Standard name
Human Ortholog
Description Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.06 0.05 0.06 0.17 0.28 0.32 0.34 0.34 – 0.29 0.36 0.33 0.06 0.07 0.05 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.05 – 0.05 0.06 0.07 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0.11 0.11 0.16 0 0.05 0 – 0 0 0 0 0 0.05 – – – 0 0.06 0.07 0.07 0.07 0.07
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.07 0 0.12 0.06 0.3 0.42 0.31 – 0.47 0.43 0.38 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.07 0.05 0 0 0 0 0 0 – 0 0 0 0 0.07 0.07 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0.08 0 0 0 0 0 – 0 0 0.05 0 0.05 0 – – – 0 0 0.12 0.07 0.05 0.06
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.9 0.87 0.84 0.7 0.64 0.51 0.35 0.41 – 0.38 0.33 0.37 0.93 0.88 0.91 – – – 0.85 0.84 0.75 0.79 0.82 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 0 0 2 – 0 3 1 2 0 1 – – – 1 1 3 0 0 1
Bud 0 3 0 0 1 3 5 3 – 10 3 8 0 0 1 – – – 2 8 5 1 0 4
Bud Neck 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 2 1 1 3 – 5 15 16 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 25 36 38 54 95 174 186 241 – 221 201 182 10 22 17 – – – 2 5 2 0 0 2
Endoplasmic Reticulum 1 16 28 12 5 23 35 33 – 40 36 39 2 7 6 – – – 1 2 7 2 2 2
Endosome 6 15 68 35 55 15 26 31 – 15 4 7 5 10 17 – – – 15 43 50 12 20 23
Golgi 4 2 17 12 1 2 1 3 – 1 1 2 0 4 3 – – – 2 1 5 0 2 1
Mitochondria 2 46 12 38 21 165 227 219 – 356 243 209 0 0 0 – – – 6 10 9 2 2 2
Nucleus 31 33 7 2 3 3 4 9 – 4 0 4 3 22 21 – – – 7 9 1 2 6 5
Nuclear Periphery 11 22 53 7 2 7 15 12 – 15 11 26 5 15 14 – – – 19 24 78 13 13 20
Nucleolus 3 6 3 1 4 1 4 2 – 5 3 3 0 2 2 – – – 3 1 0 0 0 1
Peroxisomes 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 2 1 1 1 1 0 0 – 1 0 0 0 0 0 – – – 1 1 0 1 0 0
Vac/Vac Membrane 389 587 543 223 218 283 188 291 – 283 185 204 166 277 289 – – – 400 613 509 149 236 267
Unique Cell Count 431 676 643 319 340 552 543 715 754 566 554 179 316 319 469 727 680 189 290 335
Labelled Cell Count 472 769 771 385 410 678 692 849 956 705 701 193 359 371 469 727 680 189 290 335


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 8.3 8.5 7.8 6.8 6.0 5.8 6.7 – 6.4 7.1 7.4 10.2 10.4 10.0 – – – 11.1 11.5 11.7
Std Deviation (1e-4) 1.9 1.7 1.5 1.5 1.4 1.5 1.4 1.3 – 1.4 1.5 1.5 1.6 1.6 1.6 – – – 2.3 2.1 2.3
Intensity Change (Log2) – – – -0.13 -0.31 -0.49 -0.55 -0.34 – -0.4 -0.25 -0.21 0.26 0.3 0.24 – – – 0.38 0.43 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 – 0 4.2 4.3 0 0 0 – – –
Cytoplasm 5.5 9.6 11.6 12.4 12.7 – 11.2 12.9 12.0 -0.2 0.6 -0.4 – – –
Endoplasmic Reticulum -0.4 -2.4 -0.2 1.6 0.2 – 0.8 1.6 2.0 -2.0 -1.7 -2.0 – – –
Endosome 0.2 2.5 -5.3 -3.7 -4.4 – -6.8 -7.2 -6.6 -3.2 -3.9 -2.7 – – –
Golgi 1.0 -2.6 -3.1 -3.5 -3.4 – -4.1 -3.5 -3.2 0 -1.4 -1.7 – – –
Mitochondria 6.6 3.6 13.6 17.1 14.1 – 19.2 17.5 15.9 0 0 0 – – –
Nucleus 0 0 0 -0.6 0.3 – -1.2 0 -0.7 0 5.0 4.8 – – –
