Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0.05 – 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0.05 0 0.05 – 0.15 0 0 0 0 0 0 0
Golgi 0 0.07 0.22 0.16 0.28 0 0 0 0 0 0 0 0.15 0.23 0.32 – 0.25 0.39 0.05 0.09 0.07 0.07 0.1 0.1
Mitochondria 0.93 0.95 0.79 0.89 0.68 0.85 0.76 0.8 0.91 0.9 0.91 0.91 0.79 0.87 0.8 – 0.05 0.11 0.84 0.79 0.81 0.77 0.67 0.71
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0.05 0.09 0.05 0 0.07 0.14 0.11 0.09 0.1 0.05 0.07 0.09 0 0.05 – 0.15 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0.06 0.05 0.11 0.08 0.09 0.07 0.09 0.07 0 0 0 – 0.1 0.14 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 – 0.15 0.11 0.05 0 0.06 0 0 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.16 0.1 0.07 0.19 0.1 0.09 0.07 0.05 0 0 0 0.05 0.09 0.08 – 0.1 0.19 0 0.05 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 4 2 3 2 0 3 0 0 1 0 0 2 0 – 0 0 2 1 1 0 5 2
Bud 1 2 2 3 1 3 7 6 9 16 12 7 1 1 1 – 0 1 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 4 4 1 0 0 0 0 – 0 1 – – – – – –
Cell Periphery 4 11 8 7 4 5 4 4 1 5 0 0 4 4 4 – 1 2 0 0 0 0 0 0
Cytoplasm 0 5 6 11 15 8 11 6 6 6 7 8 4 7 12 – 0 3 0 1 0 0 3 0
Endoplasmic Reticulum 1 2 0 6 1 0 2 4 4 0 3 1 0 1 1 – 1 0 0 1 0 1 0 1
Endosome 2 0 7 1 15 14 16 16 4 3 3 1 6 5 11 – 3 1 2 2 5 5 6 3
Golgi 3 14 48 33 73 12 4 15 2 2 1 1 18 49 77 – 5 14 9 17 16 9 16 20
Mitochondria 190 187 170 185 181 236 290 291 371 381 278 302 93 183 194 – 1 4 175 159 192 100 110 149
Nucleus 0 0 1 1 1 1 4 1 2 4 2 3 0 0 0 – 1 1 0 0 0 0 0 1
Nuclear Periphery 12 9 19 11 11 20 55 39 37 43 14 24 10 5 11 – 3 0 0 0 0 2 2 1
Nucleolus 18 2 4 4 17 13 44 29 37 30 29 23 0 2 2 – 2 5 1 0 0 0 3 2
Peroxisomes 3 1 11 1 1 4 7 7 2 7 1 5 4 7 11 – 3 4 10 4 14 3 5 9
SpindlePole 0 0 2 0 6 4 2 6 4 10 2 4 0 1 1 – 0 2 1 1 0 0 0 0
Vac/Vac Membrane 23 31 22 14 51 29 35 24 20 14 13 9 6 19 20 – 2 7 2 9 1 5 7 12
Unique Cell Count 204 197 216 207 265 277 384 362 409 423 306 331 117 211 243 20 36 208 201 237 131 165 211
Labelled Cell Count 257 267 304 279 380 351 481 451 503 525 367 388 146 286 345 22 45 208 201 237 131 165 211


