Standard name
Human Ortholog
Description Cytoplasmic RNA-binding protein; contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.98 0.99 0.96 0.95 0.91 0.87 0.72 0.79 0.73 0.67 0.99 0.99 0.99 0.97 0.92 0.91 0.79 0.87 0.87 0.91 0.85 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0.07 0.08 0.08 0.11 0.42 0.33 0.4 0.44 0 0 0 0 0 0 0.09 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 2 1 2 1 2 0 0 0 0 0 0 0 2 2 0 0 1
Bud 0 3 0 0 6 3 2 4 2 5 6 6 0 0 0 0 0 0 0 1 0 1 6 2
Bud Neck 0 2 2 1 2 7 8 2 0 1 0 0 0 1 0 0 0 0 0 0 0 1 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 3 1 1 1 5 2 0 6 3 7 3 4 0 5 10 9 0 0 1 0 0 0
Cytoplasm 44 196 222 174 155 327 348 405 192 262 273 238 140 222 178 116 158 120 64 185 206 142 169 145
Endoplasmic Reticulum 0 0 2 0 0 0 0 1 9 1 5 2 0 2 1 5 5 9 0 0 1 0 2 3
Endosome 0 0 0 2 0 0 2 3 0 0 4 2 0 0 1 1 0 1 1 1 1 0 2 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 2 1 0 0 0 0
Mitochondria 3 6 0 3 11 29 32 52 113 109 150 156 1 0 0 2 3 1 7 7 11 4 3 6
Nucleus 0 1 1 1 3 2 4 5 1 1 3 4 1 1 0 0 4 2 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 1 0 5 2 3 3 2 0 0 0 0 0 0 0 3 2 0 3 0
Vac/Vac Membrane 1 0 1 0 0 3 10 19 2 5 7 6 0 0 0 0 4 3 1 2 4 1 3 1
Unique Cell Count 46 203 226 175 161 344 384 463 268 333 376 353 141 225 179 119 171 132 82 213 238 157 200 175
Labelled Cell Count 49 211 232 182 181 373 411 501 322 396 456 425 145 230 180 129 187 146 82 213 238 157 200 175


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 3.8 5.4 4.9 4.3 4.7 4.7 5.1 4.2 4.7 4.6 4.7 5.6 5.7 5.4 6.8 8.3 8.5 5.9 6.5 6.9
Std Deviation (1e-4) 1.2 0.8 1.2 1.2 1.1 1.6 1.3 1.3 1.0 1.1 1.1 1.3 1.6 1.3 1.2 1.5 1.7 1.6 1.8 1.9 2.0
Intensity Change (Log2) – – – -0.15 -0.33 -0.19 -0.19 -0.09 -0.35 -0.2 -0.24 -0.18 0.06 0.07 0.01 0.33 0.62 0.66 0.13 0.27 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4288 -0.066 -0.2631 -0.3592 -0.4564 -0.2352 5.1794 5.0401 5.2941 4.5496 6.2976 5.1772 1.6136 2.093 2.1149 1.8866 1.6965 1.9827
Actin 0.0319 0.0006 0.0008 0.0109 0.0244 0.0018 0.1101 0.0002 0.0217 0.0031 0.0178 0.0074 0.0455 0.001 0.0085 0.0006 0.036 0.0228
Bud 0.0008 0.0005 0.0006 0.0155 0.0003 0.0002 0.0007 0.0011 0.0016 0.0008 0.0049 0.0002 0.0003 0.0005 0.0022 0.0004 0.0002 0.0014
Bud Neck 0.001 0.0002 0.0002 0.0003 0.0009 0.0007 0.0023 0.0004 0.0008 0.0003 0.0017 0.0088 0.0011 0.0006 0.0002 0.0002 0.0003 0.0035
Bud Periphery 0.0011 0.0004 0.0007 0.008 0.0005 0.0003 0.0007 0.0013 0.0027 0.0029 0.0142 0.0002 0.0003 0.0007 0.0044 0.0007 0.0003 0.0041
Bud Site 0.003 0.0025 0.0009 0.0037 0.0003 0.0004 0.0118 0.0219 0.0086 0.0034 0.0043 0.0003 0.001 0.0176 0.0026 0.0004 0.0006 0.0005
