ORF
Human Ortholog
Description Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.97 0.97 0.93 0.91 0.77 0.83 0.76 0.71 0.5 0.59 0.99 1.0 0.99 – 0.97 0.92 0.94 0.9 0.87 0.9 0.86 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0.06 0.07 0.15 0.27 0.4 0.27 0.41 0.48 0.7 0.7 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – 0 1 0 2 3 0 2 1
Bud 0 2 1 0 3 1 0 4 1 2 0 3 1 1 0 – 0 1 1 0 1 0 3 4
Bud Neck 0 0 2 0 0 1 0 1 1 2 0 0 0 0 0 – 1 0 1 1 0 2 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 3 3 0 0 0 1 0 0 2 1 1 1 1 5 – 3 3 0 0 1 0 0 1
Cytoplasm 226 345 168 116 109 107 149 186 130 155 99 105 171 356 269 – 312 195 214 324 155 136 196 162
Endoplasmic Reticulum 3 2 0 1 1 0 2 1 1 1 3 0 1 0 1 – 19 15 0 0 1 0 1 4
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 – 4 9 2 2 0 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 1 2 2 1 0 1 1
Mitochondria 32 15 10 8 17 32 78 60 70 105 138 125 0 2 1 – 2 1 3 13 6 4 4 6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – 0 1 0 0 0 0 1 0
Nuclear Periphery 0 0 0 1 0 0 2 0 1 0 2 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 – 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 1 1 1 0 0 0 0 – 0 2 0 1 0 1 1 1
Vac/Vac Membrane 0 5 0 0 1 2 2 1 1 0 0 1 1 0 1 – 5 6 0 7 1 0 6 8
Unique Cell Count 233 357 174 120 117 117 193 223 171 217 197 179 173 357 273 321 211 229 362 178 151 227 205
Labelled Cell Count 262 373 184 126 131 144 234 255 206 268 244 237 176 360 280 346 236 229 362 178 151 227 205


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.3 3.6 3.6 3.4 3.2 2.6 2.9 2.9 2.5 2.6 2.4 5.2 4.8 4.9 4.8 6.2 6.1 4.1 4.4 5.0
Std Deviation (1e-4) 0.6 0.7 0.8 0.9 1.2 1.5 0.9 0.9 0.9 0.8 0.6 0.4 2.0 1.4 1.6 0.9 1.5 1.5 1.0 1.4 1.1
Intensity Change (Log2) – – – -0.03 -0.09 -0.18 -0.49 -0.3 -0.35 -0.52 -0.48 -0.6 0.52 0.41 0.43 0.39 0.76 0.75 0.16 0.29 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5136 3.0567 2.8677 2.8449 2.6382 2.7501 2.1163 2.5525 2.0139 0.9831 2.2327 2.3411 7.3455 2.3914 2.5496 3.0411 3.1899 2.4865
Actin 0.0289 0.0229 0.0205 0.0798 0.0017 0.0035 0.0343 0.0198 0.0045 0.0006 0.0016 0.0136 0.221 0.0277 0.0248 0.0724 0.0006 0.0184
Bud 0.0001 0.0002 0.0002 0.0004 0.0002 0.0016 0.0008 0.0003 0.0037 0.0002 0.0002 0.0004 0.0034 0.0053 0.0019 0.0005 0.0014 0.0012
Bud Neck 0.0004 0.0003 0.001 0.0002 0.0002 0.0007 0.0042 0.0002 0.0003 0.0002 0.0002 0.0003 0.0009 0.0003 0.0001 0.0001 0.0005 0.0003
Bud Periphery 0.0002 0.0002 0.0003 0.0003 0.0003 0.0027 0.0015 0.0005 0.0099 0.0003 0.0002 0.0005 0.0059 0.0021 0.0037 0.0019 0.0006 0.0035
Bud Site 0.0006 0.0011 0.0005 0.001 0.0002 0.0003 0.0242 0.0099 0.0008 0.0002 0.0004 0.0008 0.0041 0.0203 0.001 0.0004 0.0192 0.0001