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 8.2 10.3 9.5 10.4 9.0 8.5 8.6 7.2 7.6 7.5 7.3 9.7 10.3 9.8 – 10.8 11.1 10.5 11.5 11.3
Std Deviation (1e-4) 2.0 1.8 2.0 1.8 2.0 1.7 1.8 1.7 1.7 1.8 2.0 1.8 1.7 2.0 1.6 – 2.3 3.2 1.9 2.3 2.1
Intensity Change (Log2) – – – -0.11 0.01 -0.2 -0.28 -0.26 -0.51 -0.45 -0.47 -0.5 -0.09 0.0 -0.07 – 0.06 0.11 0.03 0.16 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 0 2.1 2.1 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery -0.2 -1.5 -1.3 0 0 0 0 0 0 0 -1.1 -1.4 – 0 0
Cytoplasm 1.3 1.5 0.1 0.1 0 0 0 -0.4 -0.3 0 0.3 1.2 – 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Endosome 0 1.3 1.0 0.6 0.7 0 0 0 0 0 -0.5 0.7 – 0 0
Golgi -1.6 1.3 -6.0 -8.9 -6.7 -9.5 -9.7 -8.4 -8.8 -1.5 0.2 2.3 – 0 2.1
Mitochondria 3.0 -2.6 1.9 -0.9 0.5 4.2 3.9 3.9 4.2 0.2 2.2 0.3 – -7.1 -8.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery -1.4 -2.1 -0.6 2.0 0.8 0.1 0.6 -2.0 -0.7 -0.1 -2.9 -1.8 – 0 0
Nucleolus 0 2.4 1.7 4.2 3.1 3.5 2.8 3.5 2.7 0 0 0 – 0 0
Peroxisomes -2.9 -3.3 -2.3 -2.3 -2.1 0 -2.5 0 -2.4 -0.7 -0.9 -0.3 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole -1.3 2.8 0.1 -0.4 -1.5 -2.5 -3.6 -2.7 -3.7 -1.6 -0.4 -0.7 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.2734 7.2999 6.8912 7.0068 5.647 7.4149 11.5781 11.3466 11.3889 10.9977 9.7789 11.0713 6.7743 8.0286 7.2469 8.0493 7.4666 7.4785
Actin 0.0578 0.004 0.0263 0 0.0648 0.0061 0.0159 0.0033 0.0058 0.0009 0.0201 0.0037 0.0399 0.0009 0.0459 0.0005 0.0007 0.0005
Bud 0.0005 0.0001 0.0001 0 0.0009 0.0001 0.0001 0.0001 0 0.0002 0.0002 0 0.0006 0.0001 0.0003 0.0008 0.0001 0
Bud Neck 0.0026 0.0001 0.0004 0 0.0028 0.0005 0.0001 0 0 0 0.0016 0 0.001 0 0.0012 0 0 0
Bud Periphery 0.0008 0.0002 0.0003 0 0.0015 0.0002 0.0003 0.0002 0.0001 0.0012 0.0005 0 0.0013 0.0001 0.0005 0.0039 0.0002 0.0001
Bud Site 0.0028 0.0002 0.0008 0 0.0026 0.0001 0.0006 0.0002 0.0001 0 0.0008 0 0.007 0.0001 0.0035 0.0001 0.0001 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0 0.0003 0.0001 0.0002 0.0001 0.0001 0 0.0003 0.0001 0.0004 0.0001 0.0001 0.0004 0 0.0001
Cytoplasm 0.0049 0 0 0 0.0022 0.0001 0.0011 0 0 0 0.0002 0 0.0141 0 0.0006 0 0 0.0001
Cytoplasmic Foci 0.0125 0.0024 0.0015 0 0.0446 0.0017 0.0034 0.0012 0.0006 0 0.002 0.0004 0.0176 0.0008 0.0078 0.0001 0.0002 0.0012
Eisosomes 0.0007 0.0001 0.0004 0 0.0009 0.0001 0.0004 0.0001 0.0001 0.0001 0.0016 0.0002 0.0009 0.0001 0.0005 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0029 0.0001 0.0001 0 0.0027 0.0005 0.0003 0.0001 0.0001 0 0.0012 0 0.0037 0.0001 0.0011 0 0 0.0002
Endosome 0.0233 0.0073 0.0038 0.0001 0.024 0.008 0.0042 0.0061 0.0039 0.0002 0.0056 0.0009 0.0338 0.0113 0.0078 0.0004 0.0006 0.0082
Golgi 0.0207 0.0323 0.0139 0.0003 0.0489 0.0123 0.0081 0.0157 0.0079 0.0006 0.0089 0.0032 0.0177 0.0101 0.0084 0.0008 0.0011 0.0092
Lipid Particles 0.0286 0.0149 0.0032 0.0001 0.0781 0.0189 0.0094 0.002 0.0021 0.0008 0.0115 0.0037 0.0334 0.0037 0.0091 0.0004 0.0005 0.006
Mitochondria 0.7727 0.9154 0.9416 0.9988 0.6554 0.923 0.9272 0.9638 0.9715 0.995 0.9304 0.9829 0.7699 0.9635 0.8951 0.9847 0.9703 0.9689
None 0.0029 0 0.0001 0 0.0018 0.0002 0.0007 0 0 0 0.0027 0 0.0115 0 0.0004 0 0 0.0001
Nuclear Periphery 0.0109 0.0001 0.0001 0 0.0043 0.0032 0.0027 0 0.0001 0 0.0038 0.0001 0.004 0.0002 0.0018 0 0.0001 0.0003
Nucleolus 0.009 0.0001 0 0 0.0008 0.0002 0.0006 0.0001 0 0 0.0007 0 0.0007 0.0001 0.0004 0.0001 0 0.0001
Nucleus 0.0062 0.0001 0 0 0.0011 0.0003 0.0012 0 0 0 0.0008 0 0.003 0 0.0008 0.0001 0 0.0001
Peroxisomes 0.0247 0.013 0.0057 0.0001 0.0181 0.0172 0.0182 0.0049 0.0028 0.0004 0.004 0.0039 0.0251 0.0044 0.0076 0.003 0.0207 0.0012
Punctate Nuclear 0.0048 0 0.0001 0 0.0396 0.0025 0.0005 0 0 0 0.0016 0 0.0057 0 0.0044 0 0 0.0001
Vacuole 0.0044 0.0024 0.0003 0 0.0013 0.0008 0.0015 0.0005 0.0009 0.0001 0.0005 0.0001 0.0038 0.0012 0.0011 0.0021 0.0005 0.0007
Vacuole Periphery 0.0062 0.007 0.0011 0.0005 0.0031 0.0041 0.0032 0.0015 0.0038 0.0004 0.001 0.0005 0.0049 0.003 0.0015 0.0026 0.0048 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9612 32.2588 11.0481 20.512 17.9951 23.5817 28.8534 29.2111 33.1478 24.7932
Translational Efficiency 1.9597 0.9564 2.6665 1.8551 1.6902 1.5954 1.4638 1.8795 1.5519 1.5954

