Standard name
Human Ortholog
Description Subunit of the cohesin complex; which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms; relocalizes to the cytosol in response to hypoxia; essential for viability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 – 0 0 0 0 0.06 0 0.05 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 – 0.11 0 0 0 0 0 0 0 0.06 0.15 0.16 0.11 0.09 0 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.17 0.16 – 0.05 0.13 0.16 0.14 0.15 0.22 0.15 0.22 0 0 0 0 0.06 0 0 0 0 0 0.06 0.05
Nucleus – 0.65 0.4 – 0.32 0.4 0.45 0.41 0.4 0.5 0.5 0.52 0.73 0.59 0.58 0.58 0.59 0.64 0.54 0.53 0.21 0.37 0.43 0.43
Nuclear Periphery – 0.07 0.16 – 0.22 0.23 0.26 0.2 0.15 0.16 0.35 0.22 0.07 0 0 0.16 0.18 0.09 0.18 0.08 0.27 0.27 0.22 0.13
Nucleolus – 0.21 0.32 – 0.32 0.21 0.27 0.32 0.33 0.28 0.1 0.24 0.21 0.27 0.23 0.21 0.11 0.17 0.14 0.19 0.29 0.14 0 0
Peroxisomes – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0
Vac/Vac Membrane – 0.13 0.08 – 0.16 0.32 0.21 0.11 0.15 0.17 0.3 0.12 0.06 0 0 0.14 0.14 0.14 0.05 0.06 0.08 0.1 0.18 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud – 0 0 – 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 5 1 2 3 4
Bud Neck – 0 1 – 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 2 1
Bud Site – 0 0 – 0 1 2 0 3 1 1 4 0 0 2 1 0 0 – – – – – –
Cell Periphery – 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm – 8 1 – 4 2 1 2 0 2 0 1 14 39 53 15 13 5 0 4 0 0 3 0
Endoplasmic Reticulum – 0 0 – 0 0 0 0 0 0 0 0 1 0 1 0 3 3 0 0 0 0 4 4
Endosome – 1 0 – 0 1 0 1 0 0 0 0 0 3 4 0 2 1 1 5 2 6 5 8
Golgi – 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 1
Mitochondria – 38 4 – 2 12 18 16 8 22 3 11 1 1 0 2 8 5 0 3 0 5 22 22
Nucleus – 149 10 – 12 36 52 47 21 50 10 26 160 148 197 81 86 89 15 129 11 65 164 193
Nuclear Periphery – 15 4 – 8 21 30 23 8 16 7 11 15 11 13 22 26 13 5 18 15 48 84 59
Nucleolus – 49 8 – 12 19 31 37 17 28 2 12 47 67 77 29 16 23 4 46 16 24 15 15
Peroxisomes – 0 0 – 0 0 0 0 0 0 0 0 0 1 4 0 0 0 0 0 0 0 0 0
SpindlePole – 0 1 – 0 0 0 0 0 0 0 0 0 11 14 1 0 0 0 14 2 2 3 4
Vac/Vac Membrane – 31 2 – 6 29 24 12 8 17 6 6 13 8 10 19 20 19 1 14 4 17 68 131
Unique Cell Count 230 25 37 91 116 114 52 101 20 50 220 252 337 140 145 139 29 244 56 177 384 452
Labelled Cell Count 291 31 44 121 159 139 65 136 30 71 251 290 377 170 174 158 29 244 56 177 384 452