Cell Periphery 0.0008 0.0003 0.0005 0.0001 0.0001 0.0002 0.0007 0.0003 0.0002 0.0002 0.0001 0.0001 0.0009 0.0004 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.2524 0.5644 0.5341 0.5168 0.4016 0.55 0.2852 0.4216 0.405 0.5241 0.4538 0.3872 0.009 0.4647 0.3816 0.07 0.2546 0.4053
Cytoplasmic Foci 0.0087 0.0103 0.0044 0.0122 0.0106 0.0099 0.0437 0.0311 0.0085 0.0535 0.006 0.0129 0.0462 0.0169 0.0293 0.0197 0.0697 0.007
Eisosomes 0.0002 0.0006 0.0005 0.0002 0.0001 0.0001 0.001 0.0002 0.0001 0 0.0001 0.0001 0.0029 0.0004 0.0003 0.0009 0 0.0001
Endoplasmic Reticulum 0.0145 0.0052 0.0044 0.0024 0.0016 0.0035 0.0025 0.0018 0.0017 0.0042 0.0017 0.001 0.0318 0.004 0.0013 0.0009 0.01 0.002
Endosome 0.0128 0.0074 0.0038 0.0057 0.028 0.0103 0.0123 0.01 0.0243 0.0239 0.0047 0.0087 0.0648 0.0058 0.004 0.0029 0.1901 0.0007
Golgi 0.0021 0.0129 0.0006 0.0064 0.0127 0.0027 0.0122 0.0028 0.011 0.0014 0.0043 0.0127 0.0225 0.0086 0.0041 0.0085 0.0204 0.0002
Lipid Particles 0.0084 0.0009 0.0008 0.0019 0.0005 0.0016 0.0112 0.003 0.0042 0.0037 0.0015 0.0045 0.024 0.0022 0.0031 0.0146 0.0029 0.0003
Mitochondria 0.0008 0.0025 0.0007 0.0019 0.0252 0.0011 0.0422 0.003 0.0077 0.0005 0.0753 0.0139 0.0271 0.001 0.0244 0.005 0.0068 0.0001
None 0.6542 0.3673 0.4003 0.3609 0.5123 0.4083 0.49 0.4821 0.5121 0.3648 0.4441 0.5207 0.2443 0.4346 0.4883 0.7412 0.2608 0.5594
Nuclear Periphery 0.0037 0.0005 0.0094 0.0005 0.0004 0.0005 0.0005 0.0005 0.0005 0.0031 0.0003 0.0003 0.0601 0.0008 0.0003 0.0003 0.0938 0.0002
Nucleolus 0.0001 0.0001 0.0011 0 0.0001 0 0.0005 0.0001 0.0001 0.0002 0.0001 0.0001 0.0011 0.0001 0.0001 0.0001 0.0007 0
Nucleus 0.0008 0.0006 0.0063 0.0005 0.0006 0.0007 0.0007 0.0005 0.0006 0.0008 0.0006 0.0004 0.0078 0.001 0.0005 0.0001 0.0116 0.0004
Peroxisomes 0.0077 0.001 0.0006 0.0078 0.0003 0.0004 0.0281 0.0095 0.0017 0.0025 0.0036 0.0203 0.1747 0.0015 0.0296 0.0593 0.003 0.0002
Punctate Nuclear 0.0008 0.0002 0.0075 0.0005 0.0009 0.0003 0.0016 0.001 0.0003 0.0129 0.0002 0.0007 0.0026 0.0005 0.0005 0.0007 0.0013 0.0002
Vacuole 0.0014 0.0008 0.0017 0.0006 0.0015 0.0013 0.002 0.0014 0.002 0.0022 0.0009 0.0005 0.0209 0.0015 0.0007 0.0002 0.0288 0.0003
Vacuole Periphery 0.0003 0.0001 0.0006 0.0001 0.0011 0.0003 0.0004 0.0004 0.0007 0.0005 0.0002 0.0002 0.0239 0.0002 0.0001 0.0001 0.023 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.3757 8.3158 10.9173 9.5145 7.886 4.291 7.923 8.9488 10.026 9.663
Translational Efficiency 0.4726 0.733 0.6039 0.6203 0.6573 1.2322 0.9534 0.8546 0.6581 0.6772

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1944 396 814 2587 1903 1538 380 2847 3847 1934 1194 5434