Nuclear Periphery -3.7 -5.0 -5.5 -4.0 -5.7 – -5.4 -4.9 -2.5 -2.5 -2.0 -2.2 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole -5.3 -7.3 -12.4 -17.6 -16.5 – -17.8 -18.3 -17.0 2.8 1.3 2.6 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.4107 6.5345 6.2156 6.0584 5.4634 6.202 7.5435 8.7788 8.3442 9.0017 7.2686 8.2113 8.2065 9.0845 8.5891 8.7444 9.1727 8.5387
Actin 0.0002 0.0001 0.0001 0 0 0 0.0136 0 0.0022 0 0.0005 0.0011 0.0007 0 0.0001 0 0.0107 0
Bud 0.0005 0.0016 0.0007 0.0011 0.0006 0.0003 0.0004 0.0002 0.0006 0.0001 0.0001 0.0005 0.001 0.0003 0.0005 0.0001 0.0005 0.0006
Bud Neck 0.0002 0.0003 0.0007 0.0003 0.0002 0.0003 0.0008 0.0002 0.0005 0.0001 0 0.0004 0.0024 0.0003 0.0004 0.0001 0.0003 0.0004
Bud Periphery 0.0009 0.0014 0.0012 0.0024 0.001 0.0007 0.001 0.0007 0.0016 0.0002 0.0002 0.0025 0.002 0.0004 0.0008 0.0002 0.0006 0.0012
Bud Site 0.0004 0.0036 0.0027 0.0006 0.0006 0.0002 0.0012 0.0004 0.0009 0.0001 0.0001 0.0003 0.0056 0.0004 0.0009 0.0001 0.0012 0.0004
Cell Periphery 0.0008 0.001 0.0006 0.0006 0.0004 0.0005 0.0013 0.0008 0.0014 0.0001 0.0002 0.0007 0.0026 0.001 0.0009 0.0003 0.0004 0.001
Cytoplasm 0.0066 0.0315 0.0079 0.0044 0.0057 0.006 0.0102 0.0082 0.0072 0.0007 0.0003 0.0023 0.0084 0.0181 0.0092 0.0005 0.0033 0.0021
Cytoplasmic Foci 0.002 0.0056 0.004 0.0013 0.003 0.0028 0.029 0.0011 0.0038 0.0003 0.0053 0.0032 0.0033 0.0061 0.0032 0.0002 0.0098 0.0099
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0044 0.0055 0.0115 0.0015 0.0019 0.004 0.0106 0.0034 0.0071 0.0023 0.0009 0.0025 0.0048 0.0054 0.0078 0.0006 0.0096 0.0029
Endosome 0.0841 0.0722 0.1214 0.0887 0.0749 0.0806 0.1065 0.048 0.1192 0.0695 0.0857 0.0787 0.1212 0.0458 0.1331 0.0441 0.0824 0.0494
Golgi 0.0017 0.0012 0.0039 0.0021 0.0014 0.0009 0.008 0.0006 0.0083 0.0007 0.0086 0.007 0.0057 0.0005 0.0041 0.0002 0.0038 0.0016
Lipid Particles 0.0022 0.0027 0.0019 0.0012 0.0005 0.0006 0.0296 0.0008 0.0056 0.0002 0.0235 0.0071 0.0067 0.0037 0.0013 0.0001 0.0065 0.0059
Mitochondria 0.0054 0.0024 0.0106 0.0023 0.0017 0.0016 0.0064 0.0025 0.0412 0.0007 0.0029 0.0092 0.0084 0.0011 0.0085 0.0002 0.0021 0.0024
None 0.0004 0.0029 0.0003 0.0001 0.0001 0.0001 0.0023 0.0003 0.0007 0 0.0002 0.0001 0.0006 0.0013 0.0002 0 0.0004 0.0005
Nuclear Periphery 0.0285 0.0215 0.0486 0.0053 0.0133 0.0324 0.0505 0.0254 0.0354 0.0097 0.0132 0.0146 0.0145 0.0274 0.0414 0.0037 0.0297 0.0293
Nucleolus 0.0026 0.0037 0.0013 0.0006 0.0008 0.0019 0.004 0.0011 0.0012 0.0001 0.0002 0.0012 0.0204 0.0031 0.0049 0.0002 0.0085 0.005
Nucleus 0.0149 0.0111 0.0122 0.002 0.0024 0.0104 0.013 0.0186 0.0117 0.0007 0.0006 0.0037 0.0234 0.0149 0.0105 0.0009 0.004 0.0064
Peroxisomes 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0213 0.0001 0.0015 0 0.0033 0.0013 0.0007 0.0001 0.0001 0 0.0272 0.0002
Punctate Nuclear 0.0001 0.0006 0.0001 0 0.0001 0.0001 0.0316 0 0.0038 0 0.0016 0.0001 0.0009 0.0001 0.0001 0 0.0006 0.008
Vacuole 0.4735 0.5492 0.4594 0.5077 0.3511 0.4027 0.3889 0.4741 0.4145 0.4549 0.2407 0.4097 0.4913 0.5036 0.4841 0.4996 0.443 0.4422