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.7 5.9 – 6.6 6.1 6.2 7.2 6.4 6.2 6.4 5.7 8.4 8.6 8.7 9.8 9.5 9.9 6.6 6.5 6.8
Std Deviation (1e-4) – 1.3 1.3 – 1.3 1.4 1.4 1.7 1.6 1.3 1.4 1.2 2.1 2.8 2.9 2.6 2.6 2.5 1.1 1.1 1.3
Intensity Change (Log2) – – – – 0.16 0.05 0.07 0.27 0.11 0.07 0.11 -0.07 0.5 0.54 0.55 0.73 0.68 0.73 0.14 0.13 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0.6 0 0.6 0 0 0 0 0 0
Nucleus – -0.6 0 0.4 0.1 0 0.9 0.7 1.0 3.4 1.8 1.8 1.7 1.8 2.3
Nuclear Periphery – 0 0.8 1.0 0 0 0 0 0.6 0 0 0 0 0 0
Nucleolus – 0 -1.2 -0.5 0 0.1 -0.4 0 -0.7 -1.2 -0.6 -1.0 -1.2 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole – 0 2.4 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3359 2.5313 3.4684 2.707 2.5282 2.4124 3.6083 4.489 4.8305 3.7291 3.6129 4.0436 2.4969 3.2437 3.4629 3.2504 3.2378 2.8545
Actin 0.0003 0.0003 0.0001 0.0001 0.001 0.0004 0.0204 0.0001 0.0029 0.0392 0.0279 0.0037 0.0143 0.0001 0.0004 0 0.0014 0.001
Bud 0.0001 0.0001 0 0 0.0001 0 0.0003 0 0.0001 0.0002 0.0002 0 0.0001 0 0 0.0001 0.0003 0.0001
Bud Neck 0.0002 0.0002 0.0001 0.0001 0.0002 0.001 0.0016 0.0001 0.0002 0.0027 0.0005 0.0011 0.0011 0.0001 0.0002 0.0003 0.0013 0.0015
Bud Periphery 0.0001 0.0001 0 0 0.0001 0.0002 0.0005 0 0.0001 0.0003 0.0003 0.0002 0.0003 0 0.0001 0 0.0026 0.0004
Bud Site 0.0001 0.0003 0 0 0.0001 0 0.0011 0.0001 0.0002 0.0028 0.0003 0 0.0008 0 0.0001 0 0.0007 0.0001
Cell Periphery 0 0.0001 0 0 0 0.0001 0.0002 0 0 0.0002 0.0001 0 0.0001 0 0 0 0.0005 0.0001
Cytoplasm 0.0004 0.0085 0 0 0.0002 0.0001 0.0096 0.0007 0.001 0.0011 0.0007 0.0001 0.0124 0 0.0002 0.0001 0.0005 0.0002
Cytoplasmic Foci 0.0001 0.0012 0 0 0 0 0.0048 0 0.0046 0.0088 0.0043 0.0001 0.0097 0 0 0 0.0014 0
Eisosomes 0 0.0002 0 0 0 0 0.0002 0 0.0001 0.0005 0.0001 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0002 0.0006 0 0 0.0002 0.0002 0.0013 0.0002 0.0006 0.0019 0.001 0.0004 0.001 0 0.0003 0 0.0011 0.0005
Endosome 0.0003 0.0015 0 0 0.0005 0.0003 0.0055 0.0002 0.0072 0.0146 0.0088 0.001 0.0037 0 0.0003 0 0.0044 0.0004
Golgi 0 0.0001 0 0 0.0001 0.0001 0.0031 0 0.003 0.0081 0.0077 0.0003 0.0014 0 0 0 0.0007 0.0001
Lipid Particles 0.0001 0.0015 0.0001 0 0.0002 0.0004 0.0067 0 0.0067 0.0304 0.0057 0.0005 0.0128 0 0.0001 0 0.0081 0.0007
Mitochondria 0.001 0.0006 0.0002 0.0002 0.0014 0.0009 0.0033 0.0002 0.0049 0.0215 0.0037 0.0006 0.0026 0.0004 0.0004 0.0002 0.0137 0.0012
None 0.0002 0.0406 0 0 0.0002 0.0001 0.0064 0.0001 0.0016 0.001 0.0016 0.0002 0.0039 0 0.0001 0 0.001 0.0002
Nuclear Periphery 0.1812 0.1534 0.0401 0.0035 0.2735 0.3396 0.2392 0.064 0.0408 0.0044 0.2938 0.4026 0.2281 0.0467 0.0453 0.0037 0.3887 0.3399
Nucleolus 0.0481 0.0444 0.106 0.0974 0.0223 0.0921 0.042 0.0442 0.0786 0.0491 0.075 0.0779 0.0372 0.0414 0.0872 0.0368 0.0801 0.0788
Nucleus 0.7529 0.7239 0.8286 0.8951 0.6924 0.5468 0.6176 0.8758 0.8143 0.7589 0.5308 0.4968 0.6481 0.9087 0.8554 0.9573 0.4609 0.5639
Peroxisomes 0 0.0001 0 0 0 0 0.005 0 0.0019 0.0168 0.0033 0.0001 0.0036 0 0 0 0.0007 0.0001
Punctate Nuclear 0.0123 0.0183 0.024 0.0033 0.0042 0.0118 0.0278 0.0134 0.0289 0.0335 0.0295 0.0107 0.0131 0.0019 0.0092 0.0013 0.0121 0.0054
Vacuole 0.0004 0.002 0.0001 0 0.0002 0.0007 0.0006 0.0002 0.0007 0.0019 0.0006 0.0005 0.0008 0.0001 0.0001 0 0.0056 0.0007
Vacuole Periphery 0.0019 0.002 0.0003 0.0001 0.0031 0.0051 0.003 0.0006 0.0013 0.0019 0.0043 0.003 0.0049 0.0004 0.0005 0.0001 0.0138 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.8685 17.1395 7.419 21.1583 35.8378 54.9321 16.5354 12.7685 17.0443 46.4305
Translational Efficiency 0.9077 0.6534 0.6684 0.663 0.8521 1.0729 0.6744 0.4805 0.5801 0.6899

