Standard name
Human Ortholog
Description Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; binds to SNARE complexes binteracting with Sec9p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.33 0.44 0.17 0.16 0.08 0.11 0.05 0.13 0.14 0.13 0.15 0.16 0.25 – – 0.24 0.24 0.19 0.33 0.31 0.35 0 0.1 0.09
Bud 0.14 0.23 0.15 0.22 0.22 0.22 0.15 0.17 0.2 0.19 0.19 0.16 0.19 – – 0.22 0.18 0.14 0.32 0.37 0.29 0.33 0.47 0.53
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0.1 0 0.2 0.17 0.2 0.27 0.25 0.12
Bud Site 0.23 0.21 0.15 0.18 0.11 0.11 0.08 0.07 0.06 0.11 0.09 0.09 0.19 – – 0.15 0.17 0.09 – – – – – –
Cell Periphery 0.06 0.07 0 0 0 0 0 0.06 0.05 0.06 0.08 0.08 0 – – 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.15 0.18 0.25 0.22 0.21 0.16 0.17 0.14 0.12 0.13 0.11 0.12 – – 0.28 0.25 0.29 0 0.05 0 0.1 0.06 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.12 – – 0 0 0.05 0 0 0 0 0 0
Endosome 0.06 0 0.11 0 0.16 0.07 0.09 0.06 0 0 0 0 0.38 – – 0 0 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.24 0.1 0.12 0.07 0.27 0.35 0.37 0.52 0.5 0.52 0.54 0.06 – – 0.07 0.1 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0.05 0.08 0 0 0.06 0.05 0 0.06 – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0.19 0.19 0.4 0.29 0.34 0.25 0.23 0.23 0.19 0.19 0.18 0.2 0.06 – – 0.15 0.14 0.22 0 0 0 0 0 0
SpindlePole 0.14 0 0.17 0.18 0.15 0.1 0.11 0.12 0 0.07 0.06 0.06 0.25 – – 0.21 0.26 0.26 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 53 41 41 34 21 32 24 64 48 42 47 58 4 – – 117 64 42 66 36 94 1 8 8
Bud 23 22 37 45 56 65 69 80 67 61 58 59 3 – – 105 48 30 65 43 77 13 38 51
Bud Neck 4 2 3 2 3 4 7 4 8 9 4 2 0 – – 7 26 6 41 19 53 11 19 11
Bud Site 38 20 36 37 28 33 37 35 20 35 29 33 3 – – 73 45 19 – – – – – –
Cell Periphery 10 7 10 9 6 13 20 31 16 19 26 31 0 – – 22 7 8 2 1 5 0 0 0
Cytoplasm 21 14 44 52 55 63 73 82 47 39 39 42 2 – – 133 68 63 2 5 7 4 4 11
Endoplasmic Reticulum 2 3 5 3 2 6 5 11 10 12 9 9 2 – – 14 10 12 0 0 1 1 0 0
Endosome 9 4 26 7 40 20 39 27 11 8 5 8 6 – – 14 11 17 6 2 4 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 5 0 3 1 0 1 0 0 0
Mitochondria 15 23 24 25 18 82 159 175 175 163 162 197 1 – – 34 27 10 3 1 3 1 0 2
Nucleus 7 1 7 7 9 5 14 13 3 5 9 4 0 – – 25 7 9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 2 0 2 1 0 0 0 – – 0 0 0 0 0 0 1 0 0
Nucleolus 11 2 9 4 10 15 36 20 15 19 14 6 1 – – 10 3 3 0 0 0 0 0 0
Peroxisomes 31 18 95 61 87 75 105 108 64 64 56 75 1 – – 73 38 49 3 1 9 0 0 0
SpindlePole 23 3 40 37 39 31 51 57 12 23 17 21 4 – – 101 71 57 6 3 6 1 1 2
Vac/Vac Membrane 1 0 4 1 5 3 12 10 1 4 1 6 0 – – 9 2 1 1 0 2 0 1 1
