Standard name
Human Ortholog
Description Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; has mRNA binding activity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.46 0.44 0.4 0.31 0.63 0.24 – 0.24 0.28 0.29 0.32 0.26 0.53 0.54 0.54 0.3 – 0.71 0.8 0.84 0.85 0.81 0.79 0.8
Bud 0.16 0 0.12 0.18 0.2 0.05 – 0.11 0.17 0.22 0.1 0.13 0.05 0.13 0.08 0 – 0 0 0 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0.08 0.07 0.06 0.05 0 – 0 0 0 0 0 0.08 0.07 0.12 0 – 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0 0 0 0.06 – 0.11 0.1 0.05 0.05 0 0.07 0.06 0 0.12 – 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0.1 0.15 0 0 0 0 – 0 0 0 0 0 0.05 0.11 0.1 0 – 0.18 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0.14 0.28 0 0 0.17 0.07 – 0.09 0.05 0.07 0 0 0.35 0.22 0.39 0.39 – 0.71 0.11 0 0 0 0 0.07
Mitochondria 0.7 0.41 0.9 0.79 0.71 0.73 – 0.68 0.72 0.71 0.8 0.85 0.56 0.54 0.35 0.12 – 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0.06 0 0.06 0.06 0 0.09 – 0.09 0.05 0.08 0.09 0 0 0 0 0.09 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.07 0.08 – 0.1 0.06 0.08 0.09 0.11 0 0.05 0.09 0 – 0 0 0 0 0 0.05 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.15 – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 23 17 29 32 26 27 – 55 46 53 54 67 54 54 53 10 – 12 74 67 92 68 81 100
Bud 8 0 9 18 8 6 – 25 29 39 17 34 5 13 8 1 – 0 2 1 2 4 1 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 4 2 0 2 – 5 4 5 4 8 2 0 0 0 – 0 – – – – – –
Cell Periphery 0 3 5 6 2 1 – 2 2 2 1 6 8 7 12 1 – 0 0 0 0 0 0 0
Cytoplasm 3 1 1 3 1 7 – 25 17 9 9 6 7 6 4 4 – 1 1 0 0 0 1 0
Endoplasmic Reticulum 5 6 3 4 1 1 – 3 2 2 4 2 5 11 10 1 – 3 0 0 0 1 0 0
Endosome 0 0 0 3 0 0 – 0 0 5 0 0 0 1 1 0 – 0 1 0 2 1 2 1
Golgi 7 11 1 4 7 8 – 20 9 12 4 6 35 22 39 13 – 12 9 3 2 1 4 8
Mitochondria 35 16 65 81 29 82 – 156 120 127 135 214 57 54 35 4 – 0 0 3 2 0 3 3
Nucleus 0 0 0 0 0 1 – 4 1 1 0 1 1 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 1 – 1 2 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 3 1 4 6 1 10 – 20 8 14 15 11 0 0 0 3 – 0 0 0 0 0 0 1
Peroxisomes 0 0 2 4 3 9 – 24 10 15 16 29 0 5 9 1 – 0 0 0 2 2 5 3
SpindlePole 0 0 0 1 0 0 – 6 4 5 1 3 0 1 1 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 2 1 0 2 – 2 2 0 1 1 0 0 0 5 – 0 0 0 0 0 0 0
Unique Cell Count 50 39 72 102 41 112 229 166 180 169 253 101 100 99 33 17 93 80 108 84 103 125
Labelled Cell Count 85 55 125 166 78 157 348 256 289 262 388 174 174 172 43 28 93 80 108 84 103 125


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 27.1 38.0 19.8 24.7 26.4 22.3 – 21.0 20.3 21.2 19.1 20.1 28.5 27.4 30.3 35.9 – 38.2 28.2 31.3 30.1
Std Deviation (1e-4) 9.4 12.2 6.8 8.9 9.3 8.3 – 9.5 8.7 8.0 7.6 7.5 8.0 9.5 10.4 19.3 – 15.0 12.2 12.0 12.2
Intensity Change (Log2) – – – 0.32 0.42 0.17 – 0.08 0.03 0.1 -0.05 0.02 0.53 0.47 0.61 0.86 – 0.95 0.51 0.66 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.2 2.4 -2.3 – -2.7 -1.9 -1.7 -1.2 -2.3 1.7 1.8 1.7 -1.0 – 2.3