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1781 931 2195 140 2234 2813 1834 2649 4015 3744 4029 2789

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 759.50 872.69 974.49 1068.03 802.98 852.83 954.39 988.26 783.69 857.77 965.34 992.26
Standard Deviation 100.16 158.79 162.78 166.03 123.38 121.17 149.21 157.63 115.70 131.82 157.06 159.01
Intensity Change Log 2 — 0.200419 0.359597 0.491830 — 0.086894 0.249215 0.299527 — 0.143193 0.303926 0.396206

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000454 0.001187 0.000590 0.001946 0.000397 0.000523 0.000812 0.000773 0.000422 0.000688 0.000691 0.000832
Bud Neck 0.002310 0.005173 0.014721 0.012913 0.003230 0.004953 0.018002 0.021596 0.002821 0.005008 0.016215 0.021160
Bud Site 0.000499 0.002012 0.000831 0.002666 0.000403 0.000741 0.001459 0.002582 0.000445 0.001057 0.001117 0.002586
Cell Periphery 0.000097 0.000137 0.000041 0.000093 0.000074 0.000070 0.000063 0.000088 0.000085 0.000086 0.000051 0.000088
Cytoplasm 0.015335 0.009607 0.000972 0.002678 0.005341 0.002514 0.002425 0.003720 0.009774 0.004277 0.001633 0.003668
Cytoplasmic Foci 0.003635 0.002637 0.000024 0.000599 0.001106 0.000597 0.000404 0.000338 0.002228 0.001104 0.000197 0.000351
Eisosomes 0.000037 0.000037 0.000019 0.000050 0.000025 0.000023 0.000027 0.000026 0.000031 0.000026 0.000023 0.000027
Endoplasmic Reticulum 0.007007 0.007790 0.001795 0.009339 0.005148 0.007356 0.002671 0.003292 0.005973 0.007464 0.002194 0.003596
Endosome 0.001910 0.003285 0.000335 0.000671 0.001733 0.001604 0.000613 0.000769 0.001812 0.002022 0.000462 0.000764
Golgi 0.001137 0.001948 0.000273 0.000381 0.000610 0.000861 0.000451 0.000468 0.000844 0.001132 0.000354 0.000463
Lipid Particles 0.008316 0.001146 0.000025 0.000593 0.001480 0.001333 0.000085 0.000130 0.004512 0.001287 0.000052 0.000153
Mitochondria 0.004827 0.006604 0.001940 0.003059 0.003153 0.007399 0.003407 0.002407 0.003896 0.007201 0.002608 0.002440
Mitotic Spindle 0.011685 0.010733 0.012289 0.065396 0.006985 0.010135 0.017323 0.015435 0.009070 0.010284 0.014581 0.017943
None 0.013632 0.012030 0.001839 0.003234 0.007355 0.003318 0.002579 0.004660 0.010140 0.005484 0.002176 0.004588
Nuclear Periphery 0.069862 0.031020 0.015101 0.007427 0.055645 0.089048 0.019574 0.011976 0.061951 0.074618 0.017137 0.011747
Nuclear Periphery Foci 0.114135 0.086044 0.007568 0.012258 0.078395 0.076239 0.013752 0.009286 0.094249 0.078677 0.010383 0.009435
Nucleolus 0.294015* 0.240580* 0.263552* 0.218739 0.308709* 0.236261* 0.288811* 0.199870 0.302191* 0.237335* 0.275050* 0.200817
Nucleus 0.447834* 0.565414* 0.676451* 0.650044* 0.517042* 0.551880* 0.624397* 0.720016* 0.486342* 0.555245* 0.652756* 0.716504*
Peroxisomes 0.001228 0.001785 0.000597 0.003841 0.000834 0.000839 0.001228 0.000662 0.001009 0.001074 0.000884 0.000821
Vacuole 0.001207 0.005143 0.000493 0.003612 0.001434 0.002500 0.001015 0.001267 0.001333 0.003157 0.000731 0.001385
Vacuole Periphery 0.000836 0.005690 0.000545 0.000461 0.000900 0.001806 0.000898 0.000640 0.000871 0.002772 0.000706 0.000631

