Standard name
Human Ortholog
Description Protein of unknown function; involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose; RGI2 has a paralog, RGI1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.97 0.96 0.97 0.89 – 0.81 0.71 0.77 0.72 0.68 0.98 1.0 0.99 0.98 0.98 0.94 0.9 0.86 0.79 0.85 0.82 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.22 0.09 0.36 – 0.39 0.56 0.45 0.55 0.54 0 0 0 0 0 0 0 0 0.09 0.06 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 5 4 1 1 1 2
Bud 0 1 0 2 2 1 – 6 7 9 10 9 0 0 0 0 0 0 2 13 0 1 0 3
Bud Neck 7 1 5 0 3 1 – 5 0 1 2 3 1 0 0 2 0 1 2 3 0 1 0 1
Bud Site 0 0 0 0 0 0 – 1 1 2 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 0 5 2 1 4 – 0 0 4 3 3 0 2 0 5 2 5 4 2 2 0 1 0
Cytoplasm 696 597 285 153 255 277 – 373 314 389 320 254 200 162 199 177 148 142 656 514 197 152 109 111
Endoplasmic Reticulum 4 6 2 1 0 0 – 0 0 0 0 0 3 0 0 5 8 11 2 2 2 0 1 2
Endosome 0 1 0 0 0 1 – 0 0 0 0 0 0 0 0 1 0 0 8 3 2 1 1 2
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 1 2 2 1 0 1 0
Mitochondria 16 6 19 35 24 113 – 179 251 226 243 202 1 0 0 4 3 5 18 16 22 10 3 5
Nucleus 1 2 0 0 0 0 – 1 1 2 1 3 0 0 0 0 0 1 1 2 0 0 0 0
Nuclear Periphery 1 0 1 0 0 0 – 0 1 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 7 0 1 0 1 1 – 3 2 6 6 3 0 0 1 0 0 0 5 6 2 1 0 1
Vac/Vac Membrane 0 4 1 0 0 3 – 2 5 3 3 0 0 0 2 1 1 1 3 10 1 4 6 4
Unique Cell Count 720 611 293 159 263 311 462 445 504 444 372 204 162 202 181 151 151 732 598 249 179 133 141
Labelled Cell Count 741 618 319 193 286 401 570 583 643 589 478 205 164 202 197 163 168 732 598 249 179 133 141


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.6 2.7 3.0 3.4 2.7 – 3.0 2.5 2.7 2.7 2.5 4.6 4.8 4.7 5.5 6.0 5.9 4.6 5.4 5.1
Std Deviation (1e-4) 0.5 1.4 0.6 1.3 1.1 1.1 – 1.3 1.1 0.7 1.2 0.9 1.0 1.4 1.6 1.0 1.3 1.9 1.5 4.1 1.9
Intensity Change (Log2) – – – 0.12 0.32 -0.02 – 0.14 -0.13 -0.03 -0.05 -0.11 0.74 0.82 0.79 1.01 1.12 1.11 0.76 0.97 0.89


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8909 0.0553 -0.406 -0.3888 -0.4915 -0.5749 -0.3366 -0.9393 -0.8163 -0.4727 -0.612 -0.8518 0.406 0.6438 0.6766 -0.0778 0.0914 0.6967
Actin 0.013 0.0004 0.0014 0.0311 0.0001 0.0006 0.0567 0.001 0.0098 0.0765 0.0745 0.0021 0.007 0.0002 0.0002 0.0005 0.0054 0.0001
Bud 0.0002 0.0001 0.0001 0.0002 0 0.0001 0.0128 0.0021 0.0053 0.0174 0.001 0.0003 0.0001 0.0015 0.0009 0.0003 0.0005 0
Bud Neck 0.0034 0.0001 0.0024 0.0097 0 0.0002 0.0058 0.0006 0.0005 0.0009 0.0005 0.0004 0.0008 0.0005 0 0 0.0068 0.0001
Bud Periphery 0.0003 0 0.0002 0.0002 0 0.0001 0.0098 0.0007 0.0103 0.0394 0.0014 0.0007 0.0001 0.0009 0.0008 0.0006 0.0007 0