Bud 0.9 1.0 -1.7 – -0.4 1.0 1.7 -0.6 0.2 -1.8 0.1 -1.0 0 – 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0.2 0 1.1 0 – 0
Cytoplasm 0 0 0 – 2.5 2.4 0 0 0 0 0 0 0 – 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 1.6 1.4 0 – 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 – 2.1 0 0 0 0 5.3 3.9 5.8 0 – 0
Mitochondria -1.9 -2.7 -2.8 – -3.7 -3.1 -3.3 -2.0 -1.2 -4.8 -5.1 -7.2 -7.8 – -7.5
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0.8 – 0.9 0 0.6 0.9 0 0 0 0 0 – 0
Peroxisomes 0 0 0 – 2.0 0 0 1.8 2.2 0 0 0 0 – 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.4694 22.8342 21.7384 21.5739 20.7676 22.3648 18.3606 21.301 20.5494 20.0677 21.9628 21.7546 22.658 24.6112 24.2522 27.6948 21.6515 27.0781
Actin 0.8653 0.8993 0.8674 0.9645 0.8895 0.8142 0.782 0.9156 0.8872 0.9106 0.8572 0.9499 0.7247 0.9121 0.9086 0.9794 0.8501 0.9442
Bud 0.0003 0.0005 0.0007 0.0003 0.0006 0.0007 0.0009 0.0005 0.0012 0.0003 0.0012 0 0.0011 0.0003 0.0004 0.0001 0.0005 0.0002
Bud Neck 0.0032 0.0028 0.0063 0.0042 0.0104 0.0076 0.0024 0.0023 0.007 0.0014 0.0042 0.0023 0.0009 0.0041 0.0023 0.0047 0.0096 0.0045
Bud Periphery 0.0004 0.0004 0.0008 0.0004 0.0013 0.0019 0.0015 0.0005 0.0023 0.0008 0.0029 0 0.0018 0.0002 0.0005 0.0001 0.0009 0.0004
Bud Site 0.0019 0.0559 0.0146 0.0012 0.0011 0.0018 0.0077 0.0343 0.0132 0.0007 0.0041 0.0001 0.0065 0.0247 0.0061 0.0002 0.001 0.0004
Cell Periphery 0.0003 0.0004 0.0005 0.0001 0.0006 0.0025 0.0006 0.0008 0.0009 0.0002 0.0019 0.0001 0.0003 0.0004 0.0002 0.0001 0.0002 0.0002
Cytoplasm 0.0073 0 0.0047 0.0006 0.0016 0.0001 0.0027 0.0001 0.0057 0 0.0001 0 0.0027 0 0.0001 0 0.0006 0.0001
Cytoplasmic Foci 0.0103 0.0006 0.0043 0.0067 0.0022 0.0019 0.0192 0.0032 0.0016 0.0003 0.0125 0 0.0327 0.0002 0.0029 0 0.0036 0.0003
Eisosomes 0.0333 0.0345 0.0509 0.0037 0.0077 0.0877 0.0773 0.0332 0.0371 0.011 0.0612 0.0466 0.0374 0.0554 0.0377 0.0149 0.0024 0.0426
Endoplasmic Reticulum 0.0044 0 0.0002 0.0007 0.0088 0.0002 0.0023 0 0.0015 0.0007 0 0 0.0083 0 0.0002 0 0.0039 0.0002
Endosome 0.0078 0 0.0023 0.0016 0.0366 0.0043 0.0164 0.0002 0.0123 0.0025 0.0003 0 0.0201 0 0.0026 0 0.0371 0.0011
Golgi 0.0056 0.0016 0.0016 0.0035 0.0136 0.0373 0.0144 0.0014 0.0111 0.0071 0.0031 0.0004 0.0171 0.0002 0.005 0.0003 0.0286 0.0029
Lipid Particles 0.0129 0.0001 0.0005 0.0032 0.0039 0.0088 0.0165 0.0005 0.0006 0.0011 0.0143 0 0.0374 0.0001 0.0203 0.0001 0.0146 0.0004
Mitochondria 0.0189 0.0014 0.0328 0.003 0.0055 0.0263 0.0191 0.0052 0.0153 0.0602 0.0107 0.0001 0.0505 0.0012 0.0064 0 0.0286 0.0015
None 0.0037 0 0.0019 0.0019 0.0012 0.0001 0.0029 0.0001 0.0001 0.0003 0.0001 0 0.0063 0 0.0003 0 0.0014 0.0001
Nuclear Periphery 0.0151 0 0.0007 0.0003 0.0103 0.0005 0.011 0 0.0004 0.001 0 0 0.0133 0 0.0004 0 0.0085 0.0003
Nucleolus 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0006 0.0001 0 0.0001 0.0001 0 0.0013 0 0 0 0.0002 0
Nucleus 0.001 0 0.0014 0.0001 0.0007 0.0001 0.0012 0 0.0002 0.0002 0 0 0.0015 0 0 0 0.0006 0