Vacuole Periphery 0.3705 0.2818 0.311 0.3778 0.5404 0.4538 0.2699 0.4134 0.3315 0.4596 0.6118 0.4538 0.2757 0.3664 0.2881 0.449 0.3555 0.4305

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.2637 67.7246 59.9813 52.2552 62.0513 52.9211 74.7349 62.1195 60.5715 71.8069
Translational Efficiency 0.3602 0.3285 0.3376 0.3412 0.368 0.2992 0.3062 0.3746 0.3123 0.2937

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
167 1577 1436 922 1580 1773 1354 1584 1747 3350 2790 2506

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 978.76 953.61 1254.32 1123.59 869.41 1023.56 1240.75 1265.64 879.86 990.63 1247.73 1213.38
Standard Deviation 161.09 200.88 210.79 212.91 153.64 151.69 209.82 190.68 157.68 179.98 210.43 210.60
Intensity Change Log 2 — -0.037556 0.357878 0.199089 — 0.235487 0.513104 0.541759 — 0.097339 0.432983 0.370448

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001044 0.000040 0.000074 0.000120 0.000020 0.000023 0.000042 0.000068 0.000118 0.000031 0.000059 0.000087
Bud Neck 0.005865 0.000827 0.004869 0.001617 0.000921 0.000441 0.001181 0.001139 0.001394 0.000622 0.003079 0.001315
Bud Site 0.035809 0.007575 0.004664 0.013033 0.003763 0.001022 0.002960 0.005992 0.006826 0.004107 0.003837 0.008582
Cell Periphery 0.000458 0.000330 0.000413 0.000790 0.000151 0.000197 0.000224 0.000465 0.000180 0.000259 0.000321 0.000585
Cytoplasm 0.058114 0.009644 0.001595 0.005541 0.006125 0.000842 0.001566 0.002171 0.011095 0.004985 0.001581 0.003411
Cytoplasmic Foci 0.055274 0.008122 0.000942 0.007111 0.007279 0.001622 0.002001 0.003968 0.011867 0.004682 0.001456 0.005124
Eisosomes 0.000044 0.000004 0.000033 0.000007 0.000004 0.000004 0.000007 0.000005 0.000008 0.000004 0.000021 0.000006
Endoplasmic Reticulum 0.008722 0.002366 0.002913 0.004153 0.003432 0.006161 0.001401 0.003493 0.003937 0.004375 0.002179 0.003736
Endosome 0.102927 0.026901 0.012969 0.043201 0.028510 0.012881 0.013205 0.032487 0.035624 0.019481 0.013083 0.036429
Golgi 0.035856 0.006009 0.002655 0.020834 0.002511 0.001465 0.007116 0.014174 0.005699 0.003604 0.004820 0.016624
Lipid Particles 0.004667 0.001121 0.000641 0.001000 0.000498 0.000613 0.000432 0.000416 0.000897 0.000853 0.000540 0.000631
Mitochondria 0.008686 0.004591 0.001873 0.001429 0.003256 0.006346 0.001129 0.001569 0.003775 0.005519 0.001512 0.001517
Mitotic Spindle 0.016350 0.000282 0.000619 0.000788 0.000115 0.000370 0.000376 0.000518 0.001667 0.000328 0.000501 0.000618
None 0.000384 0.001114 0.000187 0.000112 0.000595 0.000196 0.000093 0.000077 0.000575 0.000628 0.000141 0.000090
Nuclear Periphery 0.001745 0.000848 0.004701 0.000749 0.000433 0.003219 0.002258 0.000700 0.000558 0.002102 0.003516 0.000718
Nuclear Periphery Foci 0.003287 0.001160 0.002078 0.001891 0.000327 0.001520 0.002642 0.001768 0.000610 0.001351 0.002352 0.001813
Nucleolus 0.005692 0.001905 0.012614 0.000906 0.000463 0.001346 0.002351 0.000539 0.000963 0.001609 0.007633 0.000674
