ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.99 0.99 0.99 0.99 0.94 0.92 0.91 0.74 0.83 0.53 0.7 0.99 0.99 1.0 0.93 0.91 0.91 0.86 0.87 0.68 0.91 0.89 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0 0.05 0.08 0.05 0.28 0.2 0.64 0.32 0 0 0 0.08 0.08 0.06 0 0.05 0.16 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.05 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 5
Bud 0 0 0 0 0 0 2 2 1 1 7 7 0 0 0 0 0 0 0 2 0 1 3 8
Bud Neck 0 1 4 1 9 2 5 12 3 3 0 3 0 1 1 0 1 0 0 1 0 1 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 2 2 2 2 3 2 0 0 1 1 3 1 1 2 0 0 0 0 1 2
Cytoplasm 102 310 135 217 254 203 291 409 180 239 97 177 191 261 283 160 144 164 94 276 89 136 285 302
Endoplasmic Reticulum 0 0 0 1 0 0 1 2 1 3 0 0 1 0 0 15 8 11 0 0 1 0 0 0
Endosome 0 1 0 0 0 4 2 4 7 3 0 2 0 2 0 6 4 4 0 3 2 0 1 4
Golgi 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 0 2 0 0 0 1
Mitochondria 10 1 0 4 0 10 25 21 67 59 117 82 0 0 0 14 12 10 4 15 20 3 6 8
Nucleus 0 0 2 2 4 2 3 5 3 3 5 4 0 1 0 0 0 1 0 0 0 1 2 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 10 16 10 10 7 8 0 0 0 1 1 0 0 2 1 0 5 6
Vac/Vac Membrane 0 3 1 1 0 1 1 4 3 8 1 4 5 0 0 9 11 9 5 5 4 0 3 2
Unique Cell Count 108 313 137 219 257 215 318 448 242 289 183 254 193 264 283 172 158 180 110 319 132 149 323 364
Labelled Cell Count 112 316 142 227 269 225 343 477 279 332 236 288 198 267 287 207 182 201 110 319 132 149 323 364


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.0 5.2 5.0 5.1 4.4 4.5 4.9 3.9 4.2 3.5 3.7 5.1 5.2 5.0 5.6 5.6 5.3 5.1 4.9 5.3
Std Deviation (1e-4) 0.6 0.6 1.6 1.8 1.6 1.8 1.8 2.1 1.4 2.0 1.4 1.4 1.3 1.2 1.3 1.7 1.6 1.8 1.3 1.3 1.6
Intensity Change (Log2) – – – -0.08 -0.03 -0.26 -0.23 -0.08 -0.43 -0.3 -0.59 -0.48 -0.02 -0.01 -0.07 0.1 0.09 0.03 -0.03 -0.1 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 2.7 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1034 -0.5565 -0.4712 -0.5056 -0.1564 -0.5989 -0.8029 -0.4965 -0.5819 -0.9535 -0.4902 -0.6752 -0.9802 -0.8232 -0.8636 -0.1712 -0.8392 -1.2107
Actin 0.0687 0.0002 0.0169 0.0044 0.0109 0.0068 0.027 0 0.008 0.0195 0.0568 0.0023 0.025 0.011 0.02 0.0793 0.023 0.0069
Bud 0.0005 0.0085 0.0136 0.0001 0.0007 0.0001 0.0012 0 0.0005 0 0.0005 0.0014 0.0004 0.0001 0.0004 0.0007 0.0026 0.0002
Bud Neck 0.0015 0.0002 0.0029 0.0001 0.0002 0.0004 0.001 0 0.0003 0.0001 0.001 0.0022 0.0019 0.0001 0.0008 0.0011 0.0082 0.0007
Bud Periphery 0.0007 0.0032 0.0057 0.0002 0.0012 0.0001 0.0032 0 0.0008 0 0.0006 0.0037 0.0004 0 0.0005 0.0011 0.0041 0.0004
Bud Site 0.0043 0.0166 0.0165 0.0002 0.0015 0.0003 0.0088 0.0001 0.0025 0.0001 0.0026 0.0004 0.005 0.0008 0.0058 0.0044 0.0605 0.0005
