Standard name
Human Ortholog
Description Protein of unknown function; mitochondrial protein that physically interacts with Tim23p; null mutant displays reduced respiratory growth
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0.05 0 0 0.05 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.9 0.89 0.89 0.68 0.51 – 0.32 0.22 0.27 0.23 0.21 0.97 0.96 0.97 0.79 0.79 0.75 0.8 0.55 0.44 0.6 0.63 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.11 0.05 0.08 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.18 0.13 0.34 0.42 0.63 – 0.72 0.82 0.82 0.85 0.84 0 0 0 0 0 0.08 0.1 0.25 0.34 0.22 0.17 0.1
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0.07 0 0 0.05 0.07 0 0 0 0 0 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.05 0.05 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 1 1 2 0 3 3 3 5
Bud 0 0 3 1 3 3 – 11 9 11 11 15 1 2 1 1 2 0 1 1 0 0 9 3
Bud Neck 1 0 2 0 0 0 – 1 0 2 0 0 1 0 0 1 2 0 2 1 0 1 2 2
Bud Site 0 0 0 0 1 1 – 3 5 5 2 10 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 1 – 0 0 1 1 1 1 0 5 3 0 0 2 0 1 1 0 5
Cytoplasm 252 60 108 89 74 88 – 77 46 67 48 56 235 316 292 57 33 18 217 43 55 121 163 183
Endoplasmic Reticulum 2 1 1 0 0 0 – 0 1 0 0 0 0 2 1 4 3 1 0 0 4 1 1 1
Endosome 1 0 0 0 4 2 – 1 0 1 0 0 1 1 3 8 2 2 3 4 4 2 2 2
Golgi 0 0 0 0 0 0 – 1 0 0 1 0 1 1 0 0 1 0 0 2 0 3 3 2
Mitochondria 13 12 16 34 46 107 – 173 168 206 177 226 3 7 5 2 1 2 27 19 42 43 43 25
Nucleus 0 0 0 0 0 0 – 2 3 1 3 3 1 1 0 1 1 2 0 0 0 1 1 1
Nuclear Periphery 0 1 0 0 0 0 – 0 4 2 4 3 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 1 0 0 0 – 1 2 2 1 3 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 3 1 – 3 1 0 2 3 0 1 1 0 0 0 1 0 0 0 0 1
SpindlePole 0 0 1 0 1 6 – 17 4 9 11 19 1 3 1 0 0 2 3 1 1 3 5 5
Vac/Vac Membrane 0 0 0 0 3 8 – 6 4 5 3 5 4 4 5 5 1 1 5 2 5 9 13 14
Unique Cell Count 265 67 121 100 109 171 241 205 252 209 268 242 330 301 72 42 24 273 79 124 202 260 262
Labelled Cell Count 269 74 132 125 135 217 296 247 312 264 344 249 340 314 83 47 29 273 79 124 202 260 262


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.9 3.0 2.9 4.1 2.8 – 3.2 2.7 3.0 2.9 2.8 4.3 4.4 4.3 5.7 5.4 5.4 4.3 4.4 4.5
Std Deviation (1e-4) 0.8 0.8 0.7 1.2 2.3 0.8 – 1.8 1.1 1.4 1.5 0.9 1.0 1.2 1.2 1.7 1.4 1.5 1.6 1.5 1.5
Intensity Change (Log2) – – – -0.06 0.45 -0.1 – 0.1 -0.18 -0.02 -0.04 -0.14 0.5 0.53 0.52 0.92 0.83 0.84 0.5 0.55 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8852 -0.5208 -0.6351 -0.7142 -0.8126 -0.5214 -0.7103 -0.2948 -1.296 1.4813 0.1255 -0.4445 -0.1642 0.1177 0.1024 0.5339 0.091 0.1323
Actin 0.0133 0.0045 0.0225 0.0008 0.0107 0.0014 0.0218 0.0058 0.0007 0.0009 0.0052 0.0018 0.0121 0.0019 0.0003 0.0012 0.0004 0.0018
Bud 0.0007 0.0036 0.0008 0.0024 0.0026 0.0002 0.0016 0.019 0.0075 0.0036 0.0003 0.0017 0.0004 0.0004 0.0003 0.0018 0.0002 0.0001