Cell Periphery 0.0003 0.0003 0.0002 0.0005 0.0002 0.0001 0.0003 0.0006 0.0005 0.0009 0.0003 0.0002 0.0002 0.0003 0.0003 0.0004 0.0001 0.0001
Cytoplasm 0.4917 0.5961 0.4845 0.5501 0.4587 0.6593 0.3525 0.5418 0.4076 0.6051 0.3303 0.5578 0.3823 0.5374 0.5795 0.5838 0.5446 0.5817
Cytoplasmic Foci 0.0253 0.0172 0.0218 0.0388 0.0221 0.0207 0.0615 0.0081 0.0359 0.0152 0.0703 0.0143 0.0293 0.0093 0.0164 0.0084 0.0104 0.0093
Eisosomes 0.0004 0.0001 0.0001 0.0003 0.0003 0.0001 0.0003 0.0001 0.0002 0.0006 0.0002 0.0002 0.0002 0.0001 0.0002 0 0.0003 0.0001
Endoplasmic Reticulum 0.0081 0.0044 0.0032 0.0041 0.0069 0.0042 0.014 0.002 0.0053 0.005 0.0102 0.0021 0.006 0.0046 0.0036 0.004 0.003 0.0043
Endosome 0.0272 0.0148 0.0145 0.0224 0.0375 0.015 0.0215 0.0024 0.0243 0.0173 0.044 0.0031 0.0256 0.0029 0.0061 0.0045 0.0155 0.0041
Golgi 0.0038 0.0018 0.0009 0.0027 0.0083 0.0008 0.0151 0.0003 0.0134 0.0017 0.0109 0.0007 0.0127 0.0007 0.0007 0.0004 0.027 0.0029
Lipid Particles 0.0021 0.0003 0.0011 0.0007 0.0035 0.0017 0.0065 0.0002 0.0105 0.0056 0.0069 0.0036 0.0085 0.0005 0.0009 0.0009 0.0157 0.0043
Mitochondria 0.003 0.0006 0.0018 0.0046 0.0026 0.0009 0.0027 0.0003 0.0042 0.0289 0.0092 0.0003 0.0019 0.0005 0.0004 0.0003 0.0101 0.0011
None 0.391 0.354 0.4607 0.3247 0.4099 0.2891 0.3469 0.4155 0.4407 0.2991 0.3707 0.3921 0.4618 0.4204 0.3561 0.3893 0.3153 0.3436
Nuclear Periphery 0.0016 0.0009 0.0012 0.0012 0.0114 0.0006 0.0065 0.0003 0.0019 0.001 0.0372 0.0005 0.0016 0.0002 0.0005 0.0011 0.0008 0.0004
Nucleolus 0.0002 0.0001 0.0002 0.0008 0.0003 0.0001 0.0005 0.0002 0.0005 0.0005 0.003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0
Nucleus 0.0009 0.0012 0.0013 0.0022 0.002 0.0008 0.0141 0.0007 0.0009 0.001 0.013 0.0003 0.0005 0.0003 0.0006 0.0008 0.0005 0.0002
Peroxisomes 0.001 0.0002 0.0006 0.0004 0.0005 0.0004 0.0228 0.0001 0.0116 0.0019 0.0179 0.0024 0.0166 0.001 0.0128 0.0001 0.0159 0.0141
Punctate Nuclear 0.0009 0.0005 0.001 0.0037 0.0014 0.0009 0.0058 0.0005 0.0038 0.0004 0.0241 0.0048 0.002 0.0002 0.0015 0.0008 0.0007 0.0007
Vacuole 0.0044 0.003 0.003 0.0035 0.0057 0.0016 0.0026 0.0018 0.0028 0.0041 0.0048 0.0006 0.0022 0.0009 0.0021 0.0024 0.002 0.0005
Vacuole Periphery 0.0006 0.0004 0.0006 0.0008 0.0025 0.0004 0.0007 0.0002 0.0005 0.0011 0.004 0.0001 0.0004 0.0001 0.0002 0.0003 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.5148 9.1695 14.5804 15.9953 11.3993 8.7872 14.5691 10.7271 15.8652 15.4252
Translational Efficiency 1.7118 3.1573 1.672 1.6206 3.1947 5.2556 1.8975 2.5878 1.9475 2.5276

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1203 371 2798 1495 2074 1864 172 1361 3277 2235 2970 2856