Cell Periphery 0.0002 0.0004 0.0006 0 0.0003 0 0.0008 0 0.0002 0 0.0002 0.0001 0.0002 0 0.0002 0.0002 0.0009 0
Cytoplasm 0.0712 0.1101 0.0595 0.081 0.0378 0.1698 0.0242 0.0235 0.0294 0.0442 0.0944 0.0635 0.0739 0.1062 0.041 0.0282 0.0265 0.1504
Cytoplasmic Foci 0.0331 0.0139 0.0098 0.0119 0.023 0.0143 0.024 0.0014 0.0062 0.0066 0.0221 0.0035 0.0266 0.0023 0.033 0.0304 0.017 0.0169
Eisosomes 0.0013 0.0001 0.0006 0.0001 0.0004 0.0001 0.0007 0.0001 0.0003 0.0002 0.0006 0.0001 0.0005 0.0001 0.0004 0.0007 0.0006 0.0001
Endoplasmic Reticulum 0.0067 0.0012 0.0009 0.0009 0.0212 0.0016 0.0029 0.0002 0.0017 0.001 0.0045 0.0009 0.0039 0.0009 0.0012 0.0035 0.0012 0.001
Endosome 0.0168 0.0006 0.0033 0.0067 0.0972 0.0072 0.0142 0 0.0061 0.001 0.0327 0.0046 0.0158 0.0008 0.0138 0.0146 0.0226 0.0028
Golgi 0.0052 0.0001 0.0018 0.0055 0.0114 0.005 0.0035 0 0.001 0.0023 0.0184 0.0064 0.0089 0.0001 0.0055 0.0222 0.0394 0.0035
Lipid Particles 0.013 0.0006 0.0022 0.0431 0.0327 0.0045 0.0079 0.0001 0.0005 0.0069 0.0457 0.0005 0.0239 0.0001 0.0132 0.0388 0.0151 0.0017
Mitochondria 0.0092 0.0005 0.0013 0.0874 0.0167 0.0036 0.016 0 0.0016 0.0009 0.0094 0.0027 0.0033 0.0001 0.0036 0.0066 0.0338 0.0209
None 0.6573 0.8356 0.8558 0.7133 0.668 0.7738 0.8237 0.9728 0.9328 0.8588 0.6743 0.8811 0.7499 0.8654 0.823 0.7208 0.6953 0.768
Nuclear Periphery 0.0295 0.0009 0.0006 0.0006 0.0433 0.001 0.0128 0.0001 0.0011 0.0007 0.0045 0.0108 0.0085 0.0006 0.0014 0.0085 0.004 0.001
Nucleolus 0.0037 0.0007 0.0011 0.0001 0.0018 0.0001 0.0008 0.0001 0.0011 0.0001 0.0021 0.0007 0.0007 0.0001 0.0029 0.0009 0.0049 0.0003
Nucleus 0.0417 0.0026 0.0008 0.0004 0.0072 0.004 0.0025 0.0007 0.0011 0.0083 0.005 0.006 0.0029 0.0102 0.001 0.0041 0.0101 0.0146
Peroxisomes 0.0086 0.0002 0.0019 0.0413 0.0024 0.0043 0.0093 0.0001 0.0006 0.0479 0.0155 0.0016 0.0347 0.0001 0.0186 0.019 0.0136 0.0039
Punctate Nuclear 0.0204 0.0029 0.0016 0.0011 0.0107 0.001 0.0038 0.0005 0.0015 0.0012 0.0043 0.0063 0.0117 0.0005 0.0129 0.0133 0.0116 0.0053
Vacuole 0.0034 0.0007 0.0023 0.0009 0.0057 0.0016 0.0097 0.0001 0.0021 0.0001 0.0036 0.0007 0.0012 0.0005 0.0007 0.001 0.0038 0.0004
Vacuole Periphery 0.0029 0.0001 0.0003 0.0007 0.0055 0.0002 0.002 0 0.0004 0.0001 0.0012 0.0006 0.0008 0 0.0002 0.0006 0.0013 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.1907 5.186 5.0027 5.238 1.5043 1.7394 6.2923 4.0699 7.297 5.0273
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
192 1362 390 642 1938 2245 1525 387 2130 3607 1915 1029

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 636.02 695.81 883.99 831.51 661.67 689.31 829.23 826.38 659.36 691.76 840.38 829.58