Unique Cell Count 162 94 240 207 253 300 451 477 336 329 309 367 16 483 271 221 203 118 270 41 81 98
Labelled Cell Count 248 160 381 324 380 447 653 717 499 504 476 551 27 742 427 329 203 118 270 41 81 98


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.9 6.2 5.9 6.0 5.3 5.0 5.5 5.1 4.9 4.9 5.1 6.0 – – 6.6 6.7 7.9 – 6.9 7.8
Std Deviation (1e-4) 1.4 1.4 1.4 1.6 2.0 1.6 1.5 1.4 1.2 1.2 1.3 1.3 0.6 – – 1.3 1.3 1.8 – 1.4 2.0
Intensity Change (Log2) – – – -0.06 -0.04 -0.23 -0.29 -0.17 -0.28 -0.33 -0.32 -0.28 -0.03 – – 0.09 0.12 0.36 – 0.17 0.34

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU8002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -2.9 -2.2 -5.0 -1.3 -0.9 -1.4 -0.6 -0.4 0 – – 2.2 1.8 0.5
Bud 1.7 1.9 1.8 0 0.5 1.4 1.0 1.0 0.2 0 – – 2.0 0.7 -0.6
Bud Neck 0 0 0 0 0 0 1.2 0 0 0 – – 0 4.1 0
Bud Site 0.8 -1.3 -1.4 -2.8 -3.2 -3.6 -1.6 -2.0 -2.3 0 – – 0 0.5 -2.1
Cell Periphery 0.1 -1.1 0.1 0.2 1.3 0.3 0.9 2.0 2.1 0 – – 0.2 -1.0 -0.3
Cytoplasm 1.7 0.9 0.8 -0.7 -0.4 -1.4 -2.2 -1.9 -2.4 0 – – 2.7 1.8 2.6
Endoplasmic Reticulum 0 0 0 0 0.2 0.7 1.1 0.6 0.3 0 – – 0.6 1.1 1.9
Endosome -3.0 1.6 -1.7 -0.9 -2.5 -3.6 -4.2 -4.6 -4.5 0 – – -4.4 -2.9 -1.2
Golgi 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Mitochondria 0.7 -1.1 5.0 7.2 7.5 10.5 9.9 10.4 10.9 0 – – -1.4 0 -2.2
Nucleus 0.3 0.4 -1.0 0.1 -0.1 0 -1.1 0 0 0 – – 1.4 -0.2 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Nucleolus -1.1 0.1 0.7 2.1 0.3 0.4 1.1 0.5 -1.6 0 – – -1.3 -2.0 -1.6
Peroxisomes -2.2 -1.2 -3.6 -4.5 -4.8 -5.4 -5.3 -5.6 -5.1 -2.7 – – -7.3 -6.6 -4.0
SpindlePole 0.3 -0.4 -2.2 -2.0 -1.7 -5.4 -3.6 -4.3 -4.4 0 – – 1.4 2.6 2.4
Vacuole 0 0 0 0.8 0 0 0 0 0 0 – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7609 2.3374 2.7062 2.9589 2.8904 2.7987 5.0162 3.9045 4.1821 4.5074 4.4364 4.7228 4.4501 3.8151 4.0244 3.7171 4.0954 4.706
Actin 0.2299 0.0964 0.139 0.3221 0.3079 0.1673 0.2152 0.08 0.1304 0.2868 0.4327 0.1423 0.2469 0.1132 0.1405 0.316 0.3278 0.146
Bud 0.0749 0.0907 0.2722 0.124 0.0901 0.0099 0.0263 0.078 0.2293 0.0924 0.0551 0.0089 0.0507 0.0995 0.251 0.1006 0.0632 0.0097
Bud Neck 0.0964 0.0091 0.0108 0.0027 0.065 0.6389 0.1084 0.0162 0.0301 0.0327 0.0538 0.6822 0.0527 0.0067 0.0102 0.0124 0.0249 0.6936
Bud Periphery 0.1074 0.0756 0.394 0.4846 0.3552 0.0237 0.0479 0.0663 0.4152 0.3343 0.2649 0.0309 0.1063 0.0807 0.4076 0.4008 0.3452 0.0264
Bud Site 0.2582 0.5227 0.0767 0.0512 0.0652 0.0305 0.3548 0.6341 0.1101 0.1263 0.0419 0.0237 0.3731 0.5115 0.1453 0.1007 0.0912 0.0216
Cell Periphery 0.0119 0.0081 0.0037 0.0026 0.0034 0.0035 0.0157 0.0041 0.0042 0.0182 0.0052 0.0055 0.0317 0.0083 0.0066 0.0041 0.0073 0.0067