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
75 351 325 1236 1371 2224 969 1297 1446 2575 1294 2533

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1144.35 1061.49 1381.37 1282.50 896.26 1162.11 1232.36 1402.38 909.13 1148.39 1269.79 1343.88
Standard Deviation 211.65 182.53 217.53 205.87 158.67 176.73 207.20 232.70 170.94 180.86 219.57 228.03
Intensity Change Log 2 — -0.108438 0.271571 0.164430 — 0.374757 0.459435 0.645888 — 0.123897 0.357110 0.395857

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003977 0.005604 0.014372 0.002967 0.002382 0.002187 0.003565 0.006814 0.002465 0.002653 0.006280 0.004937
Bud Neck 0.002090 0.002089 0.007332 0.001362 0.001479 0.000637 0.002482 0.001046 0.001510 0.000835 0.003700 0.001200
Bud Site 0.008360 0.004111 0.006238 0.003645 0.002932 0.000962 0.004262 0.001604 0.003214 0.001391 0.004758 0.002600
Cell Periphery 0.001060 0.000157 0.000186 0.000273 0.000278 0.000097 0.000120 0.000172 0.000319 0.000105 0.000137 0.000221
Cytoplasm 0.021633 0.001094 0.000712 0.000761 0.000318 0.000280 0.000807 0.000364 0.001424 0.000391 0.000783 0.000558
Cytoplasmic Foci 0.024695 0.023453 0.045378 0.009715 0.011224 0.011210 0.026579 0.011073 0.011922 0.012879 0.031300 0.010410
Eisosomes 0.000122 0.000211 0.000252 0.000148 0.000120 0.000097 0.000121 0.000306 0.000120 0.000113 0.000154 0.000229
Endoplasmic Reticulum 0.017026 0.000199 0.000826 0.000436 0.000239 0.000106 0.000263 0.000160 0.001109 0.000118 0.000404 0.000295
Endosome 0.027008 0.023872 0.025036 0.008995 0.008691 0.009772 0.013868 0.004474 0.009641 0.011694 0.016673 0.006680
Golgi 0.080824 0.037156 0.083698 0.033020 0.015742 0.022336 0.041200 0.036464 0.019117 0.024356 0.051874 0.034783
Lipid Particles 0.019638 0.006679 0.006051 0.001747 0.008201 0.004058 0.005250 0.002053 0.008794 0.004415 0.005451 0.001904
Mitochondria 0.648896* 0.825350* 0.731595* 0.895642* 0.921134* 0.930209* 0.851436* 0.914469* 0.907014* 0.915915* 0.821337* 0.905282*
Mitotic Spindle 0.013688 0.001919 0.021098 0.004602 0.001193 0.000887 0.006558 0.002777 0.001841 0.001028 0.010210 0.003668
None 0.004151 0.000502 0.000991 0.000921 0.000628 0.000315 0.000688 0.000874 0.000811 0.000341 0.000764 0.000897
Nuclear Periphery 0.002395 0.000191 0.002059 0.000641 0.000152 0.000099 0.001058 0.000133 0.000268 0.000111 0.001309 0.000381
Nuclear Periphery Foci 0.019330 0.001362 0.001139 0.000337 0.000447 0.000162 0.000549 0.000123 0.001426 0.000326 0.000697 0.000227
Nucleolus 0.011477 0.001280 0.000231 0.000380 0.000417 0.000444 0.000265 0.000152 0.000991 0.000558 0.000257 0.000263
Nucleus 0.036269 0.000339 0.000139 0.000054 0.000196 0.000135 0.000097 0.000035 0.002067 0.000163 0.000108 0.000044
Peroxisomes 0.009710 0.058492 0.027749 0.009153 0.018459 0.010882 0.019707 0.010335 0.018005 0.017372 0.021727 0.009758
Vacuole 0.033587 0.003550 0.010997 0.010681 0.002981 0.002936 0.008156 0.002544 0.004568 0.003020 0.008870 0.006515
Vacuole Periphery 0.014066 0.002389 0.013923 0.014519 0.002787 0.002189 0.012969 0.004028 0.003372 0.002216 0.013209 0.009147

