Standard name
Human Ortholog
Description Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ("nineteen complex") and acts after Prp2p to promote the first catalytic reaction of splicing
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.08 0.12 0.12 0.08 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.29 0 0 0 0.1 0.12 0.11 0.38 0.29 0.3 0.38 0.05 0 0 0.05 0 0.06 0 0 0 0.06 0 0.06
Nucleus 0.9 0.92 0.94 0.95 0.95 0.89 0.91 0.87 0.83 0.83 0.87 0.84 0.9 0.88 0.81 0.83 0.86 0.85 0.92 0.94 0.89 0.78 0.75 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0.05 0.06 0.11 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Bud 1 0 0 1 1 0 1 2 2 4 4 3 0 0 0 1 0 0 0 0 2 1 4 1
Bud Neck 0 0 0 0 2 0 1 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 4 2 8 7 15 6 16 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 1 15 9 8 16 14 31 8 18 4 9 9 27 43 16 13 13 0 0 1 5 5 5
Endoplasmic Reticulum 1 0 2 0 0 0 0 0 0 0 0 0 2 1 0 1 5 6 0 0 2 0 2 1
Endosome 0 0 2 0 4 1 2 2 0 1 1 0 0 1 5 3 2 2 2 0 5 1 4 5
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 1 1 3 0 0 0 0 0 3 2 5 4
Mitochondria 4 26 3 5 1 46 61 62 128 119 130 199 9 11 9 7 5 11 0 2 5 13 9 22
Nucleus 36 83 373 250 442 416 469 511 281 337 382 439 163 298 283 109 147 162 68 89 360 168 332 242
Nuclear Periphery 0 0 1 2 5 3 7 12 8 9 3 6 2 5 7 4 6 2 0 0 0 0 0 2
Nucleolus 0 1 0 2 1 3 4 12 16 9 15 14 1 2 9 4 0 5 0 0 0 5 12 18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2 1
SpindlePole 1 0 1 1 3 1 3 4 1 3 0 2 1 5 5 0 1 2 0 0 1 0 6 2
Vac/Vac Membrane 0 1 5 1 8 20 11 26 4 12 5 4 3 5 7 4 9 10 1 3 19 12 49 73
Unique Cell Count 40 90 395 264 463 469 517 589 340 405 438 520 182 339 349 132 171 191 74 96 406 217 442 394
Labelled Cell Count 44 112 402 271 476 510 576 670 455 529 550 693 192 356 371 149 189 213 74 96 406 217 442 394


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.3 6.8 6.3 6.9 5.4 5.3 5.7 4.4 4.8 4.6 4.8 6.3 6.3 6.1 6.6 7.7 7.5 7.4 7.5 7.5
Std Deviation (1e-4) 0.9 0.6 1.4 1.4 1.4 1.2 1.1 1.5 1.1 0.9 0.9 1.0 1.2 1.3 1.2 1.8 2.5 2.1 1.8 1.8 1.6
Intensity Change (Log2) – – – -0.11 0.02 -0.32 -0.35 -0.24 -0.61 -0.49 -0.54 -0.5 -0.11 -0.1 -0.15 -0.04 0.18 0.14 0.13 0.14 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.432 5.6727 5.4487 5.1037 4.5635 5.213 4.3275 4.6844 4.1868 4.139 4.0554 4.3486 1.5978 1.8624 1.299 1.157 1.648 1.0611
Actin 0.0323 0 0 0.0263 0 0 0.0194 0 0.0059 0 0.0006 0.0003 0.0196 0 0.0153 0.0699 0.043 0.0001
Bud 0.0006 0 0 0.0002 0 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0005 0 0.0016 0.0002 0.0007 0
Bud Neck 0.0027 0.0002 0.0002 0.0001 0.0002 0.0012 0.0003 0.0001 0.0002 0.0001 0.0005 0.0021 0.0025 0.0001 0.0008 0.0041 0.0015 0.0007