Cytoplasm 0.0714 0.1056 0.0259 0.0029 0.0191 0.0329 0.0442 0.0609 0.019 0.0233 0.0184 0.0102 0.0526 0.0831 0.0113 0.0244 0.0191 0.0258
Cytoplasmic Foci 0.0589 0.0274 0.0223 0.002 0.0396 0.0483 0.0648 0.0235 0.009 0.0146 0.028 0.0487 0.0323 0.0136 0.0046 0.0121 0.0236 0.0347
Eisosomes 0.0021 0.0006 0.0007 0.0006 0.0019 0.0005 0.0059 0.0003 0.0004 0.0016 0.001 0.0007 0.003 0.0008 0.0006 0.0003 0.0014 0.0005
Endoplasmic Reticulum 0.0039 0.0024 0.0013 0.0003 0.0005 0.0005 0.0035 0.0007 0.0028 0.0014 0.0024 0.0032 0.0014 0.0006 0.0005 0.0009 0.0004 0.0002
Endosome 0.0107 0.002 0.0039 0.0004 0.0013 0.0033 0.0161 0.0035 0.0026 0.0007 0.0202 0.002 0.0017 0.0022 0.0004 0.0019 0.0009 0.0013
Golgi 0.0092 0.0026 0.0051 0.0011 0.0027 0.0041 0.0123 0.0017 0.0008 0.0019 0.0089 0.003 0.0024 0.0011 0.0008 0.0035 0.0027 0.0025
Lipid Particles 0.0067 0.0012 0.0033 0.0001 0.003 0.0019 0.0124 0.001 0.0008 0.0082 0.0024 0.0016 0.0028 0.0009 0.0004 0.0004 0.0041 0.0009
Mitochondria 0.0132 0.001 0.0039 0.0021 0.0053 0.0006 0.0066 0.0036 0.0013 0.0018 0.0036 0.0028 0.0039 0.0012 0.002 0.0022 0.004 0.001
None 0.0197 0.0404 0.0171 0.0012 0.0233 0.0103 0.0152 0.0126 0.0236 0.0166 0.0148 0.0091 0.0118 0.0568 0.0078 0.0042 0.0339 0.0082
Nuclear Periphery 0.002 0.0006 0.0005 0.0002 0.0003 0.0001 0.0105 0.0004 0.0014 0.0004 0.0017 0.0024 0.0005 0.0004 0.0002 0.0005 0.0003 0
Nucleolus 0.0012 0.0021 0.0003 0.0001 0.0009 0.0013 0.0015 0.0013 0.0013 0.0003 0.0008 0.0007 0.0008 0.0042 0.0015 0.0005 0.0023 0.0003
Nucleus 0.001 0.0012 0.0007 0.0002 0.0006 0.0005 0.0069 0.0016 0.0009 0.0008 0.0017 0.0014 0.001 0.0034 0.001 0.0012 0.0012 0.0003
Peroxisomes 0.015 0.0033 0.0151 0.0008 0.0093 0.0085 0.0174 0.0043 0.0067 0.0327 0.03 0.0161 0.0179 0.0037 0.0048 0.0062 0.0428 0.0163
Punctate Nuclear 0.0024 0.0051 0.002 0.0003 0.004 0.012 0.0088 0.0033 0.009 0.0011 0.0053 0.0031 0.0014 0.0051 0.0013 0.0008 0.0023 0.0033
Vacuole 0.0034 0.0017 0.0013 0.0006 0.0012 0.0014 0.0045 0.0022 0.0009 0.0037 0.0059 0.001 0.0046 0.0027 0.0014 0.0058 0.0012 0.0006
Vacuole Periphery 0.0004 0.0002 0.0003 0.0002 0.0002 0.0001 0.0013 0.0004 0.0001 0.0003 0.0013 0.0003 0.0004 0.0002 0.0002 0.0004 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.8307 11.83 18.7675 20.7651 15.6788 13.2262 21.5509 22.6881 22.1602 20.5489
Translational Efficiency 0.6471 1.0428 0.6366 0.6668 0.6673 0.8338 0.6804 0.525 0.5598 0.6495

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
267 1774 347 1502 1632 1790 640 2061 1899 3564 987 3563