Bud Neck 0.0018 0.0009 0.0008 0.0007 0.0021 0.0017 0.0014 0.0003 0.0099 0.0002 0.0004 0.0095 0.004 0.0003 0.0006 0.0004 0 0.001
Bud Periphery 0.0037 0.0049 0.0013 0.0058 0.01 0.0005 0.0053 0.0078 0.0041 0.0116 0.0005 0.0009 0.0009 0.0012 0.0009 0.0037 0.0007 0.0004
Bud Site 0.0013 0.0264 0.0019 0.0011 0.0014 0.0013 0.0123 0.0197 0.0122 0.0006 0.001 0.0006 0.0028 0.0042 0.0006 0.0016 0 0.0002
Cell Periphery 0.0008 0.0009 0.0003 0.0013 0.001 0.0003 0.001 0.0015 0.0008 0.0012 0.0001 0.0006 0.0009 0.001 0.0004 0.0011 0.0001 0.0002
Cytoplasm 0.073 0.1058 0.076 0.1034 0.0699 0.1432 0.0645 0.1091 0.0693 0.0119 0.0585 0.1426 0.0586 0.0959 0.0849 0.139 0.0791 0.1302
Cytoplasmic Foci 0.1032 0.0705 0.0518 0.0291 0.057 0.0532 0.0439 0.0188 0.0256 0.0277 0.098 0.0239 0.0521 0.0495 0.0194 0.0469 0.0101 0.0362
Eisosomes 0.0005 0.0004 0.0008 0.0005 0.0019 0.0002 0.001 0.0006 0.0003 0.0002 0.0003 0.0002 0.0006 0.0005 0.0003 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0029 0.0025 0.0023 0.0025 0.0032 0.0023 0.0043 0.0038 0.0018 0.0021 0.0017 0.0014 0.0045 0.0022 0.0037 0.0014 0.0013 0.0022
Endosome 0.06 0.0321 0.0323 0.0447 0.019 0.0242 0.044 0.016 0.011 0.0211 0.105 0.002 0.0297 0.0168 0.0122 0.0156 0.0018 0.012
Golgi 0.0074 0.0078 0.0073 0.0018 0.0067 0.0049 0.0114 0.0031 0.0023 0.0113 0.0562 0.0005 0.0071 0.0047 0.0021 0.0032 0.0002 0.0012
Lipid Particles 0.0255 0.0031 0.0115 0.0045 0.0402 0.0086 0.0114 0.0013 0.0028 0.0035 0.0394 0.0035 0.0143 0.0088 0.0035 0.0035 0.0002 0.0028
Mitochondria 0.0278 0.0076 0.0444 0.0978 0.0081 0.0525 0.1065 0.0533 0.0173 0.2343 0.1238 0.042 0.0129 0.0176 0.0054 0.0068 0.0004 0.0102
None 0.6432 0.7103 0.731 0.6758 0.7248 0.6851 0.5966 0.7165 0.8248 0.6332 0.4574 0.7533 0.7772 0.7808 0.852 0.7614 0.9035 0.7848
Nuclear Periphery 0.0022 0.0006 0.001 0.0011 0.0051 0.002 0.0139 0.001 0.0007 0.0013 0.0006 0.0004 0.003 0.0013 0.0012 0.0006 0.0003 0.0006
Nucleolus 0.0009 0.0009 0.0005 0.0012 0.0015 0.0006 0.0013 0.0012 0.0005 0.0004 0.0002 0.0008 0.0009 0.0005 0.0004 0.0003 0 0.0002
Nucleus 0.0012 0.0013 0.0009 0.0037 0.0012 0.0013 0.0047 0.0138 0.0013 0.0007 0.0003 0.0011 0.0012 0.0011 0.0008 0.0006 0.0002 0.0007
Peroxisomes 0.0114 0.0091 0.008 0.0042 0.021 0.0104 0.0085 0.0011 0.0012 0.0229 0.0445 0.0021 0.0077 0.0061 0.0073 0.0046 0.0006 0.0117
Punctate Nuclear 0.0059 0.0016 0.0012 0.0017 0.0083 0.0017 0.0372 0.0022 0.0025 0.0016 0.0012 0.0104 0.0028 0.0016 0.0005 0.0007 0.0001 0.0012
Vacuole 0.0113 0.0044 0.0025 0.0139 0.003 0.0032 0.0044 0.0033 0.003 0.0056 0.0031 0.0005 0.0055 0.0028 0.0027 0.0043 0.0004 0.0018
Vacuole Periphery 0.0017 0.0008 0.0009 0.002 0.0011 0.0013 0.003 0.0007 0.0008 0.004 0.0023 0.0003 0.001 0.0008 0.0005 0.001 0.0001 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.6229 4.1619 1.5442 11.9208 4.5273 23.2657 15.7804 24.8517 13.343 15.9651
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1372 903 298 1462 1978 1771 182 1429 3350 2674 480 2891

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 579.82 692.05 918.77 819.53 646.84 729.19 931.32 824.76 619.39 716.65 923.53 822.12
Standard Deviation 79.22 95.08 110.26 128.73 68.57 87.38 100.37 126.36 80.20 91.75 106.79 127.59
Intensity Change Log 2 — 0.255271 0.664099 0.499192 — 0.172886 0.525868 0.350565 — 0.212415 0.592860 0.422729

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000067 0.000436 0.002066 0.003519 0.000190 0.000392 0.000717 0.003036 0.000140 0.000407 0.001555 0.003280
Bud Neck 0.019884 0.051226 0.009946 0.032188 0.020474 0.057039 0.007533 0.036923 0.020233 0.055076 0.009031 0.034529
Bud Site 0.002274 0.019755 0.026742 0.057657 0.003602 0.013273 0.010543 0.063557 0.003058 0.015461 0.020600 0.060573
Cell Periphery 0.000256 0.000198 0.000271 0.000230 0.000104 0.000162 0.000198 0.000231 0.000166 0.000174 0.000243 0.000231
Cytoplasm 0.444785* 0.291595* 0.218231* 0.254638* 0.404217* 0.279067* 0.188746* 0.268477* 0.420832* 0.283298* 0.207051* 0.261479*
Cytoplasmic Foci 0.234172* 0.319356* 0.057886 0.108911 0.271049* 0.305591* 0.027525 0.119847 0.255946* 0.310239* 0.046374 0.114317
Eisosomes 0.000499 0.000219 0.000117 0.000132 0.000216 0.000200 0.000118 0.000116 0.000332 0.000206 0.000117 0.000124
Endoplasmic Reticulum 0.001188 0.000547 0.011186 0.004231 0.000858 0.000575 0.008567 0.003244 0.000993 0.000566 0.010193 0.003743
Endosome 0.014413 0.036434 0.016094 0.042808 0.012564 0.045059 0.007147 0.037908 0.013322 0.042146 0.012702 0.040386
Golgi 0.002942 0.013094 0.003064 0.010467 0.003600 0.010016 0.000328 0.013486 0.003331 0.011055 0.002027 0.011959
Lipid Particles 0.021393 0.012942 0.006831 0.006995 0.014960 0.014300 0.005641 0.005821 0.017595 0.013842 0.006379 0.006415
Mitochondria 0.005077 0.033284 0.001100 0.017579 0.010581 0.041722 0.001336 0.021563 0.008327 0.038873 0.001190 0.019548
Mitotic Spindle 0.000713 0.007537 0.010156 0.094944 0.000948 0.007831 0.011377 0.086779 0.000852 0.007732 0.010619 0.090908
None 0.014738 0.003102 0.002604 0.002943 0.002188 0.002685 0.002761 0.003173 0.007328 0.002826 0.002663 0.003057
Nuclear Periphery 0.000741 0.000399 0.002656 0.002773 0.000640 0.000569 0.003269 0.002035 0.000681 0.000511 0.002888 0.002408
Nuclear Periphery Foci 0.000560 0.000491 0.005183 0.002036 0.000169 0.000267 0.002908 0.001483 0.000329 0.000343 0.004320 0.001763
Nucleolus 0.003399 0.002345 0.001902 0.004273 0.001173 0.002610 0.002540 0.002878 0.002085 0.002521 0.002144 0.003583
Nucleus 0.184681* 0.100908 0.495097* 0.121601 0.195518* 0.101759 0.576991* 0.120095 0.191080* 0.101472 0.526149* 0.120857
Peroxisomes 0.006519 0.049257 0.014360 0.028112 0.009783 0.032709 0.005674 0.041465 0.008446 0.038297 0.011067 0.034712
Vacuole 0.040775 0.054440 0.113289 0.195679 0.046126 0.079576 0.135300 0.160843 0.043934 0.071088 0.121635 0.178460
Vacuole Periphery 0.000924 0.002436 0.001218 0.008282 0.001038 0.004599 0.000780 0.007039 0.000991 0.003868 0.001052 0.007668

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.67 -4.53 -5.27 -4.53 -1.18 -1.71 -4.59 -8.13 -7.15 -5.59 -3.46 -5.15 -8.37 -7.34 -2.68
Bud Neck -7.38 4.73 -3.34 5.28 -7.67 -12.22 4.98 -4.74 7.49 -7.85 -14.26 7.11 -5.73 9.60 -11.12
Bud Site -7.13 -4.01 -14.13 -6.93 -4.22 -7.94 -1.86 -14.37 -10.71 -9.98 -10.83 -4.28 -20.03 -13.08 -7.78
Cell Periphery 1.74 -0.49 2.26 0.11 3.47 -5.63 -5.50 -3.42 -0.85 1.07 -0.45 -4.65 -1.27 -0.91 3.31
Cytoplasm 13.62 17.60 19.63 3.57 -3.34 14.49 15.96 14.49 0.91 -7.05 20.15 22.91 23.74 3.14 -7.02
Cytoplasmic Foci -8.71 24.59 21.94 26.39 -6.80 -4.54 39.73 27.82 31.11 -14.20 -9.05 42.06 35.73 41.31 -12.61
Eisosomes 3.11 4.15 4.19 7.53 0.18 1.09 4.28 7.25 11.51 2.25 3.32 5.28 5.70 13.27 1.74
Endoplasmic Reticulum 3.11 -9.03 -8.32 -11.60 6.30 1.35 -8.26 -6.81 -8.87 6.17 2.85 -12.15 -10.77 -14.46 8.77
Endosome -7.27 -0.31 -7.97 0.79 -6.43 -14.56 3.91 -7.44 6.60 -9.93 -16.02 0.76 -11.10 5.50 -9.76
Golgi -6.52 0.66 -3.74 3.34 -3.31 -6.75 6.09 -3.13 1.21 -5.93 -9.55 2.55 -4.63 3.14 -5.81
Lipid Particles 4.50 8.95 9.55 5.51 0.22 0.54 8.62 9.65 9.21 0.47 3.61 12.16 13.56 10.80 0.69
Mitochondria -8.30 6.57 -4.84 5.67 -8.25 -10.64 7.57 -1.62 8.75 -7.50 -13.70 9.35 -3.62 10.80 -11.05
Mitotic Spindle -3.36 -3.06 -15.46 -13.41 -11.83 -5.05 -1.53 -14.52 -12.65 -10.68 -6.09 -3.37 -21.17 -18.50 -15.84
None 5.58 6.06 5.90 0.80 -0.21 -0.83 -0.48 -0.93 -0.26 -0.47 4.81 5.35 4.81 0.21 -0.53
Nuclear Periphery 2.09 -9.16 -7.66 -9.39 -1.34 0.86 -10.35 -9.21 -9.64 3.58 2.06 -14.53 -11.50 -12.90 1.16
Nuclear Periphery Foci -0.50 -5.18 -7.86 -2.87 3.16 -2.01 -4.22 -7.94 -7.13 2.60 -0.71 -6.73 -11.39 -6.84 4.19
Nucleolus 1.75 2.50 -0.66 -2.93 -3.78 -4.02 -0.97 -4.43 -1.40 -0.67 -1.17 0.08 -3.68 -2.96 -2.59
Nucleus 11.47 -16.50 6.41 -4.94 18.74 16.53 -15.81 6.47 -7.49 17.54 20.12 -22.49 9.36 -8.91 25.35
Peroxisomes -11.79 -1.32 -7.98 6.62 -2.91 -11.99 2.88 -7.81 1.15 -8.17 -17.02 0.20 -10.78 5.52 -6.57
Vacuole -3.99 -9.93 -30.28 -26.29 -9.68 -10.02 -8.26 -27.43 -21.00 -2.04 -10.87 -12.58 -40.70 -32.57 -8.13
Vacuole Periphery -6.11 -0.57 -5.53 -3.76 -5.34 -7.73 3.02 -4.17 0.26 -4.75 -9.04 0.81 -6.89 -1.74 -7.02
Standard name
Human Ortholog
Description Protein of unknown function; mitochondrial protein that physically interacts with Tim23p; null mutant displays reduced respiratory growth
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Aim19

Aim19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available