Bud Periphery 0.0014 0 0 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0003 0.0009 0 0.003 0.0002 0.0008 0.0001
Bud Site 0.0017 0.0001 0 0.0005 0 0.0001 0.0003 0.0002 0.0005 0 0.0002 0.0001 0.0037 0 0.0033 0.0019 0.0114 0.0001
Cell Periphery 0.0002 0 0 0 0 0 0.0001 0.0001 0 0 0.0001 0.0001 0.0002 0 0.0003 0.0002 0.0004 0
Cytoplasm 0.0025 0 0.0002 0.0001 0 0.0001 0.0275 0.0001 0.0002 0.0036 0.0003 0.0004 0.0031 0.0001 0.004 0.0008 0.0118 0.0001
Cytoplasmic Foci 0.0036 0 0 0.0042 0 0 0.0197 0 0.002 0 0.0113 0 0.0112 0 0.0096 0.0044 0.0523 0.001
Eisosomes 0.0004 0 0 0.0001 0 0 0.0002 0 0 0 0 0 0.0001 0 0.0001 0.0009 0.0004 0
Endoplasmic Reticulum 0.0015 0 0.0001 0.0001 0 0 0.0143 0 0.0001 0.0001 0.0002 0.0001 0.0014 0 0.0022 0.0003 0.0176 0
Endosome 0.0048 0 0.0001 0.0013 0 0 0.029 0 0.0023 0.0001 0.0108 0.0004 0.0174 0 0.0174 0.0013 0.0707 0.0003
Golgi 0.0022 0 0 0.0021 0 0 0.0057 0 0.0047 0 0.0073 0 0.0037 0 0.0109 0.0036 0.0157 0.0004
Lipid Particles 0.0029 0 0 0.0065 0 0 0.0087 0 0.0008 0 0.0149 0.0001 0.0049 0 0.0072 0.002 0.0699 0.0015
Mitochondria 0.0025 0.0001 0.0003 0.0016 0.0002 0.0001 0.0033 0.0003 0.0081 0.0004 0.0015 0.0027 0.0019 0.0002 0.0064 0.0009 0.0039 0.0014
None 0.0102 0 0.0001 0.0006 0 0 0.019 0 0.0001 0.0141 0.0001 0.0003 0.0028 0 0.0021 0.0003 0.0127 0
Nuclear Periphery 0.0119 0.0017 0.0035 0.001 0.0006 0.0003 0.0647 0.0018 0.0035 0.0105 0.0026 0.0049 0.0068 0.0014 0.0084 0.0213 0.0354 0.0002
Nucleolus 0.0118 0.0336 0.0301 0.0117 0.02 0.043 0.0095 0.031 0.0197 0.014 0.0213 0.041 0.0092 0.0092 0.0077 0.0056 0.0281 0.0248
Nucleus 0.8832 0.9632 0.9641 0.9203 0.9784 0.9543 0.7605 0.9653 0.9399 0.9528 0.9174 0.9424 0.8702 0.9885 0.8896 0.8715 0.5729 0.9602
Peroxisomes 0.0069 0 0 0.0198 0 0 0.0034 0 0.0109 0 0.0082 0.0001 0.0023 0 0.0042 0.0097 0.0083 0.009
Punctate Nuclear 0.0143 0.0008 0.0008 0.0031 0.0002 0.0005 0.0096 0.0002 0.0003 0.0036 0.0009 0.0037 0.0319 0.0001 0.0037 0.0007 0.0378 0.0001
Vacuole 0.0013 0.0002 0.0002 0.0002 0.0001 0.0002 0.0015 0.0004 0.0003 0.0002 0.001 0.0005 0.0049 0.0001 0.0016 0.0001 0.0025 0.0001
Vacuole Periphery 0.0012 0.0001 0.0002 0.0001 0.0001 0 0.003 0.0002 0.0002 0.0002 0.0004 0.0004 0.001 0.0001 0.0008 0.0002 0.0021 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.2731 7.0708 9.6192 6.6324 5.9822 17.1282 8.0429 10.4548 8.8156 6.3779
Translational Efficiency 1.9536 1.8417 1.2097 1.8103 2.5621 0.7213 1.1577 1.3863 1.3851 2.1013

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2285 1228 1822 102 869 2476 1422 1585 3154 3704 3244 1687

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 764.87 813.41 934.87 1230.63 639.26 851.09 898.34 1090.92 730.26 838.60 918.86 1099.37