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.80 -3.08 1.06 1.82 3.61 0.28 -1.51 -3.25 -3.66 -2.47 -0.25 -3.62 -2.79 -3.05 1.22
Bud Neck 0.07 -2.53 1.06 1.58 3.07 2.36 -2.04 1.16 -1.93 3.43 1.99 -3.41 0.90 -2.11 4.32
Bud Site 1.05 0.52 1.21 0.24 1.33 2.35 -1.11 1.42 -1.30 2.47 2.17 -1.38 0.63 -2.16 2.20
Cell Periphery 1.83 1.77 1.58 -1.79 -1.37 1.85 1.61 1.00 -1.77 -1.24 2.22 1.89 0.94 -3.06 -2.26
Cytoplasm 3.04 3.10 3.09 0.62 -0.22 0.47 -2.16 -0.64 -0.93 1.92 2.73 1.70 2.28 -1.19 1.07
Cytoplasmic Foci 0.31 -3.07 3.49 4.33 6.79 0.02 -5.96 0.09 0.09 6.07 -0.65 -7.95 1.10 2.04 9.04
Eisosomes -2.93 -3.44 -1.13 2.32 2.96 1.80 -0.07 -5.23 -5.97 -5.24 0.64 -2.32 -5.21 -5.82 -3.49
Endoplasmic Reticulum 2.28 2.20 2.25 -2.58 1.17 2.63 -0.38 1.53 -2.50 2.46 2.50 1.76 2.05 -4.06 1.13
Endosome 0.42 0.26 2.79 4.14 5.09 -0.84 -3.37 3.79 5.46 7.26 -1.57 -4.62 2.52 4.77 7.61
Golgi 2.23 -0.22 2.48 0.80 5.97 -3.04 -7.20 -6.38 -4.29 1.10 -2.33 -9.10 -5.80 -4.08 4.51
Lipid Particles 2.10 2.21 2.87 3.27 3.95 3.01 1.88 4.73 2.68 3.02 3.31 2.37 5.56 3.92 4.31
Mitochondria -3.86 -1.93 -5.51 -4.09 -8.45 -1.40 7.10 0.89 2.46 -6.47 -1.37 8.70 0.16 1.90 -9.47
Mitotic Spindle 1.92 -0.64 1.51 -2.08 3.39 0.91 -5.25 -1.52 -1.83 2.64 1.86 -5.64 -1.94 -3.29 4.03
None 1.96 1.75 1.78 -5.44 0.73 5.37 -0.83 -2.85 -8.31 -2.32 3.86 0.67 -0.29 -11.80 -2.24
Nuclear Periphery 2.72 0.37 2.10 -1.67 2.09 1.69 -3.40 0.72 -1.25 3.48 2.92 -4.01 -0.84 -2.18 3.29
Nuclear Periphery Foci 2.59 2.63 2.73 1.15 2.60 1.89 -0.65 2.24 0.54 4.96 2.66 1.92 2.96 0.69 4.54
Nucleolus 2.08 2.31 2.28 1.00 -0.87 -0.10 1.26 2.20 1.08 1.41 1.22 2.60 2.47 1.06 -0.10
Nucleus 3.09 3.10 3.11 1.68 2.47 0.77 1.69 3.07 1.70 2.49 2.97 3.06 3.15 2.10 3.01
Peroxisomes -5.21 -2.47 0.26 5.88 3.15 3.08 -0.40 2.96 0.26 3.19 0.26 -1.22 3.49 3.84 4.64
Vacuole 2.77 2.07 2.11 -4.45 0.12 0.06 -4.90 0.64 0.61 5.74 1.73 -3.73 -1.77 -4.11 2.16
Vacuole Periphery 3.50 -0.00 -0.17 -7.96 -0.27 1.34 -6.54 -2.08 -3.88 5.71 2.50 -7.70 -6.78 -9.13 2.87
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Rsm25

Rsm25


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsm25-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available