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.14 -5.57 -8.69 -5.01 -7.97 -4.36 -12.88 -13.97 -9.12 0.52 -9.14 -14.05 -15.04 -6.31 -5.33
Bud Neck -6.46 -20.82 -8.50 -6.81 -0.87 -5.01 -18.05 -21.99 -20.03 -3.38 -8.27 -26.92 -23.42 -20.69 -5.87
Bud Site -4.85 -4.17 -5.08 -2.79 -4.49 -1.83 -7.22 -5.77 -4.88 -2.28 -4.04 -8.58 -6.08 -4.48 -3.79
Cell Periphery -2.91 7.88 -0.63 1.85 -5.19 0.60 1.32 -1.26 -1.64 -2.41 -0.17 6.38 -0.18 -0.01 -4.77
Cytoplasm 2.09 9.29 6.19 3.34 -1.99 3.68 3.48 1.77 -2.32 -2.15 5.71 9.82 6.55 0.82 -4.02
Cytoplasmic Foci 1.25 6.25 4.61 2.86 -2.58 2.30 2.54 3.16 2.00 0.22 3.61 6.77 6.31 3.95 -1.03
Eisosomes 1.01 13.78 -3.62 -3.94 -7.60 2.59 -3.08 -1.31 -4.32 1.98 5.14 8.08 2.56 -2.49 -4.93
Endoplasmic Reticulum -1.21 12.40 -1.38 -0.85 -4.40 -4.87 7.63 5.48 9.70 -2.62 -3.95 14.82 7.36 10.19 -6.61
Endosome -1.79 6.02 2.57 3.06 -2.08 0.46 3.31 2.81 3.23 -0.95 -0.49 6.04 4.26 4.55 -2.17
Golgi -1.76 3.38 2.36 3.92 -4.62 -0.58 0.97 0.89 1.15 0.01 -0.83 3.41 2.31 2.51 -0.83
Lipid Particles 8.87 10.52 9.30 1.64 -3.51 0.53 6.57 6.35 4.57 -1.84 7.64 11.98 11.66 5.59 -5.12
Mitochondria -1.76 7.01 2.57 3.17 -3.02 -7.09 -0.19 2.72 8.30 1.40 -6.33 4.40 5.58 9.17 -0.22
Mitotic Spindle 0.41 -0.73 -4.14 -4.19 -4.06 -1.57 -5.99 -5.69 -4.42 0.47 -0.41 -4.89 -5.99 -5.69 -2.49
None 0.76 10.50 9.06 5.88 -1.86 5.27 6.60 3.40 -1.94 -3.34 5.95 12.45 7.34 1.52 -4.23
Nuclear Periphery 7.41 13.13 14.88 7.22 3.74 -7.37 11.08 13.58 21.90* 3.03 -3.89 17.27 18.93 22.10 2.99
Nuclear Periphery Foci 3.41 20.78* 18.86* 10.64 -0.96 0.44 15.89 17.16 18.98* 1.85 3.64 26.10 25.88 21.66 0.68
Nucleolus 6.51 1.98 2.94 0.21 2.21 10.90 -0.13 13.08 3.01 12.29 12.71 1.64 14.36 3.32 12.87
Nucleus -10.06 -22.85 -5.53 -1.08 2.19 -4.40 -9.43 -21.96 -18.05 -11.39 -10.57 -22.70 -29.37 -18.65 -9.12
Peroxisomes -2.40 5.08 -3.77 -3.13 -4.68 0.99 -4.13 1.60 0.46 7.58 0.54 0.65 1.80 1.22 1.63
Vacuole -4.41 2.39 -2.54 -1.10 -2.82 -1.57 0.78 -0.23 1.62 -1.36 -3.59 2.04 -1.49 2.39 -3.64
Vacuole Periphery -4.40 2.30 2.70 4.68 -0.16 -2.63 0.43 1.42 4.29 1.16 -5.22 1.51 1.94 6.23 0.43
Standard name
Human Ortholog
Description Subunit of the cohesin complex; which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms; relocalizes to the cytosol in response to hypoxia; essential for viability
Localization
Cell Percentages nucleus (37%), nuclear periphery (19%), mixed (31%)
Cell Cycle Regulation Yes
nuclear periphery - T/G1 (4.4e-08)
nucleus - S/G2 (1.1e-10)
Subcompartmental Group N/A

Irr1

Irr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Irr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available