Standard Deviation 94.84 131.37 160.17 179.76 104.84 137.69 149.64 158.76 112.67 136.78 156.69 163.53
Intensity Change Log 2 — 0.088768 0.289551 0.686111 — 0.412909 0.490859 0.771071 — 0.245412 0.384696 0.725412

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000053 0.000450 0.000490 0.001389 0.000168 0.000103 0.000542 0.000715 0.000085 0.000218 0.000513 0.000756
Bud Neck 0.001885 0.007988 0.012384 0.023877 0.000762 0.007010 0.006905 0.022972 0.001575 0.007334 0.009982 0.023027
Bud Site 0.000316 0.001663 0.001001 0.007363 0.000335 0.001206 0.001209 0.002682 0.000321 0.001358 0.001092 0.002965
Cell Periphery 0.000294 0.000389 0.000101 0.000106 0.000371 0.000276 0.000309 0.000119 0.000315 0.000314 0.000192 0.000119
Cytoplasm 0.005630 0.009129 0.001145 0.000732 0.011766 0.004389 0.009192 0.001719 0.007320 0.005960 0.004672 0.001659
Cytoplasmic Foci 0.001358 0.001225 0.000014 0.000028 0.002657 0.000835 0.001412 0.000655 0.001716 0.000964 0.000626 0.000617
Eisosomes 0.000013 0.000024 0.000025 0.000041 0.000018 0.000019 0.000046 0.000032 0.000015 0.000021 0.000034 0.000033
Endoplasmic Reticulum 0.004221 0.011921 0.004853 0.004749 0.003345 0.006668 0.005243 0.004693 0.003980 0.008409 0.005024 0.004697
Endosome 0.000317 0.001767 0.000159 0.000224 0.000396 0.000837 0.000734 0.000926 0.000339 0.001145 0.000411 0.000884
Golgi 0.000095 0.000672 0.000036 0.000082 0.000293 0.000143 0.000242 0.000717 0.000150 0.000318 0.000126 0.000678
Lipid Particles 0.000479 0.000379 0.000031 0.000104 0.001062 0.000199 0.000644 0.000085 0.000640 0.000259 0.000299 0.000086
Mitochondria 0.000539 0.002262 0.001505 0.006357 0.001756 0.002959 0.001548 0.002635 0.000874 0.002727 0.001523 0.002860
Mitotic Spindle 0.000690 0.001165 0.001138 0.000437 0.000045 0.002291 0.002370 0.004620 0.000512 0.001918 0.001678 0.004367
None 0.003004 0.007993 0.001452 0.001463 0.023850 0.006417 0.025488 0.001970 0.008748 0.006940 0.011988 0.001940
Nuclear Periphery 0.000932 0.001032 0.000868 0.000517 0.000567 0.001723 0.000403 0.001269 0.000832 0.001494 0.000664 0.001224
Nuclear Periphery Foci 0.000172 0.000904 0.000106 0.000038 0.000427 0.000329 0.000304 0.000534 0.000242 0.000520 0.000193 0.000504
Nucleolus 0.003466 0.008057 0.002503 0.015707 0.009734 0.003591 0.006357 0.006626 0.005193 0.005072 0.004192 0.007175
Nucleus 0.975641* 0.939929* 0.970483* 0.932886* 0.941107* 0.957547* 0.934654* 0.942235* 0.966126* 0.951706* 0.954778* 0.941670*
Peroxisomes 0.000203 0.000751 0.000071 0.000463 0.000510 0.000263 0.000669 0.000142 0.000288 0.000425 0.000333 0.000161