Peroxisomes 0.0032 0.0023 0.003 0.0036 0.0011 0.0025 0.0125 0.0019 0.0006 0.0006 0.0258 0.0003 0.0238 0.0009 0.0054 0.0001 0.0023 0.0005
Punctate Nuclear 0.0029 0 0.0051 0.0004 0.0009 0.0004 0.0067 0 0.0001 0.0001 0.0001 0 0.0063 0 0.0002 0 0.0017 0.0001
Vacuole 0.0005 0 0.0002 0.0001 0.0011 0.0004 0.0012 0.0001 0.0012 0.0002 0.0001 0 0.0023 0 0.0001 0 0.0011 0
Vacuole Periphery 0.0014 0 0.0001 0.0001 0.0012 0.0006 0.0008 0 0.0004 0.0008 0.0001 0 0.0037 0 0.0003 0 0.0027 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 75.9332 86.9708 77.1285 105.4201 103.0656 92.4924 91.1533 114.3591 123.1672 102.0601
Translational Efficiency 2.7413 2.4339 2.8807 2.0527 2.1607 2.9759 2.6189 2.1108 2.2904 2.208

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1177 697 813 1321 1743 10 1783 1122 2920 707 2596 2443

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1852.12 1958.62 2652.44 2535.62 1768.30 1717.60 2483.14 2464.72 1802.09 1955.21 2536.16 2503.06
Standard Deviation 520.69 538.17 552.11 659.52 458.27 496.07 676.13 630.24 486.14 538.35 644.68 647.20
Intensity Change Log 2 — 0.080660 0.518143 0.453161 — -0.041969 0.489803 0.479061 — 0.022066 0.504370 0.465869

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.866317* 0.815839* 0.863941* 0.802346* 0.872788* 0.734197* 0.850760* 0.732383* 0.870180* 0.814684* 0.854888* 0.770214*
Bud Neck 0.007157 0.005008 0.015961 0.007700 0.004239 0.002096 0.007930 0.009980 0.005415 0.004967 0.010445 0.008747
Bud Site 0.014502 0.009465 0.067062 0.026112 0.004590 0.000767 0.040367 0.028795 0.008585 0.009342 0.048727 0.027344
Cell Periphery 0.001482 0.000739 0.002123 0.005883 0.000514 0.000247 0.001866 0.005525 0.000904 0.000732 0.001946 0.005719
Cytoplasm 0.000904 0.000958 0.001012 0.000799 0.000146 0.000116 0.001893 0.000306 0.000451 0.000946 0.001617 0.000572
Cytoplasmic Foci 0.003560 0.001933 0.005311 0.002548 0.000425 0.006158 0.004125 0.002392 0.001689 0.001992 0.004497 0.002476
Eisosomes 0.068356 0.092675 0.011304 0.121823* 0.094826 0.025345 0.039202 0.178529* 0.084156 0.091722 0.030465 0.147866*
Endoplasmic Reticulum 0.002433 0.007910 0.002175 0.004250 0.001604 0.001830 0.003711 0.007883 0.001938 0.007824 0.003230 0.005919
Endosome 0.002087 0.005126 0.004765 0.003699 0.001275 0.032151 0.005964 0.004741 0.001603 0.005508 0.005589 0.004177
Golgi 0.005049 0.018016 0.004210 0.005411 0.005293 0.159444* 0.006330 0.006596 0.005195 0.020016 0.005666 0.005955
Lipid Particles 0.006550 0.022098 0.009907 0.003323 0.005335 0.034230 0.019043 0.009206 0.005825 0.022270 0.016182 0.006025
Mitochondria 0.013628 0.011988 0.006169 0.008827 0.006505 0.000400 0.009349 0.008887 0.009376 0.011824 0.008353 0.008854
Mitotic Spindle 0.000246 0.001537 0.000672 0.001330 0.000582 0.000020 0.000985 0.001406 0.000447 0.001515 0.000887 0.001365
None 0.000170 0.000336 0.000050 0.000126 0.000149 0.000036 0.000071 0.000084 0.000158 0.000332 0.000064 0.000107
Nuclear Periphery 0.000040 0.000099 0.000074 0.000089 0.000020 0.000023 0.000106 0.000077 0.000028 0.000098 0.000096 0.000084