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 678.38 737.05 888.54 917.66 704.15 733.90 990.16 987.83 691.13 734.54 920.88 954.42
Standard Deviation 72.00 77.34 106.27 120.73 75.54 92.38 97.74 121.46 74.89 89.51 113.93 126.08
Intensity Change Log 2 — 0.119669 0.389343 0.435866 — 0.059701 0.491779 0.488380 — 0.089437 0.442424 0.462851

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000055 0.000762 0.000227 0.000913 0.000162 0.000301 0.000285 0.000390 0.000108 0.000396 0.000245 0.000639
Bud Neck 0.016119 0.030893 0.002469 0.010232 0.011499 0.026896 0.001228 0.012289 0.013834 0.027715 0.002074 0.011310
Bud Site 0.004134 0.024793 0.009651 0.043104 0.003310 0.016849 0.004216 0.033322 0.003726 0.018475 0.007921 0.037979
Cell Periphery 0.000186 0.000108 0.000110 0.000125 0.000123 0.000115 0.000126 0.000057 0.000155 0.000114 0.000115 0.000090
Cytoplasm 0.566907* 0.422397* 0.563217* 0.640585* 0.548961* 0.487892* 0.490127* 0.735784* 0.558030* 0.474481* 0.539955* 0.690462*
Cytoplasmic Foci 0.184510* 0.291829* 0.024998 0.009831 0.224674* 0.247508* 0.023168 0.005490 0.204378* 0.256583* 0.024416 0.007557
Eisosomes 0.000195 0.000119 0.000041 0.000018 0.000154 0.000112 0.000060 0.000011 0.000175 0.000113 0.000047 0.000015
Endoplasmic Reticulum 0.001159 0.000828 0.001808 0.000753 0.000637 0.000458 0.003163 0.000196 0.000901 0.000534 0.002239 0.000462
Endosome 0.011496 0.036436 0.004667 0.001891 0.011907 0.031575 0.007194 0.002690 0.011699 0.032570 0.005471 0.002310
Golgi 0.001525 0.005091 0.000090 0.002135 0.002245 0.005593 0.000117 0.001324 0.001881 0.005490 0.000098 0.001710
Lipid Particles 0.008101 0.003876 0.001665 0.000777 0.007148 0.005491 0.003208 0.000209 0.007629 0.005160 0.002156 0.000480
Mitochondria 0.002108 0.005913 0.000254 0.002142 0.003113 0.006952 0.000461 0.005032 0.002605 0.006739 0.000320 0.003656
Mitotic Spindle 0.000542 0.004316 0.001784 0.044251 0.001235 0.007103 0.004384 0.056581 0.000885 0.006532 0.002611 0.050711
None 0.005371 0.000972 0.005673 0.006900 0.002780 0.002192 0.003256 0.006432 0.004089 0.001942 0.004904 0.006655
Nuclear Periphery 0.000449 0.000721 0.001342 0.000878 0.000358 0.000221 0.001457 0.000580 0.000404 0.000324 0.001378 0.000722
Nuclear Periphery Foci 0.000568 0.001444 0.001897 0.000551 0.000276 0.000272 0.004747 0.000158 0.000424 0.000512 0.002804 0.000345
Nucleolus 0.000808 0.000928 0.000488 0.000349 0.000879 0.001060 0.000238 0.000469 0.000843 0.001033 0.000408 0.000412
Nucleus 0.132548* 0.051157 0.274433* 0.167095* 0.121751 0.052800 0.216757* 0.097716 0.127207 0.052464 0.256077* 0.130746*
Peroxisomes 0.001747 0.014666 0.000293 0.001209 0.004139 0.010468 0.000352 0.000649 0.002930 0.011328 0.000312 0.000915