Bud Site 0.0017 0.0003 0.0028 0.0029 0 0 0.0159 0.0108 0.004 0.0098 0.003 0.0001 0.0003 0.0071 0.0001 0.0001 0.0039 0
Cell Periphery 0.0001 0.0001 0.0002 0.0001 0 0 0.0011 0.0003 0.0007 0.0004 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0
Cytoplasm 0.0617 0.2336 0.1813 0.2427 0.1354 0.2562 0.0494 0.0502 0.0439 0.0754 0.0242 0.0908 0.0496 0.0725 0.0637 0.0612 0.1039 0.106
Cytoplasmic Foci 0.0142 0.0067 0.0058 0.019 0.0106 0.0112 0.0304 0.0049 0.0061 0.0081 0.0133 0.0252 0.0084 0.0021 0.0027 0.0055 0.0375 0.0019
Eisosomes 0.0006 0.0001 0.0001 0.0007 0 0.0001 0.0007 0.0005 0.0003 0.0003 0.0004 0.0001 0.0002 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0033 0.0014 0.0013 0.0012 0.0006 0.0011 0.006 0.0006 0.0052 0.0025 0.0013 0.0016 0.0028 0.0005 0.0004 0.0013 0.0013 0.0004
Endosome 0.0141 0.0031 0.0028 0.0029 0.001 0.002 0.0127 0.0002 0.0053 0.0024 0.0155 0.008 0.0094 0.0004 0.0003 0.0009 0.003 0.0004
Golgi 0.0027 0.0003 0.0007 0.0029 0.0001 0.0001 0.0065 0.0001 0.0014 0.0035 0.0156 0.0027 0.0012 0 0 0.0001 0.0016 0
Lipid Particles 0.0049 0.0002 0.0009 0.0062 0.0006 0.0002 0.0081 0.0006 0.008 0.0016 0.0079 0.0041 0.0022 0.0001 0.0001 0.0003 0.0092 0
Mitochondria 0.001 0.0002 0.0007 0.0011 0.0003 0.0002 0.0047 0.0017 0.0081 0.003 0.0046 0.0013 0.0004 0.0001 0.0005 0.0003 0.0009 0.0002
None 0.8555 0.7509 0.7947 0.6571 0.8493 0.7254 0.7223 0.9157 0.8818 0.7053 0.8278 0.8509 0.9004 0.9111 0.9241 0.9243 0.7853 0.8893
Nuclear Periphery 0.0067 0.0004 0.0009 0.0007 0.0003 0.0003 0.0165 0.0006 0.0023 0.0073 0.0012 0.0016 0.0037 0.0002 0.0004 0.001 0.0019 0.0002
Nucleolus 0.0013 0.0001 0.0003 0.0007 0.0001 0.0001 0.0007 0.0008 0.0004 0.0019 0.0002 0.0002 0.0003 0.0002 0.0004 0.0001 0.002 0
Nucleus 0.0026 0.0007 0.001 0.0008 0.0003 0.0005 0.0203 0.0043 0.0013 0.0213 0.0004 0.0007 0.0014 0.0011 0.0012 0.0012 0.0014 0.0006
Peroxisomes 0.0022 0.0001 0.0003 0.0126 0.0002 0.0002 0.008 0.0011 0.0014 0.0154 0.005 0.0044 0.0037 0.0001 0.0002 0.0003 0.0155 0
Punctate Nuclear 0.009 0.0004 0.0011 0.0064 0.0007 0.0008 0.0082 0.0028 0.0021 0.0034 0.0012 0.004 0.0049 0.0009 0.0036 0.0016 0.0183 0.0003
Vacuole 0.0009 0.0009 0.0008 0.0005 0.0003 0.0004 0.0024 0.0004 0.0012 0.0034 0.0006 0.0006 0.0024 0.0003 0.0002 0.0003 0.0007 0.0002
Vacuole Periphery 0.0005 0.0001 0.0002 0.0001 0 0.0001 0.001 0.0001 0.0005 0.0009 0.0004 0.0003 0.0007 0 0.0001 0.0001 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1693 1655 606 1266 1971 1685 1929 1369 3664 3340 2535 2635

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 613.64 704.01 861.81 793.29 641.53 698.77 759.42 783.72 628.64 701.37 783.90 788.32
Standard Deviation 67.76 86.20 111.19 141.83 129.03 81.41 108.48 138.45 106.17 83.86 117.55 140.17