Nucleus 0.013271 0.001546 0.010954 0.001109 0.001477 0.002016 0.001524 0.000425 0.002604 0.001795 0.006378 0.000676
Peroxisomes 0.001544 0.000282 0.000410 0.000321 0.000221 0.000235 0.000144 0.000108 0.000347 0.000257 0.000281 0.000187
Vacuole 0.583348* 0.327027* 0.504829* 0.637242* 0.405777* 0.409652* 0.523817* 0.638074* 0.422751* 0.370756* 0.514044* 0.637768*
Vacuole Periphery 0.056914 0.598306* 0.429966* 0.258045* 0.534122* 0.549829* 0.435532* 0.291845* 0.488505* 0.572649* 0.432667* 0.279409*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.60 1.54 1.45 -2.58 -1.50 -0.54 -1.98 -2.01 -1.89 -0.96 1.43 0.94 0.41 -3.01 -1.46
Bud Neck 4.69 0.72 3.90 -3.15 4.81 2.63 -1.33 -1.14 -5.12 0.21 3.62 -4.44 0.17 -5.07 4.94
Bud Site 3.48 3.85 2.71 -2.84 -4.53 4.61 0.98 -2.51 -6.41 -3.37 2.48 2.73 -1.65 -4.85 -5.21
Cell Periphery 0.65 0.19 -1.22 -2.04 -1.68 -1.66 -2.39 -4.76 -4.04 -3.58 -2.28 -3.94 -4.30 -3.44 -2.79
Cytoplasm 4.75 5.53 5.10 2.25 -4.09 5.00 3.78 3.46 -3.06 -1.17 4.12 6.54 5.12 1.48 -3.55
Cytoplasmic Foci 6.79 7.84 6.92 0.94 -7.76 9.53 8.22 4.77 -5.08 -3.75 7.47 11.23 6.91 -0.91 -8.34
Eisosomes 4.57 1.13 4.24 -3.46 7.35 -0.31 -3.49 -1.63 -1.15 2.50 3.49 -6.20 2.03 -2.95 7.89
Endoplasmic Reticulum 2.65 2.41 1.81 -2.52 -1.73 -3.64 5.43 -0.11 3.58 -5.65 -0.85 4.14 0.35 1.27 -4.27
Endosome 8.10 9.71 6.15 -4.88 -9.83 7.75 7.28 -1.53 -9.92 -9.35 7.45 10.75 -0.36 -9.39 -13.53
Golgi 3.29 3.68 1.58 -6.94 -8.72 1.72 -4.48 -7.00 -7.92 -3.88 1.93 0.72 -6.86 -10.09 -8.82
Lipid Particles 1.94 2.32 2.04 0.11 -0.92 -0.73 0.44 0.70 1.40 0.09 0.13 1.78 1.01 0.62 -0.61
Mitochondria 1.23 2.16 2.31 3.61 1.10 -2.87 3.13 2.36 5.18 -1.10 -2.00 3.32 3.25 6.22 0.01
Mitotic Spindle 2.12 2.08 2.05 -1.89 -0.60 -1.91 -2.60 -2.35 -1.32 -1.32 1.83 1.57 1.18 -2.18 -1.21
None -1.44 0.66 0.85 3.42 1.98 1.80 2.35 2.40 1.85 0.52 -0.21 2.23 2.47 3.79 2.05
Nuclear Periphery 1.36 -2.99 1.52 0.30 4.75 -5.24 -6.19 -1.51 4.50 4.59 -4.97 -6.68 -1.08 4.15 6.11
Nuclear Periphery Foci 1.77 0.99 1.10 -2.10 0.32 -2.99 -4.23 -2.78 -0.41 1.15 -2.82 -5.18 -3.30 -1.19 1.11
Nucleolus 1.15 -1.82 1.46 1.37 8.38 -3.13 -4.80 -0.43 2.55 4.33 -1.30 -8.27 0.83 2.49 9.31
Nucleus 2.33 0.44 2.38 0.42 7.67 -0.92 -0.14 2.52 3.62 5.10 1.24 -4.45 2.90 3.13 8.80
Peroxisomes 5.35 4.58 4.69 -0.30 0.75 -0.25 1.78 3.09 2.49 1.07 1.90 1.07 2.77 1.31 1.69
Vacuole 10.39 3.20 -2.10 -33.81 -13.64 -0.47 -14.06 -28.41 -27.99 -13.75 7.24 -12.36 -28.94 -43.85 -19.65
Vacuole Periphery -52.33* -35.63* -19.27 35.42* 17.69* -1.73 10.71 27.64* 30.53* 16.68* -10.10 6.72 25.55 45.98* 23.64*
Standard name
Human Ortholog
Description Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency
Localization
Cell Percentages vacuolar membrane (67%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Syg1

Syg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Syg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available