Vacuole 0.060671 0.100790 0.104604 0.065388 0.053830 0.093072 0.235190* 0.039759 0.057287 0.094653 0.146164 0.051960
Vacuole Periphery 0.000801 0.001960 0.000290 0.000874 0.000818 0.003069 0.000268 0.000861 0.000810 0.002842 0.000283 0.000867

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.88 -4.32 -5.25 0.63 -2.54 -1.71 -3.00 -0.76 1.19 2.19 -2.95 -4.32 -4.82 0.39 -1.29
Bud Neck -3.66 11.09 7.15 6.14 -6.17 -7.97 12.77 3.21 9.95 -12.01 -7.89 15.21 7.60 11.98 -10.72
Bud Site -6.52 -1.65 -8.68 1.73 -4.37 -10.47 -1.42 -8.42 1.56 -5.96 -11.89 -2.05 -11.98 1.40 -5.59
Cell Periphery 6.15 6.66 10.42 1.92 2.99 0.67 1.01 11.43 6.45 10.95 3.97 5.47 15.45 5.27 8.54
Cytoplasm 9.94 7.62 7.51 -6.13 -2.59 6.72 10.09 -9.09 -15.01 -15.21 11.31 11.38 -1.80 -12.74 -12.57
Cytoplasmic Foci -9.08 40.10 47.22 25.74 9.15 -3.11 42.53 46.88 44.68 9.23 -8.96 57.01 65.83 51.14 12.67
Eisosomes 5.27 19.73 25.36 7.11 8.39 3.96 11.07 19.37 12.08 14.61 7.35 21.88 31.51 13.43 15.36
Endoplasmic Reticulum 0.78 -5.86 -0.11 -0.94 9.17 1.76 -10.72 3.18 0.53 11.86 2.48 -11.78 1.06 -2.43 15.77
Endosome -6.89 8.65 15.67 10.00 10.06 -10.88 7.46 15.68 18.07 5.89 -13.10 11.83 22.12 20.73 11.27
Golgi -5.10 11.66 3.01 6.06 -1.94 -5.27 9.07 6.84 8.75 -2.54 -7.15 13.47 6.95 9.88 -2.89
Lipid Particles 5.51 9.74 11.60 6.24 5.78 2.01 7.45 13.90 7.10 4.35 3.67 13.01 17.69 7.82 7.81
Mitochondria -1.45 5.75 2.56 1.81 -9.18 -3.60 4.21 1.25 5.23 -5.00 -4.46 6.47 2.14 5.76 -7.64
Mitotic Spindle -1.76 -2.18 -10.84 -6.60 -9.84 -3.88 -1.90 -13.08 -9.09 -6.48 -4.49 -2.57 -17.00 -10.78 -12.84
None 5.77 0.90 -2.76 -21.61* -5.26 1.18 0.83 -14.16 -14.26 -17.01 4.20 0.39 -9.13 -17.57 -10.81
Nuclear Periphery -0.54 -11.95 -14.50 -3.01 -3.36 3.66 -14.89 -22.25 -24.99 -1.60 0.80 -19.02 -23.81 -15.59 -3.31
Nuclear Periphery Foci -1.13 -5.48 -0.22 1.12 8.41 -0.28 -11.25 3.63 3.26 11.53 -0.34 -11.57 0.28 0.74 14.37
Nucleolus -0.70 3.11 3.49 2.44 -0.18 -1.11 7.32 1.84 2.35 -10.15 -1.48 5.82 3.52 3.20 -3.31
Nucleus 16.48 -18.32 -24.21 -35.04 4.69 19.02 -8.83 -17.75 -36.75 1.73 26.89 -20.42 -29.47 -52.80 6.44
Peroxisomes -8.32 10.40 7.79 9.18 -1.10 -6.14 9.09 9.06 10.97 -0.34 -9.99 11.70 11.28 13.57 -0.78
Vacuole -6.73 -12.94 -21.48 -6.94 0.28 -11.64 -18.87 -14.55 -1.93 15.15 -13.03 -21.35 -25.50 -8.46 10.91
Vacuole Periphery -3.38 6.97 3.83 4.65 -0.38 -4.86 7.17 5.58 6.00 -1.29 -5.44 9.50 6.48 6.76 -0.87
ORF
Human Ortholog
Description Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
Localization
Cell Percentages cytoplasm (74%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YIL001W

YIL001W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yil001w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available