Intensity Change Log 2 — 0.198203 0.489977 0.370456 — 0.123301 0.243381 0.288822 — 0.160406 0.369206 0.329309

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000090 0.000186 0.000284 0.003927 0.000098 0.000686 0.000246 0.000751 0.000094 0.000438 0.000255 0.002277
Bud Neck 0.018357 0.041895 0.005250 0.009277 0.015655 0.048317 0.009089 0.008982 0.016904 0.045135 0.008171 0.009124
Bud Site 0.005470 0.013358 0.006378 0.056889 0.003153 0.019122 0.017117 0.031878 0.004224 0.016266 0.014550 0.043895
Cell Periphery 0.000153 0.000160 0.000154 0.000323 0.000132 0.000135 0.000111 0.000267 0.000142 0.000148 0.000121 0.000294
Cytoplasm 0.508719* 0.402208* 0.273705* 0.488610* 0.524880* 0.372222* 0.485436* 0.480150* 0.517413* 0.387081* 0.434821* 0.484215*
Cytoplasmic Foci 0.194768* 0.212782* 0.010300 0.024205 0.151940 0.303815* 0.008625 0.027993 0.171729 0.258707* 0.009025 0.026173
Eisosomes 0.000193 0.000113 0.000047 0.000057 0.000128 0.000207 0.000022 0.000057 0.000158 0.000161 0.000028 0.000057
Endoplasmic Reticulum 0.000562 0.000732 0.004117 0.003250 0.000630 0.000345 0.001169 0.001263 0.000598 0.000536 0.001873 0.002218
Endosome 0.007377 0.018689 0.002382 0.005093 0.005502 0.027160 0.001454 0.012223 0.006368 0.022962 0.001676 0.008798
Golgi 0.002080 0.004419 0.000092 0.005091 0.002013 0.010532 0.000311 0.006243 0.002044 0.007503 0.000259 0.005689
Lipid Particles 0.007913 0.005225 0.001584 0.001762 0.006251 0.007875 0.000674 0.001362 0.007019 0.006562 0.000891 0.001554
Mitochondria 0.002638 0.015812 0.000762 0.004454 0.005987 0.014436 0.001604 0.006773 0.004439 0.015118 0.001403 0.005659
Mitotic Spindle 0.000969 0.007019 0.008467 0.038520 0.002556 0.011933 0.014305 0.051566 0.001823 0.009498 0.012909 0.045298
None 0.004280 0.002699 0.007218 0.005583 0.004192 0.002463 0.005546 0.006725 0.004233 0.002580 0.005946 0.006176
Nuclear Periphery 0.000339 0.000536 0.001766 0.001471 0.000526 0.000233 0.001391 0.001265 0.000440 0.000383 0.001481 0.001364
Nuclear Periphery Foci 0.000205 0.000214 0.001201 0.001596 0.000184 0.000226 0.000552 0.000795 0.000194 0.000220 0.000707 0.001180
Nucleolus 0.001087 0.001855 0.000980 0.000854 0.000843 0.001360 0.000306 0.000874 0.000956 0.001605 0.000467 0.000864
Nucleus 0.204232* 0.177960* 0.631820* 0.217880* 0.235944* 0.090756 0.415920* 0.219747* 0.221291* 0.133967* 0.467532* 0.218850*
Peroxisomes 0.003311 0.011073 0.000384 0.001448 0.003108 0.025061 0.000323 0.001832 0.003202 0.018130 0.000338 0.001648
Vacuole 0.036683 0.080347 0.042655 0.123144 0.035718 0.061474 0.035350 0.132168 0.036164 0.070825 0.037096 0.127832