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 658.64 815.94 880.99 906.67 729.70 835.28 1060.00 892.60 703.61 832.07 891.36 899.97
Standard Deviation 78.21 102.84 111.74 133.73 102.79 101.23 134.12 172.56 100.53 101.76 120.64 153.63
Intensity Change Log 2 — 0.308973 0.419636 0.461087 — 0.194956 0.538689 0.290710 — 0.250171 0.483432 0.374051

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000247 0.000758 0.000127 0.000988 0.000077 0.000430 0.000504 0.000328 0.000140 0.000484 0.000148 0.000674
Bud Neck 0.030984 0.036664 0.004483 0.004335 0.006905 0.040375 0.002255 0.004454 0.015744 0.039759 0.004354 0.004392
Bud Site 0.013070 0.049062 0.006235 0.031038 0.006408 0.025912 0.016547 0.028974 0.008854 0.029755 0.006833 0.030054
Cell Periphery 0.000314 0.000178 0.000042 0.000096 0.000119 0.000170 0.000255 0.000557 0.000191 0.000172 0.000054 0.000316
Cytoplasm 0.411704* 0.361293* 0.715715* 0.486961* 0.625640* 0.459722* 0.433247* 0.616819* 0.547103* 0.443383* 0.699356* 0.548843*
Cytoplasmic Foci 0.360948* 0.328682* 0.027147 0.042580 0.180290* 0.263797* 0.095826 0.065702 0.246610* 0.274568* 0.031124 0.053598
Eisosomes 0.000537 0.000157 0.000015 0.000032 0.000063 0.000262 0.000080 0.000068 0.000237 0.000244 0.000019 0.000049
Endoplasmic Reticulum 0.001950 0.000752 0.000633 0.001168 0.001480 0.000672 0.002716 0.000753 0.001652 0.000685 0.000753 0.000970
Endosome 0.018784 0.037069 0.002719 0.020806 0.010627 0.028098 0.020965 0.008319 0.013621 0.029587 0.003775 0.014856
Golgi 0.011650 0.011538 0.000182 0.008465 0.002614 0.009569 0.000367 0.001629 0.005931 0.009896 0.000193 0.005207
Lipid Particles 0.015358 0.004032 0.000611 0.001033 0.004386 0.004811 0.003344 0.001588 0.008414 0.004682 0.000769 0.001298
Mitochondria 0.016174 0.010357 0.000478 0.004508 0.003052 0.010819 0.000074 0.000428 0.007869 0.010742 0.000455 0.002564
Mitotic Spindle 0.000808 0.005324 0.006022 0.065516 0.000883 0.004574 0.000769 0.016594 0.000855 0.004698 0.005718 0.042203
None 0.002975 0.001069 0.001987 0.002959 0.006131 0.001578 0.000764 0.008394 0.004972 0.001493 0.001916 0.005549
Nuclear Periphery 0.000294 0.000139 0.000839 0.000676 0.000242 0.000114 0.000678 0.000340 0.000261 0.000118 0.000830 0.000516
Nuclear Periphery Foci 0.000208 0.000447 0.000595 0.001338 0.000333 0.000150 0.009252 0.001276 0.000287 0.000199 0.001096 0.001309
Nucleolus 0.000773 0.000634 0.000264 0.001187 0.000826 0.000906 0.000214 0.000772 0.000807 0.000861 0.000262 0.000989
Nucleus 0.062123 0.043900 0.116363 0.048923 0.085069 0.047561 0.067625 0.066953 0.076646 0.046953 0.113541 0.057515
Peroxisomes 0.010798 0.023514 0.000233 0.002901 0.001140 0.011200 0.000810 0.001102 0.004685 0.013244 0.000266 0.002044