Vacuole 0.000615 0.001990 0.001380 0.003191 0.000712 0.002268 0.001453 0.003135 0.000642 0.002176 0.001412 0.003138
Vacuole Periphery 0.000077 0.000312 0.000258 0.000244 0.000118 0.000927 0.000277 0.001520 0.000088 0.000723 0.000266 0.001442

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.66 -11.43 -7.67 -5.68 -5.26 3.32 -7.04 -13.32 -15.68 -2.70 -8.26 -13.57 -17.58 -13.82 -5.06
Bud Neck -5.72 -14.74 -6.84 -5.04 -3.79 -8.10 -12.45 -21.04 -13.42 -14.35 -8.99 -17.98 -21.16 -14.42 -12.45
Bud Site -4.66 -4.50 -1.79 -1.30 -1.48 -3.40 -3.95 -3.77 -2.18 -1.98 -5.36 -5.94 -4.18 -2.29 -2.52
Cell Periphery -0.49 1.50 1.44 1.92 -0.08 0.53 0.40 1.50 4.69 4.95 0.02 1.19 2.00 3.83 3.83
Cytoplasm -2.26 6.73 2.91 3.97 -0.64 3.86 1.19 5.25 3.89 5.29 1.41 2.89 7.01 5.86 4.06
Cytoplasmic Foci 0.17 2.63 2.59 2.76 -2.03 2.16 1.78 2.74 1.05 2.33 1.49 2.64 2.71 1.59 0.63
Eisosomes -4.91 -6.96 -5.82 -4.28 -4.07 -0.14 -5.64 -6.01 -5.53 3.12 -3.28 -8.41 -10.88 -6.72 0.74
Endoplasmic Reticulum -10.94 -2.47 -1.80 7.31 -0.71 -7.21 -3.80 -4.15 4.69 1.66 -11.16 -3.90 -2.44 9.64 1.84
Endosome -2.11 2.16 0.39 2.16 -1.61 -1.82 -1.49 -1.17 0.40 0.15 -2.98 -0.84 -1.58 1.36 -1.14
Golgi -1.21 1.09 -1.48 1.03 -4.46 1.08 0.35 -0.54 -1.09 -0.75 -0.96 0.31 -1.06 -0.52 -1.13
Lipid Particles 0.37 1.80 1.28 2.97 -2.75 1.59 0.72 1.96 1.58 1.75 1.46 1.27 2.46 2.72 1.53
Mitochondria -3.57 -3.50 -1.93 -1.04 -1.35 -0.91 0.11 -0.72 0.43 -1.76 -3.28 -1.93 -3.69 0.00 -2.35
Mitotic Spindle -0.87 -0.95 -1.86 -1.63 -1.53 -3.11 -2.96 -3.68 -1.51 -1.25 -2.33 -2.29 -3.34 -2.02 -2.03
None -3.22 3.50 0.25 2.93 -1.32 5.17 -0.02 6.70 4.52 7.61 1.50 -1.60 6.85 5.85 7.11
Nuclear Periphery -0.73 0.09 0.58 1.07 0.21 -2.69 0.53 -2.17 1.07 -3.42 -2.52 0.76 -1.73 0.79 -2.02
Nuclear Periphery Foci -1.36 0.45 0.84 1.55 0.75 0.68 0.60 0.18 -0.47 -0.40 -1.29 0.31 -0.91 0.56 -1.19
Nucleolus -5.33 0.34 -2.78 -1.85 -2.81 5.61 2.83 1.22 -5.06 -1.72 0.16 1.06 -3.87 -3.97 -4.49
Nucleus 8.34 3.23 4.60 1.52 3.91 -2.91 0.81 0.18 4.49 -0.84 4.88 4.40 7.84 3.62 4.09
Peroxisomes -2.45 1.98 -2.87 0.53 -4.04 1.65 -0.21 2.29 1.48 1.66 -1.06 -0.22 1.72 2.72 1.12
Vacuole -5.06 -2.69 -3.57 -2.25 -2.57 -4.45 -3.15 -3.65 -1.00 -1.91 -6.44 -4.07 -4.26 -1.40 -2.29
Vacuole Periphery -4.98 -2.29 -3.34 1.23 0.36 -2.69 -1.67 -2.30 -0.37 -1.91 -3.16 -2.94 -2.40 -0.76 -1.93
Standard name
Human Ortholog
Description Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ("nineteen complex") and acts after Prp2p to promote the first catalytic reaction of splicing
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Yju2

Yju2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yju2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available