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 951.01 836.82 956.05 941.78 778.12 835.49 948.89 968.04 802.43 836.15 951.41 956.97
Standard Deviation 126.13 120.59 100.17 133.47 100.82 239.09 131.24 126.66 120.77 189.60 121.28 130.23
Intensity Change Log 2 — -0.184543 0.007626 -0.014070 — 0.102630 0.286248 0.315074 — -0.048204 0.139699 0.143390

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.179377 0.123152 0.074193 0.155338 0.158533 0.082988 0.148348 0.142099 0.161464 0.102980 0.122277 0.147680
Bud Neck 0.048616 0.118050 0.144468 0.059753 0.163744 0.190293 0.097519 0.072887 0.147557 0.154334 0.114025 0.067350
Bud Site 0.561916 0.519044 0.351051 0.601664 0.450113 0.517772 0.638820 0.631245 0.465833 0.518405 0.537649 0.618775
Cell Periphery 0.017266 0.013225 0.005586 0.016563 0.038292 0.027183 0.004532 0.019845 0.035335 0.020235 0.004903 0.018461
Cytoplasm 0.060215 0.092584 0.095089 0.090351 0.073720 0.078163 0.044601 0.098235 0.071821 0.085341 0.062351 0.094912
Cytoplasmic Foci 0.020986 0.042686 0.100276 0.007753 0.032016 0.026791 0.015352 0.002811 0.030465 0.034703 0.045209 0.004894
Eisosomes 0.008949 0.002217 0.003166 0.005810 0.008165 0.002574 0.001366 0.005179 0.008275 0.002397 0.001999 0.005445
Endoplasmic Reticulum 0.003709 0.000460 0.003003 0.001240 0.000431 0.000593 0.001201 0.000556 0.000892 0.000527 0.001834 0.000844
Endosome 0.006843 0.012933 0.025496 0.003713 0.006909 0.008560 0.002919 0.000875 0.006899 0.010737 0.010856 0.002071
Golgi 0.009557 0.014562 0.006203 0.002757 0.007664 0.006977 0.001349 0.001278 0.007930 0.010752 0.003056 0.001902
Lipid Particles 0.001228 0.001670 0.023896 0.000499 0.001930 0.001306 0.000791 0.000225 0.001831 0.001487 0.008914 0.000341
Mitochondria 0.003603 0.008441 0.000833 0.004295 0.011601 0.017758 0.000714 0.001500 0.010476 0.013120 0.000756 0.002678
Mitotic Spindle 0.004635 0.002547 0.002276 0.010312 0.000708 0.001625 0.001216 0.002763 0.001260 0.002084 0.001588 0.005946
None 0.000040 0.000703 0.000140 0.000150 0.000298 0.001190 0.000062 0.000094 0.000261 0.000947 0.000089 0.000118
Nuclear Periphery 0.000187 0.000011 0.000138 0.000073 0.000013 0.000010 0.000019 0.000009 0.000037 0.000011 0.000061 0.000036
Nuclear Periphery Foci 0.000576 0.000493 0.001490 0.000379 0.000088 0.000132 0.000217 0.000058 0.000157 0.000311 0.000664 0.000193
Nucleolus 0.000028 0.000163 0.000332 0.000035 0.000040 0.000200 0.000047 0.000013 0.000039 0.000182 0.000147 0.000022
Nucleus 0.001222 0.001396 0.027876 0.003811 0.001461 0.003334 0.003582 0.002822 0.001427 0.002369 0.012123 0.003239
Peroxisomes 0.020087 0.037924 0.070684 0.010259 0.039368 0.027030 0.016965 0.004299 0.036657 0.032453 0.035851 0.006811