Standard Deviation 64.89 105.10 109.88 105.38 69.44 88.68 102.22 111.57 69.43 95.27 106.14 107.78
Intensity Change Log 2 — 0.129621 0.474958 0.386661 — 0.059041 0.325660 0.320693 — 0.094065 0.400765 0.353403

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000059 0.000273 0.000362 0.000859 0.000069 0.000456 0.000151 0.000817 0.000068 0.000387 0.000194 0.000843
Bud Neck 0.003094 0.025333 0.004574 0.017101 0.012968 0.038310 0.003942 0.006610 0.012078 0.033410 0.004071 0.013156
Bud Site 0.000418 0.009956 0.001680 0.028962 0.002544 0.013816 0.009416 0.028006 0.002353 0.012358 0.007841 0.028603
Cell Periphery 0.000195 0.000143 0.000184 0.000229 0.000131 0.000209 0.000176 0.000307 0.000137 0.000184 0.000177 0.000258
Cytoplasm 0.367510* 0.364675* 0.119455* 0.355950* 0.482781* 0.367613* 0.376978* 0.369320* 0.472390* 0.366504* 0.324532* 0.360978*
Cytoplasmic Foci 0.125351 0.177080 0.011497 0.017603 0.156171 0.192067* 0.013033 0.028607 0.153393 0.186408* 0.012720 0.021741
Eisosomes 0.000125 0.000114 0.000073 0.000057 0.000091 0.000126 0.000041 0.000059 0.000094 0.000122 0.000048 0.000058
Endoplasmic Reticulum 0.000369 0.001057 0.005627 0.004595 0.000557 0.000792 0.001339 0.001865 0.000540 0.000892 0.002213 0.003568
Endosome 0.001959 0.012390 0.002205 0.013609 0.004802 0.014251 0.001644 0.026793 0.004546 0.013549 0.001758 0.018567
Golgi 0.000326 0.002285 0.000078 0.002842 0.000808 0.004785 0.000090 0.011562 0.000765 0.003841 0.000088 0.006122
Lipid Particles 0.005391 0.007269 0.002607 0.005916 0.005248 0.009749 0.001200 0.002647 0.005261 0.008812 0.001487 0.004686
Mitochondria 0.000542 0.007663 0.000616 0.003272 0.003666 0.017027 0.001109 0.006445 0.003384 0.013491 0.001009 0.004465
Mitotic Spindle 0.001078 0.004384 0.001417 0.039905 0.000698 0.009768 0.015787 0.060113 0.000732 0.007735 0.012861 0.047505
None 0.005432 0.005115 0.008676 0.010330 0.003263 0.004372 0.009246 0.006509 0.003458 0.004652 0.009130 0.008893
Nuclear Periphery 0.000450 0.001111 0.001952 0.002215 0.000529 0.000890 0.001582 0.001547 0.000522 0.000973 0.001658 0.001964
Nuclear Periphery Foci 0.000716 0.000435 0.000681 0.001360 0.000174 0.000255 0.000320 0.001475 0.000222 0.000323 0.000394 0.001403
Nucleolus 0.001199 0.003084 0.001571 0.000725 0.000962 0.002288 0.000824 0.000634 0.000983 0.002589 0.000976 0.000691
Nucleus 0.470526* 0.317155* 0.802414* 0.399980* 0.296876* 0.255842* 0.526956* 0.335874* 0.312529* 0.278993* 0.583054* 0.375870*
Peroxisomes 0.001078 0.012253 0.000351 0.000663 0.003822 0.015338 0.000247 0.004596 0.003575 0.014173 0.000268 0.002142
Vacuole 0.014029 0.047101 0.033795 0.092793 0.023419 0.049367 0.035716 0.099131 0.022573 0.048512 0.035324 0.095177