Nuclear Periphery Foci 0.000181 0.000407 0.000058 0.000125 0.000011 0.000010 0.000200 0.000061 0.000080 0.000401 0.000156 0.000096
Nucleolus 0.000018 0.000053 0.000017 0.000027 0.000010 0.000003 0.000087 0.000020 0.000013 0.000052 0.000065 0.000024
Nucleus 0.000058 0.000387 0.000090 0.000076 0.000045 0.000023 0.000093 0.000064 0.000051 0.000382 0.000092 0.000071
Peroxisomes 0.006822 0.003961 0.001989 0.003342 0.001411 0.002192 0.005778 0.000846 0.003592 0.003936 0.004591 0.002196
Vacuole 0.000244 0.000738 0.002757 0.001409 0.000130 0.000191 0.001568 0.001413 0.000176 0.000731 0.001940 0.001411
Vacuole Periphery 0.000195 0.000728 0.000354 0.000755 0.000101 0.000521 0.000571 0.000807 0.000139 0.000725 0.000503 0.000779

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.25 0.74 6.31 1.07 4.84 NaN NaN NaN NaN NaN 5.18 2.92 13.52 3.76 10.27
Bud Neck 3.04 -6.03 -0.80 -3.74 5.49 NaN NaN NaN NaN NaN 0.85 -7.74 -5.80 -6.11 2.33
Bud Site 1.76 -8.81 -3.43 -4.93 6.67 NaN NaN NaN NaN NaN -0.35 -13.98 -8.14 -6.22 6.47
Cell Periphery 1.89 -1.25 -4.26 -5.28 -3.50 NaN NaN NaN NaN NaN 0.81 -3.61 -7.61 -7.95 -5.67
Cytoplasm -0.20 -0.93 0.32 0.36 1.13 NaN NaN NaN NaN NaN -0.82 -3.54 -0.73 0.67 3.33
Cytoplasmic Foci 1.63 -1.46 0.78 -0.90 2.03 NaN NaN NaN NaN NaN -0.51 -4.04 -1.34 -0.87 2.82
Eisosomes -2.82 12.29 -6.69 -2.96 -16.52 NaN NaN NaN NaN NaN -0.95 13.85 -10.23 -6.26 -20.45
Endoplasmic Reticulum -2.59 0.43 -2.31 1.75 -2.98 NaN NaN NaN NaN NaN -2.90 -2.63 -5.90 0.93 -3.66
Endosome -2.44 -3.63 -2.21 1.05 1.19 NaN NaN NaN NaN NaN -3.19 -6.85 -4.84 1.02 1.96
Golgi -4.67 0.83 -0.30 4.44 -1.03 NaN NaN NaN NaN NaN -4.90 -0.61 -0.84 4.58 -0.30
Lipid Particles -4.01 -1.50 2.14 5.05 3.23 NaN NaN NaN NaN NaN -4.46 -5.67 -0.17 4.34 5.28
Mitochondria 0.51 3.29 2.09 1.10 -1.54 NaN NaN NaN NaN NaN -0.92 0.79 0.36 1.08 -0.37
Mitotic Spindle -1.19 -1.48 -1.99 0.07 -1.15 NaN NaN NaN NaN NaN -0.96 -1.24 -1.65 0.08 -0.95
None -1.09 6.05 1.41 1.34 -3.23 NaN NaN NaN NaN NaN -1.16 8.19 2.93 1.46 -3.08
Nuclear Periphery -2.04 -2.42 -2.00 0.20 -0.66 NaN NaN NaN NaN NaN -2.63 -2.93 -3.98 0.44 0.45
Nuclear Periphery Foci -0.87 0.88 0.39 1.25 -1.02 NaN NaN NaN NaN NaN -1.45 -0.82 -0.21 1.42 0.76
Nucleolus -1.03 0.01 -1.57 0.68 -1.52 NaN NaN NaN NaN NaN -1.15 -1.93 -2.46 0.80 1.47
Nucleus -1.31 -1.89 -1.40 1.21 1.02 NaN NaN NaN NaN NaN -1.34 -2.70 -2.06 1.24 1.49
Peroxisomes 1.42 3.66 2.20 0.33 -1.27 NaN NaN NaN NaN NaN -0.21 -1.15 1.85 1.04 2.68
Vacuole -1.47 -4.41 -2.55 -1.19 2.14 NaN NaN NaN NaN NaN -1.70 -6.03 -4.05 -1.51 1.52
Vacuole Periphery -2.18 -1.67 -2.23 -0.07 -1.52 NaN NaN NaN NaN NaN -2.47 -3.25 -3.69 -0.17 -1.31
Standard name
Human Ortholog
Description Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; has mRNA binding activity
Localization
Cell Percentages actin (90%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Arc15

Arc15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arc15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available