Vacuole 0.037708 0.080282 0.115048 0.267243* 0.060561 0.086705 0.343159* 0.172464 0.052172 0.085639 0.128258 0.222077*
Vacuole Periphery 0.002593 0.004150 0.000262 0.007246 0.003155 0.002574 0.000548 0.002486 0.002948 0.002836 0.000279 0.004978

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.02 2.31 -1.50 1.45 -2.11 -7.26 -5.91 -4.43 1.88 2.93 -7.57 -0.84 -3.04 0.97 -2.44
Bud Neck -1.30 12.67 13.31 8.47 1.18 -16.51 10.46 5.22 17.87 -2.76 -12.24 12.37 14.21 20.07 0.75
Bud Site -6.43 4.99 -1.65 5.57 -5.86 -11.66 -3.96 -7.62 0.78 -2.62 -12.12 3.40 -7.19 4.37 -9.12
Cell Periphery 2.88 6.50 5.83 5.14 -2.82 -5.08 -4.97 -1.38 -1.12 -0.67 1.08 8.61 -0.32 -0.52 -1.76
Cytoplasm 3.33 -27.80 -3.39 -5.57 22.53 20.07 11.17 3.01 -13.74 -9.47 13.97 -13.97 3.77 -9.37 16.30
Cytoplasmic Foci 2.50 50.24 49.33 25.07 -1.81 -13.43 12.51 26.51 37.77 2.41 -4.71 55.46 50.58 48.79 -7.86
Eisosomes 9.18 13.32 13.01 9.70 -8.18 -10.60 -3.29 1.27 10.63 3.60 -0.34 14.40 12.87 12.74 -7.24
Endoplasmic Reticulum 2.74 2.67 2.51 -0.76 -0.47 3.69 -3.72 4.01 -0.11 5.76 4.50 3.29 4.29 -0.99 2.54
Endosome -4.45 15.78 5.86 6.64 -6.02 -12.04 -1.98 5.15 14.80 3.82 -11.41 18.71 6.46 14.69 -6.82
Golgi 0.18 12.08 6.90 4.38 -4.46 -10.37 15.39 3.01 10.48 -1.79 -5.47 15.48 6.28 9.98 -4.88
Lipid Particles 8.94 14.34 14.11 4.16 -3.42 -1.07 1.92 9.04 10.26 4.75 7.16 17.41 16.65 12.13 -2.68
Mitochondria 1.87 8.66 7.56 3.03 -3.33 -5.79 6.97 6.32 8.24 -3.11 -2.07 10.20 8.67 8.09 -3.07
Mitotic Spindle -1.92 -4.85 -10.02 -7.67 -8.54 -3.36 -0.91 -5.29 -3.47 -5.01 -3.94 -5.12 -11.26 -9.04 -8.71
None 2.72 1.79 1.00 -2.13 -0.84 4.86 6.20 0.01 -3.47 -4.24 5.23 5.25 1.01 -3.67 -3.48
Nuclear Periphery 1.75 -9.44 -8.06 -14.98 1.54 6.65 -7.21 -3.46 -6.33 5.04 4.34 -15.16 -10.24 -16.56 6.56
Nuclear Periphery Foci -0.06 -0.96 -2.05 -3.51 -2.12 3.50 -11.01 -6.57 -8.48 9.80 1.83 -6.10 -5.61 -9.66 0.46
Nucleolus 0.64 3.14 0.23 -0.12 -1.30 -0.37 4.87 1.32 1.35 -2.89 -0.34 5.34 0.84 0.95 -2.08
Nucleus 3.29 -19.11 -1.47 -4.27 17.27 12.27 0.76 4.48 -5.00 1.03 11.73 -16.77 3.43 -7.21 18.49
Peroxisomes -3.38 12.08 10.18 6.13 -3.69 -14.14 2.70 0.98 13.81 -0.67 -9.41 13.26 9.17 14.00 -4.53
Vacuole -7.25 -28.27 -41.13 -28.45 -24.87 -7.62 -15.99 -22.83 -18.34 8.30 -11.76 -28.10 -44.02 -35.52 -19.61
Vacuole Periphery -1.00 9.92 -0.57 0.66 -3.98 1.42 8.45 2.28 1.36 -2.60 0.33 12.32 1.07 0.72 -4.93
Standard name
Human Ortholog
Description Cytoplasmic RNA-binding protein; contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation
Localization
Cell Percentages cytoplasm (71%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sgn1

Sgn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sgn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available