Vacuole Periphery 0.000573 0.002717 0.000457 0.006565 0.000560 0.001641 0.000449 0.007088 0.000566 0.002174 0.000450 0.006837

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.57 -8.66 -2.64 -2.48 -1.99 -3.15 -2.87 -5.28 1.11 -3.07 -3.61 -5.59 -3.28 -2.00 -2.58
Bud Neck -8.41 6.06 7.11 13.57 -0.64 -11.43 4.58 6.31 15.48 1.43 -14.14 7.33 9.46 20.58 0.91
Bud Site -4.95 -0.60 -7.52 -4.93 -6.63 -9.59 -5.72 -7.35 -0.02 -2.27 -10.54 -5.13 -10.43 -3.79 -5.60
Cell Periphery -0.22 -0.90 -1.64 -1.21 -1.07 -0.16 3.16 -1.05 -1.00 -2.08 -0.32 2.50 -1.84 -1.52 -3.21
Cytoplasm 11.57 24.80 11.81 1.10 -13.17 16.16 11.14 11.66 -3.19 1.63 19.66 19.56 16.60 -1.51 -2.83
Cytoplasmic Foci -2.47 38.47 36.48 36.21 -5.37 -21.16 35.64 31.35 46.94 -11.02 -16.98 52.40 47.70 58.06 -12.19
Eisosomes 5.93 9.52 10.19 8.60 1.07 -6.20 14.13 8.69 13.27 -13.37 -0.23 17.19 13.26 15.64 -10.97
Endoplasmic Reticulum -1.44 -13.68 -7.29 -6.97 6.55 2.95 -9.04 -8.99 -14.40 -0.38 0.85 -16.02 -10.32 -11.38 2.50
Endosome -9.37 6.62 6.78 14.93 0.79 -14.02 7.88 -0.67 12.62 -5.80 -16.88 11.09 3.21 18.44 -5.90
Golgi -3.81 6.56 0.42 3.08 -2.53 -7.85 4.00 -0.55 6.51 -3.06 -8.65 6.52 -0.17 7.08 -3.90
Lipid Particles 3.59 8.22 8.91 11.16 1.97 -2.06 9.45 8.47 12.57 -6.42 0.92 13.16 12.27 16.53 -4.10
Mitochondria -7.98 4.71 0.92 8.16 -2.51 -5.34 4.46 2.27 7.79 -2.01 -9.23 5.37 2.49 11.23 -2.60
Mitotic Spindle -3.80 -2.60 -7.71 -5.35 -4.24 -4.67 -5.32 -7.62 -4.70 -4.24 -5.95 -6.30 -10.76 -7.06 -5.61
None 2.34 -1.37 -0.03 -2.48 1.39 1.93 -1.06 -1.14 -3.35 -0.44 2.88 -1.75 -1.02 -4.16 0.60
Nuclear Periphery -4.69 -20.10 -16.28 -14.95 0.91 6.25 -12.94 -14.66 -18.71 0.56 1.59 -18.40 -21.83 -23.53 0.31
Nuclear Periphery Foci -1.11 -7.48 -5.68 -5.51 1.79 -0.95 -4.60 -8.94 -5.52 -1.62 -1.27 -7.97 -8.61 -7.55 -1.01
Nucleolus -3.61 0.72 3.04 6.17 2.18 -3.46 6.51 0.63 2.80 -1.72 -5.09 5.73 2.37 6.06 -0.98
Nucleus 3.74 -35.64 -5.74 -8.71 29.92 23.89 -26.26 -2.11 -22.23 21.74 18.63 -40.51 -5.25 -21.20 33.11
Peroxisomes -8.03 9.32 7.98 11.32 -2.13 -13.93 9.27 5.93 15.48 -3.16 -15.95 13.13 9.65 18.90 -3.93
Vacuole -12.73 -7.43 -27.58 -20.52 -18.61 -8.80 -7.79 -27.46 -23.24 -23.11 -15.36 -10.71 -38.89 -30.75 -31.18
Vacuole Periphery -9.51 1.51 -2.39 0.49 -2.64 -7.31 1.66 -3.00 -1.59 -3.29 -11.92 2.12 -3.83 -0.82 -4.22
Standard name
Human Ortholog
Description Protein of unknown function; involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose; RGI2 has a paralog, RGI1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (40%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rgi2

Rgi2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgi2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available