Vacuole Periphery 0.000155 0.001122 0.000186 0.001036 0.000421 0.002678 0.000203 0.007080 0.000397 0.002090 0.000199 0.003309

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.18 -8.59 -3.81 -2.34 0.11 -3.98 -4.92 -4.13 -1.75 -3.37 -5.16 -8.91 -5.59 -2.38 -3.55
Bud Neck -9.90 -2.47 -4.54 4.12 -1.86 -10.86 6.61 3.56 13.49 -2.28 -11.84 5.51 0.44 11.17 -4.00
Bud Site -6.39 -4.55 -6.00 -1.82 -4.51 -8.41 -3.47 -3.79 -0.93 -2.31 -9.62 -3.74 -6.33 -1.59 -3.70
Cell Periphery 1.85 0.43 1.90 -0.02 2.10 -3.47 -1.11 -1.08 0.36 -0.71 -2.53 -1.30 -0.95 0.93 -0.12
Cytoplasm 0.36 13.97 5.31 8.12 -12.76 14.07 17.86 13.24 5.73 2.34 14.86 23.71 20.52 10.39 0.50
Cytoplasmic Foci -4.50 11.90 11.70 29.88 -1.20 -5.99 33.34 28.96 36.06 -2.91 -6.29 35.28 33.92 48.56 -2.18
Eisosomes 1.04 3.53 4.12 4.45 0.45 -5.94 9.91 0.05 4.98 -7.35 -5.30 9.31 1.81 7.63 -8.36
Endoplasmic Reticulum -3.88 -11.94 -10.14 -8.79 4.25 -1.77 -10.25 -8.45 -8.19 -3.31 -2.90 -14.65 -12.28 -11.70 -2.54
Endosome -8.41 -1.17 -2.91 4.39 -2.52 -8.35 5.88 -2.74 0.83 -4.00 -10.17 5.47 -2.69 3.96 -5.10
Golgi -3.39 1.00 -1.00 2.32 -1.77 -6.23 4.58 -1.70 0.27 -2.08 -6.69 4.66 -1.60 1.97 -2.52
Lipid Particles -1.27 2.06 1.62 4.18 -0.19 -6.38 11.43 6.00 10.51 -3.82 -6.49 10.90 3.82 8.39 -2.04
Mitochondria -8.35 -2.18 -2.83 6.78 -2.11 -9.37 3.62 0.49 8.55 -1.84 -9.73 3.72 1.80 11.82 -2.07
Mitotic Spindle -2.14 -1.53 -6.62 -5.99 -5.91 -6.10 -6.66 -5.22 -3.91 -2.57 -6.76 -6.86 -8.56 -6.76 -4.49
None 0.27 -0.14 -1.44 -2.20 -0.76 -1.57 -5.16 -3.60 -2.11 0.82 -2.18 -4.58 -5.18 -3.21 -0.03
Nuclear Periphery -4.24 -15.05 -17.25 -12.17 -7.40 -4.24 -15.73 -9.66 -7.63 -1.25 -5.70 -19.11 -19.39 -15.29 -6.91
Nuclear Periphery Foci 0.37 -0.31 -2.40 -7.46 -5.38 -1.96 -6.04 -3.92 -3.78 -3.46 -1.84 -4.65 -7.74 -7.42 -6.66
Nucleolus -3.63 -0.99 -1.71 1.89 -1.27 -5.19 1.18 -1.38 2.13 -1.73 -6.35 0.27 -2.66 2.29 -2.73
Nucleus 6.90 -13.79 2.77 -6.00 23.74 5.33 -27.87 -4.09 -6.56 11.22 4.65 -33.68 -7.02 -10.44 18.62
Peroxisomes -9.95 3.12 2.14 10.58 -1.02 -11.11 7.71 2.23 11.48 -2.44 -12.65 7.80 4.96 17.30 -2.88
Vacuole -12.03 -7.41 -19.34 -15.81 -14.08 -11.55 -11.04 -14.65 -12.72 -11.83 -14.78 -12.60 -23.74 -20.41 -18.76
Vacuole Periphery -8.25 -1.58 -2.00 5.00 -1.43 -4.37 5.93 -1.96 -0.03 -2.18 -5.19 5.78 -1.92 1.51 -2.40
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
Localization
Cell Percentages cytoplasm (27%), nucleus (12%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YIL092W

YIL092W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yil092w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available