nucleus | 13.36 | 6.73 | 24.58 | 9.44 | 21.68 | -2.57 | -3.38 | 19.97 | 22.98 | 23.52 | 8.83 | 1.23 | 31.35 | 23.46 | 32.26 |
Peroxisomes | -5.11 | 1.75 | -5.75 | 2.57 | -7.57 | -1.92 | 2.21 | -5.34 | -2.15 | -6.29 | -3.85 | 3.12 | -7.10 | -0.38 | -8.56 |
Vacuole | -0.46 | -0.54 | -6.05 | -6.50 | -6.49 | -1.67 | -0.74 | -8.03 | -7.40 | -7.93 | -1.06 | -0.80 | -9.87 | -9.70 | -10.13 |
Vacuole Periphery | 0.05 | 0.83 | -3.06 | -3.22 | -3.48 | -2.03 | 2.61 | -4.61 | -4.30 | -4.79 | -0.16 | 0.97 | -4.94 | -5.06 | -5.42 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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