Vacuole 0.048126 0.007217 0.063459 0.023436 0.004649 0.004802 0.020317 0.013040 0.010762 0.006004 0.035485 0.017422
Vacuole Periphery 0.002834 0.000524 0.000342 0.001810 0.000260 0.000718 0.000065 0.000167 0.000622 0.000621 0.000162 0.000860

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.57 5.05 1.17 -2.74 -6.93 8.99 1.19 2.06 -7.39 0.25 7.65 4.16 2.13 -6.75 -2.79
Bud Neck -7.93 -6.27 -1.26 10.32 6.26 -3.01 7.55 13.05 16.87 2.94 -1.03 4.45 13.52 19.13 6.57
Bud Site 1.09 6.90 -1.43 -5.04 -11.57 -5.81 -10.89 -15.13 -9.77 0.02 -5.51 -4.25 -14.11 -10.74 -6.27
Cell Periphery 0.87 3.04 0.18 -1.19 -5.03 3.47 12.29 6.08 3.24 -8.33 5.81 12.58 6.65 1.50 -9.74
Cytoplasm -3.02 -2.62 -3.90 -1.31 -0.21 -0.85 3.65 -5.88 -5.12 -8.78 -2.92 0.76 -6.86 -4.44 -6.46
Cytoplasmic Foci -4.27 -7.82 3.19 12.56 9.94 1.73 4.63 12.50 11.58 5.62 -1.56 -3.64 11.91 17.44 10.62
Eisosomes 2.42 1.98 1.10 -3.50 -1.89 5.47 6.61 2.73 -3.59 -5.25 6.19 6.30 2.87 -5.06 -5.06
Endoplasmic Reticulum 2.03 0.31 1.46 -2.79 2.20 -0.59 -2.94 -1.19 -0.07 2.39 1.29 -2.70 -0.11 -1.99 2.99
Endosome -3.20 -4.43 1.85 7.13 5.53 -1.48 3.23 7.84 8.98 2.08 -3.92 -2.30 6.63 11.36 5.61
Golgi -1.39 0.79 1.72 8.04 2.88 0.65 6.81 6.99 7.02 0.07 -2.50 4.95 6.47 10.58 1.88
Lipid Particles -0.56 -4.10 1.71 2.66 4.25 1.04 2.05 3.72 2.69 2.81 0.74 -3.56 3.71 3.87 4.38
Mitochondria -2.63 1.59 -0.36 3.18 -3.48 -2.87 8.49 7.86 9.30 -2.36 -1.78 8.65 6.62 9.69 -4.14
Mitotic Spindle 1.19 1.35 -2.30 -4.04 -4.17 -2.88 -2.44 -3.21 -1.67 -2.26 -2.00 -1.04 -5.30 -4.21 -4.86
None -3.66 -3.84 -4.56 2.98 -0.44 -2.56 4.27 3.62 3.14 -2.84 -3.43 3.53 2.84 4.18 -2.03
Nuclear Periphery 1.34 0.24 0.77 -3.10 2.03 0.47 -2.89 -0.09 -1.15 3.49 1.39 -1.84 -0.29 -3.22 2.36
Nuclear Periphery Foci 0.32 -2.18 0.75 0.35 2.93 -1.63 -3.81 1.50 2.46 4.21 -1.14 -4.11 -0.55 0.77 3.61
Nucleolus -2.48 -4.36 -0.61 2.36 4.28 -2.50 -0.67 3.28 2.92 3.17 -3.40 -4.17 1.98 3.80 4.85
Nucleus 0.37 -5.80 -4.37 -4.54 4.95 -1.86 -3.55 -4.83 -0.25 1.78 -1.66 -6.65 -6.20 -3.01 5.07
Peroxisomes -3.28 -5.69 2.20 10.09 8.12 3.49 6.19 12.09 10.05 5.06 1.45 0.39 11.51 14.61 9.09
Vacuole 5.26 -0.44 2.11 -9.40 3.51 -0.07 -6.24 -7.57 -7.72 3.29 4.07 -7.58 -5.89 -11.87 4.35
Vacuole Periphery 1.67 1.81 0.69 -2.25 -2.61 -2.62 5.48 1.45 3.06 -1.72 -0.09 2.35 -0.75 -0.82 -2.91
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; binds to SNARE complexes binteracting with Sec9p
Localization
Cell Percentages actin (34%), bud neck (12%), mixed (20%